Pepperspray, CS, & Other 'Less-Lethal' Weapons
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CS Gas (2-Chlorobenzylidene Malononitrole) General Information
CS Gas (2-chlorobenzylidene malononitrole) General Information Key Points 2-chlorobenzylidene malononitrole or “CS” is a white crystalline solid when pure it is used by UK police forces as a temporary incapacitant spray when released, CS gas will disperse into the atmosphere in a matter of minutes exposure to CS gas may cause intense tear production, eye pain, chest tightness, coughing and sneezing skin contact will lead to burning and stinging with possible redness and blistering treatment is rarely needed because people usually recover within 15-20 minutes physical injury to the eyes may occur if CS gas is sprayed directly into them at a very close range PHE publications gateway number: 2014790 Published: October 2018 Compendium of Chemical Hazards: CS Gas (2-chlorobenzylidene malononitrole) Public Health Questions What is CS gas and what is it used for? 2-chlorobenzylidene malononitrole or “CS” is a white crystalline solid when pure. The substance causes irritation in humans and is used by UK police forces as a temporary incapacitant spray to subdue persons who pose a risk to themselves and/or the police officers and others in the vicinity. It may be dispersed in a smoke cloud or dissolved into liquid (e.g. the solvent methyl iso-butyl ketone) to be used as a spray. The CS spray carried by some UK police forces contains 5% 2-chlorobenzylidene malononitrole. It is also used by the military in training operations and for testing gas masks. These substances may be referred to as CS or tear spray/gas (not to be confused with PAVA spray which is also be referred to as tear spray/gas). -
TRPM8 Activation by Menthol, Icilin, and Cold Is Differenially Modulated by Intracellular Ph
5364 • The Journal of Neuroscience, June 9, 2004 • 24(23):5364–5369 Cellular/Molecular TRPM8 Activation by Menthol, Icilin, and Cold Is Differentially Modulated by Intracellular pH David A. Andersson, Henry W. N. Chase, and Stuart Bevan Novartis Institute for Medical Sciences, London WC1E 6BN, United Kingdom TRPM8 is a nonselective cation channel activated by cold and the cooling compounds menthol and icilin (Peier et al., 2002). Here, we have used electrophysiology and the calcium-sensitive dye Fura-2 to study the effect of pH and interactions between temperature, pH, and the two chemical agonists menthol and icilin on TRPM8 expressed in Chinese hamster ovary cells. Menthol, icilin, and cold all evoked 2ϩ ϩ stimulus-dependent [Ca ]i responses in standard physiological solutions of pH 7.3. Increasing the extracellular [H ] from pH 7.3 to approximately pH 6 abolished responses to icilin and cold stimulation but did not affect responses to menthol. Icilin concentration– response curves were significantly shifted to the right when pH was lowered from 7.3 to 6.9, whereas those with menthol were unaltered in solutions of pH 6.1. When cells were exposed to solutions in the range of pH 8.1–6.5, the temperature threshold for activation was elevatedathigherpHanddepressedatlowerpH.Superfusingcellswithalowsubactivatingconcentrationoficilinormentholelevatedthe 2ϩ threshold for cold activation at pH 7.4, but cooling failed to evoke [Ca ]i responses at pH 6 in the presence of either agonist. In voltage-clamp experiments in which the intracellular pH was buffered to different levels, acidification reduced the current amplitude of icilin responses and shifted the threshold for cold activation to lower values with half-maximal inhibition at pH 7.2 and pH 7.6. -
Camp Delta Standard Operating Procedures (SOP)
UNCLASSIFIED//FOR OFFICIAL USE ONLY Camp Delta Standard Operating Procedures (SOP) Headquarters, Joint Task Force - Guantanamo (JTF-GTMO) Guantanamo Bay, Cuba 28 March 2003 UNCLASSIFIED//FOR OFFICIAL USE ONLY UNCLASSIFIED//FOR OFFICIAL USE ONLY Intentionally Left Blank Camp Delta SOP 28 March 2003 UNCLASSIFIED//FOR OFFICIAL USE ONLY UNCLASSIFIED//FOR OFFICIAL USE ONLY Headquarters Joint Detention Operations Group (JDOG) Joint Task Force Guantanamo (JTF-GTMO) Guantanamo Bay, Cuba Effective 28 March 2003 28 March 2003 Camp Delta Standard Operating Procedures (SOP) By Order of the Commander procedures established herein apply to Supplementation. the services and agencies that Supplementation of this SOP and function in, and support thereof, establishment of command or local GEOFFREY D. MILLER detainee operations at JTF-GTMO in forms is prohibited without prior Major General, United States Army general and Camp Delta specifically. approval of the JDOG Commander, JTF-GTMO Applicability. This is a multi- Commander. service, multi-agency set of standard Suggested Improvements. Users Official: operating procedures. It applies to the are invited to send comments and Army, Navy, Air Force, Marine suggested improvements through ADOLPH MCQUEEN Corps, and Coast Guard as well as their chain of command to the Colonel, Military Police United States Government JDOG S3 for proper consideration Commander, JDOG organizations and international non- and staffing. governmental organizations operating Interim Changes. Policies and inside Camp Delta 1 - 3. procedures will be reviewed every History. This is a revised update to Proponent and exception authority. 120 days. Any interim changes the Joint Task Force Guantanamo The proponent of this set of operating will be signed by the JDOG (JTF-GTMO) standard operating procedures and special orders is the Commander and posted to the front procedures (SOP) for Camp Delta. -
Regulation of TRP Channels by Steroids
General and Comparative Endocrinology xxx (2014) xxx–xxx Contents lists available at ScienceDirect General and Comparative Endocrinology journal homepage: www.elsevier.com/locate/ygcen Review Regulation of TRP channels by steroids: Implications in physiology and diseases ⇑ Ashutosh Kumar, Shikha Kumari, Rakesh Kumar Majhi, Nirlipta Swain, Manoj Yadav, Chandan Goswami School of Biology, National Institute of Science Education and Research, Sachivalaya Marg, Bhubaneswar, Orissa 751005, India article info abstract Article history: While effects of different steroids on the gene expression and regulation are well established, it is proven Available online xxxx that steroids can also exert rapid non-genomic actions in several tissues and cells. In most cases, these non-genomic rapid effects of steroids are actually due to intracellular mobilization of Ca2+- and other ions Keywords: suggesting that Ca2+ channels are involved in such effects. Transient Receptor Potential (TRP) ion TRP channels channels or TRPs are the largest group of non-selective and polymodal ion channels which cause Ca2+- Steroids influx in response to different physical and chemical stimuli. While non-genomic actions of different Non-genomic action of steroids steroids on different ion channels have been established to some extent, involvement of TRPs in such Ca2+-influx functions is largely unexplored. In this review, we critically analyze the literature and summarize how Expression different steroids as well as their metabolic precursors and derivatives can exert non-genomic effects by acting on different TRPs qualitatively and/or quantitatively. Such effects have physiological repercus- sion on systems such as in sperm cells, immune cells, bone cells, neuronal cells and many others. -
Snapshot: Mammalian TRP Channels David E
SnapShot: Mammalian TRP Channels David E. Clapham HHMI, Children’s Hospital, Department of Neurobiology, Harvard Medical School, Boston, MA 02115, USA TRP Activators Inhibitors Putative Interacting Proteins Proposed Functions Activation potentiated by PLC pathways Gd, La TRPC4, TRPC5, calmodulin, TRPC3, Homodimer is a purported stretch-sensitive ion channel; form C1 TRPP1, IP3Rs, caveolin-1, PMCA heteromeric ion channels with TRPC4 or TRPC5 in neurons -/- Pheromone receptor mechanism? Calmodulin, IP3R3, Enkurin, TRPC6 TRPC2 mice respond abnormally to urine-based olfactory C2 cues; pheromone sensing 2+ Diacylglycerol, [Ca ]I, activation potentiated BTP2, flufenamate, Gd, La TRPC1, calmodulin, PLCβ, PLCγ, IP3R, Potential role in vasoregulation and airway regulation C3 by PLC pathways RyR, SERCA, caveolin-1, αSNAP, NCX1 La (100 µM), calmidazolium, activation [Ca2+] , 2-APB, niflumic acid, TRPC1, TRPC5, calmodulin, PLCβ, TRPC4-/- mice have abnormalities in endothelial-based vessel C4 i potentiated by PLC pathways DIDS, La (mM) NHERF1, IP3R permeability La (100 µM), activation potentiated by PLC 2-APB, flufenamate, La (mM) TRPC1, TRPC4, calmodulin, PLCβ, No phenotype yet reported in TRPC5-/- mice; potentially C5 pathways, nitric oxide NHERF1/2, ZO-1, IP3R regulates growth cones and neurite extension 2+ Diacylglycerol, [Ca ]I, 20-HETE, activation 2-APB, amiloride, Cd, La, Gd Calmodulin, TRPC3, TRPC7, FKBP12 Missense mutation in human focal segmental glomerulo- C6 potentiated by PLC pathways sclerosis (FSGS); abnormal vasoregulation in TRPC6-/- -
Pepper Spray: What Do We Have to Expect?
Pepper Spray: What Do We Have to Expect? Assoc. Prof. Mehmet Akif KARAMERCAN, MD Gazi University School of Medicine Department of Emergency Medicine Presentation Plan • History • Pepper Spray • Tear Gas • Symptoms • Medical Treatment • If you are the victim ??? History • PEPPER SPRAY ▫ OC (oleoresin of capsicum) (Most Commonly Used Compound) • TEAR GAS ▫ CN (chloroacetophenone) (German scientists 1870 World War I and II) ▫ CS (orthochlorobenzalmalononitrile) (US Army adopted in 1959) ▫ CR (dibenzoxazepine) (British Ministry of Defence 1950-1960) History of Pepper Spray • Red Chili Pepper was being used for self defense in ancient India - China - Japan (Ninjas). ▫ Throw it at the faces of their enemies, opponents, or intruders. • Japan Tukagawa Empire police used a weapon called the "metsubishi." • Accepted as a weapon ▫ incapacitate a person temporarily. • Pepper as a weapon 14th and 15th century for slavery rampant and became a popular method for torturing people (criminals, slaves). History of Pepper Spray • 1980's The USA Postal Workers started using pepper sprays against dogs, bears and other pets and became a legalized non-lethal weapon ▫ Pepper spray is also known as oleoresin of capsicum (OC) spray • The FBI in 1987 endorse it as an official chemical agent and it took 4 years it could be legally accepted by law enforcement agency. Pepper Spray • The active ingredient in pepper spray is capsaicin, which is a chemical derived from the fruit of plants of chilis. • Extraction of Oleoresin Capsicum from peppers ▫ capsicum to be finely ground, capsaicin is then extracted using an organic solvent (ethanol). The solvent is then evaporated, remaining waxlike resin is the Oleoresin Capsicum • Propylene Glycol is used to suspend the OC in water, pressurized to make it aerosol in Pepper Spray. -
Transient Receptor Potential Channels and Metabolism
Molecules and Cells Minireview Transient Receptor Potential Channels and Metabolism Subash Dhakal and Youngseok Lee* Department of Bio and Fermentation Convergence Technology, Kookmin University, BK21 PLUS Project, Seoul 02707, Korea *Correspondence: [email protected] https://doi.org/10.14348/molcells.2019.0007 www.molcells.org Transient receptor potential (TRP) channels are nonselective Montell, 2007). These cationic channels were first charac- cationic channels, conserved among flies to humans. Most terized in the vinegar fly, Drosophila melanogaster. While TRP channels have well known functions in chemosensation, a visual mechanism using forward genetic screening was thermosensation, and mechanosensation. In addition to being studied, a mutant fly showed a transient response to being sensing environmental changes, many TRP channels constant light instead of the continuous electroretinogram are also internal sensors that help maintain homeostasis. response recorded in the wild type (Cosens and Manning, Recent improvements to analytical methods for genomics 1969). Therefore, the mutant was named as transient recep- and metabolomics allow us to investigate these channels tor potential (trp). In the beginning, researchers had spent in both mutant animals and humans. In this review, we two decades discovering the trp locus with the germ-line discuss three aspects of TRP channels, which are their role transformation of the genomic region (Montell and Rubin, in metabolism, their functional characteristics, and their 1989). Using a detailed structural permeation property anal- role in metabolic syndrome. First, we introduce each TRP ysis in light-induced current, the TRP channel was confirmed channel superfamily and their particular roles in metabolism. as a six transmembrane domain protein, bearing a structural Second, we provide evidence for which metabolites TRP resemblance to a calcium-permeable cation channel (Mon- channels affect, such as lipids or glucose. -
Half Way Down the Trail to Hell
Half Way Down The Trail To Hell A Wartime Remembrance in Three Parts By Stephen E. Kirkland i Prologue “The danger, being around veterans, the memories are so selective and so heroic that you’ve got to be careful talking to a guy like me.” George Herbert Walker Bush A while back I discovered the Library of Congress is conducting a program called The Veterans History Project. The mission of the project is the collection and preservation of veteran’s wartime recollections and documents before they are lost forever. Like many wartime veterans, I concentrated on getting on with my life. I needed a job that would allow me to marry, buy a house and raise a family. I didn’t feel anything I had experienced in Vietnam would contribute to these goals, and I felt that, for the most part, people who hadn’t served didn’t know or even care what I had seen or done. The country’s attitude was different than with the Gulf War veterans and I deflected the few inquires that were made, especially the ones that contained the words “Did ya’ kill anybody?” I was too busy dealing with the present to spend a lot of time staring into the past. Now, almost four decades after returning home, the time has come to look back and try to recreate a piece of personal history, albeit history filtered though my water colored memories. I’ve relied on a number of sources for this remembrance, not the least of which were letters that I wrote home. -
Trpa1) Activity by Cdk5
MODULATION OF TRANSIENT RECEPTOR POTENTIAL CATION CHANNEL, SUBFAMILY A, MEMBER 1 (TRPA1) ACTIVITY BY CDK5 A dissertation submitted to Kent State University in partial fulfillment of the requirements for the degree of Doctor of Philosophy by Michael A. Sulak December 2011 Dissertation written by Michael A. Sulak B.S., Cleveland State University, 2002 Ph.D., Kent State University, 2011 Approved by _________________, Chair, Doctoral Dissertation Committee Dr. Derek S. Damron _________________, Member, Doctoral Dissertation Committee Dr. Robert V. Dorman _________________, Member, Doctoral Dissertation Committee Dr. Ernest J. Freeman _________________, Member, Doctoral Dissertation Committee Dr. Ian N. Bratz _________________, Graduate Faculty Representative Dr. Bansidhar Datta Accepted by _________________, Director, School of Biomedical Sciences Dr. Robert V. Dorman _________________, Dean, College of Arts and Sciences Dr. John R. D. Stalvey ii TABLE OF CONTENTS LIST OF FIGURES ............................................................................................... iv LIST OF TABLES ............................................................................................... vi DEDICATION ...................................................................................................... vii ACKNOWLEDGEMENTS .................................................................................. viii CHAPTER 1: Introduction .................................................................................. 1 Hypothesis and Project Rationale -
A Study for Health Hazard Evaluation of Methylene Chloride Evaporated from the Tear Gas Mixture
Saf Health Work 2010;1:98-101 | DOI:10.5491/SHAW.2010.1.1.98 pISSN : 2093-7911 eISSN : 2093-7997 Case Report A Study for Health Hazard Evaluation of Methylene Chloride Evaporated from the Tear Gas Mixture Seung-Hyun PARK, Eun-Kyo CHUNG, Gwang-Yong YI, Kwang-Jae CHUNG, Jung-Ah SHIN and In-Seop LEE Department of Occupational Environment Research, Occupational Safety and Health Research Institute, KOSHA, Incheon, Korea This study explored the health hazard of those exposed to methylene chloride by assessing its atmospheric concentration when a tear gas mixture was aerially dispersed. The concentration of methylene chloride ranged from 311.1–980.3 ppm (geometric mean, 555.8 ppm), 30 seconds after the dispersion started. However, the concentration fell rapidly to below 10 ppm after dispersion was completed. The concentration during the dispersion did not surpass the National Institute for Occupational Safety and Health ‘immediately dangerous to life or health’ value of 2,300 ppm, but did exceed the American Conference of Governmental Industrial Hygienists excursion limit of 250 ppm. Since methylene chloride is highly volatile (vapor pressure, 349 mmHg at 20oC), the post- dispersion atmospheric concentration can rise instantaneously. Moreover, the o-chlorobenzylidenemalononitrile formulation of tear gas (CS gas) is an acute upper respiratory tract irritant. Therefore, tear gas mixtures should be handled with delicate care. Key Words: Methylene chloride, CS tear gas, Tear gas mixture, Health hazard evaluation Introduction Despite this long history of use, CS gas has recently come under greater scrutiny since it can be applied as a mixture that Tear gases such as o-chlorobenzylidenemalononitrile (CS), contains a suspected human carcinogen, methylene chloride. -
(TRPM8) by Calcium-Rich Particulate Materials and Effects on Human
Molecular Pharmacology Fast Forward. Published on October 16, 2017 as DOI: 10.1124/mol.117.109959 This article has not been copyedited and formatted. The final version may differ from this version. MOL #109959 Title: Activation of Human Transient Receptor Potential Melastatin-8 (TRPM8) by Calcium- Rich Particulate Materials and Effects on Human Lung Cells Authors: John G. Lamb, Erin G. Romero, Zhenyu Lu, Seychelle K. Marcus, Hannah C. Peterson, John M. Veranth, Cassandra E. Deering-Rice, and Christopher A. Reilly Affiliations: (J.G.L., E.G.R., Z.L., S.K.M., H.C.P., J.M.V., C.E.D-R., and C.A.R.) Department of Pharmacology and Toxicology, Center for Human Toxicology, University of Utah, 30 S. 2000 E., Room 201 Skaggs Hall, Salt Lake City, UT 84112, USA. Downloaded from molpharm.aspetjournals.org at ASPET Journals on September 27, 2021 1 Molecular Pharmacology Fast Forward. Published on October 16, 2017 as DOI: 10.1124/mol.117.109959 This article has not been copyedited and formatted. The final version may differ from this version. MOL #109959 Running Title: Activation of TRPM8 by Particles *Corresponding author: Dr. Christopher A. Reilly, Ph.D. University of Utah Department of Pharmacology and Toxicology 30 South 2000 East, 201 Skaggs Hall Salt Lake City, UT 84112 Phone: (801) 581-5236; FAX: (801) 585-5111 Email: [email protected] Number of text pages: 41 Downloaded from Number of Tables: 2 Number of Figures: 7 References: 40 Number of words in Abstract: 245 Number of words in Introduction: 704 molpharm.aspetjournals.org Number -
Facts on File DICTIONARY of CHEMISTRY
The Facts On File DICTIONARY of CHEMISTRY The Facts On File DICTIONARY of CHEMISTRY Fourth Edition Edited by John Daintith The Facts On File Dictionary of Chemistry Fourth Edition Copyright © 2005, 1999 by Market House Books Ltd All rights reserved. No part of this book may be reproduced or utilized in any form or by any means, electronic or mechanical, including photocopying, recording, or by any information storage or retrieval systems, without permission in writing from the publisher. For information contact: Facts On File, Inc. 132 West 31st Street New York NY 10001 For Library of Congress Cataloging-in-Publication Data, please contact Facts On File, Inc. ISBN 0-8160-5649-8 Facts On File books are available at special discounts when purchased in bulk quantities for businesses, associations, institutions, or sales promotions. Please call our Special Sales Department in New York at (212) 967-8800 or (800) 322-8755. You can find Facts On File on the World Wide Web at http://www.factsonfile.com Compiled and typeset by Market House Books Ltd, Aylesbury, UK Printed in the United States of America MP PKG 10 9 8 7 6 5 4 3 2 1 This book is printed on acid-free paper. PREFACE This dictionary is one of a series designed for use in schools. It is intended for stu- dents of chemistry, but we hope that it will also be helpful to other science students and to anyone interested in science. Facts On File also publishes dictionaries in a variety of disciplines, including biology, physics, mathematics, forensic science, weather and climate, marine science, and space and astronomy.