RESEARCH ARTICLE Karyotype heterogeneity in Philodendron s.l. (Araceae) revealed by chromosome mapping of rDNA loci 1 2 3,4 Emanuelle Varão VasconcelosID , Santelmo Vasconcelos , Tiago Ribeiro , Ana Maria Benko-Iseppon1, Ana Christina Brasileiro-Vidal1* 1 Departamento de GeneÂtica, Centro de Biociências, Universidade Federal de Pernambuco, CEP, Recife, Pernambuco, Brazil, 2 Instituto TecnoloÂgico Vale, CEP, BeleÂm, ParaÂ, Brazil, 3 Departamento de BotaÃnica, Centro de Biociências, Universidade Federal de Pernambuco, CEP, Recife, Pernambuco, Brazil, 4 Instituto Federal de Mato Grosso, Campus AvancËado de Diamantino, Rodovia Roberto Campos, CEP, Diamantino, a1111111111 Mato Grosso, Brazil a1111111111 a1111111111 *
[email protected] a1111111111 a1111111111 Abstract Philodendron s.l. (Araceae) has been recently focus of taxonomic and phylogenetic studies, but karyotypic data are limited to chromosome numbers and a few published genome sizes. OPEN ACCESS In this work, karyotypes of 34 species of Philodendron s.l. (29 species of Philodendron and Citation: Vasconcelos EV, Vasconcelos S, Ribeiro five of Thaumatophyllum), ranging from 2n = 28 to 36 chromosomes, were analyzed by fluo- T, Benko-Iseppon AM, Brasileiro-Vidal AC (2018) Karyotype heterogeneity in Philodendron s.l. rescence in situ hybridization (FISH) with rDNA and telomeric probes, aiming to understand (Araceae) revealed by chromosome mapping of the evolution of the karyotype diversity of the group. Philodendron presented a high number rDNA loci. PLoS ONE 13(11): e0207318. https:// variation of 35S rDNA, ranging from two to 16 sites, which were mostly in the terminal region doi.org/10.1371/journal.pone.0207318 of the short arms, with nine species presenting heteromorphisms. In the case of Thaumato- Editor: Arthur J.