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BoothStop #1203 By More Imaging With Molecular Devices’ complete turnkey solutions for high-content screening, expect more imaging from your screening, more easily and affordably than ever before. More Imaging Systems ULTRA New ImageXpress ™ confocal high-content screening system INSTRUMENTS MICRO ImageXpress ™ high-content screening system ASSAYS ImageXpress® 5000A for live-cell and kinetic assays SOFTWARE INFORMATICS More Software DATA MANAGEMENT MetaXpress™ for uniform image acquisition, processing and analysis Application Modules for validated turnkey analysis of high- content images AcuityXpress™ database-driven cellular informatics software for efficient yet powerful data mining and statistical capabilities MetaMorph®: the gold standard for research microscopy More Assays Transfluor® high-content assays for GPCR activation Expect more. We’ll do our very best to exceed your expectations. now part of MDS Analytical Technologies tel. +1-800-635-5577 | www.moleculardevices.com www.roche-applied-science.com FuGENE® HD Transfection Reagent Measure the results of your transfection, not your transfection reagent. Are you confident that the cellular effects you observe are the result of your 2000 transfected plasmid? Or are your results due to differential gene expression caused ■ 1741 Genes affected by only this reagent by the transfection reagent you use? 1500 ■ Genes affected by Rely on FuGENE® HD Transfection Reagent to avoid the high levels of nonspecific, both reagents off-target effects that can be generated with other transfection reagents (Figure 1). 1000 1505 ■ Generate physiologically relevant data you can trust with a unique non- 500 liposomal formulation. 282 46 ■ Achieve greater cell survival 236 236 when transfecting with this low-cytotoxicity Number of differentially expressed genes 0 reagent that is sterile filtered and free of animal-derived components. L2K FuGENE® HD Transfection Reagent Switch to FuGENE® HD Transfection Reagent to obtain meaningful results today! For more information and a database of successfully transfected cell lines, or to Figure 1. Minimize off-target effects by using FuGENE® HD Transfection Reagent. FuGENE® HD purchase, please visit www.powerful-transfection.com Transfection Reagent or a reagent from another supplier To place your order by phone, call 800 262 1640. (L2K) was used to transfect MCF-7 cells (ATCC® HTB-22™). Subsequent microarray expression profiling Please join us Monday, December 3, from 6:15 - 8:15 p.m. in Convention experiments demonstrated that L2K significantly altered Center Room 201 for our Technology Workshop. See presentations of real- the expression levels of six times more genes than world applications where FuGENE® HD Transfection Reagent has enabled FuGENE® HD Transfection Reagent. (View the complete research advancements. Visit us in Booth 705, December 2 - 4, to discuss article online in Biochemica (2006) 4 at www.roche- exciting new products and technologies for your research applications. applied-science.com/publications/biochemica.htm) FuGENE is a registered trademark of Fugent, L.L.C., USA. Contact [email protected] for licensing and commercial applications. Roche Diagnostics The ATCC trademark and trade name and any and all ATCC catalog numbers are trademarks of the American Type Culture Collection. Roche Applied Science © 2007 Roche Diagnostics. All rights reserved. ASCB2007 Indianapolis, Indiana BoothStop #1203 By More MetaMorph The metamorphosis continues! Introducing MetaMorph® 7 imaging software from Molecular Devices. Combining the most flexible and powerful tools for image acquisition, processing, and analysis, MetaMorph 7 offers a complete solution for even the most demanding live-cell imaging needs. New in MetaMorph 7: 4-D Viewer/3-D Measurements: Visualize multi-dimensional data sets and obtain 3-D measurements Application Modules: Accelerate image analysis with biology- specific modules for automated cell segmentation and quantitation Scan Slide: Automatically acquire, tile and stitch images of large samples MetaMorph® Live Replay: Capture real-time events, including a user-defined buffer interval prior to each event Need to add hardware to the equation? Ask your local MetaMorph representative about our custom-integrated imaging workstations or visit www.metamorph.com for more information. Expect more. We’ll do our very best to exceed your expectations. now part of MDS Analytical Technologies tel. +1-800-635-5577 | www.moleculardevices.com INNOVATIVE DISCOVERY TOOLS FOR SIGNAL TRANSDUCTION RESEARCH The Highest Quality Antibodies for the Study of Translational Control ...from Cell Signaling Technology BiP (C50B12) Rabbit mAb #3177 Phospho-eIF2α (Ser51) (119A11) Rabbit mAb #3597 Confocal immunofluorescent analysis of Immunohistochemical analysis of paraffin-embedded human lung carcinoma (left) HeLa cells using #3177 (green). Actin and breast carcinoma (right) using #3597. filaments have been labeled with Alexa Fluor ® 555 phalloidin (red). Blue pseudocolor = DRAQ5 ™ (fluorescent DNA dye). eIF4G (C45A4) Rabbit mAb #2469 Confocal immunofluorescent analysis of HeLa cells using #2469 (green). Actin filaments have been labeled with Fluor ® 555 phalloidin (red). Blue = DRAQ5 ™ fluorescent DNA dye. 4E-BP1 (53H11) Rabbit mAb #9644 Confocal immunofluorescent analysis of HeLa cells using #9644 (green), note the nuclear localization. Actin filaments have been labeled with Alexa Fluor ® 555 phalloidin (red). Blue = DRAQ5 ™ fluorescent DNA dye. Alexa Fluor ® is a registered trademark of Molecular Probes, Inc. www.cellsignal.com Orders (toll-free) 1-877-616-CELL (2355) [email protected] Fax ordering 1-978-867-2488 Technical support (toll-free) 1-877-678-TECH (8324) [email protected] Inquiries [email protected] BoothStop #1203 By More Modes Molecular Devices is the #1 supplier of tunable microplate readers. Our two dual-monochromator multi-detection readers—the SpectraMax® M5e and FlexStation® 3 with integrated compound addition—give you superior results in all modes. Widest spectrum of absorbance, fluorescence, and luminescence applications in microplates or cuvettes FP performance that is unparalleled by any tunable instrument TRF, HTRF®, IMAP® TR-FRET, and other TR-FRET assay capabilities Integrated plate stacker option for SpectraMax M5e Direct reagent transfer with 8- or 16-channel pipettor option on FlexStation 3 to increase kinetic assay throughput Complete SpectraTest™ hardware and SoftMax® Pro GxP software validation tools Whether you’re screening at high throughput or developing assays in cuvettes or 6- to 384-well microplates, the SpectraMax M5e and FlexStation 3 can address a wider array of assays. e Molecular Devices’ multi-detection readers: SpectraMax M5 Expect more. We’ll do our very best to exceed your expectations. (top) and FlexStation 3 with integrated fluid transfer (bottom). now part of MDS Analytical Technologies tel. +1-800-635-5577 | www.moleculardevices.com More Assay Solutions GPCR Discover Molecular Devices’ novel, high-performance assay solutions for the four major drug targets—GPCRs, compound ion channels, kinases, and membrane transporters. ion transporter channel More GPCRs: Assays, cells, and expression vectors for screening all classes of GPCRs; Gi, Go, Gs, and Gq. FA Transfluor® cell-based high-content imaging assay G-protein compound FA FLIPR® Calcium, Calcium 3, Calcium 4 assays cAMP Ca2+ CatchPoint® cAMP & cGMP assays LiveWare® G-protein cells and plasmids kinase compound nucleus More Ion Channel Assays: Homogeneous no-wash assays for direct and indirect ion flux activity. ® P P P FLIPR Membrane Potential assay protein protein protein FLIPR® Potassium Ion Channel assay More Kinases, Phosphatases, and Phosphodiesterases: Proprietary IMAP® platform; non-radioactive and non-antibody-based assays for screening kinases, phosphatases, and phosphodiesterases. IMAP® kinase, phosphatase, and FP/TR-FRET assays IMAP® PDE, FP, and TR-FRET assays More Membrane Transporters: Homogeneous fluorescent-based assays for measuring transporter uptake activity in end-point and real-time kinetic modes. Neurotransmitter Transporter Uptake Assay Fatty Acid Uptake Assay now part of MDS Analytical Technologies tel. +1-800-635-5577 | www.moleculardevices.com © 2007 Thermo Fisher Scientific Inc. All rights reserved. rights Inc. All Scientific Thermo Fisher © 2007 subsidiaries. Inc. and its Scientific Thermo Fisher are the property of trademarks All Same people. Same support. New possibilities. If you work with proteins, you’ve relied on Pierce reagents. For gene silencing, you’ve depended on Dharmacon innovations. In cell culture, HyClone is likely a good friend. For PCR, ABgene has helped you along the way. And for high content screening, you’ve trusted Cellomics and BioImage. Visit us at Booth #419 Now these top names in life science are sold under the Thermo Scientific brand. You’ll still find the same technical support and application expertise that you’ve come to expect. Only now, we can develop new ways to integrate technologies to accelerate and improve your results even further. So yes, there’s a new brand name – Thermo Scientific. But you can keep your trusted relationships and high level of service as we unite to help you pursue new possibilities. Moving science forward Learn more at www.thermo.com/LSR. Part of Thermo Fisher Scientific Confocal STED White Light Laser TIRF Spinning Disk Imaging Unlocked! Innovation is the driving force at Leica Microsystems. Visit Leica at ASCB, booth #1102 to learn how Leica’s