DOCSLIB.ORG

Pharmaceutical and Veterinary Compounds and Metabolites

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Pharmaceutical and Veterinary Compounds and Metabolites PHARMACEUTICAL AND VETERINARY COMPOUNDS AND METABOLITES High quality reference materials for analytical testing of pharmaceutical and veterinary compounds and metabolites. lgcstandards.com/drehrenstorfer [email protected] LGC Quality | ISO 17034 | ISO/IEC 17025 | ISO 9001 PHARMACEUTICAL AND VETERINARY COMPOUNDS AND METABOLITES What you need to know Pharmaceutical and veterinary medicines are essential for To facilitate the fair trade of food, and to ensure a consistent human and animal welfare, but their use can leave residues in and evidence-based approach to consumer protection both the food chain and the environment. In a 2019 survey of across the globe, the Codex Alimentarius Commission (“Codex”) EU member states, the European Food Safety Authority (EFSA) was established in 1963. Codex is a joint agency of the FAO found that the number one food safety concern was the (Food and Agriculture Office of the United Nations) and the misuse of antibiotics, hormones and steroids in farm animals. WHO (World Health Organisation). It is responsible for producing This is, in part, related to the issue of growing antibiotic and maintaining the Codex Alimentarius: a compendium of resistance in humans as a result of their potential overuse in standards, guidelines and codes of practice relating to food animals. This level of concern and increasing awareness of safety. The legal framework for the authorisation, distribution the risks associated with veterinary residues entering the food and control of Veterinary Medicinal Products (VMPs) varies chain has led to many regulatory bodies increasing surveillance from country to country, but certain common principles activities for pharmaceutical and veterinary residues in food apply which are described in the Codex guidelines. and water supplies. For example, the EU Directive 96/23/EC sets out the requirements for residue monitoring plans and establishes On the following pages you will the range of substances to be tested in live animals and animal products. In addition, each EU member state must find an extensive selection of our have a National Residue Control Plan (NRCP). NRCPs are most popular pharmaceutical and targeted surveillance testing designed to police the correct veterinary reference materials, use of veterinary medicinal products. Non-EU countries exporting food to the EU must implement monitoring including where available the programmes which comply with equivalent food safety levels. parent compounds, associated metabolites and Stable Isotope In China, the Ministry of Agriculture Announcement No. 235 (中华人民共和国农业部公告第235号) sets out the requirements Labelled (SIL) compounds. for residue monitoring plans and establishes the range of substances to be tested in live animals and animal products. The government also sets National Food Safety Inspections Visit lgcstandards.com to explore (国家食品安全监督抽检) every year to police the correct use the full and extensive range of over of veterinary medicinal products to ensure food safety. In 8,000 Dr. Ehrenstorfer reference addition, a new GB method is currently in development, entitled ‘National food safety standard - Maximum Residue materials available for food and Limits For Veterinary Drugs In Animal Derived Food’ (‘食品安 environmental analysis. 全国家标准 动物性食品中兽药最大残留限量 (报批稿)’). 2 Discover our pharmaceutical and veterinary compounds and metabolites range: lgcstandards.com/drehrenstorfer Our Dr. Ehrenstorfer range of pharmaceutical and veterinary We are continually adding products to this portfolio to reference materials has been designed to support you in maintain pace with emerging and evolving analytical your analytical testing and address different regulatory methods requirements. Our latest and full range can always be applied around the world. You will find our comprehensive explored on our website lgcstandards.com. offering includes both metabolites and stable isotope labelled (SIL) compounds for many of our parent compounds. With all measurements on our certificate of analysis carried Testing the active drug is often not sufficient, since they out in accordance with ISO 17025 and a majority of products are metabolized into other biologically active substances. produced under the scope of our ISO 17034 accreditation, As more research is conducted in this area, metabolites we guarantee the highest quality reference materials for your are increasingly being written into regulations with specific analytical testing. maximum residue limits, necessitating metabolite testing. SIL compounds can be added to samples to negate the ‘matrix effects’ often observed with mass spectrometric techniques such as LC-MSMS, further ensuring the reliability of your analysis. If you’re looking for pre-prepared solutions and mixtures but can’t find what you need, we can help. Dr. Ehrenstorfer Custom-Made solutions provide cost effective and precise options that are tailored to you. Visit lgcstandards.com to learn more. 3 Discover our pharmaceutical and veterinary compounds and metabolites range: lgcstandards.com/drehrenstorfer pharmaceutical and veterinary compounds and metabolites Dr. Ehrenstorfer has an extensive range of neat, single solution and multicomponent solution products for the analytical testing of pharmaceutical and veterinary compounds and metabolites. In addition, we offer not just the parent compounds, but also Stable Isotope Labelled (SIL) compounds, associated metabolites and SIL variants of metabolites for some products. On the following pages you will find an index of selected parent compounds and available associated SILs and metabolites, allowing you to thoroughly test for pharmaceutical and veterinary compounds. You can also visit lgcstandards.com/drehrenstorfer to browse the full range of products. Product code Description Pack size CAS number SIL Metabolite SIL of Metabolite DRE-C10008000 Acenocoumarol 10mg 152-72-7 NEW DRE-C10010300 Acepromazine maleate 100mg 3598-37-6 DRE-C10010320 Acepromazine D6 maleate 10mg 1331655-50-5 • DRE-C10016950 Acetildenafil 10mg 831217-01-7 DRE-C10024000 Acetylsalicylic acid 250mg 50-78-2 DRE-C10024050 Acetylsulfamethoxazole 100mg 21312-10-7 DRE-C10024051 Acetylsulfamethoxazole D4 10mg 1215530-54-3 • DRE-C10045820 Adenosine 250mg 58-61-7 DRE-C10065000 Albendazole 100mg 54965-21-8 DRE-C10065020 Albendazole-2-amino 100mg 80983-36-4 • DRE-C10065200 Albendazole-2-aminosulfone 50mg 80983-34-2 • DRE-C10065250 Albendazole-2-aminosulfoxide 50mg 80983-35-3 • DRE-C10065300 Albendazole-sulfone 10mg 75184-71-3 • DRE-C10065400 Albendazole-sulfoxide 10mg 54029-12-8 • DRE-C10065410 Albendazole-sulfoxide D3 (methyl D3) 10mg 1448346-38-0 • NEW DRE-C10098000 Allantoin 100mg 97-59-6 NEW DRE-C10144000 Altrenogest 100mg 850-52-2 DRE-C10146000 Amantadine hydrochloride 100mg 665-66-7 DRE-C10164000 Amikacin hydrate 100mg 1257517-67-1 DRE-C10185000 Aminocaproic acid 100mg 60-32-2 NEW DRE-C10242500 Amoxicillin trihydrate 250mg 61336-70-7 DRE-C10243080 Ampicillin trihydrate 100mg 7177-48-2 DRE-C10255010 5alpha-Androstan-17beta-ol-3-one 100mg 521-18-6 DRE-C10258000 Anhydrotetracycline hydrochloride 10mg 13803-65-1 NEW DRE-C10293000 Apramycin sulfate 250mg 65710-07-8 DRE-C10300190 L-Arginine 100mg 74-79-3 NEW * Indicates where a parent compound has a subproduct 4 Discover our pharmaceutical and veterinary compounds and metabolites range: lgcstandards.com/drehrenstorfer Product code Description Pack size CAS number SIL Metabolite SIL of Metabolite DRE-C10300550 Artesunate 100mg 88495-63-0 NEW DRE-C10304960 L-Aspartic acid 100mg 56-84-8 NEW DRE-C10313000 Atenolol 100mg 29122-68-7 DRE-C10333500 Atropine 250mg 51-55-8 DRE-C10340500 Azaperol 10mg 2804-05-9 DRE-C10340510 Azaperone 50mg 1649-18-9 DRE-C10340512 Azaperone D4 10mg 1173021-72-1 • DRE-C10385990 Azithromycin 25mg 83905-01-5 NEW DRE-C10386000 Azithromycin dihydrate 50mg 117772-70-0 DRE-C10418000 Bacitracin 250mg 1405-87-4 DRE-C10418500 Bambuterol hydrochloride 10mg 81732-46-9 DRE-C10532490 Benzathine penicilline G 100mg 1538-09-6 NEW DRE-C10532500 Benzathine penicilline G tetrahydrate 100mg 41372-02-5 DRE-C10573950 Berberine chloride 100mg 633-65-8 NEW DRE-C10574920 Betaine hydrochloride 50mg 590-46-5 NEW DRE-C10575000 Betamethasone 100mg 378-44-9 DRE-C10578000 Bezafibrate 100mg 41859-67-0 DRE-C10578010 Bezafibrate D6 (dimethyl D6) 10mg 1219802-74-0 • DRE-C10653900 Bisoctrizole 100mg 103597-45-1 DRE-C10662000 Boldenone 10mg 846-48-0 DRE-C10662200 Boldenone undecylenate 10mg 13103-34-9 NEW DRE-C10683000 Brombuterol hydrochloride 10mg 21912-49-2 DRE-C10844500 Buparvaquone 25mg 88426-33-9 NEW DRE-C10933500 Cadaverine dihydrochloride 100mg 1476-39-7 DRE-C11693000 Caffeine 250mg 58-08-2 DRE-C10962000 Captopril 250mg 62571-86-2 DRE-C10968000 Carazolol 10mg 57775-29-8 DRE-C10968010 Carazolol D7 10mg 1173021-02-7 • DRE-C10968300 Carbadox 100mg 6804-07-5 DRE-C16713000 2-Quinoxalinecarboxylic acid 100mg 879-65-2 • DRE-C16713001 2-Quinoxalinecarboxylic acid D4 10mg • NEW (5,6,7,8 D4) DRE-C10968500 Carbamazepin 100mg 298-46-4 DRE-C11045500 L-Carnitin 100mg 541-15-1 DRE-C11064000 Cefalexin monohydrate 250mg 23325-78-2 DRE-C11064100 Cefazolin sodium 100mg 27164-46-1 DRE-C11064300 Cefoperazone sodium 100mg 62893-20-3 DRE-C11064700 Cefquinome sulfate 100mg 118443-89-3 5 Discover our pharmaceutical and veterinary compounds and metabolites range: lgcstandards.com/drehrenstorfer Product code Description Pack size CAS number SIL Metabolite SIL of Metabolite DRE-C11064800 Cefradine 100mg 38821-53-3 DRE-C11065000 Ceftiofur 100mg 80370-57-6 DRE-C11065300 Cefuroxime sodium 100mg 56238-63-2 DRE-C11069000
Load more

Recommended publications
  • Scopoletin 8-Hydroxylase: a Novel Enzyme Involved in Coumarin
    bioRxiv preprint doi: https://doi.org/10.1101/197806; this version posted October 4, 2017. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 Scopoletin 8-hydroxylase: a novel enzyme involved in coumarin biosynthesis and iron- 2 deficiency responses in Arabidopsis 3 4 Running title: At3g12900 encodes a scopoletin 8-hydroxylase 5 6 Joanna Siwinska1, Kinga Wcisla1, Alexandre Olry2,3, Jeremy Grosjean2,3, Alain Hehn2,3, 7 Frederic Bourgaud2,3, Andrew A. Meharg4, Manus Carey4, Ewa Lojkowska1, Anna 8 Ihnatowicz1,* 9 10 1Intercollegiate Faculty of Biotechnology of University of Gdansk and Medical University of 11 Gdansk, Abrahama 58, 80-307 Gdansk, Poland 12 2Université de Lorraine, UMR 1121 Laboratoire Agronomie et Environnement Nancy- 13 Colmar, 2 avenue de la forêt de Haye 54518 Vandœuvre-lès-Nancy, France; 3INRA, UMR 14 1121 Laboratoire Agronomie et Environnement Nancy-Colmar, 2 avenue de la forêt de Haye 15 54518 Vandœuvre-lès-Nancy, France; 16 4Institute for Global Food Security, Queen’s University Belfast, David Keir Building, Malone 17 Road, Belfast, UK; 18 19 [email protected] 20 [email protected] 21 [email protected] 22 [email protected] 23 [email protected] 24 [email protected] 25 [email protected] 26 [email protected] 27 [email protected] 28 *Correspondence: [email protected], +48 58 523 63 30 29 30 The date of submission: 02.10.2017 31 The number of figures: 9 (Fig.
    [Show full text]
  • Morning Glory Systemically Accumulates Scopoletin And
    Morning Glory Systemically Accumulates Scopoletin and Scopolin after Interaction with Fusarium oxysporum Bun-ichi Shimizua,b,*, Hisashi Miyagawaa, Tamio Uenoa, Kanzo Sakatab, Ken Watanabec, and Kei Ogawad a Graduate School of Agriculture, Kyoto University, Kyoto 606Ð8502, Japan b Institute for Chemical Research, Kyoto University, Uji 611-0011, Japan. Fax: +81-774-38-3229. E-mail: [email protected] c Ibaraki Agricultural Center, Ibaraki 311Ð4203, Japan d Kyushu National Agricultural Experiment Station, Kumamoto 861Ð1192, Japan * Author for correspondence and reprint requests Z. Naturforsch. 60c,83Ð90 (2005); received September 14/October 29, 2004 An isolate of non-pathogenic Fusarium, Fusarium oxysporum 101-2 (NPF), induces resis- tance in the cuttings of morning glory against Fusarium wilt caused by F. oxysporum f. sp. batatas O-17 (PF). The effect of NPF on phenylpropanoid metabolism in morning glory cuttings was studied. It was found that morning glory tissues responded to treatment with NPF bud-cell suspension (108 bud-cells/ml) with the activation of phenylalanine ammonia- lyase (PAL). PAL activity was induced faster and greater in the NPF-treated cuttings com- pared to cuttings of a distilled water control. High performance liquid chromatography analy- sis of the extract from tissues of morning glory cuttings after NPF treatment showed a quicker induction of scopoletin and scopolin synthesis than that seen in the control cuttings. PF also the induced synthesis of these compounds at 105 bud-cells/ml, but inhibited it at 108 bud- cells/ml. Possibly PF produced constituent(s) that elicited the inhibitory effect on induction of the resistance reaction.
    [Show full text]
  • Part IV: Basic Considerations of the Psoralens
    CORE Metadata, citation and similar papers at core.ac.uk Provided by Elsevier - Publisher Connector THE CHEMISTRY OF THE PSORALENS* W. L. FOWLKS, Ph.D. The psoralens belong to a group of compoundsfled since biological activity of the psoralens and which have been considered as derivatives ofangelicins has been demonstrated. They appear coumarin, the furocoumarins. There are twelveto have specific biochemical properties which different ways a furan ring can be condensed withmay contribute to the survival of certain plant the coumarin molecule and each of the resultingspecies. Specifically these compounds belong to compounds could be the parent for a family ofthat group of substances which can inhibit certain derivatives. Examples of most of these possibleplant growth without otherwise harming the furocoumarins have been synthesized; but natureplant (2, 3, 5). is more conservative so that all of the naturally It is interesting that it was this property which occurring furocoumarins so far described turnled to the isolation of the only new naturally out to be derivativesof psoralen I or angelicinoccuring furocoumarin discovered in the United II (1). States. Bennett and Bonner (2) isolated tharn- nosmin from leaves of the Desert Rue (Tham- n.osma montana) because a crude extract of this 0 0 plant was the best growth inhibitor found among /O\/8/O\/ the extracts of a number of desert plants sur- i' Ii veyed for this property, although all the extracts showed seedling growth inhibition. One could I II speculate as to the role such growth inhibition plays in the economy of those desert plants These natural derivatives of psoralen and angeli-when survival may depend upon a successful cm have one or more of the following substituentsfight for the little available water.
    [Show full text]
  • Pharmaceutical and Veterinary Compounds and Metabolites
    PHARMACEUTICAL AND VETERINARY COMPOUNDS AND METABOLITES High quality reference materials for analytical testing of pharmaceutical and veterinary compounds and metabolites. lgcstandards.com/drehrenstorfer [email protected] LGC Quality | ISO 17034 | ISO/IEC 17025 | ISO 9001 PHARMACEUTICAL AND VETERINARY COMPOUNDS AND METABOLITES What you need to know Pharmaceutical and veterinary medicines are essential for To facilitate the fair trade of food, and to ensure a consistent human and animal welfare, but their use can leave residues and evidence-based approach to consumer protection across in both the food chain and the environment. In a 2019 survey the globe, the Codex Alimentarius Commission (“Codex”) was of EU member states, the European Food Safety Authority established in 1963. Codex is a joint agency of the FAO (Food (EFSA) found that the number one food safety concern was and Agriculture Office of the United Nations) and the WHO the misuse of antibiotics, hormones and steroids in farm (World Health Organisation). It is responsible for producing animals. This is, in part, related to the issue of growing antibiotic and maintaining the Codex Alimentarius: a compendium of resistance in humans as a result of their potential overuse in standards, guidelines and codes of practice relating to food animals. This level of concern and increasing awareness of safety. The legal framework for the authorisation, distribution the risks associated with veterinary residues entering the food and control of Veterinary Medicinal Products (VMPs) varies chain has led to many regulatory bodies increasing surveillance from country to country, but certain common principles activities for pharmaceutical and veterinary residues in food and apply which are described in the Codex guidelines.
    [Show full text]
  • Scopoletin 8-Hydroxylase a Novel Enzyme Involved in Coumarin Biosynthesis and Iron-Deficiency Responses in Arabidopsis
    Scopoletin 8-hydroxylase a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis Siwinska, J., Siatkowska, K., Olry, A., Grosjean, J., Hehn, A., Bourgaud, F., Meharg, A. A., Carey, M., Lojkowska, E., & Ihnatowicz, A. (2018). Scopoletin 8-hydroxylase a novel enzyme involved in coumarin biosynthesis and iron-deficiency responses in Arabidopsis. Journal of experimental botany, 69(7), 1735-1748. https://doi.org/10.1093/jxb/ery005 Published in: Journal of experimental botany Document Version: Publisher's PDF, also known as Version of record Queen's University Belfast - Research Portal: Link to publication record in Queen's University Belfast Research Portal Publisher rights Copyright the authors 2018. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. General rights Copyright for the publications made accessible via the Queen's University Belfast Research Portal is retained by the author(s) and / or other copyright owners and it is a condition of accessing these publications that users recognise and abide by the legal requirements associated with these rights. Take down policy The Research Portal is Queen's institutional repository that provides access to Queen's research output. Every effort has been made to ensure that content in the Research Portal does not infringe any person's rights, or applicable UK laws. If you discover content in the Research Portal that you believe breaches copyright or violates any law, please contact [email protected].
    [Show full text]
  • 69-17821 EINHELLIG, Frank Arnold, 1938- EFFECTS OF
    This dissertation has been microfihned exactly as received _ 69-17,821 EINHELLIG, Frank Arnold, 1938- EFFECTS OF SCOPOLETIN ON GROWTH, 00» EXCHANGE RATES, AND CONCENTRATION OF SCOPOLETIN, SCOPOLIN, AND CHLOROGENIC ACIDS IN TOBACCO, SUNFLOWER, AND PIGWEED. University Microfilms, Inc., Ann Arbor, Michigan This dissertation has been microfilmed exactly as received 69-17,821 EINHELLIG, Frank Arnold, 1938- The University of Oklahoma, Ph.D., 1969 Botany University Microfilms, Inc., Ann Arbor, Michigan THE UNIVERSITY OF OKLAHOMA GRADUATE COLLEGE EFFECTS OF SCOPOLETIN ON GROWTH, CO EXCHANGE RATES, AND CONCENTRATION OF SCOPOLETIN, SCOPOLIN, AND CHLOROGENIC ACIDS IN TOBACCO, SUNFLOWER, AND PIGWEED A DISSERTATION SUBMITTED TO THE GRADUATE FACULTY in partial fulfillment of the requirements for the degree of DOCTOR OF PHILOSOPHY BY FRANK ARNOLD EINHELLIG Norman, Oklahoma 1969 EFFECTS OF SCOPOLETIN ON GROWTH, CO EXCHANGE RATES, AND CONCENTRATION OF SCOPOLETIN, SCOPOLIN, AND CHLOROGENIC ACIDS IN TOBACCO, SUNFLOWER, AND PIGWEED APPROVED BY Jh. ,N- //■ // ^ '-S' DISSERTATION COMMITTEE ACKNOWLEDGMENT I wish to express my sincere thanks to my advisors, Dr. Elroy L. Rice and Dr. Paul G. Hisser, for their advice and encouragement during the course of this research. I am also very appreciative of the initial encouragement and help I received from Dr. Lawrence M. Rohrbaugh. The sug­ gestions, advice, and critical reading of the other mem­ bers of my committee, Dr. Simon H„ Wender and Dr. George Goodman, were also a tremendous aid in this work, I wish to express my thanks to my wife, Trude, and our children, Robert and Richard, for their understanding and sacrifice in the completion of this research.
    [Show full text]
  • Methyltransferases from Ruta Graveolens L.: Molecular Biology
    Methyltransferases from Ruta graveolens L.: Molecular Biology and Biochemistry Dissertation zur Erlangung des Doktorgrades der Naturwissenschaften (Dr. rer. nat.) dem Fachbereich Pharmazie der Philipps-Universität Marburg vorgelegt von Laura Nicoleta Burga aus Fagaras, Rumänien Marburg/Lahn 2005 Vom Fachbereich Pharmazie der Philipps-Universität Marburg als Dissertation angenomen am: 13. 07. 2005 Erstgutachter: Prof. Dr. Ulrich Matern Zweitgutachter: Prof. Dr. Maike Petersen Tag der mündlische Prüfung am: 12. 07. 2005 Publication Laura Burga, Frank Wellmann, Richard Lukacin, Simone Witte, Wilfried Schwab, Joachim Schröder and Ulrich Matern (2005) Unusual pseudosubstrate specificity of a novel 3,5-dimethoxyphenol O-methyltransferase cloned from Ruta graveolens L. Arch Biochem Biophys 2005 Aug 1; 440(1):54-64. _____________________________________________________________________ Table of contents ___ Table of contents i Abreviations vi 1. INTRODUCTION 1.1 Ruta graveolens L. 1 1.1.1 General features 1 1.1.2 Content 2 1.2 Acridone alkaloids 3 1.2.1 Biosynthesis of acridone alkaloids 4 1.2.2 Anthranilate synthase 5 1.2.3 Anthranilate N-methyltransferase 6 1.2.4 N-methylanthranilate:CoA-ligase 6 1.2.5 Acridone synthase 7 1.2.6 Rutacridone and furacridone 7 1.3 Methyltransferases 8 1.3.1 Plant methyltransferases 8 1.3.2 Conserved motifs 15 1.3.3 Active site 16 1.3.4 Importance of methyltransferases in humans 19 1.4 Plant volatile compounds 23 1.4.1 Methyltransferases involved in methylation of volatile compounds 24 1.4.2 Volatile compounds in
    [Show full text]
  • Effect of Scopoletin on Apoptosis and Cell Cycle Arrest in Human Prostate Cancer Cells in Vitro
    Li et al Tropical Journal of Pharmaceutical Research April 2015; 14 (4): 611-617 ISSN: 1596-5996 (print); 1596-9827 (electronic) © Pharmacotherapy Group, Faculty of Pharmacy, University of Benin, Benin City, 300001 Nigeria. All rights reserved. Available online at http://www.tjpr.org http://dx.doi.org/10.4314/tjpr.v14i4.8 Original Research Article Effect of Scopoletin on Apoptosis and Cell Cycle Arrest in Human Prostate Cancer Cells In vitro Chun-Long Li*, Xian-Cheng Han, Hong Zhang, Jin-Sheng Wu and Bao Li Department of Urology, Affiliated Hospital of Weifang Medical University, Weifang 261031, China *For correspondence: Email: [email protected]; Tel/Fax: 0086-536-8068850 Received: 5 November 2014 Revised accepted: 14 March 2015 Abstract Purpose: To investigate the anticancer activity of scopoletin against human prostate cancer. Methods: The anticancer activity of scopoletin was evaluated by 3-(4, 5-dimethylthiazol-2-yl)-2, 5- diphenyltetrazolium bromide (MMT) assay. Flow cytometry using propidium iodide and annexin V-FITC was employed to study apoptosis and cell cycle analysis. Hoechst 33258 staining was used to assess the effect of scopoletin on cell morphology and apoptotic body formation in human prostate carcinoma (LNCaP) cells via Florescence microscopy and finally Western blotting was used to evaluate the effect of scopoletin on cyclin D1 and cyclin B1 expressions. Results: Scopoletin induced a dose-dependent growth inhibition in LNCaP prostate cancer cells. It induced G2/M phase growth arrest and led to an increase in the sub-G0/G1 cell population after treatment with increasing doses compared to control cells, scopoletin treatment resulted in cell shrinkage along with membrane blebbing which are characteristic features of cell apoptosis.
    [Show full text]
  • Supporting Documents
    Supporting documents A single standard to determine multi-components method coupled with chemometric methods for the quantification, evaluation and classification of Notopterygii Rhizoma et Radix from different regions Xie-an Yu1,2, Jin Li1,†, John Teye Azietaku1,2, Wei Liu1,2, Jun He1,2*, Yan-xu Chang1,2* 1Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China 2Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China *Correspondence: [email protected] (J.H.); [email protected] (Y.C.); † These authors contributed equally to this work. Tel./Fax: + 86-22-59596163 (J.H. &Y.C.) Table1. Data for the calibration curves, LODs, LOQs, Rf and repeatability using ES method (n = 6). Line range LOD LOQ Repeatability Compounds Regressive equation -1 R Rf -1 -1 (µg mL ) (µg mL ) (µg mL ) RSD(%) Chlorogenic acid Y=8509.311X-113.31 0.2-50 0.9999 1 0.03 0.1 1.25 p-Coumaric acid Y=7248.429X-293.686 0.2-25 0.9996 0.783 0.06 0.2 2.39 Scopoletin Y=9064.661X+291.485 0.2-25 0.9998 2.234 0.06 0.2 1.81 Ferulic acid Y=11890.98X-1847.27 0.4-100 0.9999 1.291 0.06 0.2 2.36 Coumarin Y=2289.637X+10.744 0.4-50 0.9999 0.528 0.15 0.4 2.69 Nodakenin Y=8779.59X-291.147 0.4-100 0.9999 1.028 0.035 0.1 1.89 Bergaptol Y=5205.188X-195.825 0.2-50 0.9999 1.141 0.06 0.2 2.16 Psoralen Y=5343.163X-92.878 0.16-20 0.9999 1.163 0.05 0.15 4.09 Angelicin Y=2935.177X-2.095 0.16-20 0.9997 0.672 0.05 0.15 3.06 8-Methoxypsoralen
    [Show full text]
  • Pharmaceutical and Veterinary Compounds and Metabolites
    PHARMACEUTICAL AND VETERINARY COMPOUNDS AND METABOLITES High quality reference materials for analytical testing of pharmaceutical and veterinary compounds and metabolites. lgcstandards.com/drehrenstorfer [email protected] LGC Quality | ISO 17034 | ISO/IEC 17025 | ISO 9001 PHARMACEUTICAL AND VETERINARY COMPOUNDS AND METABOLITES What you need to know Pharmaceutical and veterinary medicines are essential for To facilitate the fair trade of food, and to ensure a consistent human and animal welfare, but their use can leave residues and evidence-based approach to consumer protection across in both the food chain and the environment. In a 2019 survey the globe, the Codex Alimentarius Commission (“Codex”) was of EU member states, the European Food Safety Authority established in 1963. Codex is a joint agency of the FAO (Food (EFSA) found that the number one food safety concern was and Agriculture Office of the United Nations) and the WHO the misuse of antibiotics, hormones and steroids in farm (World Health Organisation). It is responsible for producing animals. This is, in part, related to the issue of growing antibiotic and maintaining the Codex Alimentarius: a compendium of resistance in humans as a result of their potential overuse in standards, guidelines and codes of practice relating to food animals. This level of concern and increasing awareness of safety. The legal framework for the authorisation, distribution the risks associated with veterinary residues entering the food and control of Veterinary Medicinal Products (VMPs) varies chain has led to many regulatory bodies increasing surveillance from country to country, but certain common principles activities for pharmaceutical and veterinary residues in food and apply which are described in the Codex guidelines.
    [Show full text]
  • A Single Standard to Determine Multi-Components
    molecules Article A Single Standard to Determine Multi-Components Method Coupled with Chemometric Methods for the Quantification, Evaluation and Classification of Notopterygii Rhizoma et Radix from Different Regions 1,2, 1, 1,2 1,2 1,2, Xie-An Yu y, Jin Li y, John Teye Azietaku , Wei Liu , Jun He * and Yan-Xu Chang 1,2,* 1 Tianjin State Key Laboratory of Modern Chinese Medicine, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China; [email protected] (X.-A.Y.); [email protected] (J.L.); [email protected] (J.T.A.); [email protected] (W.L.) 2 Tianjin Key Laboratory of Phytochemistry and Pharmaceutical Analysis, Tianjin University of Traditional Chinese Medicine, Tianjin 300193, China * Correspondence: [email protected] (J.H.); [email protected] (Y.-X.C.); Tel./Fax: +86-22-59596163 (J.H. & Y.-X.C.) These authors contributed equally to this work. y Received: 1 September 2019; Accepted: 1 October 2019; Published: 3 October 2019 Abstract: An ultra-high-performance liquid chromatography-quadrupole/time of flight mass spectrometry is used to identify 33 compounds in Notopterygii rhizoma and radix, after which a single standard to determine multi-components method is established for the simultaneous determination of 19 compounds in Notopterygii rhizoma and radix using chlorogenic acid and notopterol as the internal standard. To screen the potential chemical markers among Notopterygii rhizoma and radix planted in its natural germination area and in others, the quantitative data of 19 compounds are analyzed via partial least-squares discriminant analysis (PLS–DA). Depending on the variable importance parameters (VIP) value of PLS–DA, six compounds are selected to be the potential chemical markers for the discrimination of Notopterygii rhizoma and radix planted in the different regions.
    [Show full text]
  • Ethnobotany of Rue (Ruta Graveolens L.): an Overview
    Delpinoa, n.s. 37-38: 63-72. 1995-1996 Ethnobotany of Rue (Ruta graveolens L.): an overview Giovanni Aliotta1, Gennaro Cafiero2, Anna Oliva1, Edoardo Pinto3 1Dipartimento di Scienze della Vita, Seconda Università di Napoli, Via Vivaldi, 81100 Caserta, Italia; 2C.I.R.U.B., 30rto Botanico, Università di Napoli Federico II, Via Foria 223, 80139 Napoli, Italia. Abstract. Uses and properties attributed to Ruta graveolens L, an ancient medicinal plant, suggest a modern scientific approach that allows to fully evaluate thè applicative potentiality of allelochemicals of this species both in medicai and ecological fields. Riassunto. Gli usi e le proprietà attribuite a Ruta graveolens L, un’antica pianta medicinale, suggeriscono un approccio scientifico moderno, che consenta di valutare appieno le potenzialità applicative degli allelochimici di questa pianta, sia in campo medico che ecologico. Key words: Ethnobotany, Naturai pesticides, Pharmacognosy, Ruta graveolens Thè medicinal plants mentioned in ancient texts deserve modem scientific investigation. Indeed, we are lulled into neglecting an important source of information: history. History of thè plant Rue (Ruta graveolens, Rutaceae) is an evergreen plant native of Southern Europe with bluish-green leaves that emit a powerful odour and have a bitter taste. Thè plant is cited in thè ancient herbals and has deep roots in folklore, alchemy and even demonology. Rue has been regarded from thè earliest time as successful in warding off contagion and preventing thè attacks of fleas and other noxious insects. Thè name rue derives from thè Greek “reuo” (= to set free), because thè plant is efficacious in 64 various diseases. Rue was thè chief ingredient of thè famous antidote to poison used by Mithridates (Grieve, 1967).
    [Show full text]