beta-Galactosidase Cat. No.: 5155

Western blot analysis of (A) 5 and (B) 25 ng of b-gal with b-gal antibody at 1 μg/mL.

Specifications

HOST SPECIES: Chicken

SPECIES REACTIVITY: Bacteria

beta-gal antibody was raised against a 17 synthetic peptide from near the amino terminus of E. coli beta-gal. IMMUNOGEN:

The immunogen is located within amino acids 20 - 70 of beta-Galactosidase.

TESTED APPLICATIONS: ELISA, WB

APPLICATIONS: beta-gal antibody can be used for detection of beta-gal by Western blot at 1 μg/mL.

Properties

PURIFICATION: beta-Galactosidase Antibody is purified via peptide column.

CLONALITY: Polyclonal

ISOTYPE: IgY

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: beta-Galactosidase Antibody is supplied in PBS containing 0.02% sodium azide.

September 24, 2021 1 https://www.prosci-inc.com/beta-galactosidase-antibody-5155.html CONCENTRATION: 1 mg/mL

beta-Galactosidase antibody can be stored at 4˚C for three months and -20˚C, stable for STORAGE CONDITIONS: up to one year. As with all care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures.

Additional Info

OFFICIAL SYMBOL: lacZ

ALTERNATE NAMES: beta-Galactosidase Antibody: Beta-galactosidase, Lactase, Beta-gal

ACCESSION NO.: NP_752394

PROTEIN GI NO.: 26246355

GENE ID: 1036228

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

beta-Galactosidase Antibody: Beta-galactosidase (beta-gal), an inducible that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a BACKGROUND: product of the LacZ operon, and is a homo-tetrameric consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter in molecular biology to assess the efficiency of transfection.

REFERENCES: 1) Matthews BW. The structure of E. coli beta-galactosidase. C. R. Biol.2005; 328:549-56.

ANTIBODIES FOR RESEARCH USE ONLY.

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September 24, 2021 2 https://www.prosci-inc.com/beta-galactosidase-antibody-5155.html