SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the US03CBCH21 Biochemistry of Bio Molecules I Course Total Credits Hours per 4 4 of the Course Week
Course Student should be able to: Objectives: 1. Importance of bio molecules in living cells 2. Chemistry of bio molecules …
Course Content
Unit Description Weightage* (%)
1. Carbohydrates and glycobiology. 25 Definition, classification and functions of carbohydrates. Occurrence and biochemical roles of monosaccharide. Disaccharides (maltose, sucrose and lactose). Oligosaccharides (Raffinose). Polysaccharides (starch, glycogen, cellulose, heparin). Physical properties – Asymmetric carbon, D and L isomers, isomerism, mutarotation, optical activity, epimers, cyclization. Chemical properties – Osazone formation, action of acids and alkali on sugars Derivatives of Sugars with example.
2. Nucleotide and Nucleic acids: 25 1. Functions and composition of nucleic acids 2. Structure of nucleotides, purines and pyrimidines. 3. Nomenclature of nucleotides. 4. DNA (a) Structure of DNA. (b) DNA double helix (Watson and crick model). (c) Chargaff’s rule of DNA compositions. (d) Different forms of DNA (Double Helix). (e) The size of DNA. (f) Organization of DNA in the cell. (g) Denaturation & Renaturation of DNA strands. 3. Vitamins 25 Definition & Classification of Water soluble and fat soluble vitamins. Sources, chemical nature (without structure) functions of vitamins. Roles of Coenzymes
4. Minerals 25 Definition& classification Macro and Micro minerals - their sources, RDA.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Role of Minerals as Co-factor ( biochemical reaction) General Functions of Minerals. Disorders of Macro Minerals.
Teaching- Topics will be taught and discussed in interactive sessions using Learning conventional black board and chalk as well as ICT tools such as power Methodology point presentations and videos. Practical sessions will be conducted in a suitably equipped laboratory either individually or in groups depending on the nature of exercise as well as availability of infrastructure. Course materials will be provided from primary and secondary sources of information.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understanding of bio molecules and their involment in living cells.
Suggested References:
Sr. References No. 1. Biochemistry Mathews –Van Holde.
Fundamentals of biochemistry Dr. J.L Jain, Dr.Sanjay Jain. Nitin Jain.
Textbook of medical biochemistry :7th Edition – Rana Shinde, M.N.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Chatterjea
Outlines of Biochemistry Eric E. Conn, Paul k. Stumpf, George Bruening Roy H. Doi
Biochemistry U.Satayanarayana, U Chakrapani.
Textbook of Biochemistry for medical students RAFI MD.
. Zubay’s Principles of biochemistry VeerBala Rastogi K.R. Aneja.
Textbook of biochemistry for medical students DM. Vasudevan, Sreekumari S. Kannan Vaidyanathan.
On-line resources to be used if available as reference material
On-line Resources
Related References Books
*****
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the BIOPHYSICAL US03CBCH22 Course BIOCHEMISTRY Total Credits Hours per 4 4 of the Course Week
Course 1. Should have knowledge about universal solvent, water. Objectives: 2.Acid base balance 3. should know radio isotopes …
Course Content
Unit Description Weightage* (%)
1. UNIT-1 WATER:- 25 1) Structure and solvent properties. 2) Colligative properties of water. 3) Distribution of body water. 4) Normal water balance. 5) Regulatory mechanism of normal water balance. A) Role of kidney. B)Thirst mechanism. C) Role of ADH and Aldosterone 6) Abnormal water balance. A) Pure water Dehydration. B) Pure Salt Dehydration. C) Mixed type (water & Salt) Dehydration D) Over hydration.
2. Acid, Base, and Buffer:- 25 1.Definition of Acid, Base, Buffer & pH and pH measurement methods. 2. Physiological Buffer System. A. Intracellular B. Extracellular 3. Titration curve and pka of weak acid 4. H-H equation & its example to find PH and pka
3. Biophysical Biochemistry:- 25 1) Colloid:-Definition & classification of colloids 2) Properties of colloids (in short). (A) Tyndal effect (B) Dialysis (C) Brownian movement (D) Ageing (E) Coagulation (F) Electrical
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Properties. 3) Biological significance of Colloids. 4) Definition & significance of - Viscosity, surface tension, Osmosis, Diffusion, Donnan membrane equilibrium.
4. Radioisotopes 25 1) Introduction of isotopes:-atomic Numbers, mass numbers, isotopes Radioisotopes, unit of radio activity, Half lives of isotopes. Radio acive decay. 2) Techniques used in mesurement of radioactivity:- Principle and method of Autoradiography, Scintillation Counting, Geigermuller Counter. 3) Biological hazards of radiation and its safety. 4) Significances and applications of radio isotopes in various field of Biological science and medical field.
...
Teaching- Topics will be taught and discussed in interactive sessions using Learning conventional black board and chalk as well as ICT tools such as power Methodology point presentations and videos. Practical sessions will be conducted in a suitably equipped laboratory either individually or in groups depending on the nature of exercise as well as availability of infrastructure. Course materials will be provided from primary and secondary sources of information.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understanding of biological buffer and importance of radio isotops.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Suggested References:
Sr. References No.
1. Biophysical Chemistry BY Upadhayay and Nath.
2 Principles of biochemistry – lehninger.
3 Human Physiology By C.C.Chetargy.
4 Hawks biochemistry.
On-line resources to be used if available as reference material
On-line Resources
Related References Books
*****
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the US03CBCH23 BIOCHEMISTRY PRACTICAL Course Total Credits Hours per 8 4 of the Course Week
Course 1. Ability to apply the fundamental knowledge of carbohydrates. Objectives: 2. Ability to prepare the chemical solutions which are prepare for the practicals. 3. Ability to how to calculate the practical calculation.
Course Content
Unit Description Weightage* (%) 1. (BIOCHEMISTRY PRACTICAL) 100 • Rules and regulations in a biochemistry • laboratory.Prepration of Normal ,% solution( understanding of practical calculation). • Estimation of Vitamin – C by titrametric method. • Qualitative analysis of carbohydrate. • Quantitative analysis by Beer’s Law / lamda max.( KmnO4) • Estimation of Iron. • Estimation of Phosphorous.
Teaching- Practical sessions will be conducted in a suitably equipped laboratory Learning either individually or in groups depending on the nature of exercise as well Methodology as availability of infrastructure. The module will consist of seminars, in which the students will be supported in their practical teaching classes This practical session will also involve them observing their peers and participating in observation tasks and oral feedback. There will be a focus on particular experiment perform by students.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understanding of bio chemical analysis and estimation of various bio molecules
2. Demonstrate a practical understanding of theories and concepts
3. Gain practical as well as theoretical knowledge of Biomolecues, vitamins and minerals.
Suggested References:
Sr. References No.
1. Standard methods of biochemical analysis – s.r. thimmaiah.
2 Practical cilinical biochemistry methods & interepretations – ranjana chawla.
3 Practical biochemistry by harold varley.
4 Laboratry manual in biochemistry – by j.jayaraman
5 Lab manual in biochemistry, immunology & biotech – by Arti Nigam, Archana Ayyagiri
6 Laboratry manual in biochemistry – By Subrayaman
On-line resources to be used if available as reference material
On-line Resources
Related References Practical Books
Page 2 of 3
SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
*****
Page 3 of 3
SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the Biochemistry of Biomolecules II US04CBCH21 Course Total Credits Hours per 4 4 of the Course Week
Course 1.Shoud have knowledge about proteins, lipids, minerals Objectives:
Course Content
Unit Description Weightage* (%)
1. Amino acids 25 _ Proteins are polymers of amino acids, standard amino acids _ Classification of amino acids – Based on structure. Aminoacids with aliphatic side chains. Hydroxyl group containing aminoacids. Sulphur containing amino acids. Acidic amino acids and theirs amids. Basic amino acids. Aromatic amino acids and Imino acids. Nutritional classification of amino acids. Classification based on metabolic fate. Classification based on their solubility. Non standard or uncommon or rare amino acids – Non protein amino acids Physical Properties – Isomerism, Isoelectric pH, Zwitter ion, Isoelectric point (IEP), Sorenson’s titration curve. _ Chemical properties – Reactions involving (-COOH) group, Reaction Involving (-NH2) group, Reactions involving side chains.
2. Proteins 25 Function and classifications. An overview of protein structure, peptide bond, primary,secondary,tertiary and quaternary structure. _ Forces stabilizing the tertiary structure – Hydrophobic interaction, hydrogen bonds, ionic interactions, Van der Wals interactions and covalent cross linkages. Biological Important peptides. Protein denaturation. Precipitations reactions of proteins.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
3. Chemistry of Lipids 25 _ Definition, classification and functions of Lipids _ Simple, compound, derived and miscellaneous lipids. _ Fatty acids – free and esterified fatty acids, length of hydrocarbon chain, straight and branched chain fatty acids, hydroxyl and cyclic fatty acids, saturated and unsaturated fatty acids , nomenclature of fatty acids, omega classification of fatty acids, essential fatty acids, dietary sources and functions of fatty acids. _ Introduction to: Triacylglycerols, phospholipids, sphingophospholipids, glycolipids, lipoproteins, cholesterol.
4. A) Enzymes 25 Definitions Classification with examples General Functions (B) Hormone (Human) Definition Classification with examples General Functions Brief account on Hormonal imbalance
...
Teaching- Topics will be taught and discussed in interactive sessions using Learning conventional black board and chalk as well as ICT tools such as power Methodology point presentations and videos. Practical sessions will be conducted in a suitably equipped laboratory either individually or in groups depending on the nature of exercise as well as availability of infrastructure. Course materials will be provided from primary and secondary sources of information.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Course Outcomes: Having completed this course, the learner will be able to
1. Understanding of chemistry, classification, functions and food sources of proteins, lipids and minerals
Suggested References:
Sr. References No.
1. 1. Biochemistry Mathews –Van Holde. 2. Fundamentals of biochemistry Dr. J.L Jain, Dr.Sanjay Jain. Nitin Jain. 3. Texbook of biochemistry for medical students DM. Vasudevan, Sreekumari S. Kannan Vaidyanathan. 4. Zubay’s Principles of biochemistry VeerBala Rastogi K.R. Aneja. 5. Textbook of Biochemistry for medical students RAFI MD. 6. Biochemistry U.Satayanarayana, U Chakrapani. 7 Outlines of Biochemistry Eric E. Conn, Paul k. Stumpf, George Bruening , Roy H. Doi 8. Textbook of medical biochemistry Dinesh Puri. 9. Textbook of medical biochemistry: 7th Edition – Rana Shinde, M.N. Chatterjea
On-line resources to be used if available as reference material
On-line Resources
Related References Books
*****
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the CELL BIOLOGY US04CBCH22 Course Total Credits Hours per 4 4 of the Course Week
Course 1. Should know about structure and function of living cells. Objectives:
Course Content
Unit Description Weightage* (%)
1. CELL STRUCTURE AND FUNCTION:- 25 Structural Organization of Plant Cell and Animal Cell, Difference between Plant Cell and Animal Cell. Origin and Evolution of life from a single cell. General features and Biochemical Composition for following Organelles: -Plasma membrane / Cell wall, Mitochondria, Golgi complex, Ribosomes, Lysosomes, Peroxisome, Chloroplast, ER (Endoplasmic Reticulum), Cytoplasm.
2. Cytoskeleton:- 25 Actin filament: -Structure and Function, Polymerization and De Polymerization. Microtubule:-Symmetric -Assembly and Dynamic instability and microtubule. Centrosome: - location and Function, chromosome segregation. Tread milling, intermediate filament Polymerization mechanism. Centrioles: - Structure and Function
3. Cell Cycle:- 25 Cell Cycle- Phases of cell cycle, Molecular events during cell cycle phases. Cell Cycle Regulation, cyclin Dependent kinases, Mitosis and meiosis.
4. Apoptosis and Stem cell:- 25 Types of Apoptosis, Mechanism of apoptosis, Stem cell types,
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Significance and Application.
...
Teaching- Topics will be taught and discussed in interactive sessions using Learning conventional black board and chalk as well as ICT tools such as power Methodology point presentations and videos. Practical sessions will be conducted in a suitably equipped laboratory either individually or in groups depending on the nature of exercise as well as availability of infrastructure. Course materials will be provided from primary and secondary sources of information.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understanding of structural and functional unit of life.
2. Knowledge of various cellular organelles.
Suggested References:
Sr. References No.
1. REFRENCE Books:- 5. The Cell- A molecular Approch Geoffrey and cooper. 6. Cell Biology – C.b. Pawar Himalaya Publication. 7. Cytology – varma and Agrawal. 8. Cell and Molecular Biology –De Roberti’s -8th edition. 9. Cell and Molecular Biology John wiley and sons. 10. Mol.biology and the cell-Albert.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
11. Plant Physiology by Salisbury & Ross. 12. Plant Biochemistry by Hans-walter Heldt.
On-line resources to be used if available as reference material
On-line Resources
Related References Books
*****
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the BIOCHEMISTRY US04CBCH23 Course PRACTICAL Total Credits Hours per 8 4 of the Course Week
Course 1. Ability to apply the fundamental knowledge of Biomolecues, protein, Objectives: biochemical techniques in the area of biochemistry. 2. An ability to learn a system of cell components. 3. The principle components and uses of a light microscope. 4. Different staining procedures are used for the stages of mitosis.
Course Content
Unit Description Weightage* (%) 1. (BIOCHEMISTRY PRACTICAL) 100 • Study of Protein (amino acids) colour reaction. Study of protein precipitation. • Estimation of protein by Biuret method. Quantitative analysis of Lipid. • Identification of Amino Acid by Paper Chromatography. • Estimation of fatty acid by titrametic analysis. Study of salivary amalylase. • Study of compound microscope. Vital staining by Evan’s Blue. • Demonstration of mitochondria by staining. Study of different stages of mitosis. • Study of nucleus. • Study of plant cell
Teaching- Practical sessions will be conducted in a suitably equipped laboratory Learning either individually or in groups depending on the nature of exercise as well Methodology as availability of infrastructure. The module will consist of seminars, in which the students will be supported in their practical teaching classes This practical session will also involve them observing their peers and participating in observation tasks and oral feedback. There will be a focus on particular experiment perform by students.
Evaluation Pattern
Page 1 of 3
SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understanding of bio chemical analysis and estimation of various bio molecules
2. Understand the staining procedures which are used for the stages of mitosis.
3. Gain the knowledge of chromatography.
Suggested References:
Sr. References No.
1. Standard methods of biochemical analysis – s.r. thimmaiah.
2 Practical cilinical biochemistry methods & interepretations – ranjana chawla.
3 Practical biochemistry by harold varley.
4 Laboratry manual in biochemistry – by j.jayaraman
5 Lab manual in biochemistry, immunology & biotech – by Arti Nigam, Archana Ayyagiri
6 Laboratry manual in biochemistry – By Subrayaman
On-line resources to be used if available as reference material
Page 2 of 3
SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
On-line Resources
Related References Practical Books
*****
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the Course US05CBCH21 MOLECULAR BIOLOGY-I Total Credits Hours per 3 of the Course Week
Course 1.The knowledge of cell structure and function ,knowledge of how Objectives: eukaryotic cells work at the molecular level. 2. Provide an overview of cell structure and function at the molecular level, including the flow of information from genes to proteins, and regulation of cellular processes, signalling and proliferation in eukaryotic cells. 3. Introduce some of the major ideas and experimental approaches in cell and molecular biology. 4. Introduce the student into the world of Molecular Biology, to understand the transfer of genetic information from nucleic acid till protein synthesis and cell function.
Course Content
Unit Description Weightage* (%)
1. Basic concept of molecular biology 25% 1. Definition and concept of gene 2. Concept of chromosome, Chromatin and genome 3. Histones, Nucleosomes and packaging of DNA into chromosomes 4. Salient features of viral genome, prokaryotic and eukaryotic genome
2. 25% DNA Replication 1. Introduction and definition of replication , Replisome and primosome
2. Set of fundamental rules for DNA replication a) DNA replication is semiconservative- Meselson and Stahl experiment b) DNA synthesis proceeds in 5’ to 3’ direction and is semi discontinuous
3. Prokaryotic DNA polymerases: Activities of DNA Polymerase I, II and III. 4. Mechanism of replication: Initiation, Elongation and Termination.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
6. Differences in prokaryotic and eukaryotic replications
3. 25% Transcription 1. RNA polymerases in prokaryotic and eukaryotic organisms 2. Promoters in prokaryotic and eukaryotic organism 3. Mechanism of transcription: Initiation, Elongation and Termination. 4. Post translation modification
a) Splicing mechanism of group 1,2,3,4 b) Generation of 5’ cap in m RNA c) 3’ poly a tail formation 5. Reverse transcriptase and Reverse transcription
4. Translation / protein synthesis 25% 1. Genetic code: Definition and properties
2. Role of ribosomes, m RNA and t RNA in transcription a)formation of fmet tRNA b) Activation of amino acid c) Initiation d) Elongation e) Termination f) Post translation modification 3. Inhibition of protein synthesis by antibiotics and toxins.
...
Teaching- Theoretical lectures, ppt presentations, seminars and practical. Learning Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. The scientific process, in the context of learning the fundamental biological and chemical ‘facts' of molecular biology.
2. Gain skills required to effectively do scientific research.
3. Understand and apply general concepts of cell and molecular biology to relevant, specific problems.
4. Link the rapid advances in cell and molecular biology to a better understanding of diseases, including cancer.
Suggested References:
Sr. References No.
1. Watson, J. D., Baker T.A., Bell, S. P., Gann, A., Levine, M., and Losick, R., 2008 Molecular Biology of the Gene 6th edition. Cold Spring Harbour Lab. Press,Pearson Pub.
2. Freifelder’s Essentials of Molecular Biology by George M. Malacinski
3. Nelson and Cox, Lehninger’s Principles of Biochemistry (2000), Worth Publish., Inc. NewYork.
On-line resources to be used if available as reference material
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
On-line Resources
*****
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the Course US05CBCH21 MOLECULAR BIOLOGY-I Total Credits Hours per 3 of the Course Week
Course 1. Objectives: 2. …
Course Content
Unit Description Weightage* (%)
1. Basic concept of molecular biology 25% 1. Definition and concept of gene 2. Concept of chromosome, Chromatin and genome 3. Histones, Nucleosomes and packaging of DNA into chromosomes 4. Salient features of viral genome, prokaryotic and eukaryotic genome
2. 25% DNA Replication 1. Introduction and definition of replication , Replisome and primosome
2. Set of fundamental rules for DNA replication a) DNA replication is semiconservative- Meselson and Stahl experiment b) DNA synthesis proceeds in 5’ to 3’ direction and is semi discontinuous
3. Prokaryotic DNA polymerases: Activities of DNA Polymerase
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
I, II and III. 4. Mechanism of replication: Initiation, Elongation and Termination. 6. Differences in prokaryotic and eukaryotic replications
3. 25% Transcription 1. RNA polymerases in prokaryotic and eukaryotic organisms 2. Promoters in prokaryotic and eukaryotic organism 3. Mechanism of transcription: Initiation, Elongation and Termination. 4. Post translation modification
a) Splicing mechanism of group 1,2,3,4 b) Generation of 5’ cap in m RNA c) 3’ poly a tail formation 5. Reverse transcriptase and Reverse transcription
4. Translation / protein synthesis 25% 1. Genetic code: Definition and properties
2. Role of ribosomes, m RNA and t RNA in transcription a)formation of fmet tRNA b) Activation of amino acid c) Initiation d) Elongation e) Termination f) Post translation modification 3. Inhibition of protein synthesis by antibiotics and toxins.
...
Teaching- Learning Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1.
2.
3.
…
Suggested References:
Sr. References No.
1. Watson, J. D., Baker T.A., Bell, S. P., Gann, A., Levine, M., and Losick, R., 2008 Molecular Biology of the Gene 6th edition. Cold Spring Harbour Lab. Press,Pearson Pub.
2. Freifelder’s Essentials of Molecular Biology by George M. Malacinski
3. Nelson and Cox, Lehninger’s Principles of Biochemistry (2000), Worth Publish., Inc. NewYork.
On-line resources to be used if available as reference material
On-line Resources
Page 6 of 7
SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
*****
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US05CBCH22 Title of the ENZYMOLOGY Course Total Credits Hours per 3 of the Course Week
Course 1. To teach the student majoring in science all the major aspects of the study of Objectives: enzymes. 2. The theories of enzyme kinetics, the mechanisms of enzyme catalysis, and the mechanisms of enzyme regulation in the cell. 3. Use of enzymes in biotechnology and bioanalytics including biosensors and enzyme reactors. …
Course Content
Unit Description Weightage* (%) 1. Enzymes 25% Definition, historical perspective, general characteristics of enzymes. Nomenclature and classification based on IUB with examples. Unit of enzyme activity – definition of IU, enzyme turn over number and nature of non-enzymatic and enzymatic catalysis. Coenzymes – Structure and function, metallo-enzymes and metal activated enzymes; Enzyme specificity, the concept of active site, determination of active site. Stereo specificity of enzymes, Lock and key Model, Koshland’s induced fit theory 2. 25% Isolation and purification of enzymes Isozymes and their importance, Lactate dehydrogenase as an example, allozymes, Proteolytic enzymes: endopeptidase and exopeptidase; Multienzyme complex: Pyruvate dehydrogenase and fatty acid synthase 3. Enzyme kinetics 25% Factors affecting rates of enzyme catalyzed reactions, Effect of enzyme concentration, substrate concentration, pH and temperature. uni-substrate reactions, concept of Michaelis -Menten, Determination and significance of kinetic constants, catalytic rate constant and specificity constant, Limitations of Michaelis-Menten Kinetics. Lineweaver – Burk(L-B) plot. Determination of Vmax & Km from L-B plot and their significance Classification and kinetics of multisubstrate reactions. Reversible and irreversible inhibition, competitive, non competitive and uncompetitive
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
inhibitions. 4. Regulation of enzyme activity 25% Reversible, Irreversible, allosteric and feedback inhibition, Allosteric enzymes, Sigmoidal curve, positive and negative modulators, positive and negative co-operativity examples with special reference to aspartate transcarbamylase and phosphofructokinase Abzymes, Ribozymes, Industrial and biomedical applications of enzymes. Mechanism of enzyme action Mechanism of enzyme action.
...
Teaching- This course will be taught by a mixture of discussion, lecture, readings, smart - Learning board technology, on-line quizzes and student presentation modalities. Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Student will learn about structure and function of enzymes, principles of enzyme catalysis and applications of enzymes in research and industry.
2. Use the equations of enzyme kinetics.
3. The catalytic mechanisms employed by the most well-characterized
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Suggested References:
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Sr. References No.
1. Understanding Enzymes by Trevor Palmer
2. Fundamentals of Enzymologist : Nicholes C. Price and Lewis Stevens, Oxford Univ. Press.
3. Enzymes: M. Dixon, E. C. Webb, CJR Thorne and K. F. Tipton, Longmans, London
4. L. Stryer BIOCHEMISTRY W.H. Freeman Co., San Francisco, USA
On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US05CBCH23 Title of the HUMAN METABOLISM - I Course Total Credits Hours per 3 of the Course Week
Course 1. 1. Explain major catabolic and anabolic pathways in metabolism of Objectives: carbohydrates, lipids, amino acids and nucleotides. 2. Recall the key regulatory points in metabolic pathways. 3. Explain how diet and hormonal signalling regulate metabolic pathways. 4. Recognize the role of vitamins and minerals 5. Explain how certain medicines and drugs impinge upon metabolic pathways 6. Define biochemical functions and integrated metabolism of in brain, digestive system, liver, red cell, muscle and adipocyte. 7. Explain molecular mechanisms underlying major inherited diseases of metabolism. 8. Connect specific symptoms in clinical case presentations to metabolic disorders. …
Course Content
Unit Description Weightage* (%)
1. CARBOHYDRATE METABOLISM 25% Basic concept: Anabolism, Catabolism, Metabolic pathways (liner, cyclic, metabolic sequences), Regulation of metabolic pathway, Why study metabolism , Significance in medicine, Types of food stuffs ,Breakdown of food stuff overview, Functional anatomy of digestive system Digestion and absorption of carbohydrate (In brief) The entry of glucose into cells: Sodium-Glucose symport, Uptake by glucose transporter Glycolysis: Energetics of glycolysis, Regulation of glycolysis, Inhibitors of glycolysis, Conversion of pyruvate to lactate Rapaport - Leubering Cycle Gluconeogenesis and its regulation Hexose monophosphate shunt Glucose-alanine cycle and Cori cycle Krebs cycle: Energetics of Krebs cycle, Regulation of Krebs cycle
2. CARBOHYDRATE METABOLISM AND ASSOCIATED 25% DISORDERS Hormonal regulation of Carbohydrate metabolism (role of Insulin & Glucagon) Glycogen metabolism : Role of glycogen, Glycogenesis &
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Glycogenolysis Glycogen storage diseases (Type 0, Type I – VII) Glycosuria, Pentosuria, Fructosuria Diabetes mellitus Hunter’s Syndrome or mucopolysaccharidosis
3. .LIPID METABOLISM 25% Digestion, absorption and transport of lipid in blood, Alpha, beta and omega Oxidation of Saturated Fatty acid Oxidation Unsaturated, and Odd carbon chain of fatty acids Biosynthesis of Fatty acids, TG, Cholesterol Synthesis and degradation of Ketone bodies Hormonal regulation of lipid metabolism
4. DISORDERS OF LIPID METABOLISM 25% Hypercholesterolemia Gaucher’s disease Tay Sach’s disease Refsum disease Niemann pick disease
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Teaching- This course will be taught by a mixture of discussion, lecture, readings, smart - Learning board technology, on-line quizzes and student presentation modalities. Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
1. The focus is on the regulation of sugar and fat metabolism in eukaryotes, with an emphasis on mammals. The course will begin with a review of carbohydrate and lipid metabolic pathways, particularly pathway integration and regulation.
2. The physiology of the carbohydrate Digestion in mammals
3. Illustrate the metabolism of carbohydrates through various anabolic and catabolic pathways like glycolysis, Kreb’s cycle, Glycogen metabolism, glucuronic acid cycle etc
4. Can understand about the disorders due to enzyme which works in metabolism process.
Suggested References:
Sr. References No.
1. Textbook of biochemistry by Rafi MD Nelson and Cox, LEHNINGER’s Principles of Biochemistry, KalyaniPublishers, Ludhiana/Worth Publishers, Inc., New York.
2. Biochemistry – Satyanarayan Life sciences fundamentals and practice part – I ,Pranav kumar and Usha mina
3. Review by physiological biochemistry- Haroid Harper Biochemistry – Lipin cott
On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US05CBCH24 Title of the HUMAN PHYSIOLOGY Course Total Credits Hours per 3 of the Course Week
Course 1. This course covers the physiology of humans, with emphasis on the major Objectives: organs and the processes they govern, including heart function and circulation, muscle function and movement and the kidney and osmoregulation. 2. Terminology, basic chemistry, cell structure and function, tissues, and the following systems: integumentary, skeletal, muscular, and nervous …
Course Content
Unit Description Weightage* (%) 1. INTRODUCTION TO PHYSIOLOGY 25% The cell and general physiology Functional organization of the human body and control of the internal environment Cells as the living units of the body Extracellular fluid unit of the internal environment Homeostatic mechanism of the major functional system Homeostasis Control system of the body 11 systems of human body
2. RESPIRATORY SYSTEM 25% Structure of the respiratory system The branching of airways from the trachea, the bronchial tree Phases of respiration Pulmonary ventilation External respiration Internal respiration Functions of respiratory system Transport of oxygen and carbon dioxide Oxygen-Hemoglobin dissociation curve Factors affecting the affinity of Hb for oxygen
(I) Acidity (II) PCo2 (III)Temperature (IV) BPG Blood buffers Acidosis -Alklosis
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
3. DIGESTIVE SYSTEM AND CIRCULATORY SYSTEM 25% Overview of the digestive system: gastrointestinal tract and the accessory digestive organs Basic principles of gastrointestinal absorption Absorption in the small intestine – absorption of water, ions and nutrients Importance of circulatory system A structure of human heart Cardiac cycle
4. MEMBRANE PHYSIOLOGY OF NERVE AND MUSCLE & 25% URINARY SYSTEMS Introduction and types of muscle Composition of skeletal muscle Mechanism of muscle contraction Structure and types of neuron cells Organization of nervous system Nerve impulse transmission Structure of kidney & nephron Urine formation GFR Renal threshold Tubular load Tubular maxima value (Tmax) Plasma clearance Renin angiotensin system
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Teaching- The teaching methodology consists of lectures, discussion, new technologies. Learning Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Course Outcomes: Having completed this course, the learner will be able to
1. The structure of major human organs and explain their role in the maintenance of healthy individuals.
2. The activities of organs are integrated for maximum efficiency.
3. The functional relationships between various organ systems of the body.
4. The anatomy and Physiology of cardiovascular and respiratory system and its disorders.
5. The Anatomy and Physiology of digestive ,nervous, urinary and reproductive system, endocrine system and sense organs and its disorders.
Suggested References:
Sr. References No.
1. Principles of Anatomy and Physiology- Gerard .J. Tortora and Bryn Derrickson
2. Textbook of Medical Physiology- Arthur. C .Guyton, John. E .Hall
3. Medical Physiology- Vol.1 and Vol.2-C.C.chattergy
On-line resources to be used if available as reference material
On-line Resources
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(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the ANALYTICAL US05CBCH26 Course BIOCHEMISTRY
Total Credits Hours per 3 of the Course Week
Course Objectives: 1. Interpret and critically evaluate analytical data and communicate the results of biochemical analyses in the form of formal scientific reports. 2. To introduce students to the base principles and theories of biochemical techniques.
3. Analyze the methods sections of primary research articles in biochemistry.
4. Evaluate and synthesise the biochemical literature in the context of contemporary and emerging analytical techniques.
5. To teach students basic laboratory skills and concepts of scientific method necessary for laboratory experiments.
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Course Content
Unit Description Weightage* (%)
1. Centrifugation Techniques Theory – definition and terminology General Principle and derivation for G and RCF Clinical, High speed and Ultra centrifuge analytical and preparative centrifuge; Centrifuge rotors: vertical, fixed angle, swinging bucket; Subcellular fractionation by differential centrifugation
2. Chromatographic Techniques Classification on the bases of phases. Types of Forces applied. definition General Principle and Theoretical plates Rf, Rg and Rx and other terminology used Principles, method and applications for following method a) Partition chromatography – types of paper chromatography and TLC. b) Adsorption chromatography c) Ion Exchange chromatography d) Molecular sieve chromatography e) Affinity chromatography f) Gas Liquid chromatography and g) High Performance Liquid Chromatography.
3. Electrophoretic Techniques Starch, Agarose, Polyacrylamide, Cellulose Acetate electrophoresis Difference between zone and free electrophoresis, factors affecting and uses, Horizontal and vertical units. Diagrammatic explanation and procedure to prepare separating unite for separation. General Principle, method and applications for following methods Paper electrophoresis .basic method to separate serum protein Gel electrophoresis. Types, function and composition of gels used. Starch, Agarose, Polyacrylamide, Cellulose Acetate electrophoresis Native and SDS – PAGE
4. Colorimetry and Spectrometry Beer Lambert’s law and derivation Definition for Transmittance, Absorbance, Optical density, ƛ max; Types of Spectroscopy: Principle, flow diagram, working & applications of a. Colorimeters b. Visible spectrometers c. UV spectrometers d. IR spectrometers e. fluorimeters
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Teaching- Learning Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Gain the knowledge and understanding the different techniques used in biochemistry
2. Understand the basic concepts and principles of biochemical techniques (centrifugation, chromatography, spectrophotometer, electrophoresis, manometer, etc)
3. Incorporate the basic laboratory skills and concepts in the conduct of any laboratory experiment
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Suggested References:
Sr. References No.
1. Biophysical chemistry- Principles and techniques- Upadhyay, Upadyay and Nath Himalaya Publication house Mumbai.
2. Principles and techniques of biochemistry&moleculer biology. Wison and Walker. Andreas Hofmann and Samuel clokie
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On-line resources to be used if available as reference material
On-line Resources
*****
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US05CBCH27 Title of the BASIC MICROBIOLOGY Course Total Credits Hours per 3 of the Course Week
Course 1. The course is to provide students with the basis to face the study of the Objectives: major fundamentals of microbiology including bacteriology, virology and immunology. 2. Knowledge acquired about prokaryotic and eukaryotic cellular processes, interaction of microorganisms. 3. Know various Culture media and their applications and also understand various physical and chemical means of sterilization. 4. Know General bacteriology and microbial techniques for isolation of pure cultures of bacteria, fungi and algae. …
Course Content
Unit Description Weightage* (%)
1. Introduction: Definition, Scope and History of Microbiology; 25% Differences between theprokaryotic and eukaryotic microorganisms; Basic understanding of classification of Bacteria, Viruses, Algae, Fungi and Protozoa.
2. Distribution of Microorganisms: 25% a) In soil – distribution of bacteria, molds, yeast and protozoa. b) In Water – Quality of drinking water, Bacteriological examination of water Totalbacterial count for E. coli; Purification of water: filtration, sedimentation andaddition of chemicals.
3. a) Microorganism in air, air borne diseases. 25% b) Microorganisms in milk and milk products. Preservation of milk, milk borne diseases. c)Principles and methods of Sterilization and disinfection
4. Microbial growth, growth rate, doubling time, exponential growth phases, 25% factors affecting growth – nutrient factors (C, O, N, P, S trace elements) and non-nutrients (temperature, hydrostatic pressure, pH, osmotic strength) for the microorganism.
...
Teaching- The course will be covered through lectures, with the help of new technologies Learning Apart from discussions on topics covered in lectures, assignments will also be Methodology given
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understand the interactions between viruses and the host immune system • the terms Oncogenes and tumor suppressor genes.
2. How tumor viruses interact with these products and their intersecting pathways and cause oncogenesis
3. Vaccine strategies and mechanisms of antiviral drugs and interferons
4. Know how viruses can be used as tools to study biological processes , as cloning vectors and for gene transfer
Suggested References:
Sr. References No.
1. Microbiology – M.J. Pelzar, Jr. Et al. McGraw Hill
2. Text book of Microbiology-Ananathanarayanan and Panikar Orient Long.
3. Microbiology-Lansing M. Prescott IV edition, McGraw-Hill.
On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the US05CBCH25 BIOCHEMISTRY PRACTICAL Course Total Credits Hours per 8 4 of the Course Week
Course Student should be able to: Objectives: 1. To assume, inquire and analyse, apply logical principles, validate assumptions, solve problems, integrate knowledge and widen perspective. To promote originality of ideas. 2. Able to use the biochemical techniques, instruments. 3. Importance of bio molecules in living cells. 4. Chemistry of bio molecule.
Course Content
Unit Description Weightage* (%) 1. (BIOCHEMISTRY PRACTICAL) 100 • Use of analytical balance and weighting, Use of Colorimeter, spectrophotometer, Calibration of Volumetric flasks, pipettes. Calculation, preparation of normal, molar and percentage solutions. • Chromosome staining by gimsa stain Determination of Cot value and GC content of DNA • Estimation of DNA by DPA method • Extraction of protein from plant (Green gram) • Study of normal composition of Urine • Study of abnormal constituents of Urine • Estimation of haemoglobin by cyanmethemoglobin method (Drabkin method) • Study of erythrocyte sedimentation rate (ESR) • Estimation of total carbohydrates by anthrone method • Estimation of starch by DNS method • Estimation of total protein Bradford method • Separation of amino acids by TLC • Determination of saponification of given oil • Separation of plant pigments by Paper Chromatography • Effect of the Enzyme (Invertase) concentration on enzyme activity
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
• Effect of substrate concentration on Enzyme activity • Effect of time on enzyme activity • Effect of pH on enzyme activity
Teaching- Practical sessions will be conducted in a suitably equipped laboratory Learning either individually or in groups depending on the nature of exercise as well Methodology as availability of infrastructure. The module will consist of seminars, in which the students will be supported in their practical teaching classes This practical session will also involve them observing their peers and participating in observation tasks and oral feedback. There will be a focus on particular experiment perform by students.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understanding of bio chemical analysis and estimation of various bio molecules.
2. An ability to acquire in-depth practical knowledge of Biochemistry and the ability to apply the acquired knowledge to provide cost efficient solutions in Biochemistry.
3. Ability to conduct experiment, analyze and interpreted the results.
4. Able to calculate the experimental calculations.
Suggested References:
Sr. References No.
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
1. Standard methods of biochemical analysis – s.r. thimmaiah.
On-line resources to be used if available as reference material
On-line Resources
Related References Practical Books
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the
Course GENETIC ENGINEERING
US06CBCH21 Total Credits Hours per 3 of the Course Week
Course The objective of the course is to familiarize the students with the basic Objectives: concepts in genetic engineering. 1. Process of inserting new genetic information into existing cells in order to modify a specific organism for the purpose of changing its characteristics. 2. To acquaint the students to versatile tools and techniques employed in genetic engineering and recombinant DNA technology; and to appraise them about applications genetic engineering.
Course Content
Unit Description Weightage* (%) 1. 25% Regulatuion of gene expression 1. Principle of gene regulation 2 .Definition, constitutive enzyme, induced enzyme .inducible and induction, repressible and repression, activators and repressors 3. Lac operon hypothesis A. Negative control mechanism B. Positive control mechanism 4. Tryptophan operon A. Repressor mechanism B. Attenuation mechanism 5. Regulation of gene expression in eukaryotes A. Euchromatin and heterochromatin B. Many eukaryotic promoters are positively regulated C. DNA binding activators and co activators
2. 25% Gene mutation and repair Definition : mutation and repair 1. Mutation hotspot ,mutagens 2. Mutagenic agents
A. Base analogues B. Agents modifying nitrogen base C. Agents producing distortion in DNA
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
3. The primary mutagenic effect of UV light 4. DNA Repair mechanism A. Mismatch Repair B. Base expression repair C. Nucleotide excision repair D. Direct repair
3. Recombinant DNA technology and genetic engineering 25% 1. Purification of DNA
A. Preparation of total cell DNA B. Preparation of plasmid DNA 2. Gene cloning vectors (brief) A. Plasmid B. Lambda phase C. Cosmid D. Ti plasmid E. PBR322 3. Restriction endonuclease its types and function
4. Applied genetics 25% 1. Detection of recombinant clone A. Southern blotting B. Northern blotting C. Western blotting 2. Principle, method and application of A.PCR B.RFLP C. DNA finger printing D. gene library 3. Gene sequencing A. Sanger’s method B. Maxam and Gilbert’s method
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Teaching- The course will be covered through lectures, with the help of new technologies Learning Apart from discussions on topics covered in lectures, assignments will also be Methodology given.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
Page 2 of 4
SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. The student will gain a basic understanding on human genetics and hereditary.
2. They learn about DNA, RNA and their replication, mutations, DNA repair mechanism.
3. Students learn about transgenic animal, their application in pharmaceutical industry, cloning and its importance
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Suggested References:
Sr. References No.
1. Freifelder’s Essentials of Molecular Biology by George M. Malacinski
2. Molecular Biology of the Gene 6th edition. By Watson J D, Baker T.A., Bell, S. P., Gann, A., Levine, M., and Losick, R.,2008 Cold Spring Harbour Lab. Press, Pearson Pub.
3. Gene Cloning & DNA Analysis: An introduction by T A Brown WILEY Blackwell 7th Edition
4. Lewin’s Gene XII by Elliot S Goldstein, Jocelyn E. Krebbs, and Stephen T. Kilpatrick
On-line resources to be used if available as reference material
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US06CBCH22 Title of the BASIC IMMUNOLOGY Course Total Credits Hours per 3 of the Course Week
Course 1. The students will be introduced to the basic concepts of immunology as Objectives: it relates to human and animal health. 2. The students will be able to describe the roles of the immune system in both maintaining health and contributing to disease. 3. The students will be able to transfer knowledge of immunology into clinical decision-making through case studies presented in class. …
Course Content
Unit Description Weightage* (%) 1. Overview and historical perspective of Immunology: Immunity, Types: 25% innate and acquired - active, passive, natural and artificial immunity. Overview of immune system, Cells of the immune system and functions, Organs of the immune systems and functions - primary and secondary lymphoid organs. 2. Antigens: Nature and types of antigens, specificity, epitope, haptens, 25% adjuvants, immunogenicity, factors affecting immunogenicity. Antibodies: Immunoglobulins-Structure, Classes and functions; Antigens-antibody reactions - Agglutination, precipitation, complement fixation, neutralization; Immunofluorescence. 3. Immunodiagnostic technique: Single radial Immunodiffusion, Double 25% Immunodiffusion, Immunoelectrophoresis, Rocket electrophoresis, Haemaggulitination, bacterial agglutination, ELISA, RIA. 4. Hypersensitivity reactions: Type I, II, III and IV, Allergy and 25% inflammation; Fundamentals of autoimmune disorders, Immunodeficiency and Immune suppression disease. Transplantation Immunology: graft acceptance and rejection
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Teaching- Lectures and colloquiums Learning Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Apply basic techniques for identifying antigen antibody interactions
2. Exemplify the adverse effect of immune system including Allergy, hypersensitivity and autoimmunity
3. Design a model of Immunoglobulin
4. Elucidate the reasons for immunization and aware of different vaccination
Suggested References:
Sr. References No.
1. Textbook of Immunology- Chakkaravarthy, Tata McGraw Hill publishing Company, Ltd (2004)
2. Essentials of Immunology- I. Roitt, Blackwell Science
3. Immunology Textbook by Janis Kuby
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
4. Textbook of Microbiology by R. Ananthanarayan
On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US06CBCH23 Title of the HUMAN METABOLISM-II Course Total Credits Hours per 3 of the Course Week
Course 1. The major catabolic and anabolic pathways by which human cell types Objectives: metabolize protein and nucleotides. 2. The key regulatory points in human metabolic pathways. 3. How diet and hormonal signalling regulate major human metabolic pathways. 4. To recognize the role of vitamins and minerals in intermediary metabolism. 5. To define the biochemical functions and integrated metabolism of major tissues including brain, digestive system, liver, red cell, muscle and adipocyte. 6. To explain the molecular mechanisms underlying the major inherited diseases of metabolism. 8. To explain how certain medicines and drugs impinge upon metabolic pathways.
Course Content
Unit Description Weightage* (%)
1. RESPIRATORY CHAIN AND OXIDATIVE PHOSPHORYLATION 25% Functional stages of Respiratory chain Components of respiratory chain 1. Nicotinamide nucleotides 4. Coenzyme Q 2. Flavin nucleotides 5. Cytochromes 3. Iron-Sulphur proteins Organisation of Electron Transport Chain Inhibitors of electron transport chain Oxidative phosphorylation Mechanism of oxidative phosphorylation (Hypothesis) P:O Ratio ATP synthase Inhibitors of oxidative phosphorylation Uncouplers Brown adipose tissue
2. PROTEIN METABOLISM 25% Digestion and absorption of protein Cellular uptake of Amino acid Metabolic uses of Amino acid Protein turnover Amino acid pool Transamination & Deamination Ammonia transport and metabolism
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Ammonia toxicity Removal of ammonia Metabolism of individual amino acid (In brief) Urea cycle Metabolic disorder of protein Phenylketonuria Albinism
3. NUCLEIC ACID METABOLISM 25% Biosynthesis of Purine Nucleotides De Novo synthesis Synthesis of AMP and GMP from IMP Formation of Purine Nucleotide Diphosphates and Triphosphates Inhibitors of Purine synthesis Regulation of Purine Nucleotide biosynthesis Salvage pathways Synthesis of Doexyribonucleotides Degradation of Purine Nucleotides Uric acid metabolism Gout Pyrimidine metabolism De Novo synthesis , Regulation , Degradation of pyrimidine nucleotides
4. INTEGRATION OF METABOLISM 25% Energy demand and supply Integration of major metabolic pathways Regulation of metabolic pathways Organ specialization metabolic integration in Liver Adipose tissue Skeletal muscle Brain Fast – feed cycle The Fed state The Fasting state The Fe-fed state Metabolism in starvation
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Teaching- This course will be taught by a mixture of discussion, lecture, readings, smart - Learning board technology, on-line quizzes and student presentation modalities. Methodology
Evaluation Pattern
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understand the structure and metabolism of macromolecules and understand the regulation and disorders of metabolic pathways.
2. Acquire thorough knowledge in biochemical human metabolism.
3. Nucleic acids and proteins are metabolized, emphasizing the role of few intermediates of their metabolism, monitoring the deficiency and abundance disorders of amino acid metabolisms and the role of enzymes in the regulation of the pathways
4.
Suggested References:
Sr. References No.
1. Textbook of biochemistry by Rafi MD
2. Principle of biochemistry – Lehninger
3. Life sciences fundamentals and practice part – I ,Pranav kumar and Usha mina
4. Review by physiological biochemistry- Haroid Harper
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
5. Biochemistry – Lipin cott Human metabolism by Michael Palmer
On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US06CBCH24 Title of the CLINICAL BIOCHEMISTRY Course Total Credits Hours per 3 of the Course Week
Course 1. Explain the basic elements of core Biochemistry and specialized tests of Objectives: biochemistry. 2. Clinical Biochemistry aims to initiate the student in understanding the study of the biological properties in vitro that contribute to the prevention, diagnosis, prognosis and monitoring of diseases and disease states in humans. …
Course Content
Unit Description Weightage* (%) 1. BLOOD AND BODY FLUIDS 25% Clinical biochemistry: definition Water and electrolytes : distribution of water In body , water balance in human body, electrolytes distribution and balance in body fluid , regulation of water and electrolytes balance Blood: Normal constituents of blood and their variation in pathological conditions - urea, uric acid, creatinine, glucose, bilirubin, total protein, albumin/globulin ratio. Lipid profile – cholesterol, triglycerides, lipoproteins - HDL and LDL. functions , physical characteristics , composition , functions of blood cells (RBC , WBC , PLATELETS) Body fluids: major body fluids , normal composition and functions of CSF , Lymph , saliva , urine
2. PLASMA PROTEINS 25% Plasma protein : types and major functions of plasma proteins Separation of plasma protein Chemistry , functions and clinical significance of 1. Albumin 2. Alpha-1-antitripsin 3. Lipoproteins 4. Haptoglobin 5. Ceruloplasmin 6. Transferrin Prothrombin Fibrinogen Lipid profile
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
3. ORGAN FUNCTION TEST AND DISEASE 25% Liver function test : functions of liver, classification of liver function test Renal function test : functions of kidney , classification of renal function test Jaundice : types of jaundice Fatty liver Diabetes mellitus : WHO classification , clinical symptoms , biochemical symptoms , life threatening complications (CHD , Retinopathy , nephropathy ,neuropathy Nephritis Cancer
4. HEMOGLOBIN, ANEMIA AND BLOOD COAGULATION 25% Chemistry functions and structure of haemoglobin Normal types of haemoglobin Properties of haemoglobin Derivatives of haemoglobin Types of anemia Blood coagulation Basic reaction (some properties and functions of the principal of clotting factors) Role of blood platelets Biochemical reactions in the clotting process Anticoagulants Fibrinolytic system
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Teaching- The teaching methodology consists of lectures, discussion, new technologies. Learning Methodology
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Course Outcomes: Having completed this course, the learner will be able to
1. Can understand the pathophysiology and molecular basis of the most prevalent diseases.
2. Know the analytical methods commonly used in the clinical laboratory.
3.
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Suggested References:
Sr. References No.
1. Principles of Anatomy and Physiology- Gerard .J. Tortora and Bryn Derrickson
2. Textbook of Medical Physiology- Arthur. C .Guyton, John. E .Hall.
3. Medical Physiology- Vol.1 and Vol.2-C.C.chattergy
4. Human biochemistry by James M. Orten & Otto W. Neuhaus
5. Textbook of biochemistry for medical students :Rafi MD
On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US06CBCH26 Title of the CLINICAL ENDOCRINOLOGY Course Total Credits Hours per 3 of the Course Week
Course 1. This is a comprehensive study of the endocrine system which will allow Objectives: the student to integrate and better understand the functions of the other systems of the body. 2. The relationship of the nervous system to the endocrine system is explored in the context of signaling within a multicellular organism. 3. Understand the function of the endocrine organs, metabolism of their hormones, and their effects on the body. 4. Interpret the results of measurements of stimulation and suppression of glands. 5.Know the pharmacology and use of insulin, thyroid hormones, corticosteroids, androgens, estrogens, vasopressin, and other agents
Course Content
Unit Description Weightage* (%) 1. CLASSIFICATION OF HORMONES 25% Definition General importance Mechanism of action of Group- I Hormone Mechanism of action of Group- II Hormone Hypothalamic hormone Regulatory feedback loops between hypothalamus pituitary glands
2. EFFECT OF HORMONES ON METABOLISM AND DISORDERS 25% Growth hormone Thyroid hormone Insulin Glucagon Cortisol Biochemical and physiological function of Catecholamine
3. DISORDERS OF HORMONES 25% Goitre Hyperthyroidism Hypothyroidism Thyroid function test Diabetes mellitus Hypoglycaemia Acromegaly
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Gigantism
4. HORMONES OF ENDOCRINE GLANDS WITH THEIR 25% PHYSIOLOGICAL FUNCTIONS Endocrine and Exocrine glands Hormones of pituitary gland Hormones of Thyroid gland Hormones of Pancreas Hormones of Adrenal gland
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Teaching- Presentations will make use of traditional teaching tools as well as Learning assorted electronic resources. Different teaching-learning methods have Methodology been used.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. The student will demonstrate an understanding of the anatomy of the endocrine system.
2. An understanding of the basic properties of hormones
3. Understand the role of hormones in maintaining body function.
4. Knowledge of the major endocrine disorders
Suggested References:
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Sr. References No.
1. Textbook of biochemistry by Rafi MD
2. Biochemistry – Satyanarayan
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On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code US06CBCH27 Title of the PLANT BIOCHEMISTRY Course Total Credits Hours per 3 of the Course Week
Course 1. The course is intended for students in the plant sciences. Topics are taught in Objectives: the context of plant biology. 2. Successful completion of this course will provide students with fundamental knowledge of biochemistry and specific knowledge of compounds and biochemical pathways that occur in plants. …
Course Content
Unit Description Weightage* (%) 1. Plant cell Structure 25% Biochemistry of Specialized Plant cell Organelles , Primary and Secondary cell wall, Plasmodesmata ( structure and Function ), Water relations of plants: Role of water, Absorption, Adsorption, Conduction and Transpiration, Guttation, Water Balance and Stress. 2. Secondary Metabolism 25% Secondary Metabolites-Phenols, Tannins, Lignin, Flavonoids, Waxes, Cutin and Suberin-structures and functions 3. Photosynthesis 25% Hill’s Reaction, Light Reaction, Dark Reaction C3 and C4 cycles, Photo Respiration, Factors affecting rate of Photosynthesis 4. Plant Hormones 25% Auxins, Cytokinins, Gibberalic acids, Absisic acid, Brassinosteroids, Salicylic acid, Jasmonic acid.
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Teaching- Classroom teaching, problem solving, PowerPoint presentations, Flow Charts, Learning animated videos. Methodology
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. Understand plant cell structure, organization, and apply specific biochemical functions to all compartments of the plant cell.
2. Understand how light energy is captured and used to provide chemical forms of energy to power the functions of cells and whole plants. The importance of CO2 fixation and carbohydrate metabolism will be presented. The nature and composition of plant cell walls will be explored.
3. They will get familiarize with major experiments and discoveries that influenced the development
4. Understand the plant-specific components, and their functional significance at multiple level
Suggested References:
Sr. References No.
1. Plant Physiology by Salis burry and Ross
2. Plant Biochemistry by Hans - Walter Heldt
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
On-line resources to be used if available as reference material
On-line Resources
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
(Name of the Degree) (Programme Name) (Degree abbreviation) (Programme Name) Semester (Use Roman numerals)
Course Code Title of the BIOCHEMISTRY US05CBCH25 Course PRACTICAL Total Credits Hours per 8 4 of the Course Week
Course 1. An ability to apply fundamental knowledge related to pure sciences in an Objectives: interdisciplinary manner for providing innovative solutions to need based problems for global impact. 2. Students shall be able to conduct the clinical biochemistry, Diagnostic biochemistry experiments as well as to analyze and interpret the results. 3. Students shall be able to use the biochemical techniques, Genetic Engineering & Biotechnology skills and modern pathological tools necessary for professional practice and for research
Course Content
Unit Description Weightage* (%) 1. (BIOCHEMISTRY PRACTICAL) 100 • Estimation of Creatinineby Jaffe method • Estimation of protein by Lowery method • Estimation of SGOT and its clinical importance • Estimation of SGPT and its clinical importance • Estimation of A/G ratio • Estimation of Urea • Estimation of serum bilirubin • Estimation of serum cholesterol • Determination of human blood group • Determination of Rh factor • RA test • Widal test • Estimation of DNA by UV spectroscopy • Demonstration of Polyacrylamide gel electrophoresis of serum proteins
Teaching- Practical sessions will be conducted in a suitably equipped laboratory Learning either individually or in groups depending on the nature of exercise as well Methodology as availability of infrastructure. The module will consist of seminars, in
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
which the students will be supported in their practical teaching classes This practical session will also involve them observing their peers and participating in observation tasks and oral feedback. There will be a focus on particular experiment perform by students.
Evaluation Pattern
Sr. Details of the Evaluation Weightage No.
1. Internal Written / Practical Examination (As per CBCS R.6.8.3) 15%
2. Internal Continuous Assessment in the form of Practical, Viva-voce, 15% Quizzes, Seminars, Assignments, Attendance (As per CBCS R.6.8.3)
3. University Examination 70%
Course Outcomes: Having completed this course, the learner will be able to
1. An ability to use the techniques, skills and modern professional tools necessary for professional practice and for research.
2. Gain knowledge of clinical biochemistry as well as pathological tests.
Suggested References:
Sr. References No.
1. Standard methods of biochemical analysis – s.r. thimmaiah.
On-line resources to be used if available as reference material
On-line Resources
Related References Practical Books
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SARDAR PATEL UNIVERSITY Vallabh Vidyanagar, Gujarat (Reaccredited with ‘A’ Grade by NAAC (CGPA 3.25) Syllabus with effect from the Academic Year 2021-2022
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