Water Name of the Pilot Location: Vrbas River (Karanovic And

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Sector: Water Name of the Pilot Location: Vrbas River (Karanovic and Novoselija villages around Banja Luka, BiH, Republika Srpska) Proposed Project Title: Construction of sewers and waste water treatment for Karanovic and Novoselija, (protected water supply area for Banja Luka) Bosnia and Herzegovina (4000 p.e.) Preliminary cost: 4.1 million EUR Needs for technical assistance and investment: Technical assistance for pre-feasibility study and full project preparation. Investment costs Summary of existing Environmental Situation: Karanovic and Novoslija are two settlements located in the protection zone, because they are in a close distance to the intake of the drinking water for the water supply system of Banja Luka. There is no sewerage system and no waste water treatment plant for the settlements. At present households are supplied in septic tanks. Waste water is discharged directly into the river or into the ground. Strengths: Strong motivation to solve the problem, in order to reduce the health hazard linked to the pollution of the drinking water source. Weaknesses: There is not enough information gathered in order to assess the real risk and to identify the best solution. There is no feasibility study carried out. Opportunities: The projest has a good potential to be developed. Threats: The levels of tariffs might be too low to cover the costs. Project options: Sewerage system, and a) One centralised plant of 4,000 p.e., or b) two plants of 2,000 p.e. each. Summary of Directions for Future Work: To meet the requirements of the EU-Directives concerning Urban Waste Water Treatment (1991L0271-20/11/2003) and Drinking Water (1998L0083-3/11/1998) the following needs to be addressed: Gather of all necessary information for the inventory; Secure funds for the project preparation; Conduct feasibility study; Preliminary design of a WWTP and sewage system; Obtain all necessary permits; Secure funds for the investment; Definite design of the plant(s). 1.

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Knowl. Manag. Aquat. Ecosyst. 2021, 422, 13 Knowledge & © L. Raguž et al., Published by EDP Sciences 2021 Management of Aquatic https://doi.org/10.1051/kmae/2021011 Ecosystems Journal fully supported by Office www.kmae-journal.org français de la biodiversité RESEARCH PAPER First look into the evolutionary history, phylogeographic and population genetic structure of the Danube barbel in Croatia Lucija Raguž1,*, Ivana Buj1, Zoran Marčić1, Vatroslav Veble1, Lucija Ivić1, Davor Zanella1, Sven Horvatić1, Perica Mustafić1, Marko Ćaleta2 and Marija Sabolić3 1 Department of Biology, Faculty of Science, University of Zagreb, Rooseveltov trg 6, Zagreb 10000, Croatia 2 Faculty of Teacher Education, University of Zagreb, Savska cesta 77, Zagreb 10000, Croatia 3 Institute for Environment and Nature, Ministry of Economy and Sustainable Development, Radnička cesta 80, Zagreb 10000, Croatia Received: 19 November 2020 / Accepted: 17 February 2021 Abstract – The Danube barbel, Barbus balcanicus is small rheophilic freshwater fish, belonging to the genus Barbus which includes 23 species native to Europe. In Croatian watercourses, three members of the genus Barbus are found, B. balcanicus, B. barbus and B. plebejus, each occupying a specific ecological niche. This study examined cytochrome b (cyt b), a common genetic marker used to describe the structure and origin of fish populations to perform a phylogenetic reconstruction of the Danube barbel. Two methods of phylogenetic inference were used: maximum parsimony (MP) and maximum likelihood (ML), which yielded well supported trees of similar topology. The Median joining network (MJ) was generated and corroborated to show the divergence of three lineages of Barbus balcanicus on the Balkan Peninsula: Croatian, Serbian and Macedonian lineages that separated at the beginning of the Pleistocene.
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