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CCDC22 Shrna (H) Lentiviral Particles: Sc-91292-V

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CCDC22 Shrna (H) Lentiviral Particles: Sc-91292-V SANTA CRUZ BIOTECHNOLOGY, INC. CCDC22 shRNA (h) Lentiviral Particles: sc-91292-V BACKGROUND APPLICATIONS CCDC22 (coiled-coil domain-containing protein 22), also known as JM1 or CCDC22 shRNA (h) Lentiviral Particles is recommended for the inhibition of CXorf37, is a 627 amino acid cytoplasmic and nuclear protein. The gene that CCDC22 expression in human cells. encodes CCDC22 maps to human chromosome X, which consists of about 153 million base pairs and nearly 1,000 genes. The combination of an X and Y SUPPORT REAGENTS chromosome lead to normal male development while two copies of X lead to Control shRNA Lentiviral Particles: sc-108080. Available as 200 µl frozen normal female development. There are a number of conditions related to an viral stock containing 1.0 x 106 infectious units of virus (IFU); contains an unsual number and combination of sex chromosomes being inherited. More shRNA construct encoding a scrambled sequence that will not lead to the than one copy of the X chromosome with a Y chromosome causes Klinefelter’s specific degradation of any known cellular mRNA. syndrome or single copy of X, alone, leads to Turner’s syndrome. More than two copies of the X chromosome, in the absence of a Y chromosome, is known RT-PCR REAGENTS as triple X syndrome. Color blindness, hemophilia and Duchenne muscular dys- trophy are well known X chromosome-linked conditions which affect males Semi-quantitative RT-PCR may be performed to monitor CCDC22 gene more frequently, as males carry a single X chromosome. expression knockdown using RT-PCR Primer: CCDC22 (h)-PR: sc-91292-PR (20 µl). Annealing temperature for the primers should be 55-60° C and the REFERENCES extension temperature should be 68-72° C. 1. Givens, J.R., et al. 1975. Features of Turner’s syndrome in women with BIOSAFETY polycystic ovaries. Obstet. Gynecol. 45: 619-624. Lentiviral particles can be employed in standard Biosafety Level 2 tissue 2. Bernardino-Sgherri, J., et al. 2002. Overall DNA methylation and chro- culture facilities (and should be treated with the same level of caution as matin structure of normal and abnormal X chromosomes. Cytogenet. with any other potentially infectious reagent). Lentiviral particles are repli- Genome Res. 99: 85-91. cation-incompetent and are designed to self-inactivate after transduction 3. Tomsig, J.L., et al. 2003. Identification of targets for calcium signaling and integration of shRNA constructs into genomic DNA of target cells. through the copine family of proteins. Characterization of a coiled-coil copine-binding motif. J. Biol. Chem. 278: 10048-10054. RESEARCH USE 4. Deeb, S.S. 2005. The molecular basis of variation in human color vision. The purchase of this product conveys to the buyer the nontransferable right Clin. Genet. 67: 369-377. to use the purchased amount of the product and all replicates and derivatives for research purposes conducted by the buyer in his laboratory only (whether 5. Bojesen, A., et al. 2006. The metabolic syndrome is frequent in Klinefelter’s the buyer is an academic or for-profit entity). The buyer cannot sell or other- syndrome and is associated with abdominal obesity and hypogonadism. wise transfer (a) this product (b) its components or (c) materials made using Diabetes Care 29: 1591-1598. this product or its components to a third party, or otherwise use this product 6. Hayashi, T., et al. 2006. Novel form of a single X-linked visual pigment gene or its components or materials made using this product or its components in a unique dichromatic color-vision defect. Vis. Neurosci. 23: 411-417. for Commercial Purposes. CHROMOSOMAL LOCATION PROTOCOLS Genetic locus: CCDC22 (human) mapping to Xp11.23. See our web site at www.scbt.com for detailed protocols and support products. PRODUCT CCDC22 shRNA (h) Lentiviral Particles is a pool of concentrated, transduction- ready viral particles containing 3 target-specific constructs that encode 19- 25 nt (plus hairpin) shRNA designed to knock down gene expression. Each vial contains 200 µl frozen stock containing 1.0 x 106 infectious units of virus (IFU) in Dulbecco’s Modified Eagle’s Medium with 25 mM HEPES pH 7.3. Suitable for 10-20 transductions. Also see CCDC22 siRNA (h): sc-91292 and CCDC22 shRNA Plasmid (h): sc-91292-SH as alternate gene silencing products. STORAGE Store lentiviral particles at -80º C. Stable for at least one year from the date of shipment. Once thawed, particles can be stored at 4º C for up to one week. Avoid repeated freeze thaw cycles. Santa Cruz Biotechnology, Inc. 1.800.457.3801 831.457.3800 fax 831.457.3801 Europe +00800 4573 8000 49 6221 4503 0 www.scbt.com.
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