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Table of Contents

How To Use This Catalog ...... 2 Service Information ...... 3-4 Placing Orders - Shipping...... 3 Miscellaneous Information...... 4 Product Information...... 5-17 Definitions ...... 5 Detergent Analysis ...... 6-7 Lipid-like Detergents...... 8-9 FOS-CHOLINE® Detergents ...... 8 FOS-MEA® ...... 9 Miscellaneous ...... 9 Nonionic Detergents ...... 10-14 Sugar-based Detergents ...... 10-11 Detergents Designed for Membrane Proteins...... 12 Polyoxyethylene Detergents ...... 13-14 Sherpas® - Polymeric Solubilization Aids ...... 15 Biochemicals ...... 16-17 MTS Reagents ...... 16 NDSB Reagents ...... 17 Books ...... 18 New Products ...... 19 Alphabetical Product Listing ...... 20-104 Kits ...... 105-111 Technical Data ...... 112-120 Detergent Solubility...... 112 Detergent Grades ...... 113 Detergent Stability ...... 114 Detergent Properties Tables...... 115-120 Indexes ...... 121-131 Catalog Number Index ...... 121-122 Chemical Name Index ...... 123-127 CAS Registry Number Index ...... 128-131

CATALOG Anatrace is a wholly owned subsidiary of USB Corporation. 2007-2008

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 1 How To Use This Catalog

Shipping and ordering information is given in the Service Information section on pages 3 and 4. Please note that we have changed our policy on shipping most deter- gents. Stability studies indicate that a cold pack is not necessary with most of the reagents we offer, including our high quality detergents (see page 114). For your convenience, we continue to ship most of our products by overnight service for our domestic customers and by air for our international customers.

The Product Information section on pages 5-17 gives data on both new and old products. Look in this section for general information on detergent and other product areas.

The New Product section on page 19 lists all the products that have been added since our previous catalaog edition.

To locate a particular detergent or other reagent, please check the Alphabetical Prod- uct Listing on pages 20-104 or use one of the three indexes available (Catalog Num- ber Index, pages 121-122; Chemical Name Index, pages 123-127 or CAS Registry Number Index, pages 128-131). Note that the Chemical Name Index lists a number of common names for many of our products. A search function is also available at www.anatrace.com.

Analytical procedures to determine Purity of our detergents (Detergent Analysis) are given on pages 6-7 and many detergent properties are listed in the Detergent Proper- ties Tables on pages 115-120.

For the latest product updates and additions, please visit our web site at www.anatrace.com.

2 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Service Information Placing Orders

Telephone: Monday-Friday 8:00 AM - 4:30 PM (EST) Toll free in the U.S. 800-252-1280 Or call 419-891-3030 Mail: Anatrace, Inc. 434 West Dussel Drive Maumee, OH 43537 Fax: 419-891-3037 Email: [email protected] Internet: www.anatrace.com Terms No minimum is required when ordering and all orders will be shipped promptly, usually on the same day. Net 30 days. Bulk Orders Should you need more than the largest size offered in the catalog, there can be considerable savings by ordering in bulk. When ordering please request special pricing. Prices Although we make every effort to keep prices constant, the prices herein may change without notice. Credit Cards We accept VISA®, MasterCard®, and American Express.

Shipping

Domestic Orders—Shipping and Handling—$25.00 All orders will be shipped by overnight service. Orders received before 1:00 PM EST will generally be shipped the same day.

International Orders—Shipping and Handling—$60.00 All orders will be shipped by express air. Our international customers are responsible for all duties and taxes at their location.

Please Note: Most detergents and other reagents offered by Anatrace have been shown to be stable under normal shipping conditions and will NOT be shipped with either an ice pack or dry ice. See Detergent Stability on page 114.

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 3 Service Information

Product Use Anatrace products are supplied for laboratory use only. They are to be used for laboratory research and experiments only. They may not be used for drugs, cosmetics, pesticide products or any use other than stated above. Package Weight Unless otherwise specified, the package will contain at least the indicated amount and usually slightly more. The user is cautioned to always measure the required amount from the container. Quality It is our goal to always supply the finest quality products available anywhere. If you are not completely satisfied that the chemical supplied to you meets the specifications described herein, we will replace it. Return Policy Should you receive a product ordered from this catalog which is believed to be nonconforming, please return the material within 30 days. You must receive authorization from Anatrace before returning the product. Returned products that have been ordered in error or have been retained more than 30 days are subject to a 25% restocking fee. Custom Synthesis Our research staff will be pleased to work with you on the synthesis and purification of a novel detergent or other reagent for your individual research needs. Custom Ampuling and Reagent Preparation Do you have a reagent that you must prepare frequently? Isn't it a waste of valuable lab time doing such routine work? We at Anatrace are experienced with a wide range of reagents and are accustomed to preparing reagents whether or not they contain detergents. We can custom blend or prepare reagents, or you can supply the reagent. So consider giving Anatrace the opportunity to prepare your reagents.

- Orders as small as 100 ampules - Volumes from 0.1 mL to 10 mL per ampul - Each container flushed with inert gases, as requested - Maximum container height of 85 mm - Flame sealed - Silk screen, transparent plastic, or opaque labels

Technical Support For detailed information and/or technical assistance call our toll free number during normal business hours: 800-252-1280. Inquiries can also be sent by Fax: 419-891-3037 or e-mail: [email protected].

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Definitions All concentrations listed as percent are weight/volume unless specifically stated. AGGREGATION NUMBER is the molecular weight of the micelle divided by the molecular weight of the detergent.

BSA is bovine serum albumin. PRODUCT INFORMATION CMC is the critical micelle concentration. [ ] is the Chemical Abstracts Registry Number (CAS Number). FW is the formula weight. PERCENT ALPHA is the percent alpha isomer measured by an HPLC method developed at Anatrace. μS is micro Siemens. FW avg. is the approximate, estimated FW for commercial detergents. NEW PRODUCTS

C-DODECAFOS, C-OCTAFOS, CYCLOFOS, CYGENT, CYPFOS, DIFOS, FOSFEN, and PMAL are trademarks of Anatrace, Inc. ANAGRADE, ANAMEG, ANAPOE, ANZERGENT, C-HEGA, CYGLU, CYMAL, FOS-CHOLINE, FOS-MEA, HEGA, SHERPA, and SOL-GRADE are registered trademarks of Anatrace, Inc. BRIJ is a registered trademark of ICI Americas. COOMASSIE is a registered trademark of Imperial Chemical Industries, PLC. EMPIGEN BB is a registered trademark of Albright & Willson. GENAPOL is a registered trademark of Hoechst AG. LUBROL is a registered trademark of Imperial Chemical, Inc. PLURONIC is a registered trademark of BASF Corporation. THESIT is a registered trademark of Desitin Arzneimittel GmbH. TRITON is a registered trademark of Union Carbide Chemicals & Plastics. TRYCOL is a registered trademark of Henkel Corporation. TWEEN is a registered trademark of ICI Americas. ZWITTERGENT is a registered trademark of Calbiochem-Novabiochem Corporation. INDEX

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Detergent Analysis At Anatrace, we prepare nearly all of the more than 100 detergents listed in our cata- log. Each lot is analyzed so that you the researcher can be assured of the highest consistent quality available anywhere. Nearly all of our ANAGRADE® detergents are purified chromatographically to be greater than 99% pure as measured by HPLC and to be low in UV absorbing or fluorescent impurities. We are pleased to list below the analytical procedures used to evaluate the detergents sold by Anatrace. Should you have any questions about these procedures, please feel free to contact us. Measurement of Purity (HPLC) ®

PRODUCT INFOMATION Nearly all detergents sold as ANAGRADE quality are greater than 99% pure as deter- mined by HPLC. SOL-GRADE® detergents are greater than 97% or 98% pure. The column used is a standard C18 column (4.6 mm x 250 mm) in conjunction with an RI detector. An eluant of either acetonitrile/water or /water is acceptable. The ratio will vary depending on the hydrophobicity of the detergent. Some examples are given below: DETERGENT Acetonitrile/water Methanol/water

NEW PRODUCTS n-Heptyl−β−D-glucopyranoside 25/75 55/45 n-Nonyl−β−D-glucopyranoside 35/65 55/45 n-Hexyl−β−D-maltopyranoside 20/80 40/60 n-Octyl−β−D-maltopyranoside 30/70 55/45 n-Nonyl−β−D-maltopyranoside 35/65 60/40 n-Dodecyl−β−D-maltopyranoside 45/55 75/25 n-Tridecyl−β−D-maltopyranoside 60/40 75/25 n-Hexadecyl−β−D-maltopyranoside 70/30 85/15

PRODUCT LISTING FOS-CHOLINE®-10 45/55 65/35 FOS-CHOLINE®-12 45/55 75/25 FOS-CHOLINE®-14 45/55 85/15 CYMAL®-3 35/65 75/45 CYMAL®-5 45/55 65/35 Some impurities may be less than one percent and still affect the properties of a detergent lot. Therefore, the following tests are also performed to insure that you receive the highest quality of detergent reagent available anywhere. Absorbance The absorbance of the detergent solution (1% w/v unless otherwise stated) in water is measured in the UV region. Glucopyranosides and maltopyranosides should have low absorbance throughout this region. Some detergents such as C-HEGA®, HEGA® and MEGA detergents have high absorbance at 225 nm. INDEX

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Fluorescence The fluorescence of the detergent solution is compared to a standard BSA solution

(0.1%) unless otherwise stated. The excitation wavelength is 280 nm and the emission PRODUCT INFORMATION is measured at 345 nm. A very low level of impurity can still result in a large fluores- cence background. Conductance For those detergents which are nonionic or zwitterions, a solution of detergent should have conductance nearly the same as deionized water. Measurement of conductance gives a very sensitive test for the presence of ionic impurities. Solubility in water Many of the impurities in detergent preparations are not soluble in water. Therefore

cloudiness of a detergent solution at a concentration where it is known to be soluble NEW PRODUCTS indicates the presence of an insoluble impurity. Measurement of pH The pH of the detergent solution at an appropriate concentration is measured. The pH should be neutral for detergents that are either uncharged or a zwitterion. contamination Glucoside and maltoside detergents are prepared from the corresponding hydrophobic alcohol. Trace amounts of this alcohol in the detergent lot can cause cloudiness in the detergent solution. Therefore, we measure the amount of alcohol in each lot of deter- gent. A 25 to 50% solution of detergent in methanol is injected into an HPLC system as described under Measurement of Purity (HPLC). The ratio of acetonitrile to water may vary depending on the hydrophobicity of the alcohol. Alpha isomer Glucoside and maltoside detergents have two isomeric forms - α and β. Each β detergent is analyzed for the percent a isomer present. A sample of detergent is injected into an HPLC system as described under Measurement of Purity (HPLC). For this analysis the ratio of acetonitrile to water is lower so that the detergent is retained longer and the separation of α and β isomers is accomplished. The ratio of solvents for separation will vary depending on the condition of the column and the detergent being analyzed. Lot analysis, shipping and storage Every lot of detergent sold by Anatrace will be shipped with an analysis sheet listing the results of the appropriate tests described above. All detergents are high purity products shipped to you by overnight delivery. ANAPOE® detergents should be stored refrigerated in the dark. All other detergents should be stored frozen and kept dry.

Warm to room temperature before opening the container. INDEX

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 7 Product Information Lipid-like Detergents Anatrace is pleased to offer more than twenty phospholipid analogs. Support for the development effort came from NIDDK through a Small Business Innovative Research (SBIR) grant (1R43DK49911-01, 2R44DK049911-02, and 5R44DK049911-03).

FOS-CHOLINE® detergents The detergents in this series have a phosphocholine headgroup, but unlike phospholipids they possess simple hydrophobic tails. n = 1, FOS-CHOLINE®-8 n = 2, FOS-CHOLINE®-9 O n = 3, FOS-CHOLINE®-10 O PO n = 4, FOS-CHOLINE®-11 n ® N O n = 5, FOS-CHOLINE -12 ® PRODUCT INFOMATION n = 6, FOS-CHOLINE -13 n = 7, FOS-CHOLINE®-14 n = 8, FOS-CHOLINE®-15 n = 9, FOS-CHOLINE®-16 The lack of a complex ester chain allows these detergents to be prepared and offered to the researcher at moderate prices, compared to many phospholipids. Note also that there are no chiral centers in these deter- gents as there are in the phospholipids and most phospholipid derivatives. FOS-CHOLINE®-12 (n-

NEW PRODUCTS dodecylphosphocholine) micelles have proven to be extremely useful as a medium in which to conduct NMR studies of membrane proteins1-4 and have also been shown to play a crucial role in refolding a misfolded mem- brane protein.5

FOS-CHOLINE®-ISO-9, FOS-CHOLINE®-ISO-11 To the phospholipid analog detergents discussed above, Anatrace has added FOS-CHOLINE®-ISO-9 and FOS- CHOLINE®-ISO-11 which possess a split hydrophobic tail packing more hydrophobicity into a shorter effective chain length. This detergent may be useful for the stabilization of proteins during crystallization. PRODUCT LISTING O O O PO O PO N O N O

FOS-CHOLINE®-UNSAT-11-10 FOS-CHOLINE®-UNSAT-11-10 contains an eleven carbon alkyl tail with a double bond at the end of the chain: O O PO N O INDEX

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FOS-MEA® detergents The FOS-MEA® detergents have a secondary amine in the head group instead of the quaternary amine found in the FOS-CHOLINE® detergents:

O

® PRODUCT INFORMATION O PO n = 1, FOS-MEA -8 H n = 3, FOS-MEA®-10 O n N n = 5, FOS-MEA®-12 H

Because of the replacement of the quaternary amine with a secondary amine, this series has a lower solubility than the FOS-CHOLINE® detergents.

™ CYCLOFOS detergents NEW PRODUCTS CYCLOFOS™ detergents combine the phosphocholine headgroup with an aliphatic tail containing a cyclohexyl group as present in the CYMAL® series of detergents.

n = 1, CYCLOFOS™-2 O n = 2, CYCLOFOS™-3 O PO n = 3, CYCLOFOS™-5 n N O n = 4, CYCLOFOS™-5 n = 5, CYCLOFOS™-6 n = 6, CYCLOFOS™-7

FOSFEN™-9 FOSFEN™-9 combines the phosphocholine headgroup with a phenyl ring-containing hydrophobic tail. O O PO

N O

Note: FOSFEN™-9 is prepared from a technical grade mixture of monoalkylphenols, predominantly para substituted. For more information see Merck Index 12,6775.

1. C. Fares, D. S. Libich, G. Harauz, FEBS J. 273, 601-614 (2006). 2. K. Oxenoid and J. J. Chou, Proc. Natl. Acad. Sci. USA 102, 10870-10875 (2005). 3. K. Choowongkomon, C. R. Carlin and F. D. Sonnichsen, J. Biol. Chem. 280, 24043-24052 (2005). 4. K. Oxenoid, F. D. Soennichsen and C. R. Sanders, Biochemistry 40, 5111-5118 (2001). 5. B. Gorzelle, J. K. Nagy, K. Oxenoid, W. L. Lonzer, et al, Biochemistry 38, 16373-16382 (1999). INDEX

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 9 Product Information Nonionic Detergents Most of the detergents useful in the extraction and purification of membrane proteins are nonionic detergents which possess either a sugar based or a polyoxyethylene head group.

Sugar-based detergents Sugar-based detergents are among the most popular type of detergents used with membrane proteins. The alkyl glucopyranoside series is available from the six carbon chain tail to dodecyl-β-D-glucopyranoside. The some- what less polar thioglycoside analogs of some of these compounds are also available.

n = 1, hexyl-β-D-glucopyranoside HO n = 2, heptyl-β-D-glucopyranoside HO O n = 3, octyl-β-D-glucopyranoside HO O n = 4, nonyl-β-D-glucopyranoside OH n n = 5, decyl-β-D-glucopyranoside PRODUCT INFOMATION n = 7, dodecyl-β-D-glucopyranoside

The high CMC value of hexyl-β-D-glucopyranoside (0.25M) indicates that a detergent with a shorter chain would typically not be useful. Decyl-β-D-glucopyranoside has a reasonable CMC value, but is marginally soluble. Dodecyl-β-D-glucopyranoside is even less soluble. For many membrane proteins, a member of the maltopyranoside series is milder and more effective than the

NEW PRODUCTS comparable member of the glucopyranoside series.

HO n = 1, hexyl-Β-D-maltopyranoside O n = 3, octyl-Β-D-maltopyranoside HO n = 4, nonyl-Β-D-maltopyranoside HO HO n = 5, decyl-Β-D-maltopyranoside OH O O n = 6, undecyl-Β-D-maltopyranoside HO O n = 7, dodecyl-Β-D-maltopyranoside n = 8, tridecyl- -D-maltopyranoside OH n Β n = 9, tetradecyl-Β-D-maltopyranoside n = 11, hexadecyl-Β-D-maltopyranoside PRODUCT LISTING Another advantage of the maltopyranoside head group is the increased solubility of the higher members of this series compared to the glucopyranoside series. Even hexadecyl-β-D-maltopyranoside is soluble although it must be heated to 50oC for a few minutes to make a “true” solution. The entire series of maltopyranoside detergents are available from hexyl-β-D-maltopyranoside to hexadecyl-β-D-maltopyranoside, except for the pentadecyl member. Since the maltopyranoside detergents are generally milder than the glucopyranoside detergents, one might think that maltotrioside detergents might be even more compatible with membrane proteins. Detergents with even larger head groups seem to be impractical because the higher polysaccharides are not available in pure form at a reasonable price. Two sugar-based detergents that have been available for some time are sucrose monododecanoate and sucrose monodecanoate. As with the maltose-based detergent series, these detergents are mild and useful for the extraction of membrane proteins. The researcher should be aware, however, that these detergents are esters rather than glycosidic compounds and thus are easily hydrolyzed in acid, base or by proteases. The sucrose INDEX ester detergents are also mixtures of isomers in which the aliphatic ester is linked to any one of the three primary alcohols in the sucrose structure.

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O

O HO O HO O HO HO O OH OH HO PRODUCT INFORMATION

Please note: Sucrose monoalkyl ester is a mixture of the three possible primary alcohol esters. Only one structure is shown here. The MEGA detergents are another series of glucose derivatives with an amide linkage between the alkyl tail and the glycoside head group:

O

N OH n = 1, MEGA-8 H NEW PRODUCTS OH n = 2, MEGA-9 H OH n = 3, MEGA-10 HO OH n H H

These detergents have been available for twenty years and have been used in numerous studies.1-6 As sugar- based detergents, they are rather easy to prepare and therefore reasonably priced. Unfortunately, their solubility is low, except for MEGA-8, and decreases as the alkyl chain is lengthened.

To overcome the low solubility, Anatrace has introduced a similar series: HEGA® detergents

OH O

N n = 1, HEGA®-8 OH ® H n = 2, HEGA -9 OH ® OH n = 3, HEGA -10 H ® HO OH n = 4, HEGA -11 n H H

The presence of an additional hydroxyl group results in increased solubility.

1. M. . Churchward, R. H. Butt, J. C. Lang, et al, Proteome Sci. 3, 5 (2005). INDEX 2. C. Roberts, B. Bond, I. R. White and H. J. Herdon, J. Recept. Signal Transduct Res. 20, 167-186 (2000). 3. D. Choudhury, A. Thompson, V. Stojanoff, et al, Science 285, 1061-1066 (1999). 4. W. H. Yue, Y. P. Zou, L. Yu and C. A. Yu, Biochemistry 30, 2303-2306 (1991). 5. D. M. Cortes and E. Perozo, Biochemistry 36, 10343-10352 (1997). 6. M. Hanatani, K. Nishifuji, M. Futai and T. Tsuchiya, J. Biochem. 95, 1349-1353 (1984).

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 11 Product Information Detergents Designed for Membrane Proteins CYMAL® Detergents1 Anatrace has developed more than two dozen detergents designed specifically for the extraction, purification and crystallization of membrane proteins. Support for the effort described here came from NIH through the Small Business Innovative Research (SBIR) program (1R43DK043172-01, 2R44DK043172-02, and 5R44DK043172-03). The first series made available was the CYMAL® detergents which have the formula:

HO

HO O HO HO OH O O HO O OH n PRODUCT INFOMATION The CMC values for these detergents are higher than for the corresponding linear chains containing the same number of carbons. Viewed another way, the cyclohexyl tails pack more hydrophobicity into a shorter effective chain length. Unpublished data from Dr. J. E. Harlan and Professor R. M. Garavito (Michigan State University) suggest that detergents that have a shorter chain length, but higher hydrophobicity, are useful for stabilizing proteins during crystallization. NEW PRODUCTS

CMC (mM) PRODUCT LISTING

Length (Angstroms) Number of Carbon Atoms r Cyclic Detergents n Aliphatic Detergents

A study published in late 1999 by the Dana Farber Cancer Institute and Harvard Medical School showed that from the patented CYMAL® group of detergents, CYMAL-5® may be the detergent of choice for separating and purifying G-protein coupled receptors.5

1. US Patent 5,674,987 and US Patent 5,763,586. 2. S. Askolin, J. P. Turkenburg, M. Tenkanen, et al, Acta Crystallogr. D Biol. Crystallogr. 60, 1903-1905 (2004). 3. H. Katayama, T. Tabata, Y. Ishihama, et al, Rapid Commun. Mass Spectrom. 18, 2388-2394 (2004). 4. G. J. Babcock, M. Farzan, and J. Sodroski, J. Biol. Chem. 278, 3378-3385 (2005). 5. Mirzabekov, T., Bannert, N., Farzan, M., Hofmann, W., Kolchisky, P., Wu, Lu., Ayalt, R. and Sodroski, I.,

INDEX J. of Biological Chemistry 274, 28745-28750 (1999).

12 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Information SERVICE INFORMATION Polyoxyethylene Glycol Detergents ANAPOE® Detergents Most polyoxyethylene detergents used in the early studies of membrane proteins were developed years ago for large industrial applications. Today an increasing number of well defined, highly puri- fied detergents, designed specifically for the extraction of membrane proteins in their active state are PRODUCT INFORMATION available. Detergents such as dodecyl−β−D-maltopyranoside and octyl−β−D-glucopyranoside have replaced many polyoxyethylene detergents. Yet for some proteins the polyoxyethylene detergents remain very useful—particularly for routine extraction procedures. Polyoxyethylene detergents are available in a variety of structures and under an often confusing list of trade names, such as TRITON®, TWEEN®, GENAPOL®, BRIJ®, THESIT®, LUBROL®, etc. In addition to the confusion caused by trade names, industrial detergents are often a mixture of closely related deter- gents that may vary from lot to lot. They may also contain additives and contaminants which result in undesirable effects during protein extraction. One such contaminant is an oxidizing agent.1 These

oxidants can increase in concentration upon aging. NEW PRODUCTS The “aging” of polyoxyethylene detergents results from the tendency of ethers to react with oxygen to form peroxides. Light accelerates this process.2-4 In samples of polyoxyethylene detergents, hydrogen peroxide and organic peroxides with a variety of structures may be found.2-4 The concentration of hydrogen peroxide equivalent can be as high as 0.2%.5 Conversely, it may be found that the level of peroxides has not increased over time because as they form they react with more detergent molecules, resulting in the presence of several undesirable derivatives.2 The presence of peroxides during extraction of membrane proteins has resulted in inactivation, activation and even fragmentation of biological materials.4 Sulfhydryl groups are readily oxidized by peroxides and such oxidation induces aggregation of acetylcholine receptors.6 Adding to the com- plexity, sulfhydryl oxidation is implicated in the transition from a conformation which has a high affinity for acetylcholine to a low affinity form. This transition has been noted during the purification process.7,8 Interference in biochemical analysis can also be caused by peroxides in detergent solutions. Lever has reported that the Kober reagent, used to measure estrogen, reacted with detergent solutions contain- ing peroxides to give brown solutions even without heating.2 Peroxides are probably responsible for the high blanks noted by Heath and Tappel when measuring lipid peroxides in detergent-solubilized membrane components.9 Even protein determinations can be affected, as noted by Stutzenberger for COOMASSIE® blue dye-binding assay, bicinchoninic acid method and the Folin phenol method.10

1. Chang, H. W. and Bock, E., Anal. Biochem. 104, (1980), 112-117. 2. Lever, M., Anal. Biochem. 83, (1977), 274-284. 3. Miki, T. and Orii, Y., Anal. Biochem. 146, (1985), 28-34. 4. Jaeger, J., Sorensen, K. and Wolff, J., Biochem. Biophys. Methods 29, (1994), 77-81. 5. Ashani, Y. and Catravas, G., Anal. Biochem. 109, (1980), 55-62. 6. Chang, H. W., Proc. Nat. Acad. Sci. USA 71, (1974), 2113-2117. 7. O’Brien, R. D. and Gibson, R. E., ABB 169, (1975), 458-463.

8. Chang, H. W. and Neumann, E., Proc. Nat. Acad. Sci. USA 73, (1976), 3364-3368. INDEX 9. Heath, R. L. and Tappel, A. L., Anal. Biochem. 76, (1976), 184-191. 10. Stutzenberger, F. J., Anal. Biochem. 207, (1992), 249-254.

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 13 Product Information

ANAPOE® detergents have been chromatographically purified to contain less than 20 μM of equivalent peroxide in a 10% solution. Each detergent is supplied in a 10% aqueous solution under argon gas in amber ampules.

Please note: ANAPOE® detergents are prepared from industrial detergents which are often mix- tures of closely related detergents. Even though ANAPOE® detergents are purified by chromatog- raphy, they are not necessarily homogenous. See ANAPOE® on pages 21-25 for pricing.

Cat. No. Description Cat. No. Description PRODUCT INFOMATION

® ® ® APT020 ANAPOE -20 (TWEEN 20) APO129 ANAPOE -C12E9 APB035 ANAPOE®-35 (BRIJ®-35) (Polyoxyethylene(9)dodecyl ether) ® APB058 ANAPOE®-58 (BRIJ®-58) AP1210 ANAPOE -C12E10 (Polyoxyethylene(10)dodecyl ether) APT080 ANAPOE®-80 (TWEEN® 80) APO138 ANAPOE®-C E APO106 ANAPOE®-C E 13 8 10 6 (Polyoxyethylene(8)tridecyl ether) (Polyoxyethylene(6)decyl ether) NEW PRODUCTS APND40 ANAPOE®-NID-P40 APO109 ANAPOE®-C E 10 9 ® ® (Polyoxyethylene(9)decyl ether) APX100 ANAPOE -X-100 (TRITON X-100) ® ® ® APX114 ANAPOE -X-114 (TRITON X-114) APO128 ANAPOE -C12E8 (Polyoxyethylene(8)dodecyl ether) APX305 ANAPOE®-X-305 (TRITON® X-305) APX405 ANAPOE®-X-405 (TRITON® X-405)

These ANAPOE® detergents are also available in kits: PRODUCT LISTING DSOL-ANAP1 ANAPOE® MASTER KIT (1 mL size) 30.50 Kit contains 1 mL of all 15 ANAPOE® detergents DSOL-ANP10 ANAPOE® MASTER KIT (10 mL size) 83.00 Kit contains 10 mL of all 15 ANAPOE® detergents

® DSOL-ANPC ANAPOE CyEx KIT (10 mL size) 51.50 ® ® ® Kit contains 10 mL of ANAPOE -35, ANAPOE -58, ANAPOE -C10E6, ® ® ® ANAPOE -C10E9, ANAPOE -C12E8, ANAPOE -C12E9, ® ® ANAPOE -C12E10, and ANAPOE -C13E8. DSOL-ANPT ANAPOE® TRITON/TWEEN KIT (10 mL size) 30.50 Kit contains 10 mL of ANAPOE®-X-100, ANAPOE®-X-114, ANAPOE®-X-305, ANAPOE®-X-405, ANAPOE®-20, and ANAPOE®-80. In each Kit detergents are supplied in a 10% aqueous solution under argon gas in amber ampules. INDEX

14 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Information SERVICE INFORMATION Sherpas® - Polymeric Solubilization Aids PMAL™-C12 was the first of a series of new polymeric reagents to be offered by Anatrace. PMAL™-C12 and the series that followed were first prepared and characterized under an NIH-SBIR grant (1R43GM060071-01, 2R44GM060071-02, 5R44GM060071-3) and are classified as amphipols. These polymers have a unique way of solubilizing an integral membrane protein.1-4 Amphipols wrap themselves around the hydrophobic transmem-

brane domains of membrane proteins so that the hydrophobic side chains of the polymer stabilize the hydropho- PRODUCT INFORMATION bic surface of the protein, while the polar side chains of the polymer confer aqueous solubility to the protein- amphipol complex. Recent papers describe the use of PMAL™-C12 for delivering complex integral membrane proteins (IMPs) into a membrane.5,6 Applications can be imagined where it would be desirable to add a purified membrane protein to lipid vesicles or biological membranes under conditions where the protein could spontaneously insert and adopt its functionally active state without lysing the target bilayer. These polymers appear to have some of the functions of the naturally occurring molecules called chaperones.7,8 Since they are synthetic polymers and not naturally occuring, the mechanism of transport may be different than chaperones. We are calling our synthetic transport aids Sherpas®, after the mountain people who transport items NEW PRODUCTS in difficult environments.

See pages 88-89 in the Product Listing section for information on the series of PMAL™ detergents. O O x

n x = 1, PMAL™-C8 ™ O NH x = 3, PMAL -C10 x = 5, PMAL™-C12 x = 9, PMAL™-C16 N

1. Tribet, C., Audebert, R. and Popot. J.-L., Proc. Nat. Acad. Sci 93, Amphipols: polymers that keep membrane proteins soluble in aqueous solution, 15047-15050 (1996). 2. Tribet, C., Audebert and R., Popot. J.-L., Langmuir 13, Stabilization of hydrophobic colloidal dispersions in water with amphiphilic polymers: application to integral membrane proteins, 5570-5576 (1997). 3. Tribert, C., Mills, D., Haiders, M. and Popot, J.-L., Biochemie 80, Scanning transmission electron microscopy study of the molecular mass of amphipol/cytochrome b6f complexes, 475-482 (1998). 4. Champeil, P., Menguy, T., Tribet, C., Popot, J.-L. and le Marie, M., J. Biol. Chem. 275, Interaction of amphipols with sarcoplasmic retitculum calcium Ca2+-ATPase, 18623-18637 (2000). 5. Nagy, J. K., Hoffmann, A. K., Keyes, M. H., Gray, D. N., Oxenoid K. and Sanders, C. R., FEBS Letters 501, Use of amphipathic polymers to deliver a membrane protein to lipid bilayers, 115-120 (2001). 6. Gorzelle, Bonnie M., Hoffmann, Amy Kuhn, Keyes, Melvin H., Gray, Don N., Ray, Dale G. and Sanders, Charles R., J. Am. Chem. Soc. 124, Amphipols Can Support the Activity of a Membrane Enzyme, 11594-11595 (2002). 7. Johnson, J. L. and Craig, E. A., Methods Enzymol. 351, Identifying functional interactions with molecular chaperones, 442-453 (2002). 8. Morello, J. P., Petaja-Repo, U. E., Bichet, D. G., and Bouvier, M., Trends Pharmacol. Sci. 21, Pharmacological chaperones: a new twist on receptor folding, 466-469 (2000). 9. Popot, J. L., Berry, E. A., Charvolin, D., Creuzenet, C., Ebel, C., Engelman, D. M., Flotenmeyer, M., Giusti, F., Gohon, Y., Hervi, P., Hong, Q., Lakey, J. H., Leonard, K., Shuman, H. A., Timmins, P., Warschawski, D. E., Zito, F., Zoonens, M., Pucci, B., Tribet, C., Cell. Mol. Life Sci. 60, Amphipols: polymeric surfactants for membrane biology research, 1559-1574 (2003). 10. Cuccia, L., Trends Biochem. Sci. 27, Amphipols: polymers that can keep membrane proteins active in aqueous solution, 550-550 (2002). INDEX 11. Martinez, K. L., Gohon, Y., Corringer, P. J., Tribet, C., Merola, F., Changeux, J. P., and Popot, J. L., FEBS Lett. 528, Allosteric transitions of Torpedo acetylcholine receptor in lipids, detergent and amphipols: molecular interactions vs. physical constraints, 251-256 (2002). 12. Prata, C., Giusti, F., Gohon, Y., Pucci, B., Popot, J. L., and Tribet, C., Biopolymers 56, Nonionic amphiphilic polymers derived from this (hydroxymethyl)acrylamidomethane keep membrane proteins soluble and native in the absence of detergent, 77-84 (2000).

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 15 Product Information Biochemicals Anatrace also offers several biochemicals including MTS reagents and NDSB reagents as well as convenience reagents to aid the researcher. All of our biochemicals are of the finest quality and can be ordered in bulk quantities. MTS Reagents The ability of MTS (methanethiosulfonate) reagents to react very rapidly and specifically with cysteine groups allows the quantitative derivatization of transmembrane proteins. The modification of the functional group attached to the methylthiosulfonate results in a variety of sulfhydryl specific reagents that can probe the struc- ture of a protein. For example, charged MTS reagents are soluble in water and insoluble in a hydrophobic environment. Reaction of charged MTS reagents indicates that the cysteine group is probably on or near the surface and not buried in the interior of the protein or membrane. Other MTS reagents contain a spin labeled or a fluorescent group to probe the protein/membrane environment.

PRODUCT INFOMATION Using site directed mutagenesis, one can introduce cysteine residues, one at a time, along a continuous stretch of residues. Modification of residues, particularly with charged MTS reagents, gives information about the accessibility of the residue in the ion channel. This technique was pioneered by Arthur Karlin and coworkers. SCAM, or substituted cysteine accessibility method, uses a small, polar, MTS reagent to modify each expressed mutant. The accessibility of the particular residue is inferred from the irreversible functional effects of these reagents. Anatrace is pleased to offer five charged MTS reagents: NEW PRODUCTS A110MT 2-AMINOETHYL METHANETHIOSULFONATE HYDROBROMIDE (MTSEA)

FW 236.15 C3H9NO2S2 HBr M110MT METHYL METHANETHIOSULFONATE (MMTS)

FW 126.20 [2949-92-0] C2H6O2S2 O110MT (1-OXYL-2,2,5,5-TETRAMETHYL-D3-PYRROLINE-3-METHYL) METHANETHIOSULFONATE (MTSL)

FW 264.30 [N/A] C10H18NO3S2 S110MT SODIUM (2-SULFONATOETHYL) METHANETHIOSULFONATE (MTSES)

FW 236.18 C3H7NaO5S3

PRODUCT LISTING T110MT [2-(TRIMETHYLAMMONIUM)ETHYL] METHANE THIOSULFONATE BROMIDE (MTSET)

FW 278.24 C6H16BrNO2S2 For more information see the MTS reagents in the Alphabetical Product Listing section. 1. P. J. Britto, L. Knipling, P. McPhie, and J. Wolff, Biochem. J. 389, 549-558 (2005). 2. A. Diez-Sampedro, D. D. Loo, E. M. Wright, et al, Biochemistry 43, 13175-13184 (2004). 3. E. M. Hedin, K. Hult, O. G. Mouritsen, and P. Hoyrup, J. Biochem. Biophys. Methods 60, 117-138 (2004). 4. N. Naber, S. Rice, M. Matuska, R. D. Vale, et al, Biophys. J. 84, 3190-3196 (2003). 5. Zhang, H. and Karlin, A., Biochem. 36, 15856-15864 (1997). 6. Akabas, M. H., Stauffer, D. A., Xu, M. and Karlin, A., Science 258, 307-310 (1992). 7. Stauffer, D. A. and Karlin, A., Biochem. 33, 6840-6849 (1994). 8. Akabas, M. H., Kaufman, C., Archdeacon, P. and Karlin, A., Neuron 13, 919-927 (1994). 9. Kenyon, G. L. and Bruice, T. W. in Methods Enzymol. 47-Part E, 407-430 (1977).

The following MTS reagent kits are also available: MT399-SMTS 134.92

INDEX (Each kit contains 100 mg of MTSEA, MMTS, MTSES, and MTSET)

16 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Information SERVICE INFORMATION

MT399-LMTS 538.08 (Each kit contains 500 mg of MTSEA, MMTS, MTSES, and MTSET) NDSB Reagents The series of NDSBs has been found to aid in the solubilization of some proteins. All members of the series, PRODUCT INFORMATION which are zwitterionic over a pH range of 3-12 and are very water soluble, have the unique ability to reduce protein aggregation while stabilizing protein structure. Other applications include refolding and renaturation of denatured proteins, isoelectrofocusing, and capillary electrophoresis. In addition, NDSB compounds are of benefit in some protein crystallizations.

1. M. S. Willis, J. K. Hogan, P. Prabhakar, et al, Protein Sci. 14, 1818-1826 (2005). 2. N. Expert-Bezancon, T. Rabilloud, L. Vuillard, and M. E. Goldberg, Biophys. Chem. 100, 469-479 (2003). 3. Vuillard, L., Baalbaki, B., Lehmann, M., Norager, S., Legrand, P. and Roth, M., Journal of Crystal Growth 168, 150-154 (1996). 4. Vuillard, L., et al, Biochem. J. 305, 337 (1995).

Anatrace is pleased to offer five NDSB reagents: NEW PRODUCTS ND195 NDSB-195 (Dimethylethylammoniumpropanesulfonate)

FW 195.3 C7H17NO3S ND201 NDSB-201 [(3-1-Pyridino)-1-propanesulfonate / 1-(3-Sulfopropyl)-pyridinium hydroxide, inner salt]

FW 201.2 C8H11NO3S ND211 NDSB-211 [Dimethyl(2-hydroxyethyl)ammonium-1-propanesulfonate, 98%]

FW 211.3 C7H17NO4S ND221 NDSB-221 [3-(1-Methylpiperidinium)-1-propanesulfonate]

FW 221.3 C9H19NO3S ND256 NDSB-256 (Dimethylbenzylammoniumpropanesulfonate)

FW 257.4 C12H19NO3S For more information see the NDSB reagents in the alphabetical Product Listing section.

These NDSB reagents are also available in kits: ND399-1 (1g size) 41.45 (Each kit contains 1 g of NDSB-195, NDSB-201, NDSB-211, NDSB-221, and NDSB-256) ND399-5 (5g size) 181.13 (Each kit contains 5 g of NDSB-195, NDSB-201, NDSB-211, NDSB-221, and NDSB-256) INDEX

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 17 Books BOOKS Anatrace is pleased to offer the following texts to our researchers. You may order books separately or include them with your product order.

BOOK01 Methods and Results in Crystallization of Membrane Proteins 109.95 Edited by So Iwata Part I: Introduction Part II: Principles and Techniques in Membrane Protein Crystallization Part III: Examples of Successful Crystallization of Membrane Proteins Part IV: Crystallization Informatics of Membrane Proteins "Membrane protein crystallisation is still one of the hardest challenges in Structural Biology. This book...is written to assist the researchers attempting to crystallize their own membrane proteins." - from book Abstract (Introduction)

BOOK02 Membrane Protein Protocols: Expression, Purification, and Characterization 99.50 Edited by Barry S. Selinsky

PRODUCT INFOMATION A collection of key techniques for the study of receptors and transport proteins. The book provides examples of how different membrane proteins can be over-expressed in both prokaryotic and eukaryotic expression systems, how natural and overexpressed proteins can be solubilized from their host membranes, and how the solubilized protein can be purified in active form. Each protocol contains step-by-step instructions to ensure success, troubleshooting advice, lists of reagents, and tips on avoiding pitfalls.

BOOK03 Protein Structure and Function 49.95 Petsko and Ringe

NEW PRODUCTS This text introduces general principles of protein structure, folding, and function, then goes beyond these basics to tackle the conceptual basis of inferring structure and function from genomic sequence. Written for upper-level undergraduates and beginning graduate students, Protein Structure and Function will also be useful for working scientists needing an up-to-date introduction to the field. (Available in the U.S. and Canada only)

BOOK04 Structure & Mechanism in Protein Science: 119.00 A Guide to Enzyme Catalysis and Protein Folding Alan Fersht There is a new era of protein design, sparked by the convergence of protein folding and enzymology. PRODUCT LISTING This book is a depiction of the principles of protein structure, activity, and mechanism today. It takes a more general look at mechanisms in protein science, emphasizing the unity of concepts in folding and catalysis and the importance of the relationships between basic chemistry, kinetics, thermodynamics, and structure. It makes protein engineering easier to understand and apply.

BOOK05 Proteins: Structures and Molecular Properties 102.00 T. E. Creighton In one convenient resource, Creighton’s landmark textbook offers an expert introduction to all aspects of proteins—biosynthesis, evolution, structures, dynamics, ligand binding, and catalysis. It works equally well as a reference or classroom textbook.

BOOK06 Introduction to Protein Structure 70.00 Branden and Tooze An up-to-date account of the principles of protein structure, with examples of key proteins in their biological context generously illustrated in full color. Introduces the general principles of protein structure and provides specific examples of proteins to show how they fulfil a wide variety of biological functions. Presents experimental approaches to determining and predicting protein structure, as well as engineering new proteins to modify their functions. INDEX

BKPKG1 Any Three Books 10% Discount

18 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com New Products SERVICE INFORMATION NEW PRODUCTS

The following new Detergents are listed in this catalog: PRODUCT INFORMATION Detergent Catalog # Page #

ANAPOE®-NID-P40 APND40 24 NONIDET P40 SUBSTITUTE NIDP40 77

The following new Reagents are listed in this catalog:

Reagent Catalog # Page # NEW PRODUCTS

AMPICILLIN, SODIUM SALT A1000 20 DTNB D1000 56 DTE D2001 55 EDTA E1000 57 EGTA E1001 57 NEM E2000 57 GUANIDINE HYDROCHLORIDE G1000 67 IPTG (PLANT), ANALYTICAL GRADE I1003P 73 LB BROTH, READY-MADE POWDER L1000 73 LITHUM DODECYL SULFATE (LDS) L1002 74 LURIA BROTH, READY-MADE POWDER L1001 74 MMTS M110MT 76 MTSL O110MT 76 TCEP T1000 93 TWEEN® 40 T1005 98 TWEEN® 60 T1006 99 UREA U1000 103 XGAL-IPTG B2050 103 INDEX

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 19 Product Listing

Catalog No. Product Name/Specifications Size Price

A110MT 2-AMINOETHYL METHANE THIOSULFONATE HYDROBROMIDE 100 mg 33.55 [MTSEA] 500 mg 134.20 1 g 241.56 • FW 236.15 [16599-33-0] C3H9NO2S2 HBr Solubility in water at 0-5°C ≥ 10% O Positively charged reagent that reacts very rapidly S S and specifically with cysteine groups. NH2 O

Half-life (pH 7.0, 20°C) ~ 12.0 minutes2 Half-life (pH 6.0, 20°C) ~ 92.0 minutes2 Half-life (pH 7.0, 4°C) ~ 116.0 minutes2

1. Karlin, A. and Akabas, M. H., in Methods Enzymol. 293, 123-136 (1998). 2. Sobszak, I. and Lolkema, J. S., Biochem. 42, 9789-9796 (2003). PRODUCT INFOMATION

A1000 AMPICILLIN, SODIUM SALT 5 g 15.00 25 g 41.00 FW 371.39 [69-52-3] C H N NaO SH H 100 g 127.00 16 18 3 4 N Form: White Powder S pH (10 mg/mL): 8.0-10.0 O N Water: < 2.0% O Methylene Chloride: < 0.2% O

NEW PRODUCTS NaO

A340 ANAMEG®-7, ANAGRADE® 1 g 36.93 [Methyl-6-O-(N-heptylcarbamoyl)−α−D-glucopyranoside / HECAMEG / 5 g 147.72 6-O-(N-Heptylcarbamoyl)-methyl−α−D-glucopyranoside] 25 g 590.89

O FW 335.4 [115457-83-5] C15H29NO7 CMC (H O) ~ 19.5 mM1 (0.65%) 2 O N Aggregation Number (H2O) ~ 92 H O HO

PRODUCT LISTING Purity ≥ 98% pure by HPLC analysis. HO pH (1% solution) 5-9 OH Solubility in water at 0-5°C ≥ 10% OCH3 Conductance (10% solution) < 80 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.03 280 nm < 0.05 260 nm < 0.07 225 nm < 0.1 1. Plusquellec, D., Chevalier, G., Talibart, R. and Wroblewski, H., Anal. Biochem. 179, 145-153 (1989).

ANAPOE detergents are purified industrial detergents.

INDEX Several homogenous polyoxyethylene detergents are available:

C8E4 (pg. 95), C8E5 (pg. 87), C8E6 (pg. 71), C10E5 (pg. 87), C10E6 (pg. 71), C12E8 (pg. 81).

20 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Listing SERVICE INFORMATION

Catalog No. Product Name/Specifications Size Price

APT020 ANAPOE®-20 50 ml (50 ampules/1 ml each) 67.06 [TWEEN® 20 / Polyoxyethylene(20)sorbitan monolaurate / 50 ml (5 ampules/10 ml each) 55.88 Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30 FW avg. 1228 [9005-64-5] 1 CMC (H2O) ~ 0.059 mM (0.0072%) PRODUCT INFORMATION HO(H2CH2CO)w (OCH2CH2)xOH

(OCH CH ) OH O 2 2 y

O(CH2CH2O)zR

OXIDANT-FREE Sum of w+x+y+z=20 R=C H C(O) Purified industrial detergent—may contain several components. 11 23 Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas.

1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). NEW PRODUCTS

APB035 ANAPOE®-35 50 ml (50 ampules/1 ml each) 67.06 ® [BRIJ -35 / C12E23 / a-Dodecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl / 50 ml (5 ampules/10 ml each) 55.88 Polyethylene glycol (23) monododecyl ether] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 1198 [9002-92-0] (C2H4O)nC12H26O, n ~ 23 1 CMC (H2O) ~ 0.091 mM (0.011%) 2 Aggregation Number (H2O) ~ 40 CH (CH ) O(CH CH O) H OXIDANT-FREE 3 2 11 2 2 23 Purified industrial detergent—may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. le Maire, M., Champeil, P. and Moller, J. V., Biochim. Biophys. Acta 1508, 86-111 (2000).

APB058 ANAPOE®-58 50 ml (50 ampules/1 ml each) 67.06 ® [BRIJ -58 / C16E20 / a-Hexadecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl / 50 ml (5 ampules/10 ml each) 55.88 Polyethylene glycol (20) monohexadecyl ether] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 1122 [9004-95-9] (C2H4O)nC16H34O, n ~ 20 1 CMC (H2O) ~ 0.004 mM (0.00045%)

OXIDANT-FREE CH3(CH2)15O(CH2CH2O)20H Purified industrial detergent—may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas.

1. For C16E21 listed in Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). INDEX

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 21 Product Listing

Catalog No. Product Name/Specifications Size Price

APT080 ANAPOE®-80 50 ml (50 ampules/1 ml each) 67.06 [TWEEN® 80 / Polyoxyethylene(80)sorbitan monolaurate / 50 ml (5 ampules/10 ml each) 55.88 Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan mono-9-octadecanoate] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30 FW avg. 1310 [9005-65-6] 1 CMC (H2O) ~ 0.012 mM (0.0016%) Aggregation Number (H O)2 ~ 58 HO(H2CH2CO)w 2 (OCH2CH2)xOH

(OCH CH ) OH O 2 2 y

O(CH2CH2O)zR

Sum of w+x+y+z=20 R=C H C(O) OXIDANT-FREE 17 33

PRODUCT INFOMATION Purified industrial detergent—may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html

® APO106 ANAPOE -C10E6 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(6)decyl ether / 50 ml (5 ampules/10 ml each) 55.88

NEW PRODUCTS 3,6,9,12,15,18-hexaoxaoctacosan-1-ol] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 423 [5168-89-8] (C2H4O)nC10H22O, n ~ 6 1 CMC (H2O) ~ 0.9 mM (0.038%) 2 Aggregation Number (H2O) ~ 40

OXIDANT-FREE CH3(CH2)9O(CH2CH2O)6H

Purified industrial detergent—C10E6 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas.

PRODUCT LISTING 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html

® APO109 ANAPOE -C10E9 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(9)decyl ether / 50 ml (5 ampules/10 ml each) 55.88 α-Decyl-ω-hydroxy-poly(oxy-1,2-ethanediyl] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 555 [26183-52-81] (C2H4O)nC10H22O, n ~ 9 1 CMC (H2O) ~ 1.3 mM (0.072%)

OXIDANT-FREE CH3(CH2)9O(CH2CH2O)9H

Purified industrial detergent—C10E9 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Borchardt, J. K., Lab Products Notebook 7, No. 10, 20 (1996). INDEX

22 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Listing SERVICE INFORMATION

Catalog No. Product Name/Specifications Size Price

® APO128 ANAPOE -C12E8 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(8)dodecyl ether / 50 ml (5 ampules/10 ml each) 55.88 3,6,9,12,15,18,21,24-octaoxahexatriacontan-1-ol] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 539 [3055-98-9] (C2H4O)nC12H26O, n ~ 8 1,2 CMC (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 0.09 mM (0.0048%) 3 PRODUCT INFORMATION Aggregation Number (H2O) ~ 123

OXIDANT-FREE CH3(CH2)11O(CH2CH2O)8H

Purified industrial detergent—C12E8 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. LeMaire, M., Kwee, S., Andersen, J. P., and Miller, J. V. Eur. J. Biochem., 129, 525-532, (1983). 2. LeMaire, M., Champeil, P., and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). 3. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html

® APO129 ANAPOE -C12E9 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(9)dodecyl ether / Thesit® 50 ml (5 ampules/10 ml each) 55.88 NEW PRODUCTS α-Dodecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 583 [3055-99-0] (C2H4O)nC12H26O n ~ 9 1 CMC (H2O) ~ 0.05 mM (0.003%)

OXIDANT-FREE CH3(CH2)11O(CH2CH2O)9H

Purified industrial detergent—C12E9 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% solution under argon gas. 1. Mast, R. C., and Haynes, L. V., J. Colloid Inerface Sci. 53, 35 (1975). 2. Rigler, P., Ulrich, W-P., Hovius, R., Ilegens, E., Pick, H., and Voegl, H., Biochemistry 42, 14017-14022 (2003).

® AP1210 ANAPOE -C12E10 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(10)dodecyl ether / 50 ml (5 ampules/10 ml each) 55.88 3,6,9,12,15,18,24,27,30-decaoxadotetracontan-1-ol] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 627 [6540-99-4] (C2H4O)nC12H26O, n ~ 10 1 CMC (H2O) ~ 0.2 mM (0.013%)

OXIDANT-FREE CH3(CH2)11O(CH2CH2O)10H

Purified industrial detergent—C12E10 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Mukerjee, P. and Mysels, K. J., NSRDS-NBS 36 (1970).

® APO138 ANAPOE -C13E8 50 ml (50 ampules/1 ml each) 67.06 [Polyoxyethylene(8)tridecyl ether] 50 ml (5 ampules/10 ml each) 55.88 100 ml (10 ampules/10 ml each) 100.58

FW avg. 553 [9043-30-5] (C2H4O)nC13H28O, n ~ 8 500 ml (screw cap bottle) 403.30 CMC (H O) ~ 0.1 mM1 (0.0055%) 2 INDEX

OXIDANT-FREE CH3(CH2)12O(CH2CH2O)8H

Purified industrial detergent—C13E8 is the major component. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Borchardt, J. K., Lab Products Notebook 7, No. 10, 20 (1996). 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 23 Product Listing

Catalog No. Product Name/Specifications Size Price

APND40 ANAPOE®-NID-P40 50 ml (50 ampules/1 ml each) 67.06 (Igepal CA-630 / [Octylphenoxy]polyethoxyethanol) 50 ml (5 ampules/10 ml each) 55.88 Nonidet P40 Substitute. 100 ml (10 ampules/10 ml each) 100.58 Chemically indistinguishable from Nonidet P40, which is 500 ml (screw cap bottle) 403.30 no longer commercially available. FW avg. 603 [2497-59-8] CMC (50mM Na+) ~ 0.05-0.3 mM1 1 Aggregation Number (H2O) ~ 100-155 OXIDANT-FREE Purified industrial detergent—may contain several components. (OCH2CH2)9OH Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html PRODUCT INFOMATION APX100 ANAPOE®-X-100 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-100 / 50 ml (5 ampules/10 ml each) 55.88 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- 100 ml (10 ampules/10 ml each) 100.58 poly(oxy-1,2-ethanediyl)] 500 ml (screw cap bottle) 403.30

FW avg. 647 [9002-93-1] t-Oct-C6H4-(OCH2CH2)XOH, x = 9-10 1-4 CMC (H2O) ~ 0.23 mM (0.015%) (w/v) 5 NEW PRODUCTS Aggregation Number (H2O) ~ 75-165 OXIDANT-FREE Purified industrial detergent— (OCH2CH2)10OH may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Vendittis, E., Paumbo, G., Parlata, G. and Borchini, U., Anal. Biochem. 115, 278-286 (1981). 2. Ross, S. and Oliver, J. P., J. Phys. Chem. 63, 1671-1674 (1959). 3. Mankovich, A. M., J. Amer. Oil Chem. Soc. 41, 449-452 (1964). 4. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983). PRODUCT LISTING 5. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).

APX114 ANAPOE®-X-114 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-114 / 50 ml (5 ampules/10 ml each) 55.88 α-[(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- 100 ml (10 ampules/10 ml each) 100.58 poly(oxy-1,2-ethanediyl)] 500 ml (screw cap bottle) 403.30

FW avg. 536 [9036-19-5] t-Oct-C6H4-(OCH2CH2)nOH, n ~ 7-8 1 CMC (H2O) ~ 0.2 mM (0.011%) (w/v) OXIDANT-FREE Purified industrial detergent— (OCH2CH2)8OH may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983). INDEX

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Catalog No. Product Name/Specifications Size Price

APX305 ANAPOE®-X-305 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-305 / α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]- 50 ml (5 ampules/10 ml each) 55.88 ω-hydroxy-poly(oxy-1,2-ethanediyl)] 100 ml (10 ampules/10 ml each) 100.58 500 ml (screw cap bottle) 403.30

FW avg. 1526 [9002-93-1] t-Oct-C6H4-(OCH2CH2)nOH, n ~ 30

OXIDANT-FREE PRODUCT INFORMATION Purified industrial detergent— may contain several components. Contains < 20 μM of equivalent peroxide. (OCH CH ) OH Supplied in a 10% (w/v) solution under argon gas. 2 2 30

APX405 ANAPOE®-X-405 50 ml (50 ampules/1 ml each) 67.06 [TRITON® X-405 / 50 ml (5 ampules/10 ml each) 55.88 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- 100 ml (10 ampules/10 ml each) 100.58 poly(oxy-1,2-ethanediyl)] 500 ml (screw cap bottle) 403.30 NEW PRODUCTS

FW avg. 1967 [9002-93-1] t-Oct-C6H4-(OCH2CH2)nOH, n ~ 40 1 CMC (H2O) ~ 0.81 mM (0.16%) OXIDANT-FREE Purified industrial detergent— (OCH2CH2)40OH may contain several components. Contains < 20 μM of equivalent peroxide. Supplied in a 10% (w/v) solution under argon gas. 1. McPherson, A., CSHL Press, Crystallization of Biological Macromolecules, (1999).

AZ308 ANZERGENT® 3-8, ANALYTICAL GRADE 5 g 24.13 [n-Octyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 N,N-dimethyl-N-(3-sulfopropyl)-1-octaminium hydroxide, inner salt] 100 g 308.88

FW 279.6 [15178-76-4] C13H29NO3S 1 CMC (H2O) ~ 390 mM (10.9%) O CH (CH ) N CH S O Purity ≥ 98% pure by HPLC analysis. 3 2 7 3 3 pH (1% solution) 5-8 O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 70 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1

ANZERGENT® 3-8 is chemically identical to ZWITTERGENT® 3-8. 1. Navarette, R. and Serrano, R., Biochim. Biophys. Acta 728, 403-408 (1983). INDEX

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Catalog No. Product Name/Specifications Size Price

AZ310 ANZERGENT® 3-10, ANALYTICAL GRADE 5 g 24.13 [n-Decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 N,N-dimethyl-N-(3-sulfopropyl)-1-decanaminium hydroxide, inner salt] 100 g 308.88

FW 307.6 [15163-36-7] C15H33NO3S 1 CMC (H2O) ~ 39 mM (1.2%) 2 Aggregation Number (H2O) ~ 41 O CH (CH ) N CH S O Purity ≥ 98% pure by HPLC analysis. 3 2 9 3 3 pH (1% solution) 5-8 O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 70 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 PRODUCT INFOMATION 260 nm < 0.06 225 nm < 0.1

ANZERGENT® 3-10 is chemically identical to ZWITTERGENT® 3-10. 1. Navarette, R. and Serrano, R., Biochim. Biophys. Acta 728, 403-408 (1983). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html

®

NEW PRODUCTS AZ312 ANZERGENT 3-12, ANALYTICAL GRADE 5 g 24.13 [n-Dodecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 N,N-dimethyl-1-N-(3-sulfopropyl)-1-dodecanaminium hydroxide, inner salt] 100 g 308.88

FW 335.5 [14933-08-5] C17H37NO3S CMC (20 mM Tris-HCl, pH 8.0, 0.1 M NaCl) ~ 2.8 mM1 (0.094%) 2 Aggregation Number (H2O) ~ 55-87 O CH (CH ) N CH S O Purity ≥ 98% pure by HPLC analysis. 3 2 11 3 3 pH (1% solution) 5-8 O

PRODUCT LISTING Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 70 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1

ANZERGENT® 3-12 is chemically identical to ZWITTERGENT® 3-12. 1. Vulliez-LeNormand, B. and Eisele, J. Anal. Biochem. 208, 241-243 (1993). 2. LeMaire, M., Champeil, P., and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).

The zwitterionic head group of the ANZERGENT® Series is the same

INDEX head group for NDSB-195 and NDSB-256 (pgs. 76 and 77).

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Catalog No. Product Name/Specifications Size Price

AZ314 ANZERGENT® 3-14, ANALYTICAL GRADE 5 g 24.13 [n-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate / 25 g 96.51 Dimethyl(3-sulfopropyl)tetradecyl-ammonium hydroxide, inner salt] 100 g 308.88

FW 363.6 [14933-09-6] C19H41NO3S 1 2 CMC (H2O) ~ 0.16 mM , CMC (10 mM Phosphate, pH 7.5) ~ 0.2 mM (0.007%) 3 Aggregation Number (H2O) ~ 83-130 O PRODUCT INFORMATION CH (CH ) N CH S O Purity ≥ 98% pure by HPLC analysis. 3 2 13 3 3 pH (1% solution) 5-8 O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 70 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 NEW PRODUCTS ANZERGENT® 3-14 is chemically identical to ZWITTERGENT® 3-14. 1. Anatrace measurement. 2. Brito, R. M. M. and Vaz, W. L. C. Anal. Biochem. 152, 250-255 (1986). 3. LeMaire, M., Champeil, P., and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).

AZ316 ANZERGENT® 3-16, ANALYTICAL GRADE 5 g 37.00 [n-Hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate] 25 g 129.00 100 g 369.00

FW 391.65 [2281-11-0] C21H45NO3S CMC 10-60 mM Aggregation Number 155 O CH (CH ) N CH S O Purity ≥ 98% pure by HPLC analysis. 3 2 15 3 3 pH (1% solution) 5-8 O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 70 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1

ANZERGENT® 3-16 is chemically identical to ZWITTERGENT® 3-16. INDEX

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Catalog No. Product Name/Specifications Size Price

AZ318 ANZERGENT® 3-18, ANALYTICAL GRADE 5 g 37.00 [n-Octadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate] 25 g 129.00 100 g 369.00

FW 419.71 [13177-41-8] C23H49NO3S Purity ≥ 98% pure by HPLC analysis. O pH (1% solution) 5-8 CH3(CH2)17 N CH3 S O Solubility in water at 20°C 30% 3 ≥ O Conductance (10% solution) < 70 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 PRODUCT INFOMATION ANZERGENT® 3-18 is chemically identical to ZWITTERGENT® 3-18.

B300 BIG CHAP, ANALYTICAL GRADE 1 g 67.60 [N,N'-bis-(3-D-Gluconamidopropyl)cholamide] 5 g 270.40 25 g 1081.60

FW 878.1 [86303-22-2] C42H75N3O16 1 O CMC (H2O) ~ 2.9 mM (0.25%) OH Aggregation Number (H O)1 ~ 10 O HO HO NEW PRODUCTS 2 N OH N Purity ≥ 95% pure by HPLC analysis. H OH O OH pH (1% solution) 5-8 OH HO OH N OH Solubility in water at 20°C ≥ 10% H HO OH Conductance (10% solution) < 100 μS HO Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.30 260 nm < 0.40

PRODUCT LISTING 1. Hjelmeland, L. M., Klee, W. A. and Osborne, J. C., Anal. Biochem. 130, 485-490 (1983).

B310 BIG CHAP, DEOXY, ANALYTICAL GRADE 1 g 171.60 [N,N'-bis-(3-D-Gluconamidopropyl)deoxycholamide] 5 g 686.40

FW 862.1 [86303-23-3] C H N O O 42 75 3 15 OH 1 CMC (H O) ~ 1.4 mM (0.12%) O HO HO 2 N Aggregation Number (H O)1 ~ 8-16 OH 2 N H OH O OH Purity ≥ 95% pure by HPLC analysis. OH HO N OH pH (1% solution) 5-8 H Solubility in water at 20°C > 10% HO OH HO Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.30

INDEX 260 nm < 0.40 1. Hjelmeland, L. M., Klee, W. A. and Osborne, J. C., Anal. Biochem. 130, 485-490 (1983).

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Catalog No. Product Name/Specifications Size Price

B2040 BLUE-GAL 50 mg 33.28 [5-Bromo-3-indolyl−β−D-galactopyranoside] 250 mg 124.80

FW 374.2 [97753-82-7] C H BrNO 14 16 6 H Purity ≥ 96% pure by HPLC analysis. N

HO PRODUCT INFORMATION 20 Specific Rotation [a] D (c=1, H2O:DMF 1:1) -34° ± 3° HO O Br Melting Point: 210-213°C HO O OH Solubility ≥ 2% in DMF IR spectrum conforms to specifications.

C640 C-DODECAFOS™, ANAGRADE® 1 g 135.51 [Cyclododecyl-1-phosphocholine] 5 g 542.06 25 g 2168.24

FW 349.17 [869549-38-2] C17H36NO4P NEW PRODUCTS 1 CMC (H2O) ~ 22 mM (0.77%) O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.

C630 C-OCTAFOS™, ANAGRADE® 1 g 135.51 [Cyclooctyl-1-phosphocholine] 5 g 542.06 25 g 2168.24

FW 293.13 [869550-29-8] C13H28NO4P 1 CMC (H2O) ~ 450 mM (13%) O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

CA100 CARBENICILLIN DISODIUM SALT 1 g 22.88 [α-Carboxybenzylpenicillin] 5 g 91.52 25 g 366.08 O ONa FW 422.4 [4800-94-6] C H N Na O S H H 17 16 2 2 6 N S IR spectrum conforms to specifications. O N Elemental analysis supplied with each lot. O O NaO

Carboxypenicillin antibiotic inhibits bacterial cell-wall synthesis (peptidoglycan cross-linking) by inactivating transpeptidases on the inner suface of the bacterial cell membrane. Can be used as a substitute for ampicillin at a similar concentration.

1. Perlman, D., Use of antibiotics in cell culture media, Meth. Enzymol., New York, NY (1979, Vol LVIII, 112. PRODUCT INFOMATION 2. Mandell, G., Antibacterial agents: Pencillins, cephalosporins, and other b-lactam antibiotics. The Pharmacological Basis of Therapeutics 9th ed., New York, NY (1996), 1074. 3. Raleigh, E.A., Escherichia coli, plasmids and bacteriophages, Short Protocols in Molecular Biology 2nd ed., New York, NY (1992), 1.

C316 CHAPS, ANAGRADE® 1 g 12.30 [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate / 5 g 49.18 NEW PRODUCTS N,N-Dimethyl-3-sulfo-N-[3-[[3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] 25 g 196.74 amino]propyl]-1-propanaminium hydroxide, inner salt]

FW 614.9 [75621-03-3] C32H58N2O7S 1 O CMC (H2O) ~ 8 mM (0.49%) OH 2 Aggregation Number (H2O) ~ 10 N H Purity ≥ 99% by HPLC analysis. N - pH (1% solution) 5-8 SO3 Solubility in water ≥ 0.5M HO OH Conductance (0.5 M solution) < 50 μS PRODUCT LISTING Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 1. Hjelmeland, L. M., Nebert, D. W. and Osborne, Jr., J. C., Anal. Biochem. 130, 72-82 (1983). 2. Womack, M. D., Kendall, D. A. and MacDonald, R. C., Biochim. Biophys. Acta 733, 210-215 (1983). 3. Bellis, S. L., Kass-Simon, G. and Rhoads, D. E., Biochem. 31, 9838-9843 (1992).

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price C316S CHAPS, SOL-GRADE® 5 g 30.74 [3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate / 25 g 122.96 N,N-Dimethyl-3-sulfo-N-[3-[[3a,5b,7a,12a)-3,7,12-trihydroxy-24-oxocholan-24-yl] 100 g 393.47 amino]propyl]-1-propanaminium hydroxide, inner salt]

FW 614.9 [75621-03-3] C32H58N2O7S 1 O PRODUCT INFORMATION CMC (H2O) ~ 8 mM (0.49%) OH 2 Aggregation Number (H2O) ~ 10 N H Purity ≥ 97% pure by HPLC analysis. N - pH (1% solution) 5-8 SO3 HO OH Solubility in water ≥ 0.5M Conductance (0.5 M solution) < 200 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.2 See C316 for references. NEW PRODUCTS

C317 CHAPSO, ANAGRADE® 1 g 34.98 [3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1-propanesulfonate] 5 g 139.92 25 g 559.68

FW 630.9 [82473-24-3] C32H58N2O8S 1 O CMC (H2O) ~ 8 mM (0.50%) OH Aggregation Number (H O)1 ~ 11 2 N H Purity ≥ 99% by HPLC analysis. N OH pH (1% solution) 5-8 - SO3 Solubility in water ≥ 0.5M HO OH Conductance (0.5M solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 1. Hjelmeland, L. M., Nebert, D. W. and Osborne, Jr., J. C., Anal. Biochem. 130, 72-82 (1983). 2. Cladera, J., Rigaud, J., Villaverde, J. and Dunach, M., Eur. J. Biochem. 243, 798-804 (1997). 3. Sanders, C. R. II and Prestegard, J. H., Biophys. J. 58, 447-460 (1990).

C408 C-HEGA®-8, ANAGRADE® 1 g 98.80 [Cyclohexylethanoyl-N-hydroxyethylglucamide] 5 g 370.50 25 g 1389.38

FW 349.5 [603111-75-7] C16H31NO7 1 CMC (H2O) ~ 277 mM (9.7%) OH O Purity ≥ 99% by HPLC analysis. N pH (1% solution) 5-8 OH H Solubility in water at 20°C ≥ 20% OH

Conductance (10% solution) < 100 μS H OH INDEX HO OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H Absorbance of a 1% detergent solution: 340 nm < 0.05 H 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 31 Product Listing

Catalog No. Product Name/Specifications Size Price

C409 C-HEGA®-9, ANAGRADE® 1 g 98.80 [Cyclohexylpropanoyl-N-hydroxyethylglucamide] 5 g 370.50 25 g 1389.38

FW 363.5 [864434-14-0] C17H33NO7 CMC (H O) ~ 108 mM1 (3.9%) 2 OH O

Purity ≥ 99% by HPLC analysis. N OH pH (1% solution) 5-8 H Solubility in water at 20°C ≥ 20% OH H OH Conductance (10% solution) < 100 μS HO OH H Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 PRODUCT INFOMATION 1. Anatrace measurement.

C410 C-HEGA®-10, ANAGRADE® 1 g 103.74 [Cyclohexylbutanoyl-N-hydroxyethylglucamide] 5 g 414.96 OH O 25 g 1659.84

FW 377.5 [864434-15-1] C18H35NO7 1 N CMC (H2O) ~ 35 mM (1.3%) OH H OH NEW PRODUCTS Purity ≥ 99% by HPLC analysis. H OH HO OH pH (1% solution) 5-8 H Solubility in water at 20°C ≥ 20% H Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. D. Choudhury, A. Thompson, V. Stojanoff, et al, Science 285, 1061-1066 (1999). PRODUCT LISTING

C411 C-HEGA®-11, ANAGRADE® 1 g 103.74 [Cyclohexylpentanoyl-N-hydroxyethylglucamide] 5 g 414.96 25 g 1659.84 OH O FW 391.5 [864434-16-2] C19H37NO7 1 CMC (H2O) ~ 11.5 mM (0.45%) N OH H Purity ≥ 99% by HPLC analysis. OH H OH pH (1% solution) 5-8 HO OH H Solubility in water at 20°C ≥ 10% H Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

CH200 CHOLESTEROL 50 g 19.76 [3β-Hydroxy-5-cholestene / 5-cholesten-3β-ol] 250 g 59.28 1 kg 177.84

FW 386.64 [57-88-5] C27H46O

Melting point: 147-150°C PRODUCT INFORMATION Loss in drying < 0.3% Residue on ignition < 0.1% Solubility in alcohol 1% HO IR spectrum conforms to specifications.

CH210 CHOLESTERYL HEMISUCCINATE TRIS SALT 1 g 21.18 5 g 84.74 . FW 607.9 [102601-49-0] C31H50O4 C4H11NO3 25 g 338.96

Melting Point: 202-206°C NEW PRODUCTS Appearance: White Powder Solubility (6% aqueous CHAPS): 1.2% O IR spectrum conforms to specifications. O NH O HO 3 O OH HO

Water soluble cholesterol standard: Klein, B., Kleinman, N. B. and Foreman, J. A., Clin. Chem. 20, 482-485 (1974).

1. Weiss, H. M. and Grisshammer, R., “Purification and characterization of the human adenosine A2a receptor functionally expressed in Escherichia coli”, Eur. J. Biochem. 269, 82-92 (2002). 2. Tucker, J. and Grisshammer, R., “Purification of a rat neurotensin receptor expressed in Escherichia coli”, Biochem. J. 317, 891-899 (1996). 3. Brown, P. J. and Schonbrunn, A., “Affinity Purification of a Somatostatin Receptor-G-protein Complex Demonstrates Specificity in Receptor-G-protein Coupling”, J. Biol. Chem. 268, No. 9, 6668-6676 (1993).

C3000 CMC DYE SOLUTION 10 ml (10 ampules/1 ml each) 30.16 [1mM aqueous solution of 8-Anilino-1-napthalene sulfonic acid / 50 ml (50 ampules/1 ml each) 120.64 8-(Phenylamino)-1-naphthalenesulfonic acid]

FW 299.35 [82-76-8] C16H13NO3S

NOTE: See Technical Bulletin #104 "CMC Determination by Dye Binding".

C6500 CULTURE TUBES 72 (6 x 50 mm) 25.99 - Borosilicate glass 720 (6 x 50 mm) 192.40 INDEX

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Catalog No. Product Name/Specifications Size Price

C508 CYCLOFOS™-2, ANAGRADE® 1 g 136.53 [2-Cyclohexyl-1-ethylphosphocholine] 5 g 546.12 25 g 2184.48 FW 293.8 [823796-65-2] C H NO P 13 28 4 O CMC (H O) ~ 256 mM1 (7.5%) 2 O PO Purity ≥ 98% pure by HPLC analysis. N O pH (1% solution) 5-9 Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION

C510 CYCLOFOS™-3, ANAGRADE® 1 g 136.53 [3-Cyclohexyl-1-propylphosphocholine] 5 g 546.12 25 g 2184.48

FW 306.91 [823796-66-3] C14H30NO4P 1 CMC (H2O) ~ 43 mM (1.3%) O Purity ≥ 98% pure by HPLC analysis. O PO

NEW PRODUCTS pH (1% solution) 5-9 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.

™ ®

PRODUCT LISTING C512 CYCLOFOS -4, ANAGRADE 1g 149.09 [4-Cyclohexyl-1-butylphosphocholine] 5 g 596.37 25 g 2385.45 FW 320.92 [675126-15-5] C H NO P 15 32 4 O CMC (H O) ~ 14 mM1 (0.45%) 2 O PO Purity ≥ 98% pure by HPLC analysis. N O pH (1% solution) 5-9 Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

C514 CYCLOFOS™-5, ANAGRADE® 1 g 149.09 [5-Cyclohexyl-1-pentylphosphocholine] 5 g 596.37 25 g 2385.45

FW 334.95 [657393-64-1] C16H34NO4P CMC (H O) ~ 4.5 mM (0.15%) 2 O Purity ≥ 98% pure by HPLC analysis. O PO PRODUCT INFORMATION pH (1% solution) 5-9 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1

C516 CYCLOFOS™-6, ANAGRADE® 1 g 171.60

[6-Cyclohexyl-1-hexylphosphocholine] 5 g 686.40 NEW PRODUCTS 25 g 2745.60

FW 349.17 [657393-65-2] C17H36NO4P 1 CMC (H2O) ~ 2.68 mM (0.094%) O O PO Purity 97% by HPLC analysis. ≥ N O pH (1% solution) 5-9 Solubility in water at 0-5oC ≥ 20%. Conductance (10% solution) < 500 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.

C518 CYCLOFOS™-7, ANAGRADE® 1 g 192.40 [7-Cyclohexyl-1-heptylphosphocholine] 5 g 769.60 25 g 3078.40

FW 363.3 [657393-66-3] C18H38NO4P 1 CMC (H2O) ~ 0.62 mM (0.022%) O Purity ≥ 97% pure by HPLC analysis. O PO pH (1% solution) 5-9 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 500 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

C702 CYGENT™ 1-3, ANAGRADE® 1 g 30.16 [1:1 mixture: N,N-Dimethyl-3(4-methyl-1-carboxy-2-cyclohexylamido) propylamine / 5 g 120.64 N,N-Dimethyl-3(4-methyl-2-carboxy-1-cyclohexylamido) propylamine] 25 g 482.56

FW 269.4 g/mol [869796-48-5] C14H25N2O3 O 1 CMC (H2O) > 500 mM N N Purity ≥ 99% by HPLC analysis. H pH (1% solution) 5-8 OH Solubility in water at 20°C ≥ 20% O Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 O Absorbance of a 1% detergent solution: 340 nm < 0.1 OH 280 nm < 0.3 H 260 nm < 0.5 N N

PRODUCT INFOMATION 1. Anatrace measurement. O

C323G CYGLU®-3, ANAGRADE® 1 g 70.70 [3-Cyclohexyl-1-propyl−β−D-glucoside] 5 g 264.59 25 g 994.21

FW 304.4 [869541-00-4] C15H28O6 NEW PRODUCTS 1 HO CMC (H2O) ~ 28 mM (0.86%) HO O Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 4 (HPLC) OH Percent cyclohexylpropanol < 0.005 (HPLC) pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 PRODUCT LISTING 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement.

Need to test several detergents? Check out our kits on pages 105-111.

INDEX If you require a custom kit, please request a quote.

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Catalog No. Product Name/Specifications Size Price

C324G CYGLU®-4, ANAGRADE® 1 g 89.98 [4-Cyclohexyl-1-butyl−β−D-glucoside] 5 g 337.43 25 g 1265.36 HO FW 318.4 [869542-54-1] C16H30O2 1 O CMC (H2O) ~ 1.8 mM (0.058%) HO

O PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. HO OH Percent alpha < 4 (HPLC) Percent (starting alcohol) < 0.005 (HPLC) pH (0.1% solution) 5-8 Solubility in water at 20°C > 0.1% Conductance (0.1% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06

225 nm < 0.08 NEW PRODUCTS 1. Anatrace measurement.

C321 CYMAL®-1, ANAGRADE® 1 g 89.98 [Cyclohexyl-methyl−β−D-maltoside1] 5 g 337.43 25 g 1265.36

FW 438.5 [26080-46-46] C19H34O11 2 2 CMC (H2O) ~ 340 mM (15%) CMC (0.15M NaCl) ~ 360 mM Purity ≥ 99% by HPLC analysis. HO

Percent alpha < 4 (HPLC) HO O Percent cyclohexylmethanol < 0.005 (HPLC) HO HO OH pH (1% solution) 5-8 O O Solubility in water at 20°C ≥ 20% HO O OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement.

Notes: INDEX

More information on the CYMAL series is available on page 12.

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Catalog No. Product Name/Specifications Size Price

C322 CYMAL®-2, ANAGRADE® 1 g 74.94 [2-Cyclohexyl-1-ethyl−β−D-maltoside1] 5 g 280.46 25 g 1053.86

FW 452.5 [260804-65-7] C20H36O11 2 2 CMC (H2O) ~ 120 mM (5.4%) CMC (0.15M NaCl) ~ 104 mM HO

Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 4 (HPLC) HO HO OH O O Percent cyclohexylethanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 OH Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 PRODUCT INFOMATION 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement.

C323 CYMAL®-3, ANAGRADE® 1 g 74.94 [3-Cyclohexyl-1-propyl−β−D-maltoside1] 5 g 280.46

NEW PRODUCTS 25 g 1053.86

FW 466.5 [181135-58-0] C21H38O11 2 2 CMC (H2O) ~ 34.5 mM (1.6%) CMC (0.15M NaCl) ~ 29 mM Aggregation Number (H O)3 ~ 38 2 HO

Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 4 (HPLC) HO HO OH Percent cyclohexylpropanol < 0.005 (HPLC) O O HO O pH (1% solution) 5-8 OH Solubility in water at 20°C ≥ 20%

PRODUCT LISTING Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Anatrace measurement. 3. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).

On-line ordering available at INDEX www.anatrace.com

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Catalog No. Product Name/Specifications Size Price

C324 CYMAL®-4, ANAGRADE® 1 g 89.98 [4-Cyclohexyl-1-butyl−β−D-maltoside1] 5 g 337.43 25 g 1265.36

FW 480.5 [181135-57-9] C22H40O11 2 2 CMC (H2O) ~ 7.6 mM (0.37%) CMC (0.15M NaCl) ~ 7.3 mM 2 Aggregation Number (H O) ~ 25 PRODUCT INFORMATION 2 HO

Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 4 (HPLC) HO HO OH Percent cyclohexylbutanol < 0.005 (HPLC) O O HO O pH (1% solution) 5-8 OH Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04

260 nm < 0.06 NEW PRODUCTS 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract.

C325 CYMAL®-5, ANAGRADE® 1 g 95.68 [5-Cyclohexyl-1-pentyl−β−D-maltoside1] 5 g 382.72 25 g 1530.88

FW 494.5 [250692-65-0] C23H42O11 2 2 CMC (H2O) ~ 2.4-5.0 mM (0.12%) CMC (0.15M NaCl) ~ 2.0 mM 2 Aggregation Number (H2O) ~ 47 HO Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 4 (HPLC) HO HO OH Percent cyclohexylpentanol < 0.005 (HPLC) O O pH (1% solution) 5-8 HO O Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract. 3. S. Askolin, J. P. Turkenburg, M. Tenkanen, et al, Acta Crystallogr. D Biol. Crystallogr. 60, 1903-1905 (2004). 4. H. Katayama, T. Tabata, Y. Ishihama, et al, Rapid Commun. Mass Spectrom. 18, 2388-2394 (2004). 5. D. Dorjsuren, Y. Badralmaa, J. Mikovits, et al, Protein Expr. Purif. 29, 42-50 (2003). 6. Wester, M. R., Johnson, E. F., Marques-Soares, C., Dansette, P. M., Mansuy, D., and Stout, C. D., Biochemisry 42, 6370-6379 (2003). INDEX

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Catalog No. Product Name/Specifications Size Price

C325S CYMAL®-5, SOL-GRADE® 1 g 76.96 [5-Cyclohexyl-1-pentyl−β−D-maltoside1] 5 g 307.84 25 g 1231.36

FW 494.5 [250692-65-0] C23H42O11 2 2 CMC (H2O) ~ 2.4-5.0 mM (0.12%) CMC (0.15M NaCl) ~ 2.0 mM 2 Aggregation Number (H2O) ~ 47 HO Purity ≥ 98% pure by HPLC analysis. HO O Percent alpha < 10 (HPLC) HO HO OH Percent cyclohexylpentanol < 0.05 (HPLC) O O pH (1% solution) 4-9 HO O Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 PRODUCT INFOMATION 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See C325 for references.

C326 CYMAL®-6, ANAGRADE® 1 g 122.72 [6-Cyclohexyl-1-hexyl−β−D-maltoside1] 5 g 490.88

NEW PRODUCTS 25 g 1963.52

FW 508.5 [228579-27-9] C24H44O11 2 CMC (H2O) ~ 0.56 mM (0.028%) 2 HO Aggregation Number (H2O) ~ 91 O Purity 99% by HPLC analysis. HO ≥ HO HO Percent alpha < 4 (HPLC) OH O O Percent cyclohexylhexanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 OH Solubility in water at 20°C ≥ 20%

PRODUCT LISTING Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract. 3. Nukaga, M., Abe, T., Venkatesan, A. M., Mansour, T. S., Bonomo, R. A., and Knox, J. R., Biochemistry 42, 13152-13159 (2003). 4. R. J. Guan, Y. Xiang, M. Wang, et al, Acta Crystallogr. D Biol. Crystallogr. 57, 1313-1315 (2001). 5. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997). INDEX

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Catalog No. Product Name/Specifications Size Price

C326LA CYMAL®-6, ANAGRADE® 1 g 174.72 [6-Cyclohexyl-1-hexyl−β−D-maltoside1] 5 g 698.88 (Contains almost no alpha isomer) 25 g 2795.52

FW 508.5 [228579-27-9] C24H44O11 2 CMC (H2O) ~ 0.56 mM (0.028%) HO PRODUCT INFORMATION Aggregation Number (H O)2 ~ 91 2 HO O Purity ≥ 99% by HPLC analysis. HO HO OH O Percent alpha < 0.5 (HPLC) O HO O Percent cyclohexylhexanol < 0.005 (HPLC) OH pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02

280 nm < 0.04 NEW PRODUCTS 260 nm < 0.06 225 nm < 0.1 See C326 for references.

C326S CYMAL®-6, SOL-GRADE® 1 g 98.80 [6-Cyclohexyl-1-hexyl−β−D-maltoside1] 5 g 395.20 25 g 1580.80

FW 508.5 [228579-27-9] C24H44O11 2 CMC (H2O) ~ 0.56 mM (0.028%) 2 HO Aggregation Number (H2O) ~ 91 HO O Purity ≥ 98% pure by HPLC analysis. HO HO OH Percent alpha < 10 (HPLC) O O Percent cyclohexylhexanol < 0.05 (HPLC) HO O pH (1% solution) 4-9 OH Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See C326 for references.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

C327 CYMAL®-7, ANAGRADE® 1 g 140.40 [7-Cyclohexyl-1-heptyl−β−D-maltoside1] 5 g 561.60 25 g 2246.40 FW 522.5 [349477-49-2] C H O 25 46 11 HO CMC (H O) ~ 0.19 mM2 (0.0099%) 2 O 2 HO Aggregation Number (H2O) ~ 150 HO HO OH Purity ≥ 99% by HPLC analysis. O O HO O Percent alpha < 4 (HPLC) OH Percent cyclohexylheptanol < 0.005 (HPLC) pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. US Patent 5,674,987 and US Patent 5,763,586. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract. 3. G. J. Babcock, M. Farzan, and J. Sodroski, J. Biol. Chem. 278, 3378-3385 (2003).

NEW PRODUCTS C327S CYMAL®-7, SOL-GRADE® 1 g 112.32 [7-Cyclohexyl-1-heptyl−β−D-maltoside1] 5 g 449.28 25 g 1797.12

FW 522.5 [349477-49-2] C25H46O11 2 CMC (H2O) ~ 0.19 mM (0.0099%) HO 2 Aggregation Number (H2O) ~ 150 HO O HO HO Purity ≥ 98% pure by HPLC analysis. OH O O Percent alpha < 10 (HPLC) HO O Percent cyclohexylheptanol < 0.05 (HPLC) OH PRODUCT LISTING pH (1% solution) 4-9 Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See C327 for references.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

C610 CYPFOS™-3, ANAGRADE® 1 g 135.51 [3-Cyclopentyl-1-propylphosphocholine] 5 g 542.06 25 g 2168.24

FW 293.3 [869548-41-4] C13H28NO4P CMC (H O) ~ 180 mM1 (5.3%) 2 O PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.

®

D365 n-DECYL-N,N-DIMETHYLAMINE-N-OXIDE, ANAGRADE 1 g 38.23 NEW PRODUCTS 5 g 152.92

FW 201.36 [2605-79-0] C12H27NO 25 g 611.69 1 CMC (H2O) ~ 10.48 mM (0.211%)

Purity ≥ 99% by HPLC analysis Purified to contain < 500 mm of equivalent peroxide. O pH (1% solution) 4-9 N Solubility in water at 20°C > 30% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06 260 nm < 0.08 1. Anatrace measurement.

D352 n-DECYL-N,N-DIMETHYLGLYCINE, ANAGRADE® 1 g 41.54 5 g 166.15

FW 243.4 [2644-45-3] C14H29O2N 25 g 664.60 1 CMC (H2O) ~ 19 mM (0.46%) O Purity ≥ 99% by HPLC analysis. N pH (1% solution) 5-8 O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 80 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

D321 n-DECYL−β−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 56.18 [n-Decyl−β−D-glucoside] 5 g 224.72 25 g 898.88

FW 320.4 [58846-77-8] C16H32O6 1 2 CMC (H2O) ~ 2.2 mM (0.070%) CMC (0.01M PO4 Buffer) ~ 2.3 mM Purity ≥ 99% by HPLC analysis. For molar volume check reference 3. HO Percent alpha < 2 (HPLC) HO O Percent decanol < 0.005 (HPLC) HO O pH (0.03% solution) 5-8 OH Solubility in water at 20°C ≥ 0.1% Conductance (0.1% solution) < 20 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Helenius, A., McCaslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 734-749 (1979). 2. Brito, R. M. M. and Vaz, W. L. C., Anal. Biochem. 152, 250-255 (1986). 3. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).

D322 n-DECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 54.20 NEW PRODUCTS [n-Decyl−β−D-maltoside] 5 g 203.25 25 g 762.19

FW 482.6 [82494-09-5] C22H42O11 1 2 CMC (H2O) ~ 1.8 mM (0.087%) CMC (0.15M NaCl) ~ 1.8 mM 2 Aggregation Number (H2O) ~ 69 Purity ≥ 99% by HPLC analysis. HO For molar volume check reference 3. HO O Percent alpha < 2 (HPLC) HO HO OH Percent decanol < 0.005 (HPLC) O O PRODUCT LISTING HO O pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Alpes, H., Apell, H.-J., Knoll, G., Plattner, H. and Riek, R., Biochim. Biophys. Acta 946, 379-388 (1988). 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970). INDEX

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Catalog No. Product Name/Specifications Size Price

D322HA n-DECYL−α−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 118.72 [n-Decyl−α−D-maltoside] 5 g 474.88 (Alpha isomer) 25 g 1899.52

FW 482.6 [168037-12-5] C22H42O11 HO HO O Purity ≥ 99% by HPLC analysis. PRODUCT INFORMATION HO Percent alpha > 94 (HPLC) HO OH O Percent < 0.005 (HPLC) O HO pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% O Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.10 260 nm < 0.15 225 nm < 0.25 NEW PRODUCTS

D322LA n-DECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 80.53 [n-Decyl−β−D-maltoside] 5 g 322.10 (Low alpha) 25 g 1288.41

FW 482.6 [82494-09-5] C22H42O11 1 2 CMC (H2O) ~ 1.8 mM (0.087%) CMC (0.15M NaCl) ~ 1.8 mM 2 Aggregation Number (H2O) ~ 69 HO Purity ≥ 99% by HPLC analysis. O For Molar volume check reference 3. HO Percent alpha < 0.2 (HPLC) HO HO OH Percent decanol < 0.005 (HPLC) O O pH (1% solution) 5-8 HO O OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 See D322 for references.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

D322S n-DECYL−β−D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 45.09 [n-Decyl−β−D-maltoside] 5 g 169.10 25 g 634.14

FW 482.6 [82494-09-5] C22H42O11 1 2 CMC (H2O) ~ 1.8 mM (0.087%) CMC (0.15M NaCl) ~ 1.8 mM 2 Aggregation Number (H2O) ~ 69 HO Purity ≥ 97% pure by HPLC analysis. HO O Percent alpha < 5 (HPLC) HO HO Percent decanol < 0.05 (HPLC) OH O O pH (1% solution) 4-9 HO O Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 100 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 PRODUCT INFOMATION 260 nm < 0.1 225 nm < 0.2 See D322 for references.

D323 n-DECYL−β−D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Decyl−β−D-thioglucoside] 5 g 307.99

NEW PRODUCTS 25 g 1154.94

FW 336.4 [98854-16-1] C16H32O5S CMC (methanol/water) ~ 0.9 mM1 (0.30%) HO Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 2 (HPLC) HO S pH (1% solution2) 5-8 OH Solubility in methanol:water2 at 20°C ≥ 1% Conductance (1% solution2) < 40 μS Percent fluorescence due to a 0.1% detergent solution2 at 345 nm < 10 2

PRODUCT LISTING Absorbance of a 1% detergent solution : 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 NOTE: n-Decyl−β−D-thioglucopyranoside is insoluble in water. 1. Anatrace measurement. 2. Solvent: (1:1) v/v methanol : water.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

D335 n-DECYL−β−D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Decyl−β−D-thiomaltoside] 5 g 307.99 25 g 1154.94

FW 498.6 [14565-56-4] C22H42O10S CMC (H O) ~ 0.9 mM1 (0.045%) 2 HO 2 Aggregation Number (H2O) ~ 75 PRODUCT INFORMATION HO O Purity ≥ 99% by HPLC analysis. HO HO OH Percent alpha < 2 (HPLC) O O pH (1% solution) 5-8 HO S Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2

1. Anatrace measurement. NEW PRODUCTS 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract.

D354 DECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12

FW 236.0 [10108-87-9] C13H30ClN 100 g 259.58 1 CMC (H2O) ~ 2.95 mM (0.07%) Purity ≥ 95% pure by titration. pH (1% solution) 5.5-8.5 N Solubility in water at 20°C ≥ 30% Cl Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement. 2. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). 3. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999).

D380 DEOXYCHOLIC ACID, SODIUM SALT, ANAGRADE® 5 g 49.00 [3α,12α-Dihydroxy-5β-cholan-24-oic acid, monosodium salt] 25 g 196.00 100 g 627.20

FW 414.6 [302-95-4] C24H39O4Na 1 CMC (H2O) ~ 6 mM (0.24%) 2 O Aggregation Number (H2O) ~ 22 OH Purity ≥ 98% pure by HPLC analysis. ONa pH (1% solution) 5-9 Solubility in water at 20° C ≥ 5% Absorbance of a 1% detergent solution 340 nm < 0.02 HO 280 nm < 0.04 260 nm < 0.06 INDEX

1. Anatrace measurement. 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html

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Catalog No. Product Name/Specifications Size Price

D412 DIFOS™-12, ANAGRADE® 250 mg Please Inquire [Dodecyl-1,2-diphosphocholine] 1 g 5 g

FW 532.6 [869584-01-0] C22H50N2O8P2 N 1 CMC (H2O) 1.18 mM (0.063%) O Purity ≥ 99% by HPLC analysis. O P O pH (1% solution) 5-8 O O Solubility in water at 0-5oC ≥ 20% O PO N O Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.2

PRODUCT INFOMATION 1. Anatrace measurement.

DH325 2,6-DIMETHYL-4-HEPTYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 76.96 5 g 307.84 25 g 1231.36

FW 468.5 [869638-31-3] C21H40O11 1 CMC (H2O) ~ 27.5 mM (1.2%) HO

Purity ≥ 99% by HPLC analysis. HO O NEW PRODUCTS Percent alpha < 15% HO HO OH Percent (2-6-dimethyl-4-heptanol) < 0.005% O O pH (1% solution) 5-8 HO O OH Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06

PRODUCT LISTING 225 nm < 0.1 1. Anatrace measurement.

D330 DIMETHYLDECYLPHOSPHINE OXIDE, ANAGRADE® 1 g 39.00 [Decyldimethyl-phosphine oxide] 5 g 156.00 25 g 624.00

FW 218.3 [2190-95-6] C12H27OP 1 CMC (H2O) ~ 4.66 mM (0.10%) 1 Aggregation Number (H2O) ~ 31 Purity ≥ 99% pure by HPLC analysis. pH (1% solution) 5-8 P Solubility in water at 0-5oC ≥ 20% O Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 INDEX 260 nm < 0.1 1. Herrmann, K. W., Brushmiller, J. G. and Courchene, W. L., Micellar Properties of Dimethlalkylphosphine Oxide Solutions 70, Number 9, 2909 (1966).

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Catalog No. Product Name/Specifications Size Price

D328 DIMETHYLOCTYLPHOSPHINE OXIDE, ANAGRADE® 1 g 39.00 [Octyl dimethyl-phosphine oxide] 5 g 156.00 25 g 624.00

FW 190.1 [13232-68-3] C10H23OP 1 CMC (H2O) ~ 40 mM (0.76%) 1 Aggregation Number (H2O) ~ 41 PRODUCT INFORMATION Purity 99% pure by HPLC analysis. P ≥ O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Herrmann, K. W., Brushmiller, J. G. and Courchene, W. L., Micellar Properties of Dimethlalkylphosphine Oxide Solutions 70, Number 9, 2909 (1966). NEW PRODUCTS D360 n-DODECYL-N,N-DIMETHYLAMINE-N-OXIDE, ANAGRADE® 1 g 30.97 [Lauryldimethylamine-N-oxide / LDAO / DDAO / 5 g 123.86 N,N-Dimethyl-1-dodecanamine-N-oxide] 25 g 495.47

FW 229.41 [1643-20-5] C14H31NO 5 5 CMC (H2O) ~ 1-2 mM (0.023%) CMC (0.1M NaCl) ~ 0.14 mM 6 Aggregation Number (H2O) ~ 76 O Purity ≥ 99% by HPLC analysis. N Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 6-8.5 Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06 260 nm < 0.08 1. Michel, H., J. Mol. Biol. 158, 567-572 (1982). 2. Malkin, R., Arch. Biochem. Biophys. 169, 77-83 (1975). 3. Reithmeier, A. F., et al, Biochem. 32, 1172-1179 (1993). 4. Dawkins, D. J., et al, in Crystallization of Membrane Proteins (Hartmut Michel, Ed.) 125-137, CRC Press, Boca Raton (1991). 5. Herrmann, K. W., J. Phys. Chem. 66, 292 (1962). 6. Herrmann, K. W. J., Colloid Interface Sci. 22, 352 (1966). INDEX

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Catalog No. Product Name/Specifications Size Price

D360S n-DODECYL-N,N-DIMETHYLAMINE-N-OXIDE, SOL-GRADE® 1 g 24.77 [Lauryldimethylamine-N-oxide / LDAO / DDAO / 5 g 99.11 N,N-Dimethyl-1-dodecanamine-N-oxide] 25 g 396.43

FW 229.41 [1643-20-5] C14H31NO 5 5 CMC (H2O) ~ 1-2 mM (0.023%) CMC (0.1M NaCl) ~ 0.14 mM Purity ≥ 95% pure by HPLC analysis. O Purified to contain < 500 μM of equivalent peroxide. N pH (1% solution) 5-9 Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.1 260 nm < 0.2 PRODUCT INFOMATION Note: This material contains lower organic peroxide levels than the commercial grade, but higher levels than our ANAGRADE® detergent. The amount of equivalent peroxide is listed on the Certificate of Analysis for each lot. See D360 for references.

D350 n-DODECYL-N,N-DIMETHYLGLYCINE, ANAGRADE® 1 g 41.54 (Major component of the industrial detergent, Empigen BB®) 5 g 166.15 25 g 664.60 NEW PRODUCTS FW 271.4 [683-10-3] C16H33O2N 1 CMC (H2O) ~ 1.5 mM (0.041%)

Purity ≥ 99% by HPLC analysis. O pH (1% solution) 5-8 N O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 80 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 PRODUCT LISTING 1. Personal communication from Professor R. M. Garavito (Michigan State University).

D350S n-DODECYL-N,N-DIMETHYLGLYCINE, SOL-GRADE® 1 g 31.35 (Major component of the industrial detergent, Empigen BB®) 5 g 125.38 25 g 501.52

FW 271.4 [683-10-3] C16H33O2N 1 CMC (H2O) ~ 1.5 mM (0.041%) Purity ≥ 98% pure by HPLC analysis. O pH (1% solution) 4-9 N O Solubility in water at 20oC ≥ 30% Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.2 280 nm < 0.4 260 nm < 0.8 1. Personal communication from Professor R. M. Garavito (Michigan State University). INDEX

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Catalog No. Product Name/Specifications Size Price

D318 n-DODECYL−β−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.30 [n-Dodecyl−β−D-glucoside] 5 g 222.60 25 g 834.74

FW 348.5 [59122-55-3] C18H36O6 1 CMC (H2O) ~ 0.19 mM (0.0066%) PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. HO For molar volume check reference 2. HO O Percent alpha < 2 (HPLC) HO O Percent dodecanol < 0.005 (HPLC) OH pH (0.005% solution) 5-8 Solubility in water at 20°C ≥ 0.008% Conductance (0.005% solution) < 10 μS Percent fluorescence due to a 0.005% detergent solution at 345 nm < 10 Absorbance of a 0.005% detergent solution: 340 nm < 0.06 280 nm < 0.06

260 nm < 0.06 NEW PRODUCTS 225 nm < 0.2 1. Helenius, A., McCaslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 734-749 (1979). 2. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).

D345 n-DODECYL−β−IMINODIPROPIONIC ACID, DISODIUM SALT 5 g 25.79 (Disodium-N-lauryl−β−iminodipropionate) 25 g 103.17 100 g 330.14

FW 373.4 [3655-00-3] C18H33NO4Na2 O ONa

Purity ≥ 97% pure by HPLC analysis. pH (0.5% solution) 9-12 N O Solubility in water at 0-5°C ≥ 20% Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 NaO Absorbance of a 0.5% detergent solution 340 nm < 0.05 280 nm < 0.1 260 nm < 0.3 NOTE: Heating may be required to dissolve detergent.

D346 n-DODECYL−β−IMINODIPROPIONIC ACID, MONOSODIUM SALT 5 g 25.79 (Monosodium-N-lauryl−β−iminodipropionate) 25 g 103.17 100 g 330.14 FW 351.5 [14960-06-6] C H NO Na O 18 34 4 OH Purity ≥ 97% pure by HPLC analysis. pH (0.5% solution) 6.0-8.5 Solubility in water at 0-5°C N ≥ 20% O Off white in color NaO Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10

Absorbance of a 0.5% detergent solution 340 nm < 0.05 INDEX 280 nm < 0.1 260 nm < 0.3 NOTE: Heating may be required to dissolve detergent.

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Catalog No. Product Name/Specifications Size Price

D310HA n-DODECYL−α−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 118.72 [n-Dodecyl−α−D-maltoside / Lauryl maltoside] 5 g 474.88 (Alpha isomer) 25 g 1899.52

FW 510.6 [116183-64-3] C24H46O11 1-2 CMC (H2O) ~ 0.15 mM (0.0076%) 2 Aggregation Number (H2O) ~ 90 HO

Purity ≥ 99% by HPLC analysis. HO O Percent alpha > 94 (HPLC) HO HO OH O Percent dodecanol < 0.005 (HPLC) O HO pH (1% solution) 5-8 OH O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10

PRODUCT INFOMATION Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.10 260 nm < 0.15 225 nm < 0.25 1. Anatrace measurement. 2. VanAken, T., Foxall-VanAken, S., Castleman, S. and Ferguson-Miller, S., Methods Enzymol. 125, 27-35 (1986).

D310 n-DODECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 63.00 NEW PRODUCTS [n-Dodecyl−β−D-maltoside / Lauryl maltoside / 5 g 236.25 Dodecyl 4-O−α−D-glucopyranosyl−β−D-glucopyranoside] 25 g 885.94

FW 510.6 [69227-93-6] C24H46O11 1 2 CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM 1-2 Aggregation Number (H2O) ~ 78-149 Purity ≥ 99% by HPLC analysis. HO For molar volume check reference 3. HO O Percent alpha < 2 (HPLC) HO HO PRODUCT LISTING OH Percent dodecanol < 0.005 (HPLC) O O pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. VanAken, T., Foxall-VanAken, S., Castleman, S. and Ferguson-Miller, S., Methods Enzymol. 125, 27-35 (1986). 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970). INDEX

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Catalog No. Product Name/Specifications Size Price

D310A n-DODECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 56.70 [n-Dodecyl−β−D-maltoside / Lauryl maltoside / 5 g 212.63 Dodecyl 4-O−α−D-glucopyranosyl−β−D-glucopyranoside] 25 g 797.34 (Contains up to 15% alpha isomer)

FW 510.6 [69227-93-6] C24H46O11 1 2 PRODUCT INFORMATION CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM Aggregation Number (H O)1-2 ~ 78-149 2 HO

Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 15 (HPLC) HO HO OH Percent dodecanol < 0.005 (HPLC) O O pH (1% solution) 5-8 HO O OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 NEW PRODUCTS 260 nm < 0.06 225 nm < 0.1 See D310 for references.

D310LA n-DODECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 87.00 [n-Dodecyl−β−D-maltoside / Lauryl maltoside / 5 g 326.25 Dodecyl 4-O−α−D-glucopyranosyl−β−D-glucopyranoside] 25 g 1223.44 (Low alpha)

FW 510.6 [69227-93-6] C24H46O11 1 2 CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM 2 Aggregation Number (H2O) ~ 78-149 Purity ≥ 99% by HPLC analysis. Percent alpha < 0.2 (HPLC) Percent dodecanol < 0.005 (HPLC) HO pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% HO HO OH Conductance (10% solution) < 40 μS O O Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 HO O OH Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 See D310 for references. INDEX

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Catalog No. Product Name/Specifications Size Price

D310S n-DODECYL−β−D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 50.50 [n-Dodecyl−β−D-maltoside / Lauryl maltoside / 5 g 189.00 Dodecyl 4-O−α−D-glucopyranosyl−β−D-glucopyranoside] 25 g 708.75

FW 510.6 [69227-93-6] C24H46O11 1 2 CMC (H2O) ~ 0.17 mM (0.0087%) CMC (0.2 M NaCl) ~ 0.12 mM 2 Aggregation Number (H2O) ~ 78-149

Purity ≥ 98% pure by HPLC analysis. HO Percent alpha < 5 (HPLC) O Percent dodecanol < 0.05 (HPLC) HO HO HO pH (1% solution) 4-9 OH O O Solubility in water at 20°C ≥ 20% HO O Conductance (10% solution) < 100 μS OH Absorbance of a 1% detergent solution: 340 nm < 0.05 PRODUCT INFOMATION 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See D310 for references.

D310T n-DODECYL-d25−β−D-MALTOPYRANOSIDE 100 mg 407.04

(n-Dodecyl-d25−β−D-maltoside, Lauryl maltoside) 250 mg 915.84

NEW PRODUCTS 500 mg 1740.10

FW 535.8 [849110-74-3] C24D25H21O11 1 CMC (H2O) ~ 0.2 mM HO Purity ≥ 97% pure by HPLC analysis. HO O Percent alpha < 15 (HPLC) HO HO Percent dodecanol < 0.05 (HPLC) OH O D D D D D D D D D O D D D pH (1% solution) 5-8 HO O Solubility in water at 20°C 10% OH D ≥ D D D D D D D D D D D D Conductance (1% solution) < 200 μS

PRODUCT LISTING Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.25 260 nm < 0.25 225 nm < 0.8 1. CMC value for the undeuterated compound.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

D342 n-DODECYL−β−D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 (n-Dodecyl−β−D-thiomaltoside) 5 g 307.99 25 g 1154.94

FW 526.6 [148565-58-6] C24H46O10S 1 CMC (H2O) ~ 0.05 mM (0.0026%) 2 HO Aggregation Number (H2O) ~ 126 PRODUCT INFORMATION HO O Purity ≥ 98% pure by HPLC analysis. HO HO Percent alpha < 2 (HPLC) OH O O pH (1% solution) 5-8 HO S Solubility in water at 20°C ≥ 10% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental NEW PRODUCTS services contract. 3. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997).

D355 DODECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12

FW 263.89 [112-00-5] C15H34ClN 100 g 259.58 1 CMC (H2O) ~ 0.046 mM (0.0012%) 4,5 Aggregation Number (H2O) ~ 44-61 Purity ≥ 95% pure by titration. N pH (1% solution) 5.5-8.5 Cl Solubility in water at 20°C ≥ 30% Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 Note: Measured as the bromide salt. 1. Anatrace measurement. 2. J. Mata, J. Patel, N. Jain, et al, J. Colloid Interface Sci. Dec. 22, (2005). 3. H. Matsubara, T. Nakano, T. Matsuda, et al, Langmuir 21, 8131-8137 (2005). 4. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). 5. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999).

D2001 DTE 1 g 11.00 [Dithioerythritol] 5 g 46.00 25 g 185.00

FW 154.25 [6892-68-8] C4H10O2S2 Form: White crystalline powder. OH Assay (I ): ≥ 99% SH 2 HS Chromatography (TLC [(CH2Cl2:Et2O/2:1)]): Homogeneous OH Melting Point: ≥ 81°C INDEX

Absorance: (283 nm, 0.02 M, H2O): ≥ 0.04

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Catalog No. Product Name/Specifications Size Price

D1000 DTNB 5 g 43.00 [5,5'-Dithiobis-(2-nitrobenzoic) acid] 25 g 177.00

FW 396.36 [69-78-3] C14H6N2O8S2 O Form: Light yellow crystalline powder N+ O- Assay (Titration with 0.1N NaOH in ): ≥ 99.0% S OH Melting Range: 236-248° C S -O O N+ O OOH

D2000 DTT, CLELAND'S REAGENT 5 g 19.76 [1,4-Dithiothreitol] 25 g 79.04

PRODUCT INFOMATION 100 g 269.36

FW 154.2 [27565-41-9] C4H10O2S2

Purity (SH) Iodometric titration > 97% OH o Solubility in water at 20 C: 3% SH Melting Point: 41-43oC HS Oxidized dithiothreitol < 0.5% OH Appearance: White Powder NEW PRODUCTS D2000A DTT, CLELAND'S REAGENT, ANAGRADE® 1 g 16.64 [1,4-Dithiothreitol] 5 g 66.56 25 g 266.24

FW 154.24 [27565-41-9] C4H10O2S2 Purity (SH) Iodometric titration ≥ 99.5% Absorbance: (280 nm, 0.1M, 1cm): < 0.08 Absorbance: (260 nm, 0.1M, 1cm): < 0.40 OH o Melting Point: 40-43 C SH HS PRODUCT LISTING Oxidized DTT: < 0.3% OH DNase: None detected RNase: None detected Protease: None detected Storage: -20oC Appearance: White Crystalline Powder

NOTES: INDEX

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Catalog No. Product Name/Specifications Size Price

E1000 EDTA 100 g 17.00 [Ethylene diaminetetracetic acid, Disodium salt, Dihydrate] 500 g 46.00

. FW 372.24 [6381-92-6] C10H14N2Na2O8 2H2O Form: White crystalline powder PRODUCT INFORMATION Assay (Titration): 99.0 to 101.0% O O Absorbance: (280 nm, 0.1M, 1cm): < 0.05 OH Absorbance: (260 nm, 0.1M, 1cm): < 0.2 NaO N Insoluble matter: < 0.005% 2H2O N ONa pH (5%, H2O): 4.0 to 6.0 HO Nitrilotriacetic Acid: < 0.1% O O Heavy Metals (Pb): < 0.005% Iron (Fe): < 0.01% Arsenic (As): < 1 ppm Calcium (Ca): Passes test

Solubility (0.1M, H2O): Complete clear and colorless NEW PRODUCTS DNase (endo): None detected DNase (exo): None detected RNase: None detected

E1001 EGTA 10 g 66.00 [-O,O'-bis(2-aminoethyl)-N,N,N',N'-tetracetic acid] 50 g 303.00

FW 380.35 [67-42-5] C14H24N2O10 Assay (Titration): ≥ 99.0% OH Absorbance (280 nm, 0.1M in 1N NaOH, 1cm): < 0.10 Loss on Drying: < 0.5% O O N Cadmium (Cd): < 5 ppm O OH Chloride (Cl): < 0.005% O O Cobalt (Co): < 5 ppm N Copper (Cu): < 5 ppm HO OH Iron (Fe): < 5 ppm O Lead (Pb): < 5 ppm Nickel (Ni): < 5 ppm

Sulfate (SO4): < 0.02% Zinc (Zn): < 5 ppm

E2000 NEM 5 g 36.00 [N-Ethylmaleimide] 10 g 59.00 25 g 121.00

FW 125.13 [128-53-0] C6H7NO2 Form: White crystalline powder O N O Melting Point: 44-45°C Storage: +4°C INDEX

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Catalog No. Product Name/Specifications Size Price

F300 FOS-CHOLINE®-8, ANAGRADE® 1 g 141.78 [n-Octylphosphocholine] 5 g 567.12 25 g 2270.11

FW 295.4 [53255-89-3] C13H30NO4P CMC (H O) ~ 114 mM1 (3.4%) 2 O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION 2. O. Vinogradova, F. Sonnichsen, C. R. Sanders, J. Biomol. NMR 11, 381-386 (1998).

F300S FOS-CHOLINE®-8, SOL-GRADE® 1 g 113.22 [n-Octylphosphocholine] 5 g 452.88 25 g 1811.52

FW 295.4 [53255-89-3] C13H30NO4P 1 CMC (H2O) ~ 114 mM (3.4%) O

NEW PRODUCTS Purity 97% by HPLC analysis. ≥ O PO pH (1% solution) 4-9 N O 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 See F300 for references.

F302 FOS-CHOLINE®-9, ANAGRADE® 1 g 141.78 PRODUCT LISTING [n-Nonylphosphocholine] 5 g 567.12 25 g 2270.11

FW 309.4 [253678-64-7] C14H32NO4P 1 CMC (H2O) ~ 39.5 mM (1.2%) O Purity 99% by HPLC analysis. ≥ O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

F302S FOS-CHOLINE®-9, SOL-GRADE® 1 g 113.22 [n-Nonylphosphocholine] 5 g 452.88 25 g 1811.52

FW 309.4 [253678-64-7] C14H32NO4P 1 CMC (H2O) ~ 39.5 mM (1.2%) O

Purity ≥ 97% by HPLC analysis. O PO PRODUCT INFORMATION pH (1% solution) 4-9 N O 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. Anatrace measurement.

F304 FOS-CHOLINE®-10, ANAGRADE® 1 g 126.48 NEW PRODUCTS [n-Decylphosphocholine] 5 g 505.92 25 g 2023.68

FW 323.4 [70504-28-8] C15H34NO4P 1 CMC (H2O) ~ 11 mM (0.35%) O Purity 99% by HPLC analysis. ≥ O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Cortes, D. M. and Perozo, E., Biochem. 36, 10343-10352 (1997).

F304S FOS-CHOLINE®-10, SOL-GRADE® 1 g 100.98 [n-Decylphosphocholine] 5 g 403.92 25 g 1615.68

FW 323.4 [70504-28-8] C15H34NO4P O 1 CMC (H2O) ~ 11 mM (0.35%) O PO O Purity ≥ 97% pure by HPLC analysis. N pH (1% solution) 4-9 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 See F304 for references. INDEX

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Catalog No. Product Name/Specifications Size Price F306 FOS-CHOLINE®-11, ANAGRADE® 1 g 141.68 [n-Undecylphosphocholine] 5 g 567.12

FW 337.4 [253678-65-8] C16H36NO4P 25 g 2270.11 1 CMC (H2O) ~ 1.85 mM (0.062%) 2 Aggregation Number (H2O) ~ 18 O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 ∝S Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental PRODUCT INFOMATION services contract.

F306S FOS-CHOLINE®-11, SOL-GRADE® 1 g 113.22 [n-Undecylphosphocholine] 5 g 452.88

FW 337.4 [253678-65-8] C16H36NO4P 25 g 1811.52 1 CMC (H2O) ~ 1.85 mM (0.062%) 2 O Aggregation Number (H2O) ~ 18 O PO NEW PRODUCTS Purity ≥ 97% pure by HPLC analysis. N O pH (1% solution) 4-9 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 See F306 for refrences.

F308 FOS-CHOLINE®-12, ANAGRADE® 1 g 126.48 PRODUCT LISTING [n-Dodecylphosphocholine] 5 g 505.92

FW 351.5 [29557-51-5] C17H38NO4P 25 g 2023.68 1 CMC (H2O) ~ 1.5 mM (0.047%) 6 Aggregation Number (H2O) ~ 54 Purity ≥ 99% by HPLC analysis. O O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. C. Fares, D. S. Libich, G. Harauz, FEBS J. 273, 601-614 (2006). 3. R. Brunecky, S. Lee, P. W. Rzepecki, et al, Biochemistry 44, 16064-16071 (2005). 4. K. Oxenoid, J. J. Chou, Proc. Natl. Acad. Sci. USA 102, 10870-10875 (2005).

INDEX 5. Uteng, M., Hauge, H. H., Markwick, P. R. L., Fimland, G., Mantzilas, D., Nissen-Meyer, J., and Muhle-Goll, C., Biochem. 42, 11417-11426 (2003). 6. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract.

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Catalog No. Product Name/Specifications Size Price

F308D FOS-CHOLINE®-12, DEUTERATED 100 mg 202.98

[n-Dodecylphosphocholine (D38)] 500 mg 774.18 1 g 1417.80

FW 389.79 [130890-78-7] C17D38NO4P CMC (H O) ~ 1.5 mM (0.047%)1 2 O D D D D D D D D D D D D D D D 3C O PO PRODUCT INFORMATION N Purity ≥ 90% pure by HPLC analysis. O D D 3 C D D D D D D D D D D D D pH (1% solution) 4-9 D 3C D D 1. CMC value for the undeuterated compound. 2. See F308 for references.

F308S FOS-CHOLINE®-12, SOL-GRADE® 1 g 100.98 [n-Dodecylphosphocholine] 5 g 403.92 25 g 1615.68

FW 351.5 [29557-51-5] C17H38NO4P 1 O CMC (H2O) ~ 1.5 mM (0.047%)

O PO NEW PRODUCTS Purity ≥ 97% pure by HPLC analysis. N O pH (1% solution) 4-9 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 1. See F308 for references.

F310 FOS-CHOLINE®-13, ANAGRADE® 1 g 141.78 [n-Tridecylphosphocholine] 5 g 567.12 25 g 2270.11

FW 365.5 [85775-42-4] C18H40NO4P 1 CMC (H2O) ~ 0.75 mM (0.027%) 2 Aggregation Number (H2O) ~ 87 O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract. INDEX You can search for a detergent by catalog number (pgs. 121-122), chemical name (pgs. 123-127), or CAS registry number (pgs. 128-131). A search program is also available at www.anatrace.com.

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Catalog No. Product Name/Specifications Size Price

F310S FOS-CHOLINE®-13, SOL-GRADE® 1 g 113.22 [n-Tridecylphosphocholine] 5 g 452.88 25 g 1811.52 FW 365.5 [85775-42-4] C H NO P 18 40 4 O CMC (H O) ~ 0.75 mM1 (0.027%) 2 O PO N O Purity ≥ 97% by HPLC analysis. pH (1% solution) 4-9 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 See F310 for reference. PRODUCT INFOMATION

F312 FOS-CHOLINE®-14, ANAGRADE® 1 g 141.78 [n-Tetradecylphosphocholine] 5 g 567.12 25 g 2270.11

FW 379.5 [77733-28-9] C19H42NO4P 1 CMC (H2O) ~ 0.12 mM (0.0046%) 2 Aggregation Number (H2O) ~ 108 O NEW PRODUCTS Purity 99% by HPLC analysis. ≥ O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental PRODUCT LISTING services contract.

F312D FOS-CHOLINE®-14, DEUTERATED 100 mg 253.98

[n-Tetradecylphosphocholine-d42] 500 mg 1015.92 1 g 1828.66

FW 421.5 [869638-98-2] C19D42NO4P CMC (H O) ~ 0.12 mM (0.0051%)1 2 D D O D D D D D D D D D D D D D D C D Purity ≥ 90% pure by HPLC analysis. 3 O PO N D D3C O pH (1% solution) 4-9 D3C D D D D D D D D D D D D D D D D Solubility in water at 20°C > 1% 1. CMC value for the undeuterated compound. 2. See F312 for references. INDEX

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Catalog No. Product Name/Specifications Size Price

F312S FOS-CHOLINE®-14, SOL-GRADE® 1 g 113.22 [n-Tetradecylphosphocholine] 5 g 452.88 25 g 1811.52

FW 379.5 [77733-28-9] C19H42NO4P 1 CMC (H2O) ~ 0.12 mM (0.0046%) O 2 O PO Aggregation Number (H2O) ~ 108 PRODUCT INFORMATION N O Purity ≥ 97% pure by HPLC analysis. pH (1% solution) 4-9 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.2 See F312 for references.

® ®

F314 FOS-CHOLINE -15, ANAGRADE 1 g 141.78 NEW PRODUCTS [n-Pentadecylphosphocholine] 5 g 567.12 25 g 2270.11

FW 393.5 [146801-07-2] C20H44NO4P CMC (H O) ~ 0.07 mM1 (0.0027%) 2 O Aggregation Number (H O)2 ~ 131 2 O PO Purity 99% by HPLC analysis. ≥ N O pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) <200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract.

F314S FOS-CHOLINE®-15, SOL-GRADE® 1 g 113.22 [n-Pentadecylphosphocholine] 5 g 452.88 25 g 1811.52

FW 393.5 [146801-07-2] C20H44NO4P 1 CMC (H2O) ~ 0.07 mM (0.0027%) 2 O Aggregation Number (H2O) ~ 131 O PO Purity ≥ 97% by HPLC analysis. N O pH (1% solution) 4-9 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) <500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 INDEX 260 nm < 0.2 See F314 for references.

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Catalog No. Product Name/Specifications Size Price

F316 FOS-CHOLINE®-16, ANAGRADE® 1 g 141.78 [n-Hexadecylphosphocholine / 2-[[(Hexadecyloxyl)hydroxyphosphinyl]oxy]- 5 g 567.12 N,N,N-trimethyl-ethanaminium, hydroxide, inner salt] 25 g 2270.11

FW 407.5 [58066-85-6] C21H46NO4P 1 CMC (H2O) ~ 0.013 mM (0.00053%) 2 Aggregation Number (H2O) ~ 178 O Purity 99% by HPLC analysis. ≥ O PO pH (1% solution) 5-8 N O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) <200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 PRODUCT INFOMATION 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract.

F316S FOS-CHOLINE®-16, SOL-GRADE® 1 g 113.22 [n-Hexadecylphosphocholine / 2-[[(Hexadecyloxyl)hydroxyphosphinyl]oxy]- 5 g 452.88 N,N,N-trimethyl-ethanaminium, hydroxide, inner salt] 25 g 1811.52

NEW PRODUCTS FW 407.5 [58066-85-6] C21H46NO4P 1 CMC (H2O) ~ 0.013 mM (0.00053%) O 2 Aggregation Number (H2O) ~ 178 O PO N O Purity ≥ 97% by HPLC analysis. pH (1% solution) 4-9 10% solution may appear slightly hazy or cloudy at room temperature. Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2

PRODUCT LISTING 260 nm < 0.2 See F316 for references.

FCI09 FOS-CHOLINE®-ISO-9, ANAGRADE® 1 g 141.78 [2,6-Dimethyl-4-heptylphosphocholine] 5 g 567.12 25 g 2268.48

FW 309.0 [869646-90-2] C14H32NO4P 1 CMC (H2O) ~ 32 mM (0.99%) O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 4-9 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 300 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.10

INDEX 1. Anatrace measurement. Note: This product is a mixture of closely related dimethylheptylphosphocholines. The major component is 2,6-Dimethyl-4-heptylphosphocholine (95-99%) and the minor component is 4,6-Dimethyl-4-heptylphosphocholine (0-5%). 64 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Product Listing SERVICE INFORMATION

Catalog No. Product Name/Specifications Size Price

FCI11 FOS-CHOLINE®-ISO-11, ANAGRADE® 1 g 132.91 [2,8-Dimethyl-5-nonylphosphocholine] 5 g 531.63 25 g 2126.55

FW 337.4 [869647-65-4] C16H36NO4P

1 PRODUCT INFORMATION CMC (H2O) ~ 26.6 mM (0.9%) O O PO Purity ≥ 99% by HPLC analysis. pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. NEW PRODUCTS FCI116 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® 1 g 143.11 [Undecyl-6-phosphocholine] 5 g 572.42 25 g 2289.70

FW 337.16 [869647-81-4] C16H36NO4P 1 CMC (H2O) ~ 25.8 mM (0.87%) O Purity ≥ 99% pure by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.2 1. Anatrace measurement.

FCU110 FOS-CHOLINE®-UNSAT-11-10, ANAGRADE® 1 g 132.91 [10-Undecylenyl-1-phosphocholine] 5 g 531.63 25 g 2126.55

FW 335.4 [121045-77-0] C16H34NO4P 1 CMC (H2O) ~ 6.2mM (0.21%) O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

F208 FOS-MEA®-8, ANAGRADE® 1 g 132.91 [Octylphospho-N-methylethanolamine] 5 g 531.63 25 g 2126.55

FW 267.01 [104702-33-2] C11H26NO4P CMC (H O) ~ 22.0 mM1 (0.59%) 2 O Purity ≥ 99% pure by HPLC analysis. O PO H pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 1% H Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION

F210 FOS-MEA®-10, ANAGRADE® 1 g 132.91 [Decylphospho-N-methylethanolamine] 5 g 531.63 25 g 2126.55

FW 295.01 [557788-85-9] C13H30NO4P 1 CMC (H2O) ~ 5.25 mM (0.15%) O Purity ≥ 99% pure by HPLC analysis. O PO NEW PRODUCTS pH (1% solution) 3-8 H O o N Solubility in water at 20 C ≥ 1% H Conductance (1% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.

® ®

PRODUCT LISTING F212 FOS-MEA -12, ANAGRADE 1 g 132.91 [Dodecylphospho-N-methylethanolamine] 5 g 531.63 25 g 2126.55

FW 323.01 [129274-39-1] C15H34NO4P CMC (H O) ~ 0.43 mM1 (0.014%) 2 O Purity ≥ 99% pure by HPLC analysis. O PO pH (0.1% solution) 3-8 H O Solubility in water at 20oC 0.01% N ≥ H Conductance (0.1% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

FF360 FOSFEN™-9, ANAGRADE® Please inquire. [Nonylphenylphosphocholine]

FW 385.21 [869648-98-6] C20H36NO4P 1 CMC (H2O) ~ 1.35 mM (0.052%) 2 O 95% pure by HPLC analysis. PRODUCT INFORMATION pH (1% solution) 4-9 O PO O Solubility in water at 0-5oC ≥ 20% N Conductance (10% solution) < 500 μS 1. Anatrace measurement. 2. Product is a mixture of alkylphenylphosphocholines. The major component is the para substituted product while the minor components are the meta and ortho substituted products.

G1000 GUANIDINE HYDROCHLORIDE 100 g 36.00 500 g 101.00 . FW 95.53 [50-01-1] (NH2)2C=NH HCl Form: White clear crystals NEW PRODUCTS Assay (elemental analysis): ≥ 99.5% Absorbance (260nm, 6M, 1cm): < 0.03 Absorbance (225nm, 6M, 1cm): < 0.2 NH Melting Point: 184 + 3°C HCl

Solubility (6M H2O): Clear and colorless Aldehyde: None detected H2NNH2

Sulfate (SO4): < 50 ppm Arsenic (As): < 1 ppm Copper (Cu): < 1 ppm Iron (Fe): < 2 ppm Lead (Pb): < 5 ppm Zinc (Zn): < 1 ppm Endonuclease: None detected Ribonuclease: None detected Protease: None detected

H108 HEGA®-8, ANAGRADE® 1 g 79.31 [Octanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96

FW 351.5 [869652-63-1] C16H33NO7 CMC (H O) ~ 109 mM1 (3.8%) OH 2 O Purity ≥ 99% by HPLC analysis. N pH (1% solution) 5-8 OH H Solubility in water at 0-5°C ≥ 20% OH H OH Conductance (10% solution) < 100 μS HO OH H Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 INDEX 1. Anatrace measurement.

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Catalog No. Product Name/Specifications Size Price

H109 HEGA®-9, ANAGRADE® 1 g 79.31 [Nonanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96

FW 365.5 [869653-90-7] C17H35NO7 1 CMC (H2O) ~ 39 mM (1.4%) OH Purity ≥ 99% by HPLC analysis. O pH (1% solution) 5-8 N Solubility in water at 0-5°C ≥ 20% OH H Conductance (10% solution) < 100 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H OH HO OH H Absorbance of a 1% detergent solution: 340 nm < 0.05 H 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. PRODUCT INFOMATION

H110 HEGA®-10, ANAGRADE® 1 g 79.31 [Decanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96

FW 379.5 [139361-84-5] C18H37NO7 1 CMC (H2O) ~ 7.0 mM (0.26%) Purity ≥ 99% by HPLC analysis. OH NEW PRODUCTS pH (1% solution) 5-8 O

Solubility in water at 0-5°C ≥ 10% N OH Conductance (10% solution) < 100 μS H Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 OH H OH HO OH Absorbance of a 1% detergent solution: 340 nm < 0.05 H 280 nm < 0.08 H 260 nm < 0.1 NOTE: Heating may be required to dissolve the detergent. 1. Anatrace measurement. 2. Cortes, D. M. and Perozo, E., Biochem. 36, 10343-10352 (1997). PRODUCT LISTING

H111 HEGA®-11, ANAGRADE® 1 g 79.31 [Undecanoyl-N-hydroxyethylglucamide] 5 g 317.24 25 g 1268.96

FW 393.5 [869654-10-4] C19H39NO7 1 CMC (H2O) ~ 1.4 mM (0.055%) OH Purity ≥ 99% by HPLC analysis. O pH (0.5% solution) 5-8 N OH Solubility in water at 0-5°C ≥ 0.5% H OH Conductance (0.5% solution) < 100 μS H OH HO OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H Absorbance of a 0.5% detergent solution: 340 nm < 0.05 H 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

H300 n-HEPTYL−β−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.43 [n-Heptyl−β−D-glucoside] 5 g 233.71 25 g 934.84

FW 278.4 [78617-12-6] C13H26O6 1 CMC (H2O) ~ 70 mM (1.9%)

Purity ≥ 99% by HPLC analysis. PRODUCT INFORMATION Percent alpha < 2 (HPLC) Percent heptanol < 0.005 (HPLC) HO pH (1% solution) 5-8 HO O HO O Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06

225 nm < 0.1 NEW PRODUCTS 1. Anatrace measurement.

H300LA n-HEPTYL−β−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 75.92 [n-Heptyl−β−D-glucoside] 5 g 303.68 (Contains almost no alpha isomer) 25 g 1214.72

FW 278.4 [78617-12-6] C13H26O6 1 CMC (H2O) ~ 70 mM (1.9%) Purity ≥ 99% by HPLC analysis. Percent alpha < 0.1 (HPLC) Percent heptanol < 0.005 (HPLC) HO pH (1% solution) 5-8 HO O HO O Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

H301 n-HEPTYL−β−D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 60.52 [n-Heptyl−β−D-thioglucoside] 5 g 226.97

FW 294.4 [85618-20-8] C13H26O5S 25g 851.09 1 CMC (H2O) ~ 29 mM (0.85%) Gummy solid. Purity ≥ 99% by HPLC analysis. HO Percent alpha < 2 (HPLC) HO O pH (1% solution) 5-8 HO S Solubility in water at 0-5°C ≥ 10% OH Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25

PRODUCT INFOMATION 1. Anatrace measurement.

H301LA n-HEPTYL−β−D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 78.68 [n-Heptyl−β−D-thioglucoside] 5 g 314.69 (Contains almost no alpha isomer) 25 g 1258.76

FW 294.4 [85618-20-8] C13H26O5S 1 CMC (H2O) ~ 29 mM (0.85%) Gummy solid.

NEW PRODUCTS Purity ≥ 99% by HPLC analysis. Percent alpha < 0.1 (HPLC) HO pH (1% solution) 5-8 HO O HO S Solubility in water at 0-5°C > 10% OH Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 1. Anatrace measurement. PRODUCT LISTING

H320 n-HEXADECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 111.40 [n-Hexadecyl−β−D-maltoside] 5 g 417.77

FW 566.6 [98064-96-1] C28H54O11 25g 1566.62 1 CMC (H2O) ~ 0.0006 mM (0.00003%) Purity ≥ 97% pure by HPLC analysis. HO Percent alpha < 5 (HPLC) HO O Percent hexadecanol < 0.01 (HPLC) HO HO OH pH (0.1% solution) 5-8 O O HO O Solubility in water at 40°C ≥ 1.0% OH Conductance (0.1% solution) < 80 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.15

INDEX 225 nm < 0.3 NOTE: Heating may be required to dissolve detergent. 1. Anatrace estimate.

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Catalog No. Product Name/Specifications Size Price

H355 HEXADECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12

FW 320.00 [112-02-7] C19H42ClN 100 g 259.58 1 CMC (H2O) ~ 0.0032 mM (0.000102%) 3-4 Aggregation Number (H2O) ~ 61-84

N PRODUCT INFORMATION Purity ≥ 95% pure by titration. Cl pH (1% solution) 5.5-8.5 Solubility in water at 20°C ≥ 30% Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement. 2 H. Matsubara, T. Nakano, T. Matsuda, et al, Langmuir 21, 8131-8137 (2005). 3. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999). 4. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). NEW PRODUCTS H360 HEXAETHYLENE GLYCOL MONODECYL ETHER, ANALYTICAL GRADE 4 ml (1 ampule) 41.00

[C10E6 / Decyl hexaethylene glycol ether / Decyl hexaglycol 20 ml (2 ampules) 164.00 100 ml (10 ampules) 656.00

FW 422.60 [5168-89-8] C22H46O7 CMC 0.9 mM1 Aggregation Number1 73 Supplied as a 25% (w/w) aqueous solution under argon. Purity ≥ 99% by HPLC analysis. CH3(CH2)9O(CH2CH2O)6H Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 3-7 Conductance (10% solution) < 100 μS Solubility > 25% at room temperature 1. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).

H350 HEXAETHYLENE GLYCOL MONOOCTYL ETHER, ANAGRADE® 2 ml (1 ampule) 66.61

[C8E6] 10 ml (1 ampule) 266.43 50 ml (5 ampules) 1065.71

FW 394.5 [4440-54-4] C20H42O7 1 CMC (H2O) ~ 10 mM (0.39%) 1 Aggregation Number (H2O) ~ 32 Supplied as a 50% (w/w) solution under argon. Purity ≥ 99% by HPLC analysis. CH3(CH2)7O(CH2CH2O)6H Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 5-8 Conductance (10% solution) < 50 μS 1. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). INDEX

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Catalog No. Product Name/Specifications Size Price

H305 n-HEXYL−β−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.43 [n-Hexyl−β−D-glucoside] 5 g 233.71 25 g 934.84

FW 264.4 [59080-45-4] C12H24O6 1 CMC (H2O) ~ 250 mM (6.6%) Purity 99% by HPLC analysis. ≥ HO For molar volume check reference 2. HO O Percent alpha < 2 (HPLC) HO O Percent hexanol < 0.005 (HPLC) OH pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. 2. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).

H310 n-HEXYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 59.99 [n-Hexyl−β−D-maltoside] 5 g 224.95 NEW PRODUCTS 25 g 843.56

FW 426.4 [870287-95-9] C18H34O11 1 HO CMC (H2O) ~ 210 mM (8.9%) HO O Purity ≥ 99% by HPLC analysis. HO HO Percent alpha < 2 (HPLC) OH O O Percent hexanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS

PRODUCT LISTING Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement.

I1002 ISOPROPYL−β−D-THIOGALACTOPYRANOSIDE, ANAGRADE® 1 g 30.60 [IPTG / 1-Methylethyl-1-thio−β−D-galactopyranoside] 5 g 122.91 25 g 430.32

FW 238.31 [367-93-1] C9H18O5S White solid OH OH Purity ≥ 99% by HPLC analysis. O Conductance of a 10% solution < 40 μS Absorbance of a 1% solution: 340 nm < 0.04 HO S OH 280 nm < 0.04 INDEX 260 nm < 0.06 No dioxane detected by HPLC. PRECAUTIONS: Protect from light. Store frozen and keep dry. Warm to room temperature before opening.

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Catalog No. Product Name/Specifications Size Price

I1003 ISOPROPYL−β−D-THIOGALACTOPYRANOSIDE, ANALYTICAL GRADE 5 g 32.64 [IPTG / 1-Methylethyl-1-thio−β−D-galactopyranoside] 25 g 122.40 100 g 342.72

FW 238.31 [367-93-1] C9H18O5S White solid OH

OH PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. O Conductance of a 10% solution < 200 μS HO S Absorbance of a 1% solution: 340 nm < 0.1 OH 280 nm < 0.1 260 nm < 0.2 No dioxane detected by HPLC. PRECAUTIONS: Protect from light. Store frozen and keep dry. Warm to room temperature before opening.

I1003P ISOPROPYL−β−D-THIOGALACTOPYRANOSIDE, ANALYTICAL GRADE 5 g 38.36 [IPTG / 1-Methylethyl-1-thio−β−D-galactopyranoside] 25 g 150.00

Source: Plant 100 g 480.00 NEW PRODUCTS

FW 238.31 [367-93-1] C9H18O5S White solid OH OH Purity ≥ 99% by HPLC analysis. O Conductance of a 10% solution < 200 μS HO S Absorbance of a 1% solution: 340 nm < 0.1 OH 280 nm < 0.1 260 nm < 0.2 No dioxane detected by HPLC. PRECAUTIONS: Protect from light. Store frozen and keep dry. Warm to room temperature before opening.

L1000 LB BROTH, READY-MADE POWDER 250 g 16.00

Form: Off-white powder

pH (2.0%, H2O): 7.0 + 0.2 Contents: Casein Peptone: 10gm/L Yeast Extract: 5gm/L Sodium Chloride: 5gm/L To use: Dissolve 20gm powder per liter of purified water. For the cultivation of E. coli. Low salt formulation is ideal for culturing in the presence of salt-sensitive antibiotics.

IPTG is an inducer of the lac operon 1 and is often used in conjunction with a b-galactosidase substrate such as X-GAL (pg. 103) for determination of the lac phenotype in recombinant organisms. Some IPTG References: 1. Baim, S. B., Labow, A. J. Levine and Shenk, T., A chimeric mammalian transactivator based on the lac repressor that is

regulated by temperature and isopropyl-D-thiogalactopyranoside, Proc. Natl. Acad. Sci. USA 88, 5072-7056 (1991). INDEX 2. Pestov, D. G. and Lau, L. F., Genetic selection of groth-inhibitory sequences in mammalian cells. Proc. Natl. Acad. Sci. USA 91, 12549-12553 (1994). 3. Yanzhuang, L. and Lau, L. F., IPTG-Inducible Episomal Expression System for Exogenous Genes in Primate Cells, BioTechniques 28, 577-581 (2000).

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Catalog No. Product Name/Specifications Size Price

L360S LAPAO, SOL-GRADE® 1 g 50.96 [3-Dodecylamido-N,N'-dimethylpropyl amine oxide / 5 g 203.84 3-Laurylamido-N,N'-dimethylpropyl amine oxide] 25 g 815.36

FW 300.55 [61792-31-2] C17H36N2O2 1 CMC (H2O) ~ 1.56 mM (0.052%) 1 H O Aggregation Number (H2O) ~ 126 N N Purity ≥ 95% by HPLC analysis. O Purified to contain < 1000 μM of equivalent peroxide. Color: Pale Yellow pH (1% solution) 4-9 Solubility in water at 20oC ≥ 20% Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.2

PRODUCT INFOMATION 280 nm < 0.3 260 nm < 0.4 1. Personal communication from Professor Ronald S. Kaplan, Chicago Medical School. 2. C. Dahout-Gonzalez, G. Brandolin, E. Pebay-Peyroula, Acta. Cryst. D59, 2353-2355 (2003).

L1002 LITHIUM DODECYL SULFATE (LDS) 5 g 21.00 25 g 71.00

FW 272.33 [2044-56-6] CH3(CH2)11 OSO3Li NEW PRODUCTS Form: White crystalline powder

Assay (C12 by GC): ≥ 98% Absorbance (280nm, 3%, 1cm): < 0.1 Absorbance (230nm, 3%, 1cm): < 0.2 O O S OLi Loss on Drying: < 1% O Chloride (Cl): < 0.01% Copper (Cu): < 5 ppm Iron (Fe): < 1 ppm

PRODUCT LISTING Lead (Pb): < 5 ppm May be used for electrophoresis in cold conditions.

L1001 LURIA BROTH, READY-MADE POWDER 250 g 15.00

Form: Off-white powder

pH (2.5%, H2O): 7.0 + 0.2 Contents: Casein Peptone: 10gm/L Yeast Extract: 5gm/L Sodium Chloride: 10gm/L To use: Dissolve 25gm powder per liter of purified water. Autoclave. For the cultivation of E. coli. INDEX

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Catalog No. Product Name/Specifications Size Price

B2010 MAGENTA-GAL 50 mg 22.88 [5-Bromo-6-chloro-3-indolyl−β−D-galactopyranoside] 250 mg 91.52

FW 408.6 [93863-88-8] C H BrClNO H 14 15 6 N Cl OH OH Purity ≥ 95% pure by HPLC analysis. O PRODUCT INFORMATION Specific Rotation [a]24 (c=1.3, EtOH 96%) -44° ± 3° Br D O Melting Point: 187-191°C HO OH Solubility > 1% in EtOH IR spectrum conforms to specifications.

M319 MEGA-8, ANAGRADE® 1 g 31.78 [Octanoyl-N-methylglucamide] 5 g 127.13 25 g 508.53

FW 321.4 [85316-98-9] C15H31NO6 1,2 CMC (H2O) ~ 79 mM (2.5%) O NEW PRODUCTS Purity ≥ 99% by HPLC analysis. N OH pH (1% solution) 5-8 H OH Solubility in water at 0-5°C ≥ 20% H OH HO OH Conductance (10% solution) < 80 μS H H Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Y. Mine, K. Fukunaga, N. Maruoka, et al, J. Biosci. Bioeng. 90, 631-636 (2000). 3. Hanatani, M., et al, J. Biochem. 95, 1349-1353 (1984). 4. Hildreth, J. E. K., Biochem. J. 207, 363 (1982).

M325 MEGA-9, ANAGRADE® 1 g 31.78 (Nonanoyl-N-methylglucamide) 5 g 127.13 25 g 508.53

FW 335.5 [85261-19-4] C16H33NO6 1,2 CMC (H2O) ~ 25 mM (0.84%) O Purity ≥ 99% by HPLC analysis. pH (1% solution) 5-8 N OH Solubility in water at 5°C ≥ 5% H OH Conductance (5% solution) < 80 μS H OH HO OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H H Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement. 2. Hanatani, M., et al, J. Biochem. 95, 1349-1353 (1984). 3. Hildreth, J. E. K., Biochem. J. 207, 363 (1982). INDEX

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Catalog No. Product Name/Specifications Size Price

M320 MEGA-10, ANAGRADE® 1 g 31.78 [Decanoyl-N-methylglucamide] 5 g 127.13 25 g 508.53

FW 349.5 [85261-20-7] C17H35NO6 1,4 CMC (H2O) ~ 6-7 mM (0.21%) O Purity ≥ 99% by HPLC analysis. pH (0.3% solution) 5-8 N OH H Solubility in water at 0-5°C ≥ 0.3% OH Conductance (0.3% solution) < 40 μS H OH HO OH H Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 H Absorbance of a 0.3% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.1 1. Anatrace measurement.

PRODUCT INFOMATION 2. M. A. Churchward, R. H. Butt, J. C. Lang, et al, Proteome Sci. 3, 5 (2005). 3. J. M. Hierrezuelo, J. Aguiar, C. C. Ruiz, Langmuir 20, 10419-10426 (2004). 4. Hanatani, M., et al, J. Biochem. 95, 1349-1353 (1984). 5. Hildreth, J. E. K., Biochem. J. 207, 363 (1982).

M2500 MICROPIPETTES (25mL) 250 49.00 - glass

NEW PRODUCTS M110MT MMTS 100 mg 68.00 [Methyl methanethiosulfonate] 1 g 540.00 H C 3 S FW 126.20 [2949-92-0] C H O S 2 6 2 2 OOS Form: Light yellow liquid CH3 Boiling point: 69-70°C / 0.4mm

O110MT MTSL 10 mg 60.00 [(1-Oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) methanethiosulfonate] 100 mg 480.00

PRODUCT LISTING O CH3 FW 264.30 [N/A] C10H18NO3S2 S S Form: Light yellow powder O

Melting point: 107-108°C N O

ND195 NDSB-195 5 g 41.34 [Dimethylethylammoniumpropanesulfonate] 25 g 165.36 100 g 529.15 O FW 195.3 [160255-06-1] C7H17NO3S N S Solubility in water ≥ 10% O IR spectrum conforms to specifications. O Elemental analysis supplied with each lot (C,H,N). INDEX

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Catalog No. Product Name/Specifications Size Price

ND201 NDSB-201 25 g 20.14 [(3-1-Pyridino)-1-propane sulfonate / 100 g 64.45 1-(3-Sulfopropyl)-pyridinium hydroxide, inner salt] 500 g 257.79

FW 201.2 [15471-17-7] C H NO S O 8 11 3 N S

Solubility in water ≥ 10% PRODUCT INFORMATION O O IR spectrum conforms to specifications. Elemental analysis supplied with each lot (C,H,N).

ND211 NDSB-211 1 g 43.46 [Dimethyl(2-hydroxyethyl)ammonium-1-propanesulfonate] 5 g 173.84 25 g 695.36 O FW 211.3 [38880-58-9] C7H17NO4S HO N S Solubility in water ≥ 10% O IR spectrum conforms to specifications. O

Elemental analysis supplied with each lot (C,H,N). NEW PRODUCTS

ND221 NDSB-221 5 g 41.34 [3-(1-Methylpiperidinium)-1-propane sulfonate] 25 g 165.36 100 g 529.15 O FW 221.3 [160788-56-7] C H NO S 9 19 3 N S Solubility in water > 10% O O IR spectrum conforms to specifications. Elemental analysis supplied with each lot (C,H,N).

ND256 NDSB-256 5 g 41.34 [Dimethylbenzylammoniumpropanesulfonate] 25 g 165.36 100 g 529.15 O FW 257.4 [81239-45-4] C12H19NO3S N S Solubility in water 10% O ≥ O IR spectrum conforms to specifications. Elemental analysis supplied with each lot (C,H,N).

NIDP40 NONIDET P40 SUBSTITUTE (see Cat. # APND40, pg. 24) 500 mL 31.00 (Igepal CA-630 / [Octylphenoxy]polyethoxyethanol) 1 L 44.00

FW avg. 603 [2497-59-8] CMC (50mM Na+) ~ 0.05-0.3 mM1 1 Aggregation Number (H2O) ~ 100-155 (OCH CH ) OH Density: 1.060 + 0.005 2 2 9 Clear, slightly yellow-green viscous liquid. Chemically indistinguishable from Nonidet P40, which is no longer commercially available. 1. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html INDEX

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Catalog No. Product Name/Specifications Size Price

N324 n-NONYL−β−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 58.43 [n-Nonyl−β−D-glucoside] 5 g 233.71 25 g 934.84

FW 306.4 [69984-73-2] C15H30O6 1 2 1 CMC (H2O) ~ 6.5 mM (0.20%) CMC (0.15M NaCl) ~ 6 mM CMC (1M NaCl) ~ 3.5 mM Purity ≥ 99% by HPLC analysis. Percent alpha < 2 (HPLC) HO Percent nonanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02

PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. 2. H. Zer, M. Vink, S. Shochat, et al, Biochemistry 42, 728-738 (2003). 3. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997). 4. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997). 5. DeGrip, W. J. and Bovee-Geurts, P. H. M., Chem. Phys. Lipids 23, 312-325 (1979).

® NEW PRODUCTS N324LA n-NONYL−β−D-GLUCOPYRANOSIDE, ANAGRADE 1 g 84.24 [n-Nonyl−β−D-glucoside] 5 g 336.96 (Low alpha) 25 g 1347.84

FW 306.4 [69984-73-2] C15H30O6 1 2 1 CMC (H2O) ~ 6.5 mM (0.20%) CMC (0.15M NaCl) ~ 6 mM CMC (1M NaCl) ~ 3.5 mM Purity ≥ 99% by HPLC analysis. HO Percent alpha < 0.4 (HPLC) HO O Percent nonanol < 0.005 (HPLC) HO O

PRODUCT LISTING pH (1% solution) 5-8 OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. See N324 for references.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

N324S n-NONYL−β−D-GLUCOPYRANOSIDE, SOL-GRADE® 1 g 45.72 [n-Nonyl−β−D-glucoside] 5 g 182.88 25 g 731.56

FW 306.4 [69984-73-2] C15H30O6 1 2 1 CMC (H2O) ~ 6.5 mM (0.20%) CMC (0.15M NaCl) ~ 6 mM CMC (1M NaCl) ~ 3.5 mM

Purity ≥ 97% pure by HPLC analysis. PRODUCT INFORMATION Percent alpha < 5 (HPLC) HO Percent nonanol < 0.05 (HPLC) HO O pH (1% solution) 4-9 HO O Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 100 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2

1. See N324 for references. NEW PRODUCTS

N330 n-NONYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 58.60 [n-Nonyl−β−D-maltoside] 5 g 219.75 25 g 824.08

FW 468.5 [106402-05-5] C21H40O11 1 CMC (H2O) ~ 6 mM (0.28%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 55 Purity 99% by HPLC analysis. ≥ HO Percent alpha < 2 (HPLC) O Percent nonanol < 0.005 (HPLC) HO HO HO pH (1% solution) 5-8 OH O O Solubility in water at 0-5°C ≥ 20% HO O Conductance (10% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

N335 n-NONYL−β−D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Nonyl−β−D-thioglucoside] 5 g 307.99 25 g 1154.93

FW 322.4 [98854-15-0] C15H30O5S CMC (water:methanol1) ~ 2.9 mM2 (0.093%)

Purity ≥ 99% by HPLC analysis. HO Percent alpha < 2 (HPLC) HO O 1 pH (0.05% solution ) 5-8 HO S Solubility in water at 0-5°C1 ≥ 0.05% OH Conductance (0.05% solution1) < 40 μS Percent fluorescence due to a 0.05% detergent solution1 at 345 nm < 10 Absorbance of a 0.05% detergent solution1: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 PRODUCT INFOMATION 1. Solvent: (1:1) v/v methanol : water. 2. Anatrace measurement.

N350 n-NONYL−β−D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 (n-Nonyl−β−D-thiomaltoside) 5 g 307.99 25 g 1154.93

FW 484.6 [148565-55-3] C21H40O10S 1 CMC (H2O) ~ 3.2 mM (0.15%) NEW PRODUCTS HO Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 2 (HPLC) HO HO OH pH (1% solution) 5-8 O O Solubility in water at 0-5°C ≥ 20% HO S OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 260 nm < 0.2 PRODUCT LISTING 1. Anatrace measurement. 2. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997).

N150 NOPOL-FOS™, ANAGRADE® 1 g 132.91 [6,6-Dimethylbicyclo[3.1.1]hept-2-ene-2-ethylphosphocholine] 5 g 531.63 25 g 2126.55

FW 331.4 [869660-84-4] C16H30NO4P CMC (H O) ~ 42.5 mM1 (1.4%) 2 O Purity ≥ 99% by HPLC analysis. O PO pH (1% solution) 5-8 N O Solubility in water at 0-5oC ≥ 20% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.2 260 nm < 0.3 INDEX 1. Anatrace measurement.

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Catalog No. Product Name/Specifications Size Price

O355 OCTADECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12

FW 348.1 [112-03-8] C21H46ClN 100 g 259.58 1 CMC (H2O) < 0.03 mM (0.001%) Purity ≥ 95% pure by titration. N pH (0.05% solution) 5.5-8.5 Cl PRODUCT INFORMATION Solubility in water at 20°C ≥ 0.05% Absorbance of a 0.05% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement. 2. N. O. McHedlov-Petrossyan, N. A. Vodolazkaya, A. A. Kornienko, et al, Langmuir 21, 7090-7096 (2005). 3. Y. Jiang, W. Qian, J.W. Hawes, J. P. Walsh, J. Biol. Chem. 275, 34092-34099 (2000). 4. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999). 5. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). NEW PRODUCTS O330 OCTAETHYLENE GLYCOL MONODODECYL ETHER, ANAGRADE® 4 ml (1 ampule) 79.70

[C12E8 / Dodecyl octaethylene glycol ether / Dodecyl octaglycol / 20 ml (2 ampules) 318.80 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol] 100 ml (10 ampules) 1275.20

FW 538.77 [3055-98-9] C28H58O9 1,2 CMC (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 0.09 mM (0.0048%) 2 Aggregation Number (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 90-120 Supplied as a 25% (w/w) aqueous solution under argon.

Purity ≥ 99% by HPLC analysis. CH3(CH2)11O(CH2CH2O)8H Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 3-7 Conductance (10% solution) < 100 μS Solubility > 25% at room temperature 1. LeMaire, M., Kwee, S., Andersen, J. P. and Miller, J. V., Eur. J. Biochem. 129, 525-532, (1983). 2. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).

O330A OCTAETHYLENE GLYCOL MONODODECYL ETHER, ANALYTICAL GRADE 4 ml (1 ampule) 41.00

[C12E8 / Dodecyl octaethylene glycol ether / Dodecyl octaglycol / 20 ml (2 ampules) 164.00 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol] 100 ml (10 ampules) 656.00

FW 538.77 [3055-98-9] C28H58O9 1,2 CMC (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 0.09 mM (0.0048%) 2 Aggregation Number (0.01 M TES, pH 7.5, 0.05 M NaCl, 0.1 mM CaCl2) ~ 90-120 Supplied as a 25% (w/w) aqueous solution under argon. Purity ≥ 97% pure by HPLC analysis.

pH (1% solution) 3-7 CH3(CH2)11O(CH2CH2O)8H Conductance (10% solution) < 500 μS Solubility > 25% at room temperature Caution: Contains organic peroxides. INDEX 1. LeMaire, M., Kwee, S., Andersen, J. P. and Miller, J. V., Eur. J. Biochem. 129, 525-532, (1983). 2. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).

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Catalog No. Product Name/Specifications Size Price

O312 n-OCTYL−β−D-GALACTOPYRANOSIDE, ANAGRADE® 1 g 53.47 [n-Octyl−β−D-galactoside] 5 g 200.50 25 g 751.87

FW 292.4 [40427-75-6] C14H28O6 1 CMC (H2O) ~ 29.5 mM (0.86%) Purity ≥ 99% by HPLC analysis. OH OH Percent alpha < 2 (HPLC) O Percent octanol < 0.005 (HPLC) HO O pH (0.5% solution) 5-8 OH Solubility in water at 0-5°C ≥ 0.5% Conductance (0.5% solution) < 10 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.02 280 nm < 0.04 PRODUCT INFOMATION 260 nm < 0.06 225 nm < 0.1 1. Stevenson, D. E., Stanley, R. A. and Furneaux, R. H., Biotech. Bioeng. 42, 657-666, (1993).

O311 n-OCTYL−β−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 29.00 [n-Octyl−β−D-glucoside] 5 g 108.75 25 g 407.81

NEW PRODUCTS FW 292.4 [29836-26-8] C14H28O6 1 2 CMC (H2O) ~ 18-20 mM (0.53%) CMC (0.1 M NaCl) ~ 23.4 mM 1 Aggregation Number (H2O) ~ 27-100 Purity ≥ 99% by HPLC analysis. For Molar volume check reference 4. Percent alpha < 2 (HPLC) HO Percent octanol < 0.005 (HPLC) HO O pH (1% solution) 5-8 HO O Solubility in water at 0-5°C ≥ 20% OH

PRODUCT LISTING Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Lorber, B., Bishop, J. B. and DeLucas, L. J., Biochim. Biophys. Acta 1023, 254-265 (1990). 2. Chattopadhyay, A. and London, E., Anal. Biochem. 139, 408-412 (1984). 3. Womack, M. D., Kendall, D. A. and MacDonald, R. C., Biochim. Biophys. Acta 733, 210-215 (1983). 4. Brown, G. M., Dubreuil, P., Ichhaporia, F. M. and Desnoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970). 5. Conlan, S. and Bayley, H., Biochem. 42, 9453-9465 (2003). 6. Fanucci, G. E., Lee, J. Y., and Cafiso, D. S., Biochemistry 42, 13106-13112 (2003).

Anatrace offers five different grades of octyl glucoside and six different grades of dodecyl maltoside. INDEX If you need a special grade of any detergent, ask us.

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Catalog No. Product Name/Specifications Size Price

O311HA n-OCTYL−α−D-GLUCOPYRANOSIDE, ANAGRADE® 1 g 69.00 [n-Octyl−α−D-glucoside] 5 g 276.00 25 g 1104.00

FW 292.4 [29781-80-4] C14H28O6 CMC (H O) ~ .010 mM1 (0.0002%) 2 HO PRODUCT INFORMATION Purity ≥ 99% by HPLC analysis. HO O Percent alpha > 98% (HPLC) HO OH Percent octanol < 0.005 (HPLC) O pH (0.1% solution) 5-8 Solubility in water at 20°C ≥ 0.1% Heating may be required to dissolve detergent Conductance (0.1% solution) < 50 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.1% detergent solution: 340 nm < 0.02 280 nm < 0.04

260 nm < 0.06 NEW PRODUCTS 225 nm < 0.10 1. Brown, G. M., Dubreuil P., Ichhaporia, F. M., and Deshoyers, J. E., Canadian J. Chem. 48, 2525-2531 (1970).

O311S n-OCTYL−β−D-GLUCOPYRANOSIDE, SOL-GRADE® 1 g 25.73 [n-Octyl−β−D-glucoside] 5 g 96.49 25 g 361.83

FW 292.4 [29836-26-8] C14H28O6 1 2 CMC (H2O) ~ 18-20 mM (0.53%) CMC (0.1 M NaCl) ~ 23.4 mM 1 Aggregation Number (H2O) ~ 27-100 Purity ≥ 97% pure by HPLC analysis. Percent alpha < 5 (HPLC) HO Percent octanol < 0.05 (HPLC) HO O pH (1% solution) 4-9 HO O Solubility in water at 20°C ≥ 20% OH Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 1. See O311 for references.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

O311T n-OCTYL-d17−β−D-GLUCOPYRANOSIDE 100 mg 214.25

[n-Octyl-d17−β−D-glucoside, tail deuterated] 250 mg 482.06 500 mg 915.92

FW 309.5 [129522-81-2] C14D17H11O6 1 CMC (H2O) ~ 20 mM Purity ≥ 97% pure by HPLC analysis. Percent alpha < 2 (HPLC) HO Percent octanol < 0.05 (HPLC) O D HO D D D D D D D pH (1% solution) 5-8 HO O OH D Solubility in water at 20°C ≥ 10% D D D D D D D D Conductance (1% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.15 PRODUCT INFOMATION 260 nm < 0.15 225 nm < 0.5 1. CMC value for the undeuterated compound.

O311D n-OCTYL-d17−β−D-GLUCOPYRANOSIDE-d7 100 mg 508.66

[n-Octyl-d17−β−D-glucoside-d7] 250 mg 1144.85 500 mg 2060.07

NEW PRODUCTS FW 316.5 [869666-57-9] C14D24H4O6 1 CMC (H O) ~ 19 mM D OH 2 D D D D D D D D D D Purity ≥ 97% pure by HPLC analysis. D O O D D Percent alpha < 2 (HPLC) OH D D D D D D D D Percent octanol < 0.05 (HPLC) OH D Conductance (1% solution) < 40 μS D OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.1 280 nm < 0.15

PRODUCT LISTING 260 nm < 0.15 225 nm < 0.5 1. CMC value for the undeuterated compound.

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

O310 n-OCTYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 62.05 [n-Octyl−β−D-maltoside] 5 g 248.18 25 g 992.74

FW 454.4 [82494-08-4] C20H38O11 CMC (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 19.5 mM1 (0.89%) 1 Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 47 PRODUCT INFORMATION

Purity ≥ 99% by HPLC analysis. HO Percent alpha < 2 (HPLC) HO O Percent octanol < 0.005 (HPLC) HO HO OH pH (1% solution) 5-8 O O Solubility in water at 0-5°C ≥ 20% HO O OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04

260 nm < 0.06 NEW PRODUCTS 225 nm < 0.1 1. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).

O310S n-OCTYL−β−D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 49.64 [n-Octyl−β−D-maltoside] 5 g 198.55 25 g 794.19

FW 454.4 [82494-08-4] C20H38O11 CMC (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 19.5 mM1 (0.89%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)1 ~ 47

Purity ≥ 98% pure by HPLC analysis. HO Percent alpha < 5 (HPLC) HO O Percent octanol < 0.05 (HPLC) HO HO OH pH (1% solution) 4-9 O O Solubility in water at 20°C ≥ 20% HO O OH Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University).

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

O314 n-OCTYL−β−D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 25.16 [n-Octyl−β−D-thioglucoside] 5 g 100.63 25 g 402.52

FW 308.4 [85618-21-9] C14H28O5S 1 CMC (H2O) ~ 9 mM (0.28%)

Purity ≥ 99% by HPLC analysis. HO Percent alpha < 2 (HPLC) HO O pH (0.5% solution) 5-8 HO S Solubility in water at 0-5°C ≥ 0.8% OH Conductance (0.5% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.1 280 nm < 0.2 260 nm < 0.25 PRODUCT INFOMATION 1. Saito, S. and Tsuchiya, T., Biochem. J. 222, 829-832 (1984). 2. Wenk, M. R. and Seelig, J., Biophys. J. 73, 2565-2574 (1997).

O314LA n-OCTYL−β−D-THIOGLUCOPYRANOSIDE, ANAGRADE® 1 g 34.94 [n-Octyl−β−D-thioglucoside] 5 g 139.78 (Contains almost no alpha isomer) 25 g 559.10

FW 308.4 [85618-21-9] C14H28O5S NEW PRODUCTS 1 CMC (H2O) ~ 9 mM (0.28%) Purity ≥ 99% by HPLC analysis. HO Percent alpha < 0.1 (HPLC) HO O pH (0.5% solution) 5-8 HO S Solubility in water at 0-5°C ≥ 0.8% OH Conductance (0.5% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 0.5% detergent solution: 340 nm < 0.1 280 nm < 0.2 PRODUCT LISTING 260 nm < 0.25 1. See O314 for references.

O320 n-OCTYL−β−D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Octyl−β−D-thiomaltoside] 5 g 307.99 25 g 1154.93

FW 470.6 [148616-91-5] C20H38O10S CMC (H O) ~ 8.5 mM1 (0.40%) 2 HO Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 2 (HPLC) HO HO OH pH (1% solution) 5-8 O O Solubility in water at 0-5°C ≥ 20% HO S Conductance (10% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05

INDEX 280 nm < 0.15 260 nm < 0.2 1. Anatrace measurement. 2. Mechref, Y. and Rassi, Z. E., J. Chromatography 757, 263-273 (1997).

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Catalog No. Product Name/Specifications Size Price

P340 PENTAETHYLENE GLYCOL MONODECYL ETHER, ANAGRADE® 2 ml (1 ampule) 62.84

[C10E5 / Decyl pentaethylene glycol ether / Decylpentaglycol / 10 ml (1 ampule) 251.35 3,6,9,12,15-Pentaoxapentacosan-1-ol] 50 ml (5 ampules) 1005.39

FW 378.55 [23244-49-7] C20H42O6 1 CMC (H2O) ~ 0.81mM (0.031%) 2 Aggregation Number (H2O) ~ 73 PRODUCT INFORMATION

Supplied as a 50% (w/w) solution under argon. CH3(CH2)9O(CH2CH2O)5H Purity ≥ 99% by HPLC analysis. Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 5-8 Conductance (10% solution) < 50 μS 1. Borchardt, J. K., TODAY’S CHEMIST AT WORK 5, 10, 20-24, November 1996. 2. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000).

P350 PENTAETHYLENE GLYCOL MONOOCTYL ETHER, ANAGRADE® 2 ml (1 ampule) 62.84 NEW PRODUCTS [C8E5 / Octyl pentaethylene glycol ether / Octylpentaglycol / 10 ml (1 ampule) 251.35 3,6,9,12,15-Pentaoxatricosan-1-ol] 50 ml (5 ampules) 1005.39

FW 350.5 [19327-40-3] C18H38O6 CMC (0.1 M NaCl) ~ 7.1 mM1 (0.25%) Supplied as a 50% (w/w) solution under argon. Purity ≥ 99% by HPLC analysis. CH3(CH2)7O(CH2CH2O)5H Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 5-8 Conductance (10% solution) < 50 μS 1. Eisele, J. and Vulliez-Le Normand, B., Anal. Biochem. 208, 241-243, (1993).

P300 PLURONIC® F-68 100 g 11.06 [Polyoxyethylene-polyoxypropylene Block Copolymer / 500 g 44.22 . Methyl-oxirane, polymer with oxirane, (C3H6O C2H4O)x / Poloxamer 188]

FW ~ 8400 [9003-11-6] EO78PO30EO78 1,2 CMC (H2O, 27°C) ~ 17.9 mM 1. Alexandridis, P., Holzwarth, J. F. and Hatton, T. A., Macromolecules 27, 2414-2425 (1994). 2. Alexandridis, P., Athanassiou, V., Fukuda, S. and Hatton, T. A., Langmuir 10, 2604-2612 (1994).

CH3

HO(CH2CH2O)x(CH2CH2O)y(CH2CH2O)zH x ~ 78 y ~ 30 z ~ 78

P305 PLURONIC® F-127 100 g 11.06 [Polyoxyethylene-polyoxypropylene Block Copolymer / 500 g 44.22 . Methyl-oxirane, polymer with oxirane, (C3H6O C2H4O)x / Poloxamer 407]

FW ~ 12600 [9003-11-6] EO100PO65EO100 1,2 CMC (H2O, 19.5°C) ~ 3.97 mM

1. Alexandridis, P., Holzwarth, J. F. and Hatton, T. A., Macromolecules 27, 2414-2425 (1994). INDEX 2. Alexandridis, P., Athanassiou, V., Fukuda, S. and Hatton, T. A., Langmuir 10, 2604-2612 (1994).

CH3

HO(CH2CH2O)x(CH2CH2O)y(CH2CH2O)zH x ~ 100 y ~ 65 z ~ 100

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Catalog No. Product Name/Specifications Size Price

P5008 PMAL™-C8 1 g 144.56 [Poly (maleic anhydride-alt-1-decene) substituted with 5 g 578.24 3-(Dimethylamino) propylamine] [869856-84-8] (C H O N ) 19 36 3 2 n O O MW ~ 18,500 o Solubility in water at 20 C ≥ 10% n Absorbance of a 0.1% detergent solution: 340 nm < 0.1 O NH 280 nm < 0.3 260 nm < 0.5 N

IR spectrum conforms to specifications. No maleic anhydride present by thermographic analysis PRODUCT INFOMATION 1. Nagy, J. K., Hoffmann, A. K., Keyes, M. H., Gray, D. N., Oxenoid, K. and Sanders, C. R., FEBS Letters 501, 115-120 (2001). 2. Gorzelle, B. M., Hoffmann, A. K., Keyes, M. H., Gray, D. N., Ray, D. G., Sanders, C. R., J. Am. Chem. Soc. 124, Amphipols Can Support the Activity of a Membrane Enzyme, 11594-11595 (2002).

P5010 PMAL™-C10 1 g 144.56 [Poly (maleic anhydride-alt-1-dodecene) substituted with 5 g 578.24 3-(Dimethylamino) propylamine] NEW PRODUCTS [869856-88-2] (C H O N ) 21 40 3 2 n O O MW ~ 18,000 o Solubility in water at 20 C ≥ 10% n Absorbance of a 0.1% detergent solution: 340 nm < 0.1 O NH 280 nm < 0.3 260 nm < 0.5 IR spectrum conforms to specifications. N No maleic anhydride present by thermographic analysis 1. See P5008 for references. PRODUCT LISTING

P5012 PMAL™-C12 1 g 144.56 [Poly (maleic anhydride-alt-1-tetradecene) substituted with 5 g 578.24 3-(Dimethylamino) propylamine] [869857-14-7] (C H O N ) 23 44 3 2 n O O MW ~ 12,000 o Solubility in water at 20 C ≥ 10% n Absorbance of a 0.1% detergent solution: 340 nm < 0.1 O NH 280 nm < 0.3 260 nm < 0.5 IR spectrum conforms to specifications. N No maleic anhydride present by thermographic analysis 1. See P5008 for references. INDEX

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P5016 PMAL™-C16 1 g 144.56 [Poly (maleic anhydride-alt-1-octadecene) substituted with 5 g 578.24

3-(Dimethylamino) propylamine] [869857-16-9] (C27H52O3N2)n

O O MW ~ 39,000-65,000 Solubility in water at 20oC ≥ 1% n PRODUCT INFORMATION Absorbance of a 0.1% detergent solution: 340 nm < 0.1 O NH 280 nm < 0.3 260 nm < 0.5 N IR spectrum conforms to specifications. No maleic anhydride present by thermographic analysis 1. See P5008 for references.

P400 POLYETHYLENE GLYCOL 400 (PEG 400) 500 ml 29.00 (PEG 400) 1 L 43.00

FW 380-420 [25322-68-3] NEW PRODUCTS Form: Clear colorless viscous liquid Density (d 20/4): 1.12-1.13 Refractive Index (n 20/d): 1.465-1.469 Viscosity (20°C): 102-120 mpas

P6000 POLYETHYLENE GLYCOL 6000, FLAKES 500 gm 26.00 (PEG 6000) 1 kg 45.00 5 kg 206.00 FW 5400-6600 [25322-68-2]

Form: White or slightly yellowish flakes

Solubility (50%, Hot H2O): Clear and colorless Melting Point: Approx. 60°C

1. Lerman, L.S., Proc. Natl. Acad. Sci. 68, 1886 (1971). 2. Harrison, B. and Zimmerman, S.B., Anal. Biochem. 158, 307 (1986).

P8000F POLYETHYLENE GLYCOL 8000, FLAKES 500 gm 26.00 (PEG 8000) 1 kg 45.00 5 kg 206.00 [25322-68-3] Form: White flakes Approx. M.W.=8,000 1. Lerman, L.S., Proc. Natl. Acad. Sci. 68, 1886 (1971). 2. Harrison, B. and Zimmerman, S.B., Anal. Biochem. 158, 307 (1986).

P8000P POLYETHYLENE GLYCOL 8000, POWDER 500 gm 26.00 (PEG 8000) 1 kg 45.00 5 kg 206.00

Molecular Weight Range: 7000-9000 INDEX Form: White to off-white powder

Solubility (50%, Hot H2O): Clear and colorless Melting Point: 60-64°C 1. Lerman, L.S., Proc. Natl. Acad. Sci. 68, 1886 (1971). 2. Harrison, B. and Zimmerman, S.B., Anal. Biochem. 158, 307 (1986).

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Catalog No. Product Name/Specifications Size Price

P310 2-PROPYL-1-PENTYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 71.76 5 g 287.04

FW 455.5 [869668-28-0] C20H39O11 25 g 1148.16 1 CMC (H2O) ~ 42.5 mM (1.9%) Purity ≥ 99% by HPLC analysis. HO Percent alpha < 10% HO O Percent (2-propyl-1-pentanol) < 0.005% HO HO OH O pH (1% solution) 5-8 O HO O o Solubility in water at 0-5 C ≥ 20% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 PRODUCT INFOMATION 225 nm < 0.1 1. Anatrace measurement.

S2000 L-(+)-SELENOMETHIONINE, ANALYTICAL GRADE 250 mg 40.25 [(S)-2-Amino-4-(methylseleno)butyric acid] 1 g 134.16 5 g 536.64

FW 196.11 [3211-76-5] C5H11NO2Se

NEW PRODUCTS Purity ≥ 98% pure by HPLC analysis. O Specific Rotation [a]20 (C=0.5 in 2N HCl) +18.5° ± 1.5° D Se Melting Point: 258-262° OH Solubility: Soluble in water, slightly soluble in methanol NH2 IR spectrum conforms to specifications. PRODUCT LISTING

References on the use of Selenomethionine : 1. Yang, W., Hendrickson, W. A., Crouch, R. J. and Satow, Y., Structure of Ribonuclease H Phased at 2 A Resolution by MAD Analysis of the Selenomethionyl Protein, Science 249, 1398-1405 (1990).

2. Jornot, L. and Junod, A. F. Differential regulation of glutathione peroxidase by selenomethionine and hyperoxia in endothelial cells, Biochem. J. 306, 581-587 (1995).

3. Leahy, D. J., Hendrickson, W. A., Aukhil, I. and Erickson, H. P., Structure of a Fibronectin Type III Domain fromTenascin Phased by MAD Analysis of the Selenomethionyl Protein, Science 258, 987-991 (1992).

INDEX 4. Crane, B. R., Siegel, L. M. and Getzoff, E. D., Sulfite Reductase Structure at 1.6 A: Evolution and Catalysis for Reduction of Inorganic Anions, Science 270, 59-67 (1995).

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Catalog No. Product Name/Specifications Size Price

S1010 SODIUM CHOLATE, ANAGRADE® 5 g 34.69 [3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid, monosodium salt] 25 g 130.09 100 g 416.28

FW 430.6 [361-09-1] C24H39O5Na 1 2 CMC (pH 9.0) ~ 9.5 mM (0.41%) CMC (pH 7.5) ~ 14 mM O 3 Aggregation Number (H2O) ~ 2.0-4.8 PRODUCT INFORMATION ONa Purity ≥ 99% by HPLC analysis. OH pH (1% solution) 5-8 H Solubility in water at 20°C ≥ 40% Absorbance of a 1% detergent solution: 400 nm < 0.02 H H 340 nm < 0.04 HO OH 280 nm < 0.08 260 nm < 0.1 1. Brito, R. M. M and Vaz, W. L. C., Anal. Biochem 152, 250-255 (1986). 2. Vendittis, E., Paumbo, G., Parlata, G. and Borchini, U., Anal. Biochem. 115, 278-286 (1981). 3. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). 4. Iwaki, M., Giotta, L., Akinsiku, A. O., Schagger, H., Fisher, N., Breton, J., and Rich, P. R., Biochem. 42, NEW PRODUCTS 11109-11119 (2003).

S1010S SODIUM CHOLATE, SOL-GRADE® 25 g 16.00 [3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid, monosodium salt] 100 g 51.20 500 g 204.80

FW 430.6 [361-09-1] C24H39O5Na CMC (pH 9.0) ~ 9.5 mM1 (0.41%) CMC (pH 7.5) ~ 14 mM2 (0.60%) O 3 Aggregation Number (H2O) ~ 2.0-4.8 ONa Purity ≥ 98% pure by HPLC analysis. OH pH (1% solution) 5-8 Solubility in water at 20°C ≥ 40% H Absorbance of a 1% detergent solution: 400 nm < 0.04 H H 340 nm < 0.08 HO OH 280 nm < 0.12 260 nm < 0.2 1. See S1010 for references.

S300 SODIUM DODECANOYL SARCOSINE, ANAGRADE® 1 g 33.66 [Sodium lauroyl sarcosine / Sarkosyl / N-Methyl-N-(1-oxododecyl)-glycine, sodium salt] 5 g 126.23 25 g 473.34

FW 293.4 [137-16-6] C15H29NO3Na 1 CMC (H2O) ~ 14.4 mM (0.42%) O Purity ≥ 99% by HPLC analysis. N Percent lauric acid < 0.1 NaO pH (10% solution) 7-9 O Solubility in water at 20°C ≥ 10% Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.06 INDEX 260 nm < 0.08 1. Anatrace measurement.

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Catalog No. Product Name/Specifications Size Price

S300S SODIUM DODECANOYL SARCOSINE, SOL-GRADE® 5 g 24.99 [Sodium lauroyl sarcosine / N-Methyl-N-(1-oxododecyl)-glycine, sodium salt] 25 g 93.72 100 g 318.63

FW 293.4 [137-16-6] C15H28NNaO3 1 CMC (H2O) ~ 14.4 mM (0.42%) O Purity ≥ 96% pure by HPLC analysis. N Percent lauric acid < 4 NaO pH (10% solution) 7-9 O Solubility in water at 20°C ≥ 10% Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.15 1. Anatrace measurement. PRODUCT INFOMATION S1001 SODIUM DODECYL SULFATE, ANAGRADE® 100 g 22.00 [SDS / Sulfuric acid monododecyl ester sodium salt] 500 g 88.00 1 kg 140.80

FW 288.38 [151-21-3] C12H26O4SNa 1 2 CMC (pH 7.5) ~ 2.6 mM (0.075%) CMC (H2O) ~ 8.27 mM (0.24%) 3 Aggregation Number (H2O) ~ 62-101 O O Purity 99% by HPLC analysis. S ≥ ONa NEW PRODUCTS Absorbance of a 1% detergent solution: 260 nm < 0.04 O 280 nm < 0.02 Chloride < 0.1% Phosphate < 0.0005% Lead < 0.0005% Copper < 0.0005% RNase, DNase activity None detected PRECAUTIONS: Store at room temperature. Protect from moisture. 1. Brito, R. M. M and Vaz, W. L. C., Anal. Biochem 152, 250-255 (1986).

PRODUCT LISTING 2. Mukerjee, P. and Mysels, K. J., Critical Micelle Concentrations of Aqueous Surfactant Systems, Nat’l. Std. Data Service, National Bureau of Standards, Washington, D.C. (1971). 3. Anatrace Measurement.

S110MT SODIUM (2-SULFONATOETHYL) METHANETHIOSULFONATE 100 mg 33.55 [MTSES / Methanesulfonothioic acid, sodium salt] 500 mg 134.20 1 g 241.56

FW 242.28 [1950-85-2] C3H7NaO5S3

Solubility in water at 0-5°C ≥ 10% O O S Negatively charged reagent that reacts very rapidly S S ONa and specifically with cysteine groups. O O Half-life (pH 7.0, 20°C) ~ 370.0 minutes2 1. Akabas, M. H., Stauffer, D. A., Xu, M. and Karlin, A., Science 258, 307-310 (1992). 2. Karlin, A. and Akabas, M. H., in Methods Enzymol. 293, 123-136 (1998). 3. Stauffer, D. A. and Karlin, A., Biochem. 33, 6840-6849 (1994). 3. Sobszak, I. and Lolkema, J. S., Biochem. 42, 9789-9796 (2003). INDEX

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Catalog No. Product Name/Specifications Size Price

S2033 SODIUM TAUROCHOLATE, ANAGRADE® 1 g 31.08 [3α,7α,12α-Trihydroxy-5β-cholan-24-oic acid N-(2-sulfoethyl) amide] 5 g 129.48 25 g 453.18

FW 537.7 [145-42-6] C26H44NO7SNa CMC ~ 3-11 mM1 (0.16-0.59%) O O 1 S ONa Aggregation Number (H2O) ~ 4 PRODUCT INFORMATION O Purity 95% pure by HPLC analysis. OH N ≥ H pH (1% solution) 4-8 Solubility in water at 20°C ≥ 10% H Absorbance of a 1% detergent solution: 400 nm < 0.02 H H 340 nm < 0.04 HO OH 280 nm < 0.08 260 nm < 0.1 1. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html

®

S350 SUCROSE MONODODECANOATE, ANAGRADE 1 g 48.14 NEW PRODUCTS [β-D-Fructopyranosyl−α−D-glucopyranoside monododecanoate / 5 g 192.57 Lauroyl sucrose / Dodecanoyl sucrose] 25 g 770.27

FW 524.61 [25339-99-5] C24H44O12 O 1 CMC (H2O) ~ 0.3 mM (0.016%) O HO Purity ≥ 97% pure by HPLC analysis. O pH (1% solution) 5-8 HO O HO HO O Solubility in water at 0-5°C ≥ 20% OH OH HO Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.08 260 nm < 0.10 NOTE: Sucrose monododecanoate (S350) contains three different isomers which differ in the location of the ester linkage. Esterification takes place at each of the primary alcohols in the sucrose molecule. 1. Makino, H., et al, Agric. Biol. Chem. 4, 319 (1983). 2. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997).

T1000 TCEP 5 g 205.00 [Tris (carboxyethyl) phosphine hydrochloride] 10 g 321.00 OH OH

O P O FW 286.65 [51805-45-9] C9H15O6P . HCl Form: White powder Assay (titration): ≥ 99% HO O Storage: + 4°C INDEX

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Catalog No. Product Name/Specifications Size Price

T360 n-TETRADECYL-N,N-DIMETHYLAMINE-N-OXIDE, ANAGRADE® 1 g 38.23 [TDAO / N,N-Dimethyl-1-tetradecanamine-N-oxide] 5 g 152.92 25 g 611.69

FW 257.46 [3332-27-2] C16H35NO 1 1 CMC (H2O) ~ 0.29 mM (0.0075%) CMC (0.1 M NaCl) ~ 0.024 mM Purity ≥ 99% by HPLC analysis. Chromatographically purified to contain O < 600 mM of equivalent peroxide. N pH (1% solution) 4-9 Solubility in water at 20oC ≥ 1% Conductance (1% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06 PRODUCT INFOMATION 260 nm < 0.08 1. Anatrace measurement.

T305 n-TETRADECYL-N,N-DIMETHYLGLYCINE, ANAGRADE® 1 g 50.96 5 g 203.84

FW 299.4 [2601-33-4] C18H37NO2 25 g 815.36 1 CMC (H2O) ~ 0.034 mM (0.0010%)

NEW PRODUCTS Purity ≥ 99% by HPLC analysis. O pH (1% solution) 5-8 N O Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 100 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.08 260 nm < 0.10 1. Anatrace measurement. PRODUCT LISTING T315 n-TETRADECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 500 mg 64.85 [n-Tetradecyl−β−D-maltoside / 1 g 116.74 Tetradecyl 4-O−α−D-glucopyranosyl−β−D-glucopyranoside] 5 g 466.96

FW 538.6 [18449-82-6] C26H50O11 CMC (H O) ~ 0.01 mM1 (0.00054%) 2 HO Purity ≥ 99% by HPLC analysis. HO O Percent alpha < 2 (HPLC) HO HO OH Percent tetradecanol < 0.005 (HPLC) O O pH (1% solution) 5-8 HO O OH Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.06

INDEX 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement.

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Catalog No. Product Name/Specifications Size Price

T315S n-TETRADECYL−β−D-MALTOPYRANOSIDE, SOL-GRADE® 500 mg 57.65 [n-Tetradecyl−β−D-maltoside / 1 g 103.76 Tetradecyl 4-O−α−D-glucopyranosyl−β−D-glucopyranoside] 5 g 415.06

FW 538.6 [18449-82-6] C26H50O11 1 CMC (H O) ~ 0.01 mM (0.00054%) PRODUCT INFORMATION 2 HO Purity ≥ 98% pure by HPLC analysis. HO O Percent alpha < 5 (HPLC) HO HO OH Percent tetradecanol < 0.05 (HPLC) O O pH (1% solution ) 4-9 HO O OH Solubility in water at 20°C ≥ 20% Conductance (10% solution) < 100 μS Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1

225 nm < 0.2 NEW PRODUCTS 1. Anatrace measurement.

T355 TETRADECYLTRIMETHYLAMMONIUM CHLORIDE 5 g 20.28 25 g 81.12

FW 279.90 [4574-04-3] C17H38ClN 100 g 259.58 1 CMC (H2O) ~ 0.032 mM (0.0009%) 2-3 Aggregation Number (H2O) ~ 62-81 N Purity ≥ 95% pure by titration. Cl pH (1% solution) 5.5-8.5 Solubility in water at 20°C ≥ 30% Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.08 1. Anatrace measurement. 2. Neugebauer, J. M., Methods Enzymol. 182, 239-253 (1990). 3. Maillet, J.-B., Lachet, V., and Coveney, P. V., Phys. Chem. Chem. Phys. 1, 5277-5290 (1999).

T350 TETRAETHYLENE GLYCOL MONOOCTYL ETHER, ANAGRADE® 2 ml (1 ampule) 60.42

[C8E4 / Octyl tetraethylene glycol ether / Octyltetraglycol / 10 ml (1 ampule) 241.68 3,6,9,12-Tetraoxaeicosan-1-ol] 50 ml (5 ampules) 966.72

FW 306.45 [19327-39-0] C16H34O5 CMC (0.1M NaCl) ~ 8 mM1 (0.25%) 1 Aggregation Number (H2O) ~ 82 Supplied as a 50% (w/w) aqueous solution under argon. Purity ≥ 99% by HPLC analysis. CH3(CH2)7O(CH2CH2O)4H Chromatographically purified to contain < 500 μM of equivalent peroxide. pH (5% solution) 5-8 INDEX Conductance (10% solution) < 50 μS 1. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). 2. Cortes, D. M. and Perozo, E., Biochem. 36, 10343-10352 (1997).

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Catalog No. Product Name/Specifications Size Price

T323 n-TRIDECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 500 mg 64.85 [n-Tridecyl−β−D-maltoside] 1 g 116.74 5 g 466.96

FW 524.6 [93911-12-7] C25H48O11 1 1 CMC (H2O) ~ 0.033 mM (0.0017%) CMC (0.15 mM NaCl) ~ 0.024 mM (0.0013%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 186 Purity ≥ 99% by HPLC analysis. HO Percent alpha < 2 (HPLC) HO O Percent tridecanol < 0.005 (HPLC) HO HO OH pH (1% solution) 5-8 O O Solubility in water at 0-5°C ≥ 20% HO O Conductance (10% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10

PRODUCT INFOMATION Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.06 260 nm < 0.1 225 nm < 0.2 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract.

T323LA n-TRIDECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 500 mg 91.00 NEW PRODUCTS [n-Tridecyl−β−D-maltoside] 1 g 160.71 (Contains almost no alpha isomer) 5 g 642.83

FW 524.6 [93911-12-7] C25H48O11 1 1 CMC (H2O) ~ 0.033 mM (0.0017%) CMC (0.15 mM NaCl) ~ 0.024 mM (0.0013%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 186

Purity ≥ 99% by HPLC analysis. HO Percent alpha < 0.2 (HPLC) O Percent tridecanol < 0.005 (HPLC) HO HO PRODUCT LISTING HO pH (1% solution) 5-8 OH O O Solubility in water at 0-5°C ≥ 20% HO O Conductance (10% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.04 280 nm < 0.06 260 nm < 0.1 225 nm < 0.2 See T323 for references. INDEX

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Catalog No. Product Name/Specifications Size Price

T323S n-TRIDECYL−β−D-MALTOPYRANOSIDE, SOL-GRADE® 500 mg 57.65 [n-Tridecyl−β−D-maltoside] 1 g 103.76 5 g 415.06

FW 524.6 [93911-12-7] C25H48O11 1 1 CMC (H2O) ~ 0.033 mM (0.0017%) CMC (0.15 mM NaCl) ~ 0.024 mM (0.0013%) 2 Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5) ~ 186 PRODUCT INFORMATION

Purity ≥ 97% pure by HPLC analysis. HO Percent alpha < 5 (HPLC) O Percent tridecanol < 0.05 (HPLC) HO HO HO pH (1% solution) 4-9 OH O O Solubility in water at 20°C ≥ 20% HO O Conductance (10% solution) < 100 μS OH Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1

225 nm < 0.2 NEW PRODUCTS

See T323 for references.

T110MT [2-(TRIMETHYLAMMONIUM)ETHYL] METHANE THIOSULFONATE BROMIDE 100 mg 33.55 [MTSET] 500 mg 134.20 1 g 241.56

FW 278.24 [91774-25-3] C6H16BrNO2S2

O Solubility in water at 0-5°C ≥ 10% S Positively charged reagent that reacts very rapidly S N Br and specifically with cysteine groups. O Half-life (pH 7.0, 20°C) ~ 11.2 minutes1 Half-life (pH 6.0, 20°C) ~ 55.0 minutes1 1. Karlin, A. and Akabas, M. H., in Methods Enzymol. 293, 123-136 (1998). 2. Stauffer, D. A. and Karlin, A., Biochem. 33, 6840-6849 (1994). 3. Sobszak, I. and Lolkema, J. S., Biochem. 42, 9789-9796 (2003).

T370 TRIPAO 500 mg 79.05 [3-(3-butyl-3-phenylheptanamido)-N,N-dimethylpropan-1-amine oxide] 1 g 126.48 5 g 452.88

FW: 362.55 [N/A] C32H28N2O2 CMC 4.5 mM1 O Purity ≥ 95% by HPLC analysis. pH (1% solution) 4-9 N N H O Solubility in water at 20oC ≥ 2% Conductance (10% solution) < 500 μS Absorbance of a 1% detergent solution: 340 nm < 0.2

280 nm < 0.3 INDEX

T1001 TRITON® X-100 500 ml 26.00

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Catalog No. Product Name/Specifications Size Price [α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy-poly(oxy-1,2-ethanediyl)]

FW avg. 647 [9002-93-1] t-Oct-C6H4-(OCH2CH2)XOH, x = 9-10 1-4 CMC (H2O) ~ 0.010 - 0.016% (w/v) (0.015%) 5 Aggregation Number (H2O) ~ 75-165 Clear viscous liquid Density: 1.070 (OCH2CH2)10OH IR spectrum conforms to specifications. PRECAUTIONS: Store at room temperature. Protect from moisture. 1. Vendittis, E., Paumbo, G., Parlata, G., and Borchini, U., Anal.Biochem. 115, 278-286 (1981). 2. Ross, S. and Oliver, J. P., J. Phys.Chem. 63, 1671-1674 (1959). 3. Mankovich, A. M., J. Amer. Oil Chem. Soc. 41, 449-452 (1964). 4. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983). 5. LeMaire, M., Champeil, P. and Moller, J. V., Biochimica et Biophysica Acta 1508, 86-111 (2000). PRODUCT INFOMATION T1002 TRITON® X-114 500 ml 26.00 [α-[(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy-poly(oxy-1-2-ethanediyl)]

FW avg. 536 [9036-19-5] t-Oct-C6H4-(OCH2CH2)XOH, x = 7-8 1 CMC (H2O) ~ 0.009% (w/v) (0.011%) Clear viscous liquid IR spectrum conforms to specifications. (OCH2CH2)8OH

NEW PRODUCTS PRECAUTIONS: Store at room temperature. Protect from moisture. 1. Rosenthal, K. S. and Koussale F., Anal. Chem. 55, 1115-1117 (1983).

T1003 TWEEN® 20 500 ml 26.00 (Polyoxyethylene(20)sorbitan monolaurate) Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate HO(H2CH2CO)w (OCH2CH2)xOH FW avg. 1228 [9005-64-5] (OCH2CH2)yOH

PRODUCT LISTING 1 O CMC (H2O) ~ 0.059 mM (0.0072%)

Clear liquid O(CH2CH2O)zR IR spectrum conforms to specifications. Sum of w+x+y+z=20 R=C H C(O) PRECAUTIONS: Store at room temperature. Protect from moisture. 11 23 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Wu, G., Kulmacz, R. J., and Tsai, A., Biochemistry 42, 13772-13777 (2003).

T1005 TWEEN® 40 1 gal 120.00 (Polyoxyethylene sorbitan monolaurate) HO(H2CH2CO)w [9005-66-7] (OCH2CH2)xOH (OCH CH ) OH O 2 2 y Form: Yellow liquid Storage: Ambient O(CH2CH2O)zR INDEX Sum of w+x+y+z=20 R=C15H31C(O)

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Catalog No. Product Name/Specifications Size Price

T1006 TWEEN® 60 1 gal 44.00 (Polyoxyethylene sorbitan monooleate)

HO(H2CH2CO)w [9005-67-8] (OCH2CH2)xOH

(OCH CH ) OH O 2 2 y PRODUCT INFORMATION

O(CH2CH2O)zR Sum of w+x+y+z=20 R=C17H35C(O)

T1004 TWEEN® 80 500 ml 26.00 [Polyoxyethylene(80)sorbitan monolaurate / Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan mono-9-octadecenoate]

HO(H2CH2CO)w FW avg. 1310 [9005-65-6] (OCH2CH2)xOH 1 CMC (H2O) ~ 0.012 mM (0.0016%) (OCH CH ) OH NEW PRODUCTS 2 O 2 2 y Aggregation Number (H2O) ~ 58 Golden-yellow viscous liquid O(CH2CH2O)zR IR spectrum conforms to specifications. Sum of w+x+y+z=20 R=C H C(O) PRECAUTIONS: Store at room temperature. Protect from moisture. 17 33 1. Helenius, A., McCauslin, D. R., Fries, E. and Tanford, C., Methods Enzymol. 56, 743-749 (1979). 2. Black, Shaun D.: http://psyche.uthct.edu/shaun/SBlack/detergnt.html

U360 n-UNDECYL-N,N-DIMETHYLAMINE-OXIDE, ANAGRADE® 1 g 50.96 [Undecyldimethylamine-N-oxide / UDAO / N,N-Dimethyl-1-undecamine-N-oxide 5 g 203.84 25 g 815.36

FW 215.38 [15178-71-9] C13H29NO 1 CMC (H2O) ~ 3.21 mM (0.069%) O Purity ≥ 99% by HPLC analysis. N Purified to contain < 500 μM of equivalent peroxide. pH (1% solution) 4-9 Solubility in water at 20°C ≥ 30% Conductance (10% solution) < 200 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.06 260 nm < 0.08 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

U300 n-UNDECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 67.13 [n-Undecyl−β−D-maltoside] 5 g 268.55 25 g 1074.20

FW 496.6 [253678-67-0] C23H44O11 1 CMC (H2O) ~ 0.59 mM (0.029%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 71

Purity ≥ 99% by HPLC analysis. HO Percent alpha < 2 (HPLC) O Percent undecanol < 0.005 (HPLC) HO HO HO pH (1% solution) 5-8 OH O O Solubility in water at 0-5°C ≥ 20% HO O Conductance (10% solution) < 40 μS OH Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 PRODUCT INFOMATION 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract. 3. Ostermeier, C., Harrenga, A., Ermler, U., and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997). 4. Zhang, H., Kurisu, G., Smith, J. L., and Cramer, W. A., PNAS 100, No. 9, 5160-5163 (2003). NEW PRODUCTS U300HA n-UNDECYL−α−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 118.72 [n-Undecyl−α−D-maltoside / Undecyl maltoside] 5 g 474.88 (Alpha isomer) 25 g 1899.52

FW 496.6 [168037-13-6] C23H44O11 1 CMC (H2O) ~ 0.58 mM (0.029%) HO

Purity ≥ 99% by HPLC analysis. HO O Percent alpha > 94 (HPLC) HO HO OH O Percent < 0.005 (HPLC) O HO PRODUCT LISTING pH (1% solution) 5-8 OH O Solubility in water at 0-5°C ≥ 20% Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.10 260 nm < 0.15 225 nm < 0.25 1. Anatrace measurement. INDEX

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Catalog No. Product Name/Specifications Size Price

U300LA n-UNDECYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 92.71 [n-Undecyl−β−D-maltoside] 5 g 370.85 (Low alpha) 25g 1483.41

FW 496.6 [253678-67-0] C23H44O11 1 CMC (H2O) ~ 0.59 mM (0.029%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 71 PRODUCT INFORMATION

Purity ≥ 99% by HPLC analysis. HO Percent alpha < 0.2 (HPLC) HO O Percent undecanol < 0.005 (HPLC) HO HO pH (1% solution) 5-8 OH O O o Solubility in water at 0-5 C ≥ 20% HO O Conductance (10% solution) < 40 μS OH Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 225 nm < 0.1 NEW PRODUCTS See U300 for references.

U300S n-UNDECYL−β−D-MALTOPYRANOSIDE, SOL-GRADE® 1 g 53.71 [(n-Undecyl−β−D-maltoside] 5 g 214.82 25 g 859.29

FW 496.6 [253678-67-0] C23H44O11 1 CMC (H2O) ~ 0.59 mM (0.029%) Aggregation Number (100 mM NaCl, 20 mM HEPES pH 7.5)2 ~ 71

Purity ≥ 97% pure by HPLC analysis. HO Percent alpha < 5 (HPLC) O Percent undecanol < 0.05 (HPLC) HO HO HO pH (1% solution) 4-9 OH O O Solubility in water at 20°C ≥ 20% HO O Conductance (10% solution) < 100 μS OH Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.1 260 nm < 0.1 225 nm < 0.2 See U300 for references. INDEX

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Catalog No. Product Name/Specifications Size Price

U342 n-UNDECYL−β−D-THIOMALTOPYRANOSIDE, ANAGRADE® 1 g 82.13 [n-Undecyl−β−D-thiomaltoside] 5 g 307.99 25 g 1154.93

FW 512.7 [148565-57-5] C23H44O10S CMC (H O) ~ 0.21 mM1 (0.011%) 2 HO 2 Aggregation Number (H2O) ~ 106 HO O Purity ≥ 98% pure by HPLC analysis. HO HO OH Percent alpha < 2 (HPLC) O O pH (1% solution) 5-8 HO S Solubility in water at 20°C ≥ 10% OH Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.05 280 nm < 0.15 PRODUCT INFOMATION 260 nm < 0.2 1. Anatrace measurement. 2. Measurement obtained in collaboration with Professor Mark Foster (University of Akron) under an experimental services contract.

U310 ω-UNDECYLENYL−β−D-MALTOPYRANOSIDE, ANAGRADE® 1 g 69.68 [ω-Undecylenyl−β−D-maltoside] 5 g 278.72 25 g 1114.88 NEW PRODUCTS FW 494.6 [N/A] C23H42O11 HO CMC (H O) ~ 1.2 mM1 (0.059%) 2 HO O HO HO OH O O Purity 99% by HPLC analysis. ≥ HO O Percent alpha < 2 (HPLC) OH Percent w-undecylenyl alcohol < 0.005 (HPLC) pH (1% solution) 5-8 Solubility in water at 0-5°C ≥ 20%

PRODUCT LISTING Conductance (10% solution) < 40 μS Percent fluorescence due to a 0.1% detergent solution at 345 nm < 10 Absorbance of a 1% detergent solution: 340 nm < 0.02 280 nm < 0.04 260 nm < 0.06 1. Anatrace measurement. 2. Anatrace measurement in collaboration with Professor R. M. Garavito (Michigan State University). 3. Ostermeier, C., Harrenga, A., Ermler, U. and Michel, H., Proc. Natl. Acad. Sci. USA 94, 10547-10553 (1997).

Notes: INDEX

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Catalog No. Product Name/Specifications Size Price

U1000 UREA, ANAGRADE® 1 kg 47.00

FW 60.06 [57-13-6] NH2CONH2 Form: White crystalline powder Assay: ≥ 99.5% Identity: By IR O PRODUCT INFORMATION Spectral Ratios (260 nm, 6M, 1 cm): < 0.055 Spectral Ratios (280 nm, 6M, 1 cm): < 0.044 H2NNH2 Melting Point: 132-135°C Insoluble Matter < 0.01% Residue on Ignition: < 0.01% Conductivity: < 15 μmhos/cm Cyanate: None detected Chloride (Cl): < 0.0005%

Sulfate (SO4): < 0.001% Copper (Cu): < 0.5 ppm

Iron (Fe): < 0.5 ppm NEW PRODUCTS Lead (Pb): < 0.5 ppm DNase (endo): Passes test RNase: Passes test Protease: Passes test

B2000 X-GAL, ANAGRADE® 1 g 50.96 [5-Bromo-4-chloro-3-indoyl−β−D-galactopyranoside] 5 g 203.84 25 g 815.36

FW 408.6 [7240-90-6] C14H15BrClNO6 Purity 99% by HPLC analysis. H ≥ OH N Specific Rotation[a]20 (c=1, H O:DMF 1:1) -61° ± 3° OH D 2 O Solubility > 2% in DMF Br HO O Cl IR spectrum conforms to specifications. OH

B2050 X-GAL-IPTG, Solution 1.5 ml 29.00

Form: Clear, light yellow solution H N HO X-Gal Concentration: 10 mg/ml OH OH ≥ O HO Br O IPTG Concentration: ≥ 8 mg/ml HO O Cl OH HO S OH To Use: Spread 100 μl X-Gal-IPTG solution on a Petri dish or mix 100 μl X-Gal-IPTG solution with 25 ml of molten agar. INDEX

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Catalog No. Product Name/Specifications Size Price

B2020 X-GLUC, CHA SALT, ANAGRADE® 25 mg 30.16 [5-Bromo-4-chloro-3-indolyl−β−D-glucuronic acid , 100 mg 95.68 cyclohexyl ammonium salt] 500 mg 386.05

FW 521.8 [114162-64-0] C H BrClN O NH 20 26 2 7 3 H N Purity ≥ 99% by HPLC analysis. O O HO O Br Specific Rotation [a]20 (c=1, H O: DMF 1:1) -90.5° ± 3° HO O Cl D 2 OH Solubility ≥ 2% in DMF IR spectrum conforms to specifications.

B2030 X-GLUC, Na SALT, ANAGRADE® 25 mg 57.20

PRODUCT INFOMATION [5-Bromo-4-chloro-3-indolyl−β−D-glucuronic acid, sodium salt] 100 mg 171.60 500 mg 643.50

FW 444.6 [129541-41-9] C14H12BrClNO7Na

+ H Purity ≥ 99% by HPLC analysis. Na N O O 20 HO O Br Specific Rotation [a] D (C=1, H2O: DMF 1:1) -107° ± 3° HO O Cl Solubility ≥ 2% in DMF OH IR spectrum conforms to specifications. NEW PRODUCTS PRODUCT LISTING INDEX

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Kits To select the optimum detergent or combination of detergents and reagents for a particular membrane protein usually requires testing of many detergents. Note also that the detergent which is suitable for extraction may not be useful for storage of the PRODUCT INFORMATION purified protein or for studies conducted on the purified protein. To aid in the selec- tion process, Anatrace offers a wide variety of solid and solution based detergent kits. Listed below are nonionic and ionic Master Detergent Kits which contain one gram of each detergent. Following the master kits are several smaller kits which likewise contain one gram of the specified detergents. Please Note: Kit pricing represents a substantial discount. NEW PRODUCTS D399-NDK NONIONIC MASTER DETERGENT KIT 2489.43 Contains one gram of each of the detergents listed below:

ANAMEG®-7 n-Hexyl−β−D-maltopyranoside MEGA-8 n-Octyl D-maltopyranoside Big CHAP −β− MEGA-9 n-Nonyl D-maltopyranoside Big CHAP, Deoxy −β− MEGA-10 n-Decyl−α−D-maltopyranoside ™ 2-Propyl-1-pentyl−β−D- CYGENT 1-3 n-Decyl−β−D-maltopyranoside maltopyranoside CYGLU®-3 n-Undecyl−α−D-maltopyranoside CYGLU®-4 n-Undecyl−β−D-maltopyranoside Hexaethylene glycol monooctyl n-Dodecyl−α−D-maltopyranoside ether (C8E6) (50% w/w) ® CYMAL -1 n-Dodecyl−β−D-maltopyranoside Octaethylene glycol monododecyl CYMAL®-2 n-Tridecyl−β−D-maltopyranoside ether (C12E8) (25% w/w) ® CYMAL -3 n-Tetradecyl−β−D-maltopyranoside Pentaethylene glycol monodecyl CYMAL®-4 n-Hexadecyl−β−D-maltopyranoside ether (C10E5) (50% w/w) ® CYMAL -5 ω-Undecylenyl−β−D-maltopyranoside Pentaethylene glycol monooctyl CYMAL®-6 ether (C8E5) (50% w/w) ® n-Heptyl−β−D-thioglucopyranoside CYMAL -7 Tetraethylene glycol monooctyl n-Octyl−β−D-thioglucopyranoside ether (C E ) (50% w/w) 2,6-Dimethyl-4-heptyl−β− n-Nonyl−β−D-thioglucopyranoside 8 4 D-maltopyranoside n-Decyl−β−D-thioglucopyranoside n-Octyl−β−D-galactopyranoside n-Hexyl−β−D-glucopyranoside n-Octyl−β−D-thiomaltopyranoside Sucrose monododecanoate n-Heptyl−β−D-glucopyranoside n-Nonyl−β−D-thiomaltopyranoside Dimethyldecylphosphine oxide n-Octyl−α−D-glucopyranoside n-Decyl−β−D-thiomaltopyranoside Dimethyloctylphosphine oxide n-Octyl−β−D-glucopyranoside n-Undecyl−β−D-thiomaltopyranoside n-Tetradecyl-N,N-dimethylamine- n-Nonyl−β−D-glucopyranoside n-Dodecyl−β−D-thiomaltopyranoside N-oxide (TDAO) n-Decyl−β−D-glucopyranoside n-Dodecyl-N,N-dimethylamine- n-Dodecyl−β−D-glucopyranoside

N-oxide (DDAO) INDEX

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D399-IDK IONIC MASTER DETERGENT KIT 2816.42 Contains one gram of each of the detergents listed below:

ANZERGENT®3-8 FOS-CHOLINE®-13 n-Decyl-N,N-dimethylglycine ANZERGENT®3-10 FOS-CHOLINE®-14 n-Dodecyl-N,N-dimethylglycine ANZERGENT®3-12 FOS-CHOLINE®-15 n-Tetradecyl-N,N-dimethylglycine ANZERGENT®3-14 FOS-CHOLINE®-16 n-Dodecyl−β−iminodipropionic acid, FOS-CHOLINE®-ISO-9 C-DODECAFOS™ disodium salt FOS-CHOLINE®-ISO-11 n-Dodecyl−β−iminodipropionic acid, C-OCTAFOS™ FOS-CHOLINE®-ISO-11-6U ® monosodium salt CHAPS FOS-CHOLINE -UNSAT-11-10 Deoxycholic acid, sodium salt CHAPSO FOS-MEA®-8 ® Sodium cholate CYCLOFOS™-2 FOS-MEA -10 ® Sodium dodecanoyl sarcosine

PRODUCT INFOMATION ™ FOS-MEA -12 CYCLOFOS -3 Sodium dodecyl sulfate CYCLOFOS™-4 FOSFEN™-9 Sodium Taurocholate CYCLOFOS™-5 ™ CYCLOFOS™-6 NOPOL-FOS CYCLOFOS™-7 PMAL™-C8 PMAL™-C10 CYPFOS™-3 PMAL™-C12 FOS-CHOLINE®-8 PMAL™-C16 ® NEW PRODUCTS FOS-CHOLINE -9 Decyltrimethylammonium chloride FOS-CHOLINE®-10 Dodecyltrimethylammonium chloride FOS-CHOLINE®-11 Hexadecyltrimethylammonium chloride FOS-CHOLINE®-12 Octadecyltrimethylammonium chloride Tetradecyltrimethylammonium chloride

D399-CE ALKYL POLYOXYETHYLENE (CXEY) DETERGENT KIT 161.74

Kit contains Hexaethylene glycol monooctyl ether (C8E6) (50% w/w solution), PRODUCT LISTING Octaethylene glycol monododecyl ether (C12E8) (25% w/w solution), Pentaethylene

glycol monodecyl ether (C10E5) (50% w/w solution) and Tetraethylene glycol

monooctyl ether (C8E4) (50% w/w solution) ampuled and stored under Argon. D399-ANZ ANZERGENT® DETERGENT KIT 39.00 Kit contains ANZERGENT® 3-8, 3-10, 3-12 and 3-14. D399-C14 CYMAL® DETERGENT KIT (1-4) 197.91 Kit contains CYMAL®-1, 2, 3 and 4. D399-F812 FOS-CHOLINE® DETERGENT KIT (8-12) 406.98 Kit contains FOS-CHOLINE® 8, 9, 10, 11 and 12. D399-FM812 FOS-MEA® DETERGENT KIT 239.23 Kit contains FOS-MEA®-8, 10 and 12. D399-G GLUCOPYRANOSIDE DETERGENT KIT 191.26 Kit contains n-Hexyl−β−D-glucopyranoside, n-Heptyl−β−D-glucopyranoside, n-Octyl−β−D-glucopyranoside, n-Nonyl−β−D-glucopyranoside, n-Decyl−β−D- glucopyranoside, and n-Dodecyl D-glucopyranoside.

INDEX −β−

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D399-HA HIGH ALPHA DETERGENT KIT 213.70 Kit contains n-Decyl−α−D-maltopyranoside, n-Dodecyl−α−D-maltopyranoside, and n-Undecyl−α−D-maltopyranoside. D399-ION IONIC DETERGENT KIT 80.09 Kit contains Sodium cholate, n-Dodecyl-N,N-dimethylglycine, n-Decyl-N,N- dimethylglycine, Sodium dodecanoyl sarcosine, and Deoxycholic acid, Sodium salt. PRODUCT INFORMATION D399-M611 MALTOPYRANOSIDE DETERGENT KIT (6-11) 181.18 Kit contains n-Hexyl−β−D-maltopyranoside, n-Octyl−β−D-maltopyranoside, n-Nonyl- β-D-maltopyranoside, n-Decyl−β−D-maltopyranoside and n-Undecyl−β−D- maltopyranoside. D399-M1216 MALTOPYRANOSIDE DETERGENT KIT (12-16) 244.73 Kit contains n-Dodecyl−β−D-maltopyranoside, n-Tridecyl−β−D-maltopyranoside, n-Tetradecyl−β−D-maltopyranoside and n-Hexadecyl−β−D-maltopyranoside. D399-MG MEGA DETERGENT KIT 57.21 Kit contains MEGA-8, 9 and 10. NEW PRODUCTS D399-MSF MISCELLANEOUS FOS DETERGENT KIT 327.43 Kit contains FOS-CHOLINE®-ISO-9, NOPOL-FOS™, C-DODECAFOS™ and CYPFOS™-3. D399-MSFU MISCELLANEOUS FOS-CHOLINE® DETERGENT KIT 245.36 Kit contains FOS-CHOLINE®-ISO-11, FOS-CHOLINE®-ISO-11-6U and FOS-CHOLINE®-UNSAT-11-10. D399-OX OXIDE DETERGENT KIT 64.92 Kit contains Dimethyldecylphosphine oxide, n-Tetradecyl-N,N-dimethylamine-N- oxide (TDAO) and n-Dodecyl-N,N-dimethylamine-N-oxide (DDAO). D399-PM816 PMAL™ DETERGENT KIT (8-16) 346.94 Kit contains PMAL™-C8, PMAL™-C10, PMAL™-C12 and PMAL™-C16. D399-POP POPULAR DETERGENT KIT 228.39 Kit contains CHAPS, CYMAL®-5, FOS-CHOLINE®-12, n-Decyl−β−D-maltopyranoside, n-Dodecyl−β−D-maltopyranoside and n-Octyl−β−D-glucopyranoside. D399-QUAT QUATERNARY DETERGENT KIT 29.00 Kit contains Decyltrimethylammonium chloride, Dodecyltrimethylammonium chloride, Hexadecyltrimethylammonium chloride, Octadecyltrimethylammonium chloride, and Tetradecyltrimethylammonium chloride. D399-TG THIOGLUCOPYRANOSIDE DETERGENT KIT 149.96 Kit contains n-Heptyl−β−D-thioglucopyranoside, n-Octyl−β−D-thioglucopyranoside, n-Nonyl−β−D-thioglucopyranoside and n-Decyl−β−D-thioglucopyranoside. D399-TM THIOMALTOPYRANOSIDE DETERGENT KIT 246.39 Kit contains n-Octyl−β−D-thiomaltopyranoside, n-Nonyl−β−D-thiomaltopyranoside, n-Decyl−β−D-thiomaltopyranoside, n-Dodecyl−β−D-thiomaltopyranoside and n-Undecyl−β−D-Thiomaltopyranoside. INDEX

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Soluble Detergent Kits Save Time and Money Anatrace detergents are now available in kits as ampuled 10% solutions stored under argon. These solution kits cost a fraction of the one gram detergent kits, making the selection of the “right” detergent for extraction of a membrane protein less expensive. The master kit listed below contains nearly every detergent offered by Anatrace that possesses sufficient solubility to prepare a 10% solution. DSOL-MK SOLUTION MASTER DETERGENT KIT 1017.00 Contains one ampule (one ml of a 10% solution) of each detergent listed below:

ANAMEG®-7 2,6-Dimethyl-4-heptyl−β−D- n-Undecyl−α−D-maltopyranoside maltopyranoside n-Undecyl−β−D-maltopyranoside ANAPOE®-20 n-Dodecyl D-maltopyranoside PRODUCT INFOMATION 2-Propyl-1-pentyl maltopyranoside −α− ANAPOE®-35 n-Dodecyl−β−D-maltopyranoside ANAPOE®-58 FOS-CHOLINE®-8 n-Tridecyl−β−D-maltopyranoside ANAPOE®-80 FOS-CHOLINE®-9 n-Tetradecyl−β−D-maltopyranoside ® FOS-CHOLINE®-10 ANAPOE -C10E6 ® n-Heptyl−β−D-thioglucopyranoside ® FOS-CHOLINE -11 ANAPOE -C10E9 ® FOS-CHOLINE®-12 n-Octyl−β−D-thiomaltopyranoside ANAPOE -C12E8 ® FOS-CHOLINE®-13 n-Nonyl−β−D-thiomaltopyranoside ANAPOE -C12E9 ® FOS-CHOLINE®-14 n-Decyl−β−D-thiomaltopyranoside ANAPOE -C12E10 ANAPOE®-C E FOS-CHOLINE®-15 n-Undecyl−β−D-thiomaltopyranoside

NEW PRODUCTS 13 8 ANAPOE®-X-100 FOS-CHOLINE®-16 n-Dodecyl−β−D-thiomaltopyranoside ANAPOE®-X-114 FOS-CHOLINE®-ISO-9 ® n-Decyl-N,N-dimethylglycine ANAPOE®-X-305 FOS-CHOLINE -ISO-11 n-Dodecyl-N,N-dimethylglycine ANAPOE®-X-405 FOS-CHOLINE®-ISO-11-6U FOS-CHOLINE®-UNSAT-11-10 n-Dodecyl−β−iminodipropionic acid, ANZERGENT® 3-8 ® disodium salt ANZERGENT® 3-10 FOS-MEA -8 ANZERGENT® 3-12 FOS-MEA®-10 n-Dodecyl−β−iminodipropionic acid, monosodium salt ANZERGENT® 3-14 ™ FOSFEN -9 Sodium dodecanoyl sarcosine Big CHAP MEGA-8 Hexaethylene glycol monooctyl ether (C E ) Big CHAP, deoxy 8 6 PRODUCT LISTING NOPOL-FOS™ Octaethylene glycol monododecyl ether (C E ) CHAPS 12 8 Pentaethylene glycol monodecyl ether (C E ) CHAPSO PMAL™-C8 10 5 Tetraethylene glycol monooctyl ether (C E ) PMAL™-C10 8 4 Deoxycholic acid, sodium salt ™ Sodium cholate PMAL -C12 Sucrose monododecanoate C-DODECAFOS™ Decyltrimethylammonium chloride Dimethyldecylphosphine oxide CYCLOFOS™-2 Dodecyltrimethylammonium chloride n-Tetradecyl-N,N-dimethylamine-N- CYCLOFOS™-3 Hexadecyltrimethylammonium chloride oxide (TDAO) CYCLOFOS™-4 Octadecyltrimethylammonium chloride n-Dodecyl-N,N-dimethylamine-N- CYCLOFOS™-5 Tetradecyltrimethylammonium chloride oxide (DDAO) CYCLOFOS™-6 n-Hexyl−β−D-glucopyranoside CYCLOFOS™-7 n-Heptyl−β−D-glucopyranoside CYGLU®-3 n-Octyl−β−D-glucopyranoside CYMAL®-1 n-Nonyl−β−D-glucopyranoside CYMAL®-2 n-Hexyl D-maltopyranoside CYMAL®-3 −β− n-Octyl D-maltopyranoside CYMAL®-4 −β− n-Nonyl D-maltopyranoside CYMAL®-5 −β−

INDEX n-Decyl D-maltopyranoside CYMAL®-6 −α− n-Decyl D-maltopyranoside CYMAL®-7 −β− CYPFOS™-3

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Additional kits containing detergents as 10% aqueous solutions are listed below: DSOL-ANAP1 ANAPOE® MASTER KIT (1 mL size) 30.50 Kit contains 1 mL of all 15 ANAPOE® detergents DSOL-ANP10 ANAPOE® MASTER KIT (10 mL size) 83.00 Kit contains 10 mL of all 15 ANAPOE® detergents PRODUCT INFORMATION ® DSOL-ANPC ANAPOE CyEx KIT (10 mL size) 51.50 ® ® ® Kit contains 10 mL of ANAPOE -35, ANAPOE -58, ANAPOE -C10E6, ® ® ® ® ANAPOE -C10E9, ANAPOE -C12E8, ANAPOE -C12E9, ANAPOE -C12E10, ® and ANAPOE -C13E8. DSOL-ANPT ANAPOE® TRITON/TWEEN KIT (10 mL size) 30.50 Kit contains 10 mL of ANAPOE®-X-100, ANAPOE®-X-114, ANAPOE®-X-305, ANAPOE®-X-405, ANAPOE®-20, and ANAPOE®-80. DSOL-ANZ ANZERGENT® DETERGENT KIT 30.50 Kit contains ANZERGENT® 3-8, 3-10, 3-12 and 3-14.

DSOL-BILE BILE ACID-BASED DETERGENT KIT 62.00 NEW PRODUCTS Kit contains CHAPS, CHAPSO, Sodium cholate, Big CHAP, Big CHAP, deoxy and Deoxycholic acid, sodium salt. DSOL-CY25 CYCLOFOS™ DETERGENT KIT (2-5) 83.00 Kit contains CYCLOFOS™-2, 3, 4 and 5. DSOL-C14 CYMAL® DETERGENT KIT (1-4) 62.00 Kit contains CYMAL®-1, 2, 3 and 4. DSOL-C57 CYMAL® DETERGENT KIT (5-7) 72.50 Kit contains CYMAL®-5, 6 and 7. DSOL-F812 FOS-CHOLINE® DETERGENT KIT (8-12) 72.50 Kit contains FOS-CHOLINE®-8, 9, 10, 11 and 12. DSOL-F1316 FOS-CHOLINE® DETERGENT KIT (13-16) 72.50 Kit contains FOS-CHOLINE®-13, 14, 15 and 16. DSOL-G GLUCOPYRANOSIDE DETERGENT KIT 62.00 Kit contains n-Hexyl−β−D-glucopyranoside, n-Heptyl−β−D-glucopyranoside, n-Octyl−β−D-glucopyranoside and n-Nonyl−β−D-glucopyranoside. DSOL-HA HIGH ALPHA DETERGENT KIT 72.50 Kit contains n-Decyl−α−D-maltopyranoside, n-Dodecyl−α−D-maltopyranoside, and n-Undecyl−α−D-maltopyranoside . DSOL-ION IONIC DETERGENT KIT 41.00 Kit contains Sodium cholate, n-Dodecyl-N,N-dimethylglycine, n-Decyl-N,N- dimethylglyine, Sodium dodecanoyl sarcosine, and Deoxycholic acid, sodium salt. DSOL-M611 MALTOPYRANOSIDE DETERGENT KIT 62.00 Kit contains n-Hexyl−β−D-maltopyranoside, n-Octyl−β−D-maltopyranoside, n-Nonyl−β−D-maltopyranoside, n-Decyl−β−D-maltopyranoside and n-Undecyl−β−D-maltopyranoside. DSOL-M1214 MALTOPYRANOSIDE DETERGENT KIT 62.00 Kit contains n-Dodecyl−β−D-maltopyranoside, n-Tridecyl−β−D-maltopyranoside and n-Tetradecyl−β−D-maltopyranoside. INDEX DSOL-MS MISCELLANEOUS DETERGENT KIT 72.50 Kit contains CHAPS, FOS-CHOLINE®-ISO-9, CHAPSO, DDAO, CYPFOS™-3 and TDAO.

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DSOL-MSS MISCELLANEOUS SUGAR-BASED DETERGENT KIT 83.00 Kit contains 2,6 Dimethyl-4-heptyl−β−D-maltopyranoside, ANAMEG®-7, Sucrose monododecanoate, CYGLU®-3, Big CHAP, and Big CHAP, deoxy. DSOL-OX OXIDE DETERGENT KIT 51.50 Kit contains Dimethyldecylphosphine oxide, n-Tetradecyl-N,N-dimethylamine- N-oxide (TDAO) and n-Dodecyl-N,N-dimethylamine-N-oxide (DDAO). DSOL-PM PMAL™ DETERGENT KIT 104.00 Kit contains PMAL™-C8, PMAL™-C10, and PMAL™-C12. DSOL-CE POLYOXYETHYLENE DETERGENT KIT 62.00

Kit contains C8E4, C8E6, C10E5 and C12E8. DSOL-POP POPULAR DETERGENT KIT 72.50 Kit contains CHAPS, CYMAL®-5, n-Decyl−β−D-maltopyranoside, n-Dodecyl−β−D-maltopyranoside, FOS-CHOLINE®-12, and PRODUCT INFOMATION n-Octyl−β−D-glucopyranoside. DSOL-QUAT QUATERNARY DETERGENT KIT 30.50 Kit contains Decyltrimethylammonium chloride, Dodecyltrimethylammonium chloride, Hexadecyltrimethylammonium chloride, Octadecyltrimethylammonium chloride, and Tetradecyltrimethylammonium chloride. DSOL-TM THIOMALTOPYRANOSIDE DETERGENT KIT 72.50 Kit contains n-Octyl−β−D-Thiomaltopyranoside, n-Nonyl−β−D-Thiomaltopyranoside,

NEW PRODUCTS n-Decyl−β−D-Thiomaltopyranoside, n-Dodecyl−β−D-Thiomaltopyranoside and n-Undecyl−β−D-Thiomaltopyranoside. PRODUCT LISTING INDEX

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P23280 PeroXOquant™ Quantitative Peroxide Assay Kit 1 Kit 128.00 (Pierce Biotechnology, Inc.) Quickly measure peroxide contamination in various biological samples.

PeroXOquant™ Quantitative Peroxide Assays are the simplest The presence of hydrogen peroxide (H2O2) can now be

assays for detecting the presence of peroxides in both aqueous detected to monitor any peroxide contamination that may be PRODUCT INFORMATION and lipid-containing laboratory reagents. The basis of these harmful to biological samples. When performed on a routine 2+ assays is the complexing of ferric ion (Fe ) by H2O2 in the basis, the PeroXOquant™ Quantitative Peroxide Assay presence of xylenol orange. Peroxides in the sample oxidize can prevent inadvertent introduction of peroxides into your Fe2+ to Fe3+, and the Fe3+ will form a colored complex with valuable samples. If the effects of peroxide cannot be avoided xylenol orange that can be read at 560 nm. in a particular system, these assays will help you assess the risk to your sample. Features/Benefits: References: * Fast and easy to use 1. Jiang, Z.-Y., et al. (1991). Lipids 26(10), 853-856. * Peroxidase independent 2. Jiang, Z.-Y., et al. (1992). Anal. Biochem. 202(2), 384-389. * No lipid extraction necessary * Spectrophotometric analysis * No heating required NEW PRODUCTS

Comparison of Assay Protocols for Lipid Peroxide Content PeroXOquant™ Quantitative Peroxide Assay Thiobarbituric Acid Assay

1. Mix one volume of Reagent A with 100 volumes 1. Mix 0.1 ml sample, 0.4 ml H2O and 0.2 ml 7% SDS. of Reagent C to prepare Working Reagent. 2. Stir gently and add 2 ml 0.1 N HCI. 2. Add 950 μl of Working Reagent to 50 μl of sample. 3. Add 0.3 ml 10% phosphotungstic acid. 3. Incubate at room temperature for 30 minutes. 4. Incubate 5 minutes at room temperature. 4. Read at 560 nm (or 595 nm for ELISA plate readers). 5. Add 1 ml 0.67% thiobarbituric acid (TBA) and acetic acid. Total Time: 35 Minutes 6. Heat for 45 minutes at 95°C. 7. Cool in ice bath. 8. Add 5 ml butanol. 9. Vortex and centrifuge for 15 minutes. 10. Determine lipid peroxide concentration in butanol layer by fluorescence at 515 nm excitation and 553 nm emission. Total Time: 80-90 Minutes Ordering Information: Product # Description Pkg Size U.S. Price P23280 PeroXOquant™ Quantitative Kit $128.00 Peroxide Assay Kit Aqueous compatible formulation. Includes: Reagent A (25 mM Ammonium 1 ml Ferrous Sulfate) Reagent B (125 μM Xylenol 2x50 ml Orange in water with ) R:35; S:24/25/26,28 Available to our U.S.A. customers only. INDEX

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 111 Technical Data Detergent Solubility It is frustrating when one prepares a detergent solution that precipitates upon cooling to 4oC, often in a time dependent fashion over several days. There are several possible reasons for such precipitation. Combination of reagents causes precipitation Is the pH and ionic strength compatible with each of the components? The presence of an organic solvent as one component can result in the precipitation of some detergents. Try making a solution of each component to test its solubility in the relevant buffer. Microbial growth causes precipitation Many reagents used in protein research are readily biodegradable. For example, alkyl glyco- side detergents can easily be degraded by microorganisms, a process which can occur over a PRODUCT INFOMATION period of days and which results in the appearance of a precipitate or suspension. Microbial growth can conveniently be suppressed by including 1 mM EDTA in the buffer or by including a very low concentration of a bacteriocidal agent such as sodium azide. Solutions may also be sterilized by filtering through a 0.2 mm filter. Presence of synthetic precursors Many alkyl glycosides and alkyl maltosides will contain some of the parent alcohol, which may

NEW PRODUCTS not be completely removed during purification. When a concentrated solution of detergent is prepared and cooled to 4oC, a milkiness or cloudiness may become apparent due to precipita- tion or oil phase formation by the alcohol. This can be avoided by using highly purified detergents which are commercially available, such as ANAGRADE® detergents which contain less than 0.005% (w/w) of the parent alcohol, as compared with levels as high as 0.5% in some commercial preparations of the corresponding detergents. Kinetic effect of detergent solubilization Some detergents require heating for efficient solubilization. For example, when tetradecyl−β−

PRODUCT LISTING D-maltoside is dissolved at a concentration of 20% (w/v) at room temperature and cooled to 4°C, a precipitate appears. However, if the detergent solution is heated to 50°C before cool- ing, the precipitate does not form. Possibly the tetradecyl−β−D-maltoside forms optically clear colloids at room temperature which reprecipitate when cooled. Thus the detergent was never truly dissolved. This phenomenon has also been observed with HEGA®-10. Supersaturation A concentration of detergent which is fully soluble at room temperature may be supersatu- rated at 4oC. This is commonly observed for solutions containing sodium dodecyl sulfate. Supersaturated solutions can exist for days or even weeks before precipitation occurs. The only recourse when supersaturation is confirmed, is to reduce the detergent concentration or find an alternate detergent that is more soluble. For example, n-decyl−β−D- glucopyranoside is only slightly soluble in water (~0.1% w/v), but several closely related detergents are quite soluble: n-nonyl−β−D-glucopyranoside, n-decyl−β−D-maltopyranoside,

INDEX n-nonyl−β−D-thiomaltopyranoside, and n-undecyl−β−D-maltopyranoside. Each of these detergents are soluble to 20% (w/v) or greater in water.

112 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Technical Data SERVICE INFORMATION Detergent Grades Generally, detergent purity is not as critical for extraction as for protein studies and crystallization pro- cesses. When selecting a grade of detergent for extraction and purification, impurities that might degrade or modify proteins should be avoided. Polyoxyethylene detergents which may contain organic peroxides can modify the sulfhydryl groups of proteins. Most of the impurities in the sugar-based nonionic deter- gents are not reactive and therefore are unlikely to harm the membrane protein being extracted. The PRODUCT INFORMATION impurities of the maltopyranosides and glucopyranosides are generally the corresponding alcohol of the tail portion of the detergent or a detergent with a very similar structure. The presence of alcohol can lead to a cloudy solution, particularly when the protein solution is cool. Since the starting aliphatic material-- whether it be the corresponding alcohol, amine, or other functional group--will contain traces of other aliphatic compounds, the detergent will likewise contain traces of detergents with different chain lengths. During extraction, the presence of small amounts of closely related detergents should not present a prob- lem. For physiological studies and for crystallization of membrane proteins the purity of the detergents is much more important. Generally, the highest purity available should be utilized. In most instances, the detergent NEW PRODUCTS of choice is a nonionic detergent. The purity should be listed as 99% or greater as measured by a reliable test, such as HPLC. The conductance should be very low, indicating that there are few ionic impurities. For those nonionic detergents without ultraviolet chromophores, both the ultraviolet absorbance and the fluorescence background should be very low. Related detergents and aliphatic alcohols should be low so as not to be detectable by HPLC or other reliable tests. It is also considered important by some researchers that the detergent be isomerically homogenous. Thus, the aliphatic maltoside and glucoside detergents should be 99% alpha or beta isomer. The following discussion of grades of dodecyl maltoside, one of our most popular detergents, would apply to selecting an appropriate grade of any detergent in our catalog. Three grades of dodecyl maltoside which we offer are D310, D310A and D310S. The grade you purchase depends on your application. D310S and D310A are usually of sufficient purity for extraction and purifica- tion of membrane proteins. Indeed, D310S is greater than 98% pure and often is more pure than the dodecyl maltoside you may purchase from other suppliers. D310A is greater than 99% pure but contains up to 15% alpha isomer. D310 is 99% pure or greater and meets many other purity requirements, such as low absorbance in the UV. This grade is generally used when purification steps require high purity deter- gent or for studies using a purified membrane protein. An additional grade (D310LA) contains less than 0.2% alpha isomer. Some researchers find this grade preferable for crystallization studies. The alpha isomer itself is also available (D310HA). For specifications on grades D310LA and D310HA please see pages 52 and 53. If you don't see the particular grade or detergent you need in our catalog, let us know and we can probably prepare it for you.

Please note: The terms maltopyranoside and maltoside are used interchangeably for the same class of

compounds. Likewise, glucopyranoside and glucoside refer to the same structure. Alkyl glycoside is a INDEX more general term used for any simple sugar derivative. Thus, all glucosides are glycosides, but not all glycosides are glucose derivatives or glucosides.

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 113 Technical Data Detergent Stability

The stability of a representative sample of detergents has been tested both in solution and in solid form at Anatrace, Inc. The purpose of this study was to determine guidelines for shipping and storage of solid detergents and to give some indication of their stability in solution.

To determine their stability during shipment, these representative detergents: Dodecyl−β−D-maltoside, CYMAL®-3, HEGA®-10, MEGA-9, Octyl−β−D-glucoside, CHAPS, CHAPSO, Dodecyl-N,N-dimethylglycine, Octyl−β−D-galactoside, Sodium cholate, Dodecyl-N,N-dimethylamine oxide, and FOS-CHOLINE®-10, were stored at 40°C for one week and room temperature for two weeks. No deterioration was PRODUCT INFOMATION noted after this time when the specifications for each sample were remeasured. That is, all detergent samples remained within specifications. See pages 52, 59 and 82 for specifications for Dodecyl−β−D-maltoside, FOS-CHOLINE®-10 and Octyl−β− D-glucoside.

To obtain an indication of the stability of detergents in solution, ten percent

NEW PRODUCTS aqueous solutions of tetradecyl maltoside, dodecyl maltoside and HEGA®-10 were heated to 55°C for two hours. Measurement of the percent purity by HPLC, pH, conductance and absorbance indicated these detergents had not deteriorated during this heat cycle.

It is our goal to supply only the best quality detergents and other reagents. Hence, we will continue to ship detergents to you by an overnight service when possible. PRODUCT LISTING INDEX

114 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Technical Data SERVICE INFORMATION Detergent Properties Listed Alphabetically The detergents are arranged in alphabetical order. The conditions used to measure CMC values and aggregation numbers are located in the Alphabetial Product Listing section of the catalog.

Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # A340 20 ANAMEG®-7 N 335.4 19.5 (0.65%) 92

APT020 21 ANAPOE®-20 N 1228 0.059 (0.0072%) PRODUCT INFORMATION APB035 21 ANAPOE®-35 N 1198 0.091 (0.0010%) 40 APB058 21 ANAPOE®-58 N 1122 0.004 (0.00045%) APT080 22 ANAPOE®-80 N 1310 0.012 (0.0016%) 58 ® APO106 22 ANAPOE -C10E6 N 423 0.9 (0.0250%) 40 ® APO109 22 ANAPOE -C10E9 N 555 1.3 (0.053%) ® APO128 23 ANAPOE -C12E8 N 539 0.09 (0.0048%) 123 ® APO129 23 ANAPOE -C12E9 N 583 0.05 (0.003%) ® AP1210 23 ANAPOE -C12E10 N 627 0.2 ® APO138 23 ANAPOE -C13E8 N 553 0.1 (0.0055%) APND40 24 ANAPOE®-NID-P40 N 603 0.05-0.3 100-155 APX100 24 ANAPOE®-X-100 N 647 0.23 (0.015%) 75-165 NEW PRODUCTS APX114 24 ANAPOE®-X-114 N 536 0.2 (0.011%) APX305 25 ANAPOE®-X-305 N 1526 APX405 25 ANAPOE®-X-405 N 1967 0.81 (0.16%) AZ308 25 ANZEGENT™ 3-8 Z 279.6 390 (10.9%) AZ310 26 ANZERGENT® 3-10 Z 307.6 39 (1.2%) 41 AZ312 26 ANZERGENT® 3-12 Z 335.5 2.8 (0.094%) 55-87 AZ314 27 ANZERGENT® 3-14 Z 363.6 0.2 (0.007%) 83-130 AZ316 27 ANZERGENT® 3-16 Z 391.65 10-60 AZ318 28 ANZERGENT® 3-18 Z 417.71 B300 28 Big CHAP N 878.1 2.9 (0.25%) 10 B310 28 Big CHAP, deoxy N 862.1 1.4 (0.12%) 8, 16 C640 29 C-DODECAFOS™ Z 349.17 22 (0.77%) C630 29 C-OCTAFOS™ Z 293.13 450 (13%) C316 30 CHAPS Z 614.9 8 (0.49%) 10 C317 31 CHAPSO Z 630.9 8 (0.50%) 11 C408 31 C-HEGA®-8 N 349.5 277 (9.7%) C409 32 C-HEGA®-9 N 363.5 108 (3.9%) C410 32 C-HEGA®-10 N 377.5 35 (1.3%) C411 32 C-HEGA®-11 N 391.5 11.5 (0.45%) C508 34 CYCLOFOS™-2 Z 293.8 256 (7.5%) C510 34 CYCLOFOS™-3 Z 306.91 43 (1.3%) C512 34 CYCLOFOS™-4 Z 320.92 14 (0.45%) C514 35 CYCLOFOS™-5 Z 334.95 4.5 (0.15%) C516 35 CYCLOFOS™-6 Z 349.17 2.68 (0.094%) C518 35 CYCLOFOS™-7 N 363.3 0.62 (0.022%) C702 36 CYGENT™ 1-3 Z 269.4 500 C323G 36 CYGLU®-3 N 304.4 28 (0.86%) C324G 37 CYGLU®-4 N 318.4 1.8 (0.058%) C321 37 CYMAL®-1 N 438.5 340 (15%) 360 C322 38 CYMAL®-2 N 452.5 120 (5.4%) 104 C324 39 CYMAL®-4 N 480.5 7.6 (0.37%) 25 C325 39 CYMAL®-5 N 494.5 2.4 (0.12%) 47 C326 40 CYMAL®-6 N 508.5 0.56 (0.028%) 91 INDEX C327 42 CYMAL®-7 N 522.5 0.19 (0.0099%) 150 C610 43 CYPFOS™-3 Z 293.3 180 (5.3%) D365 43 n-Decyl-N,N-dimethylamine-N-oxide Z 201.36 10.48 (0.211%)

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Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # D352 43 n-Decyl-N,N-dimethylglycine Z 243.4 19 (0.46%) D321 44 n-Decyl−β−D-glucopyranoside N 320.4 2.2 (0.070%) D322 44 n-Decyl−β−D-maltopyranoside N 482.6 1.8 (0.087%) 69 D322HA 45 n-Decyl-α-D-maltopyranoside N 482.6 N/A D322LA 45 n-Decyl−β−D-maltopyranoside N 482.6 1.8 (0.087%) 69 D323 46 n-Decyl−β−D-thioglucopyranoside N 336.4 0.9 (0.30%) D335 47 n-Decyl−β−D-thiomaltopyranoside N 498.6 0.9 (0.045%) 75 D354 47 Decyltrimethylammonium chloride C 236.0 2.95 (0.07%) D380 47 Deoxycholic acid A 414.6 6 (0.24%) 22 D412 48 DIFOS™-12 Z 532.6 1.18 (0.063%) DH325 48 2,6-Dimethyl-4-heptyl−β−D-maltoside N 468.5 27.5 (1.2%) D330 48 Dimethyldecylphosphine oxide N 218.3 4.66 (0.10%) 131 D328 49 Dimethyloctylphosphine oxide N 190.1 40 (0.76%) 41 D360 49 n-Dodecyl-N,N-dimethylamine-N-oxide Z 229.41 1 (0.023%) 76 D350 50 n-Dodecyl-N,N-dimethylglycine Z 271.4 1.5 (0.041%) D318 51 n-Dodecyl−β−D-glucopyranoside N 348.5 0.19 (0.0066%)

PRODUCT INFOMATION D345 51 n-Dodecyl−β−iminodipropionic acid, A 373.4 N/A disodium salt D346 51 n-Dodecyl−β−iminodipropionic acid, Z 351.5 N/A monosodium salt D310HA 52 n-Dodecyl-α-D-maltopyranoside N 510.6 0.15 (0.0076%) 90 D310 52 n-Dodecyl−β−D-maltopyranoside N 510.6 0.17 (0.0087%) 78-149 D342 55 n-Dodecyl−β−D-thiomaltopyranoside N 526.6 0.05 (0.0026%) 126 D355 55 Dodecyltrimethylammonium Chloride C 263.89 0.046 (0.0012%) 44-61 F300 58 FOS-CHOLINE®-8 Z 295.4 114 (3.4%) NEW PRODUCTS F302 58 FOS-CHOLINE®-9 Z 309.4 39.5 (1.2%) F304 59 FOS-CHOLINE®-10 Z 323.4 11 (0.35%) F306 60 FOS-CHOLINE®-11 Z 337.4 1.85 (0.062%) 18 F308 60 FOS-CHOLINE®-12 Z 351.5 1.5 (0.047%) 54 F310 61 FOS-CHOLINE®-13 Z 365.5 0.75 (0.027%) 87 F312 62 FOS-CHOLINE®-14 Z 379.5 0.12 (0.0046%) 108 F312D 62 FOS-CHOLINE®-14 Z 421.5 0.12 (0.0051%) F314 63 FOS-CHOLINE®-15 Z 393.5 0.07 (0.0027%) 131 F316 64 FOS-CHOLINE®-16 Z 407.5 0.013 (0.00053%) 178 FCI09 64 FOS-CHOLINE®-ISO-9 Z 309 32 (0.99%)

PRODUCT LISTING FCI11 65 FOS-CHOLINE®-ISO-11 Z 337.4 26.6 (0.9%) FCI116 65 FOS-CHOLINE®-ISO-11-6U Z 337.16 25.8 (0.87%) FCU110 65 FOS-CHOLINE®-UNSAT-11-10 Z 335.4 6.2 (0.21%) F208 66 FOS-MEA®-8 A 267.01 22 (0.59%) F210 66 FOS-MEA®-10 A 295.01 5.25 (0.15%) F212 66 FOS-MEA®-12 A 323.01 0.43 (0.014%) FF360 67 FOSFEN™-9 Z 385.21 1.35 (0.052%) H108 67 HEGA®-8 N 351.5 109 (3.8%) H109 68 HEGA®-9 N 365.5 39 (1.4%) H110 68 HEGA®-10 N 379.5 7 (0.26%) H111 68 HEGA®-11 N 393.5 1.4 (0.055%) H300 69 n-Heptyl−β−D-glucopyranoside N 278.4 70 (1.9%) H300LA 69 n-Heptyl−β−D-glucopyranoside N 278.4 70 (1.9%) H301 70 n-Heptyl−β−D-thioglucopyranoside N 294.4 29 (0.85%) H301LA 70 n-Heptyl−β−D-thioglucopyranoside N 294.4 29 (0.85%) H320 70 n-Hexadecyl−β−D-maltopyranoside N 566.6 0.0006 (0.00003%) H355 71 Hexadecyltrimethylammonium chloride C 320.0 0.0032 (0.000102%) 61-84 H360 71 Hexaethylene glycol monodecyl ether N 422.6 0.9 73 INDEX H350 71 Hexaethylene glycol monooctyl ether N 394.5 10 (0.39%) 32 H310 72 n-Hexyl−β−D-maltopyranoside N 426.4 210 (8.9%) L360S 74 LAPAO Z 300.55 1.56 (0.052%) 126

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Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # M319 75 MEGA-8 N 321.4 79 (2.5%) M325 75 MEGA-9 N 335.5 25 (0.84%) M320 76 MEGA-10 N 349.5 6 (0.21%) NIDP40 77 Nonidet P40 Substitute N 603 50 (0.05-0.3%) 100-155 N324 78 n-Nonyl−β−D-glucopyranoside N 306.4 6.5 (0.20%) N324LA 78 n-Nonyl−β−D-glucopyranoside N 306.4 6.5 (0.20%) N330 79 n-Nonyl−β−D-maltopyranoside N 468.5 6 (0.28%) 55 PRODUCT INFORMATION N335 80 n-Nonyl−β−D-thioglucopyranoside N 322.4 2.9 (0.093%) N350 80 n-Nonyl−β−D-thiomaltopyranoside N 484.6 3.2 (0.15%) N150 80 NOPOL-FOS™ Z 331.4 42.5 (1.4%) O355 81 Octadecyltrimethylammonium chloride C 348.1 < 0.03 (0.001%) O330 81 Octaethylene glycol monododecyl ether N 538.77 0.09 (0.0048%) 90-120 O312 82 n-Octyl−β−D-galactopyranoside N 292.4 29.5 (0.86%) O311 82 n-Octyl−β−D-glucopyranoside N 292.4 18 (0.53%) 78 O311HA 83 n-Octyl-α-D-glucopyranoside N 292.4 .010 (0.00029%) O310 85 n-Octyl−β−D-maltopyranoside N 454.4 19.5 (0.89%) 47 O314 86 n-Octyl−β−D-thioglucopyranoside N 308.4 9 (0.28%) O314LA 86 n-Octyl−β−D-thioglucopyranoside N 308.4 9 (0.28%)

O320 86 n-Octyl−β−D-thiomaltopyranoside N 470.6 8.5 (0.40%) NEW PRODUCTS P340 87 Pentaethylene glycol monodecyl ether N 378.55 0.81 (0.031%) 73 P350 87 Pentaethylene glycol monooctyl ether N 350.5 7.1 (0.25%) P5008 88 PMAL™-C8 Z N/A P5010 88 PMAL™-C10 Z N/A P5012 88 PMAL™-C12 Z N/A P5016 89 PMAL™-C16 Z N/A P310 90 2-Propyl-1-pentyl maltopyranoside N 455.5 42.5 (1.9%) S1010 91 Sodium cholate A 430.6 9.5 (0.41%) 2-3 S300 91 Sodium dodecanoyl sarcosine A 293.4 14.4 (0.42%) S1001 92 Sodium dodecyl sulfate A 288.38 2.6 (0.075%) 62-101 S2033 93 Sodium taurocholate A 537.7 3-11 (0.16-0.59%) 4 S350 93 Sucrose monododecanoate N 524.61 0.3 (0.016%) T360 94 n-Tetradecyl-N,N-dimethylamine-N-oxide Z 257.46 0.29 (0.0075%) T305 94 n-Tetradecyl-N,N-dimethylglycine Z 299.4 0.034 (0.0010%) T315 94 n-Tetradecyl−β−D-maltopyranoside N 538.6 0.01 (0.00054%) T355 95 Tetradecylmethylammonium chloride C 279.9 0.032 (0.0009%) 62-81 T350 95 Tetraethylene glycol monooctyl ether N 306.45 8 (0.25%) 82 T323 96 n-Tridecyl−β−D-maltopyranoside N 524.6 0.033 (0.0017%) 186 T370 97 TRIPAO Z 362.55 4.5 T1001 98 Triton® X-100 N 647 0.23 (0.015%) 75-165 T1002 98 Triton® X-114 N 536 0.2 (0.011%) T1003 98 Tween® 20 N 1228 0.059 (0.0072%) T1004 99 Tween® 80 N 1310 0.012 (0.0016%) 58 U300 100 n-Undecyl−β−D-maltopyranoside N 496.6 0.59 (0.029%) 71 U300HA 100 n-Undecyl-α-D-maltopyranoside N 496.6 0.58 (0.029%) U342 102 n-Undecyl−β−D-thiomaltopyranoside N 512.7 0.21 (0.011%) 106 U360 99 n-Undecyl-N,N-dimethylamine-oxide Z 215.38 3.21 (0.069%) U310 102 ω-Undecylenyl−β−D-maltopyranoside N 494.6 1.2 (0.059%) INDEX

The types of detergents: A = Anionic / C = Cationic / N = Nonionic / Z = Zwitterionic

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 117 Technical Data Detergent Properties Listed by CMC Values The detergents are arranged in the order of their CMC values. The conditions used to measure CMC values and aggregation numbers are located in the Alphabetial Product Listing section of the catalog.

Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # APX305 25 ANAPOE®-X-305 N 1526 N/A D322HA 45 n-Decyl−α−D-maltopyranoside N 482.6 N/A D345 51 Dodecyl−β−iminodipropionic acid, A 373.4 N/A disodium salt D346 51 Dodecyl−β−iminodipropionic acid, Z 351.5 N/A monosodium salt P5008 88 PMAL™-C8 Z N/A P5010 88 PMAL™-C10 Z N/A P5012 88 PMAL™-C12 Z N/A P5016 89 PMAL™-C16 Z N/A

PRODUCT INFOMATION H320 70 n-Hexadecyl−β−D-maltopyranoside N 566.6 0.0006 (0.00003%) H355 71 Hexadecyltrimethylammonium chloride C 320.0 0.0032 (0.000102%) 61-84 APB058 21 ANAPOE®-58 N 1122 0.004 (0.00045%) O311HA 83 n-Octyl−α−D-glucopyranoside N 292.4 .010 (0.00029%) T315 94 n-Tetradecyl−β−D-maltopyranoside N 538.6 0.01 (0.00054%) APT080 22 ANAPOE®-80 N 1310 0.012 (0.0016%) 58 T1004 99 TWEEN® 80 N 1310 0.012 (0.0016%) 58 F316 64 FOS-CHOLINE®-16 Z 407.5 0.013 (0.00053%) 178 T1001 98 TRITON® X-100 N 647 0.010-0.016 (0.015%) 75-165

NEW PRODUCTS O355 81 Octadecyltrimethylammonium chloride C 348.1 < 0.03 (0.001%) T355 95 Tetradecylmethylammonium chloride C 279 .9 0.032 (0.0009%) 62-81 T323 96 n-Tridecyl−β−D-maltopyranoside N 524.6 0.033 (0.0017%) 186 T323LA 96 n-Tridecyl−β−D-maltopyranoside N 524.6 0.033 (0.0017%) 186 T305 94 n-Tetradecyl-N,N-dimethylglycine Z 299.4 0.034 (0.0010%) D355 55 Dodecyltrimethylammonium Chloride C 263.89 0.046 (0.0012%) 44-61 ® APO129 23 ANAPOE -C12E9 N 583 0.05 (0.003%) D342 55 n-Dodecyl−β−D-thiomaltopyranoside N 526.6 0.05 (0.0026%) 126 APND40 24 ANAPOE®-NID-P40 N 603 0.05-0.3 100-155 APT020 21 ANAPOE®-20 N 1228 0.059 (0.0072%) ®

PRODUCT LISTING T1003 98 TWEEN 20 N 1228 0.059 (0.0072%) F314 63 FOS-CHOLINE®-15 Z 393.5 0.07 (0.0027%) 131 ® APO128 23 ANAPOE -C12E8 N 538.77 0.09 (0.0048%) 123 O330 81 Octaethylene glycol monododecyl ether N 538.77 0.09 (0.0048%) 90 APB035 21 ANAPOE®-35 N 1198 0.091 (00110%) 40 ® APO138 23 ANAPOE -C13E8 N 553 0.1 (0.0055%) F312 62 FOS-CHOLINE®-14 Z 379.5 0.12 (0.0046%) 108 F312D 62 FOS-CHOLINE®-14, deuterated Z 421.5 0.12 (0.0051%) D310HA 52 n-Dodecyl−α−D-maltopyranoside N 510.6 0.15 (0.0076%) 90 D310 52 n-Dodecyl−β−D-maltopyranoside N 510.6 0.17 (0.0087%) 78-149 C327 42 CYMAL®-7 N 522.5 0.19 (0.0099%) 150 D318 51 n-Dodecyl−β−D-glucopyranoside N 348.5 0.19 (0.0066%) ® AP1210 23 ANAPOE -C12E10 N 627 0.2 AZ314 27 ANZERGENT® 3-14 Z 363.6 0.2 (0.007%) 83-130 APX114 24 ANAPOE®-X-114 N 536 0.2 (0.011%) T1002 98 TRITON® X-114 N 536 0.2 (0.011%) U342 102 n-Undecyl−β−D-thiomaltopyranoside N 512.7 0.21 (0.011%) 106 APX100 24 ANAPOE®-X-100 N 647 0.23 (0.015%) 75-165

INDEX T360 94 n-Tetradecyl-N,N-dimethylamine-N-oxide Z 257.46 0.29 (0.0075%) S350 93 Sucrose monododecanoate N 524.61 0.3 (0.016%) F212 6 6 FOS-MEA®-12 A 323.01 0.43 (0.014%)

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Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # C326 40 CYMAL®-6 N 508.5 0.56 (0.028%) 91 C326LA 41 CYMAL®-6 N 508.5 0.56 (0.028%) 91 U300HA 100 n-Undecyl−α−D-maltopyranoside N 496.6 0.58 (0.029%) U300 100 n-Undecyl−β−D-maltopyranoside N 496.6 0.59 (0.029%) 71 C518 35 CYCLOFOS™-7 N 363.3 0.62 (0.022%) F310 61 FOS-CHOLINE®-13 Z 365.5 0.75 (0.027%) 87 P340 87 Pentaethylene glycol monodecyl ether N 378.55 0.81 (0.031%) 73

APX405 25 ANAPOE®-X-405 N 1967 0.81 (0.16%) PRODUCT INFORMATION ® APO106 22 ANAPOE -C10E6 N 423 0.9 (0.025%) 40 D323 46 n-Decyl−β−D-thioglucopyranoside N 336.4 0.9 (0.30%) D335 47 n-Decyl−β−D-thiomaltopyranoside N 498.6 0.9 (0.045%) 75 D360 49 n-Dodecyl-N,N-dimethylamine-N-oxide Z 229.41 1 (0.023%) 76 D412 48 DIFOS™-12 Z 532.6 1.18 (0.063%) U310 102 ω-Undeceylenyl−β−D-maltopyranoside N 494.6 1.2 (0.059%) ® APO109 22 ANAPOE -C10E9 N 555 1.3 (0.053%) FF360 67 FOSFEN™-9 Z 385.21 1.35 (0.052%) B310 28 Big CHAP, deoxy N 862.1 1.4 (0.12%) 8, 16 H111 68 HEGA®-11 N 393.5 1.4 (0.055%) D350 50 n-Dodecyl-N,N-dimethylglycine Z 271.4 1.5 (0.041%) NEW PRODUCTS F308 60 FOS-CHOLINE®-12 Z 351.5 1.5 (0.047%) 54 L360S 74 LAPAO Z 300.55 1.56 (0.052%) 126 C324G 37 CYGLU®-4 N 318.4 1.8 (0.058%) D322 44 n-Decyl−β−D-maltopyranoside N 482.6 1.8 (0.087%) 69 D322LA 45 n-Decyl−β−D-maltopyranoside N 482.6 1.8 (0.087%) 69 F306 60 FOS-CHOLINE®-11 Z 337.4 1.85 (0.062%) 18 D321 44 n-Decyl−β−D-glucopyranoside N 320.4 2.2 (0.070%) C325 39 CYMAL®-5 N 494.5 2.4 (0.12%) 47 S1001 92 Sodium dodecyl sulfate A 288.38 2.6 (0.075%) 62-101 C516 35 CYCLOFOS™-6 Z 349.17 2.68 (0.094%) AZ312 26 ANZERGENT® 3-12 Z 335.5 2.8 (0.094%) 55-87 B300 28 Big CHAP N 878.1 2.9 (0.25%) 10 N335 80 n-Nonyl−β−D-thioglucopyranoside N 322.4 2.9 (0.093%) D354 47 Decyltrimethylammonium chloride C 236.0 2.95 (0.07%) S2033 93 Sodium taurocholate A 537.7 3-11 (0.16-0.59%) 4 N350 80 n-Nonyl−β−D-thiomaltopyranoside N 484.6 3.2 (0.15%) U360 99 n-Undecyl-N,N-dimethylamine-oxide Z 215.38 3.21 (0.069%) C514 35 CYCLOFOS™-5 Z 334.95 4.5 (0.15%) D330 48 Dimethyldecylphosphine oxide N 218.3 4.66 (0.10%) 131 F210 6 6 FOS-MEA®-10 A 295.01 5.25 (0.15%) D380 47 Deoxycholic acid A 414.6 6 (0.24%) 22 M320 76 MEGA-10 N 349.5 6 (0.21%) N330 79 n-Nonyl−β−D-maltopyranoside N 468.5 6 (0.28%) 55 FCU110 65 FOS-CHOLINE®-UNSAT-11-10 Z 335.4 6.2 (0.21%) N324 78 n-Nonyl−β−D-glucopyranoside N 306.4 6.5 (0.20%) N324LA 78 n-Nonyl−β−D-glucopyranoside N 306.4 6.5 (0.20%) H110 68 HEGA®-10 N 379.5 7 (0.26%) P350 87 Pentaethylene glycol monooctyl ether N 350.5 7.1 (0.25%) C324 39 CYMAL®-4 N 480.5 7.6 (0.37%) 25 C316 30 CHAPS Z 614.9 8 (0.49%) 10 C317 31 CHAPSO Z 630.9 8 (0.50%) 11 T350 95 Tetraethylene glycol monooctyl ether N 306.45 8 (0.25%) 82 O320 86 n-Octyl−β−D-thiomaltopyranoside N 470.6 8.5 (0.40%) INDEX O314 86 n-Octyl−β−D-thioglucopyranoside N 308.4 9 (0.28%) O314LA 86 n-Octyl−β−D-thioglucopyranoside N 308.4 9 (0.28%) S1010 91 Sodium cholate A 430.6 9.5 (0.41%) 2 H350 71 Hexaethylene glycol monooctyl ether N 394.5 10 (0.39%) 32

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Catalog # Page # Detergent Type FW CMC mM (%) Aggregation # D365 43 n-Decyl-N,N-dimethylamine-N-oxide Z 201.36 10.48 (0.211%) F304 59 FOS-CHOLINE®-10 Z 323.4 11 (0.35%) C411 32 C-HEGA®-11 N 391.5 11.5 (0.45%) C512 34 CYCLOFOS™-4 Z 320.92 14 (0.45%) S300 91 Sodium dodecanoyl sarcosine A 293.4 14.4 (0.42%) O311 82 n-Octyl−β−D-glucopyranoside N 292.4 18 (0.53%) 78 D352 43 n-Decyl-N,N-dimethylglycine Z 243.4 19 (0.46%) A340 20 ANAMEG®-7 N 335.4 19.5 (0.65%) 92 O310 85 n-Octyl−β−D-maltopyranoside N 454.4 19.5 (0.89%) 47 C640 29 C-DODECAFOS™ Z 349.17 22.0 (0.77%) F208 6 6 FOS-MEA®-8 A 267.01 22 (0.59%) M325 75 MEGA-9 N 335.5 25 (0.84%) FCI116 65 FOS-CHOLINE®-ISO-11-6U Z 337.16 25.8 (0.87%) FCI11 65 FOS-CHOLINE®-ISO-11 Z 337.4 26.6 (0.9%) DH325 48 2,6-Dimethyl-4-heptyl−β−D-maltoside N 468.5 27.5 (1.2%) C323G 36 CYGLU®-3 N 304.4 28 (0.86%)

PRODUCT INFOMATION H301 70 n-Heptyl−β−D-thioglucopyranoside N 294.4 29 (0.85%) H301LA 70 n-Heptyl−β−D-thioglucopyranoside N 294.4 29 (0.85%) O312 82 n-Octyl−β−D-galactopyranoside N 292.4 29.5 (0.86%) FCI09 64 FOS-CHOLINE®-ISO-9 Z 309 32 (0.99%) C323 38 CYMAL®-3 N 466.5 34.5 (1.6%) 38 C410 32 C-HEGA®-10 N 377.5 35 (1.3%) AZ310 26 ANZERGENT® 3-10 Z 307.6 39 (1.2%) 41 H109 68 HEGA®-9 N 365.5 39 (1.4%) F302 58 FOS-CHOLINE®-9 Z 309.4 39.5 (1.2%)

NEW PRODUCTS D328 49 Dimethyloctylphosphine oxide N 190.1 40 (0.76% ) 41 N150 80 NOPOL-FOS™ Z 331.4 42.5 (1.4%) P310 90 2-Propyl-1-pentyl-maltopyranoside N 455.5 42.5 (1.9%) C510 34 CYCLOFOS™-3 Z 306.91 43 (1.3%) NIDP40 77 Nonidet P40 Substitute N 603 50 (0.05-0.3%) 100-155 H300 69 n-Heptyl−β−D-glucopyranoside N 278.4 70 (1.9%) H300LA 69 n-Heptyl−β−D-glucopyranoside N 278.4 70 (1.9%) M319 75 MEGA-8 N 321.4 79 (2.5%) C409 32 C-HEGA®-9 N 363.5 108 (3.9%) H108 67 HEGA®-8 N 351.5 109 (3.8%) ®

PRODUCT LISTING F300 58 FOS-CHOLINE -8 Z 295.4 114 (3.4%) C322 38 CYMAL®-2 N 452.5 120 (5.4%) 104 C610 43 CYPFOS™-3 Z 293.3 180 (5.3%) H310 72 n-Hexyl−β−D-maltopyranoside N 426.4 210 (8.9%) H305 72 n-Hexyl−β−D-glucopyranoside N 264.4 250 (6.6%) C508 34 CYCLOFOS™-2 Z 293.8 256 (7.5%) C408 31 C-HEGA®-8 N 349.5 277 (9.7%) C321 37 CYMAL®-1 N 438.5 340 (15%) 360 AZ308 25 ANZERGENT® 3-8 Z 279.6 390 (10.9%) C630 29 C-OCTAFOS™ Z 293.13 450 (13%) C702 36 CYGENT™ 1-3 Z 269.4 500 INDEX

120 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION Catalog Number Index Cat. # Product Name Page # Cat. # Product Name Page # A110MT 2-Aminoethyl methanethiosulfonate hydrobromide 20 C630 C-OCTAFOS™, ANAGRADE® 29 A340 ANAMEG®-7, ANAGRADE® 20 C640 C-DODECAFOS™, ANAGRADE® 29 ™ ® A1000 Ampicillin, sodium salt 20 C702 CYGENT 1-3, ANAGRADE 36 ® C3000 CMC dye solution 33 AP1210 ANAPOE -C12E10 23 APB035 ANAPOE®-35 21 C6500 Culture tubes 33 APB058 ANAPOE®-58 21 CA100 Carbenicillin disodium salt 30 APND40 ANAPOE®-NID-P40 24 CH200 Cholesterol 33 PRODUCT INFORMATION ® CH210 Cholesteryl hemisuccinate tris salt 33 APO106 ANAPOE -C10E6 22 ® D310 n-Dodecyl−β−D-maltopyranoside, ANAGRADE® 52 APO109 ANAPOE -C10E9 22 ® D310A n-Dodecyl−β−D-maltopyranoside, ANAGRADE® 53 APO128 ANAPOE -C12E8 23 APO129 ANAPOE®-C E 23 D310HA n-Dodecyl−α−D-maltopyranoside, ANAGRADE® 52 12 9 ® APO138 ANAPOE®-C E 23 D310LA n-Dodecyl−β−D-maltopyranoside, ANAGRADE 53 13 8 ® APT020 ANAPOE®-20 21 D310S n-Dodecyl−β−D-maltopyranoside, SOL-GRADE 54 ® APT080 ANAPOE -80 22 D310T n-Dodecyl-d25−β−D-maltopyranoside 54 ® APX100 ANAPOE®-X-100 24 D318 n-Dodecyl−β−D-glucopyranoside, ANAGRADE 51 ® APX114 ANAPOE®-X-114 24 D321 n-Decyl−β−D-glucopyranoside, ANAGRADE 44 ® APX305 ANAPOE®-X-305 25 D322 n-Decyl−β−D-maltopyranoside, ANAGRADE 44 ® APX405 ANAPOE®-X-405 25 D322HA n-Decyl−α−D-maltopyranoside, ANAGRADE 45 ® ® D322LA n-Decyl−β−D-maltopyranoside, ANAGRADE 45 AZ308 ANZERGENT 3-8, Analytical Grade 25 NEW PRODUCTS D322S n-Decyl−β−D-maltopyranoside, SOL-GRADE® 46 ® AZ310 ANZERGENT 3-10, Analytical Grade 26 D323 n-Decyl−β−D-thioglucopyranoside, ANAGRADE® 46 AZ312 ANZERGENT® 3-12, Analytical Grade 26 D328 Dimethyloctylphosphine oxide 49 AZ314 ANZERGENT® 3-14, Analytical Grade 27 D330 Dimethyldecylphosphine oxide, ANAGRADE® 48 ® AZ316 ANZERGENT® 3-16, Analytical Grade 27 D335 n-Decyl−β−D-thiomaltopyranoside, ANAGRADE 47 D342 n-Dodecyl−β−D-thiomaltopyranoside, ANAGRADE® 55 AZ318 ANZERGENT® 3-18, Analytical Grade 28 D345 n-Dodecyl−β−iminodipropionic acid, disodium salt 51 B300 Big CHAP, Analytical Grade 28 D346 n-Dodecyl−β−iminodipropionic acid, monosodium salt 51 B310 Big CHAP, Deoxy, Analytical Grade 28 D350 n-Dodecyl-N,N-dimethylglycine, ANAGRADE® 50 ® B2000 X-GAL, ANAGRADE 103 D350S n-Dodecyl-N,N-dimethylglycine, SOL-GRADE® 50 B2010 Magenta-GAL 75 D352 n-Decyl-N,N-dimethylglycine, ANAGRADE® 43 B2020 X-Gluc, CHA Salt 104 D354 Decyltrimethylammonium chloride 47 B2030 X-Gluc, Na Salt 104 D355 Dodecyltrimethylammonium chloride 55 B2040 Blue-GAL 29 D360 n-Dodecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 49 B2050 XGAL-IPTG 103 D360S n-Dodecyl-N,N-dimethylamine-N-oxide,SOL-GRADE® 50 ® C316 CHAPS, ANAGRADE 30 D365 n-Decyl-N,N-dimethylamine-N-oxide, ANAGRADE® 43 ® C316S CHAPS, SOL-GRADE 31 D380 Deoxycholic acid, sodium salt, ANAGRADE® 47 ® C317 CHAPSO, ANAGRADE 31 D412 DIFOS™-12, ANAGRADE® 48 ® ® C321 CYMAL -1, ANAGRADE 37 D1000 DTNB 56 ® ® C322 CYMAL -2, ANAGRADE 38 D2000 DTT, Cleland's Reagent 56 ® ® C323 CYMAL -3, ANAGRADE 38 D2000A DTT, Cleland's Reagent, ANAGRADE® 56 ® ® C323G CYGLU -3, ANAGRADE 36 D2001 DTE 55 ® ® C324 CYMAL -4, ANAGRADE 39 DH325 2,6-Dimethyl-4-heptyl−β−D-maltopyranoside, ANAGRADE® 48 ® ® C324G CYGLU -4, ANAGRADE 37 E1000 EDTA 57 ® ® C325 CYMAL -5, ANAGRADE 39 E1001 EGTA 57 ® ® C325S CYMAL -5, SOL-GRADE 40 E2000 NEM 57 ® ® C326 CYMAL -6, ANAGRADE 40 F208 FOS-MEA®-8, ANAGRADE® 66 ® ® C326LA CYMAL -6, ANAGRADE 41 F210 FOS-MEA®-10, ANAGRADE® 66 ® ® C326S CYMAL -6, SOL-GRADE 41 F212 FOS-MEA®-12, ANAGRADE® 66 ® ® C327 CYMAL -7, ANAGRADE 42 F300 FOS-CHOLINE®-8, ANAGRADE® 58 ® ® C327S CYMAL -7, SOL-GRADE 42 F300S FOS-CHOLINE®-8, SOL-GRADE® 58 ® ® C408 C-HEGA -8, ANAGRADE 31 F302 FOS-CHOLINE®-9, ANAGRADE® 58 ® ® C409 C-HEGA -9, ANAGRADE 32 F302S FOS-CHOLINE®-9, SOL-GRADE® 59 ® ® C410 C-HEGA -10, ANAGRADE 32 F304 FOS-CHOLINE®-10, ANAGRADE® 59 ® ® C411 C-HEGA -11, ANAGRADE 32 F304S FOS-CHOLINE®-10, SOL-GRADE® 59 C508 CYCLOFOS™-2, ANAGRADE® 34 INDEX F306 FOS-CHOLINE®-11, ANAGRADE® 60 C510 CYCLOFOS™-3, ANAGRADE® 34 F306S FOS-CHOLINE®-11, SOL-GRADE® 60 C512 CYCLOFOS™-4, ANAGRADE® 34 F308 FOS-CHOLINE®-12, ANAGRADE® 60 C514 CYCLOFOS™-5, ANAGRADE® 35 F308D FOS-CHOLINE®-12, Deuterated 61 C516 CYCLOFOS™-6, ANAGRADE® 35 F308S FOS-CHOLINE®-12, SOL-GRADE® 61 C518 CYCLOFOS™-7, ANAGRADE® 35 F310 FOS-CHOLINE®-13, ANAGRADE® 61 C610 CYPFOS™-3, ANAGRADE® 43 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 121 Index Catalog Number Index (continued) Cat. # Product Name Page # Cat. # Product Name Page # ® ® F310S FOS-CHOLINE -13, SOL-GRADE 62 O311T n-Octyl-d17−β−D-glucopyranoside 84 F312 FOS-CHOLINE®-14, ANAGRADE® 62 O312 n-Octyl−β−D-galactopyranoside, ANAGRADE® 82 F312D FOS-CHOLINE®-14, DEUTERATED 62 O314 n-Octyl−β−D-thioglucopyranoside, ANAGRADE® 86 F312S FOS-CHOLINE®-14, SOL-GRADE® 63 O314LA n-Octyl−β−D-thioglucopyranoside, ANAGRADE® 86 F314 FOS-CHOLINE®-15, ANAGRADE® 63 O320 n-Octyl−β−D-thiomaltopyranoside, ANAGRADE® 86 F314S FOS-CHOLINE®-15, SOL-GRADE® 63 O330 Octaethylene glycol monododecyl ether, ANAGRADE® 81 F316 FOS-CHOLINE®-16, ANAGRADE® 64 O330A Octaethylene glycol monododecyl ether, Analytical Grade 81 F316S FOS-CHOLINE®-16, SOL-GRADE® 64 O355 Octadecyltrimethylammonium chloride 81 FCI09 FOS-CHOLINE®-ISO-9, ANAGRADE® 64 P300 PLURONIC® F-68 87 FCI11 FOS-CHOLINE®-ISO-11, ANAGRADE® 65 P305 PLURONIC® F-127 87 FCI116 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® 65 P310 2-Propyl-1-pentyl maltopyranoside, ANAGRADE® 90 FCU110 FOS-CHOLINE®-UNSAT-11-10, ANAGRADE® 65 P340 Pentaethylene glycol monodecyl ether, ANAGRADE® 87 FF360 FOSFEN™-9, ANAGRADE® 67 P350 Pentaethylene glycol monooctyl ether, ANAGRADE® 87 G1000 Guanidine hydrochloride 67 P400 Polyethylene glycol 400 89 H108 HEGA®-8, ANAGRADE® 67 P5008 PMAL™-C8 88 H109 HEGA®-9, ANAGRADE® 68 P5010 PMAL™-C10 88 H110 HEGA®-10, ANAGRADE® 68 P5012 PMAL™-C12 88 PRODUCT INFOMATION H111 HEGA®-11, ANAGRADE® 68 P5016 PMAL™-C16 89 H300 n-Heptyl−β−D-glucopyranoside, ANAGRADE® 69 P6000 Polyethylene glycol 6000, Flakes 89 H300LA n-Heptyl−β−D-glucopyranoside, ANAGRADE® 69 P8000F Polyethylene glycol 8000, Flakes 89 H301 n-Heptyl−β−D-thioglucopyranoside, ANAGRADE® 70 P8000P Polyethylene glycol 8000, Powder 89 H301LA n-Heptyl−β−D-thioglucopyranoside, ANAGRADE® 70 S110MT Sodium (2-sulfonatoethyl) methanethiosulfonate 92 H305 n-Hexyl−β−D-glucopyranoside, ANAGRADE® 72 S300 Sodium dodecanoyl sarcosine, ANAGRADE® 91 H310 n-Hexyl−β−D-maltopyranoside, ANAGRADE® 72 S300S Sodium dodecanoyl sarcosine, SOL-GRADE® 92 H320 n-Hexadecyl−β−D-maltopyranoside, ANAGRADE® 70 S350 Sucrose monododecanoate, ANAGRADE® 93 H350 Hexaethylene glycol monooctyl ether, ANAGRADE® 71 S1001 Sodium dodecyl sulfate, Biotech Grade 92

NEW PRODUCTS H355 Hexadecyltrimethylammonium chloride 71 S1010 Sodium cholate, ANAGRADE® 91 H360 Hexaethylene glycol monodecyl ether, Analytical Grade 71 S1010S Sodium cholate, SOL-GRADE® 91 I1002 Isopropyl−β−D-thiogalactopyranoside, ANAGRADE® 72 S2000 L-(+)-Selenomethionine, Analytical Grade 90 I1003 Isopropyl−β−D-thiogalactopyranoside, Analytical 73 S2033 Sodium taurocholate, ANAGRADE® 93 I1003P Isopropyl−β−D-thiogalactopyranoside, Analytical 73 T110MT [2-(Trimethylammonium)ethyl] methane thiosulfonate bromide 97 L360S LAPAO, SOL-GRADE® 74 T305 n-Tetradecyl-N,N-dimethylglycine, ANAGRADE® 94 L1000 LB Broth, Ready-Made Powder 73 T315 n-Tetradecyl−β−D-maltopyranoside, ANAGRADE® 94 L1001 Luria Broth, Ready-Made Powder 74 T315S n-Tetradecyl−β−D-maltopyranoside, SOL-GRADE® 95 L1002 LDS 74 T323 n-Tridecyl−β−D-maltopyranoside, ANAGRADE® 96 M110MT MMTS 76 T323LA n-Tridecyl−β−D-maltopyranoside, ANAGRADE® 96 M319 MEGA-8, ANAGRADE® 75 T323S n-Tridecyl−β−D-maltopyranoside, SOL-GRADE® 97 ® ® PRODUCT LISTING M320 MEGA-10, ANAGRADE 76 T350 Tetraethylene glycol monooctyl ether, ANAGRADE 95 M325 MEGA-9, ANAGRADE® 75 T355 Tetradecyltrimethylammonium chloride 95 M2500 Micropipettes 76 T360 n-Tetradecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 94 N150 NOPOL-FOS™, ANAGRADE® 80 T370 TRIPAO 97 N324 n-Nonyl−β−D-glucopyranoside, ANAGRADE® 78 T1000 TCEP 93 N324LA n-Nonyl−β−D-glucopyranoside, ANAGRADE® 78 T1001 TRITON® X-100 98 N324S n-Nonyl−β−D-glucopyranoside, SOL-GRADE® 79 T1002 TRITON® X-114 98 N330 n-Nonyl−β−D-maltopyranoside, ANAGRADE® 79 T1003 TWEEN® 20 98 N335 n-Nonyl−β−D-thioglucopyranoside, ANAGRADE® 80 T1004 TWEEN® 80 99 N350 n-Nonyl−β−D-thiomaltopyranoside, ANAGRADE® 80 T1005 TWEEN® 40 98 ND195 NDSB-195 76 T1006 TWEEN® 60 99 ND201 NDSB-201 77 U300 n-Undecyl−β−D-maltopyranoside, ANAGRADE® 100 ND211 NDSB-211 77 U300HA n-Undecyl−α−D-maltopyranoside, ANAGRADE® 100 ND221 NDSB-221 77 U300LA n-Undecyl−β−D-maltopyranoside, ANAGRADE® 101 ND256 NDSB-256 77 U300S n-Undecyl−β−D-maltopyranoside, SOL-GRADE® 101 NIDP40 Nonidet P40 Substitute 77 U310 ω-Undecylenyl−β−D-maltopyranoside, ANAGRADE® 102 O110MT MTSL 76 U342 n-Undecyl−β−D-thiomaltopyranoside, ANAGRADE® 102 O310 n-Octyl−β−D-maltopyranoside, ANAGRADE® 85 U360 n-Undecyl-N,N-dimethylamine-oxide, ANAGRADE® 99 O310S n-Octyl−β−D-maltopyranoside, SOL-GRADE® 85 U1000 Urea 103 ®

INDEX O311 n-Octyl−β−D-glucopyranoside, ANAGRADE 82 O311HA n-Octyl−α−D-glucopyranoside, ANAGRADE® 83

O311D n-Octyl-d17−β−D-glucopyranoside-d7 84 O311S n-Octyl−β−D-glucopyranoside, SOL-GRADE® 83

122 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION INDEX

Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page # 2-Aminoethyl methanethiosulfonate hydrobromide A110MT 20 Carbenicillin disodium salt CA100 30 (S)-2-Amino-4-(methylseleno) butyric acid S2000 90 α-Carboxybenzylpenicillin CA100 30 Ampicillin, sodium salt A1000 20 CHAPS, ANAGRADE® C316 30 ANAMEG®-7, ANAGRADE® A340 20 CHAPSO, ANAGRADE® C317 31 ® ® ® C-HEGA -8, ANAGRADE C408 31

ANAPOE -20 APT020 21 PRODUCT INFORMATION ® ® ANAPOE®-35 APB035 21 C-HEGA -9, ANAGRADE C409 32 ® ® ANAPOE®-58 APB058 21 C-HEGA -10, ANAGRADE C410 32 ® ® ANAPOE®-80 APT080 22 C-HEGA -11, ANAGRADE C411 32 ® 3-[(3-Cholamidopropyl)dimethylammonio]-2-hydroxy-1- ANAPOE -C10E6 APO106 22 ® propanesulfonate C317 31 ANAPOE -C10E9 APO109 22 ® 3-[(3-Cholamidopropyl)-dimethylammonio]-1-propane sulfonate C316 30 ANAPOE -C12E8 APO128 23 ® 5-Cholesten-3b-ol CH200 33 ANAPOE -C12E9 APO129 23 ® Cholesterol CH200 33 ANAPOE -C12E10 AP1210 23 ® Cholesteryl hemisuccinate tris salt CH210 33 ANAPOE -C13E8 APO138 23 ANAPOE®-NID-P40 APND40 24 Cleland's Reagent D2000 56 ® ANAPOE®-X-100 APX100 24 Cleland's Reagent, ANAGRADE D2000A 56 ANAPOE®-X-114 APX114 24 CMC dye solution C3000 33 ANAPOE®-X-305 APX305 25 Culture tubes C6500 33 NEW PRODUCTS ANAPOE®-X-405 APX405 25 Cyclododecyl-1-phosphocholine C640 29 ™ ® 8-Anilino-1-napthalene sulfonic acid C3000 33 CYCLOFOS -2, ANAGRADE C508 34 CYCLOFOS™-3, ANAGRADE® C510 34 ANZERGENT® 3-8, Analytical Grade AZ308 25 CYCLOFOS™-4, ANAGRADE® C512 34 ® ANZERGENT 3-10, Analytical Grade AZ310 26 CYCLOFOS™-5, ANAGRADE® C514 35 ANZERGENT® 3-12, Analytical Grade AZ312 26 CYCLOFOS™-6, ANAGRADE® C516 35 ANZERGENT® 3-14, Analytical Grade AZ314 27 CYCLOFOS™-7, ANAGRADE® C518 35 4-Cyclohexyl-1-butyl D-glucoside C324G 37 ANZERGENT® 3-16, Analytical Grade AZ316 27 −β− 4-Cyclohexyl-1-butyl−β−D-maltoside C324 39 ® ANZERGENT 3-18, Analytical Grade AZ318 28 4-Cyclohexyl-1-butylphosphocholine C512 34 APO-8 D328 49 2-Cyclohexyl-1-ethyl−β−D-maltoside C322 38 APO-10 D330 48 2-Cyclohexyl-1-ethylphosphocholine C508 34 Big CHAP, Analytical Grade B300 28 7-Cyclohexyl-1-heptyl−β−D-maltoside C327 42 Big CHAP, Deoxy, Analytical Grade B310 28 7-Cyclohexyl-1-heptylphosphocholine C518 35 N,N'-bis-(3-D-Gluconamidopropyl) cholamide B300 28 6-Cyclohexyl-1-hexyl−β−D-maltoside C326 40 N,N'-bis-(3-D-Gluconamidopropyl) deoxycholamide B310 28 6-Cyclohexyl-1- hexylphosphocholine C516 35 Blue-GAL B2040 29 Cyclohexylmethyl−β−D-maltoside C321 37 ® BRIJ -35 APB035 21 5-Cyclohexyl-1-pentyl−β−D-maltoside C325 39 BRIJ®-58 APB058 21 5-Cyclohexyl-1-pentylphosphocholine C514 35 5-Bromo-4-chloro-3-indolyl−β−D-galactopyranoside B2000 103 3-Cyclohexyl-1-propyl−β−D-glucoside C323G 36 5-Bromo-6-chloro-3-indolyl−β−D-galactopyranoside B2010 75 3-Cyclohexyl-1-propyl−β−D-maltoside C323 38 5-Bromo-4-chloro-3-indolyl−β−D-glucuronic acid, 3-Cyclohexyl-1-propylphosphocholine C510 34 cyclohexyl ammonium salt B2020 104 Cyclohexylbutanoyl-N-hydroxyethylglucamide C410 32 5-Bromo-4-chloro-3-indolyl−β−D-glucuronic Acid, Cyclohexylethanoyl-N-hydroxyethylglucamide C408 31 sodium salt B2030 104 Cyclohexylpentanoyl-N-hydroxyethylglucamide C411 32 5-Bromo-3-indolyl−β−D-galactopyranoside B2040 29 Cyclohexylpropanoyl-N-hydroxyethylglucamide C409 32 3-(3-Butyl-3-phenylheptanamido)-N,N-dimethylpropan- Cyclooctyl-1-phosphocholine C630 29 1-amine oxide T370 97 3-Cyclopentyl-1-propylphosphocholine C610 43 ® ™ ® C8E4, ANAGRADE T350 95 CYGENT 1-3, ANAGRADE C702 36 ® ® ® C8E5, ANAGRADE P350 87 CYGLU -3, ANAGRADE C323G 36 ® ® ® C8E6, ANAGRADE H350 71 CYGLU -4, ANAGRADE C324G 37 ® ® ® C10E5, ANAGRADE P340 87 CYMAL -1, ANAGRADE C321 37 ® ® C10E6, Analytical Grade H360 71 CYMAL -2, ANAGRADE C322 38 ® ® ® C12E8, ANAGRADE O330 81 CYMAL -3, ANAGRADE C323 38 INDEX ® ® C12E8, Analytical Grade O330A 81 CYMAL -4, ANAGRADE C324 39 ® ® C12E23 APB035 21 CYMAL -5, ANAGRADE C325 39 ® ® C16E20 APB058 21 CYMAL -5, SOL-GRADE C325S 40 C-DODECAFOS™, ANAGRADE® C640 29 CYMAL®-6, ANAGRADE® C326 40 ™ ® C-OCTAFOS , ANAGRADE C630 29 Product Name/Chemical Name Cat. # Page #

800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com 123 Index INDEX (continued)

CYMAL®-6, ANAGRADE® C326LA 41 DTT, Cleland's Reagent D2000 56 CYMAL®-6, SOL-GRADE® C326S 41 DTT, Cleland's Reagent, ANAGRADE® D2000A 56 CYMAL®-7, ANAGRADE® C327 42 EDTA E1000 57 CYMAL®-7, SOL-GRADE® C327S 42 EGTA E1001 57 CYPFOS™-3, ANAGRADE® C610 43 Empigen BB® D350 50 DDAO D360 49 Ethylene diaminetetracetic acid, disodium salt, dihydrate E1000 57 DDAO D360S 50 Ethylene glycol-O,O'-bis(2-aminoethyl-N,N,N',N'-tetracetic acid) E1001 57 Decanoyl-N-hydroxyethylglucamide H110 68 N-Ethylmaleimide (NEM) E2000 57 Decanoyl-N-methylglucamide M320 76 FOS-CHOLINE®-8, ANAGRADE® F300 58 3,6,9,12,15,18,24,27,30-Decaoxadotetracontan-1-ol AP1210 23 FOS-CHOLINE®-8, SOL-GRADE® F300S 58 n-Decyl-N,N-dimethylamine-N-oxide, ANAGRADE® D365 43 FOS-CHOLINE®-9, ANAGRADE® F302 58 n-Decyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ310 26 FOS-CHOLINE®-9, SOL-GRADE® F302S 59 n-Decyl-N,N-dimethylglycine, ANAGRADE® D352 43 FOS-CHOLINE®-10, ANAGRADE® F304 59 Decyldimethyl-phosphine oxide D330 48 FOS-CHOLINE®-10, SOL-GRADE® F304S 59 n-Decyl−β−D-glucopyranoside, ANAGRADE® D321 44 FOS-CHOLINE®-11, ANAGRADE® F306 60 n-Decyl−β−D-glucoside D321 44 FOS-CHOLINE®-11, SOL-GRADE® F306S 60 PRODUCT INFOMATION Decyl hexaethylene glycol ether H360 71 FOS-CHOLINE®-12, ANAGRADE® F308 60 Decyl hexaglycol H360 71 FOS-CHOLINE®-12, DEUTERATED F308D 61 α-Decyl-ω-hydroxy-poly(oxy-1,2-ethanediyl) APO109 22 FOS-CHOLINE®-12, SOL-GRADE® F308S 61 n-Decyl−β−D-maltopyranoside, ANAGRADE® D322 44 FOS-CHOLINE®-13, ANAGRADE® F310 61 n-Decyl−α−D-maltopyranoside, ANAGRADE® D322HA 45 FOS-CHOLINE®-13, SOL-GRADE® F310S 62 n-Decyl−β−D-maltopyranoside, ANAGRADE® D322LA 45 FOS-CHOLINE®-14, ANAGRADE® F312 62 n-Decyl−β−D-maltopyranoside, SOL-GRADE® D322S 46 FOS-CHOLINE®-14, DEUTERATED F312D 62 n-Decyl−β−D-maltoside, ANAGRADE® D322 44 FOS-CHOLINE®-14, SOL-GRADE® F312S 63 n-Decyl−α−D-maltoside, ANAGRADE® D322HA 45 FOS-CHOLINE®-15, ANAGRADE® F314 63 NEW PRODUCTS n-Decyl−β−D-maltoside, ANAGRADE® D322LA 45 FOS-CHOLINE®-15, SOL-GRADE® F314S 63 n-Decyl−β−D-maltoside, SOL-GRADE® D322S 46 FOS-CHOLINE®-16, ANAGRADE® F316 64 Decyl pentaethylene glycol ether P340 87 FOS-CHOLINE®-16, SOL-GRADE® F316S 64 Decylpentaglycol P340 87 FOS-CHOLINE®-ISO-9, ANAGRADE® FCI09 64 n-Decyl−β−D-thioglucopyranoside, ANAGRADE® D323 46 FOS-CHOLINE®-ISO-11, ANAGRADE® FCI11 65 n-Decyl−β−D-thioglucoside D323 46 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® FCI116 65 n-Decyl−β−D-thiomaltopyranoside, ANAGRADE® D335 47 FOS-CHOLINE®-UNSAT-11-10, ANAGRADE® FCU110 65 n-Decyl−β−D-thiomaltoside D335 47 FOS-MEA®-8, ANAGRADE® F208 66 Decylphospho-n-methylethanolamine F210 66 FOS-MEA®-10, ANAGRADE® F210 66 n-Decylphosphocholine, ANAGRADE® F304 59 FOS-MEA®-12, ANAGRADE® F212 66 n-Decylphosphocholine, SOL-GRADE® F304S 59 FOSFEN™-9, ANAGRADE® FF360 67 PRODUCT LISTING Decyltrimethylammonium chloride D354 47 β−D-Fructopyranosyl−α−D-glucopyranoside monododecanoate S350 93 Deoxycholic acid, sodium salt, ANAGRADE® D380 47 Guanidine hydrochloride G1000 67 DIFOS™-12, ANAGRADE® D412 48 HECAMEG A340 20 3a,12a-Dihydroxy-5b-cholan-24-oic acid, monosodium salt D380 47 HEGA®-8, ANAGRADE® H108 67 Dimethylbenzlammoniumpropanesulfonate ND256 77 HEGA®-9, ANAGRADE® H109 68 Dimethylethylammoniumpropanesulfonate ND195 76 HEGA®-10, ANAGRADE® H110 68 Dodecyl−β−D-maltoside, ANAGRADE® D310A 53 HEGA®-11, ANAGRADE® H111 68 n-Dodecyl−β−D-maltoside, ANAGRADE® D310LA 53 6-O-(N-Heptylcarbamoyl)-methyl−α−D-glucopyranoside A340 20 n-Dodecyl−β−D-maltoside, SOL-GRADE® D310S 54 n-Heptyl−β−D-glucopyranoside, ANAGRADE® H300 69

n-Dodecyl-d25−β−D-maltoside D310T 54 n-Heptyl−β−D-glucoside H300 69 Dodecyl octaethylene glycol ether O330 81 n-Heptyl−β−D-glucopyranoside, ANAGRADE® H300LA 69 Dodecyl octaglycol O330 81 n-Heptyl−β−D-glucoside H300LA 69 n-Dodecylphosphocholine, ANAGRADE® F308 60 n-Heptyl−β−D-thioglucopyranoside, ANAGRADE® H301 70 n-Dodecylphosphocholine, SOL-GRADE® F308S 61 n-Heptyl−β−D-thioglucoside H301 70 Dodecylphospho-n-methylethanolamine F212 66 n-Heptyl−β−D-thioglucopyranoside, ANAGRADE® H301LA 70 n-Dodecyl−β−D-thiomaltopyranoside, ANAGRADE® D342 55 n-Heptyl−β−D-thioglucoside H301LA 70 n-Dodecyl−β−D-thiomaltoside D342 55 n-Hexadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ316 27 Dodecyltrimethylammonium chloride D355 55 α-Hexadecyl-ω-hydroxy-poly(oxy-1,2-ethanediyl) APB058 21 DTE D2001 55 n-Hexadecyl−β−D-maltopyranoside, ANAGRADE® H320 70 INDEX DTNB D1000 56 n-Hexadecyl−β−D-maltoside H320 70 Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page #

124 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION INDEX (continued)

2-[[(Hexadecyloxy)hydroxyphosphinyl]oxy]-N,N,N-trimethyl- MTSL O110MT 76 ethanaminium hydroxide, inner salt F316 64 NDSB-195 ND195 76 n-Hexadecylphosphocholine, ANAGRADE® F316 64 NDSB-201 ND201 77 n-Hexadecylphosphocholine, SOL-GRADE® F316S 64 NDSB-211 ND211 77 Hexadecyltrimethylammonium chloride H355 71 NDSB-221 ND221 77 n-Hexaethylene glycol monodecyl ether, Analytical Grade H360 71 NDSB-256 ND256 77 PRODUCT INFORMATION n-Hexaethylene glycol monooctyl ether, ANAGRADE® H350 71 Nonanoyl-N-hydroxyethylgluamide H109 68 3,6,9,12,15,18-Hexaoxaoctacosan-1-ol APO106 22 Nonanoyl-N-methylglucamide M325 75 n-Hexyl−β−D-glucopyranoside, ANAGRADE® H305 72 Nonidet P40 Substitute NIDP40 77 n-Hexyl−β−D-glucoside H305 72 n-Nonyl−β−D-glucopyranoside, ANAGRADE® N324 78 n-Hexyl−β−D-maltopyranoside, ANAGRADE® H310 72 n-Nonyl−β−D-glucopyranoside, ANAGRADE® N324LA 78 n-Hexyl−β−D-maltoside H310 72 n-Nonyl−β−D-glucopyranoside, SOL-GRADE® N324S 79 3b-Hydroxy-5-cholestene CH200 33 n-Nonyl−β−D-glucoside, ANAGRADE® N324 78 Igepal CA-630 APND40 24 n-Nonyl−β−D-glucoside, SOL-GRADE® N324S 79 Igepal CA-630 NIDP40 77 n-Nonyl−β−D-maltopyranoside, ANAGRADE® N330 79 IPTG, ANAGRADE® I1002 72 n-Nonyl−β−D-maltoside N330 79 IPTG, Analytical Grade I1003 73 n-Nonyl−β−D-thioglucopyranoside, ANAGRADE® N335 80 IPTG, Analytical Grade I1003P 73 n-Nonyl−β−D-thioglucoside N335 80 Isopropyl−β−D-thiogalactopyranoside, ANAGRADE® I1002 72 n-Nonyl−β−D-thiomaltopyranoside, ANAGRADE® N350 80 NEW PRODUCTS Isopropyl−β−D-thiogalactopyranoside, Analytical Grade I1003 73 n-Nonyl−β−D-thiomaltoside N350 80 LAPAO, SOL-GRADE® L360S 74 Nonylphenylphosphocholine FF360 67 3-Laurylamido-N,N'-dimethylpropyl aminoxide L360S 74 n-Nonylphosphocholine F302 58 Lauroyl sarcosine S300 91 NOPOL-FOS™, ANAGRADE® N150 80 Lauroyl sucrose S350 93 n-Octadecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ318 28 Lauryl maltoside D310T 54 Octadecyltrimethylammonium chloride O355 81 Lauryl maltoside, ANAGRADE® D310 52 Octaethylene glycol monododecyl ether, ANAGRADE® O330 81 Lauryl maltoside, ANAGRADE® D310A 53 Octaethylene glycol monododecyl ether, Analytical Grade O330A 81 Lauryl maltoside, ANAGRADE® D310HA 52 Octanoyl-N-hydroxyethylglucamide H108 67 Lauryl maltoside, ANAGRADE® D310LA 53 Octanoyl-N-methylglucamide M319 75 Lauryl maltoside, SOL-GRADE® D310S 54 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol APO128 23 Lauryldimethylamine-N-oxide D360 49 3,6,9,12,15,18,21,24-Octaoxahexatriacontan-1-ol O330 81 Lauryldimethylamine-N-oxide D360S 50 n-Octyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ308 25 LB Broth, Ready-Made Powder L1000 73 Octyl dimethyl-phosphine oxide D328 49 LDAO D360 49 n-Octyl−β−D-galactopyranoside, ANAGRADE® O312 82 LDAO D360S 50 n-Octyl−β−D-galactoside O312 82 LDS L1002 74 n-Octyl−β−D-glucopyranoside, ANAGRADE® O311 82 Lithium dodecyl sulfate (LDS) L1002 74 n-Octyl−α−D-glucopyranoside, ANAGRADE® O311HA 83 Luria Broth, Ready-Made Powder L1001 74 n-Octyl−β−D-glucopyranoside, SOL-GRADE® O311S 83 Magenta-GAL B2010 75 n-Octyl-d17−β−D-glucopyranoside O311T 84 MEGA-8, ANAGRADE® M319 75 n-Octyl-d17−β−D-glucopyranoside-d7 O311D 84 ® MEGA-9, ANAGRADE M325 75 n-Octyl−β−D-glucoside, ANAGRADE® O311 82 ® MEGA-10, ANAGRADE M320 76 n-Octyl−α−D-glucoside, ANAGRADE® O311HA 83 Methanesulfonothioic acid, sodium salt S110MT 92 n-Octyl−β−D-glucoside, SOL-GRADE® O311S 83 1-Methylethyl-1-thio−β−D-galactopyranoside I1002 72 n-Octyl-d17−β−D-glucoside-d7 O311D 84 1-Methylethyl-1-thio−β−D-galactopyranoside I1003 73 n-Octyl-d17−β−D-glucoside, tail deuterated O311T 84 Methyl-6-O-(N-heptylcarbamoyl)−α−D-glucopyranoside A340 20 n-Octyl−β−D-maltopyranoside, ANAGRADE® O310 85 Methyl methanethiosulfonate M110MT 76 n-Octyl−β−D-maltopyranoside, SOL-GRADE® O310S 85 Methyl-oxirane, polymer with oxirane P300 87 n-Octyl−β−D-maltoside, ANAGRADE® O310 85 Methyl-oxirane, polymer with oxirane P305 87 n-Octyl−β−D-maltoside, SOL-GRADE® O310S 85 N-Methyl-N-(1-oxododecyl)-glycine, sodium salt S300 91 Octyl pentaethylene glycol ether P350 87 3-(1-Methylpiperidinium)-1-propane sulfonate ND221 77 Octylpentaglycol P350 87 Micropipettes M2500 76 [Octylphenoxyl]polyethoxyethanol APND40 24

MMTS M110MT 76 [Octylphenoxyl]polyethoxyethanol NIDP40 77 INDEX Monosodium-N-lauryl−β−iminodipropionate D346 51 n-Octylphosphocholine F300 58 MTSEA A110MT 20 Octylphospho-n-methylethanolamine F208 66 MTSES S110MT 92 Octyl tetraethylene glycol ether T350 95 MTSET T110MT 97 Octyltetraglycol T350 95 Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page #

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n-Octyl−β−D-thioglucopyranoside, ANAGRADE® O314 86 Sarkosyl S300 91 n-Octyl−β−D-thioglucoside O314 86 SDS S1001 92 n-Octyl−β−D-thioglucopyranoside, ANAGRADE® O314LA 86 L-(+)-Selenomethionine, Analytical Grade S2000 90 n-Octyl−β−D-thioglucoside O314LA 86 Sodium cholate, ANAGRADE® S1010 91 n-Octyl−β−D-thiomaltopyranoside, ANAGRADE® O320 86 Sodium cholate, SOL-GRADE® S1010S 91 n-Octyl−β−D-thiomaltoside O320 86 Sodium dodecanoyl sarcosine, ANAGRADE® S300 91 (1-Oxyl-2,2,5,5-tetramethyl-D3-pyrroline-3-methyl) Sodium dodecanoyl sarcosine, SOL-GRADE® S300S 92 methanethiosulfonate O110MT 76 Sodium dodecyl sulfate, Biotech Grade S1001 92 PEG 400 P400 89 Sodium lauroyl sarcosine, ANAGRADE® S300 91 PEG 6000 P6000 89 Sodium lauroyl sarcosine, SOL-GRADE® S300S 92 PEG 8000 P8000F 89 Sodium (2-sulfonatoethyl) methanethiosulfonate S110MT 92 PEG 8000 P8000P 89 Sodium taurocholate, ANAGRADE® S2033 93 n-Pentadecylphosphocholine F314 63 Sucrose monododecanoate, ANAGRADE® S350 93 Pentaethylene glycol monodecyl ether, ANAGRADE® P340 87 Sulfuric acid monododecyl ester sodium salt S1001 92 Pentaethylene glycol monooctyl ether, ANAGRADE® P350 87 1-(3-Sulfopropyl)-pyridinium hydroxide inner salt ND201 77 3,6,9,12,15-Pentaoxapentacosan-1-ol P340 87 TCEP T1000 93 PRODUCT INFOMATION 3,6,9,12,15-Pentaoxatricosan-1-ol P350 87 n-Tetradecyl-N,N-dimethyl-3-ammonio-1-propanesulfonate AZ314 27 8-(Phenylamino)-1-naphthalenesulfonic acid 3000 n-Tetradecyl-N,N-dimethylamine-N-oxide, ANAGRADE® T360 94 PLURONIC® F-68 P300 87 n-Tetradecyl-N,N-dimethylglycine, ANAGRADE® T305 94 PLURONIC® F-127 P305 87 Tetradecyl 4-O−α−D-glucopyranosyl−β−D-glucopyranoside T315 94 PMAL™-C8 P5008 88 n-Tetradecyl−β−D-maltopyranoside, ANAGRADE® T315 94 PMAL™-C10 P5010 88 n-Tetradecyl−β−D-maltopyranoside, SOL-GRADE® T315S 95 PMAL™-C12 P5012 88 n-Tetradecyl−β−D-maltoside, ANAGRADE® T315 94 PMAL™-C16 P5016 89 n-Tetradecyl−β−D-maltoside, SOL-GRADE® T315S 95 Poloxamer 188 P300 87 n-Tridecylphosphocholine, ANAGRADE® F310 61 NEW PRODUCTS Polyethylene glycol (23) monododecyl ether APB035 21 n-Tetradecylphosphocholine, ANAGRADE® F312 62 Polyethylene glycol (20) monohexadodecyl ether APB058 21 n-Tetradecylphosphocholine, Deuterated F312D 62 Poly (maleic anhydride-alt-1-decene) substituted with n-Tetradecylphosphocholine, SOL-GRADE® F312S 63 3-(Dimethylamino) propylamine P5008 88 Tetradecyltrimethylammonium chloride T355 95 Poly (maleic anhydride-alt-1-dodecene) substituted with Tetraethylene glycol monooctyl ether, ANAGRADE® T350 95 3-(Dimethylamino) propylamine P5010 88 α-[(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- Poly (maleic anhydride-alt-1-octadecene) substituted with poly(oxy-1,2-ethanediyl) APX114 24 3-(Dimethylamino) propylamine P5016 89 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- Poly (maleic anhydride-alt-1-tetradecene) substituted with poly(oxy-1,2-ethanediyl) APX100 24 3-(Dimethylamino) propylamine P5012 88 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate APT020 21 poly(oxy-1,2-ethanediyl) APX305 25 PRODUCT LISTING Poly(oxy-1,2-ethanediyl) derivs., sorbitan monododecanoate T1003 98 α-[4-(1,1,3,3-Tetramethylbutyl)phenyl]-ω-hydroxy- Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan poly(oxy-1,2-ethanediyl) APX405 25 mono-9-octadecanoate APT080 22 3,6,9,12-Tetraoxaeicosan-1-ol T350 95 Poly(oxy-1,2-ethanediyl) derivs., (Z)-sorbitan Thesit® APO129 23 mono-9-octadecanoate T1004 99 n-Tridecyl−β−D-maltopyranoside, ANAGRADE® T323 96 Polyoxyethylene(6)decyl ether APO106 22 n-Tridecyl−β−D-maltoside T323 96 Polyoxyethylene(9)decyl ether APO109 22 n-Tridecyl−β−D-maltopyranoside, ANAGRADE® T323LA 96 Polyoxyethylene(8)dodecyl ether APO128 23 n-Tridecyl−β−D-maltoside T323LA 96 Polyoxyethylene(9)dodecyl ether APO129 23 n-Tridecyl−β−D-maltopyranoside, SOL-GRADE® T323S 97 Polyoxyethylene(10)dodecyl ether AP1210 23 Tridecyl−β−D-maltoside T323S 97 Polyoxyethylene(8)tridecyl ether APO138 23 n-Tridecylphosphocholine, ANAGRADE® F310 61 Polyoxyethylene-polyoxypropylene block copolymer P300 87 n-Tridecylphosphocholine, SOL-GRADE® F310S 62 Polyoxyethylene-polyoxypropylene block copolymer P305 87 3a,7a,12a-Trihydroxy-5b-cholan-24-oic acid, monosodium salt S1010 91 Polyoxyethylene(20)sorbitan monolaurate APT020 21 3a,7a,12a-Trihydroxy-5b-cholan-24-oic acid, Polyoxyethylene(20)sorbitan monolaurate T1003 98 N-(2-sulfoethyl) amide S2033 93 Polyoxyethylene sorbitan monolaurate T1005 98 [2-(Trimethylammonium)ethyl] methane thiosulfonate Polyoxyethylene(80)sorbitan monolaurate APT080 22 bromide T110MT 97 Polyoxyethylene(80)sorbitan monolaurate T1004 99 TRIPAO T370 97 Polyoxyethylene sorbitan monooleate T1006 99 Tris (carboxyethyl) phosphine hydrochloride T1000 93 INDEX 2-propyl-1-pentyl−β−D-maltopyranoside, ANAGRADE® P310 90 TRITON® X-100 APX100 24 (3-1-Pyridino)-1-propane sulfonate ND201 77 TRITON® X-100 T1001 98 Product Name/Chemical Name Cat. # Page # Product Name/Chemical Name Cat. # Page #

126 800-252-1280 • 419-891-3030 • FAX 419-891-3037 • www.anatrace.com Index SERVICE INFORMATION INDEX (continued)

TRITON® X-114 APX114 24 TRITON® X-114 T1002 98 TRITON® X-305 APX305 25 TRITON® X-405 APX405 25 TWEEN® 20 APT020 21 TWEEN® 20 T1003 98 PRODUCT INFORMATION TWEEN® 40 T1005 98 TWEEN® 60 T1006 99 TWEEN® 80 APT080 22 TWEEN® 80 T1004 99 UDAO U360 99 Undecanoyl-N-hydroxyethylglucamide H111 68 Undecyldimethylamine-N-oxide U360 99 n-Undecyl-N,N-dimethylamine-oxide, ANAGRADE® U360 99 n-Undecyl−β−D-maltopyranoside, ANAGRADE® U300 100 n-Undecyl−α−D-maltopyranoside, ANAGRADE® U300HA 100 n-Undecyl−β−D-maltopyranoside, ANAGRADE® U300LA 101 ®

n-Undecyl−β−D-maltopyranoside, SOL-GRADE U300S 101 NEW PRODUCTS n-Undecyl−β−D-maltoside, ANAGRADE® U300 100 n-Undecyl−α−D-maltoside, ANAGRADE® U300HA 100 n-Undecyl−β−D-maltoside, ANAGRADE® U300LA 101 n-Undecyl−β−D-maltoside, SOL-GRADE® U300S 101 n-Undecyl−β−D-thiomaltopyranoside, ANAGRADE® U342 102 n-Undecyl−β−D-thiomaltoside, ANAGRADE® U342 102 ω-Undecylenyl−β−D-maltopyranoside, ANAGRADE® U310 102 ω-Undecylenyl−β−D-maltoside, ANAGRADE® U310 102 10-Undecylenyl-1-phosphocholine FCU110 65 n-Undecylphosphocholine, ANAGRADE® F306 60 n-Undecylphosphocholine, SOL-GRADE® F306S 60 Undecyl-6-phosphocholine FCI116 65 Urea U1000 103 X-GAL B2000 103 XGAL-IPTG B2050 103 X-Gluc, CHA salt B2020 104 X-Gluc, Na salt B2030 104 Zwittergent® 3-08 AZ308 25 Zwittergent® 3-10 AZ310 26 Zwittergent® 3-12 AZ312 26 Zwittergent® 3-14 AZ314 27 INDEX

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CAS # Cat. # Product Name Page #

57-13-6 U1000 Urea 103 57-88-5 CH200 Cholesterol 33 69-78-3 D1000 DTNB 56 82-76-8 C3000 CMC dye solution 33 69-52-3 A1000 Ampicillin, sodium salt 20 67-42-5 E1001 EGTA 57 50-01-1 G1000 Guanidine hydrochloride 67 128-53-0 E2000 NEM 57 145-42-6 S2033 Sodium taurocholate, ANAGRADE® 93 112-03-8 O355 Octadecyltrimethylammonium chloride 81 112-02-7 H355 Hexadecyltrimethylammonium chloride 71 112-00-5 D355 Dodecyltrimethylammonium chloride 55 137-16-6 S300 Sodium dodecanoyl sarcosine, ANAGRADE® 91 137-16-6 S300S Sodium dodecanoyl sarcosine, SOL-GRADE® 92 151-21-3 S1001 Sodium dodecyl sulfate, Biotech Grade 92 PRODUCT INFOMATION 302-95-4 D380 Deoxycholic acid, sodium salt, ANAGRADE® 47 367-93-1 I1002 Isopropyl−β−D-thiogalactopyranoside, ANAGRADE® 72 367-93-1 I1003 Isopropyl−β−D-thiogalactopyranoside, Analytical 73 367-93-1 I1003P Isopropyl−β−D-thiogalactopyranoside, Analytical 73 361-09-1 S1010 Sodium cholate, ANAGRADE® 91 361-09-1 S1010S Sodium cholate, SOL-GRADE® 91 683-10-3 D350 n-Dodecyl-N,N-dimethylglycine, ANAGRADE® 50 683-10-3 D350S n-Dodecyl-N,N-dimethylglycine, SOL-GRADE® 50 872-50-4 B2050 XGAL-IPTG 103 1643-20-5 D360 n-Dodecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 49 NEW PRODUCTS 1643-20-5 D360S n-Dodecyl-N,N-dimethylamine-N-oxide,SOL-GRADE® 50 1950-85-2 S110MT Sodium (2-sulfonatoethyl) methanethiosulfonate 92 2044-56-6 L1002 LDS 74 2190-95-6 D330 Dimethyldecylphosphine oxide, ANAGRADE® 48 2497-59-8 NIDP40 Nonidet P40 Substitute 77 2605-79-0 D365 n-Decyl-N,N-dimethylamine-N-oxide, ANAGRADE® 43 2601-33-4 T305 n-Tetradecyl-N,N-dimethylglycine, ANAGRADE® 94 2644-45-3 D352 n-Decyl-N,N-dimethylglycine, ANAGRADE® 43 2949-92-0 M110MT MMTS 76 ® 3055-98-9 APO128 ANAPOE -C12E8 23 3055-98-9 O330 Octaethylene glycol monododecyl ether, ANAGRADE® 81

PRODUCT LISTING 3055-98-9 O330A Octaethylene glycol monododecyl ether, Analytical Grade 81 ® 3055-99-0 APO129 ANAPOE -C12E9 23 3211-76-5 S2000 L-(+)-Selenomethionine, Analytical Grade 90 3332-27-2 T360 n-Tetradecyl-N,N-dimethylamine-N-oxide, ANAGRADE® 94 3655-00-3 D345 n-Dodecyl−β−iminodipropionic acid, disodium salt 51 4440-54-4 H350 Hexaethylene glycol monooctyl ether, ANAGRADE® 71 4574-07-3 T355 Tetradecyltrimethylammonium chloride 95 4800-94-6 CA100 Carbenicillin disodium salt 30 ® 5168-98-9 APO106 ANAPOE -C10E6 22 6381-92-6 E1000 EDTA 57 ® 6540-99-4 AP1210 ANAPOE -C12E10 23 6892-68-8 D2001 DTE 55 7240-90-6 B2000 X-GAL, ANAGRADE® 103 9004-95-9 APB058 ANAPOE®-58 21 9001-93-1 T1001 TRITON® X-100 98 9002-93-1 APX100 ANAPOE®-X-100 24 9002-93-1 APX305 ANAPOE®-X-305 25 9002-93-1 APX405 ANAPOE®-X-405 25 9002-92-0 APB035 ANAPOE®-35 21 9005-67-8 T1006 TWEEN® 60 99 INDEX 9005-66-7 T1005 TWEEN® 40 98

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CAS Registry Index (continued) CAS # Cat. # Product Name Page #

9005-65-6 APT080 ANAPOE®-80 22 9005-65-6 T1004 TWEEN® 80 99 9005-64-5 APND40 ANAPOE®—NID-P40 24 9005-64-5 APT020 ANAPOE®-20 21 ® 9005-64-5 T1003 TWEEN 20 98 PRODUCT INFORMATION 9003-11-6 P300 PLURONIC® F-68 87 9003-11-6 P305 PLURONIC® F-127 87 ® 9043-30-5 APO138 ANAPOE -C13E8 23 9036-19-5 APX114 ANAPOE®-X-114 24 9036-19-5 T1002 TRITON® X-114 98 10108-87-9 D354 Decyltrimethylammonium chloride 47 13232-68-3 D328 Dimethyloctylphosphine oxide 49 14565-56-4 D335 n-Decyl−β−D-thiomaltopyranoside, ANAGRADE® 47 14933-09-6 AZ314 ANZERGENT® 3-14, Analytical Grade 27 14933-08-5 AZ312 ANZERGENT® 3-12, Analytical Grade 26 14960-06-6 D346 n-Dodecyl−β−iminodipropionic acid, monosodium salt 51 15178-76-4 AZ308 ANZERGENT® 3-8, Analytical Grade 25

15178-71-9 U360 n-Undecyl-N,N-dimethylamine-oxide, ANAGRADE® 99 NEW PRODUCTS 15163-36-7 AZ310 ANZERGENT® 3-10, Analytical Grade 26 15471-17-7 ND201 NDSB-201 77 16599-33-0 A110MT 2-Aminoethyl methanethiosulfonate hydrobromide 20 18449-82-6 T315 n-Tetradecyl−β−D-maltopyranoside, ANAGRADE® 94 18449-82-6 T315S n-Tetradecyl−β−D-maltopyranoside, SOL-GRADE® 95 19327-40-3 P350 Pentaethylene glycol monooctyl ether, ANAGRADE® 87 19327-39-0 T350 Tetraethylene glycol monooctyl ether, ANAGRADE® 95 23244-49-7 P340 Pentaethylene glycol monodecyl ether, ANAGRADE® 87 25339-99-5 S350 Sucrose monododecanoate, ANAGRADE® 93 ® 26183-52-8 APO109 ANAPOE -C10E9 22 27565-41-9 D2000 DTT, Cleland’s Reagent 56 27565-41-9 D2000A DTT, Cleland’s Reagent, ANAGRADE® 56 29557-51-5 F308 FOS-CHOLINE®-12, ANAGRADE® 60 29557-51-5 F308S FOS-CHOLINE®-12, SOL-GRADE® 61 29781-80-4 O311HA n-Octyl−α−D-glucopyranoside, ANAGRADE® 83 29836-26-8 O311 n-Octyl−β−D-glucopyranoside, ANAGRADE® 83 29836-26-8 O311S n-Octyl−β−D-glucopyranoside, SOL-GRADE® 83 38880-58-9 ND211 NDSB-211 77 40427-75-6 O312 n-Octyl−β−D-galactopyranoside, ANAGRADE® 82 51805-45-9 T1000 TCEP 93 53255-89-3 F300 FOS-CHOLINE®-8, ANAGRADE® 58 53255-89-3 F300S FOS-CHOLINE®-8, SOL-GRADE® 58 58066-85-6 F316 FOS-CHOLINE®-16, ANAGRADE® 64 58066-85-6 F316S FOS-CHOLINE®-16, SOL-GRADE® 64 58846-77-8 D321 n-Decyl−β−D-glucopyranoside, ANAGRADE® 44 59080-45-4 H305 n-Hexyl−β−D-glucopyranoside, ANAGRADE® 72 59122-55-3 D318 n-Dodecyl−β−D-glucopyranoside, ANAGRADE® 51 61792-31-2 L360S LAPAO, SOL-GRADE® 74 69227-93-6 D310 n-Dodecyl−β−D-maltopyranoside, ANAGRADE® 52 69227-93-6 D310A n-Dodecyl−β−D-maltopyranoside, ANAGRADE® 53 69227-93-6 D310LA n-Dodecyl−β−D-maltopyranoside, ANAGRADE® 53 69227-93-6 D310S n-Dodecyl−β−D-maltopyranoside, SOL-GRADE® 54 69984-73-2 N324 n-Nonyl−β−D-glucopyranoside, ANAGRADE® 78 69984-73-2 N324LA n-Nonyl−β−D-glucopyranoside, ANAGRADE® 78 69984-73-2 N324S n-Nonyl−β−D-glucopyranoside, SOL-GRADE® 79 70504-28-8 F304 FOS-CHOLINE®-10, ANAGRADE® 59 INDEX 70504-28-8 F304S FOS-CHOLINE®-10, SOL-GRADE® 59 75621-03-3 C316 CHAPS, ANAGRADE® 30 75621-03-3 C316S CHAPS, SOL-GRADE® 31

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CAS Registry Index (continued) CAS # Cat. # Product Name Page #

77733-28-9 F312 FOS-CHOLINE®-14, ANAGRADE® 62 77733-28-9 F312S FOS-CHOLINE®-14, SOL-GRADE® 63 78617-12-6 H300 n-Heptyl−β−D-glucopyranoside, ANAGRADE® 69 78617-12-6 H300LA n-Heptyl−β−D-glucopyranoside, ANAGRADE® 69 81239-45-4 ND256 NDSB-256 77 82473-24-3 C317 CHAPSO, ANAGRADE® 31 82494-09-5 D322 n-Decyl−β−D-maltopyranoside, ANAGRADE® 44 82494-09-5 D322LA n-Decyl−β−D-maltopyranoside, ANAGRADE® 45 82494-09-5 D322S n-Decyl−β−D-maltopyranoside, SOL-GRADE® 46 82494-08-4 O310 n-Octyl−β−D-maltopyranoside, ANAGRADE® 85 82494-08-4 O310S n-Octyl−β−D-maltopyranoside, SOL-GRADE® 85 85316-98-9 M319 MEGA-8, ANAGRADE® 75 85261-20-7 M320 MEGA-10, ANAGRADE® 76 85261-19-4 M325 MEGA-9, ANAGRADE® 75 85618-21-9 O314 n-Octyl−β−D-thioglucopyranoside, ANAGRADE® 86 PRODUCT INFOMATION 85618-21-9 O314LA n-Octyl−β−D-thioglucopyranoside, ANAGRADE® 86 85618-20-8 H301 n-Heptyl−β−D-thioglucopyranoside, ANAGRADE® 70 85618-20-8 H301LA n-Heptyl−β−D-thioglucopyranoside, ANAGRADE® 70 85775-42-4 F310 FOS-CHOLINE®-13, ANAGRADE® 61 85775-42-4 F310S FOS-CHOLINE®-13, SOL-GRADE® 62 86303-23-3 B310 Big CHAP, Deoxy, Analytical Grade 28 86303-22-2 B300 Big CHAP, Analytical Grade 28 91774-25-3 T110MT [2-(Trimethylammonium)ethyl] methane thiosulfonate bromide 97 93863-88-8 B2010 Magenta-GAL 75 93911-12-7 T323 n-Tridecyl−β−D-maltopyranoside, ANAGRADE® 96 NEW PRODUCTS 93911-12-7 T323LA n-Tridecyl−β−D-maltopyranoside, ANAGRADE® 96 93911-12-7 T323S n-Tridecyl−β−D-maltopyranoside, SOL-GRADE® 97 97753-82-7 B2040 Blue-GAL 29 98064-96-1 H320 n-Hexadecyl−β−D-maltopyranoside, ANAGRADE® 70 98854-16-1 D323 n-Decyl−β−D-thioglucopyranoside, ANAGRADE® 46 98854-15-0 N335 n-Nonyl−β−D-thioglucopyranoside, ANAGRADE® 80 102601-49-0 CH210 Cholesteryl hemisuccinate tris salt 33 104702-33-2 F208 FOS-MEA®-8, ANAGRADE® 66 106402-05-5 N330 n-Nonyl−β−D-maltopyranoside, ANAGRADE® 79 114162-64-0 B2020 X-Gluc, CHA Salt 104 115457-83-5 A340 ANAMEG®-7, ANAGRADE® 20 PRODUCT LISTING 116183-64-3 D310HA n-Dodecyl−α−D-maltopyranoside, ANAGRADE® 52 121045-77-0 FCU110 FOS-CHOLINE®-UNSAT-11-10, ANAGRADE® 65 129274-39-1 F212 FOS-MEA®-12, ANAGRADE® 66

129522-81-2 O311T n-Octyl-d17−β−D-glucopyranoside 84 129541-41-9 B2030 X-Gluc, Na Salt 104 130890-78-7 F308D FOS-CHOLINE®-12, Deuterated 61 139361-84-5 H110 HEGA®-10, ANAGRADE® 68 146801-07-2 F314 FOS-CHOLINE®-15, ANAGRADE® 63 146801-07-2 F314S FOS-CHOLINE®-15, ANAGRADE® 63 148565-58-6 D342 n-Dodecyl−β−D-thiomaltopyranoside, ANAGRADE® 55 148565-57-5 U342 n-Undecyl−β−D-thiomaltopyranoside, ANAGRADE® 102 148565-55-3 N350 n-Nonyl−β−D-thiomaltopyranoside, ANAGRADE® 80 148616-91-5 O320 n-Octyl−β−D-thiomaltopyranoside, ANAGRADE® 86 160255-06-1 ND195 NDSB-195 76 160788-56-7 ND221 NDSB-221 77 168037-13-6 U300HA n-Undecyl−α−D-maltopyranoside, ANAGRADE® 100 168037-12-5 D322HA n-Decyl−α−D-maltopyranoside, ANAGRADE® 45 181135-57-9 C324 CYMAL®-4, ANAGRADE® 39 181135-58-0 C323 CYMAL®-3, ANAGRADE® 38 ® ®

INDEX 228579-27-9 C326 CYMAL -6, ANAGRADE 40 228579-27-9 C326LA CYMAL®-6, ANAGRADE® 41 228579-27-9 C326S CYMAL®-6, SOL-GRADE® 41

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CAS Registry Index (continued) CAS # Cat. # Product Name Page #

250692-65-0 C325 CYMAL®-5, ANAGRADE® 39 250692-65-0 C325S CYMAL®-5, SOL-GRADE® 40 253678-65-8 F306 FOS-CHOLINE®-11, ANAGRADE® 60 253678-65-8 F306S FOS-CHOLINE®-11, SOL-GRADE® 60 253678-64-7 F302 FOS-CHOLINE®-9, ANAGRADE® 58 PRODUCT INFORMATION 253678-64-7 F302S FOS-CHOLINE®-9, SOL-GRADE® 59 253678-67-0 U300 n-Undecyl−β−D-maltopyranoside, ANAGRADE® 100 253678-67-0 U300LA n-Undecyl−β−D-maltopyranoside, ANAGRADE® 101 253678-67-0 U300S n-Undecyl−β−D-maltopyranoside, SOL-GRADE® 101 260804-65-7 C322 CYMAL®-2, ANAGRADE® 38 260804-64-6 C321 CYMAL®-1, ANAGRADE® 37 349477-49-2 C327 CYMAL®-7, ANAGRADE® 42 349477-49-2 C327S CYMAL®-7, SOL-GRADE® 42 557788-85-9 F210 FOS-MEA®-10, ANAGRADE® 66 603111-75-7 C408 C-HEGA®-8, ANAGRADE® 31 657393-66-3 C518 CYCLOFOS™-7, ANAGRADE® 35 657393-65-2 C516 CYCLOFOS™-6, ANAGRADE® 35 657393-64-1 C514 CYCLOFOS™-5, ANAGRADE® 35 NEW PRODUCTS 675126-15-5 C512 CYCLOFOS™-4, ANAGRADE® 34 823796-66-3 C510 CYCLOFOS™-3, ANAGRADE® 34 823796-65-2 C508 CYCLOFOS™-2, ANAGRADE® 34

849110-74-3 D310T n-Dodecyl-d25−β−D-maltopyranoside 54 864434-16-2 C411 C-HEGA®-11, ANAGRADE® 32 864434-15-1 C410 C-HEGA®-10, ANAGRADE® 32 864434-14-0 C409 C-HEGA®-9, ANAGRADE® 32 869542-54-1 C324G CYGLU®-4, ANAGRADE® 37 869548-41-4 C610 CYPFOS™-3, ANAGRADE® 43 869549-38-2 C640 C-DODECAFOS™, ANAGRADE® 29 869550-29-8 C630 C-OCTAFOS™, ANAGRADE® 29 869541-00-4 C323G CYGLU®-3, ANAGRADE® 36 869638-98-2 F312D FOS-CHOLINE®-14, DEUTERATED 62 869648-98-6 FF360 FOSFEN™-9, ANAGRADE® 67 869646-90-2 FCI09 FOS-CHOLINE®-ISO-9, ANAGRADE® 64 869653-90-7 H109 HEGA®-9, ANAGRADE® 68 869647-81-4 FCI116 FOS-CHOLINE®-ISO-11-6U, ANAGRADE® 65 869660-84-4 N150 NOPOL-FOS™, ANAGRADE® 80 869647-65-4 FCI11 FOS-CHOLINE®-ISO-11, ANAGRADE® 65 869584-01-0 D412 DIFOS™-12, ANAGRADE® 48 869652-63-1 H108 HEGA®-8, ANAGRADE® 67 869666-57-9 O311D n-Octyl-d −β−D-glucopyranoside-d 84 17 7 869638-31-3 DH325 2,6-Dimethyl-4-heptyl−β−D-maltopyranoside, ANAGRADE® 48 869704-76-7 U310 ω-Undecylenyl−β−D-maltopyranoside, ANAGRADE® 102 869654-10-4 H111 HEGA®-11, ANAGRADE® 68 869668-28-0 P310 2-Propyl-1-pentyl maltopyranoside, ANAGRADE® 90 869796-48-5 C702 CYGENT™ 1-3, ANAGRADE® 36 869856-84-8 P5008 PMAL™-C8 88 869856-88-2 P5010 PMAL™-C10 88 869857-16-9 P5016 PMAL™-C16 89 869857-14-7 P5012 PMAL™-C12 88 870287-95-9 H310 n-Hexyl−β−D-maltopyranoside, ANAGRADE® 72 INDEX

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