Applied Biosystems Food Pathogen Detection System
TaqMan ® E.coli O157:H7 Detection Kit
A simple, reliable, and rapid procedure
TaqMan® Pathogen Detection Kits
• E. coli O157:H7
• Listeria monocytogenes
• Pseudomonas aeruginosa
• Salmonella enterica
• Staphylococcus aureus
When Time is of the Essence A Proven Alternative Up to Four Times as Fast Escherichia coli O157:H7, one of over The TaqMan E. coli O157:H7 Detection Traditional E. coli O157:H7 detection 700 reported pathogenic serotypes Kit uses Real-Time PCR technology methods can take up to 7 days; of E. coli, is the causative agent of designed to provide: immunoassay methods can take up to hemorrhagic colitis. Culture-based 4 days. Time to result with the TaqMan • Faster time to results testing for E. coli O157:H7 is time- and E. coli O157:H7 Detection Kit is less than labor- intensive. Selective detection of • Excellent specificity and sensitivity 4 hours after pre-enrichment and less E. coli O157:H7 can also be difficult, than 20 hours total. • Exceptional ease-of-use because it is often found in lower abundance than other pathogens. • Reduced risk of contamination Exceptional Ease of Use • E. coli O157:H71 Results in three easy steps • Three easy steps (p/n 4366100)
1. Enrich samples • Software-guided assay procedure • Salmonella enterica1 Certified by AOAC and AFNOR • No electrophoresis or post-PCR (p/n 4366104) processing • Campylobacter jejuni 1 The TaqMan E. coli O157:H7 Detection (p/n 4366098) Kit gives you reliable results in just three simple steps. RapidFinder™ software • Cronobacter sakazakii 2 2. Prepare samples guides you through the assay procedure, (p/n 4382492) and minutes after detection is complete, • Listeria monocytogenes1 results are displayed on an easy-to-read (p/n 4366102) screen for fast, accurate interpretation. • Pseudomonas aeruginosa 2 Minimal Risk of Contamination (p/n 4368604) The TaqMan assay uses a closed-tube 3. Run samples format. Once samples and reagents • Staphylococcus aureus 2 are added, the tubes remain closed (p/n 4368606) throughout the amplification and 1 These kits have been validated as described in detection process, minimizing risk of TaqMan® Pathogen Detection Kits–Validation cross contamination. Reference White Paper (stock number 116WP01-01).
Preformatted and Ready to Use 2 These kits have been verified through Applied The TaqMan E. coli O157:H7 Detection Biosystems’ internal verification procedures. Kit includes reagents for preparation and These kits are based on universal assay amplification of 100 samples, including: Demonstrated Specificity conditions, enabling you to run any and Sensitivity • E. coli O157:H7 target assay mix combination of these assays together in The TaqMan E. coli O157:H7 Detection the same sample run. Kit has demonstrated: • Environmental master mix A Complete Solution • Negative control • 100% inclusivity for 16 strains of In combination with Applied Biosystems E. coli O157:H7 and 055:H7 Each reaction contains an Internal Real-Time PCR Systems such as the
™ • 100% exclusivity for 52 other E.coli Positive Control (IPC) that controls for 7300, 7500, 7500Fast and StepOne strains and non-E. coli strains the presence of inhibitors for reliable Systems, RapidFinder software, and negative results. the PrepMan® Ultra Sample Preparation • Detects down to 1 cfu in 25 grams of Reagent, the TaqMan E. coli O157:H7 enriched food sample Part of a Growing Family Detection Kit offers a fully-integrated, Applied Biosystems offers a full line of single-vendor solution that provides TaqMan Pathogen Detection kits: superior performance, reliability, and ease-of-use.
For environmental (including soil, air and water), quality control, and food and finished products testing, and for research use only. Not for use in diagnostic procedures. This product has not been cleared or otherwise approved by the United States Food and Drug Administration or by any other regulatory body in any country, or under the European IVD Directive, for diagnostic or any other clinical purpose. The purchaser or user of this product agrees not to use this product for diagnostic or other clinical purposes. NOTICE TO PURCHASER: LIMITED LICENSE. Use of the TaqMan® E. coli O157:H7 Pathogen Detection Kit (p/n 4366100) is covered by one or more of the following US patents and corresponding patent claims outside the US: 5,079,352, 6,127,155, 5,677,152 (claims 1-23 only), and 5,773,258 (claims 1 and 6 only), 5,210,015, 5,487,972, 5,804,375, 5,538,848, 5,723,591, 5,876,930, 6,030,787, 6,258,569, and claims outside the US corresponding to US Patent No. 4,889,818. The purchase of this product includes a limited, non-transferable immunity from suit under the foregoing patent claims for using only this amount of product solely in environmental testing, quality control/quality assurance testing and food testing applications including reporting results of purchaser’s activities for a fee or other commercial consideration, and also for the purchaser’s own internal research. No right under any other patent claim (such as apparatus or system claims in US Patent No. 6,814,934) is conveyed expressly, by implication, or by estoppel. Further information on purchasing licenses may be obtained from the Director of Licensing, Applied Biosystems, 850 Lincoln Centre Drive, Foster City, California 94404, USA. © Copyright 2008, Applied Biosystems. All Rights Reserved. Applera, Applied Biosystems, AB (Design), and PrepMan are registered trademarks and RapidFinder and StepOne are trademarks of Applera Corporation or its subsidiaries in the U.S. and/or certain other countries. TaqMan is a registered trademark of Roche Molecular Systems, Inc. Printed in the USA, 12/2008 Publication 116MI31-01
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