Efficient Professional Protein and Antibody Platforms CCDC47 Antibody Basic information: Catalog No.: UMA60617 Source: Mouse Size: 50ul/100ul Clonality: Monoclonal Concentration: 1mg/ml Isotype: Mouse IgG1 Purification: -- Useful Information: WB:1:500-1:1000 ICC:1:50-1:200 Applications:

IHC:1:50-1:200 Reactivity: Human, Mouse Specificity: This antibody recognizes CCDC47 protein. Immunogen: Recombinant protein Coiled-coil domain 47 (CCDC47) is a gene located on human 17, specifically locus 17q23.3 which encodes for the protein CCDC47. The gene has several aliases including GK001 and MSTP041. The protein itself contains coiled-coil domains, the SEEEED superfamily, a domain of unknown function (DUF1682) and a transmembrane domain. The function of the protein is unknown, but it has been proposed that CCDC47 is involved in Description: calcium ion homeostasis and the endoplasmic reticulum overload response. In addition to the high levels of seen in CCDC47, three sul- fination sites are predicted and conserved in mammals, reptiles and birds but not in fish, amphibians or invertebrates. Five potential sumoylation sites are also seen and conserved back to the bony fish. There is no glycosylation of the protein as it is not predicted to extend into the extracellular portion of the cell. Uniprot: Q96A33 (human) BiowMW: 56 kDa Buffer: 1*TBS (pH7.4), 1%BSA, 40%Glycerol. Preservative: 0.05% Sodium Azide. Storage: Store at 4°C short term and -20°C long term. Avoid freeze-thaw cycles. Note: For research use only, not for use in diagnostic procedure. Data:

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected] Efficient Professional Protein and Antibody Platforms Western blot analysis of CCDC47 on recombinant protein using anti-CCDC47 antibody at 1/1,000 dilution.

ICC staining CCDC47 in F9 cells (red). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

ICC staining CCDC47 in MCF-7 cells (red). The nu- clear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunohistochemical analysis of paraf- fin-embedded human lung tissue using an- ti-CCDC47 antibody. Counter stained with hema- toxylin.

Gene Universal Technology Co. Ltd www.universalbiol.com Tel: 0550-3121009 E-mail: [email protected]