Level Biology. Basic and Simplified Revision Notes
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Systematic “A” level Biology. Basic and simplified Revision notes. SYSTEMATIC “A” LEVEL BIOLOGY. Basic and simplified revision notes. STANDARD TEACHING SYLABUS: 1. Cell biology or cytology………………………………………..………………………….2 Definition of cytology/cell biology, definition of the cell. Microscopy. light and electron microscopes their structure, mode of operation and comparison between them, microscope practical techniques. Cell theory, types of organism’s i.e prokaryotes and eukaryotes, comparison between Prokaryotes and eukaryotes, why cells are small? Structures of the cell, cell diversity.. Cell division. Types of cell division, events that occur during each type, comparison between them and the importance of each type. 2.Histology………………………………………………………………………………………..4 Definition, types of tissues, their structures and functions,adaptations of some tissues to suit their function. Levels of organization. ie unicellular level; tissue level; organ level; system level and organism level; advantages and disadvantages of being unicellular and multicelar organism; 3. Classification of living organisms…………………………………………………….65 Common terms used: classification, taxonomy, systematics, binomial nomenclature, dichotomous keys, taxonomic hierarchy, and five kingdom system: Animalia, Plantae, fungi, Protista and monera general characteristic of organismin each kingdom and the examples. 4. Transport of materials in living organisms………………………………………107 5. Chemicals of life………………………………………………………………………….150 DNA structure, RNA structure, DNA replication and protein synthesis; 6. Nutrition in plants and animals………………………………………………….....173 7. Gaseous exchange………………………………………………………………………..194 8. Respiration………………………………………………………………………..……....204 9. Excretion and osmoregulation…………………………………………………….…212 10. Homeostasis……………………………………………………………………………..228 11. Coordination in plants and animals………………………………………………239 12.Animal behaviour……………………………………………………………………….264 13. Support and movement in plant and animals…………………………………279 14. Reproduction in living organisms…………………………………………………291 15. Variation and genetics………………………………………………………………..324 16. Ecology…………………………………………………………………………………….377 17. Evolution………………………………………………………………………………….415 1 Systematic “A” level Biology. Basic and simplified Revision notes. Cytology/ cell biology Is a branch of biology that deals which deals with the study of the structure of cells using a microscope. The cell. A cell is the basic unit of life. It is the basic functional and structural unit of an organism. All living organisms no matter their size are made of cells. Cells are responsible for carrying out all body processes such as respiration, excretion, osmo regulation, lomotion, circulation etc in the body of the organism. Cells aggregate (unite) in groups to form body structures. When studying the detailed structure of the cell, an instrument called a microscope is used. The microscopes. The term microscope is derived from two Geek words; mikros meaning small and skopein meaning to see or to examine. Cells are studied using a microscope. There are two main types of microscopes: i) The light microscope. These are also divided into two: a simple microscope e.g a hand lens and a compound microscope with many lenses. ii) The electron microscope. The light microscope. This type of microscopes uses light to illuminate the specimen under investigation or that has been placed on the stage. The simplest form of a light microscope is the one with a single lens that has low power magnification. This is known as a hand lens (a magnifying lens).A compound microscope uses two types of lenses ie the eye piece and the objective lens. Light from specimen passes through the first lens called objective lens and produces a magnified image. This image acts as the object of the second lens the eye piece which magnifies it further. The structure of a light microscope 2 Systematic “A” level Biology. Basic and simplified Revision notes. The magnification in a compound microscope. Magnification is the number of times an image is made bigger or smaller compared to the to the object. The total magnification of both lenses is the product of objective lens and magnification and the eye piece lens magnification. For example if the eye piece has the magnification of X10 and the objective lens has X40, then the total magnification is; Total magnification = eye piece magnification X objective lens magnification. = (X10) x (X40) = X400. The resolution (resolving power) of the light microscope. This is the ability of the microscope to distinguish between two separate and very close objects so that they are distinct. It can also be defined as the degree to which the details of the objects are shown or a measure of clarity of the image. If two separate objects cannot be resolved, they will be seen as one object. The difference between the magnification and resolution of a microscope is that magnification can be changed (i.e increased or decreased) while resolution is always the same. The resolving power of the light microscope is always lower (200nm) compared to that of an electron microscope (0.5nm). This resolution is limited by the wave length of the visible light used to illuminate the object. the light microscope can magnify effectively to a bout 1000 times the of the actual specimen since greater magnification a above this increases blurredness. Advantages of a light microscope; It is cheaper and easier to operate when compared to the electron microscope. It can used to observe living specimens directly since it does not require a vacuum like the electron microscope. It does not result in the destruction of cellular organization like the electron microscope. Disadvantages of a light microscope; It has a low resolving power. It has a low magnifying power. Modifications of the light microscope. Type of microscope Mode of operation Main use Ultra violet microscope Uses quartz lenses Study chromosome changes during cell division Dark field microscope Uses light at right angles Study very small to its optical axis and particles about 8nm depends on the scattering of light by different particles. 3 Systematic “A” level Biology. Basic and simplified Revision notes. Phase contrast Depends on the Study transparent cell microscope refraction of light by parts e.g cytoplasm. parts the cell basin on their refractive indices. Polarizing microscope Uses polarized light. Study layer-like structures in the cell e.g spindle fibres, myosin filaments in stratified muscle, cellulose in cell wall etc. Electron Microscopes. In electron microscope a beam of electrons is used. Electrons are negatively charged particles that can be found in orbit around nucleus of atoms. Under certain circumstances they can behave as waves. They have two great advantages over light ie they have extremely short wave length, about the same as X- rays and the beam electrons can easily be focused through specimen using electro-magnets. This involves bending the beam of electrons just as glass lenses are used to bend light with electron microscope magnification have x250,000 are commonly obtained with biological materials. Resolution and magnification. Resolution is the ability to distinguish between 2 separate objects. Magnification can be increased but the resolution of the photograph stays the same. We enlarge the photographs in order to see them more clearly but if we go too far the picture bright less up in 2 separate blood dots. The resolution is much greater than electron- microscope, the shorter the wave lengths are said to greater resolving power than those of light. Structure of electron microscope. Advantages of electron microscope. High resolution (0 – 5nm in practice). It magnifies very tiny objects. 4 Systematic “A” level Biology. Basic and simplified Revision notes. Disadvantages of electron – microscope. The specimen must be dead because it is viewed in vacuum. It is difficult to be sure that the specimen resembling a living cell in all its details because preservation and staining may change or damage the structure. Expensive to buy and run. Preparation of material is time consuming and requires expert training. The specimen is gradually detoriates in the electron beam photographs must there fore be taken it future study is required. Comparison between a light and an electron microscope. (i)Similarities between a light and an electron microscope. In both, specimens are magnified to be seen clearly. In both, specimens to be observed are illuminated. Both have an eye piece where an observer views the specimen. Both have a condenser to control the amount of illuminating agent. Both have a stage. Both have lenses. (ii) Differences: Light microscope. Electron microscope. Uses light to illuminate the specimen. Uses electron to illuminate the specimen. Has low magnifying power (x1500). Has high magnifying power (x300, 000). Produces images in their natural Produces all images in black and colours. white. Small and portable. Very large and only operated in a special place. Not affected by magnetic field. Affected by magnetic field. Has low resolving power (200nm). Has high resolving power (0.5nm). Used to view living and non-living Used to view non – living specimens. specimens. The specimen is supported by a glass The specimens supported on a small slide. copper grid in a vacuum. Uses glass lenses. Uses electromagnetic coils as lenses. Operates in the wave length of about Operates in the wave length of about 400-700mm 0.005mm Types of electron microscope