SANTA CRUZ BIOTECHNOLOGY, INC. TMEM53 shRNA Plasmid (m): sc-154478-SH

BACKGROUND CHROMOSOMAL LOCATION TMEM53 (transmembrane 53) is a 247 amino acid protein encoded by Genetic locus: Tmem53 (mouse) mapping to 4 D1. a gene mapping to 1. is the largest human chromosome spanning about 260 million base pairs and making up 8% of the PRODUCT . There are about 3,000 genes on chromosome 1, and consider- TMEM53 shRNA Plasmid (m) is a pool of 3 target-specific lentiviral vector ing the great number of genes there are also a large number of diseases asso- plasmids each encoding 19-25 nt (plus hairpin) shRNAs designed to knock ciated with chromosome 1. Notably, the rare aging disease Hutchinson-Gilford down gene expression. Each plasmid contains a puromycin resistance gene progeria is associated with the LMNA gene which encodes Lamin A. When for the selection of cells stably expressing shRNA. Each vial contains 20 µg defective, the LMNA gene product can build up in the nucleus and cause char- of lyophilized shRNA plasmid DNA. Suitable for up to 20 transfections. Also acteristic nuclear blebs. The mechanism of rapidly enhanced aging is unclear see TMEM53 siRNA (m): sc-154478 and TMEM53 shRNA (m) Lentiviral and is a topic of continuing exploration. The MUTYH gene is located on chro- Particles: sc-154478-V as alternate gene silencing products. mosome 1 and is partially responsible for familial adenomatous polyposis. Stickler syndrome, Parkinson’s, Gaucher disease and Usher syndrome are also STORAGE AND RESUSPENSION associated with chromosome 1. A breakpoint has been identified in 1q which disrupts the DISC1 gene and is linked to schizophrenia. Aberrations in chromo- Store lyophilized shRNA plasmid DNA at 4° C with desiccant. Stable for some 1 are found in a variety of cancers including head and neck cancer, at least one year from the date of shipment. Once resuspended, store at malignant melanoma and multiple myeloma. 4° C for short term storage or -80° C for long term storage. Avoid repeated freeze thaw cycles. REFERENCES Resuspend lyophilized shRNA plasmid DNA in 200 µl of the deionized water 1. Watson, M.L., Kingsmore, S.F., Johnston, G.I., Siegelman, M.H., Le Beau, provided. Resuspension of the shRNA plasmid DNA in 200 µl of deionized M.M., Lemons, R.S., Bora, N.S., Howard, T.A., Weissman, I.L., McEver, R.P. water makes a 0.1 µg/µl solution in a 10 mM Tris, 1 mM EDTA buffered and Seldin, M.F. 1990. Genomic organization of the selectin family of solution. leukocyte adhesion molecules on human and mouse chromosome 1. J. Exp. Med. 172: 263-272. APPLICATIONS 2. Blackwood, D.H., Fordyce, A., Walker, M.T., St Clair, D.M., Porteous, D.J. TMEM53 shRNA Plasmid (m) is recommended for the inhibition of TMEM53 and Muir, W.J. 2001. Schizophrenia and affective disorders—cosegrega- expression in mouse cells. tion with a translocation at chromosome 1q42 that directly disrupts brain- expressed genes: clinical and P300 findings in a family. Am. J. Hum. Genet. SUPPORT REAGENTS 69: 428-433. For optimal shRNA Plasmid transfection efficiency, Santa Cruz Biotechnology’s shRNA Plasmid Transfection Reagent: sc-108061 (0.2 ml) and shRNA Plasmid 3. Weise, A., Starke, H., Mrasek, K., Claussen, U. and Liehr, T. 2005. New Transfection Medium: sc-108062 (20 ml) are recommended. Control shRNAs insights into the evolution of chromosome 1. Cytogenet. Genome Res. are available as 20 µg lyophilized plasmid DNA. Each encodes a scrambled 108: 217-222. shRNA sequence that will not lead to the specific degradation of any known 4. Lans, H. and Hoeijmakers, J.H. 2006. Cell biology: aging nucleus gets out cellular mRNA. Control shRNA Plasmids include: sc-108060, sc-108065 and of shape. Nature 440: 32-34. sc-108066. 5. Gregory, S.G., Barlow, K.F., McLay, K.E., Kaul, R., Swarbreck, D., Dunham, A., Scott, C.E., Howe, K.L., Woodfine, K.C., Spencer, C.A., Jones, M.C., RT-PCR REAGENTS Gillson, C., Searle, S., Zhou, Y., Kokocinski, F., McDonald, L., et al. 2006. Semi-quantitative RT-PCR may be performed to monitor TMEM53 gene The DNA sequence and biological annotation of human chromosome 1. expression knockdown using RT-PCR Primer: TMEM53 (m)-PR: sc-154478-PR Nature 441: 315-321. (20 µl). Annealing temperature for the primers should be 55-60° C and the extension temperature should be 68-72° C. 6. Hennah, W., Thomson, P., Peltonen, L. and Porteous, D. 2006. Genes and schizophrenia: beyond schizophrenia: the role of DISC-1 in major mental RESEARCH USE illness. Schizophr. Bull. 32: 409-416. The purchase of this product conveys to the buyer the nontransferable right 7. Marzin, Y., Jamet, D., Douet-Guilbert, N., Morel, F., Le Bris, M.J., Morice, to use the purchased amount of the product and all replicates and derivatives P., Abgrall, J.F., Berthou, C. and De Braekeleer, M. 2006. Chromosome 1 for research purposes conducted by the buyer in his laboratory only (whether abnormalities in multiple myeloma. Anticancer Res. 26: 953-959. the buyer is an academic or for-profit entity). The buyer cannot sell or other- 8. McClintock, D., Gordon, L.B. and Djabali, K. 2006. Hutchinson-Gilford proge- wise transfer (a) this product (b) its components or (c) materials made using ria mutant Lamin A primarily targets human vascular cells as detected by an this product or its components to a third party, or otherwise use this product anti-Lamin A G608G antibody. Proc. Natl. Acad. Sci. USA 103: 2154-2159. or its components or materials made using this product or its components for Commercial Purposes.

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