DOCSLIB.ORG

Germinability After Desiccation, Storage and Cryopreservation of Seeds from Endemic Encholirium Mart

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
Germinability After Desiccation, Storage and Cryopreservation of Seeds from Endemic Encholirium Mart Acta bot. bras. 21(4): 777-783. 2007 Germinability after desiccation, storage and cryopreservation of seeds from endemic Encholirium Mart. ex Schult. & Schult. f. and Dyckia Schult. & Schult. f. species (Bromeliaceae) Erika Tarré1, Bárbara Balzana Mendes Pires1, Ana Paula Mançano Guimarães1, Leonardo Alves Carneiro1, Rafaela Campostrini Forzza2 and Elisabeth Mansur1,3 Received: September 22, 2006. Accepted: February 9, 2007 RESUMO – (Germinabilidade de sementes de espécies endêmicas de Encholirium Mart. ex Schult. & Schult. f. e Dyckia Schult. & Schult. f. (Bromeliaceae) após dessecação, armazenamento e criopreservação). O armazenamento de sementes requer a determinação prévia das condições ótimas de temperatura e luz para a germinação, assim como da tolerância à dessecação e a baixas temperaturas. O objetivo deste trabalho foi o estudo do efeito da dessecação, armazenamento a baixa temperatura e criopreservação de seis espécies de Encholirium e duas de Dyckia, selecionadas de acordo com critérios de vulnerabilidade. A germinabilidade de sementes recém-coletadas variou entre 35 e 95%. As sementes apresentaram comportamento fotoblástico, uma vez que a presença de luz foi necessária para induzir a germinação ou aumentar sua eficiência. A dessecação não afetou significativamente a germinabilidade das sementes testadas, com exceção de E. heloisae e E. scrutor. Sementes dessecadas e armazenadas durante um ano a 4 e -20 °C não apresentaram alterações na germinabilidade, exceto em E. pedicellatum. A germinabilidade após congelamento em nitrogênio líquido foi maior que ou similar à obtida no controle, em todas as espécies estudadas. Entretanto, em E. pedicellatum a tolerância ao congelamento só foi obtida após dessecação das sementes a um conteúdo hídrico de 2,5%. Considerando a tolerância à dessecação e ao armazenamento em baixas temperaturas, as sementes estudadas podem ser classificadas como ortodoxas e conservadas ex situ. Palavras-chave: Cadeia do Espinhaço, conservação de sementes, criopreservação, germinação, sementes ortodoxas ABSTRACT – (Germinability after desiccation, storage and cryopreservation of seeds from endemic Encholirium Mart. ex Schult. & Schult. f. and Dyckia Schult. & Schult. f. species (Bromeliaceae)). Seed storage procedures require previous determination of optimal temperature and light conditions for germination, as well as of tolerance to desiccation and low temperatures. The aim of this paper was to study the effects of desiccation, storage at low temperatures and cryopreservation on the germinability of seeds of six Encholirium and two Dyckia species, which were selected according to vulnerability criteria. Initial germinability of newly harvested seeds varied from 35 to 95%. Seeds presented photoblastic behaviour since light was necessary to induce or increase germination. Except for E. heloisae and E. scrutor, desiccation did not affect significantly the germinability of tested seeds. Storage for one year at 4 and -20 °C did not affect the germinability of desiccated seeds, except for E. pedicellatum. Germinability after freezing in liquid nitrogen was higher than or similar to control seeds for all species. However, freezing tolerance of E. pedicellatum seeds was only achieved after desiccation to 2.5% moisture content. As regards tolerance to desiccation and to storage at low temperatures, the seeds studied here can be classified as orthodox and conserved ex situ. Key words: cryopreservation, Espinhaço mountain range, germination, orthodox seeds, seed conservation Introduction vegetation (Forzza & Wanderley 1998), in which the Bromeliaceae family plays an important part. Surveys Pitcairnioideae is one of the three subfamilies of of the area have demonstrated the existence of a high Bromeliaceae, including 16 genera that are represented diversity and level of endemism, especially among in Brazil by two endemic taxa, Dyckia and members of Pitcairnioideae. Thus, bromeliad species Encholirium (Forzza & Wanderley 1998). Great part occurring in this region are important for a better of the Brazilian species of this subfamily is understanding of genus delimitation and phylogenetic concentrated in Espinhaço mountain range (Minas lines (Giulietti et al. 1987). However, as a consequence Gerais, Brazil), a region characterized by rupestral field of several impacts, such as selective exploitation, fire 1 Universidade do Estado do Rio de Janeiro, Departamento de Biologia Celular e Genética, Laboratório de Micropropagação e Transformação de Plantas, Rua São Francisco Xavier 524 - PHLC, sala 505, 20550-013 Rio de Janeiro, RJ, Brasil 2 Instituto de Pesquisa Jardim Botânico do Rio de Janeiro, Rua Pacheco Leão 915, 22460-030 Rio de Janeiro, RJ, Brasil 3 Corresponding Author: [email protected] 778 Tarré, Pires, Guimarães, Carneiro, Forzza & Mansur: Germinability after desiccation, storage and cryopreservation... and devastation of habitats by urbanization, many cases problems such as abnormal germination or death endemic species of Espinhaço mountain range are by internal injuries have been reported (Vertucci 1989). seriously threatened, requiring protection by These problems are generally associated with seed conservation programs. characteristics such as size, moisture content and As in situ conservation of species with restricted chemical composition (Belletti et al. 1990). Thus, the endemism relies on conservation practices that are not effect of freezing must be analysed on each species always rigorously applied or respected, complementary before proposing cryopreservation as a conservation strategies by ex situ germplasm banks are method. recommended. Seed storage is the most used method In the present work, six endemic species of for ex situ conservation, since seeds are the natural Encholirium Mart. ex Schult. & Schult. f. and two of structures of plant reproduction and each one may Dyckia Schult. & Schult. f. occurring at Espinhaço represent a potentially unique and genetically different mountain range were selected according to vulnerability plant. In addition to being a cost-effective method, criteria. The list of endangered species of Minas Gerais sampling and storage of seeds from natural populations State (Mendonça & Lins 2000) includes E. heloisae are important to support restoration programs (Vasquéz- (L.B. Sm.) Forzza & Wand. as vulnerable to extinction, Yanes & Aréchiga 1996; Slageren 2003). Although the adopting the IUCN categories. E. pedicellatum (Mez.) rupestral field vegetation of Espinhaço mountain range Rauh and Dyckia ursina L.B. Sm. were considered has been studied for many years, there is lack of as critically endangered. E. reflexum Forzza & Wand., information about seed biology of native species E. magalhaesii L.B. Sm., E. subsecundum (Baker) (Madeira & Fernandes 1999). Mez., E. scrutor (L.B. Sm.) Rauh and D. sordida The evaluation of seed storage behaviour is the Baker were not included in the list, but are also exposed first step for the establishment of storage conditions, to risk of genetic erosion or even extinction (Forzza including information on temperature and light et al. 2003). Germination, desiccation tolerance, requirements for germination, desiccation tolerance and germinability after storage, and cryocapability of seeds freezing sensitivity. However, seed germination is one of these species were investigated to explore the of the less studied aspects of bromeliad reproduction, possibilities for their ex situ conservation. as optimal temperatures and light requirement for germination were only evaluated in about 50 of the Material and methods 2700 species of the family (Downs 1963; Mercier & Guerreiro Filho 1990). Seed material – Seed harvest was performed according Maintenance of seed viability after storage can to the dispersion periods of the chosen species. Seeds be achieved by adjusting the moisture content to a of Encholirium reflexum, E. magalhaesii, critical level and by reducing the temperature. Since E. subsecundum, E. scrutor and E. pedicellatum insufficient drying may result in less than maximum were harvested at Diamantina city and E. heloisae, longevity, and over-drying may reduce seed Dyckia sordida and D. ursina at Serra do Cipó physiological quality (Walters et al. 1998), the moisture National Park, Espinhaço mountain range, Minas Gerais level at which viability of seeds can be maintained after State, in May and July 2003. The region presents a long periods must be evaluated. mesotermic climate (CwB according to the Köppen Cryostorage techniques have an important system) with a dry season from May to August and a application for preserving endangered species (Touchell rainy season from September to April, with an average & Dixon 1994), allowing seeds to be stored for precipitation around 1350 mm. The average annual indefinite periods of time at ultra-low temperatures that temperature ranges between 25 and 30 °C in the reduce metabolic rates and deterioration. In these summer and 8 to 18 °C in the winter (Madeira & systems, the problems of traditional seed storage, such Fernandes 1999). as DNA damages, requirement for periodic evaluations, In order to obtain representative seed samples of viability control, risk of loss by disease and the local population, 10-12 fruits per plant were environmental problems are reduced or even eliminated collected from at least ten individuals. Fruits with (Benson et al. 1996; Moukadiri et al. 1999; Pita et al. opened capsules were considered as mature. Samples 1998). Although cryogenic storage has been carried consisted of
Load more

Recommended publications
  • Floristic and Ecological Characterization of Habitat Types on an Inselberg in Minas Gerais, Southeastern Brazil
    Acta Botanica Brasilica - 31(2): 199-211. April-June 2017. doi: 10.1590/0102-33062016abb0409 Floristic and ecological characterization of habitat types on an inselberg in Minas Gerais, southeastern Brazil Luiza F. A. de Paula1*, Nara F. O. Mota2, Pedro L. Viana2 and João R. Stehmann3 Received: November 21, 2016 Accepted: March 2, 2017 . ABSTRACT Inselbergs are granitic or gneissic rock outcrops, distributed mainly in tropical and subtropical regions. Th ey are considered terrestrial islands because of their strong spatial and ecological isolation, thus harboring a set of distinct plant communities that diff er from the surrounding matrix. In Brazil, inselbergs scattered in the Atlantic Forest contain unusually high levels of plant species richness and endemism. Th is study aimed to inventory species of vascular plants and to describe the main habitat types found on an inselberg located in the state of Minas Gerais, in southeastern Brazil. A total of 89 species of vascular plants were recorded (belonging to 37 families), of which six were new to science. Th e richest family was Bromeliaceae (10 spp.), followed by Cyperaceae (seven spp.), Orchidaceae and Poaceae (six spp. each). Life forms were distributed in diff erent proportions between habitats, which suggested distinct microenvironments on the inselberg. In general, habitats under similar environmental stress shared common species and life-form proportions. We argue that fl oristic inventories are still necessary for the development of conservation strategies and management of the unique vegetation on inselbergs in Brazil. Keywords: endemism, granitic and gneissic rock outcrops, life forms, terrestrial islands, vascular plants occurring on rock outcrops within the Atlantic Forest Introduction domain, 416 are endemic to these formations (Stehmann et al.
    [Show full text]
  • 16 Artigo Dyckia Racinae.Indd 1 17/12/2014 10:21:23 398 DORNELES, M.P.; OLIVEIRA, DE J.M.S
    Dyckia racinae L.B.Sm. (Bromeliaceae ): morphological description emphasizing the reproductive structures 397 Dyckia racinae L. B. Sm. (Bromeliaceae): morphological description emphasizing the reproductive structures 1 Mariane Paludette Dorneles2, João Marcelo Santos de Oliveira3 & Thais Scotti do Canto-Dorow 4 1 Parte da dissertação de Mestrado da primeira autora no Programa de Pós-graduação em Agrobiologia, Universidade Federal de Santa Maria. 2 Programa de Pós-graduação em Agrobiologia, Universidade Federal de Santa Maria. 3 Universidade Federal de Santa Maria, Centro de Ciências Naturais e Exatas, Departamento de Biologia, Programa de Pós-graduação em Agrobiologia, Av. Roraima s/n, CEP 97105-900, Santa Maria, RS, Brasil - [email protected] 4 Universidade Federal de Santa Maria, Centro de Ciências Naturais e Exatas, Departamento de Biologia, Av. Roraima s/n, CEP 97105-900, Santa Maria, RS, Brasil. Recebido em 20.III.2014. Aceito em 8.X.2014. ABSTRACT – This study presents an analysis of the external morphology and anatomy, especially of the micromorphology of reproductive organs that are important for characterizing Dyckia racinae L.B.Sm. The presence of a parietal U-shaped thickening in the endothecium and in the connective differ from other Dyckia species. Characteristics of pollen grains and ovules, indicated by micromorphology of the sporoderm and structure of the chalazal appendix, respectively, are similar to other species, and useful for characterizing the genus. Preferences for rocky soils, besides leaf characteristics and infl orescence structure, approximate D. racinae to D. cabrerae Smith & Reitz in the main dichotomous keys for the genus. Considering that Dyckia racinae is endemic in Rio Grande do Sul, and that the original description of the species was proposed based on a single cultivated individual, it is clear that the characteristics described in the present study, based on individual species analyzed in their natural environment, are important botanical contributions.
    [Show full text]
  • BROMELI ANA PUBLISHED by the NEW YORK BROMELIAD SOCIETY1 (Visit Our Website
    BROMELI ANA PUBLISHED BY THE NEW YORK BROMELIAD SOCIETY1 (visit our website www.nybromeliadsociety.org) November, 2014 Vol. 51, No. 9 THE WBC IN HAWAII - Updates and Corrections by Herb Plever My report of the World Conference in the October issue was silent about visiting a local grower. We were scheduled to visit Larry McGraw’s garden during our trip to Lyon Arboretum and Nu’uanu Pali overlook, but were advised that we had to skip the visit because our bus couldn’t make the steep turnaround on Lisa Vinzant’s unnamed Auction Neo. the narrow road up to the garden. (We were running There was a lot of suspense about the late.) beautiful, unnamed Neoregelia generously But I learned from the In Larry McGraw’s garden - what donated by Lisa Vinzant, but it had not yet been looks like Neo. ‘Fireball’ in the back, report in the East London Tillandsia streptophylla in the middle auctioned when I had to leave. Lisa had given the Bromeliad Society (South and Tillandsia xerographica in front. buyer the right to name the plant (subject to her Africa) Newsletter that approval). I have heard that the plant went for another bus did manage to visit Larry McGraw’s $600 but the purchaser likely believes that is a garden and the people were very impressed. The bargain for such an outstanding plant. The winner and adjacent photo is from that Newsletter. any name given the plant have not yet been We did not stay to the end of the Rare Plant confirmed. (See photo above.) Auction on Saturday night after the banquet, as we Two trees dominated the coastal landscape on had an early flight to Kona the next morning.
    [Show full text]
  • Bromeliads Bromeliads Are a Family of Plants (Bromeliaceae, the Pineapple Family) Native to Tropical North and South America
    A Horticulture Information article from the Wisconsin Master Gardener website, posted 19 March 2012 Bromeliads Bromeliads are a family of plants (Bromeliaceae, the pineapple family) native to tropical North and South America. Europeans fi rst found out about bromeliads on Columbus’ second trip to the New World in 1493, where the pineapple (Ananas sp.) was being cultivated by the Carib tribe in the West Indies. The commercial pineapple (Ananas comosus) is native to southern Brazil and Paraguay. After the colonization of the New World it was rapidly transported to all areas of the tropics, and now is widely grown in tropical and sub- tropical areas. The only A collection of bromeliads placed on a tree at Costa Flores, Costa Rica. bromeliad to occur north of the tropics is Spanish “moss” (Tillandsia usneoides). It is neither Spanish nor a moss, but an epiphytic bromeliad. It doesn’t look much like a typical Commercial pineapple, Ananas comosus, bromeliad, though, with its long scaly stems and reduced in the fi eld. fl owers. Bromeliads are monocots, many of which, like their grass relatives, have a special form of photosynthesis that uses a variation of the more usual biochemical pathways to allow them to use water more effi ciently. Even though they come from the tropics, this helps those that are epiphytes contend with life in the treetops where there is limited water and a real danger of drying out. There are about 2500 species Many bromeliads are tropical and several thousand hybrids epiphytes. and cultivars. Many have brightly colored leaves, fl owers or fruit, and range in size from moss-like species of Tillandsia to the enormous Puya raimondii from the Andes which produces a fl owering stem up to 15 feet tall.
    [Show full text]
  • Taxonomic Revision of the Chilean Puya Species (Puyoideae
    Taxonomic revision of the Chilean Puya species (Puyoideae, Bromeliaceae), with special notes on the Puya alpestris-Puya berteroniana species complex Author(s): Georg Zizka, Julio V. Schneider, Katharina Schulte and Patricio Novoa Source: Brittonia , 1 December 2013, Vol. 65, No. 4 (1 December 2013), pp. 387-407 Published by: Springer on behalf of the New York Botanical Garden Press Stable URL: https://www.jstor.org/stable/24692658 JSTOR is a not-for-profit service that helps scholars, researchers, and students discover, use, and build upon a wide range of content in a trusted digital archive. We use information technology and tools to increase productivity and facilitate new forms of scholarship. For more information about JSTOR, please contact [email protected]. Your use of the JSTOR archive indicates your acceptance of the Terms & Conditions of Use, available at https://about.jstor.org/terms New York Botanical Garden Press and Springer are collaborating with JSTOR to digitize, preserve and extend access to Brittonia This content downloaded from 146.244.165.8 on Sun, 13 Dec 2020 04:26:58 UTC All use subject to https://about.jstor.org/terms Taxonomic revision of the Chilean Puya species (Puyoideae, Bromeliaceae), with special notes on the Puya alpestris-Puya berteroniana species complex Georg Zizka1'2, Julio V. Schneider1'2, Katharina Schulte3, and Patricio Novoa4 1 Botanik und Molekulare Evolutionsforschung, Senckenberg Gesellschaft für Naturforschung and Johann Wolfgang Goethe-Universität, Senckenberganlage 25, 60325, Frankfurt am Main, Germany; e-mail: [email protected]; e-mail: [email protected] 2 Biodiversity and Climate Research Center (BIK-F), Senckenberganlage 25, 60325, Frankfurt am Main, Germany 3 Australian Tropical Herbarium and Tropical Biodiversity and Climate Change Centre, James Cook University, PO Box 6811, Caims, QLD 4870, Australia; e-mail: [email protected] 4 Jardin Botânico Nacional, Camino El Olivar 305, El Salto, Vina del Mar, Chile Abstract.
    [Show full text]
  • Water Relations of Bromeliaceae in Their Evolutionary Context
    View metadata, citation and similar papers at core.ac.uk brought to you by CORE provided by Apollo Botanical Journal of the Linnean Society, 2016, 181, 415–440. With 2 figures Think tank: water relations of Bromeliaceae in their evolutionary context JAMIE MALES* Department of Plant Sciences, University of Cambridge, Downing Street, Cambridge CB2 3EA, UK Received 31 July 2015; revised 28 February 2016; accepted for publication 1 March 2016 Water relations represent a pivotal nexus in plant biology due to the multiplicity of functions affected by water status. Hydraulic properties of plant parts are therefore likely to be relevant to evolutionary trends in many taxa. Bromeliaceae encompass a wealth of morphological, physiological and ecological variations and the geographical and bioclimatic range of the family is also extensive. The diversification of bromeliad lineages is known to be correlated with the origins of a suite of key innovations, many of which relate directly or indirectly to water relations. However, little information is known regarding the role of change in morphoanatomical and hydraulic traits in the evolutionary origins of the classical ecophysiological functional types in Bromeliaceae or how this role relates to the diversification of specific lineages. In this paper, I present a synthesis of the current knowledge on bromeliad water relations and a qualitative model of the evolution of relevant traits in the context of the functional types. I use this model to introduce a manifesto for a new research programme on the integrative biology and evolution of bromeliad water-use strategies. The need for a wide-ranging survey of morphoanatomical and hydraulic traits across Bromeliaceae is stressed, as this would provide extensive insight into structure– function relationships of relevance to the evolutionary history of bromeliads and, more generally, to the evolutionary physiology of flowering plants.
    [Show full text]
  • Vee's Crypt House by Ray Clark
    Vol 42 Number 1 January, February & March 2018 PUBLISHED BY: Editor- Derek Butcher. Assist Editor – Bev Masters Born 1977 and still offsetting!' COMMITTEE MEMBERS President: Adam Bodzioch 58 Cromer Parade Millswood 5034 Ph: 0447755022 Secretary: Bev Masters 6 Eric Street, Plympton 5038 Ph: 83514876 Vice president: Peter Hall Treasurer: Trudy Hollinshead Committee: Penny Seekamp Julie Batty Dave Batty Sue Sckrabei Jeff Hollinshead Kallam Sharman Pam Nelson Ian Cook Life members: Margaret Butcher, Derek Butcher, : Len Colgan, Adam Bodzioch : Bill Treloar Email address: Meetings Venue: Secretary – [email protected] Maltese Cultural Centre, Web site: http://www.bromeliad.org.au 6 Jeanes Street, Cultivar Register http://botu07.bio.uu.nl/bcg/bcr/index.php Beverley List for species names http://botu07.bio.uu.nl/bcg/taxonList.php http://botu07.bio.uu.nl/brom-l/ altern site http://imperialis.com.br/ FOLLOW US ON FACEBOOK Pots, Labels & Hangers - Small quantities available all meetings. Time: 2.00pm. Second Sunday of st For special orders/ larger quantities call Ron Masters on 83514876 each month. Exceptions –1 Sunday in March, May, June & 3rd Sunday September. - or unless advised otherwise (see dates below). No meeting in December VISITORS & NEW MEMBERS WELCOME. B. Obi-Wan (Photo J. Batty) MEETING & SALES 2018 DATES 08/4/2018 (Safety in the garden-Ray Clark), 06/5/2018- 1st SUNDAY (Workshop- pups, soils etc), 3/6/2018-1st SUNDAY (Guest speaker)- Len Colgan 8/7/2018 (Challenges growing Brom’s), 12/8/2018(Winter brag) 16/9/2018-3rd SUNDAY (Plants from Interstate), 14/10/2018 (to be advised), 27/10/2018 & 28/10/2018 Show & Sales, 11/11/2018 -130PM start, pup exchange, special afternoon tea – bring a plate of finger food to share, plant auction.
    [Show full text]
  • Central Spine
    C ENTRAL S PINE Newsletter of the CENTRAL ARIZONA CACTUS AND SUCCULENT SOCIETY FEBRUARY, 2001 Our April show approaches fast. New show FROM YOUR PRESIDENT— schedules will be available at the February February 7 is considered the historical last frost meeting, as will be the entry cards. Please register day for Phoenix. We had a scare the night of for the show at the February meeting, if you can. February 8 when lows in the upper 20s were If you cannot be there, let me know so you can predicted but didn't materialize. Now, it seems, be registered and receive cards and a show winter is over. I have been watering some of my schedule. Bring in show plants April 4, plants through the winter on warm days, Wednesday, from noon until 7 p.m. especially Baja California and winter-rain Steve Southwell of RSVP Nurseries in San Jose, Andean cacti (Copiapoa, Echinopsis, Lobivia, CA, was to speak to us this month. We just some Mammillaria, Neoporteria and Neochilenia, learned that he has been hospitalized and will be Tephrocactus, Trichocereus.) Mammillarias have unable to travel for some time. Our thoughts are been blooming since late November, and with him and his wife, Rowena. Ancistrocactus, Astrophytum, Frailea, Neoporteria, Our speaker this month will be James Pickering and Turbinicarpus are all in bud or bloom now. of Tucson. James will speak on “The Virtues of If we have a few days in a row of warmer days Growing Discocactus in Small Spaces.” He has a and nights I'm going to water everything except Web page at http://www.brazilcacticult.org the plants needing real heat for growth.
    [Show full text]
  • Post-Seminal Development and Cryopreservation of Endemic Or Endangered Bromeliads
    An Acad Bras Cienc (2021) 93(1): e20191133 DOI 10.1590/0001-3765202120191133 Anais da Academia Brasileira de Ciências | Annals of the Brazilian Academy of Sciences Printed ISSN 0001-3765 I Online ISSN 1678-2690 www.scielo.br/aabc | www.fb.com/aabcjournal CROP SCIENCES Post-seminal development and cryopreservation Running title: POST-SEMINAL of endemic or endangered bromeliads DEVELOPMENT AND CRYOPRESERVATION OF BROMELIADS SIMONE S.S. SILVA, EVERTON H. SOUZA, FERNANDA V.D. SOUZA, DANIELA A.S. MAX, MONICA L. ROSSI & MARIA A.P.C. COSTA Academy Section: CROP Abstract: Vriesea bahiana, Hohenbergia castellanosii and Encholirium spectabile are endemic Brazilian species that are considered endemic or endangered. Development SCIENCES of strategies to conserve these species is important to prevent irreversible genetic erosion. The objective of this study was to evaluate the post-seminal development and seed cryopreservation of three endemic or in danger of extinction bromeliad species in e20191133 Brazil, to obtain a protocol that can safeguard the genetic variability of these species. In the seed cryopreservation assay, we evaluated fi ve desiccation periods. The seeds in the cryotubes were taken from the desiccator and immediately plunged into liquid 93 nitrogen. For the analysis of post-seminal development, seeds in different germination (1) stages were collected and evaluated by light and scanning electron microscopy. Vriesea 93(1) bahiana seeds frozen in liquid nitrogen presented almost 100% germination, indicating dormancy break of this species. Vriesea bahiana can be cryopreserved with 5.9% water DOI content after being dried for 24 hours. Hohenbergia castellanosii and E. spectabile 10.1590/0001-3765202120191133 seeds did not need to be desiccated before being cryopreserved.
    [Show full text]
  • Adaptive Radiation, Correlated and Contingent Evolution, and Net Species Diversification in Bromeliaceae
    Molecular Phylogenetics and Evolution 71 (2014) 55–78 Contents lists available at ScienceDirect Molecular Phylogenetics and Evolution journal homepage: www.elsevier.com/locate/ympev Adaptive radiation, correlated and contingent evolution, and net species diversification in Bromeliaceae Thomas J. Givnish a,*, Michael H.J. Barfuss b, Benjamin Van Ee c, Ricarda Riina d, Katharina Schulte e,f, Ralf Horres g, Philip A. Gonsiska a, Rachel S. Jabaily h, Darren M. Crayn f, J. Andrew C. Smith i, Klaus Winter j, Gregory K. Brown k, Timothy M. Evans l, Bruce K. Holst m, Harry Luther n, Walter Till b, Georg Zizka e, Paul E. Berry o, Kenneth J. Sytsma a a Department of Botany, University of Wisconsin-Madison, Madison, WI 53706, USA b Department of Systematic and Evolutionary Botany, Faculty of Life Sciences, University of Vienna, Vienna A-1030, Austria c School of Natural Sciences, Black Hills State University, Spearfish, SD 57799, USA d Real Jardín Botánico, CSIC, Plaza de Murillo 2, Madrid 28014, Spain e Department of Botany and Molecular Evolution, Research Institute Senckenberg and J.W. Goethe University, Frankfurt am Main D-60325, Germany f Australian Tropical Herbarium, James Cook University, Cairns, QLD 4878, Australia g GenXPro, Frankfurt am Main 60438, Germany h Department of Biology, Rhodes College, Memphis, TN 38112, USA i Department of Plant Sciences, University of Oxford, Oxford OX1 3RB, United Kingdom j Smithsonian Tropical Research Institute, Balboa, Ancon, Republic of Panama k Department of Botany, University of Wyoming, Laramie, WY 82071, USA l Department of Biology, Grand Valley State University, Allendale, MI 49401, USA m Marie Selby Botanical Gardens, Sarasota, FL 34236, USA n Gardens By The Bay, National Parks Board Headquarters, Singapore 259569, Singapore o Department of Ecology and Evolutionary Biology, University of Michigan, Ann Arbor, MI 48109, USA article info abstract Article history: We present an integrative model predicting associations among epiphytism, the tank habit, entangling Received 22 May 2013 seeds, C3 vs.
    [Show full text]
  • Dyckia Tubifilamentosa (Pitcairnioideae-Bromeliaceae): a New Species from Northeastern Brazil)
    Hoehnea 41(2): 315-319, 2 fig., 2014 Nota científica / Scientific note Dyckia tubifilamentosa(Pitcairnioideae-Bromeliaceae): a new species from Northeastern Brazil Maria das Graças Lapa Wanderley1,4, Gardene Maria de Sousa2 and Marccus Alves3 Received: 27.03.2014; accepted: 30.05.2014 ABSTRACT - (Dyckia tubifilamentosa (Pitcairnioideae-Bromeliaceae): a new species from Northeastern Brazil). Dyckia tubifilamentosa Wand. & G. Sousa is described and illustrated. It differs from the other species of the genus Dyckia by several distinctive characters, namely: the ovoid shape of the flower buds, the long and exserted staminal tube formed from completely connate white filaments, the anther initially connivent and then divergent, along with the fruit with partially persistent perianth. Dyckia tubifilamentosa is found in some areas of the semiarid region of Northeastern Brazil (Piauí State), growing in the Caatinga and transitional Caatinga-Cerrado biomes. Keywords: Caatinga, neotropics, semiarid RESUMO - (Dyckia tubifilamentosa (Pitcairnioideae-Bromeliaceae): uma nova espécie do Nordeste do Brasil). Dyckia tubifilamentosa Wand. & G. Sousa é aqui descrita e ilustrada. A espécie se distingue das demais do gênero Dyckia pelo botão floral ovoide; tubo estaminal exserto e esbranquiçado, formado pelos filetes completamente conados; anteras coniventes passando a divergentes e fruto com restos de perianto. Dyckia tubifilamentosa é conhecida de algumas áreas da região semiárida do Nordeste do Brasil (estado do Piauí), crescendo na Caatinga e áreas de
    [Show full text]
  • PHYLOGENY, ADAPTIVE RADIATION, and HISTORICAL BIOGEOGRAPHY of BROMELIACEAE INFERRED from Ndhf SEQUENCE DATA
    Aliso 23, pp. 3–26 ᭧ 2007, Rancho Santa Ana Botanic Garden PHYLOGENY, ADAPTIVE RADIATION, AND HISTORICAL BIOGEOGRAPHY OF BROMELIACEAE INFERRED FROM ndhF SEQUENCE DATA THOMAS J. GIVNISH,1 KENDRA C. MILLAM,PAUL E. BERRY, AND KENNETH J. SYTSMA Department of Botany, University of Wisconsin, Madison, Wisconsin 53706, USA 1Corresponding author ([email protected]) ABSTRACT Cladistic analysis of ndhF sequences identifies eight major bromeliad clades arranged in ladderlike fashion. The traditional subfamilies Tillandsioideae and Bromelioideae are monophyletic, but Pitcair- nioideae are paraphyletic, requiring the description of four new subfamilies, recircumscription of Pit- cairnioideae and Navioideae, the sinking of Ayensua, and description of the new genus Sequencia. Brocchinioideae are basalmost, followed by Lindmanioideae, both restricted to the Guayana Shield. Next is an unresolved trichotomy involving Hechtioideae from Central America, Tillandsioideae, and the remaining bromeliads in subfamilies Navioideae, Pitcairnioideae, Puyoideae, and Bromelioideae. Bromeliads arose as C3 terrestrial plants on moist infertile sites in the Guayana Shield roughly 70 Mya, spread centripetally in the New World, and reached tropical West Africa (Pitcairnia feliciana) via long-distance dispersal about 10 Mya. Modern lineages began to diverge from each other 19 Mya and invaded drier areas in Central and South America beginning 15 Mya, coincident with a major adaptive radiation involving the repeated evolution of epiphytism, CAM photosynthesis, impounding leaves, several features of leaf/trichome anatomy, and accelerated diversification at the generic level. This ‘‘bromeliad revolution’’ occurred after the uplift of the northern Andes and shift of the Amazon to its present course. Epiphytism may have accelerated speciation by increasing ability to colonize along the length of the Andes, while favoring the occupation of a cloud-forest landscape frequently dissected by drier valleys.
    [Show full text]