OriGene Technologies, Inc. OriGene Technologies GmbH

9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected]

AM20047PU-N Monoclonal Antibody to KDEL Receptor 1 / KDELR1 (C-term) - Purified

Alternate names: ER lumen retaining receptor 1, ER marker, ERD2.1, KDEL protein retention receptor 1, Putative MAPK-activating protein PM23 Quantity: 0.1 mg Concentration: 1.0 mg/ml Background: HSP 70 family comprises four highly conserved , HSP 70, HSC 70, GRP 75 and GRP 78, which serve a variety of roles. They act as molecular chaperones, facilitating the assembly of multi-protein complexes; participate in the translocation of polypeptides across cell membranes and to the nucleus; and aid in the proper folding of nascent polypeptide chains (1, 2). GRP 78 is localized in the endoplasmic reticulum (ER), where it receives imported secretory proteins and is involved in the folding and translocation of nascent peptide chains (2). Sorting of these proteins is dependent on a C-terminal tetrapeptide signal, usually KDEL in animal cells, and HDEL in S.cerevisiae (3). Uniprot ID: P24390 NCBI: NP_006792 GeneID: 10945 Host / Isotype: Mouse / IgG2b Recommended Isotype SM12P, AM03110PU-N Controls: Clone: 2E7 Immunogen: A synthetic HDEL peptide corresponding to the C-terminus of Yeast Bip. Format: State: Liquid purified IgG fraction Purification: Protein G Chromatography Buffer System: PBS, pH 7.4 containing 50% Glycerol with 0.09% Sodium Azide as preservative Applications: Western blot: 1/1000-1/2000. 1 µg/ml of this antibody was sufficient for detection of HDEL-containing proteins in 10 µg of S. cerevisiae lysate by colorimetric immunoblot analysis using Goat anti-mouse IgG -HRP as the secondary antibody. Immunofluorescence: 1/50-1/500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: Detects ~78kDa protein. The 2E7 clone recognizes the C-terminal peptide HDEL, a common version of the endoplasmic reticulum retention signal found in Yeast, Plant, nematode and other ER proteins. It specifically stains HDEL proteins in barnyard grass, beet, cotton, mung bean, sorghum and wheat (4).

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20160721 1 / 2 AM20047PU-N: Monoclonal Antibody to KDEL Receptor 1 / KDELR1 (C-term) - Purified

Species Reactivity: Tested: Drosophila, Yeast, Saccharomyce, Plants (barnyard grass, beet, cotton, mung bean, sorghum and wheat). Storage: Store undiluted at 2-8°C for one month or (in aliquots) at -20°C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Mayer M.P., and Bukau B. (2005) Cell Mol Life Sci. 62(6): 670-684. 2. Luo S., Mao C., Lee B., and Lee A.S. (2006) Mol Cell Biol. 26(15): 5688-5697. 3. : HDEL, Gene ID: 10945 4. Napier R.M., et al. (1992) J Cell Sci. 102: 261-271.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20160721 2 / 2

Powered by TCPDF (www.tcpdf.org)