Yeast Extract

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Revision: 1 Effective Date: 9/5/2019 Technical Specification Sheet Specification Technical precipitate. Prepared Appearance (2% Solution): Appearance: Dehydrated Chemistry: and Physical Specifications Control Quality toSDS Refer Precaution culture cell and microbiological in carbon and media. acids, amino nitrogen, vitamins, provides Extract Yeast Procedure the of Principles applications. cell culture for recommended been have Extract Yeast containing media Several in mil studies bacterial for in media culture successful been has Extract Yeast drying. spray by a powder into dried and clear is filtered Extract Yeast resulting The step. a heating by solubl total is the Extract Yeast enzymes. selfi.e., autolysis, undergoes where it water, in resuspended and washed, yeast, baker’s 0.3% of concentration the in employed generally cultures cell and use bacteriological B occurring naturally preserve water is the Extract Yeast Explanation and Summary Product Extract Yeast setting. Yeast Extract Use Intended *% Moisture Filterability (100 ml, 5% solution) ml, Filterability (100 pH (2% Chloride: Sodium chloride): (excluding Ash 6.25) x (N Proteins Nitrogen: Amino Nitrogen: Total Dry Matter*: Physical/Chemistry

solution):

can becalculated using equationthe (

is an autolysate yeast of cells used media inin culture a microbiological preparing laboratory

Saccharomyces

is not intended for use in the diagnosis of disease or other conditions in humans. humans. in conditions other or disease of diagnosis in the use for intended is not

soluble portion of autolyzed yeast. The autolysis is carefully controlled to to controlled is carefully autolysis The yeast. autolyzed of portion soluble Powder is homogeneous, free homogeneous, is Powder

comp spp., in a in spp., carbohydrate

Yeast Extract

and is an excellent stimulator of bacterial growth. Yeast Extract is Extract Yeast growth. bacterial of stimulator excellent is an and lex vitamins. Yeast Extract is prepared and standardized for for standardized and is prepared Extract Yeast lex vitamins.

Prepared medium is brilliant to clear, amber, 100 e portion of this autolytic action. The autolytic activity is stopped is stopped activity autolytic The action. autolytic this of e portion % -

drymatter - Filterable through a 47 mm 0.2 micron filter 0.2 micron 47 mm a through Filterable

under 15 inches vacuum within 2 minutes vacuum inches 15 under 0.5%. Yeast Extract is typically prepa is typically Yeast Extract 0.5%.

%) = %) = % Moisture - rich plant medium. The yeast is harvested, yeast is harvested, The plant rich medium. ( NCM0218

- flowing, and light tan to beige. to light tan and flowing, 11.5 to 11.516.0% to 62.573.8% to 10.011.8% 4.5 to 5.8% 4.5 to 6.8 to 7.2 6.8 to

≥ 94.0%≥ ≤ 0.5%≤ )

digestion using the yeast’s the using digestion

k and other dairy products. anddairy other k

without or with a slight red by growing growing by red

Revision: 1 Effective Date: 9/5/2019 Technical Specification Sheet Specification Technical 7. 6. 5. 4. 3. 2. 1. References asdirected. stored to applies Expiry the originalcolor. from changed has appearance on contai stamped date toexpiration Refer Expiration closed. tightly container by keeping and light moisture from Protect storage temperature. the same at environment humidity low in a container 2 at media culture dehydrated Store Storage results. test for references toappropriate Refer Results Extract. Yeast using procedures specific for references toappropriate Refer Procedure Test Agar: Peptone on Properties Supporting Growth

Coagulase Positive Staphylococcus: Positive Coagulase coli: Escherichia 25g): (per Salmonella Count: Plate Standard Microbiology Staphylococcus aureus aureus Staphylococcus coli Escherichia recombinant protein production, in Vitro Cell Dev. Biol. Anim., 37:549 Anim., Biol. Dev. Cell Vitro in production, protein recombinant and growth cell insect for medium efficient of Design 2001. Agathose. Schneider, Bastin, Ikonomou, 178 59: Sons, Wiley & BioEngineering, Biotechnology system. vector expression baculovirus the using Chan, L., P. F. and Greenfield, S. Reid. 1998. Optimizing fed Stat United D. C. Washington, Association, Public Health Marshall, (ed.). R.T. food, of examination (eds.). Splittstoesser F. D. and C., Vanderzant, wastewater, water and of Clesceri S. L. D., A. Eaton, manualBAM/default.htm www.fda.gov/Food/ScienceResearch/LaboratoryMethods/BacteriologicalAnalytical

es Pharmacopeia Pharmacopeia es Microorganism

Inc.

2004. 2004.

ed. American Public Health Association, Washington, D.C. Association, Washington, Health Public American ed. .

, and A. E. Greenberg (eds.). (eds.). Greenberg E. A. and , Standard methods for the examination of dairy products, Standard of for the dairy methods examination products,

rd National Formulary 201 Formulary National

ed. American Public Health Association, Washington, D.C. Association, Washington, Health Public American ed.

30 °C

away from direct sunlight. direct sunlight. from away ner. ner. Product

2015

should be discarded if not free flowing, or if the the or if flowing, free not if discarded be should . Compendium of methods for the microbio the for of methods Compendium . 8.

2017. 2017. Expected Result Expected - ≤ 5000≤ cfu/g batch batch of production recombinant proteins product Negative Negative Negative G G Standard methods for the examination examination the for methods Standard Once opened and recapped, place place and recapped, opened Once rowth rowth - 559.

in its intact container when container intact its in

17 th

ed. American American ed.

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