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Karakterisasi Simplisia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Sintrong Skripsi Oleh: Putri Wulan Sari Nim 151501074

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Karakterisasi Simplisia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Sintrong Skripsi Oleh: Putri Wulan Sari Nim 151501074 KARAKTERISASI SIMPLISIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) METODE DPPH (1,1-diphenyl-2-picrylhidrazil) SKRIPSI OLEH: PUTRI WULAN SARI NIM 151501074 PROGRAM STUDI SARJANA FARMASI FAKULTAS FARMASI UNIVERSITAS SUMATERA UTARA MEDAN 2020 UNIVERSITAS SUMATERA UTARA KARAKTERISASI SIMPLISIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) METODE DPPH (1,1-diphenyl-2-picrylhidrazil) SKRIPSI Diajukan sebagai salah satu syarat untuk memperoleh gelar Sarjana Farmasi pada Fakultas Farmasi Universitas Sumatera Utara OLEH: PUTRI WULAN SARI NIM 151501074 PROGRAM STUDI SARJANA FARMASI FAKULTAS FARMASI UNIVERSITAS SUMATERA UTARA MEDAN 2020 UNIVERSITAS SUMATERA UTARA PENGESAHAN SKRIPSI KARAKTERISASI SIMPLISIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) METODE DPPH (1,1-diphenyl-2-picrylhidrazil) OLEH: PUTRI WULAN SARI NIM 151501074 Dipertahankan di Hadapan Penguji Skripsi Fakultas Farmasi Universitas Sumatera Utara pada Tanggal: 03 Maret 2020 Disetujui oleh: Panitia Penguji: Pembimbing, Dra. Suwarti Aris, M.Si., Apt Prof. Dr. Jansen Silalahi, M.App.Sc., Apt. NUP 990100000 NIP 195006071979031001 Dra. Suwarti Aris, M.Si., Apt Ketua Program Studi Sarjana Farmasi, NUP 990100000 Dr. Sumaiyah, M.Si., Apt. Dra. Erly Sitompul, M.Si., Apt. NIP 197712262008122002 NIP 1950066121980032001 Medan, 03 Maret 2020 Disahkan Oleh: Dekan Prof. Dr. Masfria, M.S., Apt. NIP 19570723198601200 UNIVERSITAS SUMATERA UTARA KATA PENGANTAR Puji syukur penulis ucapkan kepada Allah Swt yang telah memberikan karunia yang berlimpah sehingga penulis dapat menyelesaikan skripsi ini dengan judul “Karakterisasi Simplisia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Sintrong (Crassocephalum crepidioides (Benth.) S. Moore) Metode DPPH(1,1-Diphenyl-2-Picrylhidrazil)”.Skripsi ini diajukan sebagai salah satu syarat untuk memperoleh gelar Sarjana Farmasi di Fakultas Universitas Sumatera Utara. Pada kesempatan ini dengan segala kerendahan hati penulis mengucapkan terima kasih kepada Ibu Dr. Masfria, M.S., Apt., selaku pejabat Dekan Fakultas Farmasi USU Medan, telah menyediakan fasilitas kepada penulis selama perkuliahan di Fakultas Farmasi. Penulis juga mengucapkan terima kasih kepada Ibu Dra. Suwarti Aris, M.Si., Apt., yang telah membimbing dan memberikan petunjuk serta saran-saran selama penelitian hingga selesainya skripsi ini. Bapak Prof. Dr. Jansen Silalahi, M.App.,Sc., Apt., dan Ibu Dra. Erly Sitompul, M.Si., Apt., selaku dosen penguji yang telah memberikan kritik, saran dan arahan kepada penulis dalam menyempurnakan skripsi ini. Ibu Dr.Sumaiyah, M.Si., Apt., selaku penasehat akademik yang selalu memberikan bimbingan kepada penulis selama masa perkuliahan serta Bapak dan Ibu staf pengajar Fakultas Farmasi USU Medan yang telah mendidik selama perkuliahan. Penulis juga mempersembahkan rasa terima kasih yang tak terhingga kepada orangtua tercinta Bapak Mukijan dan Ibunda Suyatmi, kepada adik Dwi Dini Farah Diva dan Azka Nur Rizky, atas limpahan kasih sayang, doa dan dukungan yang tidak ternilai apapun. Penulis tak lupa mengucapkan terima kasih kepada sahabat Halima, Fitri, Fatma, Dita, Ianita dan teman-teman lainnya yang iv UNIVERSITAS SUMATERA UTARA tidak dapat disebutkan satu per satu yang telah banyak membantu selama penulisan skripsi ini. Penulis telah berusaha semaksimal mungkin untuk menyelesaikan penulisan skripsi ini, namun demikian penulis menyadari sepenuhnya bahwa skripsi ini masih jauh dari kesempurnaan, oleh karena itu penulis menerima kritik dan saran demi kesempurnaan skripsi ini. Penulis berharap semoga skripsi ini dapat memberikan manfaat bagi kita semua. Medan, Maret 2020 Penulis Putri Wulan Sari NIM 151501074 v UNIVERSITAS SUMATERA UTARA SURAT PERNYATAAN ORISINALITAS Saya yang bertanda tangan di bawah ini: Nama : Putri Wulan Sari Nomor Induk Mahasiswa : 151501074 Program Studi : Sarjana Farmasi Judul Skripsi : Karakterisasi Simplisia Dan Uji Aktivitas Antioksidan Ekstrak Etanol Daun Sintrong (Crassocephalum crepidioides (Benth.) S. Moore) Metode DPPH(1,1-Diphenyl-2-Picrylhidrazil) Dengan ini menyatakan bahwa skripsi yang saya buat adalah asli karya sendiri dan bukan plagiat. Apabila di kemudian hari diketahui skripsi saya tersebut terbukti plagiat karena kesalahan saya sendiri, maka saya bersedia diberi sanksi apapun oleh Program Studi Sarjana Farmasi Fakultas Farmasi Universitas Sumatera Utara. Saya tidak akan menuntut pihak manapun atas perbuatan saya tersebut. Demikian surat pernyataan ini saya perbuat dengan sebenarnya dan dalam keadaan sehat. Medan, Maret 2020 Putri Wulan Sari NIM 151501074 vi UNIVERSITAS SUMATERA UTARA KARAKTERISASI SIMPLISIA DAN UJI AKTIVITAS ANTIOKSIDAN EKSTRAK ETANOL DAUN SINTRONG (Crassocephalum crepidioides (Benth.) S. Moore) METODE DPPH (1,1-diphenyl-2-picrylhidrazil) ABSTRAK Latar Belakang: Tumbuhan sintrong merupakan herba yang tumbuh liar di kebun, tepi sungai, tanah lembab di wilayah tropis dan sub tropis. Tumbuhan ini mengandung senyawa flavonoid, saponin, tanin, glikosida, dan steroid/triterpenoid. Tujuan: Untuk mengetahui karakterisasi simplisia daun tumbuhan sintrong, skrining fitokimia serbuk simplisia dan ekstrak etanol daun tumbuhan sintrong, dan uji aktivitas antioksidan ekstrak etanol daun tumbuhan sintrong. Metode: Ekstraksi dilakukan secara perkolasi menggunakan pelarut etanol, pengujian aktivitas antioksidan ekstrak etanol daun tumbuhan sintrong dilakukan menggunakan metode DPPH (1,1-diphenyl-2-picryhydrazyl) sebagai pembanding Vitamin C dan diukur menggunakan alat spektrofotometer UV-Visibel. Hasil: Hasil karakterisasi simplisia daun tumbuhan sintrong diperoleh kadar air 7,98%; kadar sari larut dalam air 23,02%; kadar sari larut dalam etanol21,24%; kadar abu total 4,08%; dan kadar abu tidak larut asam 0,91%. Hasil skrining fitokimia serbuk simplisia dan ekstrak etanol daun tumbuhan sintrong memberikan hasil yang sama yaitu flavonoid, saponin, tanin, glikosida, dan steroid/triterpenoid. Hasil uji aktivitas antioksidan ekstrak etanol daun tumbuhan sintrong diperoleh nilai 5 sebesar 51,3792 mcg/ml dan Vitamin C diperoleh nilai IC50 sebesar 3,2179 mcg/ml. Kesimpulan: Karakterisasi simplisia daun tumbuhan sintrong memenuhi persyaratan mutu simplisia. Hasil skrining fitokimia yang terdapat pada serbuk simplisia dan ekstrak etanol daun tumbuhan sintrong memberikan hasil yang sama yaitu flavonoid, saponin, tanin, glikosida, dan steroid/triterpenoid. Ekstrak etanol daun tumbuhan sintrong memiliki aktivitas antioksidan kuat sedangkan Vitamin C memiliki aktivitas antioksidan sangat kuat. Kata Kunci: Tumbuhan sintrong, karakterisasi, antioksidan, DPPH vii UNIVERSITAS SUMATERA UTARA CHARACTERIZATION OF SIMPLICIA AND ANTIOXIDE ACTIVITY TESTS OF ETHANOL EXTRACT SINTRONG LEAF (Crassocephalum crepidioides (Benth.) S. Moore) DPPH METHOD (1,1-diphenyl-2-picrylhidrazil) ABSTRACT Background: Sintrong plants are herba that grow wild in gardens, river banks, moist soils in tropical and sub-tropical regions. This plant contains tannin compounds, glycosides, saponins, flavonoids, and steroids / triterpenoids. Objective: To determine the charactesrictics of simplex plants leaves of sintrong, phytochemical screening of simplicia powders and ethanol extracts of sintrong plant leaves, and antioxidant activity of ethanol extracts of sintrong plant leaves. Method: Extraction was carried out by percolation using ethanol solvent, antioxidant activity of ethanol extract of sintrong plant leaves was carried out using the DPPH (1,1-diphenyl-2-picryhydrazyl) method and comparred with Vitamin C, and measured using a UV-Visible spectrophotometer. Results: The results of the characterization of the simplex powder of the sintrong plant leaves obtained with moisture content of 7.98 %; water soluble extract content 23.02 %; soluble extract content in ethanol 21.24 %; total ash content of 4.08 %; and acid insoluble ash content of 0.91 %. The results of phytochemical simplicia screening and ethanol extract of sintrong plant leaves gave the same results, namely flavonoids, saponins, tannins, glycosides, and steroids/triterpenoids. The antioxidant activity test results of the ethanol extract of sintrong plant leaves obtained IC50 values of 51,3792 mcg/ml and Vitamin C obtained IC50 value is 3.2179 mcg/ml. Conclusion: Simplicia characterization of sintrong plant leaves meets the quality requirements of simplicia. The results of phytochemical screening found in simplicia powder and ethanol extract of sintrong plant leaves gave the same results, namely flavonoids, saponins, tannins, glycosides, and steroids/triterpenoids. The ethanol extract of the leaves of the sintrong plant has a strong antioxidant activity while Vitamin C has a very strong antioxidant activity. Keywords: Sintrong leaf, characterization, antioxidants, DPPH viii UNIVERSITAS SUMATERA UTARA DAFTAR ISI HALAMAN SAMPUL ......................................................................................... i HALAMAN JUDUL ............................................................................................. ii HALAMAN PENGESAHAN ............................................................................... iii KATA PENGANTAR .......................................................................................... iv HALAMAN PERNYATAAN ORISINALITAS .................................................. vi ABSTRAK ...........................................................................................................
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