DOCSLIB.ORG

Major Article Genotoxic Effects of Semi-Synthetic Isodillapiole on Oviposition in Aedes Aegypti (Linnaeus, 1762) (Diptera

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Major Article Genotoxic Effects of Semi-Synthetic Isodillapiole on Oviposition in Aedes Aegypti (Linnaeus, 1762) (Diptera Revista da Sociedade Brasileira de Medicina Tropical Journal of the Brazilian Society of Tropical Medicine Vol.:53:(e20200467): 2020 https://doi.org/10.1590/0037-8682-0467-2020 Major Article Genotoxic Effects of Semi-Synthetic Isodillapiole on Oviposition in Aedes aegypti (Linnaeus, 1762) (Diptera: Culicidae) Luiz Henrique Fonseca dos Santos[1], Pedro Rauel Cândido Domingos[2], Sabrina da Fonseca Meireles[3], Leticia Cegatti Bridi[3], [4] [3],[5] Ana Cristina da Silva Pinto and Míriam Silva Rafael [1]. Instituto Nacional de Pesquisas da Amazônia, Programa de Iniciação Científica, Manaus, AM, Brasil. [2]. Faculdade Uninassau de Manaus, Setor Saúde, Manaus, AM, Brasil. [3]. Instituto Nacional de Pesquisas da Amazônia, Programa de Pós-Graduação em Genética, Conservação e Biologia Evolutiva, Manaus, AM, Brasil. [4]. Universidade Federal do Amazonas, Programa de Pós-Graduação em Biotecnologia, Manaus, AM, Brasil. [5]. Instituto Nacional de Pesquisas da Amazônia, Coordenação de Sociedade, Ambiente e Saúde, Manaus, AM, Brasil. Abstract Introduction: Semi-synthetic dillapiole compounds derived from Piper aduncum essential oil are used as alternative insecticides to control insecticide-resistant Aedes aegypti. Thus, we aimed to evaluate the genotoxic effects of semi-synthetic isodillapiole on the nuclei of neuroblasts (larvae) and oocytes (females) and the mean oviposition rates of the females over four generations (G1, G2, G3, and G4) of Ae. aegypti. Methods: Larvae were captured in the city of Manaus, Amazonas state, Brazil, and exposed to isodillapiole in bioassays (20, 40, and 60 µg/mL) and a negative control (0.05% DMSO in tap water) for 4 h. The cerebral ganglia were extracted from the larvae and oocytes from the adult females to prepare slides for cytogenetic analysis. Breeding pairs were established and eggs counts were quantified taken after the bioassays. Results: The analysis of 20,000 interphase nuclei of neuroblasts and oocytes indicated significant genotoxicity (micronuclei, budding, polynucleated cells, and other malformations) compared to that of the control. Metaphasic and anaphasic nuclei presented chromosomal breaks; however, no significant variation and damage was observed in the negative control. A significant reduction in mean oviposition rates was also recorded following exposure to isodillapiole over the four generations (G1, G2, G3, and G4). Conclusions: The toxic and genotoxic effects of isodillapiole onAe. aegypti were caused by reduced oviposition in the females and nuclear abnormalities over the four generations of the trials. Further studies are required, rather than our in vitro assays, to verify the efficacy of exposure to this compound for controllingAe. aegypti. Keywords: Biological control. Dillapiole. Nuclear abnormality. Micronuclei. INTRODUCTION In recent decades, chemical insecticides, in particular temephos and deltamethrin, have been used in programs for the control of Aedes aegypti transmit the four dengue virus serotypes (DENV- Aedes mosquitos, such as Brazilian National Dengue Fever Control 1, DENV-2, DENV-3, and DENV-4), chikungunya, urban yellow Program (PNCD)6 . These authors argue that it has resulted in the fever, and the Zika virus1,2,3,4. Of these, dengue fever has had the development of resistance in these insects, which may be related greatest impact on human populations in recent decades. In Brazil, to a reduction in either the penetration rate of the insecticide or its 264,262 probable cases of dengue fever were reported in 2018, while metabolism by the insect6. This has led to increasing interest and in the USA, a total of 560,586 cases were reported5. research into the potential of essential oils and other compounds derived from plants as alternatives to insecticides for the control 7,8,9 Corresponding author: Dra. Míriam Silva Rafael. of mosquito disease vectors . e-mail: [email protected] Dillapiole is derived from the essential oil of the spiked https://orcid.org/0000-0003-4726-855X Received 18 July 2020 pepper plant Piper aduncum (Piperaceae), which has potential as 10 Accepted 23 October 2020 a bioinsecticide for the control of insect pests . This compound, www.scielo.br/rsbmt I www.rsbmt.org.br 1/7 Santos LHF et al. – Effects of Isodillapiole on Aedes aegypti which is abundant in the essential oil of P. aduncum, is a phenyl The collection of specimens was authorized by the Chico Mendes ether, and functions as a fungicide, bactericide, and molluscicide11,12. Institute for Biodiversity Conservation (ICMBio) and the Biodiversity Information and Authorization System (SISBIO), through the The semi-synthetic derivatives of dillapiole are isodillapiole, permanent license number: 32941 (May 21, 2012) for the collection methyl ethers, ethyl, propyl, butyl, and octil dillapiole, and of zoological material, issued to Dr. Míriam Silva Rafael. dillapiole epoxide, and these derivatives have adulticide effects in 13 Ae. aegypti . The variation in the activity of these compounds is Establishment of G1 colony of Ae. aegypti related to the differences in the dillapiole side chain, which directly influences its larvicidal effects inAe. aegypti, Anopheles darlingi, The larvae captured in the field were raised at 26°C with a and Culex quinquefasciatus13. relative humidity of 70% and a standard 12 h-12 h light-dark cycle. The larvae were provisioned twice a day with commercial fish food Dillapiole has toxic and genotoxic effects in the larvae and adult (Tetra cichlid) stored in tap water in enamel trays (20 cm × 7 cm). 8,13,14 15 mosquitos of Ae. aegypti and Ae. albopictus . The karyotype The water and fish food were changed three times a week, following of Ae. aegypti has two pairs of autosomes and one pair of sex the standard protocol of the insectarium. Once the adults emerged, 8,16 chromosomes with a diploid number of 2n = 6 . they were identified using the taxonomic key18. The scenario of disease transmission by Ae. aegypti has changed Pairs of adult mosquitos (n = 400) were maintained for 15 days in recent decades due to the emergence and reemergence of urban in cages adapted for mating and oviposition. The larvae emerging transmission cycles of different arboviruses by this invasive mosquito. from the resulting eggs were denominated as G1. Adult females In Ae. aegypti exposed to semi-synthetic derivatives of dillapiole (n = 200) from colony G1 were fed hamster (Mesocricetus aureatus) blood, at low concentrations, DNA damage and decreased fertility were and the males (n = 200) were fed 10% sugar solution in 40 × 40 cm2 screened observed over four successive generations, with further trials cages. This setup ensured the production of maximum number of being hampered by the difficulty of obtaining more generations eggs, under authorization number 020/2017 of the Ethics Committee of mosquitoes due to their infertility following exposure to toxic on the Use of Animals (CEUA) / INPA Central Bioterium. compounds8,14. We evaluated the genotoxic effects of isodillapiole in the brain ganglia and oocytes of Ae. aegypti to analyze possible Bioassays of Ae. aegypti larvae and adults chromosomal abnormalities to determine the frequency of changes Third-stage Ae. aegypti larvae of the G1 colony (n = 200) were associated with the decreased fertility of mosquitoes and their exposed to three different isodillapiole treatments (20, 40, and 60 descendants over four generations, as well as the potential of this µg/mL), which were diluted in 20 mL distilled water. The choice to compound as an alternative tool to control Ae. aegypti. use isodillapiole concentrations was based on the LC50 (minimum METHODS inhibitory concentration) value14, during our toxicity assay, which were necessary to guarantee the survival of the immature insect Production of semi-synthetic isodillapiole for the genotoxicity bioassays. The third-stage larvae were divided Flash Column Chromatography into five replicates, with 40 larvae in each concentration as well Isodillapiole, a semi-synthetic derivative of dillapiole, was as a negative control of 0.05% DMSO dissolved in tap water. The obtained by isomerization with a 17% solution of KOH in ethanol larvae were exposed for 4 h in all cases. Following the bioassay, ten under reflux for 24 h. Isodillapiole was purified in flash Column third-stage larvae from each concentration and the negative control Chromatography (CC) by elution with hexane; AcOEt (95:5) and were used for cytological preparations (mitotic chromosomes). MeOH17. The mixing of E and Z isomers, based on nuclear magnetic The surviving larvae were transferred to enamel containers with resonance spectroscopy13, was conducted at the Laboratory for water and fish feed for development until the adult phase. Ten adult Research in Natural Products (CPPN) of the National Institute of females from each group were used to prepare the slides for the Amazonian Research (INPA) in Manaus, Amazonas state, Brazil. retrieval of meiotic chromosomes from the ovaries. The surviving mosquito pairs (n = 10) were transferred to cages adapted for mating Capture of Ae. aegypti and oviposition. All procedural steps were repeated for each of the three subsequent generations (G , G , and G ). Larvae of Ae. aegypti were collected during the Amazonian rainy 2 3 4 season from January to March, 2019, both inside and surrounding Cytological preparations homes in the Centro neighborhood of the city of Manaus, the capital of the Brazilian state of Amazonas (03°08’33.5″” S, 60°01’13.5”″ W). A total of 320 specimens of cerebral ganglia of third stage
Load more

Recommended publications
  • Anethum Graveolens: an Indian Traditional Medicinal Herb and Spice
    P H C O G R E V . PLANT REVIEW Anethum graveolens: An Indian traditional medicinal herb and spice S. Jana, G. S. Shekhawat Department of Bioscience and Biotechnology, Banasthali University, Banasthali, Rajasthan, India Submitted: 22-03-10 Revised: **** Published: **** ABSTRACT Anethum graveolens L. (dill) has been used in ayurvedic medicines since ancient times and it is a popular herb widely used as a spice and also yields essential oil. It is an aromatic and annual herb of apiaceae family. The Ayurvedic uses of dill seeds are carminative, stomachic and diuretic. There are various volatile components of dill seeds and herb; carvone being the predominant odorant of dill seed and α-phellandrene, limonene, dill ether, myristicin are the most important odorants of dill herb. Other compounds isolated from seeds are coumarins, flavonoids, phenolic acids and steroids. The main purpose of this review is to understand the significance ofAnethum graveolens in ayurvedic medicines and non-medicinal purposes and emphasis can also be given to the enhancement of secondary metabolites of this medicinal plant. Key words: Anethum graveolens, ayurvedic uses, carvone, limonene, monoterpenes, review INTRODUCTION Middle Ages it was thought to protect against witchcraft. Greeks covered their heads with dill leaves to induce sleep. The genus name Anethum is derived from Greek word aneeson or aneeton, which means strong smelling. Its common use in BOTANICAL DESCRIPTION Ayurvedic medicine is in abdominal discomfort, colic and for promoting digestion. Ayurvedic properties of shatapushpa are Anethum graveolens L. is the sole species of the genus Anethum, katu tikta rasa, usna virya, katu vipaka, laghu, tiksna and snigdha though classified by some botanists in the related genus Peucedanum gunas.
    [Show full text]
  • Methyl Eugenol: Its Occurrence, Distribution, and Role in Nature, Especially in Relation to Insect Behavior and Pollination
    Journal of Insect Science: Vol. 12 | Article 56 Tan and Nishida Methyl eugenol: Its occurrence, distribution, and role in nature, especially in relation to insect behavior and pollination Keng Hong Tan1a* and Ritsuo Nishida2b 1Tan Hak Heng, 20, Jalan Tan Jit Seng, 11200 Penang, Malaysia 2Laboratory of Chemical Ecology, Graduate School of Agriculture, Kyoto University, Kyoto, 606-8502, Japan Abstract This review discusses the occurrence and distribution (within a plant) of methyl eugenol in different plant species (> 450) from 80 families spanning many plant orders, as well as various roles this chemical plays in nature, especially in the interactions between tephritid fruit flies and plants. Keywords: allomone, attractant, Bactrocera, chemical ecology, floral fragrance, insect pollinators, plant–insect interactions, plant semiochemicals, sex pheromone, synomone, tephritid fruit flies Abbreviations: ME, methyl eugenol; RK, raspberry ketone Correspondence: a [email protected], b [email protected], *Corresponding author Editor: Todd Shelly was editor of this paper. Received: 28 April 2011, Accepted: 27 August 2011 Copyright : This is an open access paper. We use the Creative Commons Attribution 3.0 license that permits unrestricted use, provided that the paper is properly attributed. ISSN: 1536-2442 | Vol. 12, Number 56 Cite this paper as: Tan KH, Nishida R. 2012. Methyl eugenol: Its occurrence, distribution, and role in nature, especially in relation to insect behavior and pollination. Journal of Insect Science 12:56 available online: insectscience.org/12.56 Journal of Insect Science | www.insectscience.org 1 Journal of Insect Science: Vol. 12 | Article 56 Tan and Nishida 1. Introduction ME has been successfully used in: a) fruit fly surveys (Tan and Lee 1982) and quarantine Plants produce a huge array of chemicals, detection (see reviews by Metcalf and Metcalf numbering tens of thousands, primarily for 1992; Vargas et al.
    [Show full text]
  • NVEO 2019, Volume 6, Special Issue
    NVEO 2019, Volume 6, Special Issue CONTENTS Wellcome address of the Presidents of the Local Organizing Committee......... 2 ISEO 2019 Committees....................................................................................... 3 ISEO Medal of Honour........................................................................................ 4 IFEAT - Young Scientists Fellowship.................................................................... 5 NVEO 2019 Editorial........................................................................................... 6 Scientific Programme......................................................................................... 8 List of Poster Presentations................................................................................ 12 Abstracts............................................................................................................. 18 Sponsors............................................................................................................. 186 50th International Symposium on Essential Oils (ISEO2019) Key of Abbreviations: WS workshop WL welcome lecture PL plenary lecture IS invited speaker OP oral presentation YS young scientist presentation PD panel discussion PP poster presentation YS PP young scientist poster presentation All abstracts are from the 50th International Symposium on Essential Oils (ISEO2019) Abstract Book By Editors: Johannes Novak & Iris Stappen are adapted to the NVEO –ISEO 2019 Special Issue e-ISSN: 2148-9637 Nat. Vol. & Essent. Oils, 2019, 6
    [Show full text]
  • Influence of Drying on the Chemical Composition and Bioactivity of Piper
    Research, Society and Development, v. 10, n. 8, e46810817397, 2021 (CC BY 4.0) | ISSN 2525-3409 | DOI: http://dx.doi.org/10.33448/rsd-v10i8.17397 Influence of drying on the chemical composition and bioactivity of Piper aduncum (Piperaceae) essential oil against Aedes aegypti (Diptera: Culicidae) Influência da secagem na composição química e bioatividade do óleo essencial de de Piper aduncum (Piperaceae) sobre Aedes aegypti (Diptera: Culicidae) Influencia del secado en la composición química y bioactividad del aceite esencial de Piper aduncum (Piperaceae) en Aedes aegypti (Diptera: Culicidae) Received: 06/20/2021 | Reviewed: 07/01/2021 | Accept: 07/05/2021 | Published: 07/16/2021 Thais Silva Santos ORCID: https://orcid.org/0000-0002-9325-1671 Universidade Federal de Goiás, Brazil E-mail: [email protected] Taynara Ellen Sardeiro Vieira ORCID: https://orcid.org/0000-0001-9976-9765 Universidade Federal de Goiás, Brazil E-mail: [email protected] José Realino de Paula ORCID: https://orcid.org/0000-0002-4424-7692 Universidade Federal de Goiás, Brazil E-mail: [email protected] Jerônimo Raimundo de Oliveira Neto ORCID: https://orcid.org/0000-0002-0261-4554 Universidade Federal de Goiás, Brazil E-mail: [email protected] Luiz Carlos da Cunha ORCID: https://orcid.org/0000-0002-1525-8528 Universidade Federal de Goiás, Brazil E-mail: [email protected] Adelair Helena dos Santos ORCID: https://orcid.org/0000-0003-1743-4297 Universidade Federal de Goiás, Brazil E-mail: [email protected] Camila Aline Romano ORCID: https://orcid.org/0000-0002-3564-6368 Universidade Federal de Goiás, Brazil E-mail: [email protected] Abstract Piper species are producers of essential oils with high yield and promising chemical composition for both perfumery and the pharmaceutical industry.
    [Show full text]
  • An Analysis of the Pharmacological Components of Herbal Teas Used for Galactagogue Effects by Gas Chromatography/Mass Spectromet
    Anadolu Klin / Anatol Clin Orijinal Makale/Original Article An Analysis of the Pharmacological Components of Herbal Teas Used for Galactagogue Effects by Gas Chromatography/Mass Spectrometry Galaktagog Etki İçin Kullanılan Bitkisel Çayların Farmakolojik Bileşenlerinin Gaz Kromatografisi/ Kütle Spektrometrisi ile Analizi Fidan Pesen Ozdogan1, Kemal Abstract Gokhan Ulusoy2, Seyfullah Oktay 1 3 Aim: This study aimed to analyze the phytochemical components of a number of herbs and Arslan , Enis Macit , Saliha Aysenur Cam1, Fatma Uysal1, herbal teas used to improve breast milk production by gas chromatography/mass spectrom- Muhammed Fatih Dogan1 etry (GC/MS). 1 Yıldırım Beyazıt University, Faculty Materials and Methods: The methanolic extracts were prepared by the maceration method us- of Medicine, Department of Medical ing a rotary evaporator. The essential oils were obtained by the Clevenger method. Pharmacology 2 Results: The GC/MS analyses of the essential oils and methanol extracts of the medicinal herbs Health Sciences University, Gulhane Faculty of Medicine, Department of Foeniculum vulgare, Pimpinella anisum, Trigonella foenum graceum, Urtica dioica, and Nigella Medical Pharmacology sativa, and their teas on the market were performed with high sensitivity. The chemical compo- 3 Health Sciences University, Gulhane nents identified were presented in detailed tables. Faculty of Medicine, Department of Toxicology Discussion and Conclusions: In our study possible pharmacological effects of the components identified were discussed in light
    [Show full text]
  • Antifungal Activity of Extracts, Essential Oil and Constituents from Petroselinum Crispum Against Colletotrichum Acutatum
    Research article http://www.revistas.unal.edu.co/index.php/refame Antifungal activity of extracts, essential oil and constituents from Petroselinum crispum against Colletotrichum acutatum Actividad antifúngica de extractos, aceite esencial y constituyentes de Petroselinum crispum contra Colletotrichum acutatum doi: 10.15446/rfnam.v71n3.68284 Rodrigo Pineda1, Samuel Vizcaíno2, Carlos M. García3, Jesús H. Gil 4 and Diego Durango3* ABSTRACT Keywords: The effect of extracts, essential oil, and their major constituents from parsley (Petroselinum crispum Apiaceae (Mill.) Fuss.) against the phytopathogenic fungus Collectotrichum acutatum was evaluated by the Parsley poisoned agar method. Results showed that all extracts, along with the essential oil, significantly inhibit Extracts the radial growth of C. acutatum at concentrations higher than 100 μg mL-1. The higher activity was Essential oil found for the essential oil followed by the n-hexane extract. Analysis by gas chromatography with mass GC-MS spectroscopy (GC-MS) of n-hexane extract and the essential oil of P. crispum showed that the major Parsley-apiole components correspond to the phenylpropanoids myristicin and parsley-apiole. Both compounds were isolated by conventional chromatographic techniques and their structures elucidated by spectroscopic methods. Myristicin and parsley-apiole displayed a significant inhibitory effect against C. acutatum. -1 The highest fungistatic activity was found to parsley-apiole with IC50 value of 40 μg mL . In conclusion, parsley may be a good source of antifungal compounds to control C. acutatum. RESUMEN Palabras clave: Se evaluó el efecto de extractos, el aceite esencial y los componentes principales del perejil Apiaceae (Petroselinum crispum (Mill.) Fuss.) contra el hongo fitopatógenoCollectotrichum acutatum mediante Perejil el método del agar envenenado.
    [Show full text]
  • Cloning and Expression of a Perilla Frutescens Cytochrome P450 Enzyme Catalyzing the Hydroxylation of Phenylpropenes
    plants Article Cloning and Expression of a Perilla frutescens Cytochrome P450 Enzyme Catalyzing the Hydroxylation of Phenylpropenes Mariko Baba 1 , Ken-ichi Yamada 2 and Michiho Ito 1,* 1 Department of Pharmacognosy, Graduate School of Pharmaceutical Sciences, Kyoto University, Sakyo, Kyoto 606-8501, Japan; [email protected] 2 Department of Pharmaceutical Organic Chemistry, Graduate School of Pharmaceutical Sciences, Tokushima University, Shomachi, Tokushima 770-8505, Japan; [email protected] * Correspondence: [email protected] Received: 14 April 2020; Accepted: 28 April 2020; Published: 1 May 2020 Abstract: Phenylpropanoid volatile components in plants are useful and valuable not only as flavorings, but also as medicines and food supplements. The pharmacological actions and toxicities of these compounds have been well studied but their synthetic pathways are generally unclear. In this study, we mined expressed sequence tag libraries of pure strains of perilla maintained for over 30 years for their oil type and conducted gas chromatography-mass spectrometry analyses of the perilla oils to confirm the presence of monohydrates speculated to be intermediates of the phenylpropene synthetics pathways. These putative monohydrate intermediates and their regioisomers were synthesized to identify the reaction products of assays of heterologously expressed enzymes. An enzyme involved in the synthesis of a phenylpropanoid volatile component was identified in perilla. Expression of this enzyme in Saccharomyces cerevisiae showed that it is a member of the cytochrome P450 family and catalyzes the introduction of a hydroxy group onto myristicin to form an intermediate of dillapiole. The enzyme had high sequence similarity to a CYP71D family enzyme, high regiospecificity, and low substrate specificity.
    [Show full text]
  • Essential Oils As Antimicrobial and Anti-Infectious Agents II”
    Supplementary Material for the Special Issue of Molecules “Essential Oils as Antimicrobial and Anti-infectious Agents II” Comparative evaluation of essential oils from medicinal-aromatic plants of Greece: Chemical composition, antioxidant capacity and antimicrobial activity against bacterial fish pathogens Thekla I. Anastasiou1, Manolis Mandalakis1,*, Nikos Krigas2, Thomas Vézignol1, Diamanto Lazari3, Pantelis Katharios1, Thanos Dailianis1, Efthimia Antonopoulou4 1 Institute of Marine biology, Biotechnology and Aquaculture, Hellenic Centre of Marine Research, 71500 Heraklion, Greece 2 Institute of Plant Breeding and Genetic Resources, Hellenic Agricultural Organization Demeter, P.O. Box 60458, 57001 Thermi, Thessaloniki, Greece 3 Laboratory of Pharmacognosy, School of Pharmacy, Faculty of Health Sciences, Aristotle University of Thessaloniki, 54124 Thessaloniki, Greece 4 Laboratory of Animal Physiology, Department of Zoology, School of Biology, Faculty of Sciences, Aristotle University of Thessaloniki, 54124, Greece * Corresponding author, E-mail address: [email protected], phone: +30-2810-337855, fax: +30-2810- 337822 (M. Mandalakis). 1 Table S1. Concentrations of individual chemical compounds detected in the 13 essential oils of the Mediterranean medicinal-aromatic plants under investigation. Lamiaceae Apiaceae Asteraceae b c No Compoundsa AId AIg Identificatione Classificationf Pennyroyal Pennyroyal Lavender Greek oregano sage Greek Rosemary Spanish oregano Savoury Lemon balm Fennel Rock samphire carrot Wild Chamomile 1 α-Thujene
    [Show full text]
  • Essential Oils of Leaves of Piper Species Display Larvicidal Activity Against the Dengue Vector, Aedes Aegypti (Diptera: Culicidae)
    105 Essential oils of leaves of Piper species display larvicidal activity against the dengue vector, Aedes aegypti (Diptera: Culicidae) 1Laboratório de Pesquisas Químicas em Produtos Naturais (LQPN), Departamento de Química, Universidade Federal de Rondônia (UNIR), Campus Universitário José Ribeiro Filho - BR 364, km 9,5 s/nº, Porto Velho, RO, Brasil; 2Laboratório de Bioecologia de Insetos (LABEIN), Departamento de Biologia, Universidade Federal de Rondônia (UNIR), Campus Universitário José Ribeiro Filho - BR 364, km 9,5 s/nº, Porto Velho, RO, Brasil; 3Fundação Oswaldo Cruz Rondônia (FIOCRUZ RO), Rua da Beira nº 7671, Bairro Lagoa, Porto Velho, RO, Brasil, CEP 76.812-245. *[email protected]; 4Centro de Estudos de Biomoléculas Aplicadas a Saúde (CEBIO), Departamento de Medicina, Universidade Federal de Rondônia (UNIR), Campus Universitário José Ribeiro Filho - BR 364, km 9,5 s/nº, Porto Velho, RO, Brasil ABSTRACT: The mosquito Aedes aegypti is the vector of the dengue virus, an endemic arbovirus from tropical and subtropical regions of the world. The increasing resistance of mosquitoes to commercial insecticides impairs regular control programs; therefore, chemical prospecting originating from the Amazonian flora is promising for potential new insecticides. Several Piper species are, notably, rich in phenylpropanoids and terpenoids, substances with proven insecticidal activity. The composition and the larvicidal activity of three Piper species against A. aegypti were evaluated. Essential oils were extracted by hydrodistillation in a modified Clevenger apparatus and analyzed by GC/MS. The major components found in Piper arboreum were germacrene D (31.83%) and bicyclogermacrene (21.40%); in Piper marginatum: (E)-methyl isoeugenol (27.08%), (E)-anethole (23.98%) and (Z)-methyl isoeugenol (12.01%); and in Piper aduncum: (E)-isocroweacin (29.52%) and apiole (28.62%) and elemicin (7.82%).
    [Show full text]
  • Redalyc.Antibacterial Activity of the Piper Aduncum Oil and Dillapiole, Its Main Constituent, Against Multidrug-Resistant Strain
    Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas ISSN: 0717-7917 [email protected] Universidad de Santiago de Chile Chile BRAZÃO, Maria Angélica B; BRAZÃO, Fabio V; MAIA, José Guilherme S; MONTEIRO, Marta C Antibacterial activity of the Piper aduncum oil and dillapiole, its main constituent, against multidrug-resistant strains Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas, vol. 13, núm. 6, 2014, pp. 517-526 Universidad de Santiago de Chile Santiago, Chile Available in: http://www.redalyc.org/articulo.oa?id=85632545002 How to cite Complete issue Scientific Information System More information about this article Network of Scientific Journals from Latin America, the Caribbean, Spain and Portugal Journal's homepage in redalyc.org Non-profit academic project, developed under the open access initiative © 2014 Boletín Latinoamericano y del Caribe de Plantas Medicinales y Aromáticas 13 (6): 517 - 526 ISSN 0717 7917 www.blacpma.usach.cl Artículo Original | Original Article Antibacterial activity of the Piper aduncum oil and dillapiole, its main constituent, against multidrug-resistant strains [Actividad antibacteriana del aceite de Piper aduncum y dillapiole, su componente principal, frente a cepas resistentes a múltiples fármacos] Maria Angélica B BRAZÃO1, Fabio V BRAZÃO2, José Guilherme S MAIA1 & Marta C MONTEIRO1 1Programa de Pós-Graduação em Ciências Farmacêuticas, Universidade Federal do Pará, Belém, PA, Brazil; 2Laboratório Ruth Brazão, 66060-220 Belém, PA, Brazil. Contactos | Contacts: Marta C MONTEIRO - E-mail address: [email protected] Abstract: The study aimed to evaluate the bactericidal activity of oil essential and dillapiole from P. aduncum against standard and multidrug-resistant strains of Staphylococcus spp.
    [Show full text]
  • Microwave-Assisted Extraction: an Alternative to Extract Piper Aduncum Essential Oil
    40639 Brazilian Journal of Development Microwave-assisted extraction: an alternative to extract Piper aduncum essential oil Extração assistida por micro-ondas: uma alternativa para a obtenção do óleo essencial de Piper aduncum DOI:10.34117/bjdv6n6-558 Recebimento dos originais: 11/05/2020 Aceitação para publicação: 24/06/2020 Lidiane Diniz do Nascimento Mestre em Engenharia Química pela Universidade Federal do Pará. Instituição: Museu Paraense Emílio Goeldi/ Universidade Federal do Pará. Endereço: Av. Perimetral, 1901, Terra Firme – Belém – PA – Brasil. ORCID: 0000-0003-1370-4472 E-mail: [email protected] Lorena Queiroz Almeida Graduanda em Engenharia Química pela Universidade Federal do Pará. Instituição: Universidade Federal do Pará. Endereço: Rua Augusto Corrêa, 01, Guamá – Belém – PA – Brasil. E-mail: [email protected] Erika Milene Pinto de Sousa Doutora em Engenharia de Recursos Naturais da Amazônia pela Universidade Federal do Pará. Instituição: Universidade Federal Rural da Amazônia. Endereço: Av. Barão de Capanema, s/n, Caixa D'Água – Capanema – PA – Brasil. E-mail: [email protected] Cristiane Maria Leal Costa Doutora em Engenharia Química pela Universidade Estadual de Campinas. Instituição: Universidade Federal do Pará. Endereço: Rua Augusto Corrêa, 01, Guamá – Belém – PA – Brasil. E-mail: [email protected] Kauê Santana da Costa Doutorando em Biotecnologia pela Universidade Federal do Pará. Instituição: Universidade Federal do Oeste do Pará. Endereço: Rua Vera Paz, s/n (Unidade Tapajós), Salé – Santarém – PA – Brasil. ORCID: 0000-0002-2735-8016 E-mail: [email protected] Eloisa Helena de Aguiar Andrade Doutora em Química pela Universidade Federal do Pará. Instituição: Museu Paraense Emílio Goeldi/ Universidade Federal do Pará.
    [Show full text]
  • Chemical Composition of the Essential Oil from Leaves and Flowering Aerial Parts of Psammogeton Canescens (DC.) Vake from Iran
    Journal of Medicinal Plants Chemical Composition of the Essential Oil from Leaves and Flowering Aerial Parts of Psammogeton canescens (DC.) Vake from Iran Rahimi-Nasrabadi M (PhD Student)1,2*, Gholivand MB (Prof. )1, Batooli H (Ph.D.)3, Vatanara A (Ph.D.) 4 1- Department of Chemistry, Razi University, Kermanshah, Iran 2- Department of Chemistry, Imam Hossein University, Tehran, Iran 3- Isfahan Research Center of Natural Sources and Agriculture, Kashan Station, Kashan, Iran 4- Department of Pharmaceutics, School of Pharmacy, Tehran University of Medical Sciences, Tehran, Iran *Corresponding author: Department of Chemistry, Razi University, Kermanshah, Iran Tel: +98- 831-7243399, Fax: +98- 831- 4229172 E-mail: [email protected] Receive: 13 Jun 2009 Acceptance: 26 Aug. 2009 Abstract Background: The genus Psammogeton Edyew. which belong to the umbelliferae family, is found in central areas of Asia. This genus consists of six species widespread in the sandy dunes of deserts. Among the four species present in Iran, Pssamogeton canescens (DC.) vatke is distributed in regions of Iran central. Objective: The aim of this study is to determine the chemical composition of essential oil of Psammogeton canescens. Methods: The essential oil was extracted by hydrodistillation of dried aerial part of plant and Downloaded from jmp.ir at 11:12 +0330 on Wednesday September 29th 2021 analysed by GC and GC/MS. Results: Thirty constituents representing 99.7% of total oil have been identified. The main constituents of the oil were found to be β-bisabolene (33.35%), Apiole (28.34 %), α-Pinene (11.86%) and Dill apiole (8.17%). Other representative compounds were identified as β-Pinene (2.68%), Myrcene (2.62%) and sylvestrene (2.42%).
    [Show full text]