Biomarker Responses in Fish from Frierfjord and Eidanger
View metadata, citation and similar papers at core.ac.uk brought to youCORE by provided by NIVA Open Access Archive REPORT SNO 4857-2004 Biomarker responses in fish from Frierfjord and Eidanger Frierfjorden Eidangerfjorden Kastebukta [ [ Kalvsundet Langesundsfjorden 3,5 3,0 2,5 2,0 [ Vesle1,5 Arøya female 1,0 0,5 0,0 -0,5 3,5 3,0 log (EROD) 2,5 2,0 1,5 male 1,0 0,5 N Langesundsbukta0,0 -0,5 E EF EF EF S 0246810Kilometers Contaminants in the Grenland fjords Biomarker responses in fish from Frierfjord and Eidanger Subproject 3 – biological effects NIVA 4857-2004 Preface The current report forms part of a 3-year project on the abiotic transport, bioaccumulation and effects of dioxins and dibenzofurans in Frierfjord and Eidanger. The main project was managed by Kristoffer Næs, NIVA, and was a collaboration between NIVA, IMR (Flødevigen) and NGI. NIVA was in charge of the activity described herein, which was performed in collaboration with IMR (collection of material, herring egg exposure). Oslo, 5. July, 2004 Ketil Hylland NIVA 4857-2004 Contents Summary 6 1. Introduction 7 2. Interpretation of biological effects 8 2.1 Baseline responses 8 2.2 Comparing populations 9 2.3 Correcting for other factors 10 3. Materials and methods 11 3.1 Collection and sampling 11 3.2 Calculation of condition and LSI 11 3.3 Sample treatment 11 3.4 Analysis of protein 11 3.5 Analysis of CYP1A protein 11 3.6 Analysis of EROD (CYP1A activity) 12 3.7 Analysis of GST activity 12 3.8 Analysis of glutathione reductase 12 3.9 Analysis of vitellogenin 12 3.10 Statistical analyses 12 4.
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