For Research Use Only

CEP250/CNAP1 Polyclonal antibody www.ptglab.com Catalog Number:14498-1-AP 21 Publications

Catalog Number: GenBank Accession Number: Purification Method: Basic Information 14498-1-AP BC001433 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 800 μg/ml by 11190 WB 1:500-1:1000 Nanodrop and 460 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:1000 method using BSA as the standard; centrosomal 250kDa for WB Source: IHC 1:50-1:500 Calculated MW: IF 1:50-1:500 Rabbit 281 kDa Isotype: Observed MW: IgG 250 kDa Immunogen Catalog Number: AG5925

Applications Tested Applications: Positive Controls: IF, IHC, IP, WB,ELISA WB : HeLa cells, Cited Applications: IP : HeLa cells, IF, WB IHC : human placenta tissue, Species Specificity: human IF : HeLa cells, Cited Species: human, mouse Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0

CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and Background Information daughter . It is required for -centriole cohesion during interphase of the . It dissociates from the when parental centrioles separate at the beginning of . The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the .

Notable Publications Author Pubmed ID Journal Application Yuki Yoshino 32788231 J Cell Sci IF Biao Wang 33987909 EMBO Rep IF Johan Busselez 31076588 Sci Rep

Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage

For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data

HeLa cells were subjected to SDS PAGE followed by Immunofluorescent analysis of (-20 Ethanol) fixed Immunohistochemical analysis of paraffin- western blot with 14498-1-AP (CEP250/CNAP1 HeLa cells using 14498-1-AP (CEP250/CNAP1 embedded human placenta tissue slide using antibody) at dilution of 1:500 incubated at room antibody) at dilution of 1:50 and Alexa Fluor 488- 14498-1-AP (CEP250/CNAP1 antibody at dilution of temperature for 1.5 hours. conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1:200 (under 10x lens).

Immunohistochemical analysis of paraffin- IP Result of anti-CEP250/CNAP1 (IP:14498-1-AP, embedded human placenta tissue slide using 5ug; Detection:14498-1-AP 1:500) with HeLa cells 14498-1-AP (CEP250/CNAP1 antibody at dilution of lysate 1200ug. 1:200 (under 40x lens).