NAP1L4, Recombinant, Human, Aa1-375, His-Tag (Nucleosome Assembly Protein 1-Like 4)

NAP1L4, Recombinant, Human, aa1-375, His-Tag (Nucleosome assembly protein 1-like 4)

Catalog number 350102 Supplier United States Biological

NAP1L4 is a member of the nucleosome assembly protein (NAP) family which can interact with both core and linker histones. It can shuttle between the cytoplasm and nucleus, suggesting a role as a histone chaperone. This gene is one of several located near the imprinted gene domain of 11p15.5, an important tumor-suppressor gene region. Alterations in this region have been associated with the Beckwith-Wiedemann syndrome, Wilms tumor, rhabdomyosarcoma, adrenocortical carcinoma, and lung, ovarian, and breast cancer. Source Recombinant protein corresponding to aa1-375 of human NAP1L4, fused to His-tag at N-terminal expressed in E. coli. Molecular Weight ~45.2kD AA Sequence MGSSHHHHHH SSGLVPRGSH MGSMADHSFS DGVPSDSVEA AKNASNTEKL TDQVMQNPRV LAALQERLDN VPHTPSSYIE TLPKAVKRRI NALKQLQVRC AHIEAKFYEE VHDLERKYAA LYQPLFDKRR EFITGDVEPT DAESEWHSEN EEEEKLAGDM KSKVVVTEKA AATAEEPDPK GIPEFWFTIF RNVDMLSELV QEYDEPILKH LQDIKVKFSD PGQPMSFVLE FHFEPNDYFT NSVLTKTYKM KSEPDKADPF SFEGPEIVDC DGCTIDWKKG KNVTVKTIKK KQKHKGRGTV RTITKQVPNE SFFNFFNPLK ASGDGESLDE DSEFTLASDF EIGHFFRERI VPRAVLYFTG EAIEDDDNFE EGEEGEEEEL EGDEEGEDED DAEINPKV Storage and Stability May be stored at 4°C for short-term only. Aliquot to avoid repeated freezing and thawing. Store at -20°C. Aliquots are stable for 6 months after receipt at -20°C. For maximum recovery of product, centrifuge the original vial after thawing and prior to removing the cap. Further dilutions can be made in assay buffer. Formulation Supplied as a liquid in 20mM Tris-HCl, pH 8.0, 0.15M sodium chloride, 1mM DTT, 20% glycerol. Purity ~90% (SDS-PAGE) Grade Purified Storage -20°C MW 45.2 Antigen Modification Recombinant, E. coli Reference 1. Olsen J.V., et al. (2006) Cell. 127:635-648, 2. Nousiainen M., et al. (2006) Proc. Natl. Acad. Sci. u.S.A. 103:5391-5396.