PN 12090
Beginner’s Guide to Vevo® 2100
December 2008
Beginner’s Guide to the Vevo® 2100 Rev. 1.4
This Beginner’s Guide to Vevo 2100 provides a quick overview of the Vevo 2100 imaging modes and provides key tips to allow users to start a study, acquire images, make measurements and export data. A more detailed step-by-step description of the system is available in the Vevo 2100 Operator Manual.
Connect the transducer Attach the transducer that will be used during the imaging session to the connector on the front panel of the Vevo 2100.
Start the Vevo 2100 1. Turn on the Vevo system using the breaker switch at the back of the system and then the computer by clicking the standby switch on the left side of the system.
2. Wait for the Windows Vista operating system to start-up. The system is configured by VisualSonics® to automatically log in. The Vevo 2100 software should automatically launch.
Start the Vevo 1. When a scanhead is connected the transducer initialization window appears. Software 2. Select the appropriate application and click Initialize. Initialization sets up the appropriate preset parameters for the selected application and specific transducer.
Fig.1 – Initialization Dialog
2. Once the transducer is initialized, the application opens in the Study Browser. The Study Browser window is used for sorting acquired data, for review and data export.
Beginner’s Guide to the Vevo® 2100 Rev. 1.4
Click the New button in the Study Browser to open the “New Study” window. In this window the user can start a brand new study or a new series; a series is a new session that could be part of an old study.
Fig.2 – New Study dialog
3. Select one of the options. The application is going to open the Study Info window where the user needs to enter required information in order to start the study. The fields marked with yellow have to be filled in before the user can start imaging.
Fig.3 – Study/Series Information
4. Once all the required information is entered press OK and the software automatically begins imaging in B-Mode. There are various imaging modes available, accessible by the mode buttons located on the keyboard
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 3 of 12 B-Mode B-Mode imaging is used to visualize anatomical structures in a 2D greyscale image.
1. Press B-Mode to start acquistion. The acquisition parameters panel is activated by default. Users can change the parameters as needed and can save a custom set by pressing Save Presets.
2. Press
4. To store a cineloop press
5. To store a single frame from a live acquisition or a frozen acquisition press .
6. Users can simultaneously label and save a cine loop: by pressing Image Label, enter the Image name, and then press enter.
Fig.4 – B-Mode acquisition window – left kidney in transverse view
B-Mode is available on all Vevo 2100 systems, the following imaging modes are available as options to the Vevo 2100. You will only be able to use the options that have been purchased with your system.
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 4 of 12 M-Mode M-Mode imaging is used for visualizing and quantifying tissue movement over time, for example the motion of the myocardium over time.
1. In B-Mode acquisition, optimise the image for the area to be imaged in M-Mode.
2 Press the M-Mode button on the keyboard to display the M- Mode overlay. Adjust the position by moving the trackball, and size by clicking the SV Gate toggle up or down. Press the M- Mode button again or click the right mouse button. 3 Start and stop data acquisition using
Fig.5 – M-Mode acquisition window – Left Ventricle short axis view
Fig. 5 – M-Mode Acquisition Window – Abdominal Aorta
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 5 of 12 Pulsed Wave (PW) PW Doppler imaging is used for visualizing and quantifying Doppler Mode blood flow through vessels and values in the heart.
1. In B-Mode acquisition, locate the region of interest for PW Doppler acquisition. Ensure that the angle between the direction of blood flow and the transmitted ultrasound beam is 60o or less.
2. Press the PW button on the keyboard. 3. Position and size the sample volume at the desired location for PW Doppler signal acquisition; use the trackball to change the sample volume position and the SV Gate toggle to change the size of the sample volume.
4. Adjust the PW Doppler angle using the Doppler Angle knob the yellow dotted line should be placed in line with the blood flow. If steering is needed to reduce the angle between blood flow and ultrasound beam use the Beam Angle toggle to steer the angle either to the right or left.
5. Press the right “mouse” button or the PW button again to switch to the PW Doppler acquisition window.
6. Start and stop data acquisition using
7. Use the Cine Loop Review knob to scroll through the acquired data.
8. To store a cineloop press
9. To store a still image press .
Fig.6 – PW Doppler acquisition window – Mitral Valve apical four chamber view
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 6 of 12 3D-Mode 3D-Mode imaging is used to visualize an anatomical structure in three dimensions. Multiple 2D slices are acquired and compiled into a 3D volume. 3D-Mode imaging can be used for B-Mode Power Doppler Mode, and Contrast Mode Data.
1. Connect the 3D motor stage to the Vevo Rail System, attach the motor to the ball joint, where the scanhead clamp can also be attached.
2. Connect the transducer clamp to the 3D motor. 3. Mount the transducer in the clamp; the transducer imaging plane must be perpendicular to the axis along which the motor will move.
4. Press the <3D> button on the keyboard. The 3D motor initialization dialog will come up. Click okay and the motor will initialize. Ensure that the probe is not touching an animal and that nothing will abstruct it’s movement.
5. Optimize the image for acquisition, for example in B-Mode and determine the range the motor must move to cover the desired volume using the micromanipulators on the stage for the animal handling platform.
6. When the acquisition ends the application opens the 3D analysis window. If using Contrast Mode, the image is loaded into a 2D window frame which can then be loaded into 3D once all post processing steps are complete; this is done by clicking Load in 3D.
7. To store the data press <3D Store> or Auto-Save on Image Label.
Fig.7 – 3D Mode analysis window – Left Ventricle using ECG and Respiration gating
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 7 of 12 Power Doppler Mode Power Doppler Mode imaging is used to visualize blood flow; generally Power Doppler is used to visualize flow in structures such as tumors or abdominal organs.
1. In B-Mode imaging optimize the image for the target of interest to be imaged in Power Doppler.
2. Press the Power button on the keyboard to start acquisition 3. Position and size the Power Box at the desired location for Power Doppler signal acquisition. The position (solid box) of the Power Box can be adjusted by moving the Trackball around the image, the size (dotted box) can also be adjusted by using the trackball; to toggle between the two boxes use the right “mouse” button.
4. Start and stop acquisition using
6. To store a still image press . Note: Power Doppler Mode is also supported in 3D-Mode. To acquire Power-3D-Mode data, from Power Doppler Mode click 3D and adjust the 3D acqusition parameters. At the end of the acquisition the application switches to Power 3D analysis window.
Fig.8 – Power Doppler acquisition window – Subcutaneous Tumor on hind limb
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 8 of 12 Color Doppler Mode Color Dopper imaging is used to visualize blood flow and give additional information about the direction of flow. Color Doppler is used on a wide range of targets such as vessels, the heart, and even tumors and abdominal organs
1. In B-Mode imaging optimize the image for the target of interest to be imaged in Color Doppler.
2. Press the Color button on the keyboard to start acquisition. 3. Position and size the Color Box at the desired location for Color Doppler signal acquisition. The position (solid box) of the Color Box can be adjusted by moving the Trackball around the image, the size (dotted box) can also be adjusted by using the trackball; to toggle between the two boxes use the right “mouse” button.
4. Start and stop data acquisition using
6. To store a still image press .
Fig.9 – Color Doppler acquisition window – Left Common Carotid Artery transverse view
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 9 of 12 Contrast Mode Contrast Mode imaging is used in conjunction with the MicroMarker Contrast Agents to enhance the visualization of blood flow down to the capillary level, and also to detect the expression of various biomarkers on the cell surface of endothelial cells.
1. In B-Mode imaging optimize the image for the target of interest to be imaged in Contrast Mode.
2. Press the Contrast button on the keyboard to start acquisition.
3. Start and stop data acquisition using
5. To store a cineloop press
6. To store a still image press . Note: Contrast Mode is also supported in 3D-Mode. To acquire Contrast-3D-Mode data, from Contrast Mode click 3D and set the acquisition parameters. To open the Contrast 3D Mode data in the 3D analysis window, users need to press the Load in 3D button.
Fig.10 – Loading Contrast 3D data in the 3D window
Fig.11 – Contrast Mode acquisition window – Non-Targeted MicroMarker Contrast Agent in a subcutaneous tumor on the hind limb
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 10 of 12
Data Review
1. To review the data. a. To review the study data press Study Managment b. To select a specific loop for review double click on the thumbnail image, or on the image label
2. To export still images or movies a. Open the desired cineloop b. If exporting a movie adjust the playback controls to the desired frames and playback speed; if exporting a picture select the appropriate frame
c. Click on Export d. Select the appropriate Export Type e. In options change the name of the file if desired and choose the appropriate File Type
f. The save location can also be chosen on the left side of the window
2. To save the study. a. Select Close from Study Managment to close the study. (Users will have the option of adding a new series to the study at later time points) b. To copy the study to an accessible drive, click Copy To c. To import a study into the software, for example on a workstation, open the software, click on Copy From, and select the study from the accessible drive
Data Analysis 1. To add measurements press
¾ Cardiac ¾ Abdominal ¾ Embryology ¾ Ophtalmology ¾ Vascular All these packages are available in all modes except Contrast Mode. Users can define custom measurements packages where they can select:
- package content and the display properties of the measurement (font, size, color) There is no limit for the number of measurements of the same type. The measurements report displays all the values, the
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 11 of 12 average for all measurements, and the standard deviation.
2. To add annotations right-click the image or press
Shutting Down the Vevo 2100
1. At the end of the session click Close Study. 2. Press the computer standby switch on the left side of the system to shutdown the software and turn off the computer.
3. Turn off the Vevo System by turning off the breaker at the back of the system.
Need help? Call us toll-free to 1-866-416-4636 or via email at [email protected]
Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 12 of 12