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Présentée Et Soutenue Par Le 28 Décembre 2012 ANNEE: 2012 THESE N °:03/12 CSVS Faculté de Médecine et de Pharmacie de Rabat UFR Doctoral : Substance naturelles : Etude chimique et biologique Spécialité: Pharmacologie, Toxicologie et Pharmacognosie THESE DE DOCTORAT VALORISATION PHARMACOLOGIQUE D’ALOE PERRYI BAKER ET JATROPHA UNICOSTATA BALF, PLANTES ENDEMIQUES DU YEMEN: TOXICITE, POTENTIEL ANTI INFLAMMATOIRE ET ANALGESIQUE Equipe de Recherche de Pharmacodynamie ERP Présentée et soutenue par Mr. MOSA’D ALI MOSA’D AL-SOBARRY Le 28 Décembre 2012 JURY Professeur Yahia Cherrah Président Faculté de Médecine et de Pharmacie - Rabat Université Mohammed V- Souissi Professeur Katim Alaoui Directeur de Thèse Faculté de Médecine et de Pharmacie -Rabat Université Mohammed V- Souissi Professeur Amina Zellou Faculté de Médecine et de Pharmacie - Rabat Université Mohammed V - Souissi Professeur Abdelaziz Benjouad Faculté des Sciences - Rabat Rapporteurs Directeur générale de Centre National de Recherche Scientifique et Technique - Rabat Professeur Najib Gmira Faculté des Sciences - Kénitra Université Ibn Tofail -Kénitra Professeur Mohammed Akssira Faculté des Sciences et Technique - Mohammedia Examinateurs Université Hassan II - Mohammedia Professeur Naima Saidi Faculté des Sciences - Kénitra Université Ibn Tofail - Kénitra ﺑﺴﻢ اﷲ اﻟﺮﺣﻤﻦ اﻟﺮﺣﯿﻢ ﴿ﻭﻗﻞ ﺭﺏ ﺯﺩﻧﻲ ﻋﻠﻤﺎ﴾ ﺻﺪﻕ ﺍﷲ ﺍﻟﻌﻈﻴﻢ "ﺳﻮرة ﻃﮫ آﯾﺔ : 114" Je dédis cette thèse à L’âme de mes honorables grands-pères. A ma mère A mon père A ma femme et mes enfants A mes sœurs et frères A toute ma famille sans exception. Aucun mot, aucune dédicace ne sourie exprimer mon respect, ma considération et l'amour éternel que je vous porte, veuillez trouver dans ce travail toute ma gratitude. Que dieu vous protège et vous procure santé et bonheur. Ce travail a fait l’objet de: I. Publications Internationales: 1- Mosa’d Al-sobarry, Ahmed Alwashli, Yahia Cherrah and Katim Alaoui:« Anti- inflammatory Activities of Methanolic extract of Jatropha unicostata balf (Sibru) and Ethanolic extract of Aloe perryi baker (taife), as endemic plants in Yemen»; International Journal of Natural Product and Pharmaceutical Sciences ; 2011 Volume 2: Issue 2: 40-55. 2- Mosa’d Al- sobarry, Ahmed Alwashli, Yahia Cherrah and Katim Alaoui:« Analgesic Activity of Methanolic extract of Jatropha Unicostata Balf, as endemic plant in Yemen»; International Journal of Pharma and Bio Sciences ; 2011, Vol 2, Issue 4, PP. 375 -381. 3-Mosa’d Al-Sobarry, Ahmed Alwashli, Yahia Cherrah et Katim Alaoui : «« Toxicité aiguë et Action Analgésique d'extrait éthanolique des feuilles d'Aloe perryi Baker, plante endémique du Yémen» ; Phytothérapie ; Accepté , Avril 2012. 4- Ahmed Alwashli, Mosa’d Al-sobarry, Rachad Alnamer, Yahia Cherrah and Katim Alaoui :«Analgesic and Anti-inflammatory Activities of Boswellia elongate balf Methanolic Extracts, as endemic Plants in Yemen»; Journal of Biologically Active Products from Nature; 2012 Vol 2 (2) ; pp 90 – 98. 5- Ahmed Alwashli, Mosa’d Al-sobarry, Yahia Cherrah and Katim Alaoui: « Anti- inflammatory and Analgesic effects of Ethanol Extract of Dracaena Cinnabari Balf, as endemic plant in Yemen ; International Journal of Pharma and Bio Sciences ; 2012 ; vol3, issue 2. PP. 96-106. 6- A.Alwashli, M.AlSobarry, Y.Cherrah et K.Alaoui : «Toxicité aiguë et Activité Analgésique de l'extrait Méthanolique de Rumex nervosus Vahl» ; Phytothérapie, 2012 ; pp . 293-297. 7- Rachad Alnamer1, Katim Alaoui, Latifa Doudach, El Houcine Bouidida, Fatiha Chibani, Mosa’d AL-Sobarry, Abdelaziz Benjouad1, and Yahia Cherrah:«Investigation of Methanolic and Aqueous extract of Lavandula Officinalis for Toxicity and Antibacterial Activity» ; World Journal of Pharmaceutical Research; 2012 ; Vol. 1, Issue 5, PP. 1223- 1233.W 8- Rachad Alnamer1, Katim Alaoui, Latifa Doudach, El Houcine Bouidida, Fatiha Chibani, Mosa’d AL-Sobarry, Abdelaziz Benjouad1, and Yahia Cherrah: « In vitro Antibacterial activity of Rosmarinus officinalis méthanolic and aqueous extracts» ; International Journal of Pharmaceutics ; 2013; 3(1). II. Communications à des congrès Internationales: 1- Mosa’d Al- sobarry, Ahmed Alwashli, Yahia Cherrah and Katim Alaoui:« Analgesic Activity of Methanolic extract of Jatropha Unicostata Balf, as endemic plant in Yemen»; Les Journées Internationales sur Substances Naturelles et Développement Durabl. Faculté des Sciences Rabat –Agdal(Maroc) ; Les 22 – 23 Juin 2012. 2- Mosa’d Al-sobarry, Ahmed Alwashli, Yahia Cherrah and Katim Alaoui:«Anti- inflammatory Activities of Methanolic extract of Jatropha unicostata balf (Sibru) and Ethanolic extract of Aloe perryi baker (taife), as endemic plants in Yemen»; 1er International Congrès de Pharmacologie Organase par La Société Marocaine de Pharmacologie ; les 03 et 04 mai 2012. 3- Mosa’d Al-Sobarry, Ahmed Alwashli, Yahia Cherrah et Katim Alaoui : «Toxicité aiguë et Action Analgésique d'extrait éthanolique des feuilles d'Aloe perryi Baker, plante endémique du Yémen »; 1er International Congrès de Pharmacologie Organase par La Société Marocaine de Pharmacologie ; les 03 et 04 mai 2012. 4- Mosa’d Al- sobarry, Ahmed Alwashli, Yahia Cherrah and Katim Alaoui: « Analgesic Activity of Methanolic extract of Jatropha Unicostata Balf, as endemic plant in Yemen»;1er International Congrès de Pharmacologie Organase par La Société Marocaine de Pharmacologie ; les 03 et 04 mai 2012. 5- Ahmed Alwashli, Mosa’d Al-sobarry, Rachad Alnamer, Yahia Cherrah and Katim Alaoui: «Analgesic and Anti-inflammatory Activities of Boswellia elongate balf Methanolic Extracts, as endemic Plants in Yemen»; »; Les Journées Internationales sur Substances Naturelles et Développement Durabl. Faculté des Sciences Rabat – Agdal(Maroc) ; Les 22 – 23 Juin 2012. 6-A.Alwashli, M.AlSobarry, Y.Cherrah et K.Alaoui : «Toxicité aiguë et Activité Analgésique de l'extrait Méthanolique de Rumex nervosus Vahl» ; Les Journées Internationales sur Substances Naturelles et Développement Durabl. Faculté des Sciences Rabat –Agdal(Maroc) ; Les 22 – 23 Juin 2012. Je tiens à exprimer mes vifs remerciements à: Monsieur le Président de l'Université Mohammed V - Soussi, le Professeur Radouane MRABET, pour l'accueil chaleureux et pour ses aides précieuses et pour son extrême gentillesse. Madame le Doyen de la Faculté des Médecine et de Pharmacie de Rabat, le professeur Najia HAJJAJ, pour les nombreuses facilités qu'elle n'a cessé d'accorder aux étudiants étrangers inscrits en Doctorat. Madame le professeur Katim ALAOUI, responsable l'UFR: Substances naturelles et Directrice de ma thèse, pour avoir autorisé mon inscription en Doctorat : Pharmacologie et Toxicologie, pour toute l'attention et la disponibilité dont elle a fait preuve durant ces des années d'initiation à la recherche scientifique. Elle a dirigé et suivi de très près ce travail de recherche malgré ses diverses préoccupations. C'est grâce à ses suggestions, remarques et critiques que ce travail a pu être effectué. Veuillez trouver Madame dans ces mots l'expression de mes vifs remerciements et de mon profond respect. Je remercie profondément Mr. Professeur Yahia CHARRAH, Vice Doyen de la Faculté de Médecine et de Pharmacie de Rabat, Directeur de Laboratoire de Pharmacologie et Toxicologie à la Faculté de Médecine et de Pharmacie de Rabat pour son accueil au sein de son laboratoire et sans lui ce travail ne pourra avoir lieu. Votre soutien, et votre aide précieuse et votre extrême gentillesse étaient pour moi un très grand support pour la réalisation de ce travail. Veuillez trouvez dans quelques mots l'expression de mes vifs remerciements et de mon profond respect. Aussi, je vous remercie profondément pour l'honneur que vous me faites en acceptant de présider le jury de ma thèse. Toute ma reconnaissance à Mme Amina ZELLOU, Professeur à la Faculté de Médecine et de Pharmacie de Rabat, pour la bienveillante attention qu'elle a accordée à ce travail et pour la participation à ce jury. Je tiens également à remercier Monsieur le professeur Abdelaziz BENJOUAD, Professeur à la Faculté des Sciences de Rabat et Directeur du Centre National pour la Recherche Scientifique et Technique (CNRST), pour la bienveillante attention qu'il a accordé à ce travail et pour la participation à ce jury. Je remercie profondément Mr. Professeur Najib GMIRA, Professeur à la Faculté des Sciences de Kenitra, pour la bienveillante attention qu'il a accordé à ce travail. Je le remercie profondément j’avoir bien voulu accepter de siéger dans ce jury. Je remercie très respectueusement Mme. Naima SAIDI, Professeur à la Faculté des Sciences de Kenitra, pour la bienveillante attention qu'elle a accordée à ce travail et pour la participation à ce jury. Veuillez trouvez Mme l’expression de ma profonde reconnaissance. Je tiens également à remercier Monsieur le professeur Mohammed AKSSIRA, Professeur à la Faculté des Sciences et Techniques de Mohammedia, pour la bienveillante attention qu'il a accordée à ce travail et pour la participation à ce jury. Je tiens à exprimer mes sincères remerciements à tous les membres de Laboratoire de Pharmacologie et Toxicologie de notre Faculté. Aussi, je ne saurais oublier, Mr. Rashad ALNAMER, Mr. Ahmed ALWACHALI, Doctorants en Pharmacologie et Toxicologie à la Faculté de Médicine et de Pharmacie de Rabat, pour leurs aides précieuses et pour leurs extrême gentillesse. Veuillez trouvez dans quelques mots l'expression de mes vifs remerciements et de mon profond respect. Je remercie très sincèrement tous mes collègues, EL Houcine BOUIDIDA, Mostafa BOATIA, Maeen ALSUBARI, Alwan ALSUBARI, Rassam ALSUBARI, Walid ABDALRAB, Pour leurs extrêmes gentillesses. Veuillez
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