About Gempharmatech

About GemPharmatech

GemPharmatech Co., LTD. is a high-tech biotechnology enterprise specialized in the development of humanized mouse model and drug screening technology. "Innovation research institute of humanized model and drug screening technology " aimed at keeping establishing novel humanized animal models for medical research and drug development, improving the success rate of the drug screening for biological medicine industry and providing strong support and guarantee.

GemPharmatech is one of the first entities entitled “novel research and development institution” in Nanjing. GemPharmatech has developed a series of severe immune deficiency mouse strains with intellectual property rights, a full set of single and double target immune checkpoint humanized mouse on both C57BL/6 and BALB/c backgrounds.

With support of Jiangsu provincial institute of industry & technology and Jiangbei new area, based on the original resources and technologies of Nanjing university-Nanjing biomedical research institute, GemPharmatech will keep moving on with professional teams and human-oriented animal model innovation technology.

Three core technology platforms: ·Platform of generating humanized animal models; ·Drug screening service platform based on humanized animal models ·R&D platform of humanized antibody derived drugs.

History

National Resource AAALAC international certification Center for Mutant Mice Nanjing Biomedical Research Institute of Nanjing University IMPC Model Animal Establish Cas9 technology GemPharmatech Asian Mouse Mutagenesis National Laboratory Animal International Mouse Research Center platform Co.,Ltd Resource Association Center Phenotyping Consortium

2002 2006 2010 2011 2013 2017 Resource Technology Scale World Fourth of Mouse Disease Model World Third of Mouse Model Innovation 80000 IVC cages in 10000 m2 SPF Resources capability facility Unique member in China of International The world’s leading CRISPR/Cas9 >600 new strains developed per year Mouse Phenotyping Consortium (IMPC) technology platform China’s largest lab mouse supplier

Qualification

First and four consecutive times Export Animal Enterprise Laboratory animal production accredited by AAALAC in China Registration certificate and use certificate

The only national genetic engineering mouse center certificated by National Ministry of Science and Technology Utility Model Patents Approval by ICLAS

Quality With the strict quality control of veterinarians, GemPharmatech provides high-quality laboratory Control animals in line with international standards. The health check passes 41 items (containing 33 national standard items) including pathology, microbiology, parasitology, etc.

Genetic To prevent genetic contamination, genetic drift or genetic mutations, GemPharmatech uses SNP Control (Single Nucleotide Polymorphism) method to conduct genetic monitoring annually for background of inbred and genetically engineered mice. Dalmatian Mouse Action

Covering >20000 genes CKO/KO mice models resource center

Dalmatian Mouse Action（DMA） Phase I ： Over 1200 CKO strains,only $9900/strain Over 1800 KO strains,only $4900/strain As fast as 90 days

Hot gene spots selected covering research areas of tumor, metabolism,immune response, developmental biology, DNA and protein modification etc

Rich selection 3000 Strains available ,more strains will Lower price be added in the future. CKO only $9900/strain,KO only $4900/strain

Faster delivery More convinient Fastest delivery cycle is 90 days. All you have to do is online shopping.

www.gempharmatech.com/en Emice: [email protected]

- 2 - 2019 Resources and services Genetic engineering mouse service

With experienced CRISPR / Cas9, ZFN and TALEN gene targeting technologies, GemPharmatech has constructed many knock-out / knock-in models, with a wide range of applications. GemPharmatech has currently accomplished over 1000 gene modified rat and mouse models via CRISPR/Cas9, and has the capability to generate over 1000 models annually.

CRISPR/ Multiple Cas9 Targeting KO/CKO/KI

H11/Rosa26 Locus Transgenic One-stop service

KO first ES Cell KO/CKO/KI

Experienced Promising service Efficient & stable project techniques management

Professional R & D Advanced team equipments

Resources and services 2019 - 3 - CRISPR/Cas9 technology

The CRISPR/Cas9 system is a prokaryotic immune system that confers resistance to foreign genetic elements, such as plasmids and phages and provides a form of acquired immunity.The double strand complex, formed by crRNA and TracrRNA via base complementary, direct Cas9 protein to cleave target DNA sequences matching the crRNA. The HNH domain and RuvC-like domain of Cas9 protein will introduce DSB (Double strand break) at the target locus, DSB will be repaired by NHEJ(Non- homologous end joining) and random bases will be inserted or deleted. Frame- shift caused by the In-del mutation can destroy the target gene product.

China’s first single/ multiple gene Cas9 KO mouse [1] [2] [3] [4] [5] China’s first Cas9 CKO mouse China's first CKO rat model World’s first Cas9 KO dog World’s first Cas9 KO monkey

Advantages

Quality Cas9 KO projects with 100% success rate Guarantee Cas9 CKO/KI projects with 100% success rate

Zero off- Independent modifications on Cas9 system [6] target ZERO off-target reported

Longest flox region of 210 Kbp Flexible Largest deleted fragment of 23 Mbps Strategy 5 genes deleted simuntaneously Design Largest KI Fragment of 12 Kbps

Multiple Background C57BL/6JGpt, C57BL/6NGpt, NOD/LTJ, BABL/CGpt, etc. Choices

Price GemPharmatech TG/KO pricing Service Cost（USD） Turnaround Time （Months） CRISPR/Cas9 CKO & KI 17000 6-8 CRISPR/Cas9 point mutation 16000 6-8 Mouse Super-TG 14000 6-8 CRISPR/Cas9 KO 5500 5-6 Conventional ES targeting CKO/KI 27500 10-12 Transgenic 5000 3-4 Bac Transgenic 8000 3-5 Rat CRISPR/Cas9 KO 12000 6-8

- 4 - 2019 Resources and services CRISPR/Cas9 technology

■ Cas9 KO/CKO

▲CRISPR/Cas9 KO strategy diagram ▲CRISPR/Cas9 CKO strategy diagram

■ Cas9-KI/TM

▲ CRISPR/Cas9 KI strategy diagram ▲ CRISPR/Cas9 TM strategy diagram

■ Cas9-cTM

▲ CRISPR/Cas9 cTM strategy diagram

Resources and services 2019 - 5 - ES technology

ES targeting is a molecular biological technology based on homologous recombination of DNA and embryonic stem cell science. Homologous recombination takes place when exogeneous DNA fragment with high homology with host DNA sequence swaps with host DNA.

Advantages

Independent self-deletion Save 3 months at most technique

Modified Bac Targeting system Accomplished 300 Kbp fragment KI

Accomplished first CKO model of China in 2005 Professional teams >700 models successfully generated

Multiple background choices 129S6/SvEvTac，C57BL/6N，C57BL/6J

■ KO

▲Knock out strategy diagram

- 6 - 2019 Resources and services ES technology

■ CKO

▲CKO strategy diagram

■ KO first

▲KO first strategy diagram

■ KI

▲Knock in strategy diagram

Resources and services 2019 - 7 - Transgenic technology

Advantages High success rate, 100%

BAC transgenic technology to generate models with >300 Kbp fragment inserted

Multiple background available: C57BL/6JGpt, NOD/LTJ, FVB, BABL/CJ, C57BL/ 6NGpt, etc.

TG： TG

TG Vector Promoter Gene ORF pA

Release for injection

转基因

▲Schematic diagram of TG strategy ▲ H11/Rosa26 KI strategy diagram

The comparison between transgenic and Super-TG (H11/Rosa26 site insertion):

■ Transgenic Technology: ■ H11/Rosa26 site insertion:

Different expression levels Precise copy number Risk of random insertion Precise insertion site Expression not guaranteed Guaranteed expression Risk of affecting endogenous genes Avoid affecting endogenous genes Breeding/screening of lines needed Used directly Poor genetic stability High genetic stability Short Turnaround Time (<3 months) Long turnaround time (4-5 months)

Reference:

[1 ]Shen, B., et al. (2013). "Generation of gene-modified mice via Cas9/RNA-mediated gene targeting." Cell Res 23(5): 720-723. [2] Zhou, J., et al. (2014). "One-step generation of different immunodeficient mice with multiple gene modifications by CRISPR/Cas9 mediated genome engineering." Int J Biochem Cell Biol 46: 49-55. [3] Ma, Y., et al. (2014). "Generating rats with conditional alleles using CRISPR/Cas9." Cell Res 24(1): 122-125. [4] Zou, Q., et al. (2015). "Generation of gene-target dogs using CRISPR/Cas9 system." J Mol Cell Biol 7(6): 580-583. [5] Niu, Y., et al. (2014). "Generation of gene-modified cynomolgus monkey via Cas9/RNA-mediated gene targeting in one-cell embryos." Cell 156(4): 836-843. [6] Shen, B., et al. (2014). "Efficient genome modification by CRISPR-Cas9 nickase with minimal off-target effects." Nat Methods 11(4): 399-402.

- 8 - 2019 Resources and services Other mouse models

■ Table 1. Cre lines Research area Strain number Strain name Application

Broad spectrum expresssion emCin(CAG-Cre) Broad spectrum expression cre Cre T004055 B6-H11 /Gpt Nervous system expression em1Cin(Nes-Cre) Specific expression of the central and peripheral nervous system Cre T003763 B6-H11 /Gpt em1Cin(Tek-Cre) Endothelial cells expression Cre Endothelial expression Cre T003764 B6-H11 /Gpt em1Cin(icre) Hepatic specific expression Cre T003814 B6-Alb /Gpt em1Cin(cre/ERT2) Small intestine specific expression, homozygous death T003768 B6-Lgr5 /Gpt Others em1Cin(iCre) Myeloid cells expression Cre T003822 B6-Lyz2 /Gpt em1Cin(P2A-iCre) B cell specific expression Cre T003785 B6-Cd19 /Gpt ■ Table 2. Tool mice Research area Strain number Strain name Application FLPo recombinant enzyme whole-body expressed in high em1Cin(CAG-FLPo) FLP-FRT System T002183 B6-H11 /Gpt expression strain T003856 DBA/1-tdTomato/Gpt Fluorescent mouse em2Cd3198 T003654 B6-Csde1 /Gpt Fluorescent mouse em1Cin(CAG-LoxP-ZsGreen-Stop-LoxP-tdTomato) T006163 B6/JGpt-H11 /Gpt G T bifluorescence Others em26Cd52 em26Cd22 em1Cin(CAG-tdTomato) T003575 NOD-Prkdc Il2rg H11 /Gpt Immunodeficient fluorescent mouse em1Cd3174 T003303 B6-Csde1 /Gpt Fluorescent mouse After mating with cre mice, can express cas9 protein and red tm1(CAG-LSL-cas9,-tdTomato) T002249 B6-Gt(ROSA)26Sor /Gpt fluorescence

■ Table 3. Humanized model Research area Strain number Strain name Application

em1Cin T004363 BALB/cJGpt-hOX40 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) em1Cin(hCD28) T004279 C57BL/6- Pdcd1 Cd28 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) em1Cin(hTNFRSF9) T004082 C57BL/6-Pdcd1 Tnfrsf9 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) em1Cin(hBTLA) T004081 B6/JGpt-Pdcd1 Btla /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) tm1(hPDL1) T004025 BALB/c-Pdcd1 Pdl1 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) em1Cin(hTIGIT) T004023 BALB/c-Pdcd1 Tigit /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) tm1(hPDL1) T004022 B6-Pdcd1 Pdl1 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hLAG3) T003597 B6-Lag3 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hBTLA) T003585 C57BL/6-Btla /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1 E2E3) em1Cin(hTNFRSF4) T003532 B6-Pdcd1 Tnfrsf4 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hTIGIT) T003366 C57BL/6-Tigit /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hCTLA4) T003362 C57BL/6-Ctla4 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hCTLA4) Humanized model T003361 BALB/c-Ctla4 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hTNFRSF9) T003360 C57BL/6-Tnfrsf9 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hTNFRSF9) T003359 BALB/cJGpt- Tnfrsf9 /Gpt Humanized immune checkpoint, tumor immunotherapy tm1(hPDL1) T003260 BALB/c-Pdl1 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) T002726 BALB/c-Pdcd1 /Gpt Humanized immune checkpoint, tumor immunotherapy em2Cd947 T002476 B6/JGpt-CDH10 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hTNFRSF4 T002140 C57BL/6-Tnfrsf4 )/Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) T003095 C57BL/6-Pdcd1 /Gpt Humanized immune checkpoint, tumor immunotherapy tm1(hPDL1) T003455 C57BL/6-Pdl1 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hTIGIT) T003365 BALB/c-Tigit /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin em1Cin T004024 C57BL/6-huPDCD1 huTIGIT /Gpt Humanized immune checkpoint, tumor immunotherapy T001550 BALB/cJGpt-Tg(hCD3E BAC)102/Gpt Humanized immune checkpoint, tumor immunotherapy em1Cinh em1Cin T003804 BALB/c-hPDCD1 OX40 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) em1Cin(hCTLA4) T003826 B6/JGpt-Pdcd1 Ctla4 /Gpt Humanized immune checkpoint, tumor immunotherapy

Resources and services 2019 - 9 - Other mouse models

Research area Strain number Strain name Application

em1Cin(hPDCD1) em1Cin(hCTLA4) Humanized model T003720 BALB/c-Pdcd1 Ctla4 /Gpt Humanized immune checkpoint, tumor immunotherapy em1Cin(hPDCD1) em1Cin(hTNFRSF9) T004005 BALB/c-Pdcd1 Tnfrsf9 /Gpt Humanized immune checkpoint, tumor immunotherapy

■ Table 4. Immunodeficiency model Research area Strain number Strain name Application

nu D000521 BALB/c-Foxn1 /Gpt T cells deficiency em28Cd94 T001511 NOD-Rag1 /Gpt T,B cells deficiency em22Cd68 T001501 NOD-Rag1 /Gpt T,B cells deficiency em1Cd34 T001509 NOD-Rag2 /Gpt T,B cells deficiency em2Cd382 T001510 NOD-Rag2 /Gpt T,B cells deficiency em28Cd34 T001556 NOD-Prkdc /Gpt T,B cells deficiency em26Cd52 T001492 NOD-Prkdc /Gpt T,B cells deficiency em2Cd58 T003015 NOD-Prkdc /Gpt T,B cells deficiency em26 em26 T001475 NOD-Prkdc Il2rg /Gpt T,B cells deficiency em2Cd275in13 T001494 BALB/cJGpt-Prkdc /Gpt T,B cells deficiency em1Cd561 Immunodeficiency T001455 BALB/cJGpt-Prkdc /Gpt T,B cells deficiency hr em26Cd52 em26Cd22 N000285 B6;NOD-Hr Prkdc Il2rg /Gpt T,B,NK cells deficiency, Hairless em28Cd94 em26Cd22 T002289 NOD-Rag1 il2rg /Gpt T,B cells deficiency em21Cd34 T001499 NOD-Lyst /Gpt Abnormal leukocyte development em26Cin2 T001566 NOD-Lyst /Gpt Abnormal leukocyte development em31Cd23 T001497 NOD-Il2rg /Gpt NK cells deficiency em26Cd22 T001554 NOD-IL2rg /Gpt NK cells deficiency em9Cd161 T001620 NOD-Prf1 /Gpt Abnormal T cell development em13Cd130 T001181 NOD-Prf1 /Gpt Abnormal T cell development em22Cin8 T001562 NOD-H2Ab1 /Gpt T,B cells deficiency em21Cd4 T001557 NOD-B2M /Gpt T cells deficiency R28CC T001459 CBA/CaJGpt-Btk /Gpt B cells deficiency

■ Table 5. Conventional strain Research area Strain number Strain name Application

em2Cd479 T002407 BKS-Lepr /Gpt Diabetes ( Ⅱ ) em1Cd25 T001461 B6/JGpt-Lep /Gpt Diabetes ( Ⅱ ) Diabetes model T002040 C57BL/6JGpt DIO 60% Diabetes ( Ⅱ ) N000235 NOD/ShiLtJGpt Diabetes ( Ⅰ ) T001458 C57BL/6-Apoeem1Cd82/Gpt atherosclerosis Atherosclerosis model T001464 B6/JGpt-Ldlrem1Cd82/Gpt atherosclerosis MinC T001457 C57BL/6-Apc /Gpt Intestinal tumor Tumor model em1Cd T005332 B6/JGpt-p53 /Gpt Spontaneous tumor em3Cd4 Muscular dystrophy T003035 C57BL/10ScSnJGpt-Dmd /Gpt muscular dystrophy D000274 C57BL/6NGpt Common using inbred strains N000214 C57BLKS/JGpt Common using inbred strains N000023 C57BL/10JGpt Common using inbred strains N000018 A/JGpt Common using inbred strains N000017 129S1/SvImJGpt Common using inbred strains Inbred strains N000020 BALB/cJGpt Common using inbred strains -/- N000192 C57BL/10ScNJGpt Tlr4 N000219 DBA/1JGpt Common using inbred strains N000026 FVB/NJGpt Common using inbred strains N000013 C57BL/6JGpt Common using inbred strains N000016 DBA/2JGpt Common using inbred strains - 10 - 2019 Resources and services Other mouse models

■ Table 6. Others

Strain Strain Strain Strain Strain Strain Strain Strain Strain number name type number name type number name type

T001689 Aars2 Knock out,HR T004029 Atp6V0a2 Knock out,Cas 9 T004556 Cd151 Knock out,Cas 9 T004785 Abca6 Knock out,Cas 9 T003741 ATP6v0a4 Knock out,Cas 9 T003672 Cd226 Knock out,Cas 9 T003400 Abcb5 Knock out,Cas 9 T003384 Atp6v0c Knock out,Cas 9 T003591 CD28 Knock out,Cas 9 T003004 Abcg2 Knock out,Cas 9 T003385 ATP6v0d1 Knock out,Cas 9 T003154 CD40 Knock out,Cas 9 T001883 Abhd3 Knock out,Cas 9 T003541 ATP6v0d2 Knock out,Cas 9 T004437 CD47 Knock out,Cas 9 T002559 ABTB1 Knock out,Cas 9 T003546 ATP6V0E2 Knock out,Cas 9 T002181 Cdc42ep5 Knock out,HR T001884 Acads Knock out,Cas 9 T004116 ATP6V1F Knock out,Cas 9 T002476 CDH10 Knock out,Cas 9 T004908 Ackr3 Knock out,Cas 9 T004571 ATP6V1G1 Knock out,Cas 9 T003742 Cdkn2c Knock out,Cas 9 T001885 Acsm1 Knock out,Cas 9 T003547 ATP6V1G2 Knock out,Cas 9 T003411 Cep55 Knock out,Cas 9 T006602 Acta2 Knock out,Cas 9 T004078 ATP6V1H Knock out,Cas 9 T001440 Ces1f KO First,HR T003740 Acvr1c Knock out,Cas 9 T004282 ATP6VoE1 Knock out,Cas 9 T001983 Ces2e Knock out,Cas 9 T002380 Acvrl1 Knock out,Cas 9 T004436 Atpif1 Knock out,Cas 9 T001398 Ces2f KO First,HR T001723 Adarb1 Knock out,Cas 9 T004444 Aurka Knock out,Cas 9 T001984 Cfap45 Knock out,Cas 9 T001724 Adh4 Knock out,Cas 9 T004852 Aurkb Knock out,Cas 9 T003700 Cfi Knock out,Cas 9 T002450 Adipoq Knock out,Cas 9 T001892 AW112010 Knock out,Cas 9 T004438 Cftr Knock out,Cas 9 T004213 Adipor1 Knock out,Cas 9 T004909 Axl Knock out,Cas 9 T003215 cGAS Knock out,Cas 9 T002443 Adipsin Knock out,Cas 9 T001557 B2m Knock out,Cas 9 T002922 chaf1b Knock out,Cas 9 T004006 Adra1b Knock out,Cas 9 T001894 B3galt1 Knock out,Cas 9 T002178 Chchd5 Knock out,Cas 9 T003334 Adra2a CKO,Cas 9 T001725 Bap1 Knock out,Cas 9 T006211 Chd6 Knock out,Cas 9 T003402 Aff1 Knock out,Cas 9 T002487 Basonuclin 1 Knock out,Cas 9 T004900 Chek1 Knock out,Cas 9 T001886 Agap3 Knock out,Cas 9 T001895 Bbc3 Knock out,Cas 9 T005316 Chodl Knock out,Cas 9 T004281 AKAP12 Knock out,Cas 9 T001896 BC021785 Knock out,Cas 9 T003469 Chrm1 Knock out,Cas 9 T006174 Akap13 Knock out,Cas 9 T001897 BC026585 Knock out,Cas 9 T003568 Chrm3 Knock out,Cas 9 T001384 Akr1c12 KO First,ES T001386 BC048403 KO First,HR T002191 Chst12 Knock out,Cas 9 T001887 Akr1c21 Knock out,Cas 9 T001387 BC049715 KO First,HR T004398 Clcn6 Knock out,Cas 9 T003814 ALB-icre Knock in,Cas 9 T001899 Bcl2l13 Knock out,Cas 9 T004220 Cldn8 Knock out,Cas 9 T005310 Aldh1a1 Knock out,Cas 9 T001437 Bend3 KO First,HR T002449 Clec-2 Knock out,Cas 9 T001888 Aldh7a1 Knock out,Cas 9 T005077 Birc2 CKO,Cas 9 T004590 Clk1 Knock out,Cas 9 T001388 Alg1 KO First,ES T001679 Blvrb Knock out,HR T003524 Clk2 Knock out,Cas 9 T003676 Alkbh5 Knock out,Cas 9 T002723 BmprII Knock out,Cas 9 T004079 Clk3 Knock out,Cas 9 T001889 Angel2 Knock out,Cas 9 T001438 Bnip1 KO First,HR T004588 Clk4 Knock out,Cas 9 T002062 Ankle1 Knock out,HR T001401 Bod1 KO First,HR T002556 Cltc Knock out,Cas 9 T001685 Ankrd53 Knock out,HR T001790 Bola2 Knock out,HR T002871 Cnnm2 Knock out,Cas 9 T002049 ankrd65 Knock out,HR T001726 Brcc3 Knock out,Cas 9 T002065 Cnpy1 Knock out,HR T001478 Ap5z1 Knock out,HR T001439 Brix1 KO First,HR T001991 Cntn3 Knock out,Cas 9 T001890 Apbb3 Knock out,Cas 9 T001459 Btk Knock in,Cas 9 T004945 Col1a1 Knock out,Cas 9 T001457 Apc Knock in,Cas 9 T004114 C1orf115 Knock out,Cas 9 T002051 Corin Knock out,Cas 9 T003403 Arel1 Knock out,Cas 9 T002188 C1orf194 Knock out,Cas 9 T003097 Cotl1 Knock out,Cas 9 T001176 Ash1l KO First T001900 Car5b Knock out,Cas 9 T001992 Cox8a Knock out,Cas 9 T002451 ATF4 Knock out,Cas 9 T001172 Carm1 KO First,HR T001993 Cpa1 Knock out,Cas 9 T003132 Atg12 Knock out,Cas 9 T003126 Caspase1 Knock out,Cas 9 T001995 Cpa4 Knock out,Cas 9 T001746 Atg4a Knock out,Cas 9 T004691 Cbx1 Knock out,Cas 9 T005647 Cps1 Knock out,Cas 9 T005024 Atg4b CKO,Cas 9 T004840 Cbx3 Knock out,Cas 9 T001980 Creg1 Knock out,Cas 9 T001747 Atg4c Knock out,Cas 9 T001393 Ccdc124 KO First,HR T003569 Crif1 Knock out,Cas 9 T005026 Atg4d CKO,Cas 9 T001901 Ccdc129 Knock out,Cas 9 T001986 Crisp3 Knock out,Cas 9 T001891 Atp13a5 Knock out,Cas 9 T001981 Ccdc176 Knock out,Cas 9 T003084 Crispld2 Knock out,Cas 9 T006611 Atp2a2 Knock out,Cas 9 T001982 Ccdc83 Knock out,Cas 9 T004589 CRMP2 Knock out,Cas 9 T004039 Atp6v0a1 Knock out,Cas 9 T001922 Ccl2 Knock out,Cas 9 T003701 Crp Knock out,Cas 9 Resources and services 2019 - 11 - Other mouse models

Strain Strain Strain Strain Strain Strain Strain Strain Strain number name type number name type number name type

T003654 Csde1 Knock out,Cas 9 T002002 Esr2 Knock out,Cas 9 T002457 Fus Knock out,Cas 9 T004915 Csf3 Knock out,Cas 9 T002003 Etaa1 Knock out,Cas 9 T003232 Fut2 Knock out,Cas 9 T002080 Ctsm Knock out,HR T001445 Exosc4 KO First,HR T002980 Fxyd3 Knock out,Cas 9 T002064 Ctu2 Knock out,HR T001152 Ezh1 KO First,HR T002558 Fxyd6 Knock out,Cas 9 T002488 Cxcl9 Knock out,Cas 9 T002440 EZH2 Knock out,Cas 9 T002664 G3bp2 Knock out,Cas 9 T001749 Cyld Knock out,Cas 9 T002557 Fabp4 Knock out,Cas 9 T003040 Gas5 Knock out,Cas 9 T001175 Cyp17a1 KO First,HR T001688 Fam166b Knock out,HR T004951 Gata4 Knock out,Cas 9 T001394 Cyp3a41b KO First,HR T001951 Fam172a Knock out,Cas 9 T002007 Gcnt3 Knock out,Cas 9 T001988 Cyp3a59 Knock out,Cas 9 T005690 Fam210a Knock out,Cas 9 T001967 Gfra1 Knock out,Cas 9 T004568 Cyr61 Knock out,Cas 9 T002004 Fam213a Knock out,Cas 9 T002396 Gfra2 Knock out,Cas 9 T004778 Dach1 Knock out,Cas 9 T004801 Fam60a Knock out,Cas 9 T002754 Gitr Knock out,Cas 9 T002073 Dbil5 Knock out,HR T001187 Fancl KO First,HR T001941 GLT1 Knock out,Cas 9 T001728 Dctd Knock out,Cas 9 T003208 Fasl Knock out,Cas 9 T002009 Gm15800 Knock out,Cas 9 T001994 Dctpp1 Knock out,Cas 9 T002005 Fat4 Knock out,Cas 9 T004919 Gpbar1 Knock out,Cas 9 T003482 Dda1 Knock out,Cas 9 T001750 Fbxl12 Knock out,Cas 9 T009383 Gpc6 Knock out,Cas 9 T001996 Ddr2 Knock out,Cas 9 T001647 Fbxl15 Knock out,Cas 9 T004248 Gpd2 Knock out,Cas 9 T004693 Ddrgk1 Knock out,Cas 9 T001154 Fbxl18 KO First,HR T005321 Gpnmb Knock out,Cas 9 T004365 Ddt Knock out,Cas 9 T001229 Fbxl19 KO First,HR T004093 Gpr146 Knock out,Cas 9 T005007 Ddx24 Knock out,Cas 9 T001751 Fbxl2 Knock out,Cas 9 T002663 GPR151 Knock out,Cas 9 T001997 Ddx26b Knock out,Cas 9 T001674 Fbxl20 Knock out,Cas 9 T004877 Gpr18 Knock out,Cas 9 T003656 Ddx58 Knock out,Cas 9 T006915 Fbxl4 Knock out,Cas 9 T003678 Gpr43 Knock out,Cas 9 T001441 Defb43 KO First,HR T002063 Fbxl5 Knock out,Cas 9 T002420 Gprc6a Knock out,Cas 9 T004117 Dgat2 Knock out,Cas 9 T001754 Fbxl8 Knock out,Cas 9 T004782 Gpsm1 Knock out,Cas 9 T003231 DHCR7 Knock out,Cas 9 T001306 Fbxo10 KO First,HR T002992 Gsdma Knock out,Cas 9 Gsdma1; Gsdma2; T002685 DHHC5 Knock out,Cas 9 T001729 Fbxo15 Knock out,Cas 9 T002434 Gsdma3 CKO,Cas 9 T003052 Dkk1 Knock out,Cas 9 T001189 Fbxo16 KO First,HR T000459 Gsdma3 Knock out,HR T003079 Dkk3 Knock out,Cas 9 T000531 Fbxo2 KO First,HR T004133 Gsdmc1234 Knock out,Cas 9 T001998 Dlgap2 Knock out,Cas 9 T001218 Fbxo22 KO First,HR T002010 Gsdmd Knock out,Cas 9 T003771 Dlk2 Knock out,Cas 9 T001755 Fbxo24 Knock out,Cas 9 T004881 H19 Knock out,Cas 9 T002587 Dpep2 Knock out,Cas 9 T001756 Fbxo27 Knock out,Cas 9 T001562 H2Ab1 Knock out,Cas 9 T002076 Dpp6 Knock out,Cas 9 T001757 Fbxo28 Knock out,Cas 9 T003744 Havcr1 Knock out,Cas 9 T002000 Dtwd1 Knock out,Cas 9 T001228 Fbxo36 KO First,HR T000502 Hectd1 KO First,HR T002061 Dusp7 Knock out,HR T001758 Fbxo38 Knock out,Cas 9 T001206 Hectd3 Knock out T003086 E2f4 Knock out,Cas 9 T001675 Fbxo43 Knock out,Cas 9 T001762 Herc2 Knock out,Cas 9 T001442 Eddm3b KO First,HR T005040 Fbxo45 CKO,Cas 9 T001209 Herc4 KO First,HR T003404 Efhd2 Knock out,Cas 9 T005181 Fbxo6 Knock out,Cas 9 T000420 Herc6 KO First,HR T004526 Egfl6 Knock out,Cas 9 T001168 Fbxo8 KO First,HR T004439 Hfe2 Knock out,Cas 9 T003256 Ehf Knock out,Cas 9 T001760 Fbxw13 Knock out,Cas 9 T003147 Hfm1 Knock out,Cas 9 T001173 Ehmt1 KO First,HR T000438 Fbxw2 KO First,HR T006841 Hif1a Knock out,Cas 9 T004839 Eif5a Knock out,Cas 9 T000449 Fbxw8 KO First,HR T002192 Hilpda Knock out,Cas 9 T003743 Elavl2 Knock out,Cas 9 T002633 Fezf2 Knock out,Cas 9 T004234 Hmgcs2 Knock out,Cas 9 T004007 Elavl4 Knock out,Cas 9 T004665 Ffar1 Knock out,Cas 9 T003745 Hmox1 Knock out,Cas 9 T001684 Emc4 Knock out,HR T004996 Fhl1 Knock out,Cas 9 T002690 Hnrnpd Knock out,Cas 9 T001443 Emc7 KO First,HR T006218 Fis1 Knock out,Cas 9 T001763 Hnrnpdl Knock out,Cas 9 T004948 Emx2 Knock out,Cas 9 T003677 Flt3l Knock out,Cas 9 T000629 Hnrnpf KO First,HR T004718 Enkd1 Knock out,Cas 9 T002606 FMO2 Knock out,Cas 9 T001430 Hnrnph1 KO First,HR T006838 Entpd1 Knock out,Cas 9 T003167 Fnip1 Knock out,Cas 9 T001764 Hnrnpm Knock out,Cas 9 T002433 Erbb2ip Knock out,Cas 9 T003657 Fnip2 Knock out,Cas 9 T001804 Hnrnpr Knock out,Cas 9 T002001 Erich2 Knock out,Cas 9 T001179 Fntb KO First,HR T002011 Hoxc11 Knock out,Cas 9 - 12 - 2019 Resources and services Other mouse models

Strain Strain Strain Strain Strain Strain Strain Strain Strain number name type number name type number name type

T003041 Hoxd9 Knock out,Cas 9 T002017 Mast1 Knock out,Cas 9 T003209 Osgep Knock out,Cas 9 T004510 Htr1a Knock out,Cas 9 T003436 Mdr1b Knock out,Cas 9 T001813 Otud1 Knock out,Cas 9 T004922 Htr2b Knock out,Cas 9 T003042 Met Knock out,Cas 9 T001814 Otud3 Knock out,Cas 9 T001806 Htr4 Knock out,Cas 9 T003080 Mettl3 Knock out,Cas 9 T002595 Otud4 Knock out,Cas 9 T004134 Htr6 Knock out,Cas 9 T003748 Mga Knock out,Cas 9 T004681 Otud6A Knock out,Cas 9 T004278 Htra1 Knock out,Cas 9 T004368 Mgp Knock out,Cas 9 T001815 Oxsm Knock out,Cas 9 T002836 Icam1 Knock out,Cas 9 T003853 Mir122 Knock out,Cas 9 T004471 p16INK4a Knock out,Cas 9 T004080 ICOS Knock out,Cas 9 T005292 Mir29a Knock out,Cas 9,DKO T002837 p75NTR Knock out,Cas 9 T003168 Igfbp7 Knock out,Cas 9 T009392 Mir29a;Mir29b-1 Knock out,Cas 9,DKO T002550 Pacs2 Knock out,Cas 9 T004902 Il1a Knock out,Cas 9 T001545 Mir30a Knock out,Cas 9 T001217 Pan2 KO First,HR T003746 IL1b Knock out,Cas 9 T001165 Mir379 CKO,HR T006607 Panx1 Knock out,Cas 9 T005547 Il6 Knock out,Cas 9 T002947 Mir486 Knock out,Cas 9 T003194 Pard3 Knock out,Cas 9 T004367 ILF3 Knock out,Cas 9 T003680 Mir802 Knock out,Cas 9 T004280 Parp1 Knock out,Cas 9 T006789 Impact Knock out,Cas 9 T002470 Mlkl Knock out,Cas 9 T004690 Pate4 Knock out,Cas 9 T002012 Ins1 Knock out,Cas 9 T002348 Mlkl(S345D) Knock out,Cas 9 T004671 Pbld1/Pbld2 Knock out,Cas 9 T006228 Irx3 Knock out,Cas 9 T003133 Mpst Knock out,Cas 9 T003301 Pcdh20 Knock out,Cas 9 T003702 Itgb4 Knock out,Cas 9 T002806 Mpst Knock out,Cas 9 T004903 Peli1 Knock out,Cas 9 T004303 Jmjd3 Knock out,Cas 9 T002609 MS4a3 Knock out,Cas 9 T004906 Peli2 Knock out,Cas 9 T002031 Kcnh6 Knock out,Cas 9 T002157 Msln Knock out,Cas 9 T009091 Per2 Knock out,Cas 9 T003181 Kcnma1 Knock out,Cas 9 T004086 Muc5b Knock out,Cas 9 T005323 Pex13 Knock out,Cas 9 T003169 Kcnt1 Knock out,Cas 9 T003613 MyD88 Knock out,Cas 9 T001313 Pfkfb2 KO First,HR T001215 kdm1a Knock out,HR T003211 Myod1 Knock out,Cas 9 T002835 Pfkfb3 Knock out,Cas 9 T001807 Kdm2b Knock out,Cas 9 T004799 Ncor1 Knock out,Cas 9 T004020 PHB2 Knock out,Cas 9 T006581 Kdm3a Knock out,Cas 9 T003418 Neu1 Knock out,Cas 9 T001084 Phf1 Knock out,HR T001733 Kdm4a Knock out,Cas 9 T002020 Neu2 Knock out,Cas 9 T001210 Phf11 Knock out,HR T001808 Kdm5a Knock out,Cas 9 T003234 Neurog3 Knock out,Cas 9 T001292 Phf17 KO First,HR T002818 Kdm5b CKO,HR T009087 Nfia Knock out,Cas 9 T001734 Phf21b Knock out,Cas 9 T001809 Kdm5d Knock out,Cas 9 T003043 Nfib Knock out,Cas 9 T001735 Phf3 Knock out,Cas 9 T002817 Kdm7a Knock out,Cas 9 T009089 Nfkb1 Knock out,Cas 9 T001736 Phf5a Knock out,Cas 9 T004946 Kif2c Knock out,Cas 9 T003589 Nlrc5 Knock out,Cas 9 T001816 Phf8 Knock out,Cas 9 T001810 Kmt2a Knock out,Cas 9 T002108 Nmb Knock out,Cas 9 T004118 pick1 Knock out,Cas 9 T003286 Kmt2c Knock out,Cas 9 T004851 Nono Knock out,Cas 9 T004932 Pik3ip1 Knock out,Cas 9 T002755 Lag3 Knock out,Cas 9 T002578 Npepps Knock out,Cas 9 T003751 Pin1 Knock out,Cas 9 T003468 LAMP2 Knock out,Cas 9 T005644 Nppb Knock out,Cas 9 T006597 Pip5k1 Knock out,Cas 9 T002963 Laptm4b Knock out,Cas 9 T002750 NprA Knock out,Cas 9 T006177 Pip5k1c Knock out,Cas 9 T009101 Lbx1 Knock out,Cas 9 T009116 Nr1d1 Knock out,Cas 9 T003081 PNPLA3 Knock out,Cas 9 T009113 Lcat Knock out,Cas 9 T004924 Nr3c2 Knock out,Cas 9 T002452 Pom121 Knock out,Cas 9 T002438 LDB3 Knock out,Cas 9 T002917 Nr4a1 Knock out,Cas 9 T004527 Pou3f1 Knock out,Cas 9 T007096 Lgals1 Knock out,Cas 9 T003193 Nrf2 Knock out,Cas 9 T002453 Ppara Knock out,Cas 9 T004569 Lgi1 Knock out,Cas 9 T004779 Nrgn Knock out,Cas 9 T004528 Pparg Knock out,Cas 9 T002013 Lmo7 Knock out,Cas 9 T004377 Nrip1 Knock out,Cas 9 T005003 Ppm1b Knock out,Cas 9 T005687 Lrrc8a Knock out,Cas 9 T004221 Nrn1 Knock out,Cas 9 T000331 Ppm1j KO First,HR T003747 Lss Knock out,Cas 9 T004930 Nrxn3 Knock out,Cas 9 T003025 Ppp1cb Knock out,Cas 9 T004838 LTA4H Knock out,Cas 9 T002116 Nudc Knock out,Cas 9 T001153 Ppp1cc KO First,HR T002014 Lypd2 Knock out,Cas 9 T009122 Nudt1 Knock out,Cas 9 T001817 Ppp1r14a Knock out,Cas 9 T002015 Lypd5 Knock out,Cas 9 T009124 Nxph4 Knock out,Cas 9 T001314 Ppp1r14d KO First,HR T001499 Lyst Knock out,Cas 9 T009118 Ocln Knock out,Cas 9 T001818 Ppp1r1a Knock out,Cas 9 T002016 Maob Knock out,Cas 9 T002637 ODC1 Knock out,Cas 9 T001178 Ppp2r1b KO First,HR T003658 Mas1 Knock out,Cas 9 T003210 Oprm1 Knock out,Cas 9 T001740 Ppp2r2c Knock out,Cas 9 Resources and services 2019 - 13 - Other mouse models

Strain Strain Strain Strain Strain Strain Strain Strain Strain number name type number name type number name type

T000650 Ppp2r3a Knock out,HR T004512 Slc25a22 Knock out,Cas 9 T004370 Tspan1 Knock out,Cas 9 T000651 Ppp2r4 Knock out,HR T005655 Slc39a7 Knock out,Cas 9 T003170 uba5 Knock out,Cas 9 T001741 ppp2r5d Knock out,Cas 9 T004473 Slc4a11 Knock out,Cas 9 T005585 Ube2b Knock out,Cas 9 T001819 Ppp2r5e Knock out,Cas 9 T002069 slc6a4 Knock out,Cas 9 T000559 Ube2d3 KO First,HR T003217 Pqbp1 Knock out,Cas 9 T001937 Slco2a1 Knock out,Cas 9 T001846 Ube2e1 Knock out,Cas 9 T002118 Prdm2 Knock out,Cas 9 T002127 Slco6c1 Knock out,Cas 9 T005132 Ube2g2 CKO,Cas 9 T005011 Prdx3 Knock out,Cas 9 T004570 Slug Knock out,Cas 9 T000405 Ube2h KO First,HR T002119 Prex1 Knock out,Cas 9 T005645 Smad7 Knock out,Cas 9 T001847 Ube2k Knock out,Cas 9 T001620 prf1 Knock out,Cas 9 T004369 Smarca2 Knock out,Cas 9 T004802 Ube2o Knock out,Cas 9 T002120 Prickle4 Knock out,Cas 9 T002354 Smarcd2 Knock out,Cas 9 T001848 Ube2s Knock out,Cas 9 T003527 Prkaa1 Knock out,Cas 9 T004514 Smoothened Knock out,Cas 9 T001281 Ube2w KO First,HR T004008 Prkaa2 Knock out,Cas 9 T003406 SMP30 Knock out,Cas 9 T001870 Ube2z Knock out,Cas 9 T003405 Prmt5 Knock out,Cas 9 T001960 snrnp200 Knock out,Cas 9 T005205 Uchl1 Knock out,Cas 9 T002121 Prmt6 Knock out,Cas 9 T001400 Snx25 KO First,HR T001216 Uchl5 Knock out,HR T001208 Psmd14 Knock out,HR T002129 Sorcs2 Knock out,Cas 9 T005188 Ufc1 Knock out,Cas 9 T003271 Psmd4 Knock out,Cas 9 T002840 Sox2 Knock out,Cas 9 T004901 Ufl1 Knock out,Cas 9 T003470 Psme3 Knock out,Cas 9 T004453 Sp1 Knock out,Cas 9 T003171 ufm1 Knock out,Cas 9 T002961 Ptger4 Knock out,Cas 9 T002130 Spata16 Knock out,Cas 9 T003226 Ugcg Knock out,Cas 9 T003752 Ptpn2 Knock out,Cas 9 T000332 Speg KO First,HR T004440 Unc13c Knock out,Cas 9 T002286 Ptprv Knock out,Cas 9 T002932 Spp1 Knock out,Cas 9 T001207 Usp10 Knock out,HR T004529 Pura Knock out,Cas 9 T003085 Sptan1 Knock out,Cas 9 T001873 Usp11 Knock out,Cas 9 T004682 Pygm Knock out,Cas 9 T004937 Sptbn5 Knock out,Cas 9 T005058 Usp13 CKO,Cas 9 T006179 Rab1a Knock out,Cas 9 T006595 Sstr2 Knock out,Cas 9 T001874 Usp13 Knock out,Cas 9 T001184 Rabggta Knock out,HR T006596 Sstr3 Knock out,Cas 9 T001666 Usp15 Knock out,Cas 9 T004120 Rapgef4 Knock out,Cas 9 T005654 Sstr4 Knock out,Cas 9 T000618 Usp16 KO First,HR T002123 Rbm11 Knock out,Cas 9 T001947 Stk40 Knock out,Cas 9 T003172 Usp19 Knock out,Cas 9 T004716 Rc3h1 Knock out,Cas 9 T003408 Sucnr1 Knock out,Cas 9 T004836 Usp20 Knock out,Cas 9 T004087 Rdx Knock out,Cas 9 T003225 Sult2b1 Knock out,Cas 9 T002639 Usp22 Knock out,Cas 9 T009126 Reln Knock out,Cas 9 T001842 Suv39h1 Knock out,Cas 9 T005228 Usp24 Knock out,Cas 9 T004719 Rfx6 Knock out,Cas 9 T001843 Suv39h2 Knock out,Cas 9 T001875 Usp26 Knock out,Cas 9 T002124 Rgn Knock out,Cas 9 T006617 Suz12 Knock out,Cas 9 T001876 Usp30 Knock out,Cas 9 T002125 Rgs20 Knock out,Cas 9 T002439 SYVN1 Knock out,Cas 9 T001877 Usp31 Knock out,Cas 9 T002756 RhoA Knock out,Cas 9 T003854 Tcirg1 Knock out,Cas 9 T001920 Usp32 Knock out,Cas 9 T005692 Rnase10 Knock out,Cas 9 T004949 Tcp1 Knock out,Cas 9 T000439 Usp36 KO First,HR T004800 Ryr2 Knock out,Cas 9 T004009 Tdp2 Knock out,Cas 9 T001664 Usp37 Knock out,Cas 9 T003214 S1pr2 Knock out,Cas 9 T004788 Tead1 Knock out,Cas 9 T001085 Usp39 KO First,HR T003627 Samhd1 Knock out,Cas 9 T002651 TFRC Knock out,Cas 9 T001878 Usp40 Knock out,Cas 9 T003753 Scap Knock out,Cas 9 T003682 Tm6sf2 Knock out,Cas 9 T001213 Usp43 Knock out,Cas 9 T006843 Scarb2 Knock out,Cas 9 T003862 Tmem120a Knock out,Cas 9 T001879 Usp45 Knock out,Cas 9 T002801 scd1 Knock out,Cas 9 T002131 Tmem169 Knock out,Cas 9 T001880 Usp46 Knock out,Cas 9 T003595 Scn11a Knock out,Cas 9 T003681 Tmem175 Knock out,Cas 9 T001225 Usp47 KO First,HR T002682 Sec16b Knock out,Cas 9 T004241 Tmem63b Knock out,Cas 9 T001881 Usp49 Knock out,Cas 9 T001820 Senp2 Knock out,Cas 9 T002194 Tmigd1 Knock out,Cas 9 T001289 Usp50 Knock out,HR T001144 Senp7 KO First,HR T003754 Trem2 Knock out,Cas 9 T006918 Usp52 Knock out,Cas 9 T001822 Senp8 Knock out,Cas 9 T001844 Trim24 Knock out,Cas 9 T002356 Uspl1 Knock out,Cas 9 T009387 Sidt1 Knock out,Cas 9 T005509 Trim36 Knock out,Cas 9 T000827 UTX CKO,HR T001916 Sirpa Knock out,Cas 9 T001845 Trim37 Knock out,Cas 9 T002133 Vav2 Knock out,Cas 9 T002638 Skp2 Knock out,Cas 9 T003570 Trpv1 Knock out,Cas 9 T003306 Vegfr3(Flt4) Knock out,Cas 9 T009384 Slc25a12 Knock out,Cas 9 T000427 Tsg101 KO First,HR T004628 Virma Knock out,Cas 9

- 14 - 2019 Resources and services Other mouse models

Strain Strain Strain number name type

T003590 VISTA Knock out,Cas 9 T002146 Vps4b Knock out,Cas 9 T002135 Wfdc11 Knock out,Cas 9 T002136 Wwp1 Knock out,Cas 9 T003472 Wwtr1 Knock out,Cas 9 T004630 Xaf1 Knock out,Cas 9 T002824 Xbp1 Knock out,Cas 9 T004336 Xdh Knock out,Cas 9 T004511 Yap1 Knock out,Cas 9 T004115 YIPF6 Knock out,Cas 9 T003011 Ythdf2 Knock out,Cas 9 More information please T002629 ywhab Knock out,Cas 9 contact: T003037 ZDHHC16 Knock out,Cas 9 T001381 Zdhhc2 KO First,HR [email protected] T004812 Zfhx4 Knock out,Cas 9 T003722 Zfp422 Knock out,Cas 9 +86-025-58641520 T003283 Zfyve9 Knock out,Cas 9 T003296 zfyve9 Knock out,Cas 9 Web: www.gempharmatech.com T004688 Zmynd11 Knock out,Cas 9 www.nrcmm.cn T001446 Zswim1 KO First,HR

Resources and services 2019 - 15 - Publications

[1].Li, M., et al., APE1 deficiency promotes cellular senescence and premature aging features. Nucleic Acids Research, 2018. 46(11): p. 5664-5677.

[2].Xiong, X., et al., FNDC5 attenuates adipose tissue inflammation and insulin resistance via AMPK-mediated macrophage polarization in obesity. Metabolism, 2018. 83: p. 31-41.

[3].Yu, X., et al., Dynamics of chromatin marks and the role of JMJD3 during pancreatic endocrine cell fate commitment. Development, 2018. 145(6): p. dev163162.

[4].Shi, J., et al., MiR-31 Mediates Inflammatory Signaling to Promote Re-Epithelialization during Skin Wound Healing. Journal of Investigative Dermatology, 2018.

[5].Wang, L., et al., Intermedin Enlarges the Vascular Lumen by Inducing the Quiescent Endothelial Cell ProliferationHighlights. Arteriosclerosis, Thrombosis, and Vascular Biology, 2018. 38(2): p. 398-413.

[6].Ma, Q., et al., Mitochondrial PIP3-binding protein FUNDC2 supports platelet survival via AKT signaling pathway. Cell Death & Differentiation, 2018.

[7].Fu, T., et al., Mitophagy Directs Muscle-Adipose Crosstalk to Alleviate Dietary Obesity. Cell Reports, 2018. 23(5): p. 1357-1372.

[8].Hao, X., et al., SOX30 is a key regulator of desmosomal gene suppressing tumor growth and metastasis in lung adenocarcinoma. Journal of Experimental & Clinical Cancer Research, 2018. 37(1).

[9].Han, F., et al., SOX30 Inhibits Tumor Metastasis through Attenuating Wnt-Signaling via Transcriptional and Posttranslational Regulation of β-Catenin in Lung Cancer. EBioMedicine, 2018. 31: p. 253-266.

[10].Li, D., et al., Upregulation of Microglial ZEB1 Ameliorates Brain Damage after Acute Ischemic Stroke. Cell Reports, 2018. 22(13): p. 3574-3586.

[11].Liu, Y., et al., ICAM-1 Deficiency in the Bone Marrow Niche Impairs Quiescence and Repopulation of Hematopoietic Stem Cells.Stem Cell Reports, 2018. 11(1): p. 258-273.

[12].Chen, Q., et al., MicroRNA-23a/b and microRNA-27a/b suppress Apaf-1 protein and alleviate hypoxia-induced neuronal apoptosis. Cell Death & Disease, 2017. 5(3): p. e1132-e1132.

[13].Wang, L., et al., Deletion of Stk40 impairs definitive erythropoiesis in the mouse fetal liver. Cell Death & Disease, 2017. 8(3): p. e2722-e2722.

[14].Yang, Y., et al., Fusobacterium nucleatum Increases Proliferation of Colorectal Cancer Cells and Tumor Development in Mice by Activating Toll-Like Receptor 4 Signaling to Nuclear Factor−κB, and Up-regulating Expression of MicroRNA-21. Gastroenterology, 2017. 152(4): p. 851-866. e24.

[15].Gong, Y., et al., Tetratricopeptide repeat domain 3 overexpression tends to form aggregates and inhibit ubiquitination and degradation of DNA polymerase γ. Oncotarget, 2017. 8(63): p. 106475-106485.

[16].Huang, W., et al., Milk fat globule-EGF factor 8 suppresses the aberrant immune response of systemic lupus erythematosus-derived neutrophils and associated tissue damage. Cell Death & Differentiation, 2017. 24(2): p. 263-275.

[17].Lv, C., et al., MiR-31 promotes mammary stem cell expansion and breast tumorigenesis by suppressing Wnt signaling antagonists. Nature Communications, 2017. 8(1).

[18].Tian, Y., et al., Stress responsive miR-31 is a major modulator of mouse intestinal stem cells during regeneration and tumorigenesis. eLife, 2017. 6.

[19].Zhang, W., et al., Mitophagy receptor FUNDC1 regulates mitochondrial homeostasis and protects the heart from I/R iGptry. Autophagy, 2017. 13(6): p. 1080-1081.

- 16 - 2019 Resources and services [20].Qin, Y., et al., Targeted disruption of Noc4l leads to preimplantation embryonic lethality in mice. Protein & Cell, 2017. 8(3): p. 230-235.

[21].Yu, X., et al., Long non-coding RNA Linc-RAM enhances myogenic differentiation by interacting with MyoD. Nature Communications, 2017. 8: p. 14016.

[22].Dai, Y., et al., Conditional knockin of Dnmt3a R878H initiates acute myeloid leukemia with mTOR pathway involvement. Proceedings of the National Academy of Sciences, 2017. 114(20): p. 5237-5242.

[23].Sha, S., et al., Lack of JWA Enhances Neurogenesis and Long-Term Potentiation in Hippocampal Dentate Gyrus Leading to Spatial Cognitive Potentiation. Molecular Neurobiology, 2016. 53(1): p. 355-368.

[24].Zhang, Y., et al., miR-378 Activates the Pyruvate-PEP Futile Cycle and Enhances Lipolysis to Ameliorate Obesity in Mice. EBioMedicine, 2016. 5: p. 93-104.

[25].Zhang, W., et al., Hypoxic mitophagy regulates mitochondrial quality and platelet activation and determines severity of I/R heart iGptry. eLife, 2016. 5.

[26].Lyu, Y., et al., A Small Indel Mutant Mouse Model of Epidermolytic Palmoplantar Keratoderma and Its Application to Mutant-specific shRNA Therapy. Molecular Therapy - Nucleic Acids, 2016. 5: p. e299.

[27].Gordon, E.J., et al., The endothelial adaptor molecule TSAd is required for VEGF-induced angiogenic sprouting through junctional c-Src activation. Sci Signal, 2016. 9(437): p. ra72.

[28].Pei, L., et al., A Novel Mechanism of Spine Damages in Stroke via DAPK1 and Tau. Cerebral Cortex, 2015. 25(11): p. 4559-4571.

[29].Wu, J., et al., MicroRNA-30 family members regulate calcium/calcineurin signaling in podocytes. Journal of Clinical Investigation, 2015. 125(11): p. 4091-4106.

[30].Dong, Y., et al., Blocking follistatin-like 1 attenuates bleomycin-induced pulmonary fibrosis in mice. The Journal of Experimental Medicine, 2015. 212(2): p. 235-252.

[31].Liu, Z., et al., The histone H3 lysine-27 demethylase Jmjd3 plays a critical role in specific regulation of Th17 cell differentiation. Journal of Molecular Cell Biology, 2015. 7(6): p. 505-516.

[32].Zhang, F., et al., JMJD3 promotes chondrocyte proliferation and hypertrophy during endochondral bone formation in mice. Journal of Molecular Cell Biology, 2015. 7(1): p. 23-34.

[33].Yang, J., et al., Maged1 Co-interacting with CREB Through a Hexapeptide Repeat Domain Regulates Learning and Memory in Mice. Molecular Neurobiology, 2015. 51(1): p. 8-18.

[34].Ma, H., et al., HSPA12B: a novel facilitator of lung tumor growth. Oncotarget, 2015. 6(12): p. 9924.

[35].Lv, P., et al., Targeted disruption of Rab10 causes early embryonic lethality. Protein & Cell, 2015. 6(6): p. 463-467.

[36].Yan, G., et al., MicroRNA miR-302 inhibits the tumorigenicity of endometrial cancer cells by suppression of Cyclin D1 and CDK1. Cancer Letters, 2014. 345(1): p. 39-47.

[37].Ma, B., et al., Dapper1 promotes autophagy by enhancing the Beclin1-Vps34-Atg14L complex formation. Cell Research, 2014. 24(8): p. 912- 924.

[38].Long, L., et al., Recruitment of Phosphatase PP2A by RACK1 Adaptor Protein Deactivates Transcription Factor IRF3 and Limits Type I Interferon Signaling. Immunity, 2014. 40(4): p. 515-529.

[39].Su, X., et al., LRRC19 expressed in the kidney induces TRAF2/6-mediated signals to prevent infection by uropathogenic bacteria. Nature Communications, 2014. 5.

Resources and services 2019 - 17 -