bioRxiv preprint doi: https://doi.org/10.1101/010009; this version posted October 3, 2014. The copyright holder for this preprint (which was not certified by peer review) is the author/funder, who has granted bioRxiv a license to display the preprint in perpetuity. It is made available under aCC-BY-NC-ND 4.0 International license. 1 2 Deep Sequencing revealed ‘Plant Like Transcripts’ in mosquito Anopheles culicifacies: an 3 Evolutionary Puzzle 4 5 Punita Sharma1,2, Swati Sharma1, Ashwani Kumar Mishra3, Tina Thomas1, Tanwee Das De1, Sonia Verma1, 6 Vandana Kumari1, Suman Lata Rohilla1, Namita Singh2, Kailash C Pandey1, Rajnikant Dixit1* 7 8 9 1Host-Parasite Interaction Biology Group, National Institute of Malaria Research, Sector-8, Dwarka, 10 Delhi-110077 (India); 2Nano and Biotechnology Department, Guru Jambheshwar University, Hisar, 11 Haryana-125001(India); 3NxGenBio Lifesciences, C-451, Yojna Vihar, Delhi-110092 (India) 12 13 14 For Correspondence: 15 16 *E.mail:
[email protected] 17 18 19 20 21 22 23 24 Abstract 25 26 As adult female mosquito’s salivary gland facilitate blood meal uptake and pathogen transmission e.g. 27 Plasmodium, virus etc., a plethora of research has been focused to understand the mosquito-vertebrate- 28 pathogen interactions. Despite the fact that mosquito spends longer time over nectar sugar source, the 29 fundamental question ‘how adult female salivary gland’ manages molecular and functional relationship 30 during sugar vs. blood meal uptake remains unanswered. Currently, we are trying to understand these 31 molecular relationships under dual feeding conditions in the salivary glands of the mosquito Anopheles 32 culicifacies.