N B A G R N B A jkG iR vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G Rokf"kZd izfrosnujk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R ANNUAL REPORT N B A Gjk iR vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R 2014 - 15 jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A Gjk iR vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vkjk-i-vk-la-C;wjks la C;wjks N B A jkG iR vk la C;wjks N B A G R N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A G R N B A Gjk iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A jkG iR vk la C;wjks jk i vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R HkkÑvuqi&jk"Vªh; i'kqjk vkuqoaf'kdi vk la C;wjks lalk/ku C;wjks N B A G R jk i vk la C;wjks N B A G R djuky & 132001 (gfj;k.kk) HkkjrN B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A G R jk i vk la C;wjks ICAR-National NBureau B A of AnimalG R Genetic Resources Karnal - 132001 (Haryana) India N B A jkG iR vk la C;wjks N B A Gjk iR vk la C;wjks jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A G R jk i vk la C;wjks N B A jkG iR vk la C;wjks N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks jk i vk la C;wjks N B A G R N B A G R jk i vk la C;wjks N B A G R ANNUAL REPORT 2014-15 343, 1 Intech Printers&Publishers Printing Sh. Satpal,Tech.Officer Ms. InduBala,StenoGr.III Dr. PSDangi,A.C.T.O. Assistance I/C, P&EUnit Photographs Dr. IndrajitGanguly,SeniorScientist Dr. SaketKumarNiranjan,SeniorScientist Dr. AKMishra,PrincipalScientist Dr. RSKataria,PrincipalScientist Dr. NKVerma,PrincipalScientist Editors & I/C,PMECell Dr. NKVerma,PrincipalScientist Compiled by Karnal -132001(Haryana)India ICAR-National BureauofAnimalGeneticResources Dr. ArjavaSharma,Director Published by Line... Credit E-mail: [email protected] Tel: 0184-4043541,3292951 Karnal -132001,Haryana jk-i-vk-la-C;wjks st Floor,MughalCanalMarket, NBAGR

Network Project on AnGR Awards 54 Patents &Technologies Publications Research Projects Research Projects and Publications Ex-situ CharacterizationGenetic &Functional Genomics Phenotypic Characterization Livestock Information Management Research Accomplishments Financial Outlay Organogram 8 Divisions' Profile About Bureau History and Profile Executive Summary Foreword jktHkk"kk izdks"Bdhxfrfof/k;k¡ dk;Zdkjh lkjka'k izkDdFku fgUnh [k.M Superannuation 85 Joining/Transfers 85 Promotions 85 Administrative Staff Technical Staff Scientific Staff Personnel Visits Abroad Distinguished Visitors Swachh Bharat Abhiyan Celebrations 77 Important Meetings 77 Library Other Activities Exhibitions Organized Trainings Attended Trainings and Workshops Organized Training and Capacity Building Approved RFDEvalutation 2013-14 Report Approved RFD2014-15 Result Framework (RFD) Document Conservation

i

Contents

98 89 89 37 37 54 47 45 24 15 13 84 83 83 79 78 78 77 72 72 71 66 56 iii 9 4 3 I sheep, Poonchi sheep, Tibetan sheep, Tibetan sheep, Poonchi sheep, Marwari cattle, Kosali cattle, Assamese cattle, Binjharpuri cattle, Purnea of Network Project on AnGR, characterization the Under chicken. of line parent male PD1(Vanraja) and chicken Mewari pig, Goan Agonda camel, Kharai goat, Pantja Gangatiri cattle, Katchaikatty black sheep, and Belahi included, These registered. were chicken of line parent male one and poultry and livestock of breeds new seven programme, registration breedUnder breed. the of improvement and conservation supporting for tract breeding its in utilized been also has semen buffalo Jaffarbadi Bureau. the of Bank Gene the to added were (Toda) buffalo and Tharparkar) and (Gaolao cattle of doses semen 7600 program, is in progress. Under ex-situ conservation Sikkim Black goat and Kaunayen chicken sheep, Yalaga and Mouli cattle, Siri of characterization The dogs. Chippiparai and Rajapalyam goat, Singharey sheep, Kajali included which livestock, indigenous of populations known lesser some of characterization phenotypic the completed we year, the During resources. genetic animal farm Indian of attributes different with associated characterization of the genetic potentials andidentification for hard striving been has institute the 1984, in inception its and achievements of this Institute. Since containing a brief account of the activities 2014-15, period the for (Haryana) Karnal Resources, Genetic Animal Report of ICAR- National Bureau of Bureau National ICAR- of Report t is my pleasure to present the Annual the present to pleasure my is NBAGR FOREWORD screening ofbreeding bulls. cytogenetic for banks semen various to continued to provide consultancy services signature with high accuracy. The Institute generated and analysed to develop breed was loci STR 4 using Tharparkar) and Sahiwal (Gir, breeds 3 from individuals farm 541 on Data breeds. indigenous different in characterized were etc.) MCT,CD147(Myostatitn, endurance and etc.) Kiss1 BMP15, (GDF9, reproduction etc.), Ankirin Titin, CAST, CAPN1, Leptin, PIT1, GHR, GH, (DGAT1, production for Ladhakhi cattle. Various candidate genes evolved naturally the in influence taurine of absence near the highlighted traits milk to related genes Candidate progress. in is buffalo Chattisgarhi and Odisha of characteriztion genetic while level molecular at sheep Koraput in observed was diversity genetic High was undertaken. chicken Harringhata and cattle Ongole Assam and conservation of Bargur cattle, of Ducks chicken, Hazra Rajasthan, of Donkeys mithun, and yak Arunanchali camel, Jalori Assam, of population pig goat, Bhakarwal goat, Bundelkhandi jk-i-vk-la-C;wjks

i ANNUAL REPORT 2014-15 ii ANNUAL REPORT 2014-15 this, a number of distinguished persons distinguished of number a this, the capacity of Expert Lecturer. Apart from participants of IAEA-sponsored training in international the to training impart to (Austria) Vienna visited I organized. was of Indigenous Animal Genetic Resources” Approaches for Genome Characterization Bioinformatics and Molecular “Advanced (Sikkim). ICAR sponsored short course on Gangtok Board, Development Livestock Sikkim with collaboration in Gangtok at organized was state Sikkim of Resources brainstorming session on Animal Genetic A lectures. and sessions brainstorming exhibitions, holding by generated was AnGR on masses among Awareness (NAVS). Sciences Veterinary of Academy National and ICAR with collaboration in organised was cattle” indeginous of productivity milk enhancing for “Strategies on meet consultation expert An time. in held were meetings IRC jk-i-vk-la-C;wjks NBAGR always welcome. are improvement the for suggestions and Feedback report. this in reflected been have contributions whose bureau the of members staff other and officers all the scientists, technical, administrative congratulate I satisfaction, and pride of development of this Institute. With a sense overall the for guidance and support constant their for PS Bhasin, Vineet Dr and (AP&B) ADG Gandhi, S R Dr (AS), DDGPathak, L M K Dr ICAR, DG, & (DARE) Secretary Ayyapan, S Dr to grateful am I Bangladesh. and USDA from teams (Kenya), Nairobi Smith, Jimmy Dr. included which Institute the visited Director General, ILRI, General, Director (ARJAVA SHARMA) DIRECTOR set upset on 21 and National Institute of were Animal Genetics National Bureau of A Lakhs. againstwas Rs.21.68Lakhs target the Rs.22.00 Receipt Revenue of generated Rs.180.00Lakhs. included against release of Rs. 170.00 Lakhs, total expenditure which was Rs.178.72Lakhs, year of 2014-2015. Under Network project, total receipt during of financial Rs.1186.00Lakhs (combined) against was Rs. 1179.332 Lakhs the Total expenditure under Non-plan and Plan y y y y country’. The objectives the of Institute are- of livestock genetic resources and poultry of the characterization, conservation and utilization with mandate the ‘Identification,evaluation, organization nodal the has been in India National Bureau of Resources Animal Genetic Bureau of Resources Animal Genetic in1995. merged to as function asingle unit as National Institutein 1994.Both, and Bureau the were shifting to its campus own at Makrampur, Karnal temporarily housed inNDRI Campus before institute were shifted then to Karnal in1985 and Research Institute, Bangalore. Bureau and the started at Regional Station of National Dairy y y y y of animal genetic resources. evaluation, characterization and utilization To conduct training programmes as related to of molecular biology. characterization using techniques modern To studies undertake on genetic resources. optimal utilization of farm animal genetic conservation and in-situ management and To for methodologies design ex-situ Nationaltheir Data Base. genetic resourcespoultry and to establish evaluate and catalogue farm livestock and To conduct systematic to characterize, surveys NBAGR st September, institutes 1984.These nimal Genetic Resources Genetic nimal EXECUTIVE SUMMARYEXECUTIVE sized withphenotypic wide sized variations. Animals weightbody were recorded. Mouli sheep are large Karnataka state. and Data biometry on body of Mouli and Yalaga sheep populations of for was undertaken characterizationA survey animal is docile. ground. Udder is small.Temperament of the orientation. Tail is long, touching almost to to moderatesmall in length and horizontal in Humpcurved. and dewlap are Ears small. are brown and black with white patches. Horns are colour area. of Body animals isLadakh the black, characterized phenotypically through surveying cattle adaptedLadakhi to altitude high was plough about one acre of land in6-8hours. forused draftpairand a purpose of maybullock yield ranges from are2.0 to Bullocks 6.50 kg. up to hock. Udder the is The small. daily milk white spots to black. Ears are Tail small. reaches colour ofBody cattle Siri varies from brown with morphometric characteristics and performance. different age sex and were recorded for physical, of Atotal of district Sikkim. East 68animals of conductedwas characterized through in survey Indigenous cattle of known Sikkim as “Siri” by Bureau. the One chicken registered line was also firsttime indigenous breeds is now country inthe 151. registered newly these breeds, total number of Mewari chicken of Rajasthan. After including camel of Gujarat, Agonda pig Goan of Goa, Katchaikatty Black sheep of Tamil Nadu, Kharai Pantja goat of Uttarakhand and Uttar Pradesh, Gangatiri cattle of Uttar Pradesh and Bihar, cattleare of Belahi Haryana and Chandigarh, camel and chicken were registered. The breeds of cattle, one breed each of goat, sheep, pig, breeds new ofSeven livestock -two breeds jk-i-vk-la-C;wjks

iii ANNUAL REPORT 2014-15 EXECUTIVE SUMMARY iv ANNUAL REPORT 2014-15 EXECUTIVE SUMMARY 66.3% to three 71%inthe ecotypes. components with atotal variance ranging from 14 morphometric traits.two The PCAextracted SolapuriMadgyal, and Kolhapuri using ecotypes Component Analysis (PCA) was on performed five in distinct ecotypes groups. Principal areas. Discriminant Analysis the classified sheep were from collected distribution their Sangamneri, Lonand and Kolhapuri) of Deccani merits of Solapuri, five (Madgyal, ecotypes reproduction status, threat status and breed data on production survey Interview based and Average greasy wool production is 800to 1000g. to ground.sexesboth The are polled. generally long and pendulous ears and long touching tail in sizewith well-built body, having Roman nose, or dark brown faceand ear. The animals arelarge White (Chitti) Kajali -with and white body black with complete black or black- brown and body 2) on basis of the colour – 1) Black (Kali) Kajali- /colourtypes variants, distinguished primarily respectively.43.23 ± 0.36 kg, Kajali sheep has two weights of and males females are 56.98±1.02and and adjoining of districts Punjab. The Adultbody distributed in Sangrur, Ludhiana, Barnala, Moga Kajali, amutton and sheep was surveyed type and females are polled. are present animals. the Males inall are horned are white. Muzzle is black brown or pink. Wattles complete black. Extremities, except faceand ears, with black or brown patches sizeto of varying present region. thigh inthe Face is white, white colour consisted hair. thick small Ahair tuft is Yalaga sheep are medium sized. White coat sexes arethe polled. typical Roman or comparatively straight. Both flocks type. were typical Noseline is Madgyal A brown ring is present around eyes.Afew the is white with or without brown spots/patches. andare body with long deep tall colour legs.Coat jk-i-vk-la-C;wjks NBAGR The age maturitysexual at indogsrangesfrom medium andposition. inerect flat, few with very dark brown, brownish black and black. Ears are coat colorin sizewith varying from fawn to characterized. Chippiparai dogs are medium ofdistricts southern Tamil Nadu was Madurai, Virudhunagar and Thoothukudi Chippiparai dogdistributed inTirunelveli, is brown. size with an average of Shell 42.43±.07g. colour are fighting type birds. Eggs were medium in production is about annum. 35to 45per These weight is Body about type. pea 2.5to Egg 3kg. golden feathers. Comb is red incolour and is of is black or brown with patches of white, black or chicken “Kaunayen”.local called Plumage colour Imphal West of districts Manipur to characterise conducted has been Survey inThoubal and mainly for meat purpose. than Singharey goats. goats Sikkim are reared Singharey goats. These goats are slightly shorter body. Face, horns and are tail longer than that of are of medium size. Head is proportionate to Black size. goats Sikkim body intheir Bengal of horns and sizeand from their also Black Singharey goats of inrespect coat colour, type of colours. these These goats arefrom distinct coat colour brown, black, white, and mixture goatSikkim germplasm constitute goats with goats.these black or white. Black top inmany line is seen of brown or white. are Legs short, stout, medially round tip. The under is belly generally light ears are short to medium pendulous semi with eyes The stripes. facial are bright and small. The distinguished from other populations by the goat ofpart Sikkim the population and can be goats. Singharey goats constitute major the goat population- Singharey and Black Sikkim was conductedSurvey to characterize Sikkim the signature. F was generated and to analysed develop breed (Gir, and Sahiwal Tharparkar) using 4 STR loci Thedata on 541farm individualsfrom 3 breeds average inbreeding coefficient (F) was 0.037. indicating sufficient genetic variability. The heterozygosity(0.75) and expected (0.79), heterozygositynumber of (4.84),observed alleles numberof of (9.95),effective observed alleles various within breed diversity measures in terms with mean number The locus. of per 9.95alleles of were 200alleles across detected 20loci the genetic variability cattle. in Ladakhi A total across 20markers was analysed to estimate the Microsatellite genotypic based data recorded hobby.their breeders for guarding and hunting as well as for 12-16 months. These animals are kept theby joining network on analysis haplotypes based resulting in57haplotypes.observed Median swamp breeds/populations, 81variable sites were comparative analysis across nine riverine and was amplifiedbuffaloes and sequenced. By region of 30Kalahandi and 19Paralakhemundi 50 chromosomes. 811bp mitochondrial D-loop cytogeneticriverine buffalo constitution with and Paralakhemundi, typical revealed which Cytogenetic study out was carried for Kalahandi assignment. for Sahiwal, Gir and Tharparkarcattle breeds kit. Thekit was tested and validatedbe used to loci wereThese to develop used a multiplex PCR assigned and 3breeds formed different clusters. organized farms were found correctly to be samples. field the added the individualsfrom All farmsthe of three breeds these and 93%when able to assign 100% individuals belonging to of was attempted loci and finally eight were loci andSahiwal Tharparkar, respectively. Selection Gir andbetween Sahiwal, Gir and Tharparkar and NBAGR ST values were 0.173,0.281and 0.235 a frequency ofa frequency 0.40,0.40and 0.19;respectively. were genotyped. GG,GCand genotypes CC had primers and 42samples of Madras the Sheep Red nt position SNP (G/C)using specific allele the Genotyping protocol was developed for 112 the position promoter inthe region of HSP90AA1. protocol was developed for -660and -601nt 248 nt position. The tetra- ARMS PCR primer region three revealed novel SNPs at 112,244and amplicon size of 499 bp was amplified. The containing 7already reported SNPs with an were studied. Promoter region of HSP90AA1 genes infour different breeds of Indian sheep SNP markers heat inthe shock protein (HSP) andDQB2 DQB3. corresponding to three major groups i.e. DQB1, Foralleles). DQB, total were 16 alleles identified major groups, DQA1 and (11alleles) DQA2 (2 swampwhich corresponded buffaloes, twoto A total of 13DQA were alleles identified in found polymorphic. to be technique. Only Exon 40of FASN gene was in Chhattisgarhi buffalo, using PCR-RFLP UTR of STAT1 were studied also for variation traits -FASN (Exon 38,39,40,41,42)and 3’ Thecandidate genes the affecting fat production with chromosomalriverine type number fifty. analysis of Chhattisgarh buffalosamples revealed Chhattisgarhi buffalo were recorded. Cytogenetic biometric measurements on about 140 adult Phenotypic characterization was done and Kawarda and of Baster districts Chhattisgarh. of Dhamtari, Kanker, Mahasumund, Bilaspur, Pilot surv breeds. populations grouping with other riverine buffalo sharing, shown has also Odisha the buffalo both eys wereeys conduct inabout 41villages jk-i-vk-la-C;wjks

v ANNUAL REPORT 2014-15 EXECUTIVE SUMMARY vi ANNUAL REPORT 2014-15 EXECUTIVE SUMMARY late lactation periods. lactation (10to 30days) compared to midand and GPAM etc. was significantlyearly in higher lactation stages. Expression level of SCD, FABP3 early lactation and inlate gradually decreased abundance level was significantly high during compared to midand late lactation. Their mRNA duringhigher as early lactation and period peak cattle. Expression pattern genes of casein was all ACACA, SCD, GPAM) were evaluated inSahiwal and fat metabolism genes (FABP3, BTN1A1, (CSN1S1, CSN1S2, CSN2, CSN3, LALBA), The expressionpatterns casein genes the milk of of different proteases earlyduring lactation. lactation. The analysis showed lower expression an increase inexpression during course of encoding different endogenous proteases showed Murrah buffaloeswas studied.the All genes various lactation stages in Sahiwal cows and Expression of endogenous proteases across ofpart lactation. and early lactation stages as compared to later stages of lactation and are incolostrum higher that antioxidant levels change during different mid and late lactation stages. Data suggested and early lactation stages compared to peak, of DPPH was significantly incolostrum higher Murrahpercentage buffaloes, scavenging activity stages and inSahiwal Karan Fries cows. In (DPPH) showed no difference acrosslactation activity of 1,1-diphenyl-2-picrylhydrazyl stages. other Onthe side, percentage scavenging followed by early, midand late peak, lactation was significantly incolostrum (p<0.05)higher Sahiwal and Karan Fries cows, TAC of milk duringmilk lactation incattle and buffalo. In (TAC) and scavenging radical free activity of comparative changes intotal antioxidant capacity Study was to conducted to evaluate the jk-i-vk-la-C;wjks NBAGR compared to cows. Sahiwal significant induction in Holstein Friesian cows HSP90 genes together showed maximum and expression values for HSP70, HSP40, HSP60 and expression with change Combined inseason. showed non-significant or minimum change in determined. The HSPtranscripts cows inSahiwal Holstein Friesian cows and Murrah was buffaloes of major chaperones in PBMCs of Sahiwal, Impact of variation seasonal on transcription summer stress. cellular tolerance than exotic, crossbred cows to cowsSahiwal and Murrah buffaloes havebetter MurrahData buffaloes. providedthatevidence Fries cows, lower while cows inSahiwal and was Holsteinhighest in both Friesian and Karan physiologicalthe stress inbovines. The N/Lratio lymphocytes (N/L) ratio was evaluated to assess Comparative evaluation of neutrophils/ cattle. inLadakhi were observed breeds and at BTN-3 novel new loci, variants reported in anybeen other Indian native cattle Variant at κ-CNthat Ewas observed has not characteristics at most of studied the loci. cattle haveLadakhi maintained indicine the DGAT-1 across cattle. 72animals of Ladakhi bGH,Pit-1,β-LG,α-LA, PRL, BTN-1,-3 and important candidate genes- κ-CN,β-CN, Distribution pattern of variants allelic at respectively.D allele; of PRDM9 gene was 6,7,8and 9inA,B, Cand recorded. Number of zinc fingersthis domain in CD, AC and BD) for PRDM9 gene have been and nine genotypes (AA,BB, DD, CC, AB, BC, and crossbred cattle. Four (A,B, alleles Cand D) PRDM9 gene was studied inindigenous, exotic variation in zinc finger domain of bovine To decipher subfertility inbovines, sequence carried out.carried in Tibetan sheep by microsatellite markers was Project centers. Assessment of genetic variability chicken out was carried by respective Network of Bargur cattle, Ongole cattle and Harringhata Duck of Assam out. was carried Conservation Yak, Donkeys of Rajasthan, Hazra chicken, population of Assam, Jalori camel, Arunachali Binjharpuri, Assamese, Kosali cattle, pig Poonchi Sheep, Tibetan sheep, Mithun, Purnea, of Goat, Budelkhandi, Marwari, Bhakarwal Under Network project, Characterization and Yak). (Cattle, Buffalo,Goat, Sheep,Camel, Equine belonging to forty four breeds of species seven NBAGR now stores 1,29,174 frozen doses semen during last year. The NationalBank Gene at procured and to added repository Bank in Gene and Tharparkar) and Buffalo (Toda) been have Total 7,600 frozen of doses Cattle semen (Gaolao prepareddoses using protocol. this caprine epididymal and semen frozen semen protocol standardized has been for freezing of yolkegg and antibiotics along with acooling An extender containing citrate buffer, sugar, genes. and HMBSare most the stable internal control have demonstrated that RPS15,GAPDH, RPS9 geNorm,methods NormfinderBestKeeper and >EEF1A1 >UXT >ACTB >HPRT. Lgorithmic RPS9=RPS15>HMBS >GAPDH>B2M >RPL4 arranged indescending order of stability: stability and stepwise exclusion, genes were tested. basis of Onthe relative gene expression to altitude high and tropically adapted were ICG during studies inIndian cattle adapted categoriesfunctional as suitable that could serve Ten well-known reference genes from different NBAGR conferences. and attended workshops, the symposia and Scientists deputed for training within country the IRC meeting. research projects was during reviewed midterm meetings were held on time. The progress of The Institute Research Committee (IRC) indigenous animals. and sensitize farmers about of benefits the various places to showcase institute’s activities Nine exhibitions on AnGR were organized at 10, 21,195/-were procured library. inthe andwere journals worth Rs. Books subscribed. (11foreignjournals and 31Indian journals) current scientific developments, / technical 44 In Bureau the library, to keep track of the of 99.50%and received excellent rating. Under RFD, NBAGR acomposite achieved score Office, Delhi. New applications were submitted to Indian the Patent first examination requests for three patent Journalthe of Indian Patent and the Office patentSeven applications were published in were published also by institute. the published. 12 Monographs and training manuals impact factor. 27 technical/popular werearticles National and International Journals of high Total 43 research papers were published in National Fellow projects are on-going. including one and funded externally one during last year. At present 21 research projects A total of 11Research projects were completed parameters.cytogenetic buffalo bulls and 40 pigs werescreened fortheir year the During atotal of 113breeding cattle and of Government policy the as per defects of India. anddefects to keep herds the of free such genetic aimthe to check spread the of chromosomal various agencies throughout with country the screeningcytogenetic of breeding to males NBA GR is providing of consultancy service jk-i-vk-la-C;wjks

vii ANNUAL REPORT 2014-15 EXECUTIVE SUMMARY viii ANNUAL REPORT 2014-15 EXECUTIVE SUMMARY Indigenous Resources” Animal Genetic was Approaches for Genome Characterization of “Advanced Molecular and Bioinformatics Ten days ICAR sponsored short course on LivestockSikkim Development Board, Gangtok. 19.11.2014 at Gangtokin collaboration with Resources of state Sikkim was organized on A brainstorming session on Animal Genetic and NBAGR on 28.01.2015at NBAGR. Process’ was organized jointly by DAHD&F, GOI An interactive workshop on ‘Breed Registration with CAU, Imphal. at Keishamthong, Imphal incollaboration (W) Manipuri Chicken’ was organized on 21.11.2014 One day workshop on ‘Characterization of to application” was conducted. generation sequencing analysis: from technology A three day Training program entitled “Next at NBAGR campus. Diwas, ICAR Foundation day were celebrated Republic Day and Independence Day, Taru Foundation Day of Bureau, Biodiversity day, jk-i-vk-la-C;wjks NBAGR technical officertechnical superannuated during year. the transferred. One principal scientist and one of post the Director, ICAR-CIRC, Meerut and One principal scientist appointed has been to grades/posts. and were staff 3skilled promoted to higher working at Institute. the Six scientist, 2technical administrative staffpersons and 5skilled are At present 17 technical, 29 scientific, 19 masters degree. Divisions of students the NDRI. guided Also for Cattle Breeding and Animal Biotechnology NBAGR scientists taught various course in Dairy Lecturer.Expert of IAEA-sponsored training capacity inthe of impart training to international the participants Director, NBAGR visited Austria, Vienna to visitedILRI also Institute. Institute. Dr. Director General, Jimmy Smith, A number of distinguished persons visited the course.the organized. Atotal of 21participants attended FinancialOutlay • Organogram • Divisions'Profile • AboutBureau • History and Profile NBAGR jk-i-vk-la-C;wjks

1 ANNUAL REPORT 2014-15 jk-i-vk-la-C;wjks NBAGR on & F u n cti a l G e no mics ati Ch aracteri z G e n etic were up set on 21 National Institute of (NIAG) Animal Genetics of Resources Genetic Animal (NBAGR) and of Institute. this Therefore, National Bureau coordinated efforts the forthe establishment 6 Dairy Research Institute,Dairy Bangalore. Bureau and institutes at started Regional Station of National T principle during 4 About Bureau HISTORY ANDPROFILE th • • • • Five Year Plan, various government agencies livestock and poultry genetic resources oflivestock thecountry. and poultry ‘Identification, evaluation, characterization, and conservation utilization of Institute of was accepted Animal Genetics in forhe need establishment the of National of animal genetic resources. To conduct training programmes as related to evaluation, characterization and utilization biology. To studies undertake on genetic characterization using techniques modern of molecular utilization of farm animal genetic resources. To for methodologies design ex-situ genetic resourcespoultry and to establish National their Data Base. To conduct systematic to characterize, evaluate surveys and catalogue farm livestock and NBAGR th Five Year Plan. 5 During st September, 1984.These OBJECTIVES th MANDATE and and conservation and in-situ management and optimal mandate and objectives as given below: organization nodal the has been inIndia with the National Bureau of Resources Animal Genetic in 1995. National Bureau of Resources Animal Genetic were merged to as asingle unit function as InstituteKarnal in 1994. Both and Bureau the before shifting to its campus own at Makrampur, and housed temporarily inNDRI main Campus institutethe were shifted then to Karnal in 1985 jk-i-vk-la-C;wjks

3 ANNUAL REPORT 2014-15 About bureau 4 ANNUAL REPORT 2014-15 Divisions profile functioning to support divisional the activities. Information Unit and Network Project Unit are In addition to two this, units namely Livestock 3. 2. 1. to achieve institute’s the mandate and objectives: 22.07.2013 following divisions have created been As ICAR per letter.No. AS 5/21/2012.IA.I dated Divisions' Profile continues inits present form. Resources Division’ and since division the then was renamed section the as ‘Animal Genetic InResources and 1997 Section. Conservation was merged intoSection Animal Genetic Animal Physiology1996, the and Reproduction Animal Physiology and Reproduction In Section. Resources c) Genetic and Section Conservation Management Unit/Computer Unit b)Animal were again reframed as: a)Information and Unit. and sections 1994-95the During units Unit and and Animal Conservation Bank Gene Management Resource Animal Genetic Section, and two units Livestock viz Information and NBAGR then 1992-93 the had one section 1991-92. Reorganization continued and in Physiology and Reproduction during section was created was which reframed as Animal In of section 1991 a new Animal Bank Gene ResourcesGenetic and Conservation Section. divisionsConservation were merged into Animal In Resources 1987,Animal Genetic and Animal into existence after a number transformations.of ResourcesAnimal Genetic (AGR) division came description The methodologies. present form of strategiescharacteristics, and survey breed engagedbeen indeveloping formats for breed ResourceAnimal Genetic (AGR) Division has Resource Division Animal Genetic jk-i-vk-la-C;wjks Animal Biotechnology Division DivisionAnimal Genetics ResourceAnimal Genetic Division NBAGR HISTORY ANDPROFILE long term ex-situ conservation of germplasm. division working is also frontier inthe areas of of various livestock In species. addition, the conservation implemented has been for breeds of breeds the under field conditions. The in situ formulated for improvement and conservation information,the strategies new have been addition to research publications. on Based breed descriptors and breed calenders in documented form inthe of breed monographs, habitat. breeds/populationsThe surveyed are recorded under agro-climatic conditions of their performance breeds of local is evaluated and of live animals and products. The production and production performance, and marketing characteristics, biometry, body reproduction and management practices, phenotypic structure, population status, feeding, breeding economic status of farmers, the flocks/herd systematic/pilot socio- to assess field surveys characterization is accomplished through livestock breeds. and The poultry phenotypic utilization and conservation of indigenous in phenotypic characterization, sustainable along with its ‘National Bank’ Gene is engaged At present Resources Animal Genetic Division abnormalities in relation to abnormal phenotypes encompassedalso research on chromosomal equines and poultry.cytogenetic The studies emphasis was laid on like camel, species, pigs, had relatively more information available, so and camel. Since cattle, and buffalo goats profile of cattle, buffalo, sheep, goat, pigs projects encompassed studies of chromosomal polymorphism studies. The research initial resources using and cytogenetics biochemical was on characterization of indigenous genetic National Institute of (NIAG) Animal Genetics The major research years ininitial thrust of Division Animal Genetics also providingalso intermof consultancy services and DBT projects. Division Animal Genetics is division has completed successfully NAIP the breeds from different livestock The species. tolerance,thermo resistance disease inour native meat, growth and development, adaptive, importance yield/ composition, milk viz; wool, candidate gene influencing loci trait functional of single nucleotide polymorphism (SNPs) at several in sequence characterization and identification of In recent years, sizeable progress made has been MHC and bovine interleukins in Indian cattle. in field the of immunology the especially on marker. Significant work continuedalso was were made using also mitochondrial D-loop relationships amongst native breeds, efforts diversity, maternal lineages and evolutionary understand nature the of mitochondrial DNA molecular markers microsatellites. especially To poultry, pigs and other livestock using species analysis of native breeds of cattle, sheep, on molecular characterization and biodiversity most of research the projects en compassed work divisionalthe research was activities observed, In year the 1997, a major change of focus in the scientists and two senior scientists at present. approved in2014.The division has fiveprincipal was established year inthe 1996and formally of part the Division, Animal Genetics which immunogenetics, and molecular genetics became scientists working fields inthe cytogenetics, of On merging of two the institutes, the all and major genes of functions. phagocytic of humoral immune response, bovine cytokines major histocompatibility complex and genetics other major studies included antigens of bovine reagents and to build anational center. blood The antigens and applications, their preparing typing included characterization of cattle erythrocyte immunogenetic and biochemical genetics and reproductive disorders. The work on HISTORY ANDPROFILE NBAGR major genetic disorders. karyotyping breeding the bulls and screening of biotechnology faculty, involved in teaching and of division the are members inNDRI also Nair scientific best worker award. Fourscientists Two of its scientists have awarded been Dr. P.G. and another from have DRDO approved. been grants one from National Fund scheme of ICAR recently division. inthe Two more external biomarkers" have completed been successfully, buffalo for identification of probable mammary lactation and involution inindigenous cattle and gland transcriptome and proteome during resistance"in disease and "Analysis of mammary animals: evolutionary lineages and application research projects "Toll-like receptors in farm projects.as externally funded Two NAIP funded andfor indigenous dual-purpose dairy cattle" mining and development of breed signatures a DBT project on "Whole genome SNP based environments innative cattle and buffaloes" and of tolerance thermo and adaptation to diverse "Genome data mining to unravel basis molecular currently, has one National Fellow project on than institutionalOther projects, division the unique attributes of indigenous livestock species. production related traits, inorder to explore the in adaptation, resistance disease and various evaluation of genes, and transcripts involved genomic tools for identification the and livestock major species, emphasis is on utilizing of genetic characterization work on various mandated assigned tasks activities. Besides staff, working indiverse fields per institute's as scientistsseven supported by four technical orders. Present strength of Division the is DNA Fingerprinting Unit by Council's the existence w.e.f. 16.01.2014from erstwhile the Animal Biotechnology Division came into Division Animal Biotechnology jk-i-vk-la-C;wjks

5 ANNUAL REPORT 2014-15 Divisions profile 6 ANNUAL REPORT 2014-15 Divisions profile Prevailing • division are: division. Major scientific achievements theof from themeat filed been and has also milk patent on differentiation cattleof and buffalo programme for researchers of NARES. One repute field inthe as well as organizingtraining work invarious international journals of high regularlyhas been publishing quality research guiding of Masters and Ph.D. students. Division poultry genetic resources.poultry looking afteris also registration of livestock and Knowledge Network inJune section 2014.The provided has Line been throughLeased National was upgraded to 20Mbps in June 2014. Another to institute. the Line connectivity Leased The provides Internet LAN, and computing facilities in an retrievable easily format. also section This on animal resources and to provide it to users the This Unit is engaged in digitization of information InformationLivestock Management Unit Identification • Transcriptome • New • jk-i-vk-la-C;wjks established. cattle,species- buffalo, sheep and goat genetic relationships of indigenous livestock cytogenetic toolscytogenetic and microsatellite markers. using mitochondrial D-loop sequencing, northeast Indian states, accomplished swamp, riverine and hybrid in buffaloes documented. diversityallelic across breeds/populations resistance, meat quality, reproduction and traits of economic importance like disease adaptation to stress and production. milk helping inidentification of genes influencing alleles/SNPs genetic analysis and NBAGR identified status distribution of buffalo and for interbreed and of various cattle pure

HISTORY ANDPROFILE (Krishna Valley, Ponwar, Kherigarh, Kangayam, ofConservation AnGR includes 5 cattle breeds Arunachali Mithun. Ankleshwar), Kutchi Camel, Spiti Horse and Ganjam, Mehsana), 2 breeds of chicken (Aseel, breeds of goat (Osmanabadi, Barbari, Attapady, Banpala, Coimbatore, Chhota-Nagpuri), 5 Deccani, Mecheri, Mandya, Rampur Bushair, Nagpuri, 8 breeds Surti), of sheep (Changthangi, Gangatiri), 3breeds of (Jaffarabadi, buffalo Dangi, Amritmahal. Khillar, Gaolao, Tho Tho, (Deoni, Ongole, Gir, Umblachery, Bachaur, of breed descriptors of 11breeds of cattle and genetic characterization &development The updated achievements include phenotypic for started has been conservation. characterization and work on 4 at risk breeds breeds/populations have for undertaken been up. taken also current During XIIplan 17new NEH region and buffalo genomics work was XI plan one more core lab was established in were initiated also from IXplan onwards. In labs. In-situ and ex-situ conservation activities commenced inIXplan by establishing 3core of breeds. characterization Genetic was also each plan were for undertaken characterization of breeds. In IXand Xplan centers 12new in There were 8centers in VII plan for characterization • • • • followingthe objectives: The Network project was initiatedwith in1996 Network Project Unit To germplasm the conserve To develop breed the descriptors candidate gene studies in indigenous breeds Molecular genetic characterization and qualitative and quantitative traits To characterize breeds the interms of both of identified SNPslarge in populations. PCR-RFLP have established been for genotyping database of various Various species. of loci new The novel SNPs havethe added to been SNP with traits inbuffalo,cattle, goat and horses. genesin these were identified and associated diffrent regions. The Indianspecific breed SNPs characterized in indigenous breeds in core labs of MCT, CD147,IGF, ACTN, ADRB2) were BMPR1B, Kiss1 etc.) and endurance (Myostatin, etc.),Akirin, reproduction (GDF9, BMP15, PIT1, CAPN1, Leptin, CAST,GH, GHR, Titin, Various candidate genes for production (DGAT1, horse. Spiti and Nilgiri, Kilakarsel), 2 goat Surti) breeds (Beetal, Jaffarabadi, Toda), 3 sheep breeds (Magra, Nagori), 3 breeds buffalo (Pandharpuri, HISTORY ANDPROFILE NBAGR structural variantsstructural buffalo inthe genome. The present analysis unveils also about 300 of cattle 52million mismatches/indels. revealed genome comparison assembly this between and genome covered is fully by a single contig. Whole assembly has 185,150 contigs. The mitochondrial comparison to cattle the assembly Btau 4.0.The assembly represents ~91%-95%coverage in assembly has read depth of 17-19X.The buffalo genome (Btau 4.0assembly) as areference. The mate pair short read sequencing using cattle the was constructed with Illumina paired end and 30 fold coverage. The first version of assembly one female Murrah buffalo providing nearly DNA sequence data generated has been from UnderGenomics, Buffalo morethan 100GB jk-i-vk-la-C;wjks

7 ANNUAL REPORT 2014-15 Divisions profile 8 ANNUAL REPORT 2014-15 ORGANOGRAM jk-i-vk-la-C;wjks NBAGR HISTORY ANDPROFILE *Includes of Releases Rs.175.00lakhs Financial Outlay HISTORY ANDPROFILE 03. 02. 01. No. Sr. 7. 6. 5. 4. 3. 2. 1. Sr. No. Sr. Budget Estimate (in Lakh) forBudget Estimate Plan, Non-Plan (inLakh) &Network Project of NBAGR Grant TotalGrant Pension &Retirement benefits Total Revenue v) Miscellaneous expenses iv) Administrative Expenses iii) Research &Operational expenses ii) Traveling Allowance i) Establishment expenses Revenue Total Capital ii) Other capitalii) Other expenditure i) Works Capital HEAD NBAGR along expenditure with for 2014-15 thefinancial year Others Misc.Others Revenue Receipts Total Sale ofSale farm Produce Sale ofSale Technology Hostel and Guest house rent Training Programs -Income Licence feeLicence Sale ofSale Publication &Advertisement Head of Account Revenue Generated during theyear 2014-15 Revenue Target Fixed :22.00Lakhs Target :21.68Lakhs Achieved 916.00 70.00 836.00 6.50 75.50 43.00 4.00 707.00 10.00 10.00 Receipt 0.00 NON-PLAN 910.94 67.54 834.70 6.27 75.33 43.00 4.00 706.10 8.70 8.70 Expenditure 0.00 21,68,127 7,60,015 5,84,000 69,550 2,98,670 1,88,868 2,04,209 62,815 Amount (Rs) 270.00 0.00 194.00 2.00 107.00 75.00 10.00 0.00 76.00 36.00 Receipt 40.00 PLAN 268.38 0.00 193.97 2.00 106.99 74.98 10.00 0.00 74.41 34.44 39.97 Expenditure (Rs.In Lakhs) 180.00 0.00 180.00 0.00 0.00 0.00 0.00 0.00 180.00 0.00 0.00 0.00 Receipt Network Project jk-i-vk-la-C;wjks 178.72* 0.00 178.72 0.00 0.00 0.00 0.00 0.00 178.72 0.00 0.00 0.00 Expenditure

9 ANNUAL REPORT 2014-15 FINANCIAL OUTLAY 10 ANNUAL REPORT 2014-15 FINANCIAL OUTLAY jk-i-vk-la-C;wjks NBAGR HISTORY ANDPROFILE NetworkProjectonAnGR • ExsituConservation • GeneticCharacterizationandFunctionalGenomics • PhenotypicCharacterization • LivestockInformationManagement • Research Accomplishments NBAGR jk-i-vk-la-C;wjks

11 ANNUAL REPORT 2014-15 jk-i-vk-la-C;wjks on & F u n cti a l G e no mics ati Ch aracteri z G e n etic these cattle.these is about Incidence 1000kg. is less of in disease yield is 3.25kg anddaily milk yield lactation milk to medium shaped sickle sized horns. Average is straight and broad with prominent Small poll. with white face, neck and dewlap region) Head colour patternBody is unique -reddish brown migratory and pastoral management system. Chandigarh. are These reared by Gujjars in Yamuna Nagar of Districts Haryana, and Naraiangarh, Ambala, found inPanchkula, draught) cattle breed and (milk dual purpose Cattle: ItBelahi is a sheep, pig, camel and chicken. After including two breeds of cattle, one breed each of goat, and onepoultry line of chicken. This includes registration of breeds new seven of livestock and on 6 on Breed registration Committee inits meeting Livestock Information Management Resear 01 CHICKEN-Synthetic 01 CHICKEN 01 PIG 01 CAMEL 01 SHEEP 01 GOAT 02 01 CATTLE S.N. th January, 2015 at New approved Delhi NBAGR Male Line) PD1 (Vanaraja Mewari Agonda Goan Agonda Kharai Black Katchaikatty Pantja Gangatiri Belahi Breed ch Accomplishents Research, Hyderabad Directorate of Poultry Rajasthan Goa Gujarat Tamil Nadu Pradesh Uttar and Uttarakhand Uttar Pradesh and Bihar Haryana and Chandigarh Home Tract cattle are well adapted in low/medium input dewlap are to medium small insize. Gangatiri middle. Ears are long and horizontal. Hump and Forehead is straight with shallow groove inthe directed upwards and inwards with pointed tips. and switch tail are generally black. Horns are ofdistricts Bihar. Eyelids, muzzle, hooves Pradesh, and Bhabhua, Buxar and Bhojpur Mirzapur and adjoining of districts Uttar Varanasi,Ballia, Mau, cattle found inGhazipur, white, medium sized are white or greyish Gangatiri Cattle: These Hyderabad for registration. a line of chicken PD1 by developed ICAR-DPR, strains of chicken for first the time and approved committee considered also registration of lines/ 3 for pig, 1for donkey and 16for chicken. The 40 for sheep, 6 for horses & ponies, 9 for camel, include 39for cattle, 13for buffalo, 24 for goat, indigenous breeds is 151,which country inthe registered newly these breeds, total number of INDIA_CHICKEN_001_PD1_13001 INDIA_CHICKEN_1700_ MEWARI _12016 INDIA_PIG_3500_ AGONDAGOAN _09003 INDIA_CAMEL__0400_ KHARAI _02009 KATCHAIKATTYBLACK_ 14040 INDIA_SHEEP_1800_ INDIA_GOAT_2420_ PANTJA _06024 INDIA_CATTLE_2003_GANGATIRI_03039 INDIA_CATTLE_0532_BELAHI _03038 Accession Number jk-i-vk-la-C;wjks

13 ANNUAL REPORT 2014-15 LIVESTOCK INFORMATION MANAGEMENT 14 ANNUAL REPORT 2014-15 LIVESTOCK INFORMATION MANAGEMENT swimming capacity water insea and graze as well ecosystems. as coastal have They excellent Gujarat. These are adaptedwell both dry-land to Kachchh of district camel comprises of breeding of tract this The Camel: Kharai festivals or sports. Animals are well known for ram fighting during and slender. Adult weight body is about 34kg. and outwardly twisted horns. Tail is short Female have males are while polled widespread Ears are short and stumpy. Eyes are prominent. with depression. Forehead is moderately broad. hairy. Face is of moderate length and concave compact and body black incolour. is type Coat are medium in size, Tamil Nadu. Animals Madurai of district Vedipatti taluka of in flocks in small Katchaikatty These are Black Sheep: maintained (twining >65%)goats. backwards. These areearly maturing and prolific Horns are triangular and twisted, upwards and face. Head is slightly convex. Ears are pendulous. ventrally with white streak on either sideof the is brown to fawn dorsally and lighter colour characteristics resembling deer. colour Body of U.P. These goats have morphologicalpeculiar ofdistricts Uttarakhand, and adjoining districts consist of Udham Singh Nagar and Nainital Tarai region. The home of breed tract this climate conditions of goats adapted to harsh (meat purpose and milk) are medium dual sized Pantja These Goat: draftability. good possess production system, medium producer milk and jk-i-vk-la-C;wjks NBAGR Resear ch Accomplishents composed of long archin of bird the from neck to lowertail back. The is feathers forming a“Shawl” or Cape over back the and grey. Males have bright gold and bronze Plumage colour varies from light to dark brown, is about 15.Birds are medium insize. to small range/free scavenging system. Average size flock forboth and egg meat with little or no input i.e. Rajasthan. These birds are rearedbackyard in Chittorgarh and Pratapgarh of districts Banswara, Rajsamand, Bhilwara, Dungurpur, Sirohi, Jaipur, Udaipur, breed is native to Ajmer, Mewari Chicken: region.this popular pork is which very dishindustry) in making sausage by (cottage local industry climatic conditions and preferred for sausage pigsGoan are suitable for scavenging inlocal 65kg and dressing percentage is 74. Agonda varies from 2to 12.Weight at slaughter is about patches on legs and face in Littersome cases. size These pigs are mostly black incolourwhitewith snout and are in nature wild with rough bristles. having sized small short are These South Goa. pigs of North and Goa areThese indigenous Agonda Pig: Goan Total Dissalved Solids. can thrive water on saline high and tolerate high includingskin diseases dermatitis. Kharai camel of soft clothes/stoles.Less affected by common and long for wool used can which be preparation legs during They produce walking. soft smooth, is shortpad and not does touch humerus of fore medium sizethan Kachchhi body camel. Chest Kharai camel has neck typically thick region and mainly on mangrove and other species. saline This g feathers that initially cattle area were and surveyed Siriinthe were performance. It that indigenous all was observed for physical, morpho-metric characteristics and animals of different age sex and were recorded farmers from were 5villages and interviewed 68 areas of of Atotal district of East Sikkim. 29 was conducteda survey and innorth south Regu To characterize indigenous the cattle of Sikkim, Siri cattle Phenotypic Characterization weeks of age. is 3.93%up to 6weeks and 4.88%from 7to 20 and dressing percent from 61 to 73. Mortality weight at slaughter ranges from 0.8kgto 2.1kg Average weight egg at 40 weeks is Live 55.19g. days. Hen-day production up to 52weeks is 85. 4.7kg infemales. Average age at first is 173.5 egg ranges from 2.9to and 4.7kginmales from 2.1to weight at weight 6weeks. at Body 52weeks shank length at 6weeks and 644gaverage body skin and shank, brown 74.14mmaverage eggs, comb,pea red plumage colour, yellow colour backyard poultry. The birds this lineof have line for Vanaraja commercial adual purpose shankhigher length and as parent male is used Directorate of Poultry Research, Hyderabad for developed by ICAR- in India. It has been registered line of chicken line): PD1 (Vanaraja male are broody innature. to 52.Egg weight averages about Females 53g. months. Annual production egg ranges from 37 and 1.2kginhens. Age at first is egg about 6.7 Adultin males. weight is about 1.9kgincocks of Wattles single type. are red and are large sized light.green ingood Comb is red incolour and is blacklook but shimmer with blue, purple and Resear NBAGR It is first the ch Accomplishents land in6-8hours. were Bullocks for used 3to pair of bullock may plough about 1.0 acre of yield was recordedmilk from 1.5to A 8.5kg. Under field Siri the recording program,daily yield rangeddaily milk from 2.0 to 6.50 kg. The weightbirth rangedfrom 10 to 18 kg. The gray switch. Temperament was docile. Tail was up to hock with the black, brown and round or funnel. Naval flapalmost was absent. inshape. Tipscylindrical of teats the were either were 5-12cmlong small and either funnel or Sizes of fore and rear udder were small. Teats developed and veins milk were not prominent. and upward. then Udder was small, not well outward, in length andsmaller curved foreword length and horizontal inorientation. Horns were shaped with white patches. Ears were in smaller was prominent. Forehead was convex, wedge with long hairs. incows. was small Dewlap Poll Hump and covered cervico-thoracic was specific, Skin was grey. Muzzle and eyelids were black. with white spots (13), brown (11)and black (10). patterns, brown with white spots (34),black colourThe body varies indifferent colour and multiplication of elite germplasm. recordingin which milk is going on for selection conservation program on Siricattle under field state the in view government has started a during years the 2007 to 2012. Keeping this has shown drastic decline from 61687to 13948 indigenous cattle were Siri. cattle Siri population indigenous. It that state in the was observed all 2012 including 126519 crossbred and 13948 had 140467cattleSikkim year heads inthe providing earnings to farmers. good the soyabean. Large cardamom (spices) farming is rice, maize, finger millet, pulses, mustard, and lacking. Major crops grown area in the included available only inremote areas where AIfacility is jk-i-vk-la-C;wjks

15 ANNUAL REPORT 2014-15 Phenotypic Characterization 16 ANNUAL REPORT 2014-15 Phenotypic Characterization 75.56±2.29 cm and 95.56±6.59 cm, respectively. cm, 18.24±0.2640.88±0.7520.84+0.37, cm, 157.80±2.52,162.16±2.6116.48±0.79 in cows (25)were 106.32±1.40cm,114.20±1.55 width, length without tail switch and with switch horn length, ear length, face length,girth, face length, height at wither, paunch girth, heart according to age and The average sex. body different theagedata All sex. and were analyzed measurements were recorded on 68animals of to 600days, respectively. The ten differentbody ranged from 40-60months, 200-240days, 450 firstcalving,lactation length andcalving interval 4 months inayear for draftThe agepurpose. at with cylindrical type of type body.with cylindrical colour varied Body cattleAnimals of were Ladakhi in size small morophometric measurements, was collected. adapted altitude. to high Data on physical traits, status, management and cattle utility of Ladakhi farmers were to know interviewed habitat, the conducted region to of and 11villages Ladakh under report, visit period the During was cattle adapted to altitude high Ladakhi jk-i-vk-la-C;wjks Typical cattle Ladakhi NBAGR Resear ch Accomplishents 88.95±2.03 cm,respectively. 36.43±0.45 cm,14.36+0.19, 63.26±1.53 cmand cm, 15.08±1.069.8±0.25,14.25±0.33 switch incows were 86.22±1.44cm,93.24±0.88 face width, length tail without switch and with length, horn width, ear length, facelength, average length, height body at wither, horn Temperament of animal the The was docile. almost to ground with black and brown switch. were not prominent. Tail was long, touching was small, not well developed and veins milk in length and horizontal inorientation. Udder short and concave. Ears were to moderate small dewlap were small. Head was small. Face was forward orientation with pointed tips. Hump and Horns with upward, were curved outward then from black, brown and black with white patches. migration was reported from November to areFlocks from grazed 8-9 am to 6-7 PM. Flock enclosures with or without a thatched shed. Mouli and Yalaga flocks are in housed open isolated and purified. sheep were at collected random. DNA has been from 25 and 33animals of Mouli and Yalaga for two the sheep populations. samples Blood practices, breeding, lambing and of sale lambs on household, livestock holding, management of Yalaga sheep. Information were collected of Mouli and 16rams, and 99ewes 44lambs were recorded on 10rams, 74ewes and 32lambs covered. and weight Data biometry on body body Agasnakoppa of villages were Bagalkot district in Mustigeri, Kerur, Aminagad, Madapur and ofvillages Bijapur and district, 16flocks of Yalaga Todagundi, Nagathana, Managuli and Takkalaki of Mouli12 flocks sheep inKaladadoddi, populations of Karnataka state. survey During for characterization of Mouli and Yalaga sheep inSeptember 2014 was undertaken A survey Mouli and Yalaga sheep Extremities, except faceand ears, are white. neck. Hooves and horns are inmales brown. black. Black or brown colour may extend upto or brown patches sizeto of complete varying region. thigh the Face is white, white with black consisted hair. thick small Ahair tuft is present in and 60.2±2.49 kg in rams. White coat colour kg inewes and 81.9±1.39cm,85.1±0.98cm 71.4±0.32 cm,74.2±0.28cmand 33.0±0.45 length height at wither and weights body were Yalaga sheep are medium sized. Average body to 24months. and age at firstlambing ewes in rangedfrom 18 are sexes the Age polled. at first breeding inrams is typical roman or comparatively straight. Both A few flocks type. were typical Noseline Madgyal are brown or light gray. Muzzle is pink or brown. some of sheep the were brown coloured. Hooves either flocks, the Kenguri sheep were present or brown ring is present around eyes. In the some of white with or without brown spots/patches. A cm and 60.9±4.79 kg in rams. Coat colour is ewes, and 87.4±2.36cm,88.3±1.4291.9±1.98 79.6±0.41 cm,81.7±0.46and 40.5±0.86kgin andchest weights body girth were 77.6±0.52cm, long legs.Average length, height body at wither, variations. and Animals are body with deep tall Mouli sheep are large phenotypic with wide sized for Rs.3500-4000inYalaga sheep. 3 months of age for Rs 4000-5000 in Mouli and FMD. Surplus lambs male are marketed at about March. Sheep are vaccinated against ET, PPR and Resear NBAGR ch Accomplishents Mouli ram well developed. Tail inmales. curved and is small Udder thin. is females Horns polled. are thick, corrugated and present animals. the Males inall are horned and Muzzle is black brown or pink. Wattles are face and ear inChitti Kajali is spread over with or dark brown faceand ear. The colour the of White (Chitti) Kajali -with and white body black 41.57 %white from (varied tail 6to 55cm),and 2. complete black or black- brown with about body basis of colour 1.Black viz., (Kali) Kajali- with colour variants distinguished primarily on the Kajali sheep inPunjab (India) / has two types ± 0.54,22.960.54and 30.50±1.44respectively. female lams were 3.62±0.52,9.720.83,17.56 1.80, respectively and corresponding figures in ± 0.57,19.600.99,26.471.56and 33.58± 1-3, 3-6and 6-12months were 4.30±0.74,9.37 of lambs male age in the groups of 0-1, birth, weightwidth was 10.85± 0.19 (47) cm. Body (kg) diameter of at tail was 14.80 (5) cm and base ear and 55.83±0.37cmrespectively (Table 1).The 0 .28,73.36±0.20,84.230.27,21.330.08 ear length and length tail weregirth, 73.97± females. The length,body height, overall chest from 30 to 76 andkg in males 26 to 67 kg in and respectively 43.23±0.36kg, varies which weight of and males females were 56.98±1.02 and adjoining of districts Punjab. The Adultbody distributed in Sangrur, Ludhiana, Barnala, Moga The Kajali is a mutton sheep is which type Kajali sheep Yalaga ram jk-i-vk-la-C;wjks

17 ANNUAL REPORT 2014-15 Phenotypic Characterization 18 ANNUAL REPORT 2014-15 Phenotypic Characterization rearing was 1.98.The 84.13%fameres rearing %. The average family members involved in sheep farmers reported that twining from varied 5to 10 maturity at about 10to 12months. Majority of andthrough females natural show service sexual some horn males were noticed. also Breeding is lambs.sexesboth The arehoweverpolled in comprising 1.97male, 36.06 female and 12.91 and 5.51other sheep. The Kajali sheep flocks size was 56.45.The flocks consist of50.94 Kajali was off slightly convex type. The averageflock animals 64.57%were of Roman and nose rest and long touching tail to ground. Out of total having Roman nose, long and pendulous ears are found large to be insizewith well-built body of white Kajali (chitti Kajali) The type. animals % animals are of Kali Kajali and 45.01%were face and ear.Out of 54.99 total animals surveyed degree andvarying pattern evenup to 95%of F M Range Female Male Sex Overall Traits jk-i-vk-la-C;wjks Table: (cm)of weight adultKajali (kg)and biometry Body sheep 26-67 30-76 (399) ±0.36 43.23 (87) 56.98±1.02 ** (486) 45.69±0.42 Weight Body Chitti Kajali NBAGR 58-86 66-98 (404) ± 0.71 72.69 (87) ± 0.71 79.92 ** (491) ± 0.28 73.97 Length Body Resear 62-88 69-90 (404) ± 0.17 72.18 (87) ± 0.51 78.84 ** (491) ±0.20 73.36 Height 68-103 76-103 (404) ± 0.27 83.02 (87) ±058 89.89 ** (491) ±0.27 84.23 Girth Chest ch Accomplishents 58-107 76-112 (404) ± 0.75 84.62 (87) ± 0.75 91.34 ** (491) ±0.35 85.81 Girth Paunch proper utilization. registration and improvement programme for its from other sheep breeds of region the and needs that Kajali the sheep is phenotypically different to 12months and The 25-30kg. results reveal Age and weight at infemales puberty were 10 800 to Thelambing 1000g. %was 80 to 90%. %) reported average greasy wool production – September. The majority of farmers (64.44 monthin the of February- March and August The sheep areperiod. generally shorn a twice year provide% also fodderto sheep during scarcity pregnantseason, ewesand to lambs. The 67.21 concentrate during to sheep breeding especially on grazing however 8.12%farmers provide (8-10 hrs). The sheep are primarily maintained sheep their fromgrazed 10or 11AMto 6PM Kajali were. The majority of farmers (56.25%) 15-27 17-26 (401) ± 0.09 21.30 (87) ± 0.20 21.47 NS (488) ±0.08 21.33 Length Ear 12-29 19-27 (404) ± 0.07 21.68 (87) ± 0.16 23.77 ** (491) ±0.07 22.05 Length Face Kalli Kajali Kalli 8-14 9-16 (404) ± 0.07 10.65 (87) ±0.16 12.07 ** (491) ±0.07 10.90 Width Face 31-80 35-79 (403) ±0.37 54.78 (87) ±1.02 60.71 ** (490) ±0.37 55.83 Length Tail in distinct groups. Principal Component Discriminant Analysisthe classified five ecotypes component analysis. Morphometric traits based classical discriminant analysis and principal traits of five the ecotypes were analyzed using associated to Data ecotype. the on morphometric cultural functions and rituals socio-cultural value, traditional association to sheep, the socio- growth, meat quality, fertility, prolificacy, market level of crossbreeding, maintenance of pure stock, on factors- the based onsheep ecotype ascale 21-40. The shepherds were asked theto rank number of shepherds ranged interviewed from fromcollected distribution their areas. The merits of fiveecotypes Deccani of sheep were reproduction status; threat status and breed data on production survey Interview based and sheep of Deccani Ecotypes Resear NBAGR ch Accomplishents Principal Component Analysis of Deccani ecotypes eliminated. weightbody as multicollinearity problem was correlatedoriginal variables inpredicting the models were more appropriate of than use the The principal component regression based and traits identified with greater variability. morphologicalthe structure of ecotypes the favour of shape. body PCAwas able to define size, whereasbody second factor in was loaded loadings for had high case variables related to degree of factor loadings. The first factor each in forextracted although ecotype, each with varying component factor analysis, two factors were Inthree ecotypes. varimax the rotated principal total variance ranging from 66.3%to 71%inthe traits.two components The PCAextracted a with and Kolhapuri using ecotypes 14morphometric Analysis was on performed Solapuri Madgyal, jk-i-vk-la-C;wjks

19 ANNUAL REPORT 2014-15 Phenotypic Characterization 20 ANNUAL REPORT 2014-15 Phenotypic Characterization 49.17± animals of upto sixmonth age were 44.39±0.99, Thein males. average values forsame traits in 8.22± 49.09±0.02, 11.700.48,2.600.37,10.300.35, and 34.48± cm and 10.39± 36.48± length of three month aged animals were length, horn length, ear length and tail length, paunch chest facebody girth, girth, The mean measures for height at withers, of white or black color on body. seen their is also and grey hair on body. the Sometimes big patch White, mixture of colours these mixed with white constitute goats with coat colour brown, black, goat of Sikkim district Sikkim. germplasm concentration of goats these is more inWest goats are spread through out but Sikkim the oftract Singharey goats. Although, Singharey since ancient has which times become anative terrain and rocky mountain slopes of Sikkim goats have Himalayan inthe surviving been AsSikkim. informed people by these local the of Himalayan region and adjoining the areas of to south. the Singharey is atypical native goat northeast; and, in India, by West state Bengal Autonomous Region of to China northand the to west the and Bhutan to east; the by Tibet the independentthe Himalayan kingdoms of Nepal located eastern Himalaya. in the It is bounded by is oneSikkim of states smallest the of India and goats Sikkim 4.17± 12.13± 43.08± 13.65± measurements of animals of age 12months were and 14.08±0.71kg;respectively Body inmales. 14.33± jk-i-vk-la-C;wjks 0.29 cmand 10.21± 0.29, 11.91± 0.36, 5.58± 0.83, 53.48± 0.87, 42.83± 1.50, 47.58± 0.29, 1.95± 0.56 kgrespectively infemales and 1.76, 39.91± 0.41; respectively in females 0.57, 11.17± 0.14, 11.43± 1.15, 58.22± 0.83, 47.48± 1.31, 54.58± 0.23, 9.35± NBAGR 0.57 kg;respectively 1.31, 45.09± 0.58, 8.50± 1.98, 13.78± 1.25, 51.96± 1.76, 60.67± Resear 0.37, 8.83± 0.25 cmand 0.51cm 0.51cm 1.41, 0.34, 0.34, 1.51, 1.51, 2.43, 2.43, 0.29 ch Accomplishents 48.55± 46.71± cm &17.17±0.50kgrespectively infemales and 14.97±0.23, 5.870.41,12.550.34,10.160.38 51.39± animals having age more than 18months were averages for different biometrictraits of adult cm & 18.76±0.95 kg respectively The in males. 15.33±0.39, 8.620.58,12.000.35,10.100.37 55.86± cm &28.07±0.45kgrespectively infemales and 16.71±0.14, 9.560.30,12.920.16,10.550.16 teats are moderately developed. aroundring was observed neck. the Udder and of goats. these In breeding bucks, ablack colour black or white. Black top in many line was seen brown or white. are Legs short, stout, medially and backward.belly The under is generally light but base the pointed at tip, the orienting upward The few cases horns are strong,flat, thicker at half. Rudimentary ears in were observed also ears have black or white margin on apical the medium pendulous semi with round tip. The ishead medium inlength.ears The are short to eyes The stripes. facial are bright and small. The distinguished from other populations by the of goat Sikkim the population. can They be Singharey goats constitute major the part cm &32.31±0.76kgrespectively inmales. 17.48±0.16, 14.550.44,13.120.18,11.330.19 0.99, 52.52± 0.87, 50.57± 0.56, 59.47± 0.69, 61.94± Facial features of Singharey goat 0.90, 57.81± 0.88, 61.71± 0.47, 69.41± 0.69, 72.96± 0.86, 63.42± 1.86, 66.90± 0.48, 77.76± 0.70, 78.29± 1.46, 1.46, 1.82, 1.82, 0.83, 0.83, 0.91, 0.91, and also from Black Bengal in their body size.and from body intheir also Black Bengal ofrespect coat colour, of type horns and size their These goats arefrom distinct Singharey goats in were more seen of northdistrict inthe Sikkim. BlackSikkim goats with black jet uniform colour 33.86± & 9.00± 51.00±2.08, 12.070.18,2.0011.210.46 were 36.57± and length tail of three month aged animals face length, horn length, ear lengthgirth, at withers, paunch length, chest body girth, The means with stan Resear same traits inanimals of upto sixmonth age were respectively in The males. average values for 11.86±0.63, 2.46±0.42, 10.43 ±0.29 & ±0.40 8.64 ADULT MONTH UP TO 12 MONTH 6 UP TO MONTH 3 GROUP AGE 2.23, 41.14± NBAGR 0.38 cm;respectively in females and M F M F M F M F SEX 1.11, 43.07± 64 56 13 15 06 16 16 15 N ch Accomplishents Singharey Buck Singharey 55.67±0.93 52.52±0.71 47.15±1.01 50.93±1.02 41.33±2.23 45.81±1.02 33.86±2.23 36.57±1.11 Height Body Table :Average measurement body of Singharey goats 1.28, 46.00± dard errors for height 61.48±0.86 60.29±0.66 50.00±1.22 53.12±1.47 46.33±1.67 49.94±1.05 41.14±1.28 43.07±1.00 Length Body 1.00, 47.21± 1.54, 51.21± 71.66±0.85 67.98±0.59 62.15±2.45 57.87±1.31 53.33±2.79 52.69±1.47 46.00±1.54 47.21±1.58 Girth Chest 1.58, 2.72, 2.72, 76.47±1.16 74.52±1.25 66.00±2.00 64.07±1.75 57.83±4.44 55.81±2.32 51.21±2.72 51.00±2.08 Girth Paunch 45.81± &10.00 64.07±1.75, 14.930.30,6.530.67,12.670.61 were 50.93± measurementsBody of animals of age 12months 11.367± 53.33±2.79, 57.834.44,14.000.37,4.830.91, in females and 41.33± 13.69± 50.00± 55.67± &10.88 74.52±1.25, 16.160.19,8.840.33,13.480.18 were 52.52± adult animals having age more than 18 months The averages for different biometric traits of 8.38 adult females and (ta 31.03±0.92inmales increasing with age and were 27.33±0.65kgin respectively body weights The inmales. were 17.20± 17.20±0.22 16.16±0.19 15.62±0.49 14.93±0.30 14.00±0.37 13.69±0.46 11.86±0.63 12.07±0.18 Length Face ± 0.64, 11.85 ± 0.22, 14.58± 0.46, 4.56± 1.02, 49.94± 1.22, 62.15± 0.93, 61.48± ± ± 1.05, 8.17± 0.24 respectively in females and 0.43 in females and 47.15 14.58±0.57 8.84±0.33 8.38±0.64 6.53±0.67 4.83±0.91 4.56±0.34 2.46±0.42 2.00±0.18 Length Horn A flock of Sikkim Black goats 1.02, 53.12± 0.71, 60.29± 0.34, 11.94± 0.57, 12.92± 1.05, 52.69± 2.45, 66.00± 0.86, 71.66± 0.50 & 9.85 0.75, 12.83± 12.92±0.24 13.48±0.18 11.85±0.50 12.67±0.61 11.367±1.05 11.94±0.25 10.43±0.29 11.21±0.46 Length Ear 2.23, 46.33± 1.47, 57.87± 0.66, 67.98± 0.25, &13.88± 11.89±0.25 10.88±0.24 9.85±0.36 10.00±0.43 8.17±0.75 9.56±0.27 8.64±0.40 9.00±0.38 Length Tail 0.24 &11.89± 1.47, 55.81± 2.00, 15.62± 0.85, 76.47± ± 0.36 in males. 1.01 in males. jk-i-vk-la-C;wjks 31.03±0.92 27.33±0.65 18.62±1.17 16.80±0.73 12.83±1.01 13.88±0.75 10.31±0.91 10.53±0.62 Weight Body ± ble 4). 1.67, 1.01, 1.31, 0.59, 2.32, 2.32, 0.49, 0.49, 1.16, 1.16, 0.75 0.75 0.25 0.25

21 ANNUAL REPORT 2014-15 Phenotypic Characterization 22 ANNUAL REPORT 2014-15 Phenotypic Characterization the mangersthe are made on also ou the providealso ventilation. good In some houses, not accumulate an floor. the flooring The raised housethe neat and droppings clean the as all do ground in many Thistype of cases. flooring keeps The floor ofgoat house were from raised the proper arrangement and of water electric supply. made of sticks bamboo and logswith no wooden kept inatemporary structure. Goat houses are eveningthe by 6.00p.m. In night the are they forests morning in the and are brought back in for meat The purpose. animals aretaken to hill andsmall marginal farmers of mainly Sikkim maintained on semi-extensive management by breedingthe indicated tract that goats these are The preliminary information recorded from for 34.87±1.56kg. males weight for adult females was 28.48±0.72 kg and 13.50±0.36 and 12.20±0.40cm.The average body 75.97±1.48, 82.30±1.76,17.90±0.29,15.070±.77, measurements were 54.70±1.19, 61.80±1.39, cm; respectively, whereas for average the males 17.11±0.20, 10.17±0.53,12.70±0.23,10.35±0.24 50.06±0.85, 58.75±0.73,70.65±0.73,79.57±1.17, length and length tail inadult female goats were paunch face length, horngirth, length, girth, ear goats for height at withers, length, Chest body sexes.The average measurements Black of Sikkim in few animals is Beard seen but present in both is black, hooves are grey. Underbelly black. is also at pointed base, at tip, backward. curving Muzzle flat. The horns strong,are in colour,grey broader are slightly shorter than Singharey goats. Nose is longer than that of Singharey goats. These goats proportionate to body. Face, horn and are tail Sikkim Black Sikkim jk-i-vk-la-C;wjks Housing pattern for Sikkim goats goat s are of medium size. Head is NBAGR Resear ter wall. ch Accomplishents natural mating. common goats.very inthese Breeding is through ranges 145-155days.period between Twinning is 9-12months. varies between also The gestation months). The age of maturity in female goats are males the cases, castrated at younger age (3 at age the serve of 9-10months. In majority of The male goats mature becomesand able to on regular is left basis. The milk kids for suckling. day. However, goat the milking is not practiced production is approximately per 300–500ml udder but conical withshaped small teats. Milk Singharey goats have moderately developed maizecrushed and tree leaves. jungle.the fed Stall goats are kept grass, on local Goats are kept vegetation on local available in also been recorded. been also Aone day workshop on Phenotypic characters and weights body have fromcollected 366farmers through interview. mortality, reproduction and production has been flock structure and size, management practices, Imphal West of districts Manipur. Information on conducted has been Survey inThoubal and Kaunayen of chicken Manipur Stall feeding of Sikkim goats named as ‘Kaunayen’ instead of Coman. indigenoustype chicken of Manipur should be Manipur. The breeders thatsuggested fighter and documenting indigenous chicken of and information the required for characterising apprise about them importance the of project the to generate awareness among breeders and Imphal incollaboration (W) with CAU, Imphal organized on 21.11.2014at Keishamthong, Characterization of Manipuri chicken was Resear 76.88±2.35 respectively. and haugh units were 0.07±0.01, 0.38±0.01, spots were absent. Albumen index, yolk index present inabout 14percent meat of while eggs shell (including membranes).Blood spots were percent albumen, 37percent yolk and 12percent Onaneggs. average, an was composed egg off 51 Albumen was thick inmore than 85percent of 85 percent and yellow deep in 15 percent of eggs. 0.36±0.01mm. Yolk was yellow incolour inabout was quite strong having average thickness of of Shell 42.43±.07g. colour was brown. Shell Eggs were medium in size with an average compared to Aseel. adapted and can fight for longer duration as but breeders the informed that are these better similar to other such birds like Aseel, etc. Danki, annum. These are fighting type birdslook and 2.5 to Egg production 3kg. is about 35to 45per in colour weight and is Body about is type. of pea of white, black or golden feathers. Comb is red Plumage colour is black or brown with patches NBAGR Kaunayen chicken of Manipur of chicken Kaunayen ch Accomplishents The age at first whelpingabout is 20 months. duration ranges of oestrus 12to 25days. between maturity at an age of about 12-14months and 20.52±0.35 kg(n=128).The bitches attainsexual weightbody of adult Chippiparai animals is kaathu ears aretype as Kutthu called dropping (47%)and (2%).The erect erect type is medium and flat,with droppingsemi (52%), sometimes extending up to head.ear The size around lower particularly eyes the eyelids, with other coat colour there are black circles canthus of of eyeswhereas the incase animals have white markings on sidesabove both inner The withasanimals Sevalai. dorsal black coat with brown or brownish black coat colour called coat colour as Kanni called and 10%of animals as Pullai, 20%ofis called animals with black animals are with fawn coat colour (70%)which brownish black and black. Majority of the coat colors from varying fawn to dark brown, Chippiparai dogsare medium insizewith as Kannicalled or vettai naai (hunting dog). in Madurai Chippiparai district. dogs are also nameChippiparai came aftercalled thevillage ofdistricts southern Tamil Nadu. Chippiparai Madurai, Virudhunagar and Thoothukudi Chippiparai dog is distributed in Tirunelveli, dog Chippiparai Chippiparai dog Chippiparai jk-i-vk-la-C;wjks . The adult

23 ANNUAL REPORT 2014-15 Phenotypic Characterization 24 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics Genetic Characterization& owners.their by breeders the as hobby their and as apride of for guarding and hunting. These animals are kept mainly with non-vegetarian and food utilized 12-16 months. The dogs arebeing maintained The age maturitysexual at indogsrangesfrom was generated and on analysed. different Based (Gir, and Sahiwal Tharparkar) using 4 STR loci Thedata on 541farm individualsfrom 3 breeds cattle Tharparkar Breed signature for Gir and Sahiwal, average inbreeding coefficient (F significantly different from zero (P<0.05). The F and other cattle breeds, various estimates for assess overall the genetic structure of Ladakhi cattle sufficient revealed genetic variability. To heterozygosityand expected (0.79)for Ladakhi heterozygosity (4.84),observed alleles (0.75) number of (9.95),effective alleles number of breed diversity measures interms of observed The various locus. of within per 9.95alleles acrossdetected with mean 20loci number the heterozygosity values. A total of were 200alleles genetic variability interms of diversity allelic and across 20markers was analysed to estimate the Microsatellite genotypic based data recorded Cattle Ladakhi between differentbetween cattle breeds. depicted by F and other cattle Ladakhi populationsbetween cattle was 0.037.The interbreed differentiation Functional Genomics -statistics were calculated. The values were jk-i-vk-la-C;wjks ST reflected high reflected high divergencegenetic NBAGR Resear IS ) in Ladakhi ) inLadakhi

ch Accomplishents assignment. for Sahiwal, Gir and Tharparkarcattle breeds; The kit was tested and validated used to be overcome identification of overlappingalleles. were loci These taggedwith different dyes to have same melting and annealing temperature. microsatellitekit. the All were loci to modified Eight were loci to develop used amultiplex PCR three the breedsbetween studied. samples a great revealed amount of gene flow three breedsthese was less precise. The field However assignment the ofsamplesfield for assigned and 3breeds formed different clusters. individuals from organized farms were correctly individuals to different populations. the All The 'Structure'software run tothe was assign assignment was down to 93%. addingsamples field the for all thebreeds correct 25% for 11and respectively. 8loci However after amongrevealed population variance of 21and of breeds. The Analysis of Molecular Variance the three breedsthe The with F 11loci. correct assignment achieved could for be all each breed were identified. Hundredpercent to three populations. The top ranking loci for of were loci for used assigning individuals the heterozygosityalleles, etc. different combinations criteria for of like Fst, selection loci Numbers of The F The to assign 100%individuals of three breeds. these was attemptedloci and were finally8loci able Tharparkar, respectively. selection ofFurther Sahiwal, Gir and Tharparkar and Sahiwal and were 0.173,0.281and Gir 0.235between and ST values further increased between pair increased between values further ST values Resear Farm and Field Animals Farm Animals Individual NBAGR Within 66% AMOVA with11 Loci ch Accomplishents Structure resultsStructure for breed assignment in cattle (K=2,3and 4)using 8loci Analysis of molecular variance using 11and 8loci Populations Among 21% Individuals Among 13% Individual Within 61% AMOVA with8Loci Populations Among Individuals 25% Among jk-i-vk-la-C;wjks 14%

25 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics 26 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics cytogenetic constitutioncytogenetic with 2nchromosomes and Paralakhemundi having typical riverine buffalo Cytogenetic studies have shown Kalahandi both and Gajapati breeding inthe districts tracts. urban and areas peri-urban of Kalahandi both AI using Murrah in is practiced semen being appearance. animals havingsimilaritiesinphenotypic having anoverlappingbreedingtractwith and Paralakhemundi buffaloes to be Preliminary surveyshowsbothKalahandi traits oflocal buffaloes also collected. including productionandreproduction socio-economic and management practices further geneticanalysis.Informationon for karyotypingand DNA isolation and tract, breeding buffaloes ofOdishainthe representing Kalahandi and Paralakhemundi Blood samples werecollected from animals Odisha buffaloes jk-i-vk-la-C;wjks Mitochondrial diversity phylogenetic haplotypes analysis based and of riverine swamp buffaloes. NBAGR Resear ch Accomplishents riverine buffalo breeds. Odisha buffalopopulations grouping with other on haplotypes sharing, shown has also the both haplotypes. Median joining network analysis based 81 variable sites resulting were observed in 57 across nine riverine and swamp breeds/populations, sequence data generated. By comparative analysis and 19Paralakhemundi buffaloes amplified and bp mitochondrial D-loop region of 30Kalahandi number of 50.Primers have and designed 811 been Typical of female karyotype riverine Paralakhemundi buffalo were monomorphic. found polymorphic, to other be regions while RFLP technique. Only Exon 40of FASN gene was variation in Chhattisgarh buffalo, using PCR- 42) and 3’ UTR of STAT1 were analysed for also production traits -FASN (Exon 38,39,40,41, (N=50). Thecandidate genes affecting the fat withriverine chromosomal type number fifty Chhattisgarh buffalosamples beit revealed to farmers’ Cytogenetic interviews. analysis of through buffaloes wereof collected also local including production and reproduction traits Socio-economic and management practices 140 adult Chhattisgarhi buffalo were recorded. done and biometric measurements on about Chhattisgarh. Phenotypic characterization was block to explore buffalo the genetic resource of including Lohandi Guda block and Kondagao Kawarda and of Baster districts Chhattisgarh of Dhamtari, Kanker, Mahasumund, Bilaspur, werePilot conducted surveys inabout 41villages Chhattisgarh buffalo Resear Odisha and other riverine buffaloes NBAGR Median joining analysis network of buffaloes Odisha on based mitochondrial haplotype ch Accomplishents sharing with other riverine and swamp buffaloes riverine other with sharing animals having different haplotypesDQA of PCR-RFLP analysis, PCR samples from eight for HinfI and HaeIII, respectively. on Based geneDQB had sixand restriction patterns seven atotalrevealed of patterns, allelic seven whereas RFLP analysis of DQA gene for enzymes the both HaeIII and HinfI restriction enzymes. PCR- products of genes the both were digested with were amplified in 90swamp buffaloes. Amplified genes,DQB respectively encompassing exon 2, bp genomic regions corresponding to DQA and diversity at MHC class about II loci, 930and 290 populations from NE region. To assess genetic wereloci swamp studied inlocal buffalo Complex (MHC), agroup of immune response swamp Major buffaloes, Histocompatibility to assess genetic the diversity and fitness of reared mainly for meat and draft. As an attempt bovineto specific North-East region of India is carabanesis bubalis Swamp buffalo (Bubalus inswamp buffalo loci DQB diversityGenetic at MHC-DQA and Swamp buffaloes jk-i-vk-la-C;wjks ), a

27 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics 28 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics population under being positive selection. swamp buffaloes, indicating higher fitness the of duplication of DQA inindigenous and loci DQB diversitythus allelic high revealed as well as were at seen binding peptide the sites. Results Moreover, most of amino the acidvariations duplicationthe genes of inswamp DQ buffaloes. DQA and from DQB an individual confirmed presence of more than two different clones of diversity inIndian swamp buffaloes. Further, indicated positive for selection MHC class II of dN/dSfor DQA overall, and alleles DQB and HighDQB2 DQB3. ratio (more than 1) corresponding to three major groups i.e. DQB1, Foralleles). DQB, total were 16 alleles identified major groups, DQA1 and (11 alleles) DQA2 (2 swampwhich corresponded buffaloes, twoto A total of 13 DQA were alleles identified in clonesof selected was isolated and sequenced. clones having differentalleles and plasmid DNA Colony PCR-RFLP out was carried to the identify and genes DQB were cloned, for studies. further of HSP90AA1 containing 7already reported an amplicon size of 258 bp. Promoter region size of 354bp. exon HSP90AA1 partial 8with of 490bp and complete exon 9with an amplicon 6- exon 7 complete exons with an amplicon size successfully amplified included, HSP90AA1 exon The fragments of HSP90 genes, were which by specific and allele tetra-primer ARMSPCR. and HSP90 genes and identification the of SNPs amplificationthe of different regions of HSP70 differentThirteen setsprimer were designed for Magra=54 and Madras were Red=42) isolated. different sheep breeds (Chokla=47, Marwari=52, were studied. Atotal of 195DNA samples of 4 genes infour different breeds of Indian sheep SNP markers heat inthe shock protein (HSP) Indian sheep Heat stress protein genes in jk-i-vk-la-C;wjks NBAGR Resear ch Accomplishents Magra than and Marwari breed Chokla of sheep. Hb%, PCV% and TEC were found higher in to be temperature,the respiratory rate, pulse rate, 8.79, 9.93; respectively. The average values for 9.58, 9.62,10.64;33.97,34.58,39.82and 8.27, Marwari and Magra breed were found to be The Hb%,PCV% and TECvalues Chokla,of breeds of sheep the under unstressed condition. parameters were measured for three different physio-biochemical and haematological werealleles 0.60and 0.39;respectively. Different respectively.frequencies allele The of G and C genotypes had of afrequency 0.40,0.40and 0.19; Sheep were genotyped, where GG,GCand CC specific samplesprimers. 42 the of MadrasRed 112 nt position SNP (G/C) using allele the Genotyping protocol was for developed the promoter region of HSP90AA1. developed for -660and -601nt position inthe The tetra-primer ARMSPCRprotocol was SNPs at 112,244and 248nucleotides position. 6 samples of fragment this three novel revealed amplified.successfully DNAsequence analysis of SNPs with an amplicon sizeof 499bp was also (Riverine buffaloes) were collected at different Friesian cows (exotic cattle) and Murrah buffaloes cattle), Karan Fries cows (Cross-bred), Holstein healthy animals of cows Sahiwal (Indian native cattle andsamples types buffaloes. Milk from 200 duringmilk course the of lactation in different capacity and scavenging radical free activity of comparativethe changes intotal antioxidant mechanism. The aim this of studyevaluate was to help to activate antioxidant body the defense production of reactive oxygen and species constituents, are which to prevent crucial the enzymatic and non-enzymatic antioxidants animals fromMilk contains dairy several milk scavenging of activity bovine Antioxidant radical and capacity free its products by affecting flavour,its texture and play physiologically significant rolesand in milk Proteases are important class of enzymes that somaticderived cells of Expression proteases kinetics inmilk youngthe or calves. born new additional antioxidants to supply immunity to produced throughout early lactation stages has ofpart and colostrum the lactation. milk So and early lactation stages as compared to later stages of lactation and are incolostrum higher that antioxidant levels change during different significantlylate in lactation. These suggestdata and midlactationpeak stages, but it decreased significant differences were found in early, mid and late lactation stages. In HFcows, no and early lactation stages compared to peak, of DPPH was significantly incolostrum higher Murrahpercentage buffaloes, scavenging activity andin Sahiwal Karan Fries cows. Whereas, in showed no difference across lactation stages side, percentage scavenging activity of DPPH across differentlactation stages.the On other no significantbuffaloes, differenceobserved was and late lactation stages, whereas inMurrah was significantlyearly in higher stagethan mid stages. Also, inHolstein Friesian cows, TAC followed by early, midand late peak, lactation was significantly incolostrum (p<0.05)higher In and Sahiwal Karan Fries cows, TAC of milk comparison and midlactation to peak periods. duringhigher and colostrum early lactation in TACradicals. to be was observed in milk using 1,1-diphenyl-2-picrylhydrazyl (DPPH) scavenging radical free activity was evaluated reducing/antioxidant power assay and (FRAP) capacity (TAC) of was measured milk by ferric and late lactation (>215days). Total antioxidant (30-60days),days), mid(100-140days) peak lactation stages; early colostrum, lactation (5-15 Resear NBAGR ch Accomplishents buffaloes, buffaloes, Karan cowsFries and Holstein Friesian cows. Scavenging activity measured by DPPH assay during different stages lactationof cows,of Sahiwal Murrah jk-i-vk-la-C;wjks

29 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics 30 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics of different classes of proteases (cathepsins, conducted to assess relative the mRNA levels are poorly understood. The present study was expressionthe associated with kinetics them proteasesthese among different and species on comparative the expression profiling of date no significant studies have conductedbeen across different stages lactation.of However till proteases varies from as well to species as species longevity. The expression endogenousthese of Relative mRNA level of cathespsin protease genes inMurrahsomatic buffalo milk cells during different lactationstages. Values are expressed as means± SE. Different letters indicate significant differenceslactationbetween stages (p<0.05) jk-i-vk-la-C;wjks NBAGR Resear ch Accomplishents of derived somatic milk cells cells (MSC), the mid (90-120days) and late (>240).After isolation of early lactation (30-60), viz. (5-25days), peak animals eachof 4stages) during different stages each of cows Sahiwal and Murrah (5 buffaloes were from collected 40animals including 20 buffaloes. Forthis study,freshsamples milk lactation stages in Sahiwal cows and Murrah pathways associated genes) across various plasminogen and ubiquitous-proteosome were comparatively inbuffaloes. higher however; expression the species the both levels pattern of studied proteases genes was similar in trendearly lactation. Theof expression observed expression during late lactation as compared to plasminogen pathways. had increased also They activator inhibitor) genes associated with analyzed B1and 1-(Plasminogen Serpine Serpine especially, cathepsins. the Further, we also highest expression level for most of proteases the While cells the from late lactation stage showed of different proteases during early lactation. lactation. analysis Our showed lower expression an increase inexpression during course of encoding different endogenous proteases showed cows and Murrah genes the All buffaloes. MSC during differentlactation stages of Sahiwal (SERPIN SERPIN E2 and F2) were evaluated in and Atrophin 1 ( (PLAU), Urokinase(CTSZ), plasminogen type activator S has been speculated to be a good alternative agood to speculated be has been to cows.Sahiwal Use of MECisolated from milk and fatty acidsynthesis genes inMECof expression pattern of important caseins milk therefore aimed to determine relative mRNA of Indian native cow. The present study was epithelial derived mammary milk cells (MEC) and fat related genes gland or inmammary about expression the profile of major milk At present not much information is available cows ofepithelial cells Sahiwal and fat genes metabolism inmammary Expression profiling caseins of milk H B (CTSB), of different classes of proteases; Cathepsin analysis. Comparative expression patterns were subjected to RNA isolation and expression Resear (CTSL), Cathepsin L(CTSL), (CTSS), (CTSH), Cathepsin (CTSH), K NBAGR Tissue plasminogen activator (PLAT) Cathepsin Cathepsin D(CTSD), ATRO1) and protease inhibitors ch Accomplishents (CTSK), Cathepsin (CTSK), Cathepsin Z

rel expression pattern of important and milk fat provide understanding the of stage specific mid and late These results lactation periods. in early lactation (10to 30days) compared to FABP3 GPAM and (>240 days). Similarly, expression level of SCD, and inlate gradually decreased lactation stages was significantly during high early lactation late lactation. Their mRNA abundance level as compared lactation period peak to midand genesof casein wasduring higher all early and and analysis. BestKeeper Expression pattern were identifiedthrough geNorm, NormFinder dehydrogenase (ACTB) (GAPDH), actin-beta protein L4(RPL4), glyceraldehyde-3-phosphate elongation factor 1alpha (EEF1A1),ribosomal four stable best HKGs; translation eukaryotic classes werefunctional evaluated. Apanel of housekeeping genes (HKGs) from different For normalization of expression data, 10known BTN1A1, ( genescasein (CSN1S1, CSN1S2, CSN2, CSN3) analysis. The expressionpatterns the milkof lactation stages and subjected for expression of 52MECsamples werefrom purified different and stored processing. at further Atotal 4°Ctill AM.) and brought to laboratory insterilejars earlycollected morning 5:00-6:00 (between liters animal) from animals per were selected mid (90-120)and late samples (>240).Milk (2-3 stages of lactation: early (30-50), (0-20), peak multiparous cows Sahiwal during different samplesmilk were from collected 16healthy coated with anticytokeratin 18antibodies. The were isolated from Beads fresh using milk, Dyna In present the difficult. investigation, MECs animals where obtaining biopsies is sometimes largeassociated with lactation, inparticular dairy expressionthe profile of important genes gland tissuesmammary inorder to understand LALBA), and fat metabolism genes (FABP3, ated gen ACACA, SCD, GPAM) were evaluated. es in mammary epitheliales in mammary cells of etc . was significantly higher jk-i-vk-la-C;wjks

31 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics 32 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics samples were and collected DNA was isolated PRDM9 gene in bovines was studied. Blood S sterility inbovines sub-fertility of basis male Molecular or of Indian native cows. fat-proteinmilk synthesis in gland mammary in understanding molecular the regulation of cows.Sahiwal Findings a step could be forward equence variation in zinc finger do jk-i-vk-la-C;wjks Relative mRNA level of caseinsand milk fat metabolism genes purified epithelial inmilk cells of NBAGR Sahiwal cowsSahiwal during differentdays lactation.of Resear main of ch Accomplishents was recorded infour cattle, viz. bovine species were standardized and specific amplification accession no. KJ020105).PCRconditions taurus domain of PRDM9 gene on basis the of Primers were to designed amplify zinc finger Swiss, Karan Fries, Frieswal and Jersey cross). Holstein Friesian)and crossbred cattle (Karan from indigenous cattle, exotic cattle and (Jersey gene sequence available (GenBank Bos Bos allele respectively.allele PRDM9 gene is 6, 7, 8 and 9 in A, B, C and D numberthe of zinc fingersthis domain in of and sequencing of that revealed 4alleles the have recorded Cloning been species. inthese CD,AB, BC, AC and BD) for PRDM9 gene and D)and nine genotypes (AA,BB, DD, CC, buffalo, (A,B, and alleles yak C mithun. Four Resear NBAGR ch Accomplishents Alleles and genotypes observed in PRDM9 across gene Alleles observed different and genotypes species Pictorial representationPictorial of PRDM9 in gene bovines unique or alleles combinations of Even alleles. evolvedhighly germplasm, hence might possess climatic conditions.cattle Ladakhi are These well underand at very can survive extreme overdeveloped years the under natural selection cattle region from ofLocal Ladakh India have cattle traits inLadakhi to milk profilecandidate Allelic of genes related jk-i-vk-la-C;wjks

33 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics 34 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics characteristics at most of studied (e.g., the loci cattle haveLadakhi maintained indicine the cattle breeds as compared to taurine cattle breeds. cattle was similar to other studied Indian native pattern frequency allelic inLadakhi observed across cattle. 72animals the of Overall, Ladakhi -3) and Diglycerol Acyltransferase (DGAT-1) Prolactin (PRL), Butyrophilin1, 3 (BTN-1, (bGH), Pituitary transcription factor (Pit-1), bovine Growthlactoglobulin (α-LA), Hormone (β-LG), Alpha-(β-CN), Beta-lactoglobulin including Kappa-casein (κ-CN),Beta-casein of variants allelic at important candidate genes present study distribution the assessed pattern candidatethe traits. gene affecting dairy The loci hence it is to pertinent study profile allelic the at cattle local this provides around 3-4 kg of milk, under ext with survival the (THI 85).The neutrophil/lymphocyte (N/L) atcollected temperature high humidity index cows and Murrahsamplesthe All buffaloes. were cows,Sahiwal Karan Fries cows, Holstein Friesian total of 50healthy heifer animals, 10-12each of and Murrah This buffaloes. study included a Allelic frequency distribution frequency Allelic cattle, across tropically Ladakhi adapted Indian cattle and exotic cattle at various loci jk-i-vk-la-C;wjks NBAGR reme conditions, Resear ch Accomplishents on naturally the cattle. evolveddakhi La absence of taurine influence/introgression effect The present near findingsthe highlighted also variants novel cattle were to Ladakhi observed. Indian native cattle breeds and at BTN-3 loci, κ-CN that has not reported inany been other identified variants, variant at Ewas observed cattle In is A2milk. additionLadakhi to already strongly loci casein suggestedbeta that from milk BTN1). Also, predominance the of at A2allele atallele β-CN1,at A allele β-CN2,at Aallele Msp of stress hormones inanimals causes alterations level of physiological stress. In general, release of whitecells incirculation blood indicate the and Murrah The relative buffaloes. numbers Karan Fries cows lower while cows inSahiwal ratio was Holsteinhighest in both Friesian and assess physiological the stress across cattle types ratio, is which considered measure as good to evaluation of neutrophils/lymphocytes (N/L) An attempt was made comparative to undertake as indicator of heat stress inbovines Neutrophils/Lymphocytes (N/L)ratio I- allele atI- allele at bGH, K allele DGATI, A2 exotic, crossbred cows to summer stress. havebuffaloes better cellular tolerance than provides that evidence cows Sahiwal and Murrah physiological stress. Thedata presented here appeared areliable to be indicator of levels of of lymphocytes and thus, N/Lratio ahigher such as an increase of neutrophils, a decrease and immunological response against antigens, of white cells involved blood defense inthe that stress conditions result inaredistribution studies withSeveral different have species shown hormones and physiological stress inanimals. researchers to indirectly infer increases instress of alterationsdetection these by used can be is not clear, but since it predictably, occurs in lymphocytes. The reason alterationsfor these that is an increase inneutrophils and adecrease of numbers of two oftypes; five the leukocyte Resear buffaloes buffaloes and Holstein cows across summer andwinter season (Significance at*p<0.05,**p<0.01 and ***P<0.001). Transcriptional stability of different heat shock protein genes in PBMCS corsof Sahiwal incomparison to Murrah Neutrophil/ lymphocyte (N/L)ratio across species NBAGR ch Accomplishents ( abundance of four major HSP transcripts hand, Holstein Friesian animals with higher than that of exotic Holstein cows. other Onthe for its better cellular tolerance and adaptability with change This inseason. provides evidence an significant or minimum change inexpression The HSPtranscripts cows in Sahiwal showed non- humidity index≤72). average temperatureof12±1°Cand 85) andcoldwinter (month ofDecember with ≥ index humidity temperature and 41±1°C of summer (monthofJulywithaveragetemperature study. Bloodsampleswerecollected during hot buffaloand Murrah includedforthis were 5 eachofSahiwalcow, HolsteinFriesiancow buffaloes ornot. A total of 15lactating animals, of Sahiwal,HolsteinFriesiancowsandMurrah on transcriptionofmajorchaperonesinPBMCs determine whether seasonal variation hasimpact The majorobjective ofthis study wasto protein genes incattle Transcriptional stability of heat shock all the HSPs the all together showed similar trend to climatic conditions. Pooled expression data for to environment intropical heat especially load summer indicated season lower their tolerance HSP 90, HSP 70, HSP 60, and HSP jk-i-vk-la-C;wjks 40) during

35 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics 36 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics ICG during studies in Indian cattle adapted to categoriesfunctional as suitable that could serve 10 well-known reference genes from different hypoxia genes incattle regulatory control gene to (ICG) normalize Identification of suitable internal of compared to cows. Sahiwal Higher expression significant induction in Holstein Friesian cows HSP90 genes together showed maximum and expression values for HSP70 that of individual HSP with summer the stress. production of heat shock proteins to cope up stresshigher state of animals, requiring more Pooled expression profile for HSP genes together during summer and winter (Significance at *p<0.05,**p<0.01 HSPs jk-i-vk-la-C;wjks inHolstein cows related could be to the and ***p<0.001). mRNA. The combined NBAGR , Ranking ofFig. on Ranking ICG Normfinder based analysis. HSP40 Resear , HSP60 and and ch Accomplishents >RPL4 >EEF1A1>UXT >ACTB >HPRT . Similar stability: value, genes were arranged indescending order of stepwise exclusion of gene the with highest the M basisthe of relative gene expression stability and expression stability (M)values below 0.5.On In geNorm analysis, genes the exhibited all software to find theout most appropriateICG. geNorm,utilized NormFinder and BestKeeper and study were; ACTB, B2M,GAPDH, RPS9,RPS15 internal control genes included present in the altitude hypoxic conditions. The candidate crosses and Jersey cows maintained at high normoxic condition, cows, HF and Ladakhi cows, Holstein Friesian cows maintained under animals each of cows, Sahiwal Karan Fries study included atotal of 8-12healthy heifer of cattle altitude to high stress condition. The to model understanduseful differential response mononuclearblood cells (PBMC) a could be with assumption the that circulating peripheral towards hypoxic stress. This study was planned underlyingthe alterations incellular tolerance provide comparative data baseline to understand genes in native cattle and exotic cattle would The expression studies of hypoxia responsive altitudehigh and tropical conditions were tested. RPS23, HPRT1, and UXT RPS9=RPS15>HMBS >GAPDH>B2M . The present study PBMC of tropical altitude vs.high cattle breeds. forused normalization the of expression data in and geometric means of 4ICG could these be HMBS demonstrated that RPS15,GAPDH, geNorm,Normfinder and have BestKeeper investigation, three algorithmic all methods towards expression their stability. In present the and(CV) standard deviation (SD), pointing index, lowerbestkeeper coefficient of variance of coefficient higher correlation (r)to the levels. reference genes showed consistent expression its breeding for tract supporting conservation stored in utilized been has Bank also inGene duringBank last year. Jaffarabadi semen buffalo procured and to added repository inGene and Tharparkar) and Buffalo (Toda) been have Total 7,600 frozen s Situ Ex parameters.cytogenetic buffalo bulls and 40 pigs werescreened fortheir year the During atotal of 113breeding cattle and of Government policy the as per defects of India. anddefects to keep herds the of free such genetic aimthe to check spread the of chromosomal various agencies throughout with country the screeningcytogenetic of breeding to males NBAGR is providing of consultancy service Chromosomal defects Cytogenetic for Screening on C based to calculateused gene expression variation of otherBestKeeper genes.algorithm The was slight variation ranking in the was observed geNorm and Normfinder outcome, albeit genes. There agreement was good between most stable and HPRT to geNorm, Normfinder identifiedalso RPS15 Resear RPS9, RPS15,GAPDH, HMBSexhibited are most the stable internal control genes NBAGR T Conservation values. Each of values. Each 10candidate the ch Accomplishents emen doses ofemen doses Cattle (Gaolao as least stably expressed RPS9 and and as as spermatozoa retaind potential. fertility the vitro extended hours slaughtering post of bucks. In forsperms conservation, their after even of utilizing for them of extraction epididymal of testis at low temperature indicated suitability prepareddoses using protocol. this The storage caprine epididymal and semen frozen semen protocol standardized has been for freezing yolkegg and antibiotics along with acooling An extender containing citrate buffer, sugar, caprine genetic biodiversity spermatozoa for of cryopreservation Utilization of epididymal cauda and Yak). (Cattle, Buffalo,Goat, Sheep,Camel, Equine belonging to forty four breeds of species seven NBAGR now stores 1,29,174frozen doses semen and improvement. The NationalBank Gene at respective centers. Harringhata chicken out was carried by their conservation of Bargur cattle, Ongole cattle and Rajasthan, Hazra chicken, Duck of Assam and Assam, Jalori camel, Arunachali Yak, Donkeys of Assamese cattle, Kosali Cattle, pig population of sheep, Mithun, Cattle, Purnea Binjharpuri Cattle, Goat, sheep, Marwari Poonchi Sheep, Tibetan of Budelkhandi Goat, Bhakarwal Characterization Network onAnGR Project In of goat fertilization vitro ova by epididymal sperm fertilization that revealed frozen epididymal and zygote formation jk-i-vk-la-C;wjks

37 ANNUAL REPORT 2014-15 Ex situ conservation 38 ANNUAL REPORT 2014-15 network project on angr of Bundelkhand region. The region is located in Bundelkhandi goats are found parts inalmost all goat Bundelkhandi and other nomadic communities rearing the delineated.Reasi, has Gujjars been Bhakarwal, different namely districts RajouriPoonch, and Migration route of Poonchi sheep inthree Poonchi sheep products like hair and are milk other items of utility. rearedbe for excellent quality meat and animal goats Bhakarwal aretract. primarily known to from to March October stay they native intheir goatBhakarwal remain at pastures highland and AprilDuring to September breeders the of delineated inKathua, Rajouri and districts. Reasi Migration route goat of has Bhakarwal been goat Bhakarwal Ears are medium and pendulous. and slightly outward inorientation. Tail is bushy. and strong, horns are of medium size, twisted posture.with good Chest is wide, legsare long Bundelkhandi goat of to seems be medium size concentrate ration to lactating &pregnant does. grazing alone is preferred, only few farmers offer and itmilk market. sell to local the Mostly Some goat keepers make ‘Khoya’ from goat the It goat is dual type yielding meat both and milk. environmentin fragile of Bundelkhand region. in central India. Goat-rearing is sustainable even Indo-Gangeticthe plains on Vindhya tract hilly jk-i-vk-la-C;wjks Bundelkhandi goat goat Bundelkhandi NBAGR Resear ch Accomplishents different wool parameters.quality Wool samples have for collected been analysis of farmers inthe Rajouri and districts herds. Reasi propagation native inthe track of Poonch, identified been for conservation and germplasm conservation strategy. Males of Poonchi have and other aid veterinary for improvement and with medicines (dewormer and anthelmithatic) Poonchi sheep population have provided been process. Computerizationsurveyed. of data is under from Jalore andfrom 119 flocks Barmer were Fivefrom sheep flocks Jodhpur 84flocks district, i.e.districts Jodhpur, Jalore and Barmer. Ninety was conductedMarwari sheep survey inthree sheep Marwari studied on transhumance Sheep spends tract. cent).(24.49 per Migratory route has been Haemonchus cent. 26.53per to be Among helminthes, the gastrointestinal parasitic infections was recorded 12.71 and bundle yield 76.56.Prevalence of staple diameter 29.08cmwith bundle strength wool and yield 700 gm staple length 11.86 cm and elasticityhigh and big pull with average annual and equal gloss, long fiber, compactness, high numbering 215 only. Wool is sub-white, of full Tibetan sheep population ascertained, has been Tibetan sheep spp. infection was predominant Marwari sheep Marwari medium Horns in size in males. are generally black incolour. infemales is small but Dewlap The eyelashesmuzzle, and hooves are mostly 47.3 cmwith forehead the slightly being concave. reared for Average purpose. milk facelength is arePurnea medium cattle tosized small mainly morphological and conformation characteristics. to age the of 3-7years have studied for been age of 0-3 years and 1074 animals belonging cattle, 1100animals inwhich belonging to the for studying characteristics the of Purnea Red for survey. far, So 948farmers were approached Araria, Pur CattlePurnea recorded far. so measurements of atotal of 86mithuns were also mithun owners with some inputs.Morphometric mithunthese rearing for villages motivating the and vaccination camps were conducted also at area Mithunsurvey population was 1670.Health work on survey the Nagaland Mithun. In the and Tuensang) were out for selected carrying inNagaland4 districts (Kohima, Phek, Longleng Mithun 14.83 percent during lean season. dynamics exists of with loosening weight upto months on alpine pastures. weight seasonal Clear approximately 6months and incold 6 desert Resear NBAGR nea andnea Katihar were districts selected ch Accomplishents Tibetan sheep to a smaller extentto asmaller and dung to agreater extent, and draught power providing substantial support interms of milk cattle plays role a vital in its breeding by tract Tail is long and switch is black incolour. Purnea round inshape. Teats are short and tube shaped. laterally and slightly forward. Udder and is small withsmall, an average 8.9cm,and being oriented cent respectively. Average density hectare of per areas isin surveyed 92.46,90.93and 95.78per have Proportion surveyed. been cattle of local ThreeSibasagar, district Sontipur Barpeta and Cattle of Assam grazing. main source of for drinking animals the during reported by farmers. the River water is the Nopurpose. major reproductive problems are are mainly for used ploughing and threshing around 2 to 5 kg of in a day. milk Bullocks and long face, mostly white incolour and yield medium sizewith prominent horns, narrow prefer Binjharpuri. local Binjharpuri are of byvillages farmers. the Resource farmers poor and Jersey crossbred cattle are reared also inthe total cattle population, reared. Graded Hariana Bhadrak. Binjharpuri forms around 50%of the adjoining Jajpur, districts Kendrapara and work outSurvey is carried being inthree Binjharpuri Cattle Purnea cattlePurnea jk-i-vk-la-C;wjks

39 ANNUAL REPORT 2014-15 network project on angr 40 ANNUAL REPORT 2014-15 network project on angr Kosali Cattle only.through natural service work power. Breeding of animal the is generally concentrate. They are rearedboth for and milk on natural with provision grasses of little bit of family is 1.85acre.cattle These are mostly fed and 5.11respectively. Average land holding per cultivable land for is 5.12, 4.26 three the districts backyard as well as scavenging system, of carcass is are 72-75%. They reared under Adult weight body is Dressing 47 Kg. percentage and weaning and is 250gm respectively. 5.5kg, to back along spine. the Average weight at birth Bristles are thick and long and spread from head black incolour with long snout and ears. small size with stout body. They are predominantly population of area. the pigs Doom are of medium pigs (Doom) arelocal major contributor of pig mainthe pig rearers of Dhemaji Mostly District. Assamese, Hajong and communities Bengali are inDhemaji.surveyed Missing, Nepali, Bodo, 846 pigs of 301villagers were identified and Pig population of Assam only availability seasonal of green the fodder. fodder source for animals is paddy straw with stuffs feed poor the availablethe in state. Main resistanceand disease and can thrive well under They have excellentcapacity of heat tolerance is commonly red followed by white and black. “BYASI”. called is locally paddythe crop or aeration crop in the and it pairsBullock are for used cleaning of from weed horn, horizontal ear production. and milk poor in sizewith stumpy, and small slightly inward are sub marginal/marginal. Animals are smaller have covered. been Approximately 70%farmers work. forselected survey Atotal of 1093animals (Rajnandgoan, bazaar and Bilaspur) Baloda were Three districts of plain region Chhattisgarhof jk-i-vk-la-C;wjks NBAGR Coat colorCoat of breed Resear ch Accomplishents stations launched. has been from Bikaner, Jodhpur, Udaipur and Kota Radio anCamel Aakashwani inparticular programme awareness about Resources Animal Genetic and recording initiated. field in the been has To create owners and 262camels recorded. has been Milk Mewari Camel and information from 43camel encompassing initiated has 11villages been for camels recorded. has been in6districts Survey information from 48camel owners and 126 initiated hasvillages been for Jalori Camel and encompassing 2districts inthe Survey 10 Mewari and Jalori camel on 700 animal. production Milk was recorded for Pradesh was conducted were and survey selected West Kameng and Tawang of District Arunachal yak Arunachali Arunachali yak Pig of Assam per familyper was recorded 0.47 to hectare be in the were for selected survey. Average land holding Sonitput, Three districts Sivsagar Barpeta and of AssamDuck and are ear lobes red. red, black and spotted (black and white). Comb pots. Mostin earthen prevalent colors are dark or houses. closed Water is provided to birds the nightDuring birds are kept cages bamboo inthe keeping commonof poultry is very areas. inthese meat and fighting). gameBackyard (Cock system ranges from 5to 30birds and are they kept for Deogard were covered under study. the size Flock Mayurbhanj,Three districts Keonjhar and Hazra chicken Sheep breeders maintain 1-3donkeys usually. to hock, the but in few donkeys it is beyond hock. In most of donkeys the tails is either above or up appearance. Colour of skinis black indonkeys. straight. The havefoals long hairglossywith are of medium length, dull inappearance and muzzle, eyes, legs and are belly common. Hair were recorded. Ears are erect. White markings of dark outline markings, light colored under parts on legsof few donkeys,observed shoulder strip, forehead is convex inshape. Zebramarking is is prominent. Nasal bone is concave, whereas in colour. Tail switch is non-distinct and pole donkeys are light grey, dark grey and brown assessing phenotypic their characteristics. The age) from Bikaner were district recorded for (including foals of seven less than one year of Twenty two biometric indices on 50 donkeys of RajasthanDonkeys was organized during 08-10 Resources (Characterization of Yak) Arunachali programme on Documentation of Yak Genetic three location and data was analyzed. Atraining Resear NBAGR ch Accomplishents th September, 2014. aspects ataspects LRSlamfarm, Guntur. feeding, breeding and various management conducted on importance of Ongole cattle, was found 45%.Awareness to be programs was donehas been in530cows and conception rate in Guntursemen Pregnancy district. verification by Goplamitrs and bulls LRSusing pedigreed inseminationsartificial have carried outbeen breedingthe In tract. atotal 40villages of 1050 identified for implementation project the in Guntur, Prakasam and Krishna were districts Ongole Cattle bulls have procured. been have procured. been Afterscreening, 5 young a research stationequipments in Bargur tract.The University has identified area and is establishing Bargur Cattle In situ Conservation of water for birds. these kitchen waste and bran. rice Pond is source the allowed for scavenging with supplementation of house during night the only. time Birds are area. Ducks aresurvey kept inconfined kutcha mainly Haringhata Black Chicken keepers under Cluster of approximately households fifty of Haringhata Black chicken Haringhata black chicken black Haringhata jk-i-vk-la-C;wjks

41 ANNUAL REPORT 2014-15 network project on angr 42 ANNUAL REPORT 2014-15 Network Project on AnGR dNTP, 50 pM of each primer and 0.5units of 50–100 ng of genomic DNA, 200μMof each μl on i-cycler. mixture Reaction consisted of inareactionwas performed volume of 25 spread across genome. the PCRamplification aiming to analyze polymorphic highly markers literature related with sheep diversity studies sheep population. were These chosen from were for used diversity the analysis of Tibetan markers (25)recommended for ovinenes using microsatellite markers. Microsatellite samples were prepared for diversity analysis quantity of isolated DNA was estimated and Phenol-Chloroform protocol. and Quality DNA from was using extracted whole blood Tibetan sheep (20)were processed and genomic samplesBlood from collected (5-6 ml) the variabilityGenetic inTibetan sheep start incubationstart of eggs. and 1.2±0.1kgfor hen. Households are ready to recorded at 165days was 1.6±0.03kgfor Cock approximately at 165days of weight age. Body first supply. The birds havereached intoegg at householdper was given aftertwo months of were out. carried supply Second of 50kgfeed their subsequent vaccination and deworming programme.the Management of birds the and Director, ARD, Govt. of West launched Bengal and 50kg feed. Vice Chancellor and Additional plastic (3lit drinker capacity), 100feet nylon net dewormed, one plastic (8kgcapacity), feeder one of 8weeks age that have vaccinated been and 25 birds. household Each given has 15 birds been assistance to construct or extend night shelter for age annually.Each Household was provided of 300Haringhata Black chicks of 4weeks household to challenge the take of production block. Agreement executed has with each been under Birohi Gram Panchayat of Haringhata boundariesSonakhali, was identifiedvillage in back yard and mode isolated by geographical jk-i-vk-la-C;wjks NBAGR Resear ch Accomplishents are pro being andfeed fodderand condition). health Samples compositionmilk parity, (season, lactation stage, was done as to variables so minimizethe affecting cattle(HFxSahiwal) from GLF, Hisar. Sampling Friesian), Bos indicus and (Sahiwal) cross bred from animals of selected Bos Taurus (Holstein metabolites.milk samples Milk were collected Biotechnology Incubator, Mohali for analysis of Farm, Hisar for of selection cows and Punjab establishedhas been with Government Livestock for identification of different metabolites.Liaison preparation that analyzed can be and quantified approaches to standardize protocols for sample samplesMilk were processed following different vitamins were shortlisted. protein, carbohydrates and minerals as well as literature, various metabolites milk of lipid, indigenous cows. After exhaustive of review nutritional or technological edge of from milk Bos taurus and crosses their canwhich explain metabolite profile of from milk Bos indicus, with objectives the to variations identify inthe cows. Thusthe project was planned and initiated or technological edge of from milk indigenous biomolecule/sthe can which explain nutritional profiles is as an essential pre- requisite to identify and exotic cattle. Estimation of metabolite milk metabolites present incow of milk indigenous a lack of understanding of differencesthe in characterized incattle, however there is clearly Major components of and crossbred cows characterization of indigenous, exotic based metabolomics Milk estimation. Data is analyzed being for genetic the diversity were analyzed on ABI-3100 DNA sequencer. for microsatellite all The loci. PCR products outcarried using aTouchdown programme Taq DNA polymerase. The amplification was cessed forcessed metabolite quantification. the milk has the milk been Awards • PatentsandTechnologies • Publications • ResearchProjects • Research Projects &Publications NBAGR jk-i-vk-la-C;wjks

43 ANNUAL REPORT 2014-15 jk-i-vk-la-C;wjks on & F u n cti a l G e no mics ati Ch aracteri z G e n etic 8. 7. 6. 5. 4. 3. 2. 1. Research ProjectsCompleted Research Projects RESEAR

March 2012,extended up to 31 (w.e.f.Sodhi January, 2012) -July 2008 to up to 24.02.11), RSKataria and Monika biomarkers -M Mukesh, (BP Mishra CCPI- for identification of probable mammary involution in indigenous cattle and buffalo and proteome during lactation and Analysis of gland transcriptome mammary Monika (w.e.f. Sodhi 01.4.2013). Gir and Tharparkarcattle -MS Tantia and Development of breed signature for Sahiwal, (NDRI, Karnal). biodiversity and DMallakar Aggarwal -RAK for cryopreservation of caprine genetic Utilization of cauda epididymal spermatozoa Ganguly and Sanjeev Singh. mammalian culture cell system -Indrajit plasminogen activator gene construct in Development and validation of human tissue (LUVAS, w.e.f. June, 2014). Karan Veer Singh, SJayakumar and ZSMalik in non-traditional animals (NTDA) dairy - of SNPs and association their traits with milk Profiling constituents,of milk identification and Karan Veer Singh. domesticthe populations yak -S Jayakumar Assessment of cattle genetic introgression in and VikasVohra (w.e.f. 14.07.2011). NeelPS Dangi, Kant RSBarwal, (GBPUAT) Uttar Pradesh and Uttarakhand -SP Dixit, genetic resources of Rohilkhand region of Characterization of non-descript goat Mishra, (CEO, OLRDS, Bhubaneswar). Arora,Reena Indrajit Ganguly and Sanat Koraput sheep -Sanjeev Singh, KNRaja, Phenotypic and genetic characterization of (NAIP). NBAGR CH PROJECTS &PUBLIATIONS st May, 2014. 9. 4. 3. 2. 1. On-Going Research Projects 11. 10.

2009 to March, 2012,Extended up to 30 for abiotie stress tolerance -RKVijh -April, Bio-prospecting of genes and mining allele SK Niranjan, Vikas Vohra (NBAGR), Sanat populationsbuffalo Odishaof -RS Kataria, Phenotypic and genetic characterization of 2014 to March, 2017. and Mohan Singh (CoVAS-CKV) -April, Chhatisgarh state -Vikas Vohra, RSKataria evaluation of population(s) buffalo of Identification, characterization and March, 2017. and Vijay - April, Bharti (DRDO) 2014 to cattle -Monika M Mukesh, Sodhi, RK Pundir Characterization and evaluation of ladakhi March, 2017. PS Dangi (from Nov, 2014) -April, 2014 to and states Sikkim - RK Pundir, PK Singh and indigenous cattle of meghalaya, Nagaland C 2009 to March, 2013, extended up to 30 MS Tantia up to 30.06.2011)-September, (w.e.f.10.07.2012) (BK Joshi up to 30.09.2013, KN and Raja VikasVohra Aggarwal RAK 01.10.13), Anand Jain, PK Vij, NKVerma, ecosystem -Arjava Sharmafragile (w.e.f. genetic resources for in livelihood security integration of plant animal and fish and agricultural intensification through Harmonizing biodiversity conservation 30 March, 2013, up to March, 2014 and up to April, 2010to March, 2012,Extended up to (up to 18.05.2012),BPrakash and PK Vij. Avnish Kumar, DK Yadav, Dinesh Kumar Bioinformatics Grid (NABG) in ICAR - Establishment of National Agricultural June, 2014(NAIP). June, 2014(NAIP). haracterization and evaluation of th June, 2014(NAIP). jk-i-vk-la-C;wjks th th

45 ANNUAL REPORT 2014-15 RESEARCH PROJECTS 46 ANNUAL REPORT 2014-15 RESEARCH PROJECTS 12. 11. 10. 9. 8. 7. 6. 5.

Deciphering molecular the basis of male jk-i-vk-la-C;wjks 2014) -April, 2014to March, 2018. (NDRI)De and Arora Reena (from Nov. Ahlawat, Sharma Rekha and Sachinandan sub-fertility or sterility inbovines -Sonika 2016. Imphal-Manipur) -April, 2014to March, MS TantiaRanadhir and Singh (CAU, Th. chicken (Kaunayen) of Manipur - PK Vij, Phenotypic characterization of indigenous 2015. Gantoor, AP) -April, 2014 to September, 31.12.14) and LRS, (Lam, MVDharma Rao SK Niranjan, RKVijh, (DK Sadana up to donkeys of Andhra Pradesh Behl, - Rahul Phenotypic and genetic characterization of 2015. (TANUVAS) - April, 2013 to September, Mishra, Indrajit Ganguly and PDevendran of Tamil Nadu PK -KNRaja, Singh, AK Characterization of indigenous dogbreeds PS Dangi - April, 2013to September, 2015. Verma, Sharma Rekha and Aggarwal, RAK Characterization of goats Sikkim -NK April, 2014to March, 2017. AroraReena and Dinesh Kumar Yadav- of Karnataka state- Anand Jain, VS Kulkarni, evaluation of known lesser sheep populations Identification, characterization and Anand Jain -April, 2012to March, 2016.) - Dinesh Kumar Yadav, Arora Reena and Classification ecotypes of Deccani sheep of to September, 2015. Sanjeev Singh (w.e.f.01.04.2014). April, 2013 Yashwant Singh (GADVASU-Bathinda) and -AKMishra, Vikastract KNRaja, Vohra and Characterization of Kajali sheep inits native 2014 to March, 2018. Mishra and SK (OUAT, Das Odisha) -April, RESEAR NBAGR CH PROJECTS &PUBLIATIONS 19. 18. 17. 16. 15. 14. 13.

2017. (DBT) Avtar Singh (NDRI) -8 Iquebal (IARI), Dinesh Kumar (IASRI) and Dixit, SJayakumar, AKDang (NDRI), MA and indigenous dual-purpose cattle -SP development of breed signatures for dairy Whole genome SNP mining based and 14)-April,Dec., 2014to March, 2017. AhlawatSonika and Ajit (PBTI-from Dua Sharma,Rekha MSTantia, Aggarwal, RAK of indigenous, exotic and crossbred cows - metabolomics characterizationMilk based Contd. MS Tantia (I/c,Network Project) -1992- Director NBAGR, Project coordinator and Resources - Arjava Sharma (w.e.f.01.10.2013) Network Project on Animal Genetic March, 2016. Sharma- up to 05.08.2014)-April, 2014to India -Avnish Kumar and PK Vij (Arjava system on farm animal genetic resources of Development of geographical information 2014 to March, 2017. Indrajit Ganguly and Sanjeev Singh -April, on Y-Chromosomebased markers specific - Haplotypic diversity of Indian cattle breeds 2018. Ganguly (LUVAS) -April, 2014to March, Venkataramanan (TANVASU) and Anita Ganguly, HKNarula KNRaja, (CSWRI)R Indian sheep breeds - Sanjeev Singh, Indrajit evaluation of heat stress protein genes in Molecular and physio-biochemical April, 2013to March, 2016. YAK), Taba Heli (KVK Pampumpare) - Kataria, Jyotsana TKBiswas Behl, (NRC- frontalis mithun(Bos grunnieons indigenous (Bos yak class II DR genes and in domesticated DQ Identification diversityallelic of of MHC ) -SK Niranjan, RS th July, 2014to 7 ) and and ) th July, July, 6. 5. 4. 3. 2. 1. Research papers Publications 20. RESEAR

A and Chakravarty AK(2015). Genetic D,Chakraborty Singh A,Tantia MS,Verma Biotechnology in genes:(CCL2) SNPs distribution analysis and monocyte chemo attaractant protein-1 bovinein the Toll like receptor 4(TLR4) Joshi polymorphisms BK (2014).Genetic Kimothi SP, R and M, Behl Bhatia AK, Sodhi JD,Behl Sharma A,Kataria RS,Verma NK, amb.2014.04.0001). Molecular Breeding mutton qualityinIndiansheep,Animal polymorphisms incandidategenesfor nucleotideIdentification ofnovelsingle Arora R, Yadav HSand Yadav DK(2014). Research, Ruminant genetics research of goat fecundity. status of molecular MS (2015). Current Ahlawat S,Sharm Journal of Dairy Science, 68:39-44. reproductive traits in Indian goats. analysis of anovel SNP inGPR54 gene with Tantia MS and Prakash V (2015).Association Ahlawat S, Sharma Maitra R, A, Borana K, 439–449. mutations incaprine Fec genes. primer ARMSPCRprotocol for genotyping optimization and validation of tetra- Designing,M andTantia MS(2014). Ahlawat S,Sharma Maitra R, A,Roy March, 2016.(National Fellow) -Manishibuffaloes Mukesh -May, 2011 to to diverse environment innative cattle and basis of tolerance thermo and adaptation Genome data mining to unravel molecular Bos indicus Bos NBAGR CH PROJECTS &PUBLIATIONS , 25(4):250–265. Animal cattle Sahiwal breed. Animal a R, Maitraa R, Aand Tantia 125: 34-42. , : 1-5 (doi: 10.5376/ (doi: 1-5 4: Meta Gene Indian Indian Small Small

2 : 11. 10. 9. 8. 7. 12.

Sciences, 85:161-164. of river buffalo. mammary gland derived osteopontin gene characterization and expressionanalysisof Mishra BP andKatariaRS(2015).Sequence Dubey PK, Goyal S, Mishra SK,Mukesh M, org/ 10.1016/j.livsci. 2014.06.007) Livestock Science 167:65-72.(http://dx.doi. promoter and inbuffalo other bovines. diversity analysis of thyroglobulin the gene MukeshR, Mand Kataria RS(2014).Genetic Kumari N,Mishra SK, Niranjan SK, Arora PK, Goyal S,YadavDubey BR, AK,Sahoo human Meta health. Gene, 4:45–56. affectingcaprine fatty milk acids related to of novel SNPs candidate in the genes Sharma Aand Nagda RK(2015).Association Dixit SP, Jayakumar S,Tyagi AK,Saroha V, journal.pone.0111609. buffalo. ruminantsdes petits ingoats virus and water (2014). Toll-Like receptor responses to Peste A, Kataria RS,Parida Sand Subbiah E. Ramya Raj GD, R, Tirumurugaan KG, Raja Dhanasekaran S,Biswas M,Vignesh AR, 40. minimization inbreed prediction using AandS, Rai Kumar D(2014).Locus Verma Jayakumar RAK, NK, Aggarwal Iquebal MA, Ansari Dixit MS,Sarika, SP, livsci.2014.01.028. http://dx.doi.org/10.1016/j. Science. horses. English Thoroughbred analysis amongsixIndianbreedsand RK (2014).Comparative genetic diversity N, Gupta SC, Pal Y, Tandon SNand Vijh Gupta AK ,ChauhanM,Bhardwaj A, Gupta cattle.Sahiwal Animal Science Reporter polymorphism of BoLA-DRB3.2 in locus PLoS ONE 9:e 111609. doi:10.1371/ Indian Journal of Animal of Journal Indian jk-i-vk-la-C;wjks Livestock , 9 :33-

47 ANNUAL REPORT 2014-15 PUBLICATIONS 48 ANNUAL REPORT 2014-15 PUBLICATIONS 15. 14. 13. 17. 16.

jk-i-vk-la-C;wjks Genetics networkneural artificial approach. Animal embryos in parthenote following in vivo transfer of and BhanjaSK(2014).Developmentof S, Vijh, RK,MalakarD,BagS,SarkhelB R, Rout PK, Agarwal SK, Goel, P, Saraswat Goel Kharche SD, Ranjan Jindal SK, AK, 10.1080/10495398.2014.955613. Animal Biotechnology (Bubalus bubalis) ofIndo-Gangetic Basin. Structuring ofRiverineBuffalo Population Assessment of Genetic Variability and U, Tantia MSand Vijh RK(2015). Joshi J,SalarRK,BanerjeeP, Sharma 888X.1000117. http://dx.doi.org/10.4172/2329- doi: 117. Cows. Stages ofLactation inSahiwal (Bosindicus) Mammary Epithelial Cells duringDifferent Carrier (SLC2A)GenesinMilkDerived M (2014).Expression Analysis ofSolute Mann S,Kataria RS,KaushikJandMukesh UK, Kishore A, Mohanty A, MishraBP, Jatav P, SodhiM,Sharma A, Shandilya dx.doi.org/10.4172/2329-888X.1000125 of India. Advances in Dairy Res ., 2:3;http:// inNativeGene indicus Cattle (Bos and Promoter Regions of β- Lactoglobulin Single Nucleotide Polymorphism inExon 4 Khate, Mishra M(2015). BP and Sodhi Kishore A,Mukesh RC, M,Sobti Keviletsu bubalis (Bubalus Indian native indicus cattle (Bos regionscoding of kappa-casein gene of of genetic variations across regulatory and RS, Mishra BP, M (2014). Analysis Sodhi Kishore A,Mukesh RC, M, Sobti Kataria 898. & DevelopmentalBiology- Animal 50: 893- Advances inDairyResearch,2 doi10.111/age.12208. Capra hircus. RESEAR ). Meta Gene NBAGR , 26:148-155, DOI: In Vitro Cellular CH PROJECTS &PUBLIATIONS , 2:769–781. ) and buffalo ) Breeds : 21. 20. 19. 18. 24. 23. 22.

in caprine MS (2014).Novel genetic polymorphisms Maitra A,Sharma Ahlawat R, Sand Tantia Sciences, 84:761-766. riverine buffalo. Indian Journal Animal of characterization of lactoferrin gene of Indian Kataria RS(2014).Cloning and sequence S, Niranjan SK, Gupta N,Prasad Aand Kumari PK, Singh N, Goyal S, Dubey Biotechnology and quality poor African semen. Journal of prognostic marker for differentiating good crossbred spermatozoa bull may not a be Sharma A(2014).Protamine 3expression in Mann S,Sengar G, Mandal DK, Kumar Mand Kumar S,Singh U, Ganguly Singh R, R, I,Deb 93: e108–e112. among crossbred bulls. Journal of Genetics, gene maysemen affect parametersquality at exonic region of aquaporin-7 (AQP7) KumarR, M and Sharma A (2014). SNPs D. Sharma K,Singh R, S,Sengar G,Singh Kumar Singh U, R, S,Deb Ganguly I,Mandal population using microsatellite markers. estimation goat type inBlack Bengal PS, and Verma diversity NK(2014).Genetic Mishra P. Dixit SP, Dangi RAK, Aggarwal of Animal Science 85:266-270. evaluation of Kalahandi goats. Journal Indian and microsatellite markers genetic based NK (2014)Phenotypic characterization Dash SK, Dangi PS, Tyagi Nand Verma Mishra P, Dixit RAK, SP, AS, Ali Aggarwal Reproduction Science, KiSS1 gene with reproductive traits. analysis of polymorphisms in caprine Roy Mand Prakash V(2014). Association Maitra A,Sharma Ahlawat R, S, Animal Sciences, 84: 1196–1201. reproductive functions. GPR54 , 13:1999-2003. gene associated with 151: 71–77. Indian Journal of of Journal Indian Tantia MS, Animal Animal 25. 31. 30. RESEAR 2 28. 27. 26. 9.

Journal of Biomedical and Life Sciences, microsatellite markers. DHR International Characterisation of Malvi Camel using Sharma Uand Vijh RK(2014).Genetic P,Prasad SA,Banerjee S,Ali Joshi J, 1050. Quantitative analysis of fatty acidinIndian Tyagi AK,Nagda RK and Dixit SP (2014). Saroha V, Kumar D, Sharma A,Jayakumar S, 268 Chemico-Biological Interactions, 220: 255- simulation calculation. and energy free peptidoglycanstype by molecular dynamics with meso-Diaminopimelic acidand Lysine- ofmodes buffalo PGRP1 receptor complexed Kataria RS(2014).Exploration of binding the SK,Lenka Maharana J, Pradhan SK and PK, Goyal S,Bhoi Dubey GK, BR, Sahoo Goats. issue, Special DHR-IJBLS Lineage and relationship of Himalyan Region Sharma Uand Vijh RK(2014).Evolutionary S,SinghRana S,Dureja V, Joshi J, P, Banerjee Wayamba Journal of Animal Science, Animal Research, 48:322-328. of cattle hill of Garhwal. Indian Journal of B (2014). Characterization and evaluation Kumar S,Tiwari Singh CVand R, Prakash Pundir RK,Singh PK, Neelkant, Sharma D, 84:974-977. evaluation. Tripura-characterisation and performance and Sadana DK (2014).Indigenous cattle of Pundir RK,Malik, S,Singh PK, Sharma D 7:65-70. Journal of Agriculture and Veterinary Science, bactrianus and Camelus dromedarius). IOSR SNPs and validation their incamel (Camelus U and Vijh RK(2014).Identification of P,Prasad SA,Banerjee S,Ali Joshi J, Sharma 286-296. NBAGR Indian Journal of Animal Sciences, CH PROJECTS &PUBLIATIONS , 5 (1):336-351. 7 :1044- 5 :

38. 37. 36. 35. 34. 33. 32.

and Technology, 5:110. Sikkim state.JournalofVeterinary Science Black: A newlyexploredgermplasmof Dangi PS and Bhutia NT (2014). Sikkim Verma NK, Aggarwal RAK,SharmaRekha, 85:397-403 bubalis). Indian Journal of Animal Sciences, in tissues of (Bubalus riverine buffaloes Differential expression of heat shock proteins UK, Kumari Pand Mukesh M(2015). M,KishoreSodhi A,Sharma A,Shandilya analysis. inKoraputbiometry sheep by regression (2014). Prediction Weight of Body from body Singh KN,Ganguly S,Raja Iand Arora R 25:69-72. Biotechnology, various livestock of species India. Animal exonthe 5of growth hormone gene inthe SC (2014). Comparative sequence analysis in Jayakumar S,Dixit SP, Gupta Nand Gupta Singh LV, Sharma A,Singh N,Kaur N, Sciences 85: 55–59. of Indian goat. Identification of novel SNPs in Mandakmale Sand Tantia MS(2015). Sharma Maitra R, A, Ahlawat S, Roy M, 42: 361-365. breeds. IGF1 and IGFBP-3 genes in nine Indian goat and genetic variability analysis of GHR, Dixit SP characterization (2014).Sequence Sharma A, Dutt G, Jayakumar S, Saroha V and of Biomedical and Life Sciences 5:352-361. of Indian Goat .DHR International Journal Sources of Variation of Fatty Acids inMilk Tyagi AK,Nagda RKand Dixit SP (2014). SarohaV, Kumar D, Sharma A,Jayakumar S, livestock. goat and milk its comparison with other Journal of Applied Animal Research, Indian Veterinary Journal Journal of Livestock Science, Indian Journal of Animal of Journal Indian INHBB jk-i-vk-la-C;wjks , 91: 24-27. 5: 1-8. gene

49 ANNUAL REPORT 2014-15 PUBLICATIONS 50 ANNUAL REPORT 2014-15 PUBLICATIONS 43. 42. 41. 39. 1. Technical Articles /popular 40.

jk-i-vk-la-C;wjks 58. goat. motif protein 3(RBM3)gene from Pashmina Molecular characterisation of RNA binding SM,Ahmed Vijh RKand Ganai NA (2015). Bhat MH,Naykoo NA,Khan F, Khan HM, Zargar, Urwat R, U, Malik F, Shah RA, 25:151-9. during Biotechnology lactation. Animal epithelial cells (MEC)inriverine buffalo (BAX enriched mammary inmilk and BCL2) transporter 1(GLUT1) and apoptotic genes (2014). Expression profiling of glucose RS, Yadav A,Mohanty AKand Mishra BP Yadav P, Singh DD, Mukesh M,Kataria in Veterinary Sciences, 99:129-36. andwith milk fat yield inbuffalo. Research (MEC) across lactation and correlation their epithelial mammary purified milk cells Kinetics of lipogenic genes expression in Yadav A, Mohanty AK and Mishra BP (2015). Yadav P, Kumar P, Mukesh M,Kataria RS, diversity among goats of Odisha. Characterization, Performance, and genetic Dixit SP, Dangi PSand SK Dash (2015). Verma NK,Mishra P, RAK, Aggarwal Characterization of Indigenous Animal Bioinformatics Approaches for Genome Short on Course Advanced Molecular and of farm animals, incompendium of ICAR- defining diversity populationand structure Arora R(2014).Microsatellite markers in Animal Sciences morphological traits. Indian journal of India based on microsatellite markers and sheep ofnorthwesternsemiaridzone discrimination ofMuzaffarnagri and Munjal Yadav DKand Arora R(2014).Genetic Journal of Animal Science, 85:165-171. Research in Veterinary Science, 98:51- RESEAR , 84:527–532. NBAGR CH PROJECTS &PUBLIATIONS Indian Indian , 6. 5. 4. 3. 2.

ICAR-Short course on Advanced Molecular to incompendium Bovine of lectures for Y-Chromosome Reference with Special Diversity(2014). Genetic of Mammalian N M, SinghGanguly S and Raja K I, Sodhi pp: 206-218. Resources,Genetic ICAR-NBAGR, Karnal. Characterization of Indigenous Animal Bioinformatics Approaches for Genome Short course on Advanced Molecular and in compendium of lectures for ICAR- genes Quantitative time through Real PCR M (2014). Expression analysis of candidate M andGanguly Mukesh I,Singh S,Sodhi Meerut. pp: 191-203. livestock genome analysis, ICAR-CIRC, tools and bioinformatics approaches for variations intraining manual on analysis and of an copy overview number gene expression PCRbased time Real Ganguly I,Ganguly Aand Singh S(2014). pp: 253-256 Resources,Genetic ICAR-NBAGR, Karnal, Characterization of Indigenous Animal Bioinformatics Approaches for Genome Short course on Advanced Molecular and in: compendium of lectures for ICAR- expression analysis using CLC bio software. handling of RNA data for seq differential analysis,short read preprocessing QC and Deshpandey Uand Singh S(2014).NGS pp: 52-60. Resources.Genetic ICAR-NBAGR, Karnal. Characterization of Indigenous Animal Bioinformatics Approaches for Genome onCourse Advanced Molecular and markers, incompendium of ICAR- Short approachesAnalytical for microsatellite Mand MukeshArora Sodhi R, M(2014). pp. 45-51. Resources.Genetic ICAR-NBAGR, Karnal. Molecular RESEAR 12. 11. 10. 9. 8. 7.

holder farmers inXIIAgricultural Science cattle breeds for of small livelihood security AK (2015).Crossbreeding versus Indigenous Sadana DK, Singh PK, Pundir RKand Mishra ICAR-NDRI, Karnal, pp: 35-47. management for livestock development areasin rural incompendium of Extension backyard for livelihood promotion poultry KNandRaja Mishra AK(2014).Prospects of Karnal, pp: 61-64. forward for organic farming, ICAR- NDRI farming practices: Away dairy on Good in compendium of Model training course animals for promoting organic farming conservation practices of indigenous dairy KNRaja and Mishra AK (2014). Good Karnal. pp: 7-14. analysis of phenomic and genomic data, training programme on advanced tools for compendium of lectures for National Data Analysis using Bioinformatics tools. KNandRaja Ganguly I(2015).Genome 97-102. phenomic and genomic data, Karnal. pp: program on advanced tools for analysis of compendium of lectures of National training values from phenotype and genotype in Pundir RK(2015).Predicting breeding pp: 169-174. Resources,Genetic ICAR-NBAGR, Karnal. Characterization of Indigenous Animal Bioinformatics Approaches for Genome Short course on Advanced Molecular and cattle. In: Compendium of lectures for ICAR- values from phenotype to genotype indairy Pundir RK(2014).Estimation of breeding pp: 76-85. Resources,Genetic ICAR-NBAGR, Karnal. Characterization of Indigenous Animal and Bioinformatics Approaches for Genome NBAGR CH PROJECTS &PUBLIATIONS 17- 16. 15. 14. 13. 18- 19-

NDRI Karnal from 3 Congress (compendium), held at ICAR- Animal Pro Animal Himalayanthe In: foothills, Joshi Catle pastoralism in BK.2014.Belahi: Vohra V, Niranjan SK,Mishra AKand Karnal, pp: 242-247. Resources,Animal Genetic ICAR-NBAGR, for Genome Characterization of Indigenous Molecular and Bioinformatics Approaches lectures for ICAR-Short course on Advanced generation and analysis incompendium of Generation platform for RNA the data seq Singh S and Ganguly I(2014).Next Research and diagnosis. International Journal Science of microarray and its applications indiseases Singh Sand MK(2014).DNA Bedekar pp: 219-223. Resources,Genetic ICAR-NBAGR, Karnal, Characterization of Indigenous Animal and Bioinformatics Approaches for Genome ICAR-Short course on Advanced Molecular incompendiumReaction of lectures for time-QuantitativeReal Polymerase Chain and Mukesh M(2014).RNA Isolation and Sharma A,Ganguly I, Thakur K, P Verma pp: 10. ifjn`';A i'kq/kuizdk'k5 dk (2014)AIyku vkbZ 'kqdj xkaxqyh vkSj vkj mik;A i'kq/kuizdk'k5 Tokj vkSj dSfYl;e dh deh dks fu;fU=r djus ds (2014)AnqX/k vkbZ esa xkaxqyh i'kq/ku vkSj Ms;jh ,l flag oh] HkuksV ,l] vkj fclyk ,] xkaxqyh o.Z ,a gowk xsak lw% kh rFkk ds ukjh lapkSjhA i'kqèkuizdk'k]5 lewg% jkT; xksoa'kh egRoiw.kZ jktLFkku ,oa vof.kZr (2014)- ds vkSj ds Mh vlhtk lnkuk ds] vkj iqaMhj ds] ih flag ckfud ,l] iadt ih ds] uLdj ,l] iq:"kksÙke ,l] uLdj ds] ih iadt ,l] ckfud d.,50: 317. , 3:1572-1573. % 106&108- rd % 1&7- %33&35- Feb to 6 Proc. Aust Soc th jk-i-vk-la-C;wjks Feb 2015,

51 ANNUAL REPORT 2014-15 PUBLICATIONS 52 ANNUAL REPORT 2014-15 PUBLICATIONS 2. 1. 22- oekZ ,u ds,u oekZ (2014)A22- cdfj;ksa dh çns'k flfDde (2014)AvkbZ xkaxqyh vkSj ,u ds jktk ,l] flag 21 (2014)A,l flag vkSj ,l xks;y ih] ,l flag 20- 25- 24- eksfudk eqds'k] euh"kh xksj[key] flag 23-chjcy 26- 27- Books Published Books

jk-i-vk-la-C;wjks of Quantitative Trait for yield, Loci fat milk Vijh RKand S(2014)Identification Gokhale Research inBuffaloes. projects area inthe of and Basic Strategic out in NAIP and future of perspective the and presented history, the work carried byBank NAIP and consisted of sixchapters wasbook (2014). Thesubmitted the to World - ASynthesisFocal species by RKVijh NAIP Project Componentthe 4Buffalo as dk xq.kfu/kkZj.kAi'kq/kuizdk'k5 5 izdk'k] i'kq/ku uLyA HksM+ Kkr dke dksjkiqV&,d izdk'k 5 i'kq/ku mi;ksfxrk dh ikyu cdjh eas çns'k mÙkj vkSj egRo]i'kq/kuçdk'k]5 ewY; dk lalk/kuks vuqokaf'kd i'kq esa ifjfLFkfr;ksa euh"kh dfBu dh {ks= ,oa yík[k & (2014)ysg eqds'k dqekjh ços'k çHkkr Hkkjrh] eku] fot; lanhi dqekj oekZ] çhrh lks<+h] eksfudk 5 çdk'k] /ku i'kq js[kk% thou dh tkfr ekuo & lanhi eku ,oa eksfudk lks<+h (2014) tSo fofo/krk euh"kh eqds'k] ços'k dqekjh] ch ds tks'kh] çhrh oekZ] tSofofo/krk lja{k.k] i'kqq/ku çdk'k] lks<+h] ,oa lqjs'k flaxyk (2014) Dyksfuax }kjk i'kq csgrj fodYi]i'kq/kuçdk'k dk nw/k ds xk; nw/k dk (2014) cdjh VkafV;k ,e,l vgykor lksfudk 'kekZ js[kk nsoh] lfork (2014)- jktLFkku jkT; ds vof.kZr ,oae ds- egRoiw.kZ vfltk o Mhds lnkuk jkds] iqanhj ihds] flag xksoa'k lewg%ukjhrFkklapkSjh]i'kq/kuçdk'k]5 % 8&10- % 60&66 % % 36&42- RESEAR NBAGR % 49&59- % 5 % 67&72 CH PROJECTS &PUBLIATIONS % 19&22- 5 % 26&29- % %1

3. 3. 2. 1. ChaptersBook

publishing house. ISBN 978-93-84053-01-7 for livestock genome analysis. Satish Serial Ganguly I(2015).Bioinformatic approaches Dhama R, Alex Kand RR, Alyethodi R, Singh U, Kumar S,Mukhopadhyay CS,Deb Component 4on 19-20 ICARGeneral, at Terminal the Workshop of ICRISAT,DG FAO representative, Director World and Bank was released by World Bank, was meeting Final with released inthe the published by Component 4NAIP. book The Reference Family -Germplasm Catalogue and protein percentage in buffaloes- Buffalo "Biotechnology: Progress and Pro in Farm Animals” for compiled book Applications of Trangenesis and Cloning “MethodologiesAnimals insection and of Transgenesis and Cloning inFarm M(2015). Methodologies and Applications Joshi M, Sharma R and BK, Sodhi Mukesh Chapter 7,pp: 157-178. publishing house. ISBN 978-93-84053-01-7. for livestock genome analysis. Satish Serial m-RNA levels inBioinformatic approaches PCR time for(2014). Real Quantification of Ganguly I, Ganguly A,Singh S and Singha H Circular Motijheel, Road, Bee, Dhaka- 1000. Tithi Printing and Packaging, 28/C-1Toyen 249. ISBN No. 978-984-33-8230-6.Printed at Farmgate, Dhaka-1215, Bangladesh. Pp: 119- Agricultural Centre, BARC Complex, and Management. Published by SAARC SAARC Countries: Diversity, Conservation In Farm Resources Animal Genetic in Diversity, and Conservation Management. ResourcesAnimal Genetic in India: Singh PK and Kumaresan A(2014).Farm Sriv astva AK,Pathak KML,Joshi BK, th March, 2014. spects Eds: 6. 5. 4. 3. 2. 1. Monograph/Bulletin/Compendium RESEAR 5. 4.

Bioinformatics Approaches for Genome short course on “Advanced Molecular and A Training compendium on ICAR sponsored and Bhutia NT. Sharma RAK, Dangi PSNK, Aggarwal R, explored germplasm (#86/2015)by Verma A leaflet entitled Black ‘Sikkim goat-A newly ISBN: 978-93-83537-19-8. NK and Mishra PMonograph #84/2014, germplasm of Bundelkhand region by Verma Bundelkhandi Goat- An important 93-83537-18-1. Ganguly I.Monograph #85/2015.ISBN-978- sheep by Singh S, Raja KN, Arora R and ResourcesSheep Genetic of India- Koraput Resources,Genetic Karnal. RK by ICAR-National Bureau of Animal (# 83)by Vij PK, Tantia MS,Pan Sand Vijh Chicken breeds of India –Harringhata Black Resources, Karnal. ICAR- National Bureau of Animal Genetic Mishra AK,Joshi BK and Sharma A,by byhills Vohra V, M,Niranjan Sodhi SK, migratory cattle from northHimalayan foot Cattle Resources Genetic of India “BELAHI: Jodhpur, Rajasthan, Chapter 2,pp. 22-36. Health Publisher, M.K.). Scientific (Ed. Rana, and human inVegetables health and Human Ganguly Aand Ganguly I(2015).Climate change Chapter 1,pp: 1-14. publishing house. ISBN 978-93-84053-01-7. livestock genome analysis. Satish Serial analysis in Bioinformatic approaches for bioinformatics tools for molecular data KNandRaja Ganguly I(2015).Basic Studium Press LLC, Texas, U. SM Paul Khurana and Machiavelli Singh , NBAGR CH PROJECTS &PUBLIATIONS 11. 10. 9. 8. 7.

2015. trainingthe held from toJanuary, 28th 30th to Application Vijh RK and Jeere A (2015) for Sequencing Data Analysis: From Technology Training Manual on Next Generation Leaflet No. 87/2015. Niranjan M(2015).NBAGR SK and Sodhi meat by &milk Kataria PK, RS,Dubey Test for differentiation cattleof and buffalo by NBAGR, Karnal. Verma Pand Mukesh M(2014). Published N, Kishore A,Kataria RS,Mann S,Kumari P, by Shandilya M, Kapila UK, Sharma A, Sodhi culture of buffalo epithelial mammary cells Establishment of three-dimensional (3D) NBAGR, Karnal, Haryana-132001. Dang Aand Grover SPublished by ICAR- N, Singh S,Kumar S,Malakar D, Kaushik JK, Khate K, Mishra BP, Mohanty A , Varshney Chandra A,Verma P, Kumari P, Yadav N, Kaur Tantia R, MS,Kataria RS,Ganguly I, N, Jatav P, Yadav P, J, Aggarwal Miglani M, Kishore A,Shandilya UK,Mann S,Kapila buffaloes by M, Sharma Sodhi A, Mukesh M, transcriptomic research in Indian cattle and Reference gene panels for application in Singh S,Malakar Dand Kaushik JK. Mann S,Verma P, Kumari P, Mohanty A, M,KapilaSharma A,Sodhi N,Kataria RS, culturecell by Mukesh M, Shandilya UK, analysis with conventional monolayer (2D) cells (BuMECs) and comparative expression culturecell of buffalo epithelial mammary Development of three dimensional (3D) December, 2014. RS held at NBAGR, Karnal from 01to 10 Ganguly I, Singh S,Thakur K and Kataria Resources”Genetic by Mukesh M, M,Sodhi C haracterization of Indigenous Animal jk-i-vk-la-C;wjks

53 ANNUAL REPORT 2014-15 PUBLICATIONS 54 ANNUAL REPORT 2014-15 PATENTS AND TECHNOLOGIES 6. 5. 4. 2. 1. applications are as follows as: Patent Office, detailsDelhi.New The these of applications were submitted to Indian the first examination requests for three patent Journalthe of Indian Patent and the Office pateSeven nt applications were published in Patents andTechnologies 3.

jk-i-vk-la-C;wjks et al et 2426/DEL/2013) invented by Dr. Vijh R.K. percent in buffaloes” (application number Technology entitled “QTLs for fat milk et al et 1890/DEL/2013) invented by Dr. Vijh R.K. count inbuffaloes” (application number Technology entitled “QTLs for somatic cell was published on 2 DEL/2013) invented by Dr. Vijh R.K. al et in buffaloes” (application number 1889/ Technology entitled “QTLs for yield milk on 22 invented by Dr. Vijh R.K. al et (application number 298/DEL/2013) verification in Indian RuminantLivestock” Technology entitled “A Kit for parentage was published on 22 50/DEL/2013) invented by Dr. Vijh R.K. al et verificationGoats” in (application number Technology entitled “A Kit for parentage was filed was filed on 5 FER was filed FER was filed on 14 was published on 12 DEL/2013) invented by Dr. Kataria al R.S. et meat and milk” (application number 607/ for differentiation the cattleof and buffalo Technology entitled “PCR DNA based test Jan 2015. was published on 20 was published on 2 nd August 2014and FERwas filed on 5 RESEAR th Jan 2015. nd NBAGR th nd January 2015. th November 2014. August 2014and FER September 2014 and nd th January February 2015. CH PROJECTS &PUBLIATIONS was published published was 2015. th

4. 3. 2. 1- Awards 7.

2015. Congress at NDRI Karnal from Feb 3-6, platform” during XII Agricultural Science gland using heterologous bovine microarray transcriptome signature of buffalo mammary poster award on “Establishing stage specific Ranjit SKataria and Manishi Mukesh. Kishore, Ashok KMohanty, Ajay Dang, Mishra, Monika Anita Yadav, Sodhi, Amit Sharma,Ankita BP Jigyasa Aggarwal, CSWRI, Avikanagar on 12 during National the Sheep and Wool Fair at Award of for Stall Exhibition the Best the at TANUVASU, Chennai, Feb.13-14, .2015. & XIIannual convention of SOCDAB, held indevelopinglivelihood security countries management of Animal genetic Resources for in international symposium on Sustainable unexplored canine genetic resources of Inda, Charecteriztion of Chippiparai dogbreed- an PosterKathirvel S.2015. Best award: 2015: I, K N, Raja al et 2427/DEL/2013) invented by Dr. Vijh R.K. percent in buffaloes” (application number Technology entitled “QTLs for protein milk C;wjks] djuky132001gfj;k.kkA foHkkx] jktHkk"kk flrEcj] 2014] jk"Vªh; i'kq vkuqoaf'kd lalkèku ys[k] vuqla/kku iqjLdkj] çFke }kjk foHksnhdj.k- ij vk/kkj cgq&#ihfof/k ds eki esa 'kkjhfjd xk;ksa ukjh o lkapksjh dkadjst] dh jktLFkku 2014- lMkuk- dqekj nsfoanj o flag dqekj çeksn iqaMhj] dqekj jkds'k Devemndran P, Saravanan Rand was publish Singh P K, Mishra A K, Ganguly ed oned 20 th th February 2015. November 2014. Best Best RFDevaluationreport2013-14 • RFD2014-15 • Result Framework Document NBAGR jk-i-vk-la-C;wjks

55 ANNUAL REPORT 2014-15 56 ANNUAL REPORT 2014-15 RFD 2014-15 6. 5. 4. 3. 2. 1. Functions 3. 2. 1. Objectives To protect and indigenous conserve animal genetic resources for sustainable utilization and livelihoodsecurity Mission and conservation. Striving for excellence ininnovative research to genetic identify potential of indigenous livestock for improvement Vision

jk-i-vk-la-C;wjks genetic resources. Creating awareness about maintenance, the conservation and management of farm animal level. Capacity building field inthe of molecular biotechnology for breed characterization at genome Establishment and maintenance of data on bank farm animal genetic resources of India. Characterization of important functionally genes of farm animal species. ofConservation economically important and endangered breeds of farm animal species. Characterization and evaluation of livestock breeds and of poultry country. the utilization of animal genetic resources. Human resource development area inthe of identification,evaluation, characterization and Documentation on institute activities. resources Identification,evaluation, characterization and conservation of indigenous farm livestock genetic National Bureau Resources, of Karnal Animal Genetic Result Framework (RFD) Document Address: P.B. No. byepass, 129,GTRoad Near Basant Vihar NBAGR Section 1:Vision,Section Mission, and Objectives Functions Website: www.nbagr.res.in (2014-2015) Karnal –132001 for Result framew Section 2: Inter se priorities among Key Objectives, Success Indicators and Targets

S. Objective (s) Weight Action (s) Success Unit Weight Target/Criteria value NBAGR No. Indicator (s) Excellent Very Good Fair Poor Good 100% 90% 80% 70% 60% 1 Identification, evaluation, 60 Phenotypic/ genetic Breed / Number 20 8 7 6 5 4 characterization characterization and population and conservation of evaluation of AnGR / strain indigenous farm livestock characterized genetic resources

Part / complete Gene fragment Number 20 38 32 26 20 14 ork document characterization / characterized expression profiling of gene Conservation of Semen doses Number 20 16200 13500 10800 8100 5400 germplasm cryopreserved / disseminated 2 Documentation on 12 Breed descriptors / Documents Number 12 13 11 9 7 5 institute activities monographs / bulletins / published manuals / leaflets 3 Human resource 8 Awareness programmes Awareness Number 8 11 9 7 5 3 development in the & capacity building on programmes area of Identification, AnGR through trainings / events evaluation, and exhibitions organized characterization and utilization of animal genetic resources

* Publication and 5 Publication of the research Research No. 3 20 18 16 14 12 jk-i-vk-la-C;wjks Documentation articles in the journals articles having the NAAS rating published of 6.0 and above

57 ANNUAL REPORT 2014-15 RFD 2014-15 58 ANNUAL REPORT 2014-15 RFD 2014-15

S. Objective (s) Weight Action (s) Success Unit Weight Target/Criteria value jk-i-vk-la-C;wjks No. Indicator (s) Excellent Very Good Fair Poor Good 100% 90% 80% 70% 60%

Timely publication of the Annual Report Date 2 30 June, 02 July, 04 July, 07 July, 09 July, Institute Annual Report published 2014 2014 2014 2014 2014

(2013-2014) NBAGR Result framew * Fiscal resource 2 Utilization of released Plan fund % 2 99 98 96 94 92 management plan fund utilized

* Efficient Functioning of 3 Timely submission of On-time Date 2 15 May, 16 May, 19 May, 20 May, 21 May, the RFD System Draft RFD for 2014-2015 submission 2014 2014 2014 2014 2014 for Approval

Timely submission of On-time Date 1 May, 1 May 2, May 5, May 6, May 7, Results for 2013-2014 submission 2014 2014 2014 2014 2014

* Enhanced Transparency 3 Rating from Degree of % 2 100 95 90 85 80 / Improved Service Independent Audit implementation

delivery of Ministry/ of implementation of ork document Department of Citizens’ / Clients’ commitments Charter (CCC) in CCC

Independent Audit Degree of % 1 100 95 90 85 80 of implementation success in of Grievance Redress implementing Management (GRM) GRM system

* Administrative Reforms 7 Update organizational Date Date 2 Nov.1 Nov.2 Nov.3 Nov.4 Nov.5 strategy to align with 2014 2014 2014 2014 2014 revised priorities Result framew S. Objective (s) Weight Action (s) Success Unit Weight Target/Criteria value No. Indicator (s)

Excellent Very Good Fair Poor NBAGR Good 100% 90% 80% 70% 60%

Implementation of % of % 1 100 90 80 70 60 agreed milestones of Implementation approved Mitigating Strategies for Reduction of potential risk of corruption (MSC). ork document Implementation of % of % 2 100 95 90 85 80 agreed milestones for implementation ISO 9001

Implementation of % of % 2 100 90 80 70 60 agreed milestones of implementation approved Innovation Action Plans (IAPs). jk-i-vk-la-C;wjks

59 ANNUAL REPORT 2014-15 RFD 2014-15 60 ANNUAL REPORT 2014-15 RFD 2014-15

Section 3: Trend Values of the Success Indicators jk-i-vk-la-C;wjks

S. Objective Action Success Indicator Unit Actual Actual Target Projected Projected No. Value Value Values for Values Value for for FY for FY FY 2014- for FY FY 2016- 2012- 2013- 2015 2015- 2017 2013 2014 2016

1 Identification, evaluation, Phenotypic/ genetic Breed/ population/ Number 5 7 7 8 9 NBAGR characterization and characterization and evaluation strain characterized Result framew conservation of indigenous of AnGR farm livestock genetic resources Part/complete characterization / Gene fragment Number 18 32 32 34 35 expression profiling of gene characterized Conservation of germplasm Semen doses Number 11500 15000 13500 14000 14500 cryopreserved / disseminated 2 Documentation on institute Breed descriptors / monographs / Documents Number 9 11 11 12 13 activities bulletins/ manuals/leaflets published 3 Human resource Awareness programmes & Awareness Number 7 9 9 10 11

development in the area of capacity building on AnGR programmes / ork document Identification, evaluation, through trainings and events organized characterization and exhibitions utilization of animal genetic resources * Publication and Publication of the research Research articles No. 35 18 18 20 22 Documentation articles in the journals having the published NAAS rating of 6.0 and above Timely publication of the Annual Report Date - - 30 June, - - Institute Annual Report (2013- published 2014 2014) * Fiscal resource Utilization of released plan fund Plan fund utilized % 98.7 98.8 96 98 99 management Result framew S. Objective Action Success Indicator Unit Actual Actual Target Projected Projected No. Value Value Values for Values Value for for FY for FY FY 2014- for FY FY 2016- NBAGR 2012- 2013- 2015 2015- 2017 2013 2014 2016 * Efficient Functioning of the Timely submission of Draft RFD On-time Date - - 16 May, 14 - - RFD System for 2014-2015 for Approval submission Timely submission of Results for On-time Date - - May 2, - - 2013-2014 submission 2014

* Enhanced Transparency / Rating from Independent Audit Degree of % - - 95 - - Improved Service delivery of implementation of Citizens’ / implementation of ork document of Ministry/Department Clients’ Charter (CCC) commitments in CCC Independent Audit of Degree of success % - - 95 - - implementation of Grievance in implementing Redress Management (GRM) GRM system * Administrative Reforms Update organizational strategy to Date Date - - Nov.2, - - align with revised priorities 2014 Implementation of agreed % of % - - 90 - - milestones of approved Implementation Mitigating Strategies for Reduction of potential risk of corruption (MSC). Implementation of agreed % of % - - 95 - - milestones for ISO 9001 implementation Implementation of milestones % of % - - 90 - - jk-i-vk-la-C;wjks of approved Innovation Action implementation Plans (IAPs).

61 ANNUAL REPORT 2014-15 RFD 2014-15 62 ANNUAL REPORT 2014-15 RFD 2014-15

Section 4 (a): Acronyms jk-i-vk-la-C;wjks

S.No Acronym Description 1 AnGR Animal Genetic Resources 2 A.H Animal Husbandry 3 KVK Krishi Vigyan Kendra 4 NGO Non Government Organization NBAGR

5 SAU State Agricultural University Result framew 6 ICAR Indian Council of Agricultural Research 7 IPR Intellectual Property Right 8 NBAGR National Bureau of Animal Genetic Resources 9 SVUs State Veterinary Universities 10 CCC Citizens’ / Clients’ Charter 11 IAPs. Innovation Action Plans 12 MSC Mitigating Strategies for Reduction of potential risk of corruption

Section 4 (b): Description and definition of success indicators and proposed measurement methodology ork document

Success Indicator Description Definition Measurement General Comments

1 Breed / Evaluation and characterization means Country has a large animal diversity Number The undefined animal population population data generation on morphological, which needs to be defined in across different species needs to / strain bimerical traits, on animals in their terms of phenotypic and genotypic be defined as breeds / populations characterized respective native tract. Genetic characterization so as to assign them with unique characters so as to characterization will involve the status of a breed or a population have a well defined breeding microsatellite based genotyping. with unique characteristics and plan for their improvement / Information on performance traits thereafter initiate programme for conservation. through interaction with farmers is their improvement and conservation also included. depending on their number. Result framew

Success Indicator Description Definition Measurement General Comments NBAGR 2 Genes fragment The gene fragment is characterized to The traits are controlled by whole/ part Number Gene characterization forms a characterized know the genetic diversity at allele/ gene. To understand the level and extent part of genetic diversity study. nucleotide/aminoacid level. of genetic variability exhibited by a trait it is necessary to know the expression profile of a gene fragment controlling a particular trait.

3 Semen doses The ex situ conservation will be in the Cryopreservation of semen is an ex situ Number These are the methodologies used cryopreserved/ form of cryopreservation of semen. method of conserving the breed for for conservation/improvement/ disseminated Semen will also be distributed for either posterity or/and their use for faster faster multiplication of breeds/ ork document utilization in the field. multiplication of germ plasm. strains outside their natural habitats depending on the need in view of their available numbers or uniqueness of character(s).

4 Documents Documents includes the breed After completing the characterization of a Number Documentation is important in published descriptor, breed monograph, leaflet, breed/population/strain, the information this era of IPR/patent to save the training manual etc. generated need to be compiled in the interest of stake holders. form of a document which may be in the form of descriptor / monograph/ leaflet. Training manual is prepared for the trainee participants. It provides the information on knowhow in the field of AnGR characterization and conservation.

5 Awareness Programmes / events refer to the The awareness programmes help in Number Awareness programmes are programmes / exhibitions / fares organized to create updating the knowledge of the stake necessary for sensitization events the awareness and capacity building holders, field functionaries and animal of the people involved in the among masses on AnGR. husbandry department officials dealing conservation and improvement jk-i-vk-la-C;wjks with the indigenous animal genetic programmes of animal genetic resources resources.

63 ANNUAL REPORT 2014-15 RFD 2014-15 64 ANNUAL REPORT 2014-15 RFD 2014-15

Section 5: Specific performance requirements from other departments that are critical for delivering agreed results jk-i-vk-la-C;wjks

Location Type State Organization Organization Relevant What is your Justification for this requirement Please What happens Type Name Success requirement from quantify your if your Indicator this organization requirement requirement is not met STATE All states of SAUs/State SAUs/State Breed/ To locate true to Since the AnGR is located in 20% The target India departments of departments of population/ type animals for different states of the country. value will be measurements and AH, KVKs and AH, KVKs strain Interaction is required with farmers affected. NBAGR NGOs, SVUs characterized blood sampling and to in their local language interact with farmers in Result framew their local language..

STATE All states of ICAR ICAR Semen doses Collaboration for NBAGR is not having any animal 20% The target India Institutions/ Institutions, cryopreserved / collection of semen farm of its own. Since the AnGR value will be SAUs/State AH SAUs/State disseminated doses. is located in different states and affected. departments of departments, Institutions of the country AH, KVKs and and NGOs NGOs,SVUs

Section 6: Outcome / Impact of activities of Department/Ministry

S.N. Outcome/Impact Jointly responsible for Success Indicator (s) Unit 2012-13 2013-14 2014-15 2015-16 2016-17 influencing this outcome/ impact with the following ork document Organizations/Departments/ Ministry (ies)

1 Enhancement in State AH departments, Livestock Breed / Population / strain Number 5 7 7 8 9 number of descript Development Board, State characterized breeds Veterinary Universities, NGOs 2 Cryopreserved State Livestock semen banks Semen doses preserved/ Number 11500 15000 13500 14000 14500 germplasm for disseminated posterity. 3 Documents - Documents published Number 9 11 11 12 13 developed on AnGR 4 Enhanced knowledge - Awareness programmes Number 7 9 9 10 11 and capacity building organized & capacity building on indigenous on AnGR through trainings AnGR and exhibitions Result framew Annual RFD Performance Evaluation Report for 2013-14

Name of the Division: Animal Science Division NBAGR Name of the Institution: National Bureau of Animal Genetic Resources, Karnal RFD Nodal Officer: Dr. NK Verma, S. Objective(s) Weight Action(s) Success Unit Weight Target / Criteria Value Performance Percent Reasons No. Indicator(s) Good Fair 70%Poor achieve- for short- ExcellentVery Raw Weight- 80% 60% ments falls or 100% Good Score ed Score 90% against Tar- excessive get values of achieve-

90% Col. ments, if ork document appli-

Achievements cable 1. Identification, 70 Phenotypic and Breed/ Num- 40 7 6 4 3 2 7 100 40 116.6 - evaluation, genetic charac- population/ ber character- terization and strain char- ization and evaluation of acterized conservation AnGR of indigenous Part/complete Genes frag- Num- 20 32 30 28 26 24 32 100 20 106.7 - farm live- characterization ment char- ber stock genetic and expression acterized resources profiling of gene Conservation of Semen doses Num- 10 12500 12000 10000 9000 8000 15000 100 10 125 Addition- germplasm conserved/ ber al efforts dissemi- and avail- nated ability of semen 10 12 10 8 7 6 11 95 9.5 110

2. Documenta- 10 Breed descriptors Documents Num- - jk-i-vk-la-C;wjks tion on insti- / monographs / published ber tute activities bulletins/ manu- als/leaflets

65 ANNUAL REPORT 2014-15 RFD EVALUATION REPORT 2013-14 66 ANNUAL REPORT 2014-15 RFD EVALUATION REPORT 2013-14

S. Objective(s) Weight Action(s) Success Unit Weight Target / Criteria Value Performance Percent Reasons jk-i-vk-la-C;wjks No. Indicator(s) Good Fair 70%Poor achieve- for short- ExcellentVery Raw Weight- 80% 60% ments falls or 100% Good Score ed Score 90% against Tar- excessive get values of achieve- 90% Col. ments, if appli-

Achievements cable NBAGR 3. Human 9 Ttrainings and Pro- Num- 9 9 8 7 6 5 9 100 9 112.5 - Result framew resource exhibitions re- grammes/ ber development lated with animal events orga- in the area of genetic resources. nized Identification, evaluation, character- ization and utilization of animal genet- ic resources 4. Efficient 3 Timely submis- On-time Date 2 15/05/1316/05/1317/05/1320/05/1321/05/1306/05/13100 2 100 - Functioning sion of Draft submission

of the RFD RFD (2013-14) ork document System for approval Timely submis- On-time Date 1 01/05/1302/05/1305/05/1306/05/1307/05/1317/04/13100 1 100 - sion of Results submission for RFD (2012- 13) 5. Administra- 4 Implement ISO % Imple- % 2 100 95 90 85 80 100 100 2 100 - tive reforms 9001 as per the mentation Improv- approved ac- On time Date 2 30/07/1310/08/1320/08/1330/08/1310/09/1324/07/13100 2 100 - ing internal tioWn plan submission efficiency / Prepare an action responsive- plan for Innova- ness / service tion delivery of Ministry / Department Result framew S. Objective(s) Weight Action(s) Success Unit Weight Target / Criteria Value Performance Percent Reasons No. Indicator(s) Good Fair 70%Poor achieve- for short- ExcellentVery Raw Weight- NBAGR 80% 60% ments falls or 100% Good Score ed Score 90% against Tar- excessive get values of achieve- 90% Col. ments, if appli-

Achievements cable 6. Improv- 4 Implementation Indepen- % 2 100 95 90 85 80 100 100 2 100 - ing internal of Sevottam dent Audit efficiency / of Imple- responsive- mentation ness / service of Citizen’s ork document delivery of Charter Ministry / Indepen- % 2 100 95 90 85 80 100 100 2 100 - Department dent Audit of public grievance redressal system

Total Composite Score: 99.50% Rating: Excellent jk-i-vk-la-C;wjks

67 ANNUAL REPORT 2014-15 RFD EVALUATION REPORT 2013-14 68 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics jk-i-vk-la-C;wjks NBAGR ExhibitionsOrganized • TrainingsAttended • TrainingsandWorkshopsOrganized • Training andCapacity Building NBAGR jk-i-vk-la-C;wjks

69 ANNUAL REPORT 2014-15 70 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics jk-i-vk-la-C;wjks NBAGR 3. 1. Trainings andW TRAINING ANDCAPACITY BUILDING 2. Organized

on 28.01.2015at NBAGR, Karnal. It was jointly by DAHD&F, GOI and NBAGR Registration Process’ was organized An interactive workshop on ‘Breed Persistent System, Pune during 28 at NBAGR Karnal incollaboration with Technology to Application" was organized Generation Sequencing Analysis: From A three day training program entitled “Next indigenous chicken of Manipur. for need the characterising and documenting about importance the chicken of local and awareness among breeders and apprise them collaboration with CAU, Imphal to generate 21.11.2014 at Keishamthong, Imphal in (W) of Manipuri Chicken’ was organized on One day workshop on ‘Characterization Training. overall India were participated in the January, 2015.Total 11Scientists from NBAGR orkshops orkshops th to 30 th th 4.

ICAR, Sh.ICAR, Anup Kumar Thakur,Secretary, Dr. S. Ayyappan, Secretary, DARE & DG, State (Agri & FPI) inaugurated meet. the Kumar Balyan, Hon’ble Union Minister of NASC Complex, New Dr.Delhi. Sanjeev 20 Productivity of Indigenous Cattle” on Meet on “Strategies for Enhancing Milk (NAVS) organized Consultation an Expert National Academy of Veterinary Sciences NBAGR incollaboration with ICAR and NBAGR. CFSP&TI and Director and Scientists from of SLDBs; from CHRS, CCBFs, Officials of Haryana; Directors of SAHDs; CEOs (DAHD&F),GOI; Principal Secretary Govt. and Asstt. Commissioner from DAHD&F, included Secretary, Joint Secretary, AHC attended by atotal of 56participants which attended consultation the meet. representatives from Gaushalas and NGOs Former CEOs, Directors, ADGs, DDGs, DAHDF, Dr. KMLPathak, DDG(AS), th October, 2014at A.P. Shinde Hall, jk-i-vk-la-C;wjks

71 ANNUAL REPORT 2014-15 TRAININGS AND WORKSHOPS ORGANIZED 72 ANNUAL REPORT 2014-15 TRAINING ATTENDED 5. 6.

jk-i-vk-la-C;wjks Resources of state Sikkim on 19 A brainstorming session on Genetic Animal Course Coordinators.Course Indrajit Ganguly and Sanjeev Singh were the Mukesh and Drs Monika RSKataria, Sodhi, course.this Director Course was DrManishi animal and science biotechnology attended & general universities working area inthe of institutes and SAUs) and other organizations participants from NAAS System (from ICAR from 01to 10December, 2014.Atotal of 21 of Indigenous Resources” Animal Genetic Approaches for Genome Characterization “Advanced Molecular and Bioinformatics Organized ICAR sponsored short course on officerssenior veterinary ofSikkim. Director, DAHLF&VS, Deputy Directors and The programme was attended by Additional Livestock Development Board, Gangtok. at Gangtok incollaboration with Sikkim TRAINING ANDCAPACITY BUILDING NBAGR th Nov, 2014 Officers of ICAR organized on26 on “Training Needs Assessment” for HRD Nodal Dr. Jaya Kumar, Scientist attended Trainings Attended Dr. Jaya Kumar, Scientist attended Karnal during 28-30 From Technology to Application" at NBAGR, entitled “Next Generation Sequencing Analysis: Ganguly attended athree day training program Drs. Monika Vikas Sodhi, Vohra and Indrajit 28,2014-Novemberber 17,2014. Food” Milk Designer at- NDRI Octo between Functional “Omics” Technologies for Future winter school on “Whole Genome Analysis and 2015 at NAARM, Hyderabad. December, 2014. Resources” at NBAGR 01to 10 between Characterization of Indigenous Animal Genetic Bioinformatics Approaches for Genome short on Course “Advanced Molecular and Dr. Jaya Kumar, Scientist attended 10days 1. indigenous animals: places to sensitize farmers about benefits the of Exhibitions on AnGR were organized at various Exhibitions Organized 2014 at NBAGR, Karnal. International Biodiversity Day, 22 th January, 2015. th February, February, Workshop 21days nd May May 2. TRAINING ANDCAPACITY BUILDING 3. 5. 4.

NBAGR Foundation Day 20 departments and stake holders on meet 18 ICAR Institutes-SAU- Development 2014 at NBAGR, Karnal. October 2014. October 2014atOctober NDRI, Karnal. Avikana At National sheep and wool fair at CSWRI Wheat Research, &Barley Karnal on 30 Rabi Kisan Mela held at Indian Institute of NBAGR gar, 12 November 2014. th September th th

9. 8. 7. 6.

Department Haryana on 13-15 March, 2015. Gurgaon,valley organized by Agriculture Agri Leadership Summit 2015 at leisure Institute, Karnal from 25-27February, 2015. MelaDairy held at National Research Dairy Mukatsar, Punjab, 8-12January, 2015. National Livestock championship held at November 2014 by Agriculture Department Haryana on 27 At NDRI Karnal Krishi Mela, organized jk-i-vk-la-C;wjks

73 ANNUAL REPORT 2014-15 EXHIBITIONS ORGANIZED 74 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics jk-i-vk-la-C;wjks NBAGR • DistinguishedVisitors • • Celebrations • ImportantMeetings • Library • Other Activities NBAGR Visits Abroad Swachh BharatAbhiyan jk-i-vk-la-C;wjks

75 ANNUAL REPORT 2014-15 jk-i-vk-la-C;wjks on & F u n cti a l G e no mics ati Ch aracteri z G e n etic granted. IBSC were permissions and reviewed necessary research of projects under purview falling the meetings on 02.07.2014and 30.01.2015.The involve biosafety guidelines, held its sixmonthly recombinant DNA work/transgenics, which projects of Bureau the engaged incloning/ NBAGR, constituted for monitoring research the The Institutional Biosafety Committee of IBSC Meeting held on 28 Institute research committee (IRC) meeting was IRC Meeting M eetings Important No. of News papers subscribed No. of Foreign journals subscribed No. of Indian Journals subscribed No. of added books Total readers. collection have for subscribed benefit the been scientificof foreign one journals and Indian thirty Journals 4111 Eleven procured during library inthe period. the and journals worth Rs.10,21,195/-wereBooks Bureau LAC was reconstituted on 19.5.2014. purchase of etc.books/journals scientific The management of issues library the to pertaining Committee (LAC) is guiding force inthe stafftechnical the of Bureau. Library Advisory important scientists the role and in serving T Library final final report of completed projects. projects was the reviewed and discussed also NBAGR. Progress report of on-going research Chairmanship of Dr. Arjava Sharma, Director OTHER ACTIVITIES h e NBAGR playing has library been an NBAGR th & 29 & th November, 2014under the 07 11 31 114 planted Bureau inthe campus. 400 flowering, non-floweringfruit and trees were Forest occasion. Around the Department graced out. Sh. Navdeep Hooda, Conservator, Haryana drive to plant trees campus inthe was carried to NBAGR and occasion, aspecial On this ICAR. upon scientists the called to bring more laurels highlighted activities of the Bureau the and on 16.07.2014.Dr. Arjava Sharma, Director August 2014 and Republic Day on 26 NBAGR office building. trees were planted infront of and around ICAR- the Around 200flowering, non-flowering fruit and Yadav, Principal Scientist coordinated function. the andlogistics plant saplings. Dr. Dinesh Kumar was guest the on occasion the and provided all over Sh. function. the Naresh DFO, Ranga, Karnal Arjava Sharma, Director, ICAR-NBAGR presided Diwas” Karnal celebrated “Taru ICAR-NBAGR and Haryana Forest Department, Taru Diwas Bureau celebrated Independence the Day on 15 DayIndependence &Republic Day Celebrations The 86 ICAR Foundation Day prizes were distributed to participants. the of staff presentedculturalthe programme. The functionsthe and hoisted tricolor. the The wards 2015 at office campus. Director, presided over th ICAR foundation day was celebrated on 15.07.2014. Dr. jk-i-vk-la-C;wjks th January, th

77 ANNUAL REPORT 2014-15 OTHER ACTIVITIES 78 ANNUAL REPORT 2014-15 OTHER ACTIVITIES certificates and shields. of competitions the were awarded suitably with poster presentation competition. Thewinners The studentsparticipated enthusiasticallythe in understand about of activities the institute. the wherein visited they various laboratories to kept day' as 'Open for outside the students, opportunities inbiotechnology. Theday was resources of and on country the also career to make students aware about livestock genetic Director NBAGR on Farm Animal Biodiversity on biodiversity. Lectures were delivered by for Nation Building' and a poster competition competitionlike speech on theme the 'Science participated event, inthe marked by activities and teachers of five different degree colleges undergraduate and post-graduate students Day' on February, 28th 2015.More than 80 The Bureau celebrated 'National Science National Day Science create awareness . premises on National the Highway (NH-1)to by bureau stafffrontalso wasformed in of bureau chain causethis of nationalhuman importance. A appealed to contribute at least 100hours inayear to staffEvery activities. member the of Bureau was monthof ear-marked every has been for such bureau staffparticipated actively.Second Saturday cleanliness campaign was initiated, where the inall As of part "Swachh Bharat Abhiyan" acampus Swachh BharatAbhiyan jk-i-vk-la-C;wjks NBAGR scientists. Bureauthe during year the and interacted with Following distinguished personalities visited Distinguished Visitors OTHER ACTIVITIES 7. 6. 5. 4. 3. 2. 1. OTHER ACTIVITIES

Bureau on 04.02.2015. ILRI v Director General,Dr. Jimmy Smith, 25.11.2014. (CARD), Noida visited NBAGR, Karnal on for Agriculture and Rural Development PNB Progressive Farmers from Centre Karnal on 15.11.2014. Channel}and visited NDTV the NBAGR, Journalist {Hindustan Times Newspaper Livestock and a well Expert known Sharma,Sh. Devinder Agriculture and Manipur, visited Bureau the on 15.09.2014. (NEBADO) Kasom Sub Khillen Division, Border Area Development Organization Mr. Paisho Keishing from North East visited NBAGR, Karnal on 29.08.2014. U.S. of Library Congress, American Center Ms. Manjula Librarian, Chawla, Serials Scientist visited Bureau the on 23.08.2014. Component-1 and Dr. P. Katihaa, Principal Dr. P. S. Pandey, National Coordinator, National Coordinator, Component-4, by NAIP Team consisting of Dr. S.Kochhar, Terasawa, World accompanied Experts Bank Mohinder S.Mudahar and Dr. Miki The World Team Bank consisting of Dr. visited on 25.04.2014. National Development Dairy Board, Anand Dr. NBAGR R Kasiraj, Manager General (AB), isited Various Laboratories of the 8. 10. 9. Expert Lecturer.Expert IAEA-sponsored training capacity inthe of training to international the participants of (Austria) during August 9-17,2014to impart Dr. Arjava Sharma, Director, visited Vienna Visits Abroad 11.

ASRB visited us on 05.02.2015. Dr. Gurbachan Singh, Chairman, Karnal on 26.02.2015. Biotechnology, Bangladesh visited NBAGR, A team of scientists from National Institute of 07.02.2015, to aquainted with Bureau activities. Uttar Pradesh visited NBAGR, the Karnal on State Progressive farmers from Baiti village, Bureau on 27.02.2015. Biotechnology, Govt. of India visited the and Dr. SR Rao, Advisor, Senior Dept. of Agricultural Specialists, USDA, New Delhi Kumar Singh and Dr. Vijay Intodia, Lagos, Agricultural Attache, Dr. Santosh USDA,Service, Washington; Mr. Joshua Methods Division, Foreign Agricultural Advisor, New Technologies and Production Dia Dr. ne Wray-Cohen, Science Senior jk-i-vk-la-C;wjks

79 ANNUAL REPORT 2014-15 OTHER ACTIVITIES jk-i-vk-la-C;wjks NBAGR on & F u n cti a l G e no mics ati Ch aracteri z G e n etic Superannuation • Joinings/Transfers • Promotions • BureauStaff • Personnel NBAGR jk-i-vk-la-C;wjks

81 ANNUAL REPORT 2014-15 jk-i-vk-la-C;wjks NBAGR on & F u n cti a l G e no mics ati Ch aracteri z G e n etic Technical Staff Scientific Staff (ason31.03.2015) Staff Bureau PERSONNEL 5. 4. 3. 2. 1. No. Sl. 29. 28. 27. 26. 25. 24. 23. 22. 21. 20. 19. 18. 17. 16. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. No. Sl. NBAGR Sh. Harvinder Singh MathurSanjeevSh. Dr. P.S. Panwar Sh. S.K.Jain Dr. P. S.Dangi Name Dr. Ahlawat Sonika Dr. Jayakumar S. Dr. K.N.Raja Dr. Karan Veer Singh Dr. P. Kathiravan Dr. Sanjeev Singh Dr. Indrajit Ganguly Dr. Saket Kr. Niranjan Dr. Vikas Vohra Dr. Sharma Rekha Dr. Behl Rahul Dr. Avnish Kumar Dr. Arora Reena Dr. Manishi Mukesh Dr. Dinesh Kumar Yadav Dr. Satpal Dixit Dr. Jyostna Behl Dr. Monika Sodhi Dr. Kumar Anil Mishra Dr. Kataria R.S. Dr. Pundir R.K. Dr. P.K. Singh Dr. Aggarwal R.A.K. Dr. N.K.Verma Dr. P.K. Vij Dr. M.S.Tantia Dr. Anand Jain Dr. Vijh R.K. Dr. Arjava Sharma Name of Scientist Scientist Scientist Scientist (S.S.) Scientist (S.S.) ScientistSenior ScientistSenior ScientistSenior ScientistSenior ScientistSenior ScientistSenior ScientistSenior Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Principal Scientist Director Designation Senior TechnicalSenior Officer TechnicalSenior Officer TechnicalSenior Officer Asstt. Technical Chief Officer Asstt. Technical Chief Officer New Designation jk-i-vk-la-C;wjks

83 ANNUAL REPORT 2014-15 PERSONNEL 84 ANNUAL REPORT 2014-15 PERSONNEL Administrative Staff Skilled Support Staff Skilled Support 5. 4. 3. 2. 17. 16. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. No. Sl. 1. 19. 18. 17. 16. 15. 14. 13. 12. 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. S. No. jk-i-vk-la-C;wjks Sh. Babu Ram Sh. Naresh Kumar Smt. Neerja Kaul Sh. Rajnish Kumar Sh. Shiv Chander Sh. Satish Kumar Sh. Sopal Sh. Yoginder Sh. Jita Ram Smt. Shashi Bala Smt. Indu Steno Bala, Smt. Amita Kumari Smt. Anita Chanda Sh. Behl Ramesh Sh. Pawan Kr. Gupta SinghSh. Balkar Sh. Karambir Sh. Sunil Kumar Sh. Jagtar Singh Name Sh. Singh Balvinder Sh. Chand Ramesh Sh. OmPrakash Sh. Mahavir Singh Sh. Ashok Kumar Sh. Subhash Chander KumarSh. Rakesh Sh. Kumar Ramesh Sh. Naresh Kumar Smt. Pravesh Kumari Sh. Jamer Singh Sh. Sat Pal Name Sh. Satbir Sh. Deepak Sh. Deepak Sh. Ram SagarSh. Ram Sh. Sewa Ram Ram Sh. Sewa Sh. Krishan Lal NBAGR Technician TechnicalSenior Assistant TechnicalSenior Assistant TechnicalSenior Assistant TechnicalSenior Assistant TechnicalSenior Assistant TechnicalSenior Assistant Technical Officer Technical Officer Technical Officer Technical Officer Technical Officer New Designation SSS SSS SSS SSS SSS LDC LDC LDC LDC LDC UDC UDC Assistant Assistant Assistant Steno Gr.III PA PA Assistant AF&AO AAO PS to Director F&AO Admn. Officer Designation PERSONNEL 11. 10. 9. 8. 7. 6. 5. 4. 3. 2. 1. Promotions PERSONNEL

w.e.f. 16.12.2014. Upgradation of Rs.5200-20200+2000/- GP Sh. Satbir, granted SSS has been Financial 20200+2000/- GPw.e.f. 01.11.2014. Financial Upgradation of Rs.5200- granted SSShas Rai, been Sh. Deepak w.e.f. 01.11.2014. Upgradation of Rs.5200-20200+2000/- GP Sagar,Sh. Ram granted SSShas been Financial Technical Officer w. e. f.14.05.2014. promoted toof next grade the higher Senior Sh. Harvinder, Technicalbeen Officer has 16.09.2013. of grade higher Technical Officer w. e. f. Assistant promoted has been to next the Sh. Kumar, Ramesh Technical Senior 37400-67000+RGP 9000/-w.e.f. 29.06.2014 promoted of to next grade the higher Rs. Dr. Sanjeev Singh, Scientist Senior has been 21.04.2014 of Rs.37400-67000+RGP 9000/-w.e.f. promotedbeen to next grade the higher Dr. Indrajit Ganguly, Scientist Senior has 37400-67000+RGP 9000/-w.e.f. 04.04.2014 promoted of to next grade the higher Rs. Dr. S.K.Niranjan, Scientist Senior has been 37400-67000+RGP 9000/-w.e.f. 02.04.2014 promoted of to next grade the higher Rs. Dr. Vikas Vohra, Scientist Senior has been dated 28.04.2014. ICARvide O.O. dated O.O. 21.04.14&this 15600-39100+RGP 7000/-w.e.f. 06.01.2012 promoted of to next grade the higher Rs. Dr. Dr. Scientist K.N.Raja, (SS)has been dated 15.02.2014. ICAR O.O. dated O.O. 02.04.2014&this Principal Scientist w.e.f. 09.09.2012vide promotedbeen to of next the post higher Dr. Manishi Mukesh, National Fellow has NBAGR Superannuation 1. Joinings/Transfer 2. 1.

CIRC Meerut. NBAGR, Karnal on 14.08.2014(AN)to join CIRC, Meerut. He was relieved from ICAR- forwas selcted of post the Director, ICAR- Dr. Birham Prakash, Principal Scientist superannuation from ICAR on 31.12.2014 Dr. D. K.Sadana, Principal Scientist attained superannuation from ICAR on 30.06.2014 Sh. Moti Technical Ram, Officer attained jk-i-vk-la-C;wjks

85 ANNUAL REPORT 2014-15 PERSONNEL 86 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics jk-i-vk-la-C;wjks NBAGR • • • fgUnh [k.M NBAGR fgUnh vuqHkkxdhxfrfof/k;k¡ dk;Zdkjh lkjka'k izkDdFku jk-i-vk-la-C;wjks

87 ANNUAL REPORT 2014-15 88 ANNUAL REPORT 2014-15 Genetic Characterization & Functional Genomics jk-i-vk-la-C;wjks NBAGR ds varxZrfd;ktkjgkgSA ifj;kstuk usVodZ dk;Z laj{k.k dk eqfxZ;ksa gfju?kVk vkSj xk; vksaxksy ,oa ckjxqj rFkk y{k.khdj.k dk cÙk[kksa dh vklke eqxhZ] gktjk x/kksa] ds jktLFkku feFkqu] rFkk ;kd v#ukpyh Å¡V] tkyksjh 'kwdj] ds vklke cdjh] Hkkdjoky rFkk cqUnsy[kaMh HksM+] vleh rFkk dkslyh xk;] ekjokMh] iw¡Nh vkSj frCcrh fcU>jiqjh] iwf.kZ;k] gSaA 'kkfey ykbu isjsaV esy dh eqfxZ;ksa (oujktk) ihMh1 vkSj eqxhZ esokM+h 'kwdj] xksouk vxksank Å¡V] [kjkbZ cdjh] iartk HksM+] CySd x;k gSA buesa fcykgh rFkk xaxkrhjh xk;] dpbZdVh fd;k }kjk C;wjks Hkh iathdj.k dk ykbu isjsaV esy ,d dh eqxhZ rFkk uLyksa u;h lkr dh eqfxZ;ksa vkSj esa mi;ksx fd;k x;k gSA bl vof/k ds nkSjku i'kqvksa {ks= çtuu blds oh;Z dk HkSal tkQjkcknh fy, ds dk;Z fodkl vkSj laj{k.k gSA x;k fd;k 'kkfey dks Fkkjikjdj) rFkk VksMk HkSal dh 7600 oh;Z [kqjkdksa vkSj (xkvksykvks xksoa'k varxZr ds dk;ZØe laj{k.k ,Dl&lhVw esa cSad thu ds C;wjks gSA ij çxfr dk;Z dk fu/kkZj.k y{k.k ds eqxhZ dksuk;su vkSj cdjh flfDde HksM] ;sYxk ,oa ekÅyh xk;] lhjh gSA x;k fd;k iwjk dks dk;Z fuèkkZj.k y{k.k çk#ih ds vkcknh de&Kkr dh i'kqvksa nslh lkFk&lkFk ds 'oku fpIihijbZ vkSj jktiy;e cdjh] HksM+] fla?kkjh dtyh vuqlkj ds tukns'k nkSjku ds o"kZ bl gSA ç;kljr fujarj fy, ds djus igpku dh {kerkvksa vkuqoaf'kd rFkk fo'ks"krkvksa fofHkUu dh ls ckn lalkèkuksa vuqokaf'kd ds laink ds i'kq Hkkjrh; laLFkku LFkkiuk viuh esa 1984 o"kZ gSA jgk gks g"kZ vikj eq>s gq, djrs çLrqr çfrosnu okf"kZd dk 2014&15 ;qä ls miyfC/k;ksa vkSj jk"Vªh; i'kq vkuqoaf'kd lalk/ku C;wjks dh xfrfof/k;ksa fgUnh [k.M NBAGR izkDdFku ls vk;kstu fd;k x;kA Hkkjrh; Ïf"k vuqlU/kku Ïf"k Hkkjrh; x;kA fd;k vk;kstu ls :i fu;fer dk cSBdksa vkbZ-vkj-lh- esa laLFkku dk;Z vkjEHkfd;kgSA dk yxkus irk dk chekfj;ksa oa'kkuqxr esa xksi'kqvksa vkSj j[kk tkjh dk;Z dk djus çnku lsok,a ijke'kZ fy, ds LØhfuax lkbVkstsusfVd dh lkaMksa çtud us laLFkku vfrfjä] blds x;kA fd;k fy, ds djus iSnk dh xbZ] ftldk mi;ksx czhM flXuspj fodflr tkudkjh ij i'kqvksa QkeZ 541 ds lkghoky) vkSj Fkkjikjdj] (fxj] uLyksa xksoa'k 3 djds mi;ksx dk fpUºdksa ,l-Vh-vkj- 4 x;kA fd;k fu/kkZj.k y{k.k dk thuksa çfrHkkxh fofHkUu vkfn) 147 fdl&1 vkfn) vkSj vuqdwyu thu (,elhVh] lhMh vkfn) iqumZRiknu thu (thMh,Q&9] ch,eih&15] ,ufdfju VhVhu] lh,,lVh] lh,ih,u&1] ihvkbZVh&1] ysfIVu] th,pvkj] th,p] Mhth,Vh&1] (es;kslVkVhu] thu fy, ds mRiknu esa uLyksa nslh xbZA ns[kh vuqifLFkfr yxHkx dh çHkko ds uLyksa fons'kh esa xk;ksa yík[kh fodflr ls :i LokHkkfod esa thuksa çfrHkkxh lEcaf/kr ls fo'ks"krkvksa dh nw/k gSA ij çxfr dk;Z dk fu/kkZj.k y{k.k vkuqoaf'kd ds HkSalksa NÙkhlx<+ vkSj vksfM'kk xbZA dh igpku dh mifLFkfr dh fofo/krk vkuqoaf'kd mPp esa HksM+ksa blds vfrfjä] vk.kfod y{k.k fu/kkZj.k ls dksjkiqV jk-i-vk-la-C;wjks

89 ANNUAL REPORT 2014-15 izkDdFku 90 ANNUAL REPORT 2014-15 izkDdFku Mh a; ea aFk d nSk dk te MkW- ftues fd;k nkSjk dk laLFkku esa la[;k cM+h us O;fä;ksa x.kekU; dbZ vfrfjä] blds nkSjk fd;kA dk vkfLVª;k fo;suk] fy, ds nsus çf'k{k.k dks çfrHkkfx;ksa varjkZ"Vªh; ij] rkSj ds O;k[;krk fo'ks"kK eSaus] x;kA fd;k vk;kstu dk n`f"Vdks.kÞ foKku lwpuk tSo vkSj vk.kfod mUur fy, ds fo'ks"krk thukse dh lalk/kuksa vkuqoaf'kd i'kq ßnslh l= çf'k{k.k laf{kIr ,d çk;ksftr }kjk ifj"kn~ kku vuqlU/ Ïf"k Hkkjrh; x;kA fd;k vk;kstu dk l= jkT; ds i'kq vuqokaf'kd lalk/kuksa ij ,d cqf¼'khyrk i'kq/ku fodkl cksMZ] xaxVksd ds lg;ksx ls flfDde flfDde xbZA dh iSnk tkx#drk chp ds turk ls ek/;e ds O;k[;kuksa vkSj l=ksa eaFku çn'kZfu;ksa] fd;k x;kA laLFkku }kjk i'kq vkuqoaf'kd laink ij vk;kstu dk dk;Z'kkyk ijke'kZ fo'ks"kK ,d ij fo"k; j.kuhfr;kaÞ fy, ds c<+kus mRikndrk nw/k esa i'kqvksa ßnslh ls lg;ksx ds ,u-,-oh- vkSj ifj"kn~ jk-i-vk-la-C;wjks NBAGR lq>koksa dkLokxrgSA vkids fy, ds ekxZn'kZu vkSj lq/kkj gw¡A nsrk c/kkbZ çfrosnu iw.kZ gqvk gS vkSj ç'kklfud vf/kdkfj;ksa dks okf"kZd ;g ls ;ksxnku ftuds rduhdh oSKkfudksa] lHkh eSa ls Hkko xkSjokfUor gw¡A vkHkkjh cgqr fy, lexz fodkl gsrq yxkrkj leFkZu vkSj ekxZn'kZu ds ds laLFkku bl dk oSKkfud ç/kku Hklhu] fouhr MkW- rFkk iz-) ,oa (i-m- egkfuns'kd lgk;d xk¡/kh] vkj-,l- MkW- foKku)] (i'kq egkfuns'kd mi ikBd] ds-,e-,y- MkW- ifj"kn~] vuqlU/kku Ïf"k Hkkjrh; eS] MkW- ,l- v;IIku] lfpo (Ms;j) vkSj egkfuns'kd] çeq[k gSaA (dhfu;k) uSjksch byjh] egkfuns'kd] fLeFk] fteh fgUnh [k.M (vktZo 'kekZ) funs'kd dh dpbZdVh cySd HksaM+_ xqtjkr dh [kjkbZ Å¡V] [kjkbZ dh xqtjkr HksaM+_ cySd dpbZdVh dh rfeyukMq cdjh_ iartk dh mŒizŒ ,oa mÙkjk[kaM xk;_ xaxkrhjh dh fcgkj ,oa mŒizŒ xk;_ fcykgh dh paM+hx<+ ,oa gfj;k.kk esa uLyksa iathd`r xbZA dh iathd`r }kjk laLFkku ckj igyh dh ykbu isjsaV esy dh eqxhZ ,d FkhA uLy ,d dh izR;sd eqxhZ ,oa ftlesa 2 uLysa xk;ksa dh ,oa HksaM+] cdjh] 'kwdj] Å¡V x;k] fd;k iathdj.k dk uLyksa ubZ 7 dh i'kqèku # FkktcfdizkIrjktLo21-68yk[kjgkA gq,A o"kZ ds nkSjku jktLo izkfIr dk y{; 22 yk[k ifj;kstuk ds vUrZxr dqy # 178-72 yk[k [kpZ usVodZ gqvkA O;; #i;s yk[k 1179-332 ls esa yk[k 1186-00 #i;s izkIr dqy rgr ds ;kstuk vkSj ;kstuk xSj nkSjku ds 2014&15 o"kZ foÙkh;     C;wjks fuEumís';ksadhizkfIrgsrqdk;ZjrgS% gSA laLFkk lrr vxz.kh ,d dh ns'k muds gsrq vfèkns'k ds mi;ksx vkSj laj{k.k fuèkkZj.k] xq.k ewY;kadu] igpku] dh lalkèkuksa dqDdqV o i'kq ikyrw esa Hkkjr C;wjks ;g gqvkA esa :i ds C;wjks lalkèku vkuqoaf'kd i'kq jk"Vªh; foy; dk laLFkkuksa nksuksa bu esa 1995 o"kZ x;kA fn;k dj LFkkukarfjr dSEil edjeiqj orZeku bls esa 1994 o"kZ x;kA fd;k LFkkukUrfjr ls :i vLFkkbZ esa ifjlj djuky jk-Ms-v-la- dks laLFkku rFkk C;wjks flrEcj] esa 1985 21 o"kZ x,A fnukad fd;s LFkkfir dks esa 1984 caxykSj dsUnz {ks=h; ds laLFkku vuqlaèkku Msjh jk"Vªh; laLFkku] vkuqoaf'kdh j dk;Zdkjh lkjka'k fgUnh [k.M d lalkèku C;wjks vkSj jk"Vªh; i'kq i'kq jk"Vªh; vkSj C;wjks lalkèku vkuqoaf'kd i'kq k"Vªh; dk;ZØeksa dklapkyudjukA izf'k{k.k] lacafèkr ls mi;ksx vkSj fuèkkZj.k xq.k i'kq o dqDdqV vkuqoaf'kd lalkèkuksa ds ewY;kadu] djukA vè;;u ij fuèkkZj.k xq.k vkuqoaf'kd ls iz;ksx vk.kfod tho foKku dh vkèkqfud rduhdksa ds fy, rduhdksadkfodkldjukA ds izcaèku bu&lhVw mi;ksx] rFkk laj{k.k loksZÙke ,Dl&lhVw ds laink vkuqoaf'kd i'kq jk"Vªh; vkadM+kdks"kdhLFkkiukdjukA vkSj lapkyu dk losZ{k.kksa O;ofLFkr fy, djus ds lwphc¼ rFkk ewY;kadu fuèkkZj.k] xq.k ds lalkèkuksa vkuqoaf'kd dqDdqV o i'kq ikyrw NBAGR (,DlVªhfeVht) lQsn gksrs gSaA FkwFku dkyh&Hkwjh ;k dkyh&Hkwjh FkwFku gSaA gksrs lQsn (,DlVªhfeVht) Nksj vafre lHkh ds 'kjhj vfrfjDr ds dku ,oa vkdkj ds gksrs gSa tks fd iw.kZ dkyk rd gksrs gSaA psgjs fofHkUu èkCcs lQsn ;qDr èkCck esa jax Hkwjs ;k dkyk lQsn] psgjk gSA gksrk ekStwn xqPNk dk cky esa {ks= tk¡?k gSaA gksrs cky ?kus NksVs ij ftl gS gksrk lQsn jax dk [kky gSA gksrh dh vkdkj eè;e HksaM+ ;yxk gksrs gSaA jfgr lhax nksuksa eknk ,oa uj gSA gksrk lhèkk ls :i dk vkdkj BsB jkseu izdkj dk gksrk gS ;k rqyukRed ukd buds x,A ik, ds izdkj eM~x;ky BsB lewg dqN gSA tkrh ikbZ xksykbZ dh jax Hkwjs rjQ pkjksa ds vk¡[kksa gSaA gksrs Hkh ds èkCcs fcuk vkSj gSa tkrs ik, Hkh gSA HksaM+ dk jax lQsn gksrk gSa ftlij Hkwjs jax ds èkCcs gksrh vfèkd Å¡pkbZ dh tkuojksa ,oa xgjk 'kjhj gSa] gksrs yEcs iSj buds gSA tkrh ikbZ vfèkd fofHkUurk buesa ysfdu gS] gksrh okyh vkdkj 'kkjhfjd HksaM+ cM+s ekÅyh x,A fd;s ,d= vkadM+s lacaèkh 'kjhj Hkkj ,oa eki 'kkjhfjd x;kA fd;k losZ{k.k vè;;u gsrq ds HksaM+ ;yxk ,oa ekÅyh dh dukZVd fouez gksrkgSA yxHkx tehu dks Nw jgh gksrh gSA Fku NksVk gksrk gSA LoHkko tks gS gksrh yEch iawN gaSA gksrs ds vkdkj eè;e ,oa NksVs dku gSaA gksrs NksVs xydEcy ,oa lQsn èkCcks ;qDr gksrk gSA dku lhax eqMsa+ ,oa gq, Hkwjk gksrs gSaA dkyk] dwcM+ jax dk 'kjhj ds xk;ksa x;kA dk losZ{k.k ds ekè;e ls n`';#ih y{k.khdj.k fd;k Å¡pkbZ ij j[kus gsrq vH;Lr yn~nk[kh xksoa'kh i'kqvksa ,dM+ [ksrtksrktkrkgSaA ,d yxHkx esa ?kaVs 8 ls 6 ls cSyksa tksM+h ,d gSA gksrk fnu izfr fdxzk- 5 ls 2 mRiknu nqXèk gSaA gksrs NksVs Fku gSA gksrh yEch rd V[kus iwaN gSaA gksrs NksVs dku gSaA tkrs ik, Hkh èkCcs ds jax lQsn esa jax Hkwjs fd;k x;kA buds 'kjhj dk jax Hkwjs ls dkyk gksrk gS] 'kkjhfjd fo'ks"krk,a] mRiknu Lrj vkfn dk vè;;u eki] 'kkjhfjd mudk fyax] ,oa mez fofHkUu fd tks xksoa'k] 68 dqy x;kA fd;k fuèkkZj.k xq.k }kjk ds losZ{k.k dk ßlhjhÞ uLy xksoa'kh ns'kh dh flfDde rd iathd`r dqy uLyksa dh laLFkk 151 gks xbZ gSA vc esa ns'k izdkj bl FkhA uLysa dh eqxhZ esokM+h dh jktLFkku vkSj uLy lqvj xksou vxks.Mk dh xksok jk-i-vk-la-C;wjks

91 ANNUAL REPORT 2014-15 dk;Zdkjh lkjka'k 92 ANNUAL REPORT 2014-15 dk;Zdkjh lkjka'k ds gksrs gSaA i`"B Hkkx ij dkys jax dh js[kk (dkyh js[kk dh jax dkys ij Hkkx i`"B gSaA jax gksrs ds lQsn ;k dkys eè;e ,oa gSA etcwr] NksVs] gksrk iSj dk jax lQsn ;k Hkwjk gYdk lkekU;r% fgLlk fupyk dk isV gSaA gksrs yVdnkj vèkZ ,oa gS gksrk xksykdkj fljk ftudk gSa gksrs ds vkdkj eè;e ,oa NksVs dku gSaA gksrh NksVh ,oa pednkj okyh tkus èkkfj;ksa }kjk vklkuh ls ikbZ igpkuk tk ldrk gSA vka[ks ij psgjs buds bUgsa ,oa gS ?kVd izeq[k dk cdfj;ksa dqy dh flfDde fla?kkjh] x;kA fd;k losZ{k.k fy, ds djus fuèkkZj.k xq.k dk cdjh dkyh flfDde ,oa fla?kkjh % lewg cdjh flfDde ftudk dqyoSfj,Ul66-3ls71izfr'krFkkA ,oa fd;k x;kA ih-lh-,- us nks ?kVdks dks ,DLVªsDV fd;k lksykiqjh (ih-lh-,-) fo'ys"k.k ?kVd eMx;ky] fizalhiy ij dksYgkiqjh iz;ksx] dk lacaf/kr) 14 'kkjhfjd fo'ks"krkvksa lacaèkh uLyksa (ekQksZehfVªd bdksVkbIl dks 5 fofHkUu oxksZa esa oxhZd`r fd;k x;kA ikapks bu us fo'ys"k.k fMfLØfeusUV x;kA fd;k kjk } fofèk losZ{k.k vkèkkfjr lk{kkRdkj laxzg.k] dk vkadM+ks lacafèkr tuu ,oa mRiknu muds ls {ks= dh dksYgkiqjh) fo'ks"krkvksa dk vè;;u djus ds ,oa fy,] muds forj.k yksukaM laxeusjh] lksykiqjh] (eM~x;ky] bdksVkbIl ikapksa dh uLy HksaM+ MDduh 1000 xzkegksrkgSA ls 800 mRiknu Åu vkSlr gSaA gksrs jfgr lhax eknk ,oa uj gSA gksrh jgh Nw rd tehu iwaN esa dqN dh] yEcs ,oa yVdrs dku ,oa iwaN Hkh yEch gksrh gSA lqxfBr ,oa cM+s vkdkj dk 'kjhj gksrk gSa] dk ukd jkseu HksM+ksa izdkj gSA gksrk dk Lrj fofHkUu ij dku ,oa psgjs forj.k ftudk gSaA tkrs ik, èkCcs ds jax gksrk gS ysfdu psgjs ,oa dku ij dkys ;k dkys&Hkwjs gSaA lQsn (fpV~Vh) dtyh% ftldk iwjk 'kjhj&lQsn gksrk lQsn izfr'kr) 41-57 (yxHkx fljk vafre dk iwaN ftudh rFkk gS gksrk dk jax Hkwjs & dkys ;k fd;k x;k gSA dkyh dtyh% bldk iwjk 'kjhj dkyk ij vkèkkj ds jax 'kkjhfjd muds oxhZdj.k ftudk fdxzk- gksrk gSA bldh nks fdLesa iatkc esa ikbZ tkrh gSa Hkkj Øe'k% 56-98$ 1-02 ,oa 43-23$ 0-36 fT+kyksa esa ik;h tkrh gSA o;Ld uj ,oa eknk dk 'kjhj lax:j] cjukyk] yqfèk;kuk] eksxk ,oa vkl&ikl ds dtyh HksaM+ dk losZ{k.k fd;k x;kA ;g HksaM+ iatkc ds tkrk gSAujlhax;qDr,oaeknklhaxjfgrgksrh xqykch jax dh gksrh gSA oSVYl lHkh tkuojksa esa ik;k jk-i-vk-la-C;wjks NBAGR ykslkbZ dk mi;ksx djds 541 QkeZ i'kqvksa dh 3 dh i'kqvksa QkeZ 541 djds mi;ksx dk ykslkbZ uLy gLrk{kj fodflr djus ds fy, 4 ,l-Vh-vkj- gSaA vkSlrvar%iztuuxq.kkad 0-037FkkA jgs n'kkZ dks fofHkUurk vkuqoaf'kd i;kZIr (0-79)] gsVjkstkbZxksflVh izkIr (4-84)] la[;k dh ,yhyksa 9-925 ikbZ xbZ ,yhyksa dh la[;k (9-95)] izHkkoh yksdl izfr la[;k vkSlr ftudh x,] ik, ,yhYl 200 dqy x;kA fd;k laxzfgr vkadM+k vkèkkfjr ekbØkslSVsykbV gq, djrs mi;ksx dk fpUgdksa 20 yn~nk[kh xk;ksa dh vkuqoaf'kd fofHkUurk dk vè;;u f'kdkj ;k'kkSddsfy,ikyrsgSaA ;k j[kokyh bUgsa yksx gSA gksrh eghus 12&16 mez voLFkk esa Hkh ik, tkrs gSaA budh ;kSu ifjiDork dh vkdkj ds ,oa lhèks lery gksrs gSaA dqN ds dku [kM+h (QkWu) ls xgjk Hkwjk vkSj dkyk gksrk gSA dku eè;e [kkdh gYds jax ftudk dqÙks gSa gksrs okys vkdkj fpIihijkbZ eè;e gSA tkrh ikbZ esa fT+kyksa FkwFkwdqMh ,oa fo:nèkuxj enqjS] fr:usyoyh] ds rfeyukMq fpIihijbZ 'oku dk losZ{k.k fd;k x;k tks fd nf{k.kh jax dkgksrkgSA 42-43 otu vkSlr ftldk gS gksrk gksrh dk vkdkj eè;e v.Mk gSaA dh fdLe yM+kdw ;g gSA gksrk 45 ls 35 mRiknu v.Mk okf"kZd gSA gksrk fdxzk- 3 ls 2-5 yxHkx Hkkj 'kjhj gSA gksrh dh dkWEc) (ih izdkj ds ik, tkrs gSaA dyxh yky jax dh gksrh gS tks fd eVj èkCcs ds jax lqugjh ;k dkys lQsn] ftuij gS gksrk losZ{k.k fd;k x;kA buds ia[k dk jax dkyk ;k Hkwjk dk fT+kyksa if'peh bEQky ,oa FkkScky fy, ds djus ef.kiqj dh LFkkuh; ßdkSuk,uÞ eqxhZ dk xq.k fuèkkZj.k ekal dsfy,ikyhtkrhgSaA ls FkksM+h NksVh gksrh gSaA flfDde dkyh cdjh eq[;r% fla?kkjh esa vkdkj cdfj;k¡ ;s gSaA gksrs cMs+ esa rqyuk dh cdjh fla?kkjh iwaN] ,oa lhax psgjk] gSA gksrk esa vuqikr ds 'kjhj flj] gSaA gksrh dh vkdkj eè;e cdjh dkyh flfDde gSaA gksrh fHkUu ij vkèkkj ds vkdkj Hkh ls uLy caxky CySd vkSj gSa] gksrh fHkUu [kky ds jax] lhax ds izdkj ,oa vkdkj ds vkèkkj ij muds ls cdjh fla?kkjh cdfj;ksa] ;s gSaA gksrh dh jax ds feJ.k rhuksa bu ;k lQsn dkyh] Hkwjh] tksfd dk cdfj;ksa ,slh gS gksrk feJ.k lewg cdjh flfDde gSA tkrh ikbZ esa cdfj;ksa lHkh yxHkx ykbu) VkWi ± 0-07 xzke gksrk gSA v.Mk Hkwjs v.Mk gSA gksrk xzke 0-07 fgUnh [k.M

gSaA MhD;wch ds fy, dqy 16 ,yhyl ds rhu izeq[k ,yhy) vkSj MhD;w, 2 (2,yhy) ds fy, mRrjnk;h dh ,yhy (11 MhD;w,A lewgksa] cM+s nks fd tks xbZ dh igpku MhD;w, 13 dqy esa HkSalksa nynyh; ,DlkWu 40dkscgq:ihik;kx;kA ds thu ,Q,,l,u dsoy x;kA fd;k vè;;u dk fHkUurk esa HkSal NRrhlx<+h dj mi;ksx dk rduhd ihlhvkj&vkj,Q,yih Hkh ls ;qVhvkj 3* 1ds Vh (,DlkWu 38] 39] 40] 41] 42) vkSj ,l Vh , ,Q,,l,u thu mEehnokj okys djus izHkkfor dks lkFk unh; Vkbi dk irk pykA olk mRiknu y{k.k ds lkbZaVkstsusfVd fo'ys"k.k ls xq.klw= la[;k 50 ds uewuksa ds HkSal NRrhlx<+ x,A fd, ntZ eki nSfgd fd;k ij HkSal NRrhlx<+h o;Ld 140 yxHkx vkSj x;k fuèkkZj.k y{k.k iz:ih x;kA fd;k losZ{k.k ik;yV esa xkaoksa 41 ds fT+kyksa cLrj vkSj dkoèkkZ fcykliqj] egkleqan] dkadsj] èkerjh] ds NRrhlx<+ lkFk lewghdj.kdkirkpykA ds uLyksa HkSal unh; vU; dk vkcknh HkSal mM+hlk ls fo'ys"k.k usVodZ tks;fuax ehfM;u vkèkkfjr ij 57 gsiyksVkbZIl dk irk pykA gsiyksVkbZIl 'ks;fjax esa uLyksa@vkckfn;ksa nynyh; vkSj unh; ukS djds fof'k"V fo'ys"k.k rqyukRed pykA irk dk gksusa budk HkSal unh; ls vuqØe.k vkSj izoèkZu ds {ks= Mh&ywi ekbVksdkWfUMª;y chih 811 ds HkSalksa 19 vkSj 30 Øe'k% fy, ds vè;;u ds lkbZVkstsusfVd HkSalksa ijy[keq.Mh vkSj dkykgkaMh dh mM+hlk fd;k x;kA ekU; fy, ds bLrseky ij uLyksa i'kq Fkkjikjdj fdV dk ijh{k.k fd;k x;k rFkk lkghoky] fxj vkSj x;kA fd;k fy, ds djus fodflr fdV ihlhvkj eYVhIysDl iz;ksx dk ykslkbZ bu fd;kA xBu dk lewgksa fofHkUu dss uLyksa 3 vkSj x;k ik;k fu;r ls

93 ANNUAL REPORT 2014-15 dk;Zdkjh lkjka'k 94 ANNUAL REPORT 2014-15 dk;Zdkjh lkjka'k hl yfd Sul HUr d v;u & v/;;u ds fHkUurk iSVuZl ,yhfyd thal yík[kh eosf'k;ksa ds 72 i'kqvksa esa egRoiw.kZ dSafMMsV 6]7]8 vkSj9FkhA Øe'k% esa ,yhy Mh vkSj ,]ch]lh la[;k dh fQaxj x;sA ihvkjMh,e 9 thu ds bl Mksesu {ks= esa ftad fd;s ntZ ch) vkSj ,lh lhMh] chlh] ,ch] MhMh] lhlh] chch] (,,] thuksVkbi ukS vkSj Mh) vkSj lh] (,]ch] ,yhYl pkj fy, ds thu 9 ihvkjMh,e gSA x;k fd;k vè;;u dk ifjorZu esa thu 9 Mh-,e- ih-vkj- fy, ds {kerk iztuu mi esa i'kqvksa eos'kh vfèkd FkkA dkQh esa fnu) 30 ls (10 nqXèkdky izkjafHkd esa vkSj vfUre dky ls nqXèkdky dh vofèk dh rqyuk eè; ds Lrj ds vfHkO;fDr dh thih,,e vkSj 3 nqXèkdky pj.k esa deh vfUre ikbZ xbZA èkhj&èkhjs ,llhMh] ,Q,chih vkSj gS vfèkd dkQh nkSjku nqXèkdky ds tYnh cgqrk;r Lrj ,evkj,u, FkkA vfèkd nkSjku ds vofèk dh nqXèkdky f'k[kj vkSj dh nqXèkdky rqyuk esa lHkh dSfT+ku thuksa ds esa vfHkO;fDr iSVuZ pj.k izkjaHk vfUre vkSj eè; x;kA fd;k ewY;kadu dk thih,,e) ,llhMh] ,lh,lh,] Κ ,p,lih 70] vkSj ,p,llh 90 thuksa dh la;qDr 60] ,p,lih egRoiw. 40] ,p,lih xSj fn[kk;kA ifjorZu kZ esa vfHkO;fDr lkFk ekSle ds ifjorZu ds esa VªkalfØIV ,p,lih esa xk;ksa lkghoky ,d dkj.k gksldrkgSA ;g dk vuqdwyrk csgrj esa rqyuk dh xk;ksa dh uLy ladj fons'kh] izfr ds ruko xehZ esa HkSalksa eqjkZg vkSj xk;ksa lkghoky FkkA T;knk lcls esa xk;ksa nksuksa ÝhT+k dj.k vkSj fÝfT+k;u gksYLVhu tcfd gS] de esa HkSalksa eqjkZg dk vkSj xk;ksa lkghoky ,u@,y vuqikr x;kA fd;k fy, ds djus vkdyu dk ruko 'kkjhfjd esa xksoa'k ewY;kadu vuqikfrd rqyukRed dk (,u@,y) U;wVªksfQy@fyEQkslkbV yík[kh i'kqvksaesans[kkx;kA Hkh osfj,aV uo ij yksdl 3 & chVh,u vkSj gS x;k crk;k ugha esa uLyksa i'kq ns'kh Hkkjrh; vU; fdlh fd tks x;k ns[kk ij lh,u & ds dks bZ & oSfj,UV ns[kh gSA j[kk cuk, dks fo'ks"krkvksa vfèkd ls vfèkd xbZ iwoZ blls us eosf'k;ksa yík[kh fd gS pyrk ihvkj,y] chVh,u & 1&3 vkSj MhxsV & 1ls irk -lh,u] jk-i-vk-la-C;wjks β -lh,u] ySDV] chth,p] fiV&1] chth,p] α-ySDV] NBAGR kju wk dk ;A reu s 2 vuqlaèkku 21 esa orZeku x;kA fd;k iwjk nkSjku dqy xbZA dh tkap fy, ds ekinaMksa lkbVkstsusfVd dh lwvjksa 40 rFkk lkaMksa cSy vkSj HkSal iztuu 113 nkSjku ds o"kZ xbZA dh iznku lsok ijke'kZ fy, ds LØhfuax lkbVkstsusfVd ds lkaMks iztud fy, ds ,tsafl;ksa ls eqDr j[kus ds mís'; ds lkFk ns'k Hkj esa fofHkUu nks"kksa vkuqoaf'kd vkSj fy, ds djus tkap dh nks"kksa xq.klw= C;wjks vuqlkj ds uhfr dh ljdkj Hkkjr x;k gSA fd;k ls ekè;e ds dsanz usVodZ lEcafèkr dk;Z] dk cjxqj] vksaxksy xk; vkSj gfjau?kVk lkFk eqfxZ;ksa ds laj{k.k ds dk;Z fuèkkZj.k y{.k dk cr[k dh vle lkr iztkfr;ksaoh;ZlaxzfgrgSA vc esa cSad thu jk"Vªh; ds C;wjks gSA x;k tksM+k esa xbZ vkSj bls fiNys o"kZ ds nkSjku thu cSad esa HkaMkj dh ,df=r [kqjkd oh;Z gq, tes 7]600 dqy ds (VksMk) HkSal vkSj Fkkjikjdj) vkSj (xvksykvks xksoa'k fy, ekudhd`rfd;kx;kA ds [kqjkd oh;Z mi;ksx BUMs gq, dk tes vkSj oh;Z ?kksy vfèko`"k.kh iz'khfrr ,d ;qDr ls nokvksa ,aVhck;ksfVd vkSj tnhZ vaMk 'kdZjk cQj] lkbVªsV fu;a=; thuik,x,A vkjih,l 9 vkSj ,p,ech,l lcls fLFkj vkarfjd 9] vkjih,l th,ihMh,p] tcfd vkSj x;k ik;k mRre izn'kZu dk 15 vkjih,l }kjk dh dhij csLV vkSj ukWeZQkbUMj thukWeZ] fofèk;ksa ,YxksfjFke ,lhVhch 4 vkjih,y > 15 vkjih,l 9 = vkjih,l esa% Øe vojksgh dks thal ls viotZu inØe vkSj ij vkèkkj ds fLFkjrk dh ,Dlizs'ku thu rqyukRed x;kA fd;k ijh{k.k thu dk lanHkZ fofHkUu tkus&ekus 10 nkSjku ls Jsf.k;ksa ds dk;kZRed vè;;u ds i'kqvksa Hkkjrh; vuqdwfyr esa {ks=ksa m".kdfVcaèkh; vkSj ioZrh; mPp fn[kk;kA izHkkoh vkSj vfèkdre esa xk;ksa fÝfT+k;u gksYLVhu esa rqyuk dh xk;ksa lkghoky lkFk ,d dks vfHkO;fDr 11 vuqlaèkku ifj;kstukvksa dks fiNys o"kZ ds o"kZ fiNys dks ifj;kstukvksa vuqlaèkku 11 ,p,ech,l > phkV % ;fFr dk x;kA fd;k O;ofLFkr % ,pihvkjVh > bb,Q 1 ,1 1 bb,Q > hiM, th,ihMh,p > fgUnh [k.M ;q,DlVh > ch2,e > > e.ij efZka h ok"r^ j d fnolh; ,d dk;Z'kkyk dhxbZA ij fo'ks"krk^ dh dks eqfxZ;ksa 21-11-2014 *ef.kiqjh esa bEQky lg;ksx ds'keyksax] ds ls bEQky fo'ofo|ky; Ïf"k dsUæh; dk;ZØe vk;ksftrfd;kx;kA izf'k{k.k fnolh; rhu ,d ls 'kh"kZd rdÞ iz;ksx ls rduhd fo'ys"k.k% vuqØe.k dk ih<+h ßvxyh 16 tqykbZ]2014dksC;wjksesaeuk;kx;kA fnol LFkkiuk dk ifj"kn~ vuqlaèkku Ïf"k Hkkjrh; fy;kA Hkkx esa lEesyuksa vkSj laxksf"B;ksa dk;Z'kkykvksa] vkSj izf'k{k.k Hkhrj ds ns'k us oSKkfudksa fofHkUu ds C;wjks leh{kk dhxbZA dh izxfr eè; vofèk vkbZvkjlh dh cSBd ds nkSjku le; ij vk;ksftr dh xbZA vuqlaèkku ifj;kstukvksa cSBds dh (vkbZvkjlh) lfefr vuqlaèkku laLFkku izn'kZfu;k¡ yxkbZxbZA ds fy, fofHkUu LFkkuksa ij i'kq vuqoaf'kd laink ij crkus ukS dks fdlkuksa esa ckjs ds ykHkksa ds i'kqvksa Lons'kh vkSj djus izn'kZu dk dk;ksZa ds xfrfofèk;ksa ij : 10]21]195@& [kpZ fd;s x,A laLFkku dh if=dkvksa vkSj iqLrdksa esa iqLrdky; yhA lnL;rk dh Hkkjrh;) 31 vkSj 11 (fons'kh if=dkvksa 44 dh fodkl oSKkfud@rduhdh us iqLrdky; C;wjks lexz Ldksj gkfly djds mRd`"V jsfVax izkIr dh gSA vkj,QMh ds rgr C;wjks us 99-50 izfr'kr dk ,d fnYyh dksizLrqrfd;kx;kA ubZ dk;kZy;] isVsaV Hkkjrh; vuqjksèk ijh{kk igyk esa izdkf'kr fd, x, vkSj rhu isVsaV vkosnuksa ds fy, tuZy ds dk;kZy; isVsaV Hkkjrh; vkosnu isVsaV lkr izdkf'kr fd,x,A }kjk laLFkku Hkh eSuqvy izf'k{k.k vkSj eksuksxzkQ 12 x,A fd, izdkf'kr ys[k rduhdh@yksdfiz; vkSj jk"Vªh; 27 x,A fd, izHkkoh izdkf'kr esa if=dkvksa mPp varjjk"Vªh; i= 'kksèk 43 dqy jk"Vªh; QSyksifj;kstuktkjhgSA ,d vkSj iksf"kr foRr ckg; ,d vkSj ifj;kstuk, fgUnh [k.M NBAGR

fuoZ`r gq,A ,d izèkku oSKkfud ,oa ,d rduhdh vfèkdkjh lsok nkSjku ds o"kZ gqvkA ij in ds (m-iz-) esjB laLFkku] vuqlaèkku xksoa'k dsUnzh; & Hkk-Ï-vuq-i- funs'kd] C;wjks ds ,d izèkku oSKkfud Mk- ch izdk'k dk p;u] lgk;d deZpkfj;ksadhle;kuqlkjinksUufrgqbZA dq'ky 3 ,oa vfèkdkfj;ksa rduhdh 2 oSKkfudksa] 6 ds laLFkku nkSjku ds o"kZ gSaA dk;Zjr deZpkjh lgk;d& dq'ky 5 ,oa iz'kklfud 19 vfèkdkjh] rduhdh 17 oSKkfud 29 dqy esa C;wjks esa orZeku tk jgkgSA vè;kiu o 'kksèk dk;ksZa esa ekxZfunsZ'ku gsrq dk;Z fd;k esa fo|kfFkZ;ksa ds okpLifr foèkk ,oa LukRdksÙkj }kjk ds cjsyh lnL;ksa ladk; gSaA 'kkfey esa :i ds lnL; ladk; bTtruxj] vkbZ-oh-vkj-vkbZ-] djuky ,oa ,u-Mh-vkj-vkbZ-] oSKkfud] ds laLFkku esa vUrjkZ"Vªh;izfrHkkfx;ksadksizf'k{k.kiznkufd;kA :i esa] vkbZ , bZ , }kjk izk;ksftr izf'k{k.k dk;ZØe ds izoDrk fo'ks"kK mUgksaus ij tgka fd;k] nkSjk dk funs'kd] jk-i-vk-la- C;wjks djuky us vkfLVª;k fo,uk Mk- ftehfLeFkizeq[kjgsA egkfuns'kd ds vkbZ-,y-vkj-vkbZ- ftlesa fd;k] o"kZ ds nkSjku dbZ fof'k"V O;fDr;ksa us C;wjks dk nkSjk 21 izfrHkkfx;ksausfgLlkfy;kA dqy ftlesa x;k] fd;k vk;ksftr dk;ZØe izf'k{k.k ubZ vofèk y?kq dk fnuksa 10 ,d izk;ksftr }kjk fnYyh Hkk-Ï-vuq-i- ij 'kh"kZd fjlkWlstß tsusfVd QkWj thukse dSjsEVjkbts'ku vkWQ baMhftul ,fuey ß,MokaLM ekWyhD;wyj ,.M ck;ksbUQkesZfVd vizkspsl ds lg;ksxlsvk;ksftrdhx;hA cksMZ fodkl i'kqèku flfDde esa] xaxVksd dk;Z'kkyk LVkWfeZxa czsu lalkèku ,d dks 19-11-2014 vkuqoaf'kd fnukad ij i'kqèku ds jkT; flfDde vk;ksftr dhxbZA ls #i la;qDr }kjk C;wjks vkSj ljdkj Hkkjr ,Q] vkSj Mh,,pMh dks 28&01&2015 dk;Z'kkyk baVjSfDVo ,d ij izfØ;k^ iathdj.k *uLy esa C;wjks jk-i-vk-la-C;wjks

95 ANNUAL REPORT 2014-15 dk;Zdkjh lkjka'k 96 ANNUAL REPORT 2014-15 jktHkk"kk izdks"B dh xfrfof/k;k¡ x;kA funs'kd }kjk xfBr dk;ZØe vk;kstu lfefr vk;kstu dk;ZØe xfBr }kjk funs'kd x;kA fd;k vk;kstu dk dk;ZØeksa fgUnh fofHkUu varxZr ds i[kokM+k psruk fgUnh gsrq c

sks ds qL` f; xk b if;sxk esa izfr;ksfxrk bl x;kA fd;k iqjLd`r dks ys[kksa vad (o"kZ 2013) esa Nis 'kksèk ys[kksa esa ls rhu Js"B prqFkZ ds i'kqèku if=dk fganh okf"kZd dh laLFkku gh nkSjku ds lekjksg forj.k iqjLdkj jktHkk"kk okf"kZd fd;sA forfjr iqjLdkj dks fotsrkvksa gq, djrs ljkguk dh dk;ZØeksa fganh bu x, fd;s vk;ksftr esa Hkk"k.k Hkkjrh; Ïf"k oSKkfud p;u eaMy us vius vè;{kh; lnL;] ekuuh; ca|ksikè;k;] ds- ,l- MkW- vfrfFk volj ij iqjLÏr fd;k x;kA bl volj ij eq[; ds lekjksg fnol LFkkiuk ds C;wjks dks 09-2014 ekSf[kd izfr;ksfxrkvksa ds fotsrkvksa dks fnukad 20- o fyf[kr gqbZ nkSjku ds flrEcj 12&19 fnukad dqekj usthrkA vouh'k MkW- iqjLdkj f}rh; rFkk flag d.kZohj MkW- iqjLdkj izFke esa okyksa cksyus esa foi{k thrkA us lks<+h eksfudk MkW- iqjLdkj f}rh; 'kekZ] iqjLdkj js[kk MkW- izFke gsrq cksyus esa i{k ds izfr;ksfxrk bl x;kA fn;k le; dk feuV 5 dks izfr;ksfxvksa gsrq cksyus esa foi{k o i{k ds izHkko fo"k; FkkA x;k j[kk ij laLÏfr Hkkjrh; dk lH;rk if'peh fo"k; ftldk x;k fd;k vk;kstu dk izfr;ksfxrk okn&fookn ,d gh dks 18-09-2014 fnukad r`rh; rFkk iqjLdkj JherhvuhrkpankusthrkA lks<+h eksfudk MkW- iqjLdkj f}rh; flag] d.kZohj MkW- iqjLdkj izFke vkSj jgs izfrHkkxh 17 dqy esa izfr;ksfxrk bl fd;kA ls ekè;e ds jk-i-vk-la-C;wjks

97 ANNUAL REPORT 2014-15 jktHkk"kk izdks"B dh xfrfof/k;k¡ 98 ANNUAL REPORT 2014-15 jktHkk"kk izdks"B dh xfrfof/k;k¡ iathdj.k & jk"Vªh; laink dh lqj{kkß dks feykA dks lqj{kkß dh laink jk"Vªh; uLy & Þi'kqèku iathdj.k i= 'kksèk ds djuky] ,-th-vkj- ,u-ch- tks'kh] ch-ds- MkW- o VkafV;k ,e-,l- MkW- fujatu] ,l-ds- MkW- fot] ih-ds- MkW- iqjLdkj izFke jk-i-vk-la-C;wjks NBAGR

}kjk fd;kx;kA Ï-oS-p-e- lnL; ca|ksikè;k;] ds- ,l- MkW- vfrfFk eq[; ekuuh; foekspu dk 2014) (o"kZ vad iape ds izdk'k i'kqèku if=dk gh fgUnh okf"kZd dh esa laLFkku lekjksg forj.k iqjLdkj jktHkk"kk okf"kZd mfpr j[k&j[kkoßusthrkA dk Þi'kqvksa i= 'kksèk ds iaruxj fo-fo-] izks- o d`f"k xks-c-ia- egkfoè;ky;] foKkua i'kq o fpfdRlk i'kq flag] ozr nso MkW- iqjLdkj r`rh; feykA peqFkhZ dks ?kksM+kß & èkjksgj vewY; dh izns'k Þfgekpy i= 'kksèk ds ikyeiqj fg-iz-d`-fo-fo- dkWyst] fpfdRlk ;'kiky Bkdqj] o#.k la[;ku ,oa jkds'k dqekj] i'kq f}rh; LFkku MkW- iznhi dqekj Mksxjk] lathr dVksp] fgUnh [k.M