Supplemental Table 4. Expression of CX3CR1 in specimens of ovarian cancer (multiple types).

Position Number Sex* Age* Organ* Pathology Diagnosis* Grade* Type* Intensity** Location Percentage*** Score****

2 100 200 A1 1 F 38 Ovary Krukenberg tumor - Malignant cytoplasmic

1 80 80 A2 2 F 37 Ovary Krukenberg tumor - Malignant cytoplasmic

100 100 A3 3 F 30 Ovary Krukenberg tumor - Malignant 1 cytoplasmic

0 n/a 0 0 A4 4 F 35 Ovary Krukenberg tumor - Malignant

0 0 0 0 A5 5 F 54 Ovary Krukenberg tumor - Malignant

1.5 100 150 A6 6 F 54 Ovary Krukenberg tumor - Malignant cytoplasmic

3 100 300 A7 7 F 36 Ovary Krukenberg tumor - Malignant cytoplasmic

1 50 50 A8 8 F 49 Ovary Krukenberg tumor - Malignant cytoplasmic

3 90 270 A9 9 F 35 Ovary Krukenberg tumor - Malignant cytoplasmic

0 0 0 0 A10 10 F 36 Ovary Krukenberg tumor - Malignant

1 10 10 B1 11 F 65 Ovary Carcinosarcoma - Malignant cytoplasmic

2 70 140 B2 12 F 15 Ovary Yolk sac tumor - Malignant cytoplasmic

3 80 240 B3 13 F 27 Ovary Yolk sac tumor - Malignant cytoplasmic 2 80 160 B4 14 F 20 Ovary Yolk sac tumor - Malignant cytoplasmic

2.5 80 200 B5 15 F 14 Ovary Yolk sac tumor - Malignant cytoplasmic

0.5 20 10 B6 16 F 32 Ovary Yolk sac tumor - Malignant cytoplasmic

1.5 80 120 B7 17 F 22 Ovary Yolk sac tumor - Malignant cytoplasmic

1 10 10 B8 18 F 16 Ovary Yolk sac tumor - Malignant cytoplasmic

2 100 200 B9 19 F 15 Ovary Yolk sac tumor - Malignant cytoplasmic

3 100 300 B10 20 F 36 Ovary Dysgerminoma - Malignant cytoplasmic

1.5 70 105 C1 21 F 35 Ovary Dysgerminoma - Malignant cytoplasmic

1 80 80 C2 22 F 41 Ovary Dysgerminoma - Malignant cytoplasmic

1 80 80 C3 23 F 12 Ovary Dysgerminoma - Malignant cytoplasmic

2 100 200 C4 24 F 19 Ovary Dysgerminoma - Malignant cytoplasmic

1.5 100 150 C5 25 F 11 Ovary Dysgerminoma - Malignant cytoplasmic

1.5 80 120 C6 26 F 25 Ovary Dysgerminoma - Malignant cytoplasmic

1 50 50 C7 27 F 25 Ovary Dysgerminoma - Malignant cytoplasmic

0.5 30 15 C8 28 F 28 Ovary Mature teratoma - Benign cytoplasmic

0 0 0 0 C9 29 F 18 Ovary Mature teratoma - Benign 2.5 100 250 C10 30 F 16 Ovary Mature teratoma - Benign cytoplasmic Teratoma with malignant transformation (squamous 3 100 300 D1 31 F 50 Ovary cell carcinoma) - Malignant perinuclear

3 100 300 D2 32 F 30 Ovary Mature teratoma - Benign cytoplasmic

3 100 300 D3 33 F 30 Ovary Mature teratoma - Benign cytoplasmic

D4 34 F 30 Ovary Mature teratoma - Benign not readable n/a n/a n/a

Mature teratoma (ovarian D5 35 F 38 Ovary tissue sparse) - Benign not readable n/a n/a n/a

3 100 300 D6 36 F 22 Ovary Mature teratoma - Benign cytoplasmic

3 100 300 D7 37 F 27 Ovary Mature teratoma - Benign cytoplasmic

D8 38 F 28 Ovary Mature teratoma - Benign not readable n/a n/a n/a

3 60 180 D9 39 F 24 Ovary Mature teratoma - Benign cytoplasmic

3 100 300 D10 40 F 33 Ovary Mature teratoma - Benign cytoplasmic

Mature teratoma (ovarian 2 100 200 E1 41 F 37 Ovary tissue) - Benign cytoplasmic

E2 42 F 39 Ovary Mature teratoma - Benign not readable n/a n/a n/a

E3 43 F 48 Ovary Mature teratoma - Benign not readable n/a n/a n/a

Mature teratoma (ovarian 1 100 100 E4 44 F 27 Ovary tissue) - Benign cytoplasmic

3 100 300 E5 45 F 54 Ovary Mature teratoma - Benign cytoplasmic E6 46 F 19 Ovary Mature teratoma (sparse) - Benign not readable n/a n/a n/a

2 100 200 E7 47 F 52 Ovary Mature teratoma - Benign cytoplasmic

Sertoli-stromal cell tumor 3 60 180 E8 48 F 26 Ovary (sparse) 3 Malignant cytoplasmic

1.5 70 105 E9 49 F 59 Ovary Sertoli-stromal cell tumor 2 Malignant cytoplasmic

3 100 300 E10 50 F 60 Ovary Granular cell tumor - Malignant cytoplasmic

2.5 80 200 F1 51 F 44 Ovary Granular cell tumor - Malignant cytoplasmic

1 70 70 F2 52 F 63 Ovary Granular cell tumor - Malignant cytoplasmic

1.5 70 105 F3 53 F 60 Ovary Granular cell tumor - Malignant cytoplasmic

3 90 270 F4 54 F 60 Ovary Granular cell tumor - Malignant cytoplasmic

1.5 50 75 F5 55 F 54 Ovary Granular cell tumor - Malignant cytoplasmic

2 90 180 F6 56 F 65 Ovary Granular cell tumor - Malignant cytoplasmic

0 0 0 F7 57 F 33 Ovary Granular cell tumor - Malignant n/a

1 40 40 F8 58 F 31 Ovary Granular cell tumor - Malignant cytoplasmic

1.5 40 60 F9 59 F 66 Ovary Granular cell tumor - Malignant cytoplasmic

2 100 200 F10 60 F 51 Ovary Granular cell tumor - Malignant cytoplasmic

1 70 70 G1 61 F 49 Ovary Granular cell tumor - Malignant cytoplasmic 1 30 30 G2 62 F 40 Ovary Granular cell tumor - Malignant cytoplasmic

2.5 100 250 G3 63 F 50 Ovary Granular cell tumor - Malignant cytoplasmic

0 0 0 G4 64 F 19 Ovary Granular cell tumor - Malignant n/a

100 150 G5 65 F 37 Ovary Thecoma - Malignant 1.5 cytoplasmic

2 100 200 G6 66 F 60 Ovary Thecoma - Malignant cytoplasmic

3 70 210 G7 67 F 37 Ovary Thecoma - Malignant cytoplasmic

2 100 200 G8 68 F 46 Ovary Thecoma - Malignant cytoplasmic

G9 69 F 56 Ovary Thecoma - Malignant not readable n/a n/a n/a

1 100 100 G10 70 F 71 Ovary Thecoma - Malignant cytoplasmic

1 100 100 H1 71 F 63 Ovary Thecoma - Malignant cytoplasmic

1.5 100 150 H2 72 F 66 Ovary Thecoma - Malignant cytoplasmic

1 15 15 H3 73 F 53 Ovary Thecoma - Malignant cytoplasmic

1 90 90 H4 74 F 20 Ovary Thecoma (sparse) - Malignant cytoplasmic

1 90 90 H5 75 F 15 Ovary Thecoma - Malignant cytoplasmic

1 100 100 H6 76 F 62 Ovary Thecoma - Malignant cytoplasmic

1.5 100 150 H7 77 F 45 Ovary Thecoma - Malignant cytoplasmic 1.5 80 120 H8 78 F 57 Ovary Thecoma - Malignant cytoplasmic

1.5 90 135 H9 79 F 66 Ovary Thecoma - Malignant cytoplasmic

1.5 70 105 H10 80 F 22 Ovary Gynandroblastoma - Malignant cytoplasmic

Simple cyst (fibrous I1 81 F 29 Ovary tissue) - Cyst not readable n/a n/a n/a

3 100 300 I2 82 F 35 Ovary Leuteivic cyst - Cyst cytoplasmic

I3 83 F 44 Ovary Cyst (ovarian tissue) - Cyst not readable n/a n/a n/a

I4 84 F 35 Ovary Cyst (fibrofatty tissue) - Cyst not readable n/a n/a n/a

I5 85 F 27 Ovary Cyst (fibrofatty tissue) - Cyst not readable n/a n/a n/a

I6 86 F 35 Ovary Simple cyst - Cyst not readable n/a n/a n/a

I7 87 F 42 Ovary Simple cyst (sparse) - Cyst not readable n/a n/a n/a

I8 88 F 37 Ovary Simple cyst (sparse) - Cyst not readable n/a n/a n/a

Cyst (fibrous tissue and I9 89 F 28 Ovary blood vessel) - Cyst not readable n/a n/a n/a

3 100 300 I10 90 F 27 Ovary Follicular cyst - Cyst cytoplasmic

Cancer adjacent normal J1 91 F 35 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a

Cancer adjacent normal J2 92 F 23 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a Cancer adjacent normal ovarian tissue (fibrous J3 93 F 36 Ovary tissue and blood vessel) - Normal no epithelium n/a n/a n/a Cancer adjacent normal ovarian tissue (lutein J4 94 F 48 Ovary cyst) - Normal no epithelium n/a n/a n/a

Cancer adjacent normal J5 95 F 51 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a

Cancer adjacent normal J6 96 F 40 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a

Cancer adjacent normal J7 97 F 24 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a

Cancer adjacent normal J8 98 F 39 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a

Cancer adjacent normal J9 99 F 39 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a

Cancer adjacent normal J10 100 F 46 Ovary ovarian tissue - Normal no epithelium n/a n/a n/a

* Information obtained from the manufacturer (US Biomax; Rockville, MD) as it pertains to the ovarian carcinoma metastasis tissue microarray OV1002; TMA slide contained singular cores of each case (total n=100)

** Intensity of staining was scored as negative (0), weak positive (1), moderate positive (2), and strong positive (3)

*** Denotes percentage of positive cells in the core

**** Score has been calculated as intensity multiplied by percentage of positive cells

***** In the specimens of normal ovary only ovarian surface epithelium was scored

* Information obtained from the manufacturer (US Biomax; Rockville, MD) as it pertains to the ovarian carcinoma metastasis tissue microarray OV1002; TMA slide contained singular cores of each case (total n=100)