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Microbial Enzymes and Their Applications in Industries and Medicine
BioMed Research International Microbial Enzymes and Their Applications in Industries and Medicine Guest Editors: Periasamy Anbu, Subash C. B. Gopinath, Arzu Coleri Cihan, and Bidur Prasad Chaulagain Microbial Enzymes and Their Applications in Industries and Medicine BioMed Research International Microbial Enzymes and Their Applications in Industries and Medicine Guest Editors: Periasamy Anbu, Subash C. B. Gopinath, Arzu Coleri Cihan, and Bidur Prasad Chaulagain Copyright © 2013 Hindawi Publishing Corporation. All rights reserved. This is a special issue published in “BioMed Research International.” All articles are open access articles distributed under the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited. Contents Microbial Enzymes and Their Applications in Industries and Medicine,PeriasamyAnbu, Subash C. B. Gopinath, Arzu Coleri Cihan, and Bidur Prasad Chaulagain Volume 2013, Article ID 204014, 2 pages Effect of C/N Ratio and Media Optimization through Response Surface Methodology on Simultaneous Productions of Intra- and Extracellular Inulinase and Invertase from Aspergillus niger ATCC 20611, Mojdeh Dinarvand, Malahat Rezaee, Malihe Masomian, Seyed Davoud Jazayeri, Mohsen Zareian, Sahar Abbasi, and Arbakariya B. Ariff Volume 2013, Article ID 508968, 13 pages A Broader View: Microbial Enzymes and Their Relevance in Industries, Medicine, and Beyond, Neelam Gurung, Sumanta Ray, Sutapa Bose, and Vivek Rai Volume 2013, Article -
Amyloid-Degrading Ability of Nattokinase from Bacillus Subtilis Natto
J. Agric. Food Chem. 2009, 57, 503–508 503 Amyloid-Degrading Ability of Nattokinase from Bacillus subtilis Natto †,‡ § § † RUEI-LIN HSU, KUNG-TA LEE, JUNG-HAO WANG, LILY Y.-L. LEE, AND ,†,‡ RITA P.-Y. CHEN* Institute of Biological Chemistry, Academia Sinica, Taipei 115, Taiwan, R. O. C., Institute of Biochemical Sciences, National Taiwan University, Taipei 106, Taiwan, R. O. C., and Department of Biochemical Science and Technology, National Taiwan University, Taipei 106, Taiwan, R. O. C. More than 20 unrelated proteins can form amyloid fibrils in vivo which are related to various diseases, such as Alzheimer’s disease, prion disease, and systematic amyloidosis. Amyloid fibrils are an ordered protein aggregate with a lamellar cross- structure. Enhancing amyloid clearance is one of the targets of the therapy of these amyloid-related diseases. Although there is debate on whether the toxicity is due to amyloids or their precursors, research on the degradation of amyloids may help prevent or alleviate these diseases. In this study, we explored the amyloid-degrading ability of nattokinase, a fibrinolytic subtilisin-like serine protease, and determined the optimal conditions for amyloid hydrolysis. This ability is shared by proteinase K and subtilisin Carlsberg, but not by trypsin or plasmin. KEYWORDS: Nattokinase; amyloid; natto; subtilisin NAT; amyloid degradation; fibril; amyloidosis INTRODUCTION of cardiovascular disease. Dietary supplementation with natto Natto, a fermented food made from boiled soybeans, has suppresses the intimal thickening of arteries and leads to the been eaten for more than 1000 years in Asia. The fermenta- lysis of mural thrombi seen after endothelial injury (12). tion microbe isolated from natto is the Gram-positive Other clinically thrombolytic agents, such as urokinase and endospore-forming bacterium Bacillus subtilis natto (formerly streptokinase, are costly and unstable in the intestinal tract designated Bacillus natto)(1). -
Identification and Phylogenetic Analysis of Keratinase Producing Bacteria SNP1 from Poultry Field
International Journal of Biotechnology and Biochemistry ISSN 0973-2691 Volume 15, Number 1 (2019) pp. 39-51 © Research India Publications http://www.ripublication.com Identification and Phylogenetic Analysis of Keratinase Producing Bacteria SNP1 from Poultry Field Divya Balakrishnan and Nithadas Sathyadas Padmanabhan Department of Biotechnology, Sree Narayana College, Kollam-691001, Kerala, India. (*Corresponding author) Abstract The study was conduct to select the best promising keratinolytic bacterial strain. A keratinase positive bacterial strain was isolated from the soil samples of poultry field, Attingal, Thiruvananthapuram. Each sample was plated on skim milk agar and feather meal agar plates containing 5 g feather. The well grown isolates which produced the largest clear zone on skimmed milk plate were selected for keratinase assays. Out of 26 bacterial isolates, 7 isolates were selected. Among the selected strain, the best keratinase producing bacterium SNP1was selected for further analysis. The SNP1 potential strain was later confirmed as Bacillus haynessi based on molecular and phylogenetic analysis. The medium components and culture conditions were optimized to enhance keratinase production through optimization. Keratin and feather powder (10g/l) were identified as good substrates for the highest keratinase production. The strain SNP1 resulted maximum enzyme production at 96h of incubation at 37°C and pH 8 under 120 rpm. Therefore, Bacillus hayneisi might be used for large scale production of keratinase for industrial purposes. Keywords: Keratinase, Bacillus sp., Optimization, Enzyme activity, 16SrRNA Keratin is a hard-degrading fibrous and recalcitrant structural protein, which forms the third most abundant polymer in nature after cellulose and chitin (Lene et al ., 2016). In the native state, the feather keratin resists the degradation by proteolytic enzymes such as trypsin and pepsin due to tightpeptide-chains held together by disulfide bridges by means of cysteine residues (Weeranut, 2017; Korniłłowicz-Kowalska and Bohacz, 2011). -
Pupils Our Opinions Parents Teachers
Activity: Pair game Level: 5th and 6th Activity: Double, triple … Level: 5th and 6th Material: OUR OPINIONS Material: Spanish deck and Maths notebook. Spanish deck: cards from 1 to 4 and the fig- "We can not teach anything to anyone. We Number of players: ures. The numerical cards have their own Four, although it can be adapted to any number of can only help them discover for themselves " value; the jack multiplies by 2, the horse by 3 PUPILS If we play with the deck of cards, we play players. and the king by 4) Galileo Galilei Ideally, various groups are made throughout the with our friends, and my mom says it's better Number of players: 2 to 4 players class so that they can answer the questions. Game development: than playing with the Play Station, and we Game development: 1. The 28 cards are shuffled and distributed to I like playing with the 1. All cards are shuffled and placed randomly face the players (it is not essential that everyone are learning maths too (Rocío, 10 years) down on a table. has the same number) card deck because I like 2. The first player picks up two cards and places 2. The first player, with the deck in one hand, quessing numbers and them face up. If they are a pair * remove them face down, takes the first card and places it on working with them to It´s the best moment of the day when and try again; if they are not, place them upside top of the table. -
Tubular Heaters Heat up the Foodservice Industry
FOODSERVICE TUBULAR HEATERS Tubular Heaters Heat Up the Foodservice Industry When there’s a need for heat in your foodservice equipment Applications application—from fryers to griddles to ovens—turn to Watlow, the leader with more than 25 years experience providing • Griddles heating solutions to the foodservice equipment market. Using • Rotisserie ovens • Convection ovens Watlow’s WATROD round tubular and FIREBAR® flat tubular heating elements in the cooking process promotes faster • Combi ovens cooking, consistent food preparation, ease of operation and • Conveyor ovens low maintenance. • Fryers • Steamers These reliable, versatile heating elements can be configured • Smokers with a variety of wattage and voltage ratings, terminations, • Warewashers sheath materials and mounting options to satisfy the most • Warming cabinets demanding foodservice applications. • Toasters • Other clamp-on applications Advantages • Watlow’s heaters for the foodservice industry are constructed with epoxy or silicone seals to combat moisture contamination from environmental kitchen conditions. • Compacted MgO insulation transfers heat away from resistance wire to sheath material and media more efficiently resulting in faster heat up. • Over 36 standard and a virtually limitless array of custom bend formation options enable the heating element to be HAN-FTH-0807 designed around available space to maximize heating efficiency. To be automatically connected to the nearest North American Technical and Sales Office: 1-800-WATLOW2 • www.watlow.com • [email protected] -
Horseshoe Crab Limulus Polyphemus
Supplemental Volume: Species of Conservation Concern SC SWAP 2015 Atlantic Horseshoe Crab Limulus polyphemus Contributor (2005): Elizabeth Wenner (SCDNR) Reviewed and Edited (2013): Larry Delancey and Peter Kingsley-Smith [SCDNR] DESCRITPION Taxonomy and Basic Description Despite their name, horseshoe crabs are not true crabs. The Atlantic horseshoe crab, Limulus polyphemus, is the only member of the Arthropoda subclass Xiphosura found in the Atlantic. Unlike true crabs, which have 2 pairs of antennae, a pair of jaws and 5pairs of legs, horseshoe crabs lack antennae and jaws and have 7 pairs of legs, including a pair of chelicerae. Chelicerae are appendages similar to those used by spiders and scorpions for grasping and crushing. In addition, horseshoe crabs have book lungs, similar to spiders and different from crabs, which have gills. Thus, horseshoe crabs are more closely related to spiders and scorpions than they are to other crabs. Their carapace is divided into three sections: the anterior portion is the prosoma; the middle section is the opithosoma; and the “tail” is called the telson. Horseshoe crabs have two pairs of eyes located on the prosoma, one anterior set of simple eyes, and one set of lateral compound eyes similar to those of insects. In addition, they possess a series of photoreceptors on the opithosoma and telson (Shuster 1982). Horseshoe crabs are long-lived animals. After attaining sexual maturity at 9 to 12 years of age, they may live for another 10 years or more. Like other arthropods, horseshoe crabs must molt in order to grow. As the horseshoe crab ages, more and more time passes between molts, with 16 to 19 molts occurring before a crab becomes mature, stops growing, and switches energy expenditure to reproduction. -
Commercial Electric Water Heaters
Service Handbook COMMERCIAL ELECTRIC WATER HEATERS MODELS CSB52**I, CSB82**I, CSB120**I & CSB52**S, CSB82**S, CSB120**S INSTALLATION CONSIDERATIONS - PRE SERVICE CHECKS - WATER HEATER CONSTRUCTION - 500 Tennessee Waltz Parkway OPERATION & SERVICE - TROUBLESHOOTING Ashland City, TN 37015 SERVICING SHOULD ONLY BE PERFORMED BY A QUALIFIED SERVICE AGENT. PRINTED IN THE U.S.A 1008 315015-000 1 COMMERCIAL ELECTRIC WATER HEATER SERVICE MANUAL TABLE OF CONTENTS INTRODUCTION .......................................................................2 ELECTRONIC CONTROLS..................................................... 41 Qualifications ......................................................................2 CCB - Central Control Board............................................ 42 Service Warning .................................................................2 CCB Socket & Wiring Terminal Identification ............ 43 Tools Required....................................................................3 CCB Enable Disable Circuit(s) Test........................... 45 INSTALLATION CONSIDERATIONS ........................................4 Checking Power And Ground To The CCB ............... 46 Closed Water Systems .......................................................4 UIM - User Interface Module ............................................ 47 Thermal Expansion.............................................................4 ELECTRONIC CONTROL SYSTEM ....................................... 48 Electrical Requirements......................................................5 -
A Forgotten Confederate: John H. Ash's Story Rediscovered
Georgia Southern University Digital Commons@Georgia Southern Electronic Theses and Dissertations Graduate Studies, Jack N. Averitt College of Spring 2017 A Forgotten Confederate: John H. Ash's Story Rediscovered Heidi Moye Follow this and additional works at: https://digitalcommons.georgiasouthern.edu/etd Part of the Military History Commons, and the United States History Commons Recommended Citation Moye, Heidi, "A Forgotten Confederate: John H. Ash's Story Rediscovered" (2017). Electronic Theses and Dissertations. 1565. https://digitalcommons.georgiasouthern.edu/etd/1565 This thesis (open access) is brought to you for free and open access by the Graduate Studies, Jack N. Averitt College of at Digital Commons@Georgia Southern. It has been accepted for inclusion in Electronic Theses and Dissertations by an authorized administrator of Digital Commons@Georgia Southern. For more information, please contact [email protected]. A FORGOTTEN CONFEDERATE: JOHN H. ASH’S STORY REDISCOVERED by HEIDI MOYE (Under the Direction of Anastatia Sims) ABSTRACT A historical study of a southern family living in Savannah, GA from shortly before the election of 1860 through the Civil War years based on the journals of John Hergen Ash II (1843-1918). INDEX WORDS: John Hergen Ash, Savannah, GA, Antebellum South, Civil War, 5th Georgia Cavalry, Georgia Hussars, Estella Powers Ash, Laura Dasher Ash, Eutoil Tallulah Foy Ash A FORGOTTEN CONFEDERATE: JOHN H. ASH’S STORY REDISCOVERED by HEIDI MOYE B. A., Georgia Southern University, 2012 A Thesis Submitted to the Graduate Faculty of Georgia Southern University in Partial Fulfillment of the Requirements for the Degree MASTER OF ARTS STATESBORO, GEORGIA © 2017 HEIDI MOYE All Rights Reserved 1 A FORGOTTEN CONFEDERATE: JOHN H. -
The GNOME Census: Who Writes GNOME?
The GNOME Census: Who writes GNOME? Dave Neary & Vanessa David, Neary Consulting © Neary Consulting 2010: Some rights reserved Table of Contents Introduction.........................................................................................3 What is GNOME?.............................................................................3 Project governance...........................................................................3 Why survey GNOME?.......................................................................4 Scope and methodology...................................................................5 Tools and Observations on Data Quality..........................................7 Results and analysis...........................................................................10 GNOME Project size.......................................................................10 The Long Tail..................................................................................11 Effects of commercialisation..........................................................14 Who does the work?.......................................................................15 Who maintains GNOME?................................................................17 Conclusions........................................................................................22 References.........................................................................................24 Appendix 1: Modules included in survey...........................................25 2 Introduction What -
Contemporary Art Magazine Issue # Sixteen December | January Twothousandnine Spedizione in A.P
contemporary art magazine issue # sixteen december | january twothousandnine Spedizione in a.p. -70% _ DCB Milano NOVEMBER TO JANUARY, 2009 KAREN KILIMNIK NOVEMBER TO JANUARY, 2009 WadeGUYTON BLURRY CatherineSULLIVAN in collaboration with Sean Griffin, Dylan Skybrook and Kunle Afolayan Triangle of Need VibekeTANDBERG The hamburger turns in my stomach and I throw up on you. RENOIR Liquid hamburger. Then I hit you. After that we are both out of words. January - February 2009 DEBUSSY URS FISCHER GALERIE EVA PRESENHUBER WWW.PRESENHUBER.COM TEL: +41 (0) 43 444 70 50 / FAX: +41 (0) 43 444 70 60 LIMMATSTRASSE 270, P.O.BOX 1517, CH–8031 ZURICH GALLERY HOURS: TUE-FR 12-6, SA 11-5 DOUG AITKEN, EMMANUELLE ANTILLE, MONIKA BAER, MARTIN BOYCE, ANGELA BULLOCH, VALENTIN CARRON, VERNE DAWSON, TRISHA DONNELLY, MARIA EICHHORN, URS FISCHER, PETER FISCHLI/DAVID WEISS, SYLVIE FLEURY, LIAM GILLICK, DOUGLAS GORDON, MARK HANDFORTH, CANDIDA HÖFER, KAREN KILIMNIK, ANDREW LORD, HUGO MARKL, RICHARD PRINCE, GERWALD ROCKENSCHAUB, TIM ROLLINS AND K.O.S., UGO RONDINONE, DIETER ROTH, EVA ROTHSCHILD, JEAN-FRÉDÉRIC SCHNYDER, STEVEN SHEARER, JOSH SMITH, BEAT STREULI, FRANZ WEST, SUE WILLIAMS DOUBLESTANDARDS.NET 1012_MOUSSE_AD_Dec2008.indd 1 28.11.2008 16:55:12 Uhr Galleria Emi Fontana MICHAEL SMITH Viale Bligny 42 20136 Milano Opening 17 January 2009 T. +39 0258322237 18 January - 28 February F. +39 0258306855 [email protected] www.galleriaemifontana.com Photo General Idea, 1981 David Lamelas, The Violent Tapes of 1975, 1975 - courtesy: Galerie Kienzie & GmpH, Berlin L’allarme è generale. Iper e sovraproduzione, scialo e vacche grasse si sono tra- sformati di colpo in inflazione, deflazione e stagflazione. -
Release 2021-03
Metrics Release 2021-03 https://chaoss.community/metrics MIT License Copyright © 2021 CHAOSS a Linux Foundation® Project CHAOSS Contributors include: Aastha Bist, Abhinav Bajpai, Ahmed Zerouali, Akshara P, Akshita Gupta, Amanda Brindle, Anita Ihuman, Alberto Martín, Alberto Pérez García-Plaza, Alexander Serebrenik, Alexandre Courouble, Alolita Sharma, Alvaro del Castillo, Ahmed Zerouali, Amanda Casari, Amy Marrich, Ana Jimenez Santamaria, Andre Klapper, Andrea Gallo, Andy Grunwald, Andy Leak, Aniruddha Karajgi, Anita Sarma, Ankit Lohani, Ankur Sonawane, Anna Buhman, Armstrong Foundjem, Atharva Sharma, Ben Lloyd Pearson, Benjamin Copeland, Beth Hancock, Bingwen Ma, Boris Baldassari, Bram Adams, Brian Proffitt, Camilo Velazquez Rodriguez, Carol Chen, Carter Landis, Chris Clark, Christian Cmehil- Warn, Damien Legay, Dani Gellis, Daniel German, Daniel Izquierdo Cortazar, David A. Wheeler, David Moreno, David Pose, Dawn Foster, Derek Howard, Don Marti, Drashti, Duane O’Brien, Dylan Marcy, Eleni Constantinou, Elizabeth Barron, Emily Brown, Emma Irwin, Eriol Fox, Fil Maj, Gabe Heim, Georg J.P. Link, Gil Yehuda, Harish Pillay, Harshal Mittal, Henri Yandell, Henrik Mitsch, Igor Steinmacher, Ildiko Vancsa, Jacob Green, Jaice Singer Du Mars, Jaskirat Singh, Jason Clark, Javier Luis Cánovas Izquierdo, Jeff McAffer, Jeremiah Foster, Jessica Wilkerson, Jesus M. Gonzalez- Barahona, Jilayne Lovejoy, Jocelyn Matthews, Johan Linåker, John Mertic, Jon Lawrence, Jonathan Lipps, Jono Bacon, Jordi Cabot, Jose Manrique Lopez de la Fuente, Joshua Hickman, Joshua -
PDF (611K, 38 Pages)
Getting Unstuck A Sampler of Advice for Open Source Projects by Sumana Harihareswara © 2020 Sumana Harihareswara under the Creative Commons Attribution-ShareAlike 4.0 license (CC BY-SA) Please feel free to share this book, translate it, and reuse it per the license.1 Sumana Harihareswara Changeset Consulting LLC P.O. Box 721160 Jackson Heights, NY 11372 https://changeset.nyc/ +1 (929) 255-4578 Written in emacs and in New York City, 2020. Cover design and layout by Julia Rios Cover photograph by Susanne Stöckli For Leonard, my foundation. And for Aaron Swartz, our lighthouse. Table of Contents • Introduction • Conducting a SWOT analysis • How to start thinking about budgets and money • Teaching and including unskilled volunteers • An outline of the full book • Acknowledgments • About the author • Feedback welcome Introduction Getting Open Source Projects Unstuck (or, in other words: maintaining legacy open source projects. Below is the introduction for the full, forthcoming book.) Who this book is for and what you should get out of it You are about to get an open source project unstuck. Maybe a bunch of work is piling up in the repository and users are getting worried, waiting for a release. Maybe developers have gotten bogged down, trying to finish a big rewrite while maintaining the stable release. Maybe the project's suffering for lack of infrastructure — testing, money, an institutional home. You noticed the problem. So that means it's up to you to fix it. Or you're getting paid to fix it, even though you didn't start this thing. A while ago I blurted out the phrase "dammit-driven leadership." Because sometimes you look around, and you realize something needs doing, and you're the only one who really gets why, so you say, “Dammit, okay, I'll do it, then.” After reading this book, you should be prepared to: 1.