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CEP250/CNAP1 Polyclonal Antibody Catalog Number:14498-1-AP 21 Publications

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CEP250/CNAP1 Polyclonal Antibody Catalog Number:14498-1-AP 21 Publications For Research Use Only CEP250/CNAP1 Polyclonal antibody www.ptglab.com Catalog Number:14498-1-AP 21 Publications Catalog Number: GenBank Accession Number: Purification Method: Basic Information 14498-1-AP BC001433 Antigen affinity purification Size: GeneID (NCBI): Recommended Dilutions: 150ul , Concentration: 800 μg/ml by 11190 WB 1:500-1:1000 Nanodrop and 460 μg/ml by Bradford Full Name: IP 0.5-4.0 ug for IP and 1:500-1:1000 method using BSA as the standard; centrosomal protein 250kDa for WB Source: IHC 1:50-1:500 Calculated MW: IF 1:50-1:500 Rabbit 281 kDa Isotype: Observed MW: IgG 250 kDa Immunogen Catalog Number: AG5925 Applications Tested Applications: Positive Controls: IF, IHC, IP, WB,ELISA WB : HeLa cells, Cited Applications: IP : HeLa cells, IF, WB IHC : human placenta tissue, Species Specificity: human IF : HeLa cells, Cited Species: human, mouse Note-IHC: suggested antigen retrieval with TE buffer pH 9.0; (*) Alternatively, antigen retrieval may be performed with citrate buffer pH 6.0 CEP250, also known as C-Nap1, is a 250 kDa coiled-coil protein that localizes to the proximal ends of mother and Background Information daughter centrioles. It is required for centriole-centriole cohesion during interphase of the cell cycle. It dissociates from the centrosomes when parental centrioles separate at the beginning of mitosis. The protein associates with and is phosphorylated by NIMA-related kinase 2, which is also associated with the centrosome. Notable Publications Author Pubmed ID Journal Application Yuki Yoshino 32788231 J Cell Sci IF Biao Wang 33987909 EMBO Rep IF Johan Busselez 31076588 Sci Rep Storage: Storage Store at -20°C. Stable for one year after shipment. Storage Buffer: PBS with 0.02% sodium azide and 50% glycerol pH 7.3. Aliquoting is unnecessary for -20ºC storage For technical support and original validation data for this product please contact: This product is exclusively available under Proteintech T: 1 (888) 4PTGLAB (1-888-478-4522) (toll free E: [email protected] Group brand and is not available to purchase from any in USA), or 1(312) 455-8498 (outside USA) W: ptglab.com other manufacturer. Selected Validation Data HeLa cells were subjected to SDS PAGE followed by Immunofluorescent analysis of (-20 Ethanol) fixed Immunohistochemical analysis of paraffin- western blot with 14498-1-AP (CEP250/CNAP1 HeLa cells using 14498-1-AP (CEP250/CNAP1 embedded human placenta tissue slide using antibody) at dilution of 1:500 incubated at room antibody) at dilution of 1:50 and Alexa Fluor 488- 14498-1-AP (CEP250/CNAP1 antibody at dilution of temperature for 1.5 hours. conjugated AffiniPure Goat Anti-Rabbit IgG(H+L). 1:200 (under 10x lens). Immunohistochemical analysis of paraffin- IP Result of anti-CEP250/CNAP1 (IP:14498-1-AP, embedded human placenta tissue slide using 5ug; Detection:14498-1-AP 1:500) with HeLa cells 14498-1-AP (CEP250/CNAP1 antibody at dilution of lysate 1200ug. 1:200 (under 40x lens)..
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