Evolutionary History of Cuban Crocodiles Crocodylus Rhombifer 315:358-375,2011 and Crocodylus Acutus Inferred from Multilocus Markers
RESEARCH ARTICLE Evolutionary History of Cuban J OURNAL OF Crocodiles Crocodylus rhombifer E XPERIMENTAL and Crocodylus acutus Inferred Z OOLOGY ECOLOGICAL GENETICS AND PHYSIOLOGY From Multilocus Markers Ajournal oflntegrativc Biology YOAMEL MILIAN-GARCiA1, MIRYAM VENEGAS-ANAYA2,3, ROBERTO FRIAS-SOLER1, ANDREW J. CRAWFORD2'4, ROBERTO RAM0S-TARGAR0NA5, ROBERTO R0DRIGUEZ-S0BER0N5, MANUEL AL0NS0-TABET5, JOHN THORBJARNARSON61", ORIS I. SANJUR2, 1 2 GEORGINA ESPINOSA-LOPEZ *, AND ELDREDGE BERMINGHAM 1 Facultad de Biologia, Universidad de La Habana, Ciudad Habana, Cuba ^Smithsonian Tropical Research Institute, Panama, Republic of Panama ^Department of Biological Sciences, Texas Tech University, Lubbock, Texas 4Departamento de Ciencias Biologicas, Universidad de los Andes, Bogota, Colombia 5Empresa Nacional para la Proteccion de la Flora y la Fauna, Miramar, Ciudad La Habana, Cuba 6Wildlife Conservation Society, Gainesville, Florida ABSTRACT Among crocodilians, Crocodylus rhombifer is one of the world's most endangered species with the smallest natural distribution. In Cuba, this endemic species coexists with the American crocodile (Crocodylus acutus). Hybridization between these two species is well known in captivity and might occur in the wild, but has never been demonstrated genetically. Here, we combined molecular data with environmental, geographic, and fossil data to infer the evolutionary history of Crocodylus in the Cuban Archipelago, and to evaluate genealogical support for species boundaries. We analyzed seven microsatellite loci plus DNA sequence data from nuclear (RAG-1) and mitochondria! (cytochrome b and cytochrome oxidase I) genes from 89 wild-caught individuals in Cuba, Grand Cayman Island, Jamaica, and Central America, and two samples from zoo collections. Microsatellites showed evidence of introgression, suggesting potential hybridization among Cuban groups.
[Show full text]