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Protein Catalogue SinoBiological Inc. Established in 2007, Sino Biological is a global biotechnology company manufacturing high quality research reagents for basic, translational, and clinical research. Sino Biological laboratories consist of over 80,000 square meters, producing cDNAs, recombinant proteins, antibodies and antibody-based kits that are used to study cellular function and human diseases. Sino Biological scientists are experienced in recombinant protein expression and purification from bacterial, yeast, insect and mammalian system. The scientists have also developed technologies to produce and purify a comprehensive collec- tion of mouse monoclonal antibodies, rabbit polyclonal antibodies, and rabbit monoclonal antibodies. Sino Biological offers integrated service from gene cloning to protein expression, protein purification, and immunization for polyclonal or mono- clonal antibody generation. We have provided CRO services to produce targets proteins, antibodies, and optimized ELISA tools to meet the needs of the biopharmaceutical industry, including the top 10 pharmaceutical companies. Sino Biological product sales are supported through a network of distributors in North American, European and Asian markets. We are proud that our products are helping scientists to solve significant problems in life science research. Every product has to pass rigorous QC test standards to ensure consistent high quality. We validate our product application and guarantee its performance as specified. We are developing tools and technologies to provide the global scientific commu- nity with the best-in-class product to accelerate scientific discovery and innovation. Advantages Best Monoclonal Antibody Tools Worldwide Maximum 200-Fold Enhanced ELISA sensitivity Leading 2nd Generation Rabbit mAb Technology No Products From Origin Entrusted Manufacturer Centralized Protein & Antibody Expression Lab Worldwide 60+ Highly Centralized Cell Culture Bioreactors 6000+ Protein Bulks in Stock 2000+ New Products Released Per Year 30-100 Grams Custom-Tailored mAb Production in Weeks Leading Pre-Clinical CRO for Biologics Worldwide One-stop Service from Discovery to Production Record Speed from DNA to Purified mAbs Serving Top-10 global Pharmaceutical/Biotech Companies Sino Biological Inc. Recombinant Protein 01 Production Service Other CRO Services A Global Leader in Recombinant Protein R&D CONTENTS 04 GMP-level Proteins 05 Biotinylated proteins 09 Viral Proteins 21 Other Proteins Sino Biological PAGE / 01 Sino Biological Inc. Protein All produced in house 1000 protein production per year 85 expressed with mammalian cell Purity: Majority > 95 purity, some > 99 % % % over 6,000 proteins, all in BULK quantities Big discount for bulk order Antibody over 13,000 rabbit/mouse mAbs & rabbit Pabs Most generated against Full-Length proteins 2nd generation of rabbit mAbs 8-156 fold improvement in ELISA detection limit Gene/cDNA clone 400,000+ cDNA clones, sequence confirmed Multiple tags for choice: His, Myc, Flag, HA, GFP and OFP Optimized for protein expression Human, mouse, rat, monkey, dog, virus cDNA available ELISA Kit All Produced in House The detection limit is as low as 3.1 pg/ml Over 500 antibody pairs available Hot Research Tools Transfection Reagent Secondary Antibody Antibody Purification Control Antibody Protein A/G/L Resins Loading Control Protein A/G/L Magnetic Beads Immunoprecipitation (IP) Kit Anti-DYKDDDDK Affinity Resin Protein A/G/L IP Kit Neuraminidase(NA) Tag Antibody IP Kit Protease Primary Antibody IP Kit Nuclease Magnetic Separator Tag Antibody Synthetic Peptide for Elution Cell Lysate Sino Biological 02 / PAGE Recombinant Protein Production Service HEK293 Cell CHO Cell Insect Cell Expression Systems 5Transient/Stable expression Yeast E. coli Technical Strength Extensive Experience with Proteins that are: Optimized expression vectors Secretory High-density cell culture Insoluble Proprietary medium Intracellular Experience of >6000 proteins Enzymes Protein refolding expertise Trans-Membrane Expertise in drug R&D Heterogeneous polymers Other CRO Services Custom Molecular Biology Service Custom Antibody Service Custom Bioanalytical Service Custom Protein Array Service Custom Pharmaceutical Drug Development Service Sino Biological PAGE / 03 A Global Leader in Recombinant Protein R&D Capacity & Capability Milligram level protein production 2L—2000L bioreactor to ensure high throughput manufacture 1000 new proteins made in house per year Only 4 weeks from gene sequence to protein production World Largest Recombinant Protein Bank 6000+ proteins in stock Multiple species & various tags Protein Advantages >85 expressed by eukaryotic cells High purity & low endotoxin % Bioactivity validated Animal Component Free & carrier free HPLC Purity SDS-PAGE Purity VEGF165 Bioactivity PD1 Bioactivity Reduced non-reduced 100 SINO mAu ED =0.94μg/mL 90 OTHER BRAND 4 50 300 80 ) 70 3 250 % 60 200 50 2 150 40 30 100 1 20 Proliferation( 50 10 bs.(OD450mm-Bank) A 0 0 0 5 10 15 20 0.01 0.11 1 10 100 1E-4 1E-3 0.01 0.1 1 10 >97% >96% VEGF165 Conc. (ng/ml) PD1 (μg/mL) TNF-alpha (Cat: GMP-10602-HNAE) Cell (HUVEC) proliferation PD-1 (10377-H03H) bind to PD-L2 Custom Protein Production Services One-stop: gene cloning ->cell culture -> protein purification -> protein characterization High quality: Proprietary technology and optimized process Fast turnaround: From gene sequence to purified protein within 4 weeks in HEK293 cells Low risk: Milestone payments Rich experience: Over 6000 recombinant proteins, from milligram to gram, have been successfully produced. Bacterial Baculovirus-insect cell HEK293/CHO CHO stable cell line Yeast expression transient expression development expression expression Bulk Order, Savings More! In stock & ready to ship Bulk order with amazing discount Sino Biological 04 / PAGE GMP-level Proteins Official Name Alias Species Expression Host Tag Catalog No. CSF2 GM-CSF Human HEK293 Cells Native GMP-10015-HNAH EGF Epidermal Growth Factor Human E. coli Native GMP-10605-HNAE IFNG IFN-gamma Human CHO Stable Cells Native GMP-11725-HNAS IL15 IL-15 Human E. coli Native GMP-10360-HNCE IL1B IL-1 beta Human E. coli Native GMP-10139-HNAE IL2 IL-2 Human E. coli Native GMP-11848-HNAE-B IL4 IL-4 Human E. coli Native GMP-11846-HNAE IL4 IL-4 Human E. coli Native GMP-11846-HNAE-B IL6 IL-6 Human E. coli Native GMP-10395-HNAE IL7 IL-7 Human E. coli Native GMP-11821-HNAE IL12A & IL12B IL-12 (IL12A & IL12B Heterodimer) Human HEK293 Cells C-His GMP-CT011-H08H IL21 IL-21 Human E. coli Native GMP-10584-HNAE TNF TNFA Human E. coli Native GMP-10602-HNAE A-E Sino Biological PAGE / 05 Biotinylated proteins Official Name Alias Species Expression Host Tag Catalog No. ALB Serum Albumin Human Yeast Native 10968-HNAY-B AMIGO2 Human HEK293 Cells C-His 13735-H08H-B ANGPT2 Angiopoietin-2 Human CHO Stable Cells C-His 10691-H08S-B ASGR1 ASGPR1 Mouse HEK293 Cells N-His 50083-M07H-B BTLA Human HEK293 Cells C-human IgG1-Fc & C-AVI 11895-H41H-B BTN3A1 CD277 Human HEK293 Cells C-His 15973-H08H-B C5 C5a Mouse E. coli Native 51136-MNAE-B CD19 Leu-12 Human HEK293 Cells C-His 11880-H08H-B CD22 Siglec-2 Human HEK293 Cells C-His 11958-H08H-B CD22 Siglec-2 Human Baculovirus-Insect Cells C-His 11958-H08H1-B CD226 DNAM-1 Human HEK293 Cells C-human IgG1-Fc 10565-H03H-B CD226 DNAM-1 Human HEK293 Cells C-His 10565-H08H-B CD27 TNFRSF7 Human Baculovirus-Insect Cells C-His 10039-H08B1-B CD27 TNFRSF7 Human HEK293 Cells C-human IgG1-Fc & C-AVI 10039-H41H-B CD274 PD-L1 Human HEK293 Cells C-human IgG1-Fc 10084-H02H-B CD274 PD-L1 Human HEK293 Cells C-His 10084-H08H-B CD274 PD-L1 Mouse HEK293 Cells C-His 50010-M08H-B CD274 PD-L1 Cynomolgus HEK293 Cells C-His 90251-C08H-B CD276 B7-H3 Human HEK293 Cells C-human IgG1-Fc 11188-H02H-B CD276 B7-H3 Human HEK293 Cells C-His 11188-H08H-B CD276 B7-H3 Mouse HEK293 Cells C-His 50973-M08H-B CD28 TP44 Human HEK293 Cells C-human IgG1-Fc & C-AVI 11524-H41H-B CD33 Siglec-3 Human HEK293 Cells C-His 12238-H08H-B CD33 Siglec-3 Cynomolgus HEK293 Cells C-His 90303-C08H-B CD38 Human HEK293 Cells C-His 10818-H08H-B CD3D CD3 delta Human HEK293 Cells C-flag 10981-H23H-B CD3D & CD3E CD3D & CD3E Heterodimer Human HEK293 Cells C-flag & C-His CT038-H2508H-B CD3E CD3 epsilon / CD3e Human CHO Stable Cells C-His 10977-H08S-B CD4 Mouse HEK293 Cells C-His 50134-M08H-B CD40 TNFRSF5 Human HEK293 Cells C-His 10774-H08H-B CD40LG CD40 Ligand Human E. coli C-His 10239-H08E-B CD47 Human HEK293 Cells C-AVI 12283-H27H-B CD47 Human HEK293 Cells C-cleavage 12283-HCCH-B CD80 B7-1 Human HEK293 Cells C-His 10698-H08H-B CD86 B7-2 Human HEK293 Cells C-His & C-human IgG1-Fc 10699-H03H-B CD86 B7-2 Human HEK293 Cells C-His 10699-H08H-B CD86 B7-2 Mouse HEK293 Cells C-His 50068-M08H-B CD8A CD8 alpha Human HEK293 Cells C-His 10980-H08H-B CD8A CD8 alpha Mouse HEK293 Cells C-His 50389-M08H-B CD8B CD8 beta Human HEK293 Cells C-cleavage 11031-HCCH-B CD96 Human HEK293 Cells C-His 11202-H08H-B CEACAM5 CD66e Human HEK293 Cells C-His 11077-H08H-B CEACAM6 CD66c Human HEK293 Cells C-His 10823-H08H-B CRP C-Reactive Protein Human HEK293 Cells Native 11250-HNAH-B CSF1 M-CSF Human HEK293 Cells C-His 11792-H08H-B CSF1 M-CSF Human HEK293 Cells Native 11792-HNAH-B CSF1R CD115 Mouse HEK293 Cells C-His 50059-M08H-B CSF2 GM-CSF Human HEK293 Cells Native 10015-HNAH-B CTLA4 CTLA-4 Human HEK293 Cells C-human IgG1-Fc 11159-H02H6-B CTLA4 CTLA-4 Human HEK293 Cells C-His 11159-H08H-B CTLA4 CTLA-4 Mouse HEK293 Cells C-His 50503-M08H-B EGFR ErbB1 Human HEK293 Cells C-His 10001-H08H-B Sino Biological E-K 06 / PAGE Official Name Alias Species Expression Host Tag Catalog No.
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  • Supplementary Table 1

    Supplementary Table 1

    Supplementary Table 1. 492 genes are unique to 0 h post-heat timepoint. The name, p-value, fold change, location and family of each gene are indicated. Genes were filtered for an absolute value log2 ration 1.5 and a significance value of p ≤ 0.05. Symbol p-value Log Gene Name Location Family Ratio ABCA13 1.87E-02 3.292 ATP-binding cassette, sub-family unknown transporter A (ABC1), member 13 ABCB1 1.93E-02 −1.819 ATP-binding cassette, sub-family Plasma transporter B (MDR/TAP), member 1 Membrane ABCC3 2.83E-02 2.016 ATP-binding cassette, sub-family Plasma transporter C (CFTR/MRP), member 3 Membrane ABHD6 7.79E-03 −2.717 abhydrolase domain containing 6 Cytoplasm enzyme ACAT1 4.10E-02 3.009 acetyl-CoA acetyltransferase 1 Cytoplasm enzyme ACBD4 2.66E-03 1.722 acyl-CoA binding domain unknown other containing 4 ACSL5 1.86E-02 −2.876 acyl-CoA synthetase long-chain Cytoplasm enzyme family member 5 ADAM23 3.33E-02 −3.008 ADAM metallopeptidase domain Plasma peptidase 23 Membrane ADAM29 5.58E-03 3.463 ADAM metallopeptidase domain Plasma peptidase 29 Membrane ADAMTS17 2.67E-04 3.051 ADAM metallopeptidase with Extracellular other thrombospondin type 1 motif, 17 Space ADCYAP1R1 1.20E-02 1.848 adenylate cyclase activating Plasma G-protein polypeptide 1 (pituitary) receptor Membrane coupled type I receptor ADH6 (includes 4.02E-02 −1.845 alcohol dehydrogenase 6 (class Cytoplasm enzyme EG:130) V) AHSA2 1.54E-04 −1.6 AHA1, activator of heat shock unknown other 90kDa protein ATPase homolog 2 (yeast) AK5 3.32E-02 1.658 adenylate kinase 5 Cytoplasm kinase AK7
  • Whole Exome Sequencing to Investigate Genetic Variants of Non-Syndromic Hearing Impairment in a Population of African Ancestry

    Whole Exome Sequencing to Investigate Genetic Variants of Non-Syndromic Hearing Impairment in a Population of African Ancestry

    Whole exome sequencing to investigate genetic variants of non-syndromic hearing impairment in a population of African Ancestry By Noluthando Manyisa MNYNOL006 Submitted to the University of Cape Town in fulfilment of requirements of the degree MSc Med in Human UniversityGenetics of Cape Town University of Cape Town, Faculty of Health Sciences, Department of Pathology, Faculty of Human Genetics Supervisor: Professor Ambroise Wonkam Co-Supervisors: Professor Collet Dandara and Dr Emile Chimusa Word Count: 18 772 Date of Submission: February 2018 1 The copyright of this thesis vests in the author. No quotation from it or information derived from it is to be published without full acknowledgement of the source. The thesis is to be used for private study or non- commercial research purposes only. Published by the University of Cape Town (UCT) in terms of the non-exclusive license granted to UCT by the author. University of Cape Town Table of Contents Plagiarism declaration ................................................................................................................ I Acknowledgments..................................................................................................................... II List of Figures and Tables........................................................................................................ III Figures.................................................................................................................................. III Tables ..................................................................................................................................