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sigma-aldrich.com By David H. Hall, Albert Einstein College of Medicine, New York; Zeynep F. Altun, Albert Einstein College of Medicine, New York erived from the acclaimed online “WormAtlas,” C. elegans Atlas is a Dlarge-format, full-color atlas of the hermaphroditic form of the model organism C. elegans, known affectionately as “the worm” by workers in the field. Prepared by the editors of the Wormatlas Consortium, David H. Hall and Zeynep F. Altun, this book combines explanatory text with copious, labeled, color illustrations and electron micrographs of the major body systems of C. elegans. Also included are electron microscopy cross sections of the worm. This laboratory reference is essential for the working worm biologist, at the bench and at the microscope, and provides a superb companion to the C. elegans II monograph. It is also a valuable tool for investigators in the fields of developmental biology, neurobiology, reproductive biology, gene expression, and molecular biology. 2008, 348 pp., illus., appendix, index Concealed wire binding $125 ISBN 978-087969715-0 Hardcover $175 ISBN 978-087969794-5

CONTENTS 1. Introduction to C. elegans Anatomy Part I. Overview 2. Epithelial System Part II. The Pharynx Part I. Hypodermis (Epidermis) Part III. The Intestine Part II. Seam Cells Part IV. The Rectum and Anus Part III. Interfacial Epithelial Cells 8. Reproductive System Part IV. Atypical Epithelial Cells Part I. Overview 3. Nervous System Part II. The Somatic Gonad Part I. General Description Part III. The Germ Line Part II. Neuronal Support Cells Part IV. The Egg-laying Apparatus 4. Excretory System 9. The Cuticle 5. Muscle System 10. Pericellular Structures Part I. Muscle Appendix: Transverse Thin Sections of the Adult Hermaphrodite C. elegans Part II. GLR Cells Index Part III. Head Mesodermal Cell 6. Coelomocyte System 7. Alimentary System

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Figure 2. In Situ hybridization analysis of FFPE breast cancer tissue using References: miRCURY LNA™ detection probes. Upper panel: stained with Digoxigenin- 1. Miska, E.A. How microRNAs control cell division, differentiation and labeled nuclear localization probe. Lower panel: stained with Digoxigenin- death. Curr. Opin. Genet. Dev. 15, 563–568 (2005). labeled miR-21-specifi c probe. Detection was done with anti-Digoxigenin- 2. Calin, G. A. et al. Human microRNA genes are frequently located at fragile sites and genomic regions involved in cancers. Proc. Natl. Acad. AP followed by NBT/BCIP chromogene substrate. Sci. USA 101, 2999–3004 (2004). The miR-21 is expressed most strongly in fi broblast-like cells close to 3. Esquela-Kerscher, A. & Slack, F. J. Oncomirs—microRNAs with a role tumor cells. in cancer. Nat. Rev. Cancer 6, 259–269 (2006). 4. Calin GA, Croce CM. MicroRNA signatures in human cancers. Nat Rev miRCURY LNA™ microRNA PCR system Cancer 2006;6:857-66. Real-time PCR is one of the standard methods for detection 5. Calin GA, Ferracin M, Cimmino A, et al. A microRNA signature and quantitation of gene expression in small samples. associated with prognosis and progression in chronic lymphocytic leukemia. N Engl J Med 2005; 353:1793-801. However, for detection of microRNA, conventional DNA- 6. Yanaihara N, Caplen N, Bowman E, et al. Unique microRNA molecular based PCR techniques are often not suffi ciently sensitive profi les in lung cancer diagnosis and prognosis. Cancer Cell and specifi c. Conventional DNA-based primers will often 2006;9:189-98. 7. Castoldi, M. et al. A sensitive array for microRNA expression profi ling be equivalent in size to the full-length microRNA target, (miChip) based on locked nucleic acids (LNA). RNA 12, 913–920 (2006). which introduces great risk of primer-dimer formation. 8. Kloosterman, W.P. et al. In Situ detection of miRNAs in animal embryos Taking advantage of the LNA™ technology, the miRCURY using LNA-modifi ed oligonucleotide probes. Nat. Methods 3, 27–29 LNA™ microRNA PCR system makes it possible to use very (2006). 9. Raymond, C.K. et al. Simple, quantitative primer-extension PCR assay short and yet microRNA-specifi c primers without sequence for direct monitoring of microRNAs and short-interfering RNAs. RNA. overlap, thereby minimizing the risk of primer-dimer 2005 November; 11(11): 1737–1744. formation. This results in extremely sensitive assays with a 10. Iorio, M.V. et al. MicroRNA gene expression deregulation in human breast cancer. Cancer Res. 65, 7065–7070 (2005). dynamic range of over eight orders of magnitude. The assays are optimized for microRNA quantitation from 10 pg of total RNA and can reliably detect as few as 10 microRNA copies. Log on to Exiqon.com • Get free Nature microRNA booklets Oncotech is a leading molecular oncology company • View a 3-D animation of the research benefi ts with complete support for cancer drug development of our LNATM technology and clinical studies • Fill in a few blanks about your research needs Oncotech is the leader in cancer drug resistance testing in on exiqon.com/contact the United States. The Extreme Drug Resistance (EDR) Assay • A new online source of trusted techniques in molecular and cellular biology • Contains cutting-edge and classic protocols presented step-by-step with cautions and troubleshooting • Frequently updated and annotated • Interactive, customizable, and fully searchable

Subject Coverage Antibodies Bioinformatics/Genomics old Spring Harbor Laboratory is renowned for its teaching of biomedical Cell Biology research techniques. For decades, participants in its celebrated, hands-on courses Chromatography C and users of its laboratory manuals have gained access to the most authoritative and Computational Biology DNA Delivery/Gene reliable methods in molecular and cellular biology. Now that access has moved online. Transfer Visit Cold Spring Harbor Protocols today and discover a rich, interactive source of new Electrophoresis and classic research techniques. The site is fully searchable, with many tools that can Genetics be customized by users, including topic-based alerting and personal folders. Through High Throughput Analysis a web-based editorial process, users also have the opportunity to add refereed com- Imaging/Microscopy ments to each protocol. Links in the online protocols offer additional resources, and Immunology step-by-step instructions print out in a convenient form, complete with materials, Laboratory Organisms cautions, and troubleshooting advice. Each protocol is citable and presented and edit- Molecular Biology ed in the style that has made Molecular Cloning, Antibodies, Cells, and many other Neuroscience Cold Spring Harbor manuals essential to the work of scientists worldwide. The Newly Added Protocols current collection of more than 1000 protocols is continuously expanded, updated, Plant Biology and annotated by the originators and users of the techniques. Polymerase Chain Reaction (PCR) CSH Protocols is created by Cold Spring Harbor Laboratory Press in association with Proteins and Proteomics HighWire Press of Stanford University. RNA Interference (RNAi)/siRNA Cold Spring Harbor Protocols Stem Cells Executive Editor: Dr. David Crotty • ISSN 1559-6095 / online, monthly Transgenic Technology Available exclusively via institutional site license

For pricing information or to request a free trial, visit the website or: Phone:1-800-843-4388 (Continental US and Canada) or 516-422-4100 (all other locations) Fax: 516-422-4097 E-mail:[email protected] or Website: www.cshlpress.com Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924

Edited by Jan A. Witkowski and John R. Inglis, Cold Spring Harbor Laboratory n 1911, the influential geneticist Charles Davenport published Heredity in Relation to IEugenics, advancing his ideas of how genetics would improve society in the 20th century. It became a college textbook and a foundation for the widespread eugenics movement in the United States. Nearly 100 years later, many of the issues raised by Davenport are again being debated, in different guises. In this new volume, prominent academics discuss themes from Davenport’s book—human genetic variation, mental illness, nature vs. nurture, human evolution—in a contemporary context. Davenport’s original book is reprinted along with the essays. This book will be useful to historians of science as well as those interested in the social implications of human genetics research—past, present, and future. 2008, 490 pp., illus. Hardcover $55 ISBN 978-087969756-3

CONTENTS Preface Psychiatric Genetics in an Era of Relative Jan A. Witkowski and John R. Inglis, Cold Spring Enlightenment Harbor Laboratory Daniel R. Weinberger,National Institute of Mental Foreword Health, and David Goldman, National Institute of Matt Ridley, Newcastle-upon-Tyne Alcohol Abuse and Alcoholism Genes and Politics Genes in Mind? James D. Watson, Cold Spring Harbor Laboratory Lindsey Kent, St. Andrews University, and Simon Baron-Cohen, Cambridge University Charles Benedict Davenport, 1866—1944 Davenport and Heredity Counseling Jan A. Witkowski, Cold Spring Harbor Laboratory Philip R. Reilly, Interleukin Genetics The Eugenic World of Charles Benedict Davenport Genetics and Equality Elof A. Carlson, University at Stony Brook Ronald Dworkin, New York University School of Law Davenport’s Dream Genetics and Human Nature Maynard V. Olson, University of Washington Lewis Wolpert, University College London Genetic Determinism and Evolutionary Ethics: AMitochondrial Perspective A Reprint of Heredity in Relation to Eugenics Douglas C. Wallace, University of California, Irvine Charles Benedict Davenport (Published by Henry Holt and Company, New York, 1911)

To order or request additional information, please visit our Website or: Call: 1-800-843-4388 (Continental US and Canada) 516-422-4100 (All other locations) FAX: 516-422-4097 E-mail: [email protected] Write: Cold Spring Harbor Laboratory Press, 500 Sunnyside Blvd., Woodbury, NY 11797-2924 IN THE FIELD OF MICE ONE COMPANY STANDS OUT

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CONGRESS PRESIDENT CO-CHAIRS INTERNATIONAL PROGRAM COMMITTEE PLENARY LECTURERS (confirmed) Rudi Balling Charles Langley (UC Davis, USA) Richard Axel (USA) Eric Lander (USA) (HZI Braunschweig, Germany) Rudi Balling (HZI Braunschweig, Germany) Elizabeth Blackburn (USA) Christiane Nüsslein-Volhard (Gemany) Mario Capecchi (USA) Svante Pääbo (Germany) CONGRESS SECRETARY GENERAL HONORARY PRESIDENTS: Rudolf Jaenisch (USA) Phillip Sharp (USA) Alfred Nordheim Rudolf Jaenisch Antoine Kremer (France) Oliver Smithies (USA) (Tübingen University, Germany) Christiane Nüsslein-Volhard Tomoko Ohta

IN PARTNERSHIP WITH International Genetics Federation (IGF)

By Elof Axel Carlson, Emeritus, State University of New York at Stony Brook raditional views of human nature focus on the supernatural, defining us as Tcreatures with souls, minds, and spirits that transcend our physical attrib- utes. In this provocative book, distinguished scientist and historian Elof Axel Carlson argues for a different understanding of ourselves based on our biology— cellular organization, genetics, life cycle, evolution, and our origins as a species. This interpretation does not negate our capacity for imagination, spiritual and emotional yearnings, or aesthetic appreciation for art, music, and literature. Carlson challenges educators, the media, and public policy makers to integrate the evidence from science more fully into our understanding of ourselves. 2008, 180 pp., index Hardcover $29 ISBN 978-087969786-0

Contents Preface 11. Neurobiology Reveals How the Brain Works 1. Introduction PART III: HOW SHOULD WE PERCEIVE HUMANITY IN THE THIRD MILLENNIUM? PART I: HUMANITY IN A PRESCIENTIFIC UNIVERSE 12. The Blank Slate, the Human Nature, and the Biological Determinism Fallacies 2. Living on Automatic Pilot 13. Human Nature as Potentials for Forming 3. Between Gods and Beasts Communities 4. Our Negative Image of Our Animal Self 14. Science Enriches Our Appreciation of the Arts 5. Mind, Souls, Ideals, and the Ephemeral and Humanities PART II: CONFRONTING AND 15. Moral Values Bind a Community RECOGNIZING OUR BIOLOGY 16. The Human Condition and Our World View 6. Biology Becomes Mechanistic and Emerges as Change Every Generation a Science 17. Rethinking Science Teaching 7. The Human Body Is Composed of Cells 18. A Human Outlook for the Third Millennium 8. The Body Evolves Index 9. Most Human Traits Are Determined by Genes, Which Are Composed of DNA 10. We Have a Life Cycle and Sexuality That Is Genetically Programmed