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Beginner's Guide to Vevo® 2100

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Beginner's Guide to Vevo® 2100 PN 12090 Beginner’s Guide to Vevo® 2100 December 2008 Beginner’s Guide to the Vevo® 2100 Rev. 1.4 This Beginner’s Guide to Vevo 2100 provides a quick overview of the Vevo 2100 imaging modes and provides key tips to allow users to start a study, acquire images, make measurements and export data. A more detailed step-by-step description of the system is available in the Vevo 2100 Operator Manual. Connect the transducer Attach the transducer that will be used during the imaging session to the connector on the front panel of the Vevo 2100. Start the Vevo 2100 1. Turn on the Vevo system using the breaker switch at the back of the system and then the computer by clicking the standby switch on the left side of the system. 2. Wait for the Windows Vista operating system to start-up. The system is configured by VisualSonics® to automatically log in. The Vevo 2100 software should automatically launch. Start the Vevo 1. When a scanhead is connected the transducer initialization window appears. Software 2. Select the appropriate application and click Initialize. Initialization sets up the appropriate preset parameters for the selected application and specific transducer. Fig.1 – Initialization Dialog 2. Once the transducer is initialized, the application opens in the Study Browser. The Study Browser window is used for sorting acquired data, for review and data export. Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Click the New button in the Study Browser to open the “New Study” window. In this window the user can start a brand new study or a new series; a series is a new session that could be part of an old study. Fig.2 – New Study dialog 3. Select one of the options. The application is going to open the Study Info window where the user needs to enter required information in order to start the study. The fields marked with yellow have to be filled in before the user can start imaging. Fig.3 – Study/Series Information 4. Once all the required information is entered press OK and the software automatically begins imaging in B-Mode. There are various imaging modes available, accessible by the mode buttons located on the keyboard Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 3 of 12 B-Mode B-Mode imaging is used to visualize anatomical structures in a 2D greyscale image. 1. Press B-Mode to start acquistion. The acquisition parameters panel is activated by default. Users can change the parameters as needed and can save a custom set by pressing Save Presets. 2. Press <Scan/Freeze> to start and stop data acquisition. 3. Use the Cine Loop Review knob to scroll through the acquired data, once you have frozen the current acquisition, or re- loaded an acquired loop. 4. To store a cineloop press <Cine Store>. The system can store a cineloop while actively scanning, or once the current acquisition has been frozen. 5. To store a single frame from a live acquisition or a frozen acquisition press <Frame Store>. 6. Users can simultaneously label and save a cine loop: by pressing Image Label, enter the Image name, and then press enter. Fig.4 – B-Mode acquisition window – left kidney in transverse view B-Mode is available on all Vevo 2100 systems, the following imaging modes are available as options to the Vevo 2100. You will only be able to use the options that have been purchased with your system. Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 4 of 12 M-Mode M-Mode imaging is used for visualizing and quantifying tissue movement over time, for example the motion of the myocardium over time. 1. In B-Mode acquisition, optimise the image for the area to be imaged in M-Mode. 2 Press the M-Mode button on the keyboard to display the M- Mode overlay. Adjust the position by moving the trackball, and size by clicking the SV Gate toggle up or down. Press the M- Mode button again or click the right mouse button. 3 Start and stop data acquisition using <Scan/Freeze>. New M-Mode image lines enter the image area from the right side and scroll across the window to the left in real-time. 4 Use the Cine Loop Review knob to scroll through the acquired data. 5 To store a data cineloop press <Cine Store>, this can be done in live scanning or when the current acquisition has been frozen; or use the Auto Image Label function (See B-Mode imaging Step . 6 To store a still frame image select <Frame Store>, again this can be done in live or frozen acquisition. Fig.5 – M-Mode acquisition window – Left Ventricle short axis view Fig. 5 – M-Mode Acquisition Window – Abdominal Aorta Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 5 of 12 Pulsed Wave (PW) PW Doppler imaging is used for visualizing and quantifying Doppler Mode blood flow through vessels and values in the heart. 1. In B-Mode acquisition, locate the region of interest for PW Doppler acquisition. Ensure that the angle between the direction of blood flow and the transmitted ultrasound beam is 60o or less. 2. Press the PW button on the keyboard. 3. Position and size the sample volume at the desired location for PW Doppler signal acquisition; use the trackball to change the sample volume position and the SV Gate toggle to change the size of the sample volume. 4. Adjust the PW Doppler angle using the Doppler Angle knob the yellow dotted line should be placed in line with the blood flow. If steering is needed to reduce the angle between blood flow and ultrasound beam use the Beam Angle toggle to steer the angle either to the right or left. 5. Press the right “mouse” button or the PW button again to switch to the PW Doppler acquisition window. 6. Start and stop data acquisition using <Scan/Freeze>. New Doppler lines enter the image area from the right side and scroll across the window to the left in real-time. The audio representation of the acquired Doppler data is heard through the system speakers, the volume can be adjusted using the rotary dial on the keyboard. 7. Use the Cine Loop Review knob to scroll through the acquired data. 8. To store a cineloop press <Cine Store> or use the Auto-Image Label function. (See B-Mode imaging Step 6) 9. To store a still image press <Frame Store>. Fig.6 – PW Doppler acquisition window – Mitral Valve apical four chamber view Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 6 of 12 3D-Mode 3D-Mode imaging is used to visualize an anatomical structure in three dimensions. Multiple 2D slices are acquired and compiled into a 3D volume. 3D-Mode imaging can be used for B-Mode Power Doppler Mode, and Contrast Mode Data. 1. Connect the 3D motor stage to the Vevo Rail System, attach the motor to the ball joint, where the scanhead clamp can also be attached. 2. Connect the transducer clamp to the 3D motor. 3. Mount the transducer in the clamp; the transducer imaging plane must be perpendicular to the axis along which the motor will move. 4. Press the <3D> button on the keyboard. The 3D motor initialization dialog will come up. Click okay and the motor will initialize. Ensure that the probe is not touching an animal and that nothing will abstruct it’s movement. 5. Optimize the image for acquisition, for example in B-Mode and determine the range the motor must move to cover the desired volume using the micromanipulators on the stage for the animal handling platform. 6. When the acquisition ends the application opens the 3D analysis window. If using Contrast Mode, the image is loaded into a 2D window frame which can then be loaded into 3D once all post processing steps are complete; this is done by clicking Load in 3D. 7. To store the data press <3D Store> or Auto-Save on Image Label. Fig.7 – 3D Mode analysis window – Left Ventricle using ECG and Respiration gating Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 7 of 12 Power Doppler Mode Power Doppler Mode imaging is used to visualize blood flow; generally Power Doppler is used to visualize flow in structures such as tumors or abdominal organs. 1. In B-Mode imaging optimize the image for the target of interest to be imaged in Power Doppler. 2. Press the Power button on the keyboard to start acquisition 3. Position and size the Power Box at the desired location for Power Doppler signal acquisition. The position (solid box) of the Power Box can be adjusted by moving the Trackball around the image, the size (dotted box) can also be adjusted by using the trackball; to toggle between the two boxes use the right “mouse” button. 4. Start and stop acquisition using <Scan/Freeze>. 5. To store a cineloop press <Cine Store> or the Auto-Save on Image Label. (see B-Mode imaging Step 6). 6. To store a still image press <Frame Store>. Note: Power Doppler Mode is also supported in 3D-Mode. To acquire Power-3D-Mode data, from Power Doppler Mode click 3D and adjust the 3D acqusition parameters. At the end of the acquisition the application switches to Power 3D analysis window. Fig.8 – Power Doppler acquisition window – Subcutaneous Tumor on hind limb Beginner’s Guide to the Vevo® 2100 Rev. 1.4 Page 8 of 12 Color Doppler Mode Color Dopper imaging is used to visualize blood flow and give additional information about the direction of flow.
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