Product datasheet Anti-ZNF318 Antibody Catalog Number: A11267-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Basic Information Product Name Anti-ZNF318 Antibody Gene Name ZNF318 Source Rabbit IgG Species Reactivity human, rat Tested Application WB, FCM, Direct ELISA Contents 500 ug/ml antibody with PBS ，0.02% NaN3 , 1 mg BSA and 50% glycerol. Immunogen E.coli-derived human ZNF318 recombinant protein (Position: E680-Q1197). Purification Immunogen affinity purified. Observed MW 290KD Dilution Ratios Western blot: 1:500-2000 Flow cytometry (FCM)： 1-3μg/1x106 cells Direct ELISA： 1:100-1000

Storage 12 months from date of receipt，-20℃ as supplied.6 months 2 to 8℃ after reconstitution. Avoid repeated freezing and thawing

Background Information Zinc finger protein 318 is a protein that in humans is encoded by the ZNF318 gene. ZNF318 encodes a nuclear protein with a zinc finger motif of the Cys2-His2 type that is a novel corepressor of androgen receptor (AR).

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FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 1 Product datasheet Anti-ZNF318 Antibody Catalog Number: A11267-1

BOSTER BIOLOGICAL TECHNOLOGY Special NO.1, International Enterprise Center, 2nd Guanshan Road, Wuhan, China

Web: www.boster.com.cn Phone: +86 27 67845390 Fax: +86 27 67845390 Email: [email protected]

Selected Validation Data

Figure 1. Western blot Figure 2. Flow cytometry analysis of anti- ZNF318 analysis of A431 cell Antibody (A11267-1). The (1x106) DyLight 488 sample well of each lane conjugated goat anti- was loaded with 50ug of rabbit IgG(blue) was used sample under reducing as secondary conditions. antibody.Isotype control Lane 1: THP-1 whole cell antibody (Green line) was lysates, rabbit IgG DyLight 488. Lane 2: rat stomach tissue Unlabelled sample (Red lysates, line). Lane 3: C6 whole cell lysates, Use rabbit anti- ZNF318 1:1000, probed with a goat anti-rabbit IgG-HRP secondary antibody. The signal is developed using an Enhanced Chemiluminescent detection (ECL) kit (Catalog # EK1002). A specific band was detected for ZNF318 at approximately 290KD. The expected band size for ZNF318 is at 251KD.

FOR RESEARCH USE ONLY. NOT FOR DIAGNOSTIC AND CLINICAL USE. 2

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