Genetics: Published Articles Ahead of Print, published on October 18, 2007 as 10.1534/genetics.107.078717 The Formation of the Central Element of Synaptonemal Complex May Occur By Multiple Mechanisms: The Roles of the N- and C-Terminal Domains of the Drosophila C(3)G Protein in Mediating Synapsis and Recombination Jennifer K. Jeffress*,1, Scott L. Page*,2, Suzanne K. Royer†, Elizabeth D. Belden*, Justin Blumenstiel*, Lorinda K. Anderson†, and R. Scott Hawley*,‡ * Stowers Institute for Medical Research, Kansas City, Missouri 64110 † Department of Biology, Colorado State University, Fort Collins, Colorado 80523 ‡ Department of Physiology, University of Kansas School of Medicine, Kansas City, Kansas 66160 1 Present address: Institute of Molecular Biology, University of Oregon, Eugene, Oregon 97403 2 Present address: Comparative Genomics Centre, James Cook University, Townsville, Queensland 4811, Australia 1 Running Title: N- and C-Terminal Domains of C(3)G Keywords: Meiosis, Recombination, Synaptonemal Complex, Chromosome Corresponding Author: R. Scott Hawley Stowers Institute for Medical Research, 1000 E. 50th St., Kansas City, MO 64110. Phone: 816-926-4427 Fax: 816-926-2060 Email:
[email protected] 2 ABSTRACT In Drosophila melanogaster oocytes, the C(3)G protein comprises the transverse filaments (TFs) of the synaptonemal complex (SC). Like other TF proteins, such as Zip1p in yeast and SCP1 in mammals, C(3)G is comprised of a central coiled-coil-rich domain flanked by N- and C-terminal globular domains. Here, we analyze in-frame deletions within the N- and C-terminal regions of C(3)G in Drosophila oocytes. As is the case for Zip1p, a C-terminal deletion of C(3)G fails to attach to the lateral elements of the SC.