DOCSLIB.ORG

Front Matter (PDF)

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Front Matter (PDF) AAI ANNUAL MEETING ANNUAL AAI PROGRAM PREVIEW ISSUE PREVIEW PROGRAM TH 97 Volume 184 l No. 6 l March 15, 2010 184 l No. 6 l March Volume www.jimmunol.org THE journal OF Immunology VOL. 184 | NO. 6 | March 15, 2010 | Pages 2741–3300 PROGRAM PREVIEW MIMMUNOLOGYMUNOLOG 2010YTM Important Links For program updates and complete meeting information, visit Online Registration www.IMMUNOLOGY2010.org/Registration.htm www.IMMUNOLOGY2010.org Hotel Reservations www.IMMUNOLOGY2010.org/Housing.htm International Attendees www.IMMUNOLOGY2010.org/Visa.htm THE AMERICAN ASSOCIATION OF IMMUNOLOGISTS | 97th ANNUAL MEETING May 7–11, 2010 | Baltimore Convention Center | Baltimore, Maryland | www.immunology2010.org IMMUNOLOGY 2010™ 97th Annual Meeting The American Association See you in Baltimore! of Immunologists All activities for the meeting will be held at the Baltimore May 7–11, 2010 N Baltimore, Maryland Baltimore Convention Center Convention Center (BCC) unless otherwise noted. www.immunology2010.org AAI PRESIDENT’S PROGRAM AAI President’s Address Presentation of AAI Lifetime FRIDAY, MAY 7 N 5:00 PM Achievement Award BCC BALLROOM I & II SATURDAY, MAY 8 N 5:00 PM N BCC BALLROOM I & II Betty A. Diamond, The Feinstein Institute for Chair: Betty A. Diamond, The Feinstein Institute Medical Research, AAI President for Medical Research, AAI President Antibodies and the brain: Award Recipient: Susan L. Swain lessons from lupus Trudeau Institute Matthew D. Scharff, Albert Einstein College The AAI Lifetime Achievement Award is the of Medicine, Introduction highest honor bestowed by the AAI Council upon an AAI member. This award recognizes Presentation of AAI Excellence a deserving member for a career of scientific in Mentoring Award achievement and for contributions to AAI and MONDAY, MAY 10 N 2:30 PM N BCC BALLROOM I & II fellow immunologists. The award will be presented prior to the start of Alan Sher’s AAI Distinguished Lecture. Chair: Betty A. Diamond, The Feinstein Institute for Medical Research, AAI President AAI Excellence in Mentoring Award AAI DISTINGUISHED LECTURES Award Recipient: Jack L. Strominger Generously supported by eBioscience, Inc. Harvard University Hidde Ploegh, MIT, Introduction SATURDAY, MAY 8 N 5:00 PM The AAI Excellence in Mentoring Award recognizes an BCC BALLROOM I & II AAI member’s exemplary career contributions to a future Alan Sher generation of scientists. The award will be presented prior NIAID, NIH to the start of the AAI President’s Symposium. Learning immunoregulation from parasites AAI President’s Symposium Neural and Immune Interactions: A New Frontier SUNDAY, MAY 9 N 5:00 PM MONDAY, MAY 10 N 2:30 PM–4:30 PM N BCC BALLROOM I & II BCC BALLROOM I & II Chair: Betty A. Diamond, The Feinstein Institute for Medical Research, AAI President Pamela S. Ohashi University of Toronto Kevin J. Tracey Britta Engelhardt Breaking T cell tolerance to tissue/tumor The Feinstein University of Bern antigens: a perspective nstitute for How immune cells Medical Research enter the central Reflex control of nervous system: MONDAY, MAY 10 N 5:00 PM mmunity anatomical pathways BCC BALLROOM I & II and molecular cues Mitchell Kronenberg La Jolla Institute for Allergy Madeleine W. Lawrence Steinman and Immunology Cunningham Stanford University Natural killer T cells ignite the immune University of Biomarkers guide Oklahoma Health patient selection for response to microbes by acting like Sciences Center argeting innate or innate immune cells Molecular mimicry in adaptive immunity in Dedicated to the memory of Eli Sercarz he immune system: multiple sclerosis effects on the brain IMMUNOLOGY 2010™ | THE AMERICAN ASSOCIATION OF IMMUNOLOGISTS AAI MAJOR SYMPOSIA SATURDAY, MAY 8 | 8:00 AM–11:30 AM SUNDAY, MAY 9 | 8:00 AM–11:30 AM BCC BALLROOM I BCC BALLROOM I Major Symposium A—Immunoimaging: Visualizing Major Symposium C—Regulation of Immunity by the Immune Response in Real-Time by Multiphoton Transcriptional and Epigenetic Networks Microscopy Chair: Kenneth M. Murphy, Washington University School Chair: Michael D. Cahalan, University of California, Irvine of Medicine Co-chair: Matthew F. Krummel, University of California, Co-chair: Dan R. Littman, New York University School San Francisco of Medicine UÊ Michael D. Cahalan, University of California, Irvine U Mark Schlissel, University of California, Berkeley There and back again: immunoimaging in peripheral tissue Transcription factors and signaling pathways that regulate and in lymphoid organs the V(D)J recombinase UÊ Matthew F. Krummel, University of California, San Francisco U Katia Georgopoulos, Massachusetts General Hospital/ Visualization of effective and defective antigen presentation Harvard Medical School, Ikaros and the rise and fall of the in vivo immune system UÊ Ronald N. Germain, NIAID/NIH, Connecting the dots: U Tian Chi, Yale University, Two novel genetic approaches for dynamic in situ analysis of innate and adaptive immunity studying epigenetic control in T cells using intravital 2-photon microscopy U Keji Zhao, NHLBI/NIH, Epigenetic regulation of T cell UÊ Ellen A. Robey, University of California, Berkeley, Imaging of differentiation T cells and thymocytes in action using 2-photon microscopy U Diane J. Mathis, Harvard Medical School, The molecular UÊ Mark J. Miller, Washington University School of Medicine mechanism of Aire Regulation of leukocyte trafficking in mouse models of U Kenneth M. Murphy, Washington University School of inflammation and infection Medicine, Immune lineage decisions regulated by atypical UÊ Thorsten R. Mempel, Massachusetts General Hospital AP-1 factors T cell migration and function during an anti-tumor immune response BCC BALLROOM II Major Symposium D—New Insights into Mast Cell BCC BALLROOM II Function Major Symposium B—Follicular Helper T Cells: Chair: Stephen J. Galli, Stanford University Lineage and Function Co-chair: Melissa A. Brown, Northwestern University Chair: Michael G. McHeyzer-Williams, Scripps Research U Juan Rivera, NIAMS/NIH, Sphingosine kinases and Institute sphingosine-1-phosphate in mast cell biology and function Co-chair: Carola G. Vinuesa, Australian National University U Toshiaki Kawakami, La Jolla Institute for Allergy and U Shane Crotty La Jolla Institute for Allergy and Immunology Immunology PLC-B3 a tumor suppressor inhibits skin AAI MAJOR SYMPOSIA MONDAY, MAY 10 | 8:00 AM–11:30 AM TUESDAY, MAY 11 | 8:00 AM–11:30 AM BCC BALLROOM I BCC BALLROOM I Major Symposium E—Multi-scale Computational and Major Symposium G—Evolution of Immunocompetent Systems Approaches to Understanding Immunity Lymphocytes and Their Receptors Chair: Ronald N. Germain, NIAID/NIH Chair: Max D. Cooper, Emory University Co-chair: Denise E. Kirschner, University of Michigan Co-chair: Martin F. Flajnik, University of Maryland at Baltimore Medical School UÊ Ellen Hsu, State University of New York, The basis of allelic UÊ Ronald N. Germain, NIAID/NIH, Introduction exclusion? UÊ Douglas A. Lauffenburger, MIT, Quantitative analysis of UÊ Martin F. Flajnik, University of Maryland at Baltimore interleukin-7 receptor/ligand dynamics, signaling, and Immunoglobulins and gamma/delta T cell receptors: responses in naive T cells: combined experiment and How do we decide? modeling studies U John P. Cannon, University of South Florida, Genetics, UÊ Martin Meier-Schellersheim, NIAID/NIH, Computational structure and function of novel immune receptors cell biology–from molecular interactions to cellular UÊ Max D. Cooper, Emory University, Evolution of alternative communication adaptive immune systems UÊ Byron Goldstein, Los Alamos National Laboratory U Thomas Boehm, Max-Planck-Institute of Immunobiology From ligand-induced receptor aggregation to the Evolution of the thymus in vertebrates aggregation of LAT: a systems biology approach to UÊ Roy A. Mariuzza, University of Maryland Biotechnology modeling FcERI-mediated mast cell signaling Institute, Structural insights into the evolution of adaptive U Penelope A. Morel, University of Pittsburgh, Under- immunity standing local innate immune responses to pathogens U Denise E. Kirschner, University of Michigan Medical BCC BALLROOM II School Multi-scale and multi-systems approaches toward Major Symposium H—Negative Regulatory Networks understanding the immune response to Mycobacterium in Health and Disease tuberculosis infection Chair: Arlene H. Sharpe, Harvard Medical School U Christophe Benoist, Harvard Medical School The immunological genome project Co-chair: Dario A. A. Vignali, St. Jude Children’s Research Hospital BCC BALLROOM II U Arlene H. Sharpe, Harvard Medical School, Role of co-inhibitory pathways in regulating the balance between Major Symposium F—Innate Immunoregulation at T cell activation and tolerance Mucosal Interfaces U David G. Brooks, UCLA, Positive and negative regulation Chair: Richard S. Blumberg, Brigham & Women’s Hospital/ of immunity during viral persistence Harvard Medical School U Dario A. A. Vignali, St. Jude Children’s Research Hospital Co-chair: Bana Jabri, University of Chicago Dissecting regulatory T cell function UÊ Thaddeus S. Stappenbeck, Washington University School U Christopher A. Hunter, University of Pennsylvania School of of Medicine, Autophagy, Paneth cells and intestinal inflam- Veterinary Medicine, Additional roles for IL-27 in immune mation regulation UÊ Richard S. Blumberg, Brigham & Women’s Hospital/ U Jane Hoyt Buckner, Benaroya Research Institute at Virginia Harvard Medical School, ER stress and intestinal inflammation Mason, Mechanisms of impaired tolerance in type 1 diabetes UÊ Andrew J.
Load more

Recommended publications
  • Development of VHH- and Antibody- Based
    Development of VHH- and Antibody- based Imaging and Diagnostic Tools by Zeyang Li Bachelor of Science in Biochemistry University of Wisconsin-Madison, WI, 2011 Submitted to the Department of Chemistry In Partial Fulfilment of the requirement for the Degree of Doctor of Philosophy at the Massachusetts Institute of Technology June 2018 2018 Massachusetts Institute of Technology All rights reserved Signature redacted Signature of Author........ Department of Chemistry 21 May 2018 Certified by.....................Signature redacted Hidde Ploegh OF TECHNOLOGY Senior Investigator Program in Cellular and Molecular Medicine JUN 2 0 Z018 At Boston Children's Hospital LIBRARIES ARCHIVES Signature redacted A cce pted by ....... .......................................... Robert W. Field Haslam and Dewey Professor of Chemistry Chairman, Departmental Committee for Graduate students This doctoral thesis has been examined by a committee of the Department of Chemistry as follows: ............................Signature redacted ----- Barbara Imperiali Class of 1922 Professor of Biology and Chemistry MacVicar Faculty Fellow Thesis Committee Chair Signature redacted Hidde Ploegh Senior Investigator Program in Cellular and Molecular Medicine Boston Children's Hospital / Z" Thes;Supervisor Signature redacted Bradley L. Pentelute Pfizer-Laubach Career Development Professor of Chemistry Abstract The immune system distinguishes self from non-self to combat pathogenic incursions. Evasion tactics deployed by viruses, microbes, or malignant cells may impede an adequate response. In such cases, therapeutic interventions aid in the elimination of pathogens and the restoration of physiological homeostasis. A major road block in the development of such therapies is the reliance on imperfect detection methods to identify site(s) of infection, or to monitor immune cell recruitment to sites of infection or inflammation in vivo.
    [Show full text]
  • Cell Biology from an Immune Perspective in This Lecture We Will
    Harvard-MIT Division of Health Sciences and Technology HST.176: Cellular and Molecular Immunology Course Director: Dr. Shiv Pillai Cell Biology from an Immune Perspective In this lecture we will very briefly review some aspects of cell biology which are required as background knowledge in order to understand how the immune system works. These will include: 1. A brief overview of protein trafficking 2. Signal transduction 3. The cell cycle Some of these issues will be treated in greater depth in later lectures. Protein Trafficking/The Secretory Pathway: From an immune perspective the secretory compartment and structures enclosed by vesicles are “seen” in different ways from proteins that reside in the cytosol or the nucleus. We will briefly review the secretory and endocytic pathways and discuss the biogenesis of membrane proteins. Some of the issues that will be discussed are summarized in Figures 1-3. Early endosomes Late endosomes Lysosomes Golgi Vesiculo-Tubular Compartment ER Figure 1. An overview of the secretory pathway Early endosomes Late endosomes Multivesicular and multilamellar bodies Golgi ER Proteasomes Figure 2. Protein degradation occurs mainly in lysosomes and proteasomes Proteins that enter the cell from the environment are primarily degraded in lysosomes. Most cytosolic and nuclear proteins are degraded in organelles called proteasomes. Intriguingly these two sites of degradation are each functionally linked to distinct antigen presentation pathways, different kinds of MHC molecules and the activation of different categories of T cells. Integral membrane proteins maybe inserted into the membrane in a number of ways, the two most common of these ways being considered in Figure 3.
    [Show full text]
  • Optimized Fluorescent Labeling to Identify Memory B Cells Specific for Neisseria Meningitidis Serogroup B Vaccine Antigens Ex Vivo
    Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo The MIT Faculty has made this article openly available. Please share how this access benefits you. Your story matters. Citation Nair, Nitya, Ludovico Buti, Elisa Faenzi, Francesca Buricchi, Sandra Nuti, Chiara Sammicheli, Simona Tavarini, et al. “ Optimized Fluorescent Labeling to Identify Memory B Cells Specific for Neisseria Meningitidis Serogroup B Vaccine Antigens Ex Vivo .” Immun. Inflamm. Dis. 1, no. 1 (October 2013): 3–13. As Published http://dx.doi.org/10.1002/iid3.3 Publisher Wiley Blackwell Version Final published version Citable link http://hdl.handle.net/1721.1/92523 Terms of Use Creative Commons Attribution Detailed Terms http://creativecommons.org/licenses/by/3.0/ ORIGINAL RESEARCH Optimized fluorescent labeling to identify memory B cells specific for Neisseria meningitidis serogroup B vaccine antigens ex vivo Nitya Nair1†, Ludovico Buti1,2‡, Elisa Faenzi1, Francesca Buricchi1, Sandra Nuti1, Chiara Sammicheli1, Simona Tavarini1, Maximilian W.L. Popp2,3,4, Hidde Ploegh2,4, Francesco Berti1, Mariagrazia Pizza1, Flora Castellino1, Oretta Finco1, Rino Rappuoli1, Giuseppe Del Giudice1, Grazia Galli1, & Monia Bardelli1 1Novartis Vaccines & Diagnostics, Siena, Italy 2Whitehead Institute for Biomedical Research, Cambridge, Massachusetts 3University of Rochester, School of Medicine & Dentistry, Rochester, New York 4Department of Biology, Massachusetts Institute of Technology, Cambridge, Massachusetts Keywords Abstract Antigen-specific memory B cells, flow cytometry, Neisseria meningitidis MenB, Antigen-specific memory B cells generate anamnestic responses and high affinity sortagging, vaccination antibodies upon re-exposure to pathogens. Attempts to isolate rare antigen- specific memory B cells for in-depth functional analysis at the single-cell level have Correspondence been hindered by the lack of tools with adequate sensitivity.
    [Show full text]
  • Contents Research Illuminating the Interplay of Cancer and the Immune System July 2013 Volume 1 Issue 1
    Cancer Immunology Contents Research Illuminating the Interplay of Cancer and the Immune System July 2013 Volume 1 Issue 1 ANNOUNCEMENT CANCER IMMUNOLOGY MINIATURES 1 An Exciting Collaboration between 26 T Cells Expressing Chimeric Antigen AACR and CRI Receptors Can Cause Anaphylaxis in Humans EDITORIAL Marcela V. Maus, Andrew R. Haas, Gregory L. Beatty, Steven M. Albelda, Bruce L. Levine, Xiaojun Liu, Yangbing Zhao, 2 A Message from the Founding Michael Kalos, and Carl H. June Editor-in-Chief Glenn Dranoff Synopsis: Chimeric antigen receptor–expressing T cells (CAR-T cells) represent a promising, novel MASTERS OF IMMUNOLOGY form of adoptive immunotherapy to overcome tolerance to cancer. Using an intermittent dosing 5 Logic of the Immune System schedule of autologous CAR-T cells electroporated Hidde L. Ploegh with mRNA encoding a hybrid single chain molecule comprising the extracellular domain of CANCER IMMUNOLOGY AT THE murine monoclonal antibody against human mesothelin and the human transmembrane and CROSSROADS: FUNCTIONAL GENOMICS cytoplasmic T-cell signaling domains, Maus and colleagues characterized and reported a serious 11 Getting Personal with Neoantigen- adverse event that occurred in one of four patients Based Therapeutic Cancer Vaccines receiving repeated modified T-cell infusions, with Nir Hacohen, Edward F. Fritsch, Todd A. Carter, proposed modifications to address and minimize Eric S. Lander, and Catherine J. Wu future adverse occurrence. MEETING REPORT RESEARCH ARTICLES 16 Tumor Immunology: Multidisciplinary 32 Anti-CTLA-4 Antibodies of IgG2a Science Driving Basic and Clinical Isotype Enhance Antitumor Activity Advances through Reduction of Intratumoral Bridget P. Keenan, Elizabeth M. Jaffee, and Regulatory T Cells Todd D.
    [Show full text]
  • Amino Acids Ross W
    Recognition of Class II MHC Peptide Ligands That Contain β-Amino Acids Ross W. Cheloha, Andrew W. Woodham, Djenet Bousbaine, Tong Wang, Shi Liu, John Sidney, Alessandro Sette, Samuel This information is current as H. Gellman and Hidde L. Ploegh of October 2, 2021. J Immunol published online 7 August 2019 http://www.jimmunol.org/content/early/2019/08/06/jimmun ol.1900536 Downloaded from Supplementary http://www.jimmunol.org/content/suppl/2019/08/06/jimmunol.190053 Material 6.DCSupplemental http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average by guest on October 2, 2021 Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2019 by The American Association of Immunologists, Inc. All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. Published August 7, 2019, doi:10.4049/jimmunol.1900536 The Journal of Immunology Recognition of Class II MHC Peptide Ligands That Contain b-Amino Acids Ross W. Cheloha,* Andrew W. Woodham,* Djenet Bousbaine,*,† Tong Wang,‡ Shi Liu,‡ John Sidney,x Alessandro Sette,x,{ Samuel H.
    [Show full text]
  • Qa-2–Dependent Selection of CD8 T Cell Receptor Cells in Murine
    Brief Definitive Report Qa-2–dependent Selection of CD8␣ր␣ T Cell Receptor ␣ր␤ϩ Cells in Murine Intestinal Intraepithelial Lymphocytes By Gobardhan Das,* Dina S. Gould,‡ Mathew M. Augustine,* Gladis Fragoso,¶ Edda Scitto,¶ Iwona Stroynowski,ʈ Luc Van Kaer,§ Danny J. Schust,‡ Hidde Ploegh,‡ and Charles A. Janeway, Jr.* From the *Section of Immunobiology, Yale University School of Medicine, and the Howard Hughes Medical Institute, New Haven, Connecticut 06520-8011; the ‡Department of Pathology, Harvard Medical School, Boston, Massachusetts 02115; the §Department of Microbiology and Immunology and The Howard Hughes Medical Institute, Vanderbilt University School of Medicine, Nashville, Tennessee 37232; the ʈCenter for Immunology and the Department of Microbiology and the Department of Internal Medicine, Southwestern Medical Center, Dallas, Texas 75235-9093; and the ¶Department of Immunology, Instituto de Investigaciones Biomedicas, Universidad Nacional Avtonoma de Mexico, Mexico D.F. 04510 Abstract Murine intestinal intraepithelial lymphocytes (iIELs) are made up of a heterogeneous mix of T cells with unique phenotypes. Whereas CD8ϩ T cells in peripheral lymphoid organs use CD8␣ր␤ and are selected on MHC class Ia molecules, a majority of iIELs use CD8␣ր␣. Here, we report that the presence of CD8␣ր␣ TCR-␣ր␤ cells in iIELs is independent of classical MHC class I molecules Kb and Db, as illustrated by their presence in Kb/Db double-knockout mice and in mice lacking a nonclassical MHC class I molecule, CD1d. Most strikingly, their in mice lacking transporter associated with antigen processing %70ف presence is decreased by (TAP). The TAP-dependent nonclassical MHC class I molecule Qa-2 is strongly implicated in the presence of these cells, as inferred from the low numbers of CD8␣ր␣ TCR-␣ր␤ T cells in mice deficient in Qa-2 genes.
    [Show full text]
  • BCRP Brochure 2021 Class
    Boston Combined Residency Program This brochure describes the residency program as we assume it will -19 exist will in be JulyThe 2021, Pediatric by which time Residency authorities Training Program predict a vaccine to COVID of available. If thatBoston is not the Children’s case and the Hospital pandemic is still active, the program Harvard Medical School will be very similar but many of the and educational conferences and other group activities Bostonwill be virtual Medical instead Center Boston University School of Medicine of in-person, as they are today. August 2020 edi,on CLASS OF 2021.. BOSTON COMBINED RESIDENCY PROGRAM Boston Medical Center Boston Children’s Hospital CONTENTS History…………........................... 3 Rotation # descriptions.................. 47# Global health fellowships............ 84# BCRP…........................................ 3# Night call................................... 53# Global health grants………….… 84 # Boston Children’s Hospital........... 3# Longitudinal ambulatory.............. 54# Diversity and Inclusion................. 84# Boston Medical Center................. 8# Electives………………………….. 55# Salaries and benefits.................... 87# People……................................... 11 Individualized curriculum............ 56# Child care................................... 88# Program director biosketches...... 11# Academic development block.. 56# O$ce of fellowship training....... 88# Residency program leadership..... 12# Education.................................... 57# Cost of living..............................
    [Show full text]
  • Dendritic Cells Compartments in + Tubulation of Class II Requirements
    Requirements for T Cell-Polarized Tubulation of Class II + Compartments in Dendritic Cells This information is current as Nicolas Bertho, Jan Cerny, You-Me Kim, Edda Fiebiger, of October 1, 2021. Hidde Ploegh and Marianne Boes J Immunol 2003; 171:5689-5696; ; doi: 10.4049/jimmunol.171.11.5689 http://www.jimmunol.org/content/171/11/5689 Downloaded from References This article cites 39 articles, 9 of which you can access for free at: http://www.jimmunol.org/content/171/11/5689.full#ref-list-1 http://www.jimmunol.org/ Why The JI? Submit online. • Rapid Reviews! 30 days* from submission to initial decision • No Triage! Every submission reviewed by practicing scientists • Fast Publication! 4 weeks from acceptance to publication *average by guest on October 1, 2021 Subscription Information about subscribing to The Journal of Immunology is online at: http://jimmunol.org/subscription Permissions Submit copyright permission requests at: http://www.aai.org/About/Publications/JI/copyright.html Email Alerts Receive free email-alerts when new articles cite this article. Sign up at: http://jimmunol.org/alerts The Journal of Immunology is published twice each month by The American Association of Immunologists, Inc., 1451 Rockville Pike, Suite 650, Rockville, MD 20852 Copyright © 2003 by The American Association of Immunologists All rights reserved. Print ISSN: 0022-1767 Online ISSN: 1550-6606. The Journal of Immunology Requirements for T Cell-Polarized Tubulation of Class II؉ Compartments in Dendritic Cells1 Nicolas Bertho,* Jan Cerny,†‡§ You-Me Kim,* Edda Fiebiger,* Hidde Ploegh,2* and Marianne Boes* Activation of naive CD4 T cells by dendritic cells requires the sequential interaction of many TCR molecules with peptide-class II complexes of the appropriate specificity.
    [Show full text]
  • A Herpesvirus Ubiquitin-Specific Protease Is Critical for Efficient T Cell Lymphoma Formation
    A herpesvirus ubiquitin-specific protease is critical for efficient T cell lymphoma formation Keith Jarosinski*, Lisa Kattenhorn†, Benedikt Kaufer*, Hidde Ploegh†‡, and Nikolaus Osterrieder*‡ *Department of Microbiology and Immunology, Cornell University, Ithaca, NY 14853; and †Whitehead Institute for Biomedical Research, Massachusetts Institute of Technology, 9 Cambridge Center, Cambridge, MA 02142 Edited by Elliott D. Kieff, Harvard Medical School, Boston, MA, and approved October 29, 2007 (received for review July 5, 2007) The herpesvirus ubiquitin-specific protease (USP) family, whose symptoms and their clockwork timing, as well as the availability founding member was discovered as a protease domain embedded of the natural host, make MDV an ideal model to assess the in the large tegument protein of herpes simplex virus 1 (HSV-1), is importance of the viral USP for alphaherpesvirus pathogenesis conserved across all members of the Herpesviridae. Whether this in vivo. The course of infection includes lytic replication, then conservation is indicative of an essential function of the enzyme in latency, followed by reactivation and the formation of massive T vivo has not yet been established. As reported here, USP activity is cell lymphomas in multiple organs (18–20). We undertook the conserved in Marek’s disease virus (MDV), a tumorigenic alpha- generation of an MDV mutant that lacks the USP activity to herpesvirus. A single amino acid substitution that abolishes the address its role in pathogenicity. USP activity of the MDV large tegument protein diminishes MDV replication in vivo, and severely limits the oncogenic potential of Results the virus. Expression of the USP transcripts in MDV-transformed Characterization of the MDVUSP Active Site Cysteine.
    [Show full text]
  • Supporting Information
    Supporting Information Sanderson et al. 10.1073/pnas.1614472114 SI Materials and Methods verified expression at the establishment of the cell line and Mice and Primary Immune Cells. A full list of primary immune cells subsequently periodically checked the expression of the antigen − − used in the study is presented in Table S2. C57BL/6 and Rag / by immunofluorescence flow cytometry (see Table S1). Adherent mice were bred in the University of Basel Mouse Core Facility. cells were washed in PBS, trypsinized, resuspended in complete − − Tg(TcraTcrb)425Cbn (OT-II) mice on a Rag2 / background medium, washed in labeling buffer (PBS containing 2% FCS by were a kind gift from Ed Palmer, University of Basel. FluBI mice volume and 1 g/l of sodium azide), incubated for 30 min on ice in (23) were bred from founder members provided by Stephanie primary antibody, washed again, incubated with fluorescent Dougan and Hidde Ploegh, Whitehead Institute, Cambridge, secondary antibodies, washed, and measured on a BD LSR MA. IgHMOG (ref. 12; also known as Th B cells) and 2D2 mice Fortessa flow cytometer (BD Biosciences). Cells known to be (13) were bred from founder members provided by Guru positive and negative for the target antigen were labeled and Krishnamoorthy and Hartmut Wekerle, Max-Planck-Institut für measured in parallel each time. A typical result from such an Neurobiologie, Martinsried, Germany. Primary immune cells experiment is shown in Fig. 4E. When flow cytometry results are were obtained from spleens by mechanical disruption followed shown as contour plots, the contour lines are spaced at 10%. by brief settlement under gravity to remove tissue fragments.
    [Show full text]
  • Improved Antitumor Efficacy of Chimeric Antigen Receptor T Cells That Secrete Single-Domain Antibody Fragments
    Published OnlineFirst February 4, 2020; DOI: 10.1158/2326-6066.CIR-19-0734 CANCER IMMUNOLOGY RESEARCH | RESEARCH ARTICLE Improved Antitumor Efficacy of Chimeric Antigen Receptor T Cells that Secrete Single-Domain Antibody Fragments Yushu Joy Xie1,2, Michael Dougan3, Jessica R. Ingram4,†, Novalia Pishesha1,2, Tao Fang1, Noor Momin2,5, and Hidde L. Ploegh1 ABSTRACT ◥ Chimeric antigen receptor (CAR) T-cell therapy is effective in the that can endow CAR T cells with desirable properties. The secretion treatment of cancers of hematopoietic origin. In the immunosup- of an anti-CD47 VHH by CAR T cells improves engagement of the pressive solid tumor environment, CAR T cells encounter obstacles innate immune system, enables epitope spreading, and can enhance that compromise their efficacy. We developed a strategy to address the antitumor response. CAR T cells that secrete anti–PD-L1 or these barriers by having CAR T cells secrete single-domain antibody anti–CTLA-4 nanobodies show improved persistence and demon- fragments [variable heavy domain of heavy chain antibodies (VHH) strate the versatility of this approach. Furthermore, local delivery of or nanobodies] that can modify the intratumoral immune landscape secreted anti-CD47 VHH-Fc fusions by CAR T cells at the tumor and thus support CAR T-cell function in immunocompetent ani- site limits their systemic toxicity. CAR T cells can be further mals. VHHs are small in size and able to avoid domain swapping engineered to simultaneously secrete multiple modalities, allowing when multiple nanobodies are expressed simultaneously—features for even greater tailoring of the antitumor immune response. Introduction cytokines such as IL12, IL15, and IL18 can modify the tumor micro- environment, make it more hospitable for T-cell activity, and thus Chimeric antigen receptor (CAR) T-cell therapy relies on T cells promote an antitumor response (9–11).
    [Show full text]
  • Engineered Erythrocytes Covalently Linked to Antigenic Peptides Can Protect Against Autoimmune Disease,” by Novalia Pishesha, Angelina M
    Correction IMMUNOLOGY AND INFLAMMATION Correction for “Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease,” by Novalia Pishesha, Angelina M. Bilate, Marsha C. Wibowo, Nai-Jia Huang, Zeyang Li, Rhogerry Dhesycka, Djenet Bousbaine, Hojun Li, Heide C. Patterson, Stephanie K. Dougan, Takeshi Maruyama, Harvey F. Lodish, and Hidde L. Ploegh, which appeared in issue 12, March 21, 2017, of Proc Natl Acad Sci USA (114: 3157–3162; first published March 7, 2017; 10.1073/pnas.1701746114). The authors note that the author name Rhogerry Dhesycka should instead appear as Rhogerry Deshycka. The corrected au- thor line appears below. The online version has been corrected. Novalia Pishesha, Angelina M. Bilate, Marsha C. Wibowo, Nai-Jia Huang, Zeyang Li, Rhogerry Deshycka, Djenet Bousbaine, Hojun Li, Heide C. Patterson, Stephanie K. Dougan, Takeshi Maruyama, Harvey F. Lodish, and Hidde L. Ploegh www.pnas.org/cgi/doi/10.1073/pnas.1705149114 CORRECTION www.pnas.org PNAS | April 25, 2017 | vol. 114 | no. 17 | E3583 Downloaded by guest on September 28, 2021 Engineered erythrocytes covalently linked to antigenic peptides can protect against autoimmune disease Novalia Pisheshaa,b,c, Angelina M. Bilatea, Marsha C. Wibowoa,d, Nai-Jia Huanga, Zeyang Lia,c, Rhogerry Dhesyckaa,b, Djenet Bousbainea,c,d, Hojun Lia, Heide C. Pattersona, Stephanie K. Dougana, Takeshi Maruyamaa, Harvey F. Lodisha,b,d,1, and Hidde L. Ploegha,c,d,1,2 aWhitehead Institute for Biomedical Research, Cambridge, MA 02142; bDepartment of Biological Engineering, Massachusetts Institute of Technology, Cambridge, MA 02142; cProgram in Cellular and Molecular Medicine, Boston Children’s Hospital, Boston, MA 02115; and dDepartment of Biology, Massachusetts Institute of Technology, Cambridge, MA 02142 Contributed by Hidde L.
    [Show full text]