LPAR4 Antibody

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Product Datasheet LPAR4 Antibody Catalog No: #31253 Package Size: #31253-1 50ul #31253-2 100ul Orders: [email protected] Support: [email protected] Description Product Name LPAR4 Antibody Host Species Rabbit Clonality Polyclonal Applications ELISA WB IHC Species Reactivity Hu Ms Rt Specificity The antibody detects endogenous level of total LPAR4 protein. Immunogen Type Peptide Immunogen Description Synthetic peptide corresponding to a region derived from 358-370 amino acids of Human lysophosphatidic acid receptor 4 Target Name LPAR4 Other Names lysophosphatidic acid receptor 4, LPA4, P2Y9, GPR23, P2RY9, P2Y5-LIKE Accession No. Swiss-Prot:P25116Gene ID:2149 Formulation Rabbit IgG in pH7.4 PBS, 0.05% NaN3, 40% Glycerol. Storage Store at -20°C/1 year Application Details Predicted MW: 42kd ELISA: 1:1000-1:5000 Western blotting: 1:500-1:2000 Immunohistochemistry: 1:25-1:100 Images Gel: 10%SDS-PAGE Lane1: Human leiomyosarcoma tissue lysate Lane2: Human liver cancer tissue lysate Lysates: 40 ug per lane Primary antibody: 1/450 dilution Secondary antibody: Goat anti Rabbit IgG - H&L (HRP) at 1/10000 dilution Exposure time: 30 seconds Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 1 The image on the left is immunohistochemistry of paraffin-embedded Human esophagus cancer tissue using 31253 (LPAR4 Antibody) at dilution 1/30, on the right is treated with the synthetic peptide. Background This gene encodes a member of the lysophosphatidic acid receptor family. It may also be related to the P2Y receptors, a family of receptors that bind purine and pyrimidine nucleotides and are coupled to G proteins. The encoded protein may play a role in monocytic differentiation. Note: This product is for in vitro research use only and is not intended for use in humans or animals. Address: 8400 Baltimore Ave., Suite 302, College Park, MD 20740, USA http://www.sabbiotech.com 2.

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Lysophosphatidic Acid and Its Receptors: Pharmacology and Therapeutic Potential in Atherosclerosis and Vascular Disease
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Supplementary Information Supplementary Table 1. List of polypeptide cell surface receptor and their cognate ligand genes. Supplementary Table 2. List of coding SNPs with high FST ( > 0.5) in human GPCR and their cognate ligand genes. Supplementary Table 3. List of coding SNPs with high FST ( > 0.5) in human nonGPCR receptor and ligand genes. Supplementary Table 4. List of genotyped SNPs from 44246961 to 44542055 on chromosome 17. The HapMap II dataset was analyzed using HaploView. The 101 SNPs included in LD plots of Supplementary Fig. 3 (A-C) are highlighted by a grey background. The 37 SNPs used in the haplotype analysis of Fig. 2C are indicated by red letters. SNPs that are linked with rs2291725 are indicated by bold red letters. Supplementary Table 5. Allele frequency of rs2291725 in the HGDP-CEPH populations. Frequencies of GIP103T and GIP103C alleles in each of the 52 populations from the seven geographical regions and the number of chromosomes analyzed for each population. 1 Supplementary Table 6. EC50 values for GIP receptor activation at four different time points after incubation with pooled human serum or pooled complement-preserved human serum (N=4). Supplementary Table 7. EC50 values for GIP receptor activation at three different time points after incubation with a recombinant DPP IV enzyme (N=4). Supplementary Fig. 1. Cumulative distribution function (CDF) plots for the FST of coding SNPs in human GPCRs and their cognate ligand genes (blue) and all other human genes (magenta). The FST was computed between three HapMap II populations (CEU, YRI, and ASN), and coding SNPs have been split into synonymous and nonsynonymous groups.
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