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Surfactant Film Coating Aid in Germiantion Under Drought Stress 7thNational Congress on Medicinal Plants 12-14th May 2018 Shiraz, Iran Poster Presentations Abstract book of National Congress on Medicinal Plants (NCMP2018) 12-14th May 2018 Shiraz, Iran 7thNational Congress on Medicinal Plants 12-14th May 2018 Shiraz, Iran 10 Essential Oil Composition, Phenolic and Total Phenolic Contents and Antioxidant Activity from Aerial Parts and Oleo-Gum-Resin of Ferula Assa foetida L. Leila Rowshandel,* Atefeh Bahmanzadegan, Faraneh Zareiyan, Vahid Rowshan Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, Shiraz, Iran. E-mail: [email protected] The present work was identified the chemical compounds of essential oil (EO), evaluated the antioxidative activities, phenolic and total phenolic contents (TPCs) from aerial parts of Ferula assa-foetida and its oleo-gum-resin. The EO components were analyzed by gas chromatography (GC-FID) and gas chromatography/mass spectrometry (GC/MS). Polyphenolic compounds of metanolic extract of its plant and its oleo-gum-resin were examined by using HPLC analysis. The TPC was assessed by Folin-Ciocalteu method. Furthermore, radical scavenging activity of sample was assessed using Diphenylpicrylhydrazyl (DPPH). Essential oil's analyses showed that 10-epi-ɣ-eudesmol( 18.3%), α-eudesmol (10.9%), agarospirol (7.1%), β-eudesmol (6.6%) and guaiol (5.8%) were the main components from F. assa-foetida aerial parts and α-pinene (22.6%), (Z)-1-propenyl sec-butyl disulfide (20.4%), β-pinene (12.1%), (E)-1- propenyl sec-butyl disulfide (6.6%) and bicyclogermacrene (4.1%) were the main components from its oleo-gum-resin. Chloregenic acid (2.6 mg/g), ellagic acid (1.3 mg/g), quercetin (0.18 mg/g) and vanillin (0.05 mg/g) were the main polyphenols detected in extract of plant. Also in its oleo-gum-resin were carvacrol (3.6 mg/g), trans-ferulic acid (2.01 mg/g), eugenol(1.4 mg/g), hesperetin (0.73 mg/g), hesperedin (0.66 mg/g) and vanillin (0.22 mg/g), respectively. The TPC from F. assa-foetida aerial part was 50.50 ± 0.74 mg/g and from oleo-gum-resin was 76.12 ± -1 6.52 mg/g. The result showed IC50 of 1463.99 ± 70.68µg ml in 517 nm from F. assa-foetida -1 aerial part and IC50 of 1417.23± 20.50 µg ml from oleo-gum-resin. Results implied that F. assa- foetida and its oleo-gum-resin are valuable sources of natural compounds with important biological properties. Keywords: Ferula assa-foetida, EO, Total phenol, Polyphenol, Antioxidant activity References [1] Hadavand Mirzaei, H.; Hasanloo, T. Res. J. Pharmacog. 2014. 1, 51-54. [2] Ahmadvand, H.; Amiri, H.; Elmi, Z.D.; Bagheri, S. Iranian. J. Pharmacol. Ther. 2013, 12, 52-57. [3] Bahrami, G.; Soltani, R.;Sajjadi, S. E.; Kanani, M. R.; Naderi, R.;Ghiasvand, N.; Shokoohinia, Y. J. Rep. Pharm. Sci. 2013, 2, 90-97. 7thNational Congress on Medicinal Plants 12-14th May 2018 Shiraz, Iran 11 Investigation of Chemical Composition of Essential oils, Phenolic and Total Phenolic Contents and Antioxidant Activity from Aerial Parts and Oleo-gum-resin of Ferula gummosa Boiss. Leila Rowshandel*, Atefeh Bahmanzadegan, Faraneh Zareiyan, Vahid Rowshan Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, Shiraz, Iran. E-mail: [email protected] The present work was identified the chemical compounds of essential oil (EO), evaluated the antioxidative activities, phenolic and total phenolic contents (TPCs) from aerial parts of Ferula gummosa and its oleo-gum-resin. The EO components were analyzed by gas chromatography (GC-FID) and gas chromatography/mass spectrometry (GC/MS). Polyphenolic compounds of metanolic extract of its plant and its oleo-gum-resin were examined by using HPLC analysis. The TPC was assessed by Folin-Ciocalteu method. Furthermore, radical scavenging activity of sample was assessed using Diphenylpicrylhydrazyl (DPPH). Essential oil's analyses showed that α-pinene (18.8%), Bulnesol (14.6%) and Humulene epoxide II (9.6%) were the main components from F. gummosa aerial parts and ß-pinene (34.2%), Bulnesol (5.5%) and Limonene (4.9%) were the main components from its oleo-gum-resin. Catechin (3.38 mg/g), chloregenic acid (0.70 mg/g), rosmarinic acid (0.20 mg/g), trans-ferulic acid (0.17 mg/g) and quercetin (0.16 mg/g) were the main polyphenols detected in extract of plant. Also in its oleo-gum-resin were eugenol (0.44 mg/g), trans-ferulic acid (0.07 mg/g), p-Coumaric (0.02 mg/g) and quercetin (0.02 mg/g), respectively. The TPC from F. gummosa aerial part was 5.08 ± 0.11 mg/g and from oleo- -1 gum-resin was 4.10 ± 0.03 mg/g. The result showed IC50 of 4008.24 ± 89.56µg ml in 517 nm -1 from F. gummosa aerial part and IC50 of 5698.75± 278.93 µg ml from oleo-gum-resin. Results implied that F. gummosa and its oleo-gum-resin are valuable sources of natural compounds with important biological properties [1,2]. Keywords: Ferula gummosa, EO, Total phenol, Polyphenol References [1] Hadavand Mirzaei, H.; Hasanloo, T. Res. J. Pharmacog. 2014. 1, 51-54. [2] Ahmadvand, H.; Amiri, H.; Elmi, Z.D.; Bagheri, S. Iranian. J. Pharmacol. Ther. 2013, 12, 52-57. [3] Bahrami, G.; Soltani, R.;Sajjadi, S. E.; Kanani, M. R.; Naderi, R.;Ghiasvand, N.; Shokoohinia, Y. J. Rep. Pharm. Sci. 2013, 2, 90-97. 7thNational Congress on Medicinal Plants 12-14th May 2018 Shiraz, Iran 12 Composition of the Essential Oils, Total phenolic Contents and Antioxidant Activity of Aerial Parts and Oleo-gum-resin of Ferula persica Willd. Leila Rowshandel* Atefeh Bahmanzadegan, Faraneh Zareiyan, Vahid Rowshan Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, Shiraz, Iran. E-mail: [email protected] The present work was identified the chemical compounds of essential oil (EO), evaluated the antioxidative activities, phenolic and total phenolic contents (TPCs) from aerial parts of Ferula persica and its oleo-gum-resin. The EO components were analyzed by gas chromatography (GC-FID) and gas chromatography/mass spectrometry (GC/MS). Polyphenolic compounds of metanolic extract of its plant and its oleo-gum-resin were examined by using HPLC analysis. The TPC was assessed by Folin-Ciocalteu method. Furthermore, radical scavenging activity of sample was assessed using Diphenylpicrylhydrazyl (DPPH). Essential oil's analyses showed that α-pinene (18.4%), Myrcene (16.3%) and α -Bisabolol (8.8%) were the main components from F. persica aerial parts and α-pinene (24.4%), (Z)-1-propenyl sec-butyl disulfide (18.3%) and ß-pinene (12.5%) were the main components from its oleo-gum-resin. Chloregenic acid (2.91 mg/g), catechin (0.89 mg/g), hesperetin (0.51 mg/g) and p-Coumaric acid (0.05 mg/g) were the main polyphenols detected in extract of plant. Also, trans-ferulic acid (1.54 mg/g) was in its oleo-gum-resin.The TPC from F. persica aerial part was 70.21 ± 0.32 mg/g and -1 from oleo-gum-resin was 46.85 ± 1.51 mg/g. The result showed IC50 of 1209.89 ± 20.97µg ml -1 in 517 nm from F. persica aerial part and IC50 of 1840.23± 94.34 µg ml from oleo-gum-resin. Results implied that F. persica and its oleo-gum-resin are valuable sources of natural compounds with important biological properties [1]. Keywords: Ferula persica, EO, Total phenol, Polyphenol, Antioxidant activity References [1] Hadavand Mirzaei, H.; Hasanloo, T. Res. J. Pharmacog. 2014. 1, 51-54. [2] Ahmadvand, H.; Amiri, H.; Elmi, Z.D.; Bagheri, S. Iranian. J. Pharmacol. Ther. 2013, 12, 52-57. 7thNational Congress on Medicinal Plants 12-14th May 2018 Shiraz, Iran 13 Phenolic and Total Phenolic Contents, Antioxidant Activity and Essential Oil Composition from Aerial Parts and Oleo-gum-resin of Dorema ammoniacum D. Don. Leila Rowshandel,* Atefeh Bahmanzadegan, Faraneh Zareiyan, Vahid Rowshan Department of Natural Resources, Fars Agricultural and Natural Resources Research and Education Center, Shiraz, Iran. E-mail: [email protected] The experiment was carried out using the shadow-dried aerial parts of Dorema ammoniacum D. Don. and its oleo-gum-resin in order to investigate the polyphenolic compositions, total phenolic contents (TPCs), antioxidant activity and essential oil (EO) analysis. Polyphenolic compounds of metanolic extract of its plant and its oleo-gum-resin were examined by using HPLC analysis. The TPC was assessed by Folin-Ciocalteu method. Furthermore, radical scavenging activity of sample was assessed using Diphenylpicrylhydrazyl (DPPH). The EO components were analyzed by gas chromatography (GC-FID) and gas chromatography/mass spectrometry (GC/MS). Chloregenic acid (2.1 mg/g), ellagic acid (1.2 mg/g), hesperedin (0.54 mg/g), eugenol (0.53 mg/g), hesperetin (0.40 mg/g), catechin (0.35 mg/g) and vanillin (0.20 mg/g) were the main polyphenols detected in extract of plant, respectively. Also in its oleo-gum- resin were rutin (1.02 mg/g), catechin (0.93 mg/g), carvacrol (0.58 mg/g), hesperedin (0.30 mg/g) and vanillin (~0.10 mg/g), respectively. The TPC from D. ammoniacum aerial part was 79.64 ± 3.25 mg/g mg/g and from oleo-gum-resin was 71.28 ± 0.43 mg/g. The result showed -1 IC50 of 659.28± 41.87µg ml in 517 nm from D. ammoniacum aerial part and IC50 1356.47± 21.03 µg ml-1 from oleo-gum-resin. Essential oil's analyses showed that tetradecanal (13.5%), geranyl acetone (9.6%), n-hexadecanal (8.5%), n-heptadecane (8.1%), (E)-dihydro-apofarnesol (7.5%) and (E)-nerolidol (6.7%) were the main components from D.
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