Food Protein Analysis: Qualitative Effects on Processing
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Food Production and Nutrition AFOO-0000-O000 Developing Countries Proteins Food Supply
AGENCY FOR INTERNATIONAL DEVELOPMENT FOR AID USE 0 Y WASHINGTON, D. C. 20523 BIBLIOGRAPHIC INPUT SHEET A.P RIIAAft'0 1..AU.JECT Food production and nutrition AFOO-0000-O000 f.LASSI. FICATION f.31'~IA1 , Plant production 2. TITLE AND SUBTITLE Report 3. AUTHOR(S) (101) Workshop on Assaying Improved High Protein Crop Cultivars for Developing Countries.Washinqton.D.C. 1971; AID/TA/AGR 4. DOCUMENT DATE 5. NUMBER OF PAGES 6. ARC NUMBER 19711 30p. ARC 7. REFERENCE ORGANIZATION NAME AND ADDRESS AID/TA/AGR 6. SUPPLEMENTARY NOTES (Sponsoring Organization, Publishera, Availability) 9. ABSTRACT 10. CONTROL NUMBER !i. PRICE OF DOCUMENT 12. DESCRIPTORS 13. PROJECT NUMBER Assaying Meetings Developing countries Proteins 14. CONA Farm crops Food supply IS. TYPE OF DOCUMENT AID 590.1 (4.74) ASSAYING IMPROVED HIGH PROTEIN CROP CULTIVARS FOR DEVELOPING COUNTRIES Report of Workshop, Washington, D.C. May 6-7, 1971 Convened by Office of Agriculture and Fisheries, Technical Assistance Bureau U.S. Agency for International Development Washington, D.C. 20523 INTRODUCTION TA/AGF is engaged in sponsoring research investigations and training of responsible scientists in improving high protein crop cultivars and pro duction for developing nations. This effort is being supported directly from research being conducted in the United States with winter wheat at the University of Nebraska, with maize at Purdue University, with sorghum at Purdue and Puerto Rico Universities, with cassava at Georgia University, with soybeans at Illinois and Puerto Rico Universities, with beans and cowpeas at Puerto Rico (USDA cooperation) and to a lesser extent with yams and cassava at Puerto Rico. -
Agricultural Experiment Station News April 1977
University of Nebraska - Lincoln DigitalCommons@University of Nebraska - Lincoln Agricultural Research Division News & Annual Reports Agricultural Research Division of IANR 4-1977 Agricultural Experiment Station News April 1977 Follow this and additional works at: https://digitalcommons.unl.edu/ardnews Part of the Agriculture Commons "Agricultural Experiment Station News April 1977" (1977). Agricultural Research Division News & Annual Reports. 197. https://digitalcommons.unl.edu/ardnews/197 This Article is brought to you for free and open access by the Agricultural Research Division of IANR at DigitalCommons@University of Nebraska - Lincoln. It has been accepted for inclusion in Agricultural Research Division News & Annual Reports by an authorized administrator of DigitalCommons@University of Nebraska - Lincoln. •l~: ;\ n .. z .. .+".N_'O . rHE AGRICULTURAL EXPERIMENT STATION INSTITUTE OF AGRICULTURE AND NATURAL " RESOURCES UNIVERSITY OF NEBRASKA-LINCOLN H. W. OnOSON, DIRECTOR Vol. 9, No. 10, April 1977 FROM THE DIRECTOR'S DESK At no time during the past 15 years has there been more discussion at national levels about agricultural research. There are several reasons for this; international conferences on world population, and on world food, have raised questions about the ability of the world's food producers to meet future food needs and demands of this population. The convergenc~ of factors which led to the food supply-price situation of 1973-74 threw an additional scare into the minds of policy makers concerned with these issues. Admittedly, the apparent "crisis" has eased off for the moment with higher levels of stocks and lower prices, but the basic long-run concern about food production continues. There seems to be a consensus in these discussions that the most important factor contributing to long-term growth in agricultural productivity has been technological change. -
Hiper® Protein Estimation Teaching Kit (Quantitative)
U n z i p p i n g G e n e s P r o d u c t I n f o r m a t i o n HiPer® Protein Estimation Teaching Kit (Quantitative) Product Code: HTBC005 Number of experiments that can be performed: 5 Duration of Experiment Protocol Biuret assay: 1 hour Folin – Lowry Assay: 2 hours Bradford assay: 1 hour Storage Instructions: The kit is stable for 12 months from the date of manufacture Store all the kit contents as specified in the brochure 1 Registered Office : Commercial Office 23, Vadhani Industrial Estate,LBS Marg, A-516, Swastik Disha Business Park, Tel: 00-91-22-6147 1919 15 WHO Mumbai - 400 086, India. Via Vadhani Indl. Est., LBS Marg, Fax: 6147 1920, 2500 5764 GMP Tel. : (022) 4017 9797 / 2500 1607 Mumbai - 400 086, India Email : [email protected] CERTIFIED Fax : (022) 2500 2286 Web : www.himedialabs.com The information contained herein is believed to be accurate and complete. However no warranty or guarantee whatsoever is made or is to be implied with respect to such information or with respect to any product, method or apparatus referred to herein Index Sr. No. Contents Page No. 1 Aim 3 2 Introduction 3 3 Materials Required But Not Provided 3 4 Storage 3 5 Biuret Assay 3 Principle Kit contents Important Instructions Procedure Observation and Result Interpretation 6 Folin – Ciocalteau (Lowry) Assay 6 Principle Kit contents Important Instructions Procedure Observation and Result Interpretation 7 Dye Binding (Bradford) Assay 9 Principle Kit contents Important Instructions Procedure Observation and Result Interpretation 8 Troubleshooting Guide 12 2 Aim: To determine the concentration of a protein by three commonly used methods: 1. -
Illinois Vegetable Farmers' Letter
Digitized by the Internet Archive in 2011 with funding from University of Illinois Urbana-Champaign http://www.archive.org/details/illinoisvegetabl515univ NOTICE: Return or renew ail Library Materials! The Minimum Fee for each Lost Book is $50.00. The person charging this material is responsible for its return to the library from which it was withdrawn on or before the Latest Date stamped below. Theft, mutilation, and underlining of books are reasons for discipli- nary action and may result in dismissal from the University. To renew call Telephone Center, 333-8400 UNIVERSITY OF ILLINOIS LIBRARY AT URBANA-CHAMPAIGN L161—O-I096 JUN 6 2000 AGRICULTURE LIBRARY /o<z EXTENSION SERVICE Acx c - w COOPERATIVE 'C V3<P 5i COLLEGE OF AGRICULTURE J2 UNIVERSITY OF ILLINOIS flj AT URBANA — CHAMPAIGN b^ March 1985 \°$> \^^ NEWSLETTER SUPPORTERS The 1985 issue of the Illinois Vegetable Farmer's Letter is cur- rently being supported by the following companies: J.R. Kelly Co. Paarlberg Chemicals Cole Chemical Co. FMC - Agriculture Chemicals Group Harris Moran Seed Co. Potash Producers, Inc. We greatly appreciate the support from these companies. In the past, however, we have had almost 30 sponsors. If we do not re- ceive additional assistance, the newsletter will have to be dis- continued. please give us your support so that we may continue to be a primary source of information on commercial vegetable production. Other companies and organizations that would like to help sponsor the Illinois Vegetable Farmer's Letter, should contact John M. Gerber (217)333-1969. - U OF I ACTIVITIES ON COMMERCIAL VEGETABLE PRODUCTION 1984 This issue of the newsletter highlights some of the activities of the vegetable research and Extension staff during 1984. -
2019 KSFE Product Catalogue
Laboratory Chemicals 209 Description Brand Reference Packing Acacia powder, “Biochem”. R&M 2924-00 500g Acenaphthene, C.P. R&M 0003-00 500g ACES, “Biochem”. [N-(Acetomido)-2-aminoethanesulfonic acid] R&M 2340-00 25g Acetamide, C.P. (Ethanamide) R&M 0004-00 500g Acetanilide, A.R. (N-Phenylacetamide) R&M 0006-50 500g Acetate Buffer, for chlorine (pH-4), “READIL”. R&M 1560-00 500ml Acetate Buffer, pH-5.50, “READIL”. R&M 1560-55 500ml Acetic Acid, glacial, A.R. (Ethanoic acid) R&M 1410-58 2.5Lt Acetic Acid, 95%, A.R. (Ethanoic acid) R&M 1410-59 2.5Lt Acetic Acid, 5% (W/V), "READIL”. (Vinegar) R&M 1411-05 1Lt Acetic Acid, 10% (W/V), "READIL”. R&M 1411-10 1Lt Acetic Acid, 30% (W/V), "READIL”. R&M 1410-53 1Lt Acetic Acid, 50% (W/V), "READIL”. R&M 1410-50 1Lt Acetic Acid, 0.05mol/l (0.05), "READIL”. R&M 1410-51 1Lt Acetic Acid, 0.1mol/l (0.1), "READIL”. R&M 1410-52 1Lt Acetic Acid, 0.5mol/l (0.5), "READIL”. R&M 1410-53 1Lt Acetic Acid, 1.0mol/l (1.0), "READIL”. R&M 1410-54 1Lt Acetic Alcohol, “READIL”. R&M 1410-30 1Lt Acetoacetanilide, C.P. R&M 5298-50 500g Aceto-Carmine, “Biochem”. R&M 0063-80 100ml Aceto-Orcein, “Biochem”. (Connective tissue stain) R&M 0299-80 100ml Acetone, A.R. R&M 1412-50 2.5Lt Acetone, C.P. R&M 9108-50 2.5Lt Acetone, HPLC. R&M 9108-20 2.5Lt Acetone:Alcohol, “READIL”. -
Modified Lowry Protein Assay Kit
INSTRUCTIONS Modified Lowry Protein Assay Kit 0389.6 23240 Number Description 23240 Modified Lowry Protein Assay Kit, sufficient reagents for 480 test tubes or 2400 microplate assays Kit Contents: Modified Lowry Protein Assay Reagent, 480mL, containing cupric sulfate, potassium iodide, and sodium tartrate in an alkaline sodium carbonate buffer 2N Folin-Ciocalteu Reagent, 50mL Albumin Standard Ampules, 2mg/mL, 10 × 1mL ampules containing bovine serum albumin (BSA) at a concentration of 2.0mg/mL in 0.9% saline and 0.05% sodium azide; store at 4°C or room temperature Storage: Upon receipt store at 4°C. Product shipped at ambient temperature in two separate packages. IMPORTANT NOTE: To comply with Department of Transportation (DOT) shipping regulations, the 2N Folin-Ciocalteu Reagent is shipped in a separate package from the remaining components. Upon receipt of both packages, components may be placed together in a single kit box for storage. Table of Contents Introduction ................................................................................................................................................................................. 1 Preparation of Standards and Folin-Ciocalteu Reagent ............................................................................................................... 2 Test Tube Procedure .................................................................................................................................................................... 2 Microplate Procedure .................................................................................................................................................................. -
Thermo Scientific Pierce Protein Assay Technical Handbook Version 2
Thermo Scientific Pierce Protein Assay Technical Handbook Version 2 Table of Contents Total Protein Assays Specialty Assays Quick Technical Summaries 1 Histidine-tagged Proteins 32 Thermo Scientific HisProbe-HRP Kits 32 Introduction 4 Selection of the Protein Assay 4 Antibodies 33 Selection of a Protein Standard 5 IgG and IgM Assays 33 Standard Preparation 6 Proteases 35 Standards for Total Protein Assay 7 Compatible and Incompatible Substances 9 Protease Assays 35 Compatible Substances Table 10 Glycoproteins 36 Time Considerations 12 Glycoprotein Carbohydrate Estimation Assay 36 Calculation of Results 12 Phosphoproteins 37 Thermo Scientific Pierce 660nm Protein Assay 13 Phosphoprotein Phosphate Estimation Assay 37 Overview 13 Highlights 13 Peroxides 38 Typical Response Curves 14 Quantitative Peroxide Assay 38 BCA-based Protein Assays 15 Spectrophotometers 39 Chemistry of the BCA Protein Assay 15 BioMate 3S UV-Visible Spectrophotometer 39 Advantages of the BCA Protein Assay 16 Evolution 260 Bio UV-Visible Spectrophotometer 39 Disadvantages of the BCA Protein Assay 17 Evolution 300 UV-Visible Spectrophotometer 40 BCA Protein Assay – Reducing Agent Compatible 18 Evolution Array UV-Visible Spectrophotometer 40 BCA Protein Assay 19 Micro BCA Protein Assay 20 Coomassie Dye-based Protein Assays (Bradford Assays) 21 Chemistry of Coomassie-based Protein Assays 21 Advantages of Coomassie-based Protein Assays 21 Disadvantages of Coomassie-based Protein Assays 21 General Characteristics of Coomassie-based Protein Assays 22 Coomassie Plus (Bradford) -
Biochemical Aspects of Seeds from Cannabis Sativa L. Plants Grown In
www.nature.com/scientificreports OPEN Biochemical aspects of seeds from Cannabis sativa L. plants grown in a mountain environment Chiara Cattaneo1*, Annalisa Givonetti1, Valeria Leoni2,3, Nicoletta Guerrieri4, Marcello Manfredi5, Annamaria Giorgi2,3 & Maria Cavaletto1 Cannabis sativa L. (hemp) is a versatile plant which can adapt to various environmental conditions. Hempseeds provide high quality lipids, mainly represented by polyunsaturated acids, and highly digestible proteins rich of essential aminoacids. Hempseed composition can vary according to plant genotype, but other factors such as agronomic and climatic conditions can afect the presence of nutraceutic compounds. In this research, seeds from two cultivars of C. sativa (Futura 75 and Finola) grown in a mountain environment of the Italian Alps were analyzed. The main purpose of this study was to investigate changes in the protein profle of seeds obtained from such environments, using two methods (sequential and total proteins) for protein extraction and two analytical approaches SDS-PAGE and 2D-gel electrophoresis, followed by protein identifcation by mass spectrometry. The fatty acids profle and carotenoids content were also analysed. Mountain environments mainly afected fatty acid and protein profles of Finola seeds. These changes were not predictable by the sole comparison of certifed seeds from Futura 75 and Finola cultivars. The fatty acid profle confrmed a high PUFA content in both cultivars from mountain area, while protein analysis revealed a decrease in the protein content of Finola seeds from the experimental felds. Cannabis sativa L. (hemp) is an annual plant belonging to the family of Cannabaceae. C. sativa is naturally dioe- cious, however this plant has been domesticated by humans since the prehistoric era and monoecious varieties have been selected to obtain higher quality fbers and to optimize seed harvest procedures1. -
Lowry Protein Assay Kit KB-03-004 1200 Test (96 Well Plate)
Lowry Protein Assay Kit KB-03-004 1200 test (96 well plate) Index Introduction Pag. 1 Materials Pag. 2 Assay Principle Pag. 3 Reagent Preparation Pag. 6 Assay Protocol Pag. 7 Data Analysis Pag. 10 Warranties and Limitation of Liability Pag. 11 All chemicals should be handled with care ➢ ➢ This kit is for R&D use only Introduction Lowry Protein Quantification Assay is based on Lowry method, first described in 1951. The method relies on two different reactions. The first is the formation of a copper ion complex with amide bonds, forming reduced copper in alkaline solutions. This is called a Biuret chromophore and is commonly stabilized by the addition of tartrate. The second reaction is reduction of Folin-Ciocalteu reagent (phosphomolybdate and phosphotungstate), primarily by the reduced copper-amide bond complex as well as by tyrosine, tryptophan, histidine, cystine, and cysteine residues in protein.The monovalent copper ion catalyzes the latter reaction. The reduced Folin-Ciocalteu reagent is blue and thus detectable with a spectrophotometer in the range of 500 to 750 nm. The Biuret reaction itself is not very sensitive. Using the Folin-Ciocalteu reagent to detect reduced copper makes the Lowry assay nearly 100 times more sensitive than the Biuret reaction alone. 1 Materials BQCkit Lowry Protein Quantification Assay kit KB-03-004 1200 tests contains: Product Quantity Storage Lowry Reagent A 2 bottles RT Lowry Reagent B 1 vial RT Lowry Reagent C 1 bottle RT Protein Standard* 5 vials 4ºC *This reagent is stable during 10 days at Room Temperature and is shipped in these conditions. -
Modified Lowry Protein Assay Reagent Kit, Sufficient Reagents for 480 Test Tube Or 2,400 Microplate Assays
INSTRUCTIONS Modified Lowry Protein 3747 N. Meridian Road P.O. Box 117 Assay Reagent Kit Rockford, IL 61105 23240 0389w Number Description 23240 Modified Lowry Protein Assay Reagent Kit, sufficient reagents for 480 test tube or 2,400 microplate assays Kit Contents: Modified Lowry Protein Assay Reagent, 480 ml, containing cupric sulfate, potassium iodide, and sodium tartrate in an alkaline sodium carbonate buffer. 2N Folin-Ciocalteu Reagent, 50 ml Albumin Standard Ampules, 2 mg/ml, 10 x 1 ml ampules containing bovine serum albumin (BSA) at a concentration of 2.0 mg/ml in 0.9% saline and 0.05% sodium azide Storage: Upon arrival store at 4°C. Product shipped at ambient temperature. Note: Discard any kit reagent that shows discoloration or evidence of microbial contamination. This product is guaranteed for one year from the date of purchase when handled and stored properly. Table of Contents Introduction..................................................................................................................................................................................1 Preparation of Standards and Folin-Ciocalteu Reagent ...............................................................................................................2 Table 1: Preparation of Diluted Albumin (BSA) Standards ........................................................................................................2 Procedure Summary (Test Tube Procedure)................................................................................................................................2 -
Protein Quantification and Qualification Application Compendium Protein Quantification Using the Nanodrop One Spectrophotometer
Protein quantification and qualification Application compendium Protein quantification using the NanoDrop One Spectrophotometer Welcome to protein quantification and qualification e-book. Here you’ll find a compendium of useful technical documents, application notes, and protocols for measuring proteins using the Thermo Scientific™ NanoDrop™ One/OneC Microvolume UV-Vis Spectrophotometer. Scientists can use the NanoDrop One/OneC instrument to quantify the protein content in their sample using either direct or indirect measurements. An example of a direct measurement is the Protein A280 application, which calculates protein concentration based on the sample absorbance at 280 nm and the protein- and wavelength-specific extinction coefficient. Colorimetric assays are an example of an indirect measurement. The NanoDrop One/OneC Software is hardcoded with applications to measure the product of the BCA, Bradford, Lowry, and Pierce™ 660 nm reactions. Feel free to contact us at [email protected] if you have any protein quantification or qualification questions. Table of contents Blanking with high absorbing buffers such as RIPA negatively affects Protein A280 measurements ................ 3 NanoDrop One educational animations ............................................................. 6 Quantify protein and peptide preparations at 205 nm................................................... 7 On-demand webinar: Protein Sample Evaluation using the NanoDrop One UV-Vis Spectrophotometer .............10 BCA protein assay .............................................................................11 -
No. 4 DECEMBER 1967
VI CONTENTS Transmural Movements of Zinc, Manganese, Cadmium and Mercury by Rat Small Intestine. Benjamin M. Sahagian, I. Harding-Barlow and H. Mitchell Perry, Jr 291 Alterations in the Activities of Several Rat Liver Enzymes at Various Times after Initiation of a High Protein Regimen. B. Szepesi and R. A. Freed/and 301 Interrelationships among Copper, Zinc, and Cadmium in the Diet of the Confused Flour Beetle. John C. Medici and M. Wight Taylor 307 Effect of Isoniazid on the Urinary Excretion of Taurine by Men. Frances A. Johnston, Elizabeth A. Donald and Nina H. Mercer 310 Some Effects of Amino Acids and Cortisol on Tyrosine Toxicity in the Rat. Downloaded from https://academic.oup.com/jn/issue/93/4 by guest on 27 September 2021 S. Q. A/am, A. M. Boctor, Q. R. Rogers and A. E. Harper 317 Absorption and Metabolism of Dietary Triglycérides in Germfree and Conventional Rats. Norbert L. Wiech, James G. Hamilton and O. Neal Miller 324 Trace Metals in Rat Tissues as Influenced by Calcium in Water. Henry A. Schroeder, Alexis P. Nason and Joseph J. Balassa 331 Long-term Rat Feeding Study with Used Frying Fats. Granville A. Nolen, J. Craig Alexander and Neil R. Artman 337 Protein Quality of a Soybean Protein Textured Food in Experimental Animals and Children. Ricardo Bressani, Fernando Viteri, Luiz G. Elias, Silvia de Zaghi, Jorge Alvarado and A. D. Odell 349 Effects of Thyroid Hormone and Phosphorus Loading on Renal Calcifica tion and Mineral Metabolism of the Rat. D. L. Meyer and R. M. Forbes 361 Utilization of Amino Acids from Protein by Weanling Pigs.