Applied Biosystems Food Pathogen Detection System
TaqMan ® Cronobacter sakazakii Detection Kit
Simple, reliable, and rapid detection
TaqMan® Pathogen Detection Kits
• Cronobacter sakazakii
• Salmonella enterica
• E. coli O157:H7
• Listeria monocytogenes
• Pseudomonas aeruginosa
• Staphylococcus aureus
An Infant Formula Contaminant Real-Time PCR Delivers Detect E. sakazakii Faster Cronobacter sakazakii is a pathogen The TaqMan Cronobacter sakazakii A number of tests are currently mostly associated with powdered Detection Kit uses proven real-time available that identify Cronobacter infant formula, causing seizures, brain PCR technology designed to provide: sakazakii based on phenotypic and abscesses, developmental delay, and biochemical methods. However, • Highly selective identification of even death in infants with premature because of isolates that may escape Cronobacter sakazakii in a wide variety infants and newborns at greatest risk. identification with these methods, faster, of food and finished product samples It is therefore extremely important more reliable methods are needed. that infant formula be tested prior to • Verified performance The TaqMan® Cronobacter sakazakii release to the marketplace. It is currently Detection Kit offers such an alternative. • Ready-to-use convenience unknown how many organisms will cause infection, thus a highly specific • Reduced risk of contamination product testing method is necessary to determine the presence of Cronobacter sakazakii. Rely on Applied Biosystems Use a Complete Solution with Results in three easy steps The TaqMan Cronobacter sakazakii an Expanding Menu Detection Kit has been designed using The TaqMan Cronobacter sakazakii 1. Enrich samples Applied Biosystems sophisticated Detection Kit is part of a fully integrated, bioinformatics process to increase single-vendor solution that includes the assay’s reliability in meeting the Applied Biosystems real-time PCR following criteria: systems, software and reagents designed to deliver superior speed, • DNA of the target organism yields a simplicity and accuracy. positive signal 2. Prepare samples TaqMan Pathogen Detection Kits are • DNA of closely related organisms available for the following organisms: yields a negative signal • Cronobacter sakazakii 1 • Negative controls yield a negative (p/n 4382492) signal • Salmonella enterica 2 Validation for specific applications of the 3. Run samples Certified by AOAC and AFNOR TaqMan Cronobacter sakazakii Detection (p/n 4366104) Kit must be performed in your laboratory according to your laboratory’s validation • Campylobacter jejuni 2 procedures. (p/n 4366098)
Benefit from Unique Kit Format • E. coli O157:H7 2 The TaqMan assay’s easy-to-use, (p/n 4366100) closed-tube format minimizes the risk • Listeria monocytogenes 2 of contamination. Once samples and (p/n 4366102) Use DNA-Based Method reagents are added, the tubes remain 1 The TaqMan Cronobacter sakazakii closed throughout the amplification and • Pseudomonas aeruginosa Detection Kit uses PCR to amplify a DNA detection process. All TaqMan Pathogen (p/n 4368604)
Detection Kits are based on the same 1 target sequence unique to Cronobacter • Staphylococcus aureus sakazakii. Identification is made if, and standardized protocol. This enables you (p/n 4368606) only if, the target sequence is present. to run any combination of these assays 1 These kits have been verified through Applied The detection process can be performed together in the same sample run. Biosystems’ internal verification procedures. in less than two hours. 2 These kits have been validated as described in TaqMan ® Pathogen Detection Kits–Validation Reference White Paper (stock number 116WP01-01).
For environmental (including soil, air and water), quality control, and food and finished products testing, and for research use only. Not for use in diagnostic procedures. This product has not been cleared or otherwise approved by the United States Food and Drug Administration or by any other regulatory body in any country, or under the European IVD Directive, for diagnostic or any other clinical purpose. The purchaser or user of this product agrees not to use this product for diagnostic or other clinical purposes. © Copyright 2008, 2011 Applied Biosystems. All Rights Reserved. AB (Design), Applied Biosystems, and Applera are registered trademarks of Applied Biosystems or its subsidiaries in the U.S. and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Printed in the USA, 07/2011 Publication 116MI29-02
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