beta-Galactosidase Antibody Cat. No.: 5155 Western blot analysis of (A) 5 and (B) 25 ng of b-gal with b-gal antibody at 1 μg/mL. Specifications HOST SPECIES: Chicken SPECIES REACTIVITY: Bacteria beta-gal antibody was raised against a 17 amino acid synthetic peptide from near the amino terminus of E. coli beta-gal. IMMUNOGEN: The immunogen is located within amino acids 20 - 70 of beta-Galactosidase. TESTED APPLICATIONS: ELISA, WB APPLICATIONS: beta-gal antibody can be used for detection of beta-gal by Western blot at 1 μg/mL. Properties PURIFICATION: beta-Galactosidase Antibody is affinity chromatography purified via peptide column. CLONALITY: Polyclonal ISOTYPE: IgY CONJUGATE: Unconjugated PHYSICAL STATE: Liquid BUFFER: beta-Galactosidase Antibody is supplied in PBS containing 0.02% sodium azide. September 24, 2021 1 https://www.prosci-inc.com/beta-galactosidase-antibody-5155.html CONCENTRATION: 1 mg/mL beta-Galactosidase antibody can be stored at 4˚C for three months and -20˚C, stable for STORAGE CONDITIONS: up to one year. As with all antibodies care should be taken to avoid repeated freeze thaw cycles. Antibodies should not be exposed to prolonged high temperatures. Additional Info OFFICIAL SYMBOL: lacZ ALTERNATE NAMES: beta-Galactosidase Antibody: Beta-galactosidase, Lactase, Beta-gal ACCESSION NO.: NP_752394 PROTEIN GI NO.: 26246355 GENE ID: 1036228 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References beta-Galactosidase Antibody: Beta-galactosidase (beta-gal), an inducible enzyme that catalyses the hydrolysis of lactose and other beta-galactosides into monosaccharides, is a BACKGROUND: product of the LacZ operon, and is a homo-tetrameric protein consisting of four identical subunits of approximately 114 kDa each. The enzyme is commonly used as a reporter gene in molecular biology to assess the efficiency of transfection. REFERENCES: 1) Matthews BW. The structure of E. coli beta-galactosidase. C. R. Biol.2005; 328:549-56. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 24, 2021 2 https://www.prosci-inc.com/beta-galactosidase-antibody-5155.html.