DOCSLIB.ORG

Masterarbeit / Master's Thesis

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Masterarbeit / Master's Thesis MASTERARBEIT / MASTER’S THESIS Titel der Masterarbeit / Title of the Master‘s Thesis Description of two novel and as-yet uncultured endosymbionts of Acanthamoeba spp. verfasst von / submitted by Stefanie Michels BSc angestrebter akademischer Grad / in partial fulfilment of the requirements for the degree of Master of Science (MSc) Wien, 2016 / Vienna 2016 Studienkennzahl lt. Studienblatt / A 066 830 degree programme code as it appears on the student record sheet: Studienrichtung lt. Studienblatt / Masterstudium Molekulare Mikrobiologie, mikrobielle degree programme as it appears on Ökologie und Immunbiologie the student record sheet: Betreut von / Supervisor: Univ.-Prof. Dr. Matthias Horn 2 Table of content 1. Introduction ......................................................................................................................................... 7 1.1 Endosymbiosis and other definitions ............................................................................................. 7 1.2 Diversity of bacterial endosymbionts in free-living amoebae ........................................................ 8 1.3 General physiology of bacterial endosymbiosis ........................................................................... 11 1.3.1 The intracellular life-style ...................................................................................................... 11 1.3.2 Chlamydial metabolism and physiologic activity .................................................................. 12 1.4 Aim of the study ........................................................................................................................... 15 2. Material and Methods ........................................................................................................................ 17 2.1 Technical supply and computer software .................................................................................... 17 2.2 Consumables ................................................................................................................................ 18 2.3 Molecular biology kits .................................................................................................................. 18 2.4 Probes and primers ...................................................................................................................... 19 2.5 Growth media, buffers and other chemical solutions .................................................................. 20 2.6 Methods ....................................................................................................................................... 24 2.6.1 Isolation of amoebae of free-living amoebae using non-nutrient agar plates seeded with food bacteria ..................................................................................................................................... 24 2.6.2 Axenization of free-living amoebae ...................................................................................... 25 2.6.3 Fluorescence in-situ hybridization (FISH) .............................................................................. 25 2.6.4 16S rRNA gene sequencing of axenized amoeba cultures .................................................... 26 2.6.5 Cultivation of identified Acanthamoeba spp. HSC ................................................................ 31 2.6.6 Curing of Acanthamoeba spp. HSC ....................................................................................... 31 2.6.7 Defining the host range of endosymbionts ........................................................................... 32 2.6.8 Isolation of genomic DNA ...................................................................................................... 32 2.6.9 HeLa cell infection assay ....................................................................................................... 33 2.6.10 Growth comparison ............................................................................................................ 34 2.6.11 Infection cycles ................................................................................................................... 36 3 2.6.12 Extracellular infectivity Assay ............................................................................................. 37 2.6.13 Glucose Assay ..................................................................................................................... 38 3. Results ................................................................................................................................................ 41 3.1 Isolation of free-living amoebae using non-nutrient agar plates seeded with Gram-negative food bacteria and axenic cultivation .............................................................................................................. 41 3.2 Identification of novel endosymbionts of Acanthamoeba spp. ................................................... 42 3.2.1 Fluorescence in-situ hybridization results ............................................................................. 42 3.2.2 16S/18S rRNA gene sequencing results of amoeba cultures harboring endosymbionts ...... 43 3.3 Phylogenetic analysis ................................................................................................................... 45 3.4 Host range of the endosymbionts HSC3 and HSC8 ...................................................................... 47 3.4.1 Curing of the natural Acanthamoeba sp. HSC host ............................................................... 47 3.4.2 Infection of different Acanthamoeba spp., human and insect cell lines ............................... 48 3.5 Characterization of Endosymbiont of Acanthamoeba sp. HSC3 .................................................. 49 3.5.1 The endosymbiont HSC3 has a biphasic infection cycle ........................................................ 49 3.5.2 Uninfected Acanthamoeba sp. HSC show faster growth in comparison to acanthamoebae harbouring the endosymbiont HSC3 ................................................................................................. 51 3.5.3 Influence of the endosymbiont HSC3 on acanthamoeba viability ........................................ 53 3.5.4 Influence of nutrient availability on infectivity ..................................................................... 55 3.6 Endosymbiont of Acanthamoeba sp. HSC8 .................................................................................. 58 3.6.1 The infection cycle of endosymbiont HSC8 ........................................................................... 58 3.6.2 Uninfected Acanthamoeba sp. HSC show faster growth in comparison to acanthamoebae harbouring the endosymbiont HSC8 ................................................................................................. 61 3.6.3 Influence of the endosymbiont HSC8 on acanthamoeba viability ........................................ 63 3.6.4 Maintenance of infectivity during host-free incubation ....................................................... 65 4. Discussion ........................................................................................................................................... 69 4.1 Detection of endosymbionts of free-living amoebae .................................................................. 69 4.1.1 Isolation and axenization of free-living amoebae ................................................................. 69 4.1.2 Detection of endosymbionts within free-living amoebae using FISH ................................... 69 4.2 Identification and phylogenetic analysis of endosymbionts of Acanthamoeba sp. ..................... 70 4.3 Curing of natural Acanthamoeba spp. HSC host .......................................................................... 72 4.4 Host range of endosymbionts of Acanthamoeba spp. HSC3 and HSC8 ...................................... 73 4.4.1 HSC3 and HSC8 are able to infect a variety of Acanthamoeba spp. ..................................... 73 4 4.4.2 HSC3 and HSC8 are incapable to infect mammalian and insect cells .................................... 74 4.5 Characterization of the endosymbiont of Acanthamoeba sp. HSC3 ............................................ 76 4.5.1 The developmental cycle of the chlamydia HSC3 ................................................................. 76 4.5.2 Influence of the endosymbiont on growth and fitness of amoebae hosts ........................... 78 4.5.3 Host-free survival capability and maintenance of infectivity in relation to nutrient availability .......................................................................................................................................... 80 4.6 Characterization of the endosymbiont of Acanthamoeba sp. HSC8 ............................................ 82 4.6.1 The infection cycle of the endosymbiont HSC8 .................................................................... 82 4.6.2 Influence of the endosymbiont on growth and fitness of amoebae hosts ........................... 84 4.6.3 Host-free survival capacity and maintenance of infectivity .................................................. 86 5. Abstract .............................................................................................................................................. 89 6. Zusammenfassung .............................................................................................................................. 91 Appendix ...................................................................................................................................................
Load more

Recommended publications
  • Supplementary Information for Microbial Electrochemical Systems Outperform Fixed-Bed Biofilters for Cleaning-Up Urban Wastewater
    Electronic Supplementary Material (ESI) for Environmental Science: Water Research & Technology. This journal is © The Royal Society of Chemistry 2016 Supplementary information for Microbial Electrochemical Systems outperform fixed-bed biofilters for cleaning-up urban wastewater AUTHORS: Arantxa Aguirre-Sierraa, Tristano Bacchetti De Gregorisb, Antonio Berná, Juan José Salasc, Carlos Aragónc, Abraham Esteve-Núñezab* Fig.1S Total nitrogen (A), ammonia (B) and nitrate (C) influent and effluent average values of the coke and the gravel biofilters. Error bars represent 95% confidence interval. Fig. 2S Influent and effluent COD (A) and BOD5 (B) average values of the hybrid biofilter and the hybrid polarized biofilter. Error bars represent 95% confidence interval. Fig. 3S Redox potential measured in the coke and the gravel biofilters Fig. 4S Rarefaction curves calculated for each sample based on the OTU computations. Fig. 5S Correspondence analysis biplot of classes’ distribution from pyrosequencing analysis. Fig. 6S. Relative abundance of classes of the category ‘other’ at class level. Table 1S Influent pre-treated wastewater and effluents characteristics. Averages ± SD HRT (d) 4.0 3.4 1.7 0.8 0.5 Influent COD (mg L-1) 246 ± 114 330 ± 107 457 ± 92 318 ± 143 393 ± 101 -1 BOD5 (mg L ) 136 ± 86 235 ± 36 268 ± 81 176 ± 127 213 ± 112 TN (mg L-1) 45.0 ± 17.4 60.6 ± 7.5 57.7 ± 3.9 43.7 ± 16.5 54.8 ± 10.1 -1 NH4-N (mg L ) 32.7 ± 18.7 51.6 ± 6.5 49.0 ± 2.3 36.6 ± 15.9 47.0 ± 8.8 -1 NO3-N (mg L ) 2.3 ± 3.6 1.0 ± 1.6 0.8 ± 0.6 1.5 ± 2.0 0.9 ± 0.6 TP (mg
    [Show full text]
  • Taxonomy JN869023
    Species that differentiate periods of high vs. low species richness in unattached communities Species Taxonomy JN869023 Bacteria; Actinobacteria; Actinobacteria; Actinomycetales; ACK-M1 JN674641 Bacteria; Bacteroidetes; [Saprospirae]; [Saprospirales]; Chitinophagaceae; Sediminibacterium JN869030 Bacteria; Actinobacteria; Actinobacteria; Actinomycetales; ACK-M1 U51104 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae; Limnohabitans JN868812 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae JN391888 Bacteria; Planctomycetes; Planctomycetia; Planctomycetales; Planctomycetaceae; Planctomyces HM856408 Bacteria; Planctomycetes; Phycisphaerae; Phycisphaerales GQ347385 Bacteria; Verrucomicrobia; [Methylacidiphilae]; Methylacidiphilales; LD19 GU305856 Bacteria; Proteobacteria; Alphaproteobacteria; Rickettsiales; Pelagibacteraceae GQ340302 Bacteria; Actinobacteria; Actinobacteria; Actinomycetales JN869125 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae New.ReferenceOTU470 Bacteria; Cyanobacteria; ML635J-21 JN679119 Bacteria; Proteobacteria; Betaproteobacteria; Burkholderiales; Comamonadaceae HM141858 Bacteria; Acidobacteria; Holophagae; Holophagales; Holophagaceae; Geothrix FQ659340 Bacteria; Verrucomicrobia; [Pedosphaerae]; [Pedosphaerales]; auto67_4W AY133074 Bacteria; Elusimicrobia; Elusimicrobia; Elusimicrobiales FJ800541 Bacteria; Verrucomicrobia; [Pedosphaerae]; [Pedosphaerales]; R4-41B JQ346769 Bacteria; Acidobacteria; [Chloracidobacteria]; RB41; Ellin6075
    [Show full text]
  • Alternate Succession of Aggregate-Forming Cyanobacterial Genera Correlated with Their Attached Bacteria by Co-Pathways
    Science of the Total Environment 688 (2019) 867–879 Contents lists available at ScienceDirect Science of the Total Environment journal homepage: www.elsevier.com/locate/scitotenv Alternate succession of aggregate-forming cyanobacterial genera correlated with their attached bacteria by co-pathways Cong-Min Zhu a,Jun-YiZhangb,c,RuiGuanb, Lauren Hale d,NingChena,MingLie,Zu-HongLub, Qin-Yu Ge b, Yun-Feng Yang f, Ji-Zhong Zhou d,f,g,h,TingCheni,j,⁎ a MOE Key Laboratory of Bioinformatics, Bioinformatics Division, Center for Synthetic & Systems Biology, Beijing National Research Center for Information Science and Technology, Department of Automation, Tsinghua University, Beijing 100084, China b State Key Lab for Bioelectronics, School of Biological Science and Medical Engineering, Southeast University, Nanjing, China c Wuxi Environmental Monitoring Centre, Wuxi, China d Institute for Environmental Genomics, Department of Microbiology and Plant Biology, University of Oklahoma, Norman, OK, USA e College of Resources and Environment, Northwest A & F University, Yangling, People's Republic of China f State Key Joint Laboratory of Environment Simulation and Pollution Control, School of Environment, Tsinghua University, Beijing, China g School of Civil Engineering and Environmental Sciences, University of Oklahoma, Norman, OK, USA h Earth Sciences Division, Lawrence Berkeley National Laboratory, Berkeley, CA, USA i Institute for Artificial Intelligence, Department of Computer Science and Technology, Tsinghua University, Beijing 100084, China j Tsinghua-Fuzhou
    [Show full text]
  • The Human Gut Microbiota As a Reservoir for Antimicrobial Resistance Genes
    The human gut microbiota as a reservoir for antimicrobial resistance genes Elena Buelow The human microbiota as a reservoir for antimicrobial resistance genes PhD Thesis, University of Utrecht, The Netherlands ISBN/EAN: 978-94-6295-117-4 Cover art: Elena Buelow, Ivo Jelinek. Layout and design: Elena Buelow, Ivo Jelinek Printed by: Uitgeverij BOXPress | Proefschriftmaken.nl © Elena Buelow, Utrecht, The Netherlands. All rights reserved. No part of this thesis may be reproduced, stored in a retrieval system or transmitted by any means without permission of the author. The copyright of the articles that have been published or accepted for publication has been transferred to the respective journals. This work was supported by The Netherlands Organisation for Health Research and Development ZonMw (Priority Medicine Antimicrobial Resistance; grant 205100015) and by the European Union Seventh Framework Programme (FP7-HEALTH-2011-single-stage) ‘Evolution and Transfer of Antibiotic Resistance’ (EvoTAR) under grant agreement number 282004 The printing of this thesis was kindly supported by: University Medical Center Utrecht; Infection and Immunity Center Utrecht; and the Netherlands Society of Medical Microbiology (NVMM) and the Royal Netherlands Society for Microbiology (KNVM). The human gut microbiota as a reservoir for antimicrobial resistance genes De darm microbiota van de mens als een reservoir van antimicrobiële resistentiegenen (met een samenvatting in het Nederlands) Proefschrift ter verkrijging van de graad van doctor aan de Universiteit Utrecht op gezag van de rector magnificus, prof. dr. G.J. van der Zwaan, ingevolge het besluit van het college voor promoties in het openbaar te verdedigen op dinsdag 24 maart 2015 des middags te 12.45 uur door Elena Bülow geboren op 7 augustus 1982 te Kevelaer, Duitsland Promotor: Prof.
    [Show full text]
  • Ultraviolet Disinfection Impacts the Microbial Community Composition and Function of Treated Wastewater Effluent and the Receiving Urban River
    Ultraviolet disinfection impacts the microbial community composition and function of treated wastewater effluent and the receiving urban river Imrose Kauser 1 , Mark Ciesielski 1 , Rachel S Poretsky Corresp. 1 1 Department of Biological Sciences, University of Illinois at Chicago, Chicago, IL, United States Corresponding Author: Rachel S Poretsky Email address: [email protected] Background. In the United States, an estimated 14,748 wastewater treatment plants (WWTPs) provide wastewater collection, treatment, and disposal service to more than 230 million people. The quality of treated wastewater is often assessed by the presence or absence of fecal indicator bacteria. UV disinfection of wastewater is a common final treatment step used by many wastewater treatment plants in order to reduce fecal coliform bacteria and other pathogens; however, its potential impacts on the total effluent bacterial community are seemingly varied. This is especially important given that urban wastewater treatment plants (WWTPs) typically return treated effluent to coastal and riverine environments and thus are a major source of microorganisms, genes, and chemical compounds to these systems. Following rainfall, stormflow conditions can result in substantial increases to effluent flow into these systems. Methods. Here, we conducted a lab-scale UV disinfection on WWTP effluent using UV dosage of 100 mJ/cm2 and monitored the active microbiome in UV-treated effluent and untreated effluent over the course of 48h post-exposure using 16S rRNA sequencing. In addition, we simulated stormflow conditions with effluent UV-treated and untreated effluent additions to river water and compared the microbial communities to those in baseflow river water. We also tracked the functional profiles of genes involved in tetracycline resistance (tetW) and nitrification (amoA) in these microcosms using qPCR.
    [Show full text]
  • Taxonomic Hierarchy of the Phylum Proteobacteria and Korean Indigenous Novel Proteobacteria Species
    Journal of Species Research 8(2):197-214, 2019 Taxonomic hierarchy of the phylum Proteobacteria and Korean indigenous novel Proteobacteria species Chi Nam Seong1,*, Mi Sun Kim1, Joo Won Kang1 and Hee-Moon Park2 1Department of Biology, College of Life Science and Natural Resources, Sunchon National University, Suncheon 57922, Republic of Korea 2Department of Microbiology & Molecular Biology, College of Bioscience and Biotechnology, Chungnam National University, Daejeon 34134, Republic of Korea *Correspondent: [email protected] The taxonomic hierarchy of the phylum Proteobacteria was assessed, after which the isolation and classification state of Proteobacteria species with valid names for Korean indigenous isolates were studied. The hierarchical taxonomic system of the phylum Proteobacteria began in 1809 when the genus Polyangium was first reported and has been generally adopted from 2001 based on the road map of Bergey’s Manual of Systematic Bacteriology. Until February 2018, the phylum Proteobacteria consisted of eight classes, 44 orders, 120 families, and more than 1,000 genera. Proteobacteria species isolated from various environments in Korea have been reported since 1999, and 644 species have been approved as of February 2018. In this study, all novel Proteobacteria species from Korean environments were affiliated with four classes, 25 orders, 65 families, and 261 genera. A total of 304 species belonged to the class Alphaproteobacteria, 257 species to the class Gammaproteobacteria, 82 species to the class Betaproteobacteria, and one species to the class Epsilonproteobacteria. The predominant orders were Rhodobacterales, Sphingomonadales, Burkholderiales, Lysobacterales and Alteromonadales. The most diverse and greatest number of novel Proteobacteria species were isolated from marine environments. Proteobacteria species were isolated from the whole territory of Korea, with especially large numbers from the regions of Chungnam/Daejeon, Gyeonggi/Seoul/Incheon, and Jeonnam/Gwangju.
    [Show full text]
  • Abstract Tracing Hydrocarbon
    ABSTRACT TRACING HYDROCARBON CONTAMINATION THROUGH HYPERALKALINE ENVIRONMENTS IN THE CALUMET REGION OF SOUTHEASTERN CHICAGO Kathryn Quesnell, MS Department of Geology and Environmental Geosciences Northern Illinois University, 2016 Melissa Lenczewski, Director The Calumet region of Southeastern Chicago was once known for industrialization, which left pollution as its legacy. Disposal of slag and other industrial wastes occurred in nearby wetlands in attempt to create areas suitable for future development. The waste creates an unpredictable, heterogeneous geology and a unique hyperalkaline environment. Upgradient to the field site is a former coking facility, where coke, creosote, and coal weather openly on the ground. Hydrocarbons weather into characteristic polycyclic aromatic hydrocarbons (PAHs), which can be used to create a fingerprint and correlate them to their original parent compound. This investigation identified PAHs present in the nearby surface and groundwaters through use of gas chromatography/mass spectrometry (GC/MS), as well as investigated the relationship between the alkaline environment and the organic contamination. PAH ratio analysis suggests that the organic contamination is not mobile in the groundwater, and instead originated from the air. 16S rDNA profiling suggests that some microbial communities are influenced more by pH, and some are influenced more by the hydrocarbon pollution. BIOLOG Ecoplates revealed that most communities have the ability to metabolize ring structures similar to the shape of PAHs. Analysis with bioinformatics using PICRUSt demonstrates that each community has microbes thought to be capable of hydrocarbon utilization. The field site, as well as nearby areas, are targets for habitat remediation and recreational development. In order for these remediation efforts to be successful, it is vital to understand the geochemistry, weathering, microbiology, and distribution of known contaminants.
    [Show full text]
  • Biodiversity in the Era of Big Data. on the Problem of Taxonomy Assignment and the Distribution of Diversity in Complex Biological Systems
    University of Milan-Bicocca Department of Biotechnology and Biosciences Ph.D. in Biology – Cycle XXVI Biodiversity in the Era of Big Data. On the problem of taxonomy assignment and the distribution of diversity in complex biological systems. PhD thesis by: Anna Sandionigi Matr. N 745174 Project Supervisor: Maurizio Casiraghi PhD Contents 1 General introduction1 2 Section 15 2.0.1 Definition of Biodiversity . .5 2.0.2 Molecular biodiversity . .7 2.0.3 Contribution of the Next generation sequencing to the study of biodiversity . 11 2.0.4 Bioinformatics approach to taxonomy assignment . 13 2.0.5 Distribution analysis of of diversity . 18 3 Section 2 23 3.1 Introduction . 23 3.2 Material and Methods . 24 3.2.1 Samples description . 24 3.2.2 DNA extraction and amplification of coxI barcode . 26 3.2.3 Sequencing libraries preparation and 454 pyrose- quencing . 27 3.2.4 Bioinformatics pipeline for sequence analysis . 28 3.3 Result . 34 3.3.1 Sampling unit and sequencing yield . 34 3.3.2 Bioinformatics analysis pipeline results . 35 3.3.3 Denoising and chimera removal output . 35 3.3.4 Sigma (s) screening and identification of its best value . 36 3.3.5 Error rate evaluation . 40 3.3.6 Taxon assignment . 45 3.4 Discussion . 48 4 Section 3 55 i Contents 4.1 Introduction . 55 4.2 Materials and Methods . 58 4.2.1 Sampling . 58 4.2.2 DNA extraction . 60 4.2.3 16S rRNA amplification and pyrosequencing . 60 4.2.4 Sequences analysis . 61 4.2.5 Microbial Community Analyses .
    [Show full text]
  • Contents Topic 1. Introduction to Microbiology. the Subject and Tasks
    Contents Topic 1. Introduction to microbiology. The subject and tasks of microbiology. A short historical essay………………………………………………………………5 Topic 2. Systematics and nomenclature of microorganisms……………………. 10 Topic 3. General characteristics of prokaryotic cells. Gram’s method ………...45 Topic 4. Principles of health protection and safety rules in the microbiological laboratory. Design, equipment, and working regimen of a microbiological laboratory………………………………………………………………………….162 Topic 5. Physiology of bacteria, fungi, viruses, mycoplasmas, rickettsia……...185 TOPIC 1. INTRODUCTION TO MICROBIOLOGY. THE SUBJECT AND TASKS OF MICROBIOLOGY. A SHORT HISTORICAL ESSAY. Contents 1. Subject, tasks and achievements of modern microbiology. 2. The role of microorganisms in human life. 3. Differentiation of microbiology in the industry. 4. Communication of microbiology with other sciences. 5. Periods in the development of microbiology. 6. The contribution of domestic scientists in the development of microbiology. 7. The value of microbiology in the system of training veterinarians. 8. Methods of studying microorganisms. Microbiology is a science, which study most shallow living creatures - microorganisms. Before inventing of microscope humanity was in dark about their existence. But during the centuries people could make use of processes vital activity of microbes for its needs. They could prepare a koumiss, alcohol, wine, vinegar, bread, and other products. During many centuries the nature of fermentations remained incomprehensible. Microbiology learns morphology, physiology, genetics and microorganisms systematization, their ecology and the other life forms. Specific Classes of Microorganisms Algae Protozoa Fungi (yeasts and molds) Bacteria Rickettsiae Viruses Prions The Microorganisms are extraordinarily widely spread in nature. They literally ubiquitous forward us from birth to our death. Daily, hourly we eat up thousands and thousands of microbes together with air, water, food.
    [Show full text]
  • Lung Microbiome and Host Immune Tone in Subjects with Idiopathic Pulmonary Fibrosis Treated with Inhaled Interferon-Γ
    ORIGINAL ARTICLE INTERSTITIAL LUNG DISEASE Lung microbiome and host immune tone in subjects with idiopathic pulmonary fibrosis treated with inhaled interferon-γ Jing Wang 1,2, Melissa Lesko2, Michelle H. Badri3, Bianca C. Kapoor2, Benjamin G. Wu2, Yonghua Li2, Gerald C. Smaldone4, Richard Bonneau3,5,6, Zachary D. Kurtz7, Rany Condos2 and Leopoldo N. Segal2 Affiliations: 1Division of Pulmonary and Critical Care Medicine, Beijing Chaoyang Hospital, The Capital University of Medicine, Beijing, China. 2Division of Pulmonary, Critical Care and Sleep Medicine, New York University School of Medicine, New York, NY, USA. 3Dept of Biology, Center for Genomics and Systems Biology, New York University, New York, NY, USA. 4Division of Pulmonary, Critical Care and Sleep Medicine, State University of New York at Stony Brook, Stony Brook, NY, USA. 5Courant Institute of Mathematical Sciences, New York University, New York, NY, USA. 6Simons Center for Data Analysis, Simons Foundation, New York, NY, USA. 7Dept of Microbiology, New York University School of Medicine, New York, NY, USA. Correspondence: Leopoldo N. Segal, NYU School of Medicine, 462 First Avenue 7W54, New York, NY 10016, USA. E-mail: [email protected] ABSTRACT Therapies targeting inflammation reveal inconsistent results in idiopathic pulmonary fibrosis (IPF). Aerosolised interferon (IFN)-γ has been proposed as a novel therapy. Changes in the host airway microbiome are associated with the inflammatory milieu and may be associated with disease progression. Here, we evaluate whether treatment with aerosolised IFN-γ in IPF impacts either the lower airway microbiome or the host immune phenotype. Patients with IPF who enrolled in an aerosolised IFN-γ trial underwent bronchoscopy at baseline and after 6 months.
    [Show full text]
  • JPL Pub 12-12: Genetic Inventory, Final Report
    National Aeronautics and Space Administration Genetic Inventory Task Final Report Volume 1 of 2 Kasthuri Venkateswaran Myron T. La Duc Parag Vaishampayan OTHER SIGNIFICANT CONTRIBUTORS Shariff Osman Kelly Kwan Emilee Bargoma Alexander Probst Moogega Cooper James N. Benardini James A. Spry September 2012 National Aeronautics and Space Administration Jet Propulsion Laboratory California Institute of Technology Pasadena, California www.nasa.gov JPL Publication 12-12 09/12 JPL Publication 12-12 Genetic Inventory Task: Final Report Copyright This research was carried out at the Jet Propulsion Laboratory, California Institute of Technology, under a contract with the National Aeronautics and Space Administration. Reference herein to any specific commercial product, process, or service by trade name, trademark, manufacturer, or otherwise, does not constitute or imply its endorsement by the United States Government or the Jet Propulsion Laboratory, California Institute of Technology. © 2012 by the California Institute of Technology. U.S. Government sponsorship acknowledged. This report shall be referenced as follows. For Volume 1: Venkateswaran, K., La Duc, M. T., and Vaishampayan, P. (2012) Genetic Inventory Task: Final Report, Volume 1, JPL Publication 12-12. Jet Propulsion Laboratory, California Institute of Technology, Pasadena, CA. For Volume 2: Venkateswaran, K., La Duc, M. T., and Vaishampayan, P. (2012) Genetic Inventory Task: Final Report, Volume 2, JPL Publication 12-12. Jet Propulsion Laboratory, California Institute of Technology, Pasadena,
    [Show full text]
  • Appendix 1. New and Emended Taxa Described Since Publication of Volume One, Second Edition of the Systematics
    188 THE REVISED ROAD MAP TO THE MANUAL Appendix 1. New and emended taxa described since publication of Volume One, Second Edition of the Systematics Acrocarpospora corrugata (Williams and Sharples 1976) Tamura et Basonyms and synonyms1 al. 2000a, 1170VP Bacillus thermodenitrificans (ex Klaushofer and Hollaus 1970) Man- Actinocorallia aurantiaca (Lavrova and Preobrazhenskaya 1975) achini et al. 2000, 1336VP Zhang et al. 2001, 381VP Blastomonas ursincola (Yurkov et al. 1997) Hiraishi et al. 2000a, VP 1117VP Actinocorallia glomerata (Itoh et al. 1996) Zhang et al. 2001, 381 Actinocorallia libanotica (Meyer 1981) Zhang et al. 2001, 381VP Cellulophaga uliginosa (ZoBell and Upham 1944) Bowman 2000, VP 1867VP Actinocorallia longicatena (Itoh et al. 1996) Zhang et al. 2001, 381 Dehalospirillum Scholz-Muramatsu et al. 2002, 1915VP (Effective Actinomadura viridilutea (Agre and Guzeva 1975) Zhang et al. VP publication: Scholz-Muramatsu et al., 1995) 2001, 381 Dehalospirillum multivorans Scholz-Muramatsu et al. 2002, 1915VP Agreia pratensis (Behrendt et al. 2002) Schumann et al. 2003, VP (Effective publication: Scholz-Muramatsu et al., 1995) 2043 Desulfotomaculum auripigmentum Newman et al. 2000, 1415VP (Ef- Alcanivorax jadensis (Bruns and Berthe-Corti 1999) Ferna´ndez- VP fective publication: Newman et al., 1997) Martı´nez et al. 2003, 337 Enterococcus porcinusVP Teixeira et al. 2001 pro synon. Enterococcus Alistipes putredinis (Weinberg et al. 1937) Rautio et al. 2003b, VP villorum Vancanneyt et al. 2001b, 1742VP De Graef et al., 2003 1701 (Effective publication: Rautio et al., 2003a) Hongia koreensis Lee et al. 2000d, 197VP Anaerococcus hydrogenalis (Ezaki et al. 1990) Ezaki et al. 2001, VP Mycobacterium bovis subsp. caprae (Aranaz et al.
    [Show full text]