Fermentation Yeast Extract – More Than Just a Nitrogen Source Yeast Extracts

EXCELLENCE IN YEAST

INNOVATIVE SOLUTIONS FOR BIOTECH APPLICATIONS

Fermentation Yeast Extract – More than just a nitrogen source Yeast Extracts – What makes them unique?

In the complex world of biotechnology and fermen- product. Yeast extract from brewers’ yeast is charac- tation, Leiber brewers’ yeast extracts have proven to terized by a high sustainability and thus the manu- be more than just a nitrogen source! Yeast extracts facturing can be regarded as ecologically valuable have a long tradition in the fermentation industry, and efficient. serving as a nutritious and effective complex nitrogen source for a broad spectrum of production microorganisms belonging to bacteria, fungi and yeasts. The production of beer, and thus the production of the by-product brewers’ yeasts is carried out mainly on the basis of renewable, natural raw materials such as barley, hops and water. The solids, including mainly the yeast biomass, remain in the tank as by-

Yeast extract composition and their benefits

Building blocks for biosynthesis

Important growth factors Supply of nitrogen

Metabolic driver Proteins & Nucleotides free amino acids Essential enzymatic cofactors for biocatalytic activities Vitamins & Minerals & Derivatives Pace maker of fermentation Traces Impacting and regulating intracellular transport processes Cell protection Carbohydrates & Derivatives Maintaining electrical potential via Storage compounds membranes Enzymatic cofactors

Cell protection against stress, osmotic pressure, free radicals

Yeast cell wall Brewers’ yeast cell wall consists of a substantially complex structure containing many interesting and functional components, such as β-1,3- and β-1,6-linked glucan, mannans, mannoproteins, sterols and phospholipids. All these components present a multitude of interesting functions, which serve as versatile tools among others in the field of wine production including clarification, improving fermentation performance and flavoring.

2 Brewers’ yeast extracts in biotech industry

Brewers’ yeast extracts from Leiber have found their metabolites production. The possible applications are way into countless applications, ranging from huge in industrial biotechnology, as well as in the beverage production and straight forward field of fermented food and beverages. It is the fermentations for manufacturing of starter cultures, perfect choice for applications from lab to big scale to complex processes of primary and secondary industrial fermentation.

Brewers’ yeast extract boosting vinegar production The following study shows the impact of brewers’ yeast extracts applied as nutrient for vinegar production.

[%] concentration [%] normalized normalized Productivity of acetic acid fermentation Acetic acid & ethanol

Day of fermentation [d] Day of fermentation [d]

Control: w/o yeast extract +10 g/L yeast extract +20 g/L yeast extract

Benefits of using brewers’ yeast extract (e.g. Leiber-Fermentation E) in a vinegar production: Increased acetic acid yield of up to 80%! Saving 40% of production time! Boosting production capacity! Cost savings! Significantly increased productivity!

Our in-house quality control system ensures top quality of our products. All Leiber fermentation products comply with the requirements of VLOG certification: A GMO free certification based on EGGenTDurchfG.

Fermentation Yeast Extracts

Leiber Fermentation S

Well-balanced brewers’ yeast extract

Applications: Bioethanol and biogas fermentation Brewing industry Yeast fermentation Cultivation of starter cultures for meat and dairy Organic acid production

Benefits: Partly debittered Contains a perfectly adjusted concentration of active hop oils Product code Product name Consistency Completely water soluble 100431 Leiber-Fermentation S Powder Light to medium brown powder 101099 Leiber-Fermentation S, V Paste Kosher & Halal 100394 Leiber-Fermentation S, L Liquid GMO free

Leiber Fermentation E

Pure and debittered brewers’ yeast extract

Applications: Production of amino and nucleic acids, organic acids, technical enzymes, unsaturated fatty acids Cultivation of starter cultures for meat and dairy Hormone and vaccine production

Benefits: Well-balanced concentration of free amino acids, growth promoting vitamins and trace elements Completely water soluble Light beige to light brown powder Product code Product name Consistency Kosher & Halal 100413 Leiber-Fermentation E Powder GMO free 101308 Leiber-Fermentation E, V Paste Gluten-free according to regulation (EU) 100393 Leiber-Fermentation E, L Liquid No 828/2014

Fermentation Yeast Extracts

Leiber Fermentation H

Purified and debittered brewers’ yeast extract Rich in amino acids

Applications: Vitamin production Hormone and vaccine production Production of amino and nucleic acids, organic acids, enzymes and other functional proteins

Benefits: High concentration of valuable free amino acids and proteins Complex source of nutrition, growth promoting Product code Product name Consistency vitamins and trace elements 100434 Leiber-Fermentation H Powder Completely water soluble 100395 Leiber-Fermentation H, L Liquid Light brown powder Kosher & Halal GMO free Gluten-free according to regulation (EU) No 828/2014

Leiber CeWaFerm®

Brewers’ yeast cell walls

Applications: Clarification of beverages, e.g. wine Reactivation of wine fermentation process

Benefits: Rich in beta-glucan, lipids & mannoproteins Absorb mycotoxins, sterols, polyphenols and proteins Light tan powder Kosher & Halal GMO free Product code Product name Consistency 101309 Leiber-CeWaFerm® Powder

5 Our biotechnological solutions

Process improvements Boosting growth of microorganisms in fermentation Enhancing product synthesis Reduction of fermentation time ⁄ increased profitability Improved productivity and capacity (e.g. acetic acid, ethanol, and more) Clarification of biological solutions, e.g. wine Reactivation of production cells during long lasting fermentation processes

Tailor made yeast extracts in view of: Product composition Grade of protein hydrolysation

Customized products due to your process and specific requirements

Fermentation Yeast Extracts

Leiber – Excellence in Yeast

For more than 60 years Leiber GmbH has been sup- plying high value brewers’ yeast products under the “Made in Germany” banner throughout the world. Their products are used in animal nutrition, food and biotech industry. Using a patented process for drying yeast, Leiber manufactures brewers’ yeast products and yeast extracts of the highest quality standard as its two factories in Germany, and further sites in Poland and Russia.

“The comprehensive know-how about the characteristics of our functional brewers’ yeast extracts combined with our experiences in biotech applications enables Leiber to act as ‘solution provider’ for their customers.”

Dr.-Ing. Guido Melzer, Global Business Development Manager Fermentation

Natural raw materials Modern patented technology Quality products “Made in Germany“ Leiber brewers’ yeast products are not genetically modified Individual scientific advice Leiber is ISO 22000:2005, FSSC 22000, IFS and VLOG certified Kosher & Halal Yeast extracts are common products made by extracting the cell contents (removing the cell walls). They are used as complex nutrients for culture media for fermentation applications.

Why yeast extract for fermentation?

Yeast extracts are essential nutrients for production organisms deliver nitrogen in form of proteins and amino acids deliver vitamins, minerals and traces as important cofactors and stimulants deliver nucleotides as important growth factors

Leiber GmbH Tel. +49 (0) 5461 9303-0 Hafenstraße 24 Fax +49 (0) 5461 9303-5033 49565 Bramsche www.leibergmbh.de Germany [email protected]