The Genus Amanita 3Huv ,1/80%,1,=21( Nepal
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THE GENUS AMANITA 3HUV ,1/80%,1,=21( NEPAL Hari Prasad Aryal * and Usha Budhathoki ** 3DNOLKDZD&DPSXV,QVWLWXWHRI$JULFXOWXUHDQG$QLPDO6FLHQFH%KDLUDKDZD &HQWUDO'HSDUWPHQWRI%RWDQ\7ULEKXYDQ8QLYHUVLW\.LUWLSXU.DWKPDQGX1HSDO Abstract: 'XULQJWKHVXUYH\RIZLOGHGLEOHPXVKURRPVLQWURSLFDOWRWHPSHUDWHEHOWRI1HSDOGXULQJUDLQ\VHDVRQVLQ DQGPDQ\PDFURIXQJDOVSHFLHVZHUHFROOHFWHGDQGLGHQWL¿HG7KHSDSHUKLJKOLJKWVRQWKH¿YHVSHFLHVRI genus Amanita 3HUVLQFOXGLQJQHZVSHFLHV Amanita volvata 3HFN /OR\G ZLWKWKHLULGHQWL¿FDWLRQDQGGRFXPHQWDWLRQ The new species was LGHQWL¿HGDQGWKLVLVEHLQJUHSRUWHGDQGUHGHVFULEHGIRUWKH¿UVWWLPHIURP1HSDO Amanita characterized by stipe with volva or rudiments of volva, annulus present, in some may be absent, clamp connection SUHVHQWRUDEVHQWVSRUHVDP\ORLGRULQDP\ORLG7KHVWXG\DUHDRFFXSLHGVTNPDPRQJWKHVT.PODQG DQGOLHVZLWKLQDQDOWLWXGHEHWZHHQDQGPDVOLQWURSLFDOGHFLGXRXVULYHULQHIRUHVWWRORZHUWHPSHUDWHPL[HG EURDGOHDYHGIRUHVW7KHGULHGVSHFLPHQVDUHKRXVHGLQWKH7ULEKXYDQ8QLYHUVLW\&HQWUDO+HUEDULXP 78&+ .LUWLSXU .DWKPDQGX1HSDO7KHDUHDHPEUDFHVPDQ\P\FRSKDJRXVHWKQLFFRPPXQLWLHV7KHP\FRHOHPHQWVSUHYDLOLQJLQWKLV DUHDQHHGVXVWDLQDEOHFRQVHUYDWLRQDQGXWLOL]DWLRQ Keywords: Tasonomy; %DVLGLRP\FHWHV Amanita volvata; 0DFURIXQJL INTRODUCTION 7KH LQYHVWLJDWLRQ DQG VWXG\ RQ PXVKURRPV RI 1HSDO started since 19 th FHQWXU\ /OR\G %HUNHOH\ 7KH JHQXV Amanita FRQWDLQV RYHU QDPHG VSHFLHV DQG YDULHWLHV $GKLNDUL et al Amanita IURP1HSDOKDVEHHQUHSRUWHGVLQFH th century (Singh, DE VLQFHWKHQVHYHUDOSDSHUVKDYHEHHQSXEOLVKHG 42 species and 2 subspecies of Amanita are reported IURP1HSDO $GKLNDUL et al VLQFHWKHQVHYHUDO ERWDQLFDOLQYHVWLJDWLRQVKDYHEHHQGRQH$PRQJWKHVH very few reveal the studies and investigation on wild PXVKURRPVIURPZHVWHUQ1HSDO7KLVDUHDKDVQRWEHHQ SUHYLRXVO\ LQYHVWLJDWHG VR IDU This is a preliminary report on KLJKOLJKWRQSUHVHQWO\¿YHWD[DRIWKHJHQXV Amanita 3HUV ZLWK WKH DGGLWLRQ RI QHZ VSHFLHV IURP /XPELQL]RQHDUHJLRQRIWURSLFDOWRWHPSHUDWHPRQVRRQ FOLPDWH DQG ZHVWVRXWK UHJLRQ RI 1HSDO Amanita volvata 3HFN /OR\G ZDV LGHQWL¿HG DQG WKLV LV EHLQJ UHSRUWHGDQGUHGHVFULEHGIRUWKH¿UVWWLPHIURP1HSDO KDYH QRW UHSRUWHG SUHYLRXVO\ EHHQ UHFRUGHG7KH QHZ Figure 1: Sample Collection sites ¿QGLQJVKDYHSXEOLVKHGKHUH 5VXQJD*XOPL P RI PLGZHVW UHJLRQ RI 1HSDO o o STUDY AREA ,W OLHV EHWZHHQ 1 ODWLWXGH DQG o ( ORQJLWXGH 7KH YHJHWDWLRQ LQ 7KHVWXG\DUHD ¿J OLHVLQ/XPELQL=RQH3DUWLDOSDUWV forest is dominated by members of the Combretaceae, RI 3DNOLKDZD5XSDQGHKL P 7LNNDU.DSLOZDVWX 'LSWHURFDUSDFHDH)DJDFHDHDQG/HJXPLQRVDH7KHDUHD P 5DPQDJDU1DZDOSDUDVL P 7KDGD RFFXSLHVKDODQGEHWZHHQDQGPDVO7KH $UJKDNKDQFKL P 6LPDOGDQGD3DOSD P DQG Author for Correspondence : +DUL3UDVDG$U\DO3DNOLKDZD&DPSXV,QVWLWXWHRI$JULFXOWXUHDQG$QLPDO6FLHQFH%KDLUDKDZD(PDLOKDKDULSUDVDGDU\DO#JPDLOFRP 113 Scientic World, Vol. 11, No. 11, July 2013 DYHUDJHDQQXDOUDLQIDOOLVPP RESULTS: Phytogeographically the area lies in tropical and 1. Amanita volvata 3HFN /OR\G temperate zones embracing interesting different types Amanita volvata I DYHOODQHRVTXDPRVD 6 ,PDL (- RI IRUHVWV DQG VRLO FRPSRVLWLRQ 7KH WURSLFDO ULYHULQH *LOEHUW LQ %UHVDGROD Iconogr. Mycol. 6XSSO belt composed of Acacia cateachu, Adina cordifolia, Anogeissus latifolius, Bombax ceiba, Dalbergia Local name %HVDUHFK\DX latifolia, Dalbergia sissoo, Eugeina jambolana, Taxonomic position %DVLGLRP\FHWHV $JDULFDOHV Mallotus philippensis, Phoenix sylvestris, Shorea Amanitaceae )LJXUHDQG robusta, Schleihera oleosa, Syzygium cumini, Tectona grandis, Terminalia alata, T. tomentosa and T. belerica HWFZLWKDQLQFUHDVLQJDOWLWXGHRI Castanopsis indica, Ehretia acuminata, Leucosceptrum canum, Lithocarpus elegans, mallotus nepalensis, Quercus lamellosa, Quercus semecarpifolia, Rhododendron arboreum and Schima wallichii. The northern belt of this area has loamy sand, while the southern belt consists of sandy ORDPWRORDP\VRLO MATERIALS AND METHODS Surveyed was done from 15 th to 31 th 0D\DQGVSHFLPHQV were collected from 1 st -XQH WR st 2FW LQ DQG 0XVKURRP VDPSOHV ZHUH SKRWRJUDSKHG LQ WKHLU natural habitat and their morphological characters were QRWHG6SRUHSULQWVZHUHWDNHQ7KHVDPSOHVZHUHZHOO GULHGDQGSDFNHGLQZD[SDSHUEDJVDQGZUDSSLQJZLWK Figure 1.1: Amanita volvata aluminums foil to prevent from the external infection as well as prevent from the intermixing of the spores, with SURSHU WDJ QXPEHUV 7KH KDELWDW LQFOXGLQJ HFRORJLFDO SDUDPHWHUV YL] DOWLWXGH E\ DOWLPHWHU YHJHWDWLRQ composition, soil type, soil pH (by digital pH meter), soil moisture (by nail pH meter), humidity, temperatures and WLPH E\PHDQVRIWKHUPR+\JURPHWHU ZHUHUHFRUGHG The paper bags were brought to central Department of ERWDQ\ 7ULEKXYDQ 8QLYHUVLW\ IRU IXUWKHU PLFURVFRSLF H[DPLQDWLRQ Some of the collected specimens were also preserved LQDOLTXLGSUHVHUYDWLYH POUHFWL¿HGDOFRKRO IRUPDOLQGLVWLOOHGZDWHU +DZNVZRUWK et. al., The specimens were studied based on the macroscopic, PLFURVFRSLF DQG KDELWDW FKDUDFWHULVWLFV ;DQWKRFKRULF reaction (Tulloss, 1994) was determined by placing a Figure 1.2: Spores print VPDOOSLHFHRIPDWHULDOLQ.2+VROXWLRQIROORZHG WRWKH1+ 32+ ,DQG)H62 6ROXWLRQ &U\VWDO DQGLWV colour compared with the commercial colour chart leaf- OHW)RUPLFURVFRSLFVWXG\WKHVSHFLPHQVZHUHVHFWLRQHG E\ XVLQJ D VKDUS UD]RU EODGH 7KH F\DQRSKLOLW\ ZDV REVHUYHGLQFRWWRQEOXHPRXQWHGLQODFWRSKHQROUHDJHQW 7KHSKRWRJUDSKVZHUHWDNHQZLWKD621<'6&6 Camera, macroscopic and microscopic characters were VWXGLHGXQGHUWKHFRPSRXQGPLFURVFRSH2O\PSXV1R 7RN\R -DSDQ DQGODWLWXGHDQGORQJLWXGHZHUH WDNHQE\PHDQVRI*36FRPSDVV 7KHYRXFKHUVSHFLPHQVZHUHLGHQWL¿HGZLWKWKHKHOSRI UHOHYDQWWD[RQRPLFOLWHUDWXUHYL] 6LQJHU.XPDU et al., DQG:DWNLQVRQ et al PRQRJUDSK *HUULW DQG :HEVLWH -VWRU RUJ ,QGH[ IXQJRUXP WURSLFRVRUJ 0\FREDQNRUJ ELRGLYHUVLW\ OLEUDU\RUJ 7KH YRXFKHU VSHFLPHQV DUH )LJXUH6SRUHV GLY P GHSRVLWHGLQ78&+1HSDO Scientic World, Vol. 11, No. 11, July 2013 114 Identifying characters 7KH VSHFLHV LV TXLWH UDUH Absence of annulus and the presence of the large VRPHZKDW FXSVKDSHG SHUVLVWHQW YROYD 7KH SLOHXV LV not smooth as is usually the case in the species with a persistent membranous volva, but it is more or less VFDO\ ZLWK PLQXWH WXIWV RI ¿EULOV RU WRPHQWRVH KDLUV 6RPHWLPHVWKHPDUJLQLVQRWYHU\GLVWLQFWO\VWULDWH7KH FRORXU YDULHV IURP ZKLWH WR EURZQLVK 7KH ODPHOODH which are white in the fresh, in dried specimens assume a dull yellowish-brown colour, except on the edge which UHPDLQVZKLWHDQGLVPRUHRUOHVVÀRFFRVH -XVWL¿FDWLRQ : Another species of A. volvaceus %XOOKDV VLPLODUYROYDEXWLWVVSRUHDQGODPHOODHDUHSLQNLVKRU ÀHVKFRORXUHGDQGLWEHORQJVWKHUHIRUHWRWKHVXEJHQXV Volvaria +HQFHWKLVQHZVSHFLHV A. volvata distinctly Figure 2.1: Amanita caesarea, differed from the A volvaceus Taxonomic description: Pileus: FP FRQYH[ WR ÀDW PDUJLQ ZLWK UDGLDO VWULDWLRQV KDLU\ RU ÀRFFRVHVFDO\ ZKLWH WR FUHDP pale yellow; surface often with numerous patches RI WKLQ YHLO UHPQDQWV Gills : free, broad, crowded; ZKLWH Stipe [FPZKLWHVXUIDFHÀRFFRVH scaly to powdery, also discoloring; slightly tapering XSZDUG EDVH HPHUJLQJ IURP D WKLFN PHPEUDQRXV ÀHVK\ VDFFDWH YROYD SHUVLVWHQW QR ULQJ LV OHIW RQ WKH VWLSHEXOERXVEDVH Flesh: white, discolour when cut, EUXLVHGRUGDPDJHG Odor QRWGLVWLQFWLYH Taste : not Figure 2.2: Spores print GLVWLQFWLYH Spores : elliptic, smooth ornamentation, FHOOZDOOWKLFNFRORXUK\DOLQHDP\ORLGGHSRVLWZKLWH FUHDPRU\HOORZLVKPHDVXUHPHQW[PLQVL]H Spore print ZKLWH Habitat LQRSHQ¿HOGRIWHQVDQG\VRLOVLQPL[HGZRRGV DQG URDGVLGHV 5DUH QRW FRPPRQ )RXQG LQ HDVWHUQ 1RUWK $PHULFD Ecology 0\FRUUKL]DO Season: -XO\ 2FWREHU Edibility 3RLVRQRXV1RWHGLEOH Chemical reaction 7XOORVV5( : :LWK1+ 3 :negative on all the SDUWV :LWK.2+ \HOORZLVKJUH\RQFDSVXUIDFH :LWK )H6R 4 solution: 6WLSH RXWHU QHJDWLYH LQQHU %HDFRQ&DSQHJDWLYH*LOOV5DZ6LON :LWK )H6R 4 crystal: Stipe outer: negative, inner: %HDFRQ&DSQHJDWLYH*LOOV6DQGDOZRRG Specimen examined: 1HSDO 5XSDQGHKL 'XGUDFKK\D :1PORQJ ¶1ODW ¶( &ROO1RRQKXPXVULFKOLWHUVRLOLQ6DOIRUHVW $U\DO+31HZWR1HSDO&RPPRQ Distribution 1RUWK$PHULFDDQG1HSDO )LJXUH6SRUHV GLY P 2. Amanita caesarea 6FRS 3HUV 6\QPHWKIXQJ *RWWLQJHQ 2 PDUJLQ VWULDWH Gills: IUHH FURZGHG \HOORZ Stipe: &DHVDU¶VPXVKURRP (QJ cylindrical, 8-15 x 2-3 cm, narrowing at the top, hollow Local name: Suntale chyau, Salle chyau, Phul chyau, when mature, with yellow falling annulus, slightly 'KDUVK\DPR 7DPDQJ swollen at the base, with large white membranous Taxonomic position %DVLGLRP\FHWHV $JDULFDOHV YROYD Flesh: whitish, \HOORZLVKEHQHDWKFXWLFOH Odor : $PDQLWDFHDH )LJXUHDQG ZLWKRXWHYLGHQW Taste: JRRG Spores: elliptical, oval to Taxonomic description: VSKHULFDORUQDPHQWDWLRQVPRRWKFHOOZDOOWKLFNFRORXU Pileus: FP KHPLVSKHULFDO WR ÀDW RUDQJH UHG K\DOLQHZKLWHPHDVXUHPHQW[PLQVL]H washing out to yellow, cuticle separable sometimes with Spore print: ZKLWH evident membranous remains of white veil, 115 Scientic World, Vol. 11, No. 11, July 2013 Habitat: 6RLO RQ 4XHUFXV5KRGRGHQGURQ IRUHVW Ecology P\FRUUKL]DO Season -XQH$XJXVW Edibility: (GLEOHH[FHOOHQW8VHGDVYHJHWDEOH Chemical reaction 7XOORVV5( : :LWK1+ 3 UHGGLVKRQFDSVXUIDFH :LWK.2+ \HOORZLVKJUH\RQFDSVXUIDFH :LWK)H6R 4 solution : 6WLSH RXWHU QHJDWLYH LQQHU 3DOH &UHDP &DS QHJDWLYH *LOOV ,YRU\ :LWK )H6R 4 crystal: Stipe: outer: negative, inner: $QWLTXH :KLWH &DS QHJDWLYH *LOOV 0DFUDPH Specimen examined: 1HSDO 5XSDQGHKL 'XGUDFKK\D Figure 3.1: A manita chepangiana, :1PORQJ ¶1ODW ¶( &ROO1RRQKXPXVULFKOLWHUVRLOLQ6DOIRUHVW $U\DO +3 1HZ WR DUHD RI FROOHFWLRQ (GLEOH5V.J&RPPRQ Previously reported: 0DQLFKXU $GKLNDUL .DWKPDQGX PDUNHW $GKLNDULD JURZLQJRQVRLO,QPRLVWVKDG\SODFH in Pinus roxburghii IRUHVW %DMUD\RJLQL P QR ) $GKLNDULE .DWKPDQGXPDUNHW $GKLNDUL DQG$GKLNDUL JURZLQJRQVRLOLQPRLVWVKDG\ place, in Pinus roxburghii