DOCSLIB.ORG

(12) United States Patent (10) Patent No.: US 9,371.369 B2 Long Half-Life

Total Page：16

File Type：pdf, Size：1020Kb

Download full-text PDF Read full-text
(12) United States Patent (10) Patent No.: US 9,371.369 B2 Long Half-Life US009371369B2 (12) United States Patent (10) Patent No.: US 9,371.369 B2 Schellenberger et al. 45) Date of Patent: *Jun. 21,9 2016 (54) EXTENDED RECOMBINANT POLYPEPTIDES (52) U.S. Cl. AND COMPOSITIONS COMPRISING SAME CPC ............. C07K 14/545 (2013.01); C07K 14/001 (71) Applicant: Amunix Operating Inc., Mountain (2013.01). C07K 14/47 (2013.01); View, CA (US) (Continued) 72 I t : Volk Schellenb Palo Alto, CA (58) Field of Classification Search (72) Inventors: (US);Volker Joshua schellenberger, Silverman, Palo Sunnyvale, Alto, CA CPC ... C07K 14/001; C07K 14/47; C07K 14/545; - 0 C07K 14/605; C07K 14/61; C07K 14/745: (US); Chia-Wei Wang, Milpitas, CA C07K 2319/31: C07K 2319/35 (US); Benjamin Spink, San Carlos, CA See application file for complete search history. (US); Willem P. Stemmer, Los Gatos, CA (US); Nathan Geething, Santa (56) References Cited Clara, CA (US); Wayne To, Fremont, CA (US); Jeffrey L. Cleland, San U.S. PATENT DOCUMENTS Carlos, CA (US) 3,992,518. A 1 1/1976 Chien et al. (73) Assignee: Amunix Operating Inc., Mountain 4,088,864 A 5, 1978 Theeuwes et al. View, CA (US) (Continued) (*) Notice: Subject to any disclaimer, the term of this FOREIGN PATENT DOCUMENTS patent is extended or adjusted under 35 U.S.C. 154(b) by 78 days. CN 1761684. A 4, 2006 This patent is Subject to a terminal dis- CN 1933855. A 3, 2007 claimer. (Continued) (21) Appl. No.: 14/168,973 OTHER PUBLICATIONS (22) Filed: Jan. 30, 2014 Kangueane; et al., “T-Epitope Designer: A HLA-peptide binding (65) Prior Publication Data prediction server”. May 15, 2005, 1(1), 21-4. (Continued) US 2014/O3O1974 A1 Oct. 9, 2014 Primary Examiner — James H Alstrum Acevedo Related U.S. Application Data Assistant Examiner — Randall L. Beane (63) Continuation of application No. 12/699,761, filed on R ttorney, Agent, or Firm — Wilson Sonsini Goodrich & Feb. 3, 2010, now Pat. No. 8,673,860. (57) ABSTRACT (60) Provisional application No. 61/149,669, filed on Feb. The present invention relates to compositions comprising 3, 2009, provisional application No. 61/268,193, filed biologically active proteins linked to extended recombinant (Continued) polypeptide (XTEN), isolated nucleic acids encoding the compositions and vectors and host cells containing the same, (51) Int. Cl. and methods of using Such compositions in treatment of glu A6 IK38/6 (2006.01) cose-related diseases, metabolic diseases, coagulation disor A6 IK38/8 (2006.01) ders, and growth hormone-related disorders and conditions. (Continued) 37 Claims, 48 Drawing Sheets Long half-life LOW clotting activity XTEN release Short half-life High clotting activity US 9,371.369 B2 Page 2 Related U.S. Application Data 5,599,907 2, 1997 Anderson et al. 5,610,278 3, 1997 Nordfang et al. on Jun. 8, 2009, provisional application No. 61/185. 5,660,848 8, 1997 Moo-Young 112, filed on Jun. 8, 2009, provisional application No. 5,756,115 5, 1998 Moo-Young et al. 5,789,379 8, 1998 Drucker et al. 61/236,493, filed on Aug. 24, 2009, provisional appli 5,833,911 11, 1998 Llort et al. cation No. 61/236,836, filed on Aug. 25, 2009, provi 5,874, 104 2, 1999 Adler-Moore et al. sional application No. 61/243,707, filed on Sep. 18, 5,916,588 6, 1999 Popescu et al. 2009, provisional application No. 61/245.490, filed on 5,919,766 7, 1999 Osterberg et al. 5,942,252 8, 1999 Tice et al. Sep. 24, 2009, provisional application No. 61/280,955, 5,965,156 10, 1999 Profitt et al. filed on Nov. 10, 2009, provisional application No. 5,972,885 10, 1999 Spira et al. 61/280,956, filed on Nov. 10, 2009, provisional appli 5,981,719 11, 1999 WoisZwillo et al. 6,024,983 2, 2000 Tice et al. cation No. 61/281,109, filed on Nov. 12, 2009. 6,043,094 3, 2000 Martin et al. 6,048,720 4, 2000 Dalborg et al. (51) Int. C. 6,056,973 5/2000 Allen et al. C07K I4/6 (2006.01) 6,060,447 5/2000 Chapman et al. C07K I4/46 (2006.01) 6,090,925 T/2000 WoisZwillo et al. C07K 4/545 (2006.01) 6,110,498 8, 2000 Rudnic et al. 6,126,966 10, 2000 Abra et al. C07K I4/00 (2006.01) 6,183,770 2, 2001 Muchin et al. C07K I4/605 (2006.01) 6,251,632 6, 2001 Lillicrap et al. C07K I4/745 (2006.01) 6,254,573 T/2001 Haim et al. C07K I4/47 (2006.01) 6,268,053 T/2001 WoisZwillo et al. U.S. C. 6,284.276 9, 2001 Rudnic et al. (52) 6,294, 170 9, 2001 Boone et al. CPC .............. C07K 14/605 (2013.01); C07K 14/61 6,294,191 9, 2001 Meers et al. (2013.01); C07K 14/745 (2013.01); C07K 6,294,201 9, 2001 Kettelhoit et al. 2319/31 (2013.01); C07K 2319/35 (2013.01) 6,303,148 10, 2001 Hennink et al. 6,309,370 10, 2001 Haim et al. References Cited 6,316,024 11, 2001 Allen et al. (56) 6,316,226 11, 2001 Van Ooyen et al. U.S. PATENT DOCUMENTS 6,329, 186 12, 2001 Nielsen et al. 6,346,513 2, 2002 Van Ooyen et al. 6,352,716 3, 2002 Janoff et al. 4.200,984 A 5, 1980 Fink 6,352,721 3, 2002 Faour 4,284.444 A 8, 1981 Bernstein et al. 6,361,796 3, 2002 Rudnic et al. 4,398,908 A 8/1983 Siposs 6,395,302 5/2002 Hennink et al. 4,435,173 A 3/1984 Siposs et al. 6,406,632 6, 2002 Safir et al. 4,542,025 A 9, 1985 Tice et al. 6,406,713 6, 2002 Janoff et al. 4,599,311 A 7, 1986 Kawasaki 6,458,387 10, 2002 Scott et al. 4,684,479 A 8/1987 D'Arrigo 6,458,563 10, 2002 Lollar 4,713,339 A 12/1987 Levinson et al. 6,514,532 2, 2003 Rudnic et al. 4,757,006 A 7/1988 Toole, Jr. et al. 6,517,859 2, 2003 Tice et al. 4,845,075 A 7/1989 Murray et al. 6,534,090 3, 2003 Puthli et al. 4,861,800 A 8/1989 Buyske 6,572,585 6, 2003 Choi 4,870,008 A 9, 1989 Brake 6,669,961 12, 2003 Kim et al. 4,882.279 A 1 1/1989 Cregg 6,713,086 3, 2004 Qiu et al. 4,897.268 A 1/1990 Tice et al. 6,715.485 4, 2004 Djupesland 4,931,373 A 6, 1990 Kawasaki et al. 6,733,753 5, 2004 Boone et al. 4,933,185 A 6/1990 Wheatley et al. 6,743,211 6, 2004 Prausnitz et al. 4,965,199 A 10/1990 Capon et al. 6,759,057 T/2004 Weiner et al. 4,976,696 A 12/1990 Sanderson et al. 6,814,979 11, 2004 Rudnic et al. 4,988,337 A 1, 1991 to 6,818.439 11, 2004 Jolly et al. 5,004,804 A 4, 1991 Kuo et al. 6,838,093 1/2005 Flanner et al. 5,017,378 A 5, 1991 Turner et al. 6,890,918 5/2005 Burnside et al. 5,037,743 A 8, 1991 Welch et al. 6,905,688 6, 2005 Rosen et al. 5,089,474 A 2f1992 Castro et al. 6,919,311 7/2005 Lenting et al. 5, 112,950 A 5, 1992 Meulien et al. 6,945,952 9, 2005 Kwon 5,171,844 A 12/1992 van Ooyen et al. 7,045,318 5/2006 Ballance 5,176,502 A 1/1993 Sanderson et al. 7,276.475 10, 2007 Defrees et al. 5, 186,938 A 2/1993 Sablotsky et al. 7,294,513 11/2007 Wyatt 5, 198,349 A 3, 1993 Kaufman 7,329,640 2, 2008 Vlasuk 5,215,680 A 6/1993 D'Arrigo 7,413,537 8, 2008 Ladner et al. 5,223,409 A 6, 1993 Ladner et al. 7,442,778 10, 2008 Gegg et al. 5,250,421 A 10, 1993 Kaufman et al. 7,452,967 11/2008 Bertin 5,270,176 A 12/1993 Dorschug et al. 7,511,024 3, 2009 Pederson et al. 5,298,022 A 3, 1994 Bernardi 4/2009 Lee et al. 5,318.540 A 6/1994 Athayde et al. 7,514,257 5,364,934 A 1 1/1994 Drayna et al. 7,528,242 5/2009 Anderson et al. 5,407,609 A 4/1995 Tice et al. 7,560, 107 T/2009 Lollar 5,424,199 A 6, 1995 Goeddeletal. 7,709,605 5, 2010 Knopfet al. 5.492.534 A 2/1996 Athayde et al. 7,846.445 12/2010 Schellenberger et al. 5,534,617 A 7, 1996 Cunningham et al. 8,129,348 3, 2012 Besman et al. 5,543,502 A 8/1996 Nordfang et al. 8, 178.495 5, 2012 Chilkoti 5,554,730 A 9, 1996 WoiSZwillo et al. 8.492,530 T/2013 Schellenberger et al. 5,573,776 A 11/1996 Harrison et al. 8,557,961 10, 2013 Silverman et al. 5,576,291 A 11/1996 Curtis et al. 8,673,860 3, 2014 Schellenberger et al. 5,578,709 A 11/1996 WoiSZwillo 8,680,050 3, 2014 Schellenberger et al.
Load more

Recommended publications
  • APPENDIX H – Bibliography of ECOTOX Open Literature
    APPENDIX H – Bibliography of ECOTOX Open Literature Refresh from 2012 Accepted by ECOTOX and OPP Reference List 1. Abbott, J. D.; Bruton, B. D., and Patterson, C. L. Fungicidal Inhibition of Pollen Germination and Germ-Tube Elongation in Muskmelon. REPSOIL,ENV; 1991; 26, (5): 529-530. Notes: EcoReference No.: 63745 Chemical of Concern: BMY,CTN,CuOH,MZB 2. Abney, M. R.; Ruberson, J. R.; Herzog, G. A.; Kring, T. J.; Steinkraus, D. C., and Roberts, P. M. Rise and Fall of Cotton Aphid (Hemiptera: Aphididae) Populations in Southeastern Cotton Production Systems. GRO,POP. Department of Entomology, North Carolina State University, Raleigh, NC, USA. [email protected]//: SOIL,ENV; 2008; 101, (1): 23-35. Notes: EcoReference No.: 156197 Chemical of Concern: AZX,CTN,IMC 3. Al-Dosari, S. A.; Cranshaw, W. S., and Schweissing, F. C. Effects on Control of Onion Thrips from Co- Application of Onion Pesticides. POPENV; 1996; 21, (1): 49-54. Notes: EcoReference No.: 90255 Chemical of Concern: CTN,CYP,CuOH,LCYT,MLX,MMM,Maneb 4. Arts, G. H. P.; Belgers, J. D. M.; Hoekzema, C. H., and Thissen, J. T. N. M. Sensitivity of Submersed Freshwater Macrophytes and Endpoints in Laboratory Toxicity Tests. GROAQUA; 2008; 153, (1): 199-206. Notes: EcoReference No.: 108008 Chemical of Concern: ASM,CTN,FNZ,PCP 5. Aziz, T.; Habte, M., and Yuen, J. E. Inhibition of Mycorrhizal Symbiosis in Leucaena leucocephala by Chlorothalonil. BCM,GRO,POPSOIL,ENV; 1991; 131, (1): 47-52. Notes: EcoReference No.: 92163 Chemical of Concern: CTN 6. Babu, T. H. Effectiveness of Certain Chemicals and Fungicides on the Feeding Behaviour of House Sparrows.
    [Show full text]
  • English-Persian
    English-Persian English-Persian A abrachia (= abrachiatism) ﺑﯽ دﺳﺘﯽ ﻣﺎدرزاد abrachiatism ﺑﯽ ﺳﺮو دﺳﺘﯽ ﻣﺎدر زاد abrachiocephalia ﺁ. (A (= adenine ﺑﯽ ﺳﺮ و دﺳﺖ ﻣﺎدر زاد abrachiocephalus ﺁ. (A (= adenovirus ﺑﯽ دﺳﺖ ﻣﺎدر زاد abrachius زﻧﺠﻴﺮﻩ ﺁ. A chain اﺑﺮﯾﻦ abrin دﯼ.ان.اﯼ ﻧﻮع ﺁ. A form DNA اﺑﺴﻴﺰﯾﮏ اﺳﻴﺪ abscisic acid ﺁ.ﯾﮏ، اﯼ.وان. A I (= first meiotic ﭘﻴﮑﺮ anaphase) absolute configuration ﺑﻨﺪﯼ/ﮐﻨﻔﻴﮕﻮراﺳﻴﻮن/ﺁراﯾ ﺁ.دو، اﯼ.ﺗﻮ. A II (= second meiotic ش ﻣﻄﻠﻖ (anaphase ﺟﺬب ﮐﻨﻨﺪﮔﯽ، absorbance ﺟﺎﯾﮕﺎﻩ اﯼ.،ﭘﯽ. A,P site درﺁﺷﺎﻣﻨﺪﮔﯽ، اﺁ (aa (amino acid درﮐﺸﻴﺪﮔﯽ، ﻗﺎﺑﻠﻴﺖ ﺟﺬب اﯼ.اﯼ.ﺟﯽ. AAG (= acid ﺟﺬب، ﺟﺬب ﺳﻄﺤﯽ، alpha-glucosidase) absorption درﺁﺷﺎﻣﯽ، درﮐﺸﯽ، درون اﯼ.اﯼ.ﺗﯽ. (AAT (= alpha-antitrypsin ﺟﺬﺑﯽ اﯼ.اﯼ.وﯼ. AAV (= adeno-associated ﺁﺑﺰﯾﻢ، اﺑﺰاﯾﻢ virus) abzyme ﺁ.ث.، اﯼ.ﺳﯽ. (AC (= adenyl cyclase اﯼ.ﺑﯽ. (ab (= antibody ﺧﺎر ﺳﺮﮎ acanthella ﭘﻴﺸﺒﻴﻨﯽ ژن اب اﯾﻨﻴﺘﻴﻮ ab initio gene prediction ﺧﺎر ﺳﺮان acanthocephala اﻧﺘﻘﺎل دهﻨﺪﻩ اﯼ.ﺑﯽ.ﺳﯽ. ABC transporter =) acanthocephalan ﺟﻮرﻩ/وارﯾﺘﻪ/رﻗﻢ/ﺳﻮﯾﻪ Abelson strain of murine (acanthocephalus (ﻣﺮﺑﻮط ﺑﻪ ﺟﻮﻧﺪﮔﺎن) ﺧﺎر ﺳﺮ acanthocephalus ﺁﺑﻠﺴﻮﻧﯽ ﺧﺎر ﺳﺮﭼﻪ acanthor ﮐﺞ راهﯽ aberration ﺑﯽ ﻗﻠﺒﯽ ﻣﺎدر زاد acardia ﺗﻮﻟﻴﺪ ﺧﻮد ﺑﺨﻮد، ﺗﻮﻟﻴﺪ ﻣﺜﻞ abiogenesis ﺑﯽ ﻗﻠﺐ ﻣﺎدر زاد acardiac ﺧﻮد ﺑﻪ ﺧﻮدﯼ، ﻧﺎزﯾﺴﺖ (acardiacus (=acardiac زاﯾﯽ (acardius (=acardiac ﻏﻴﺮ ﺁﻟﯽ، ﻏﻴﺮ زﻧﺪﻩ، ﻧﺎزﯾﻮا abiotic ﮐﻨﻪ زدﮔﯽ acariasis ﺗﻨﺶ ﻋﻮاﻣﻞ ﻓﻴﺰﯾﮑﯽ ﻣﺤﻴﻂ، abiotic stress ﮐﻨﻪ ﺳﺎﻧﺎن acarina ﺗﻨﺶ اﺑﻴﻮﺗﻴﮏ ﮐﻨﻪ ﺳﺎن acarine اﯼ.ﺑﯽ.ال abl (=Abelson strain of ﮐﻨﻪ زدﮔﯽ (murine) acarinosis (= acariasis ﮐﻨﻪ زدﮔﯽ ﭘﻮﺳﺖ acarodermatitis اﯼ.ﺑﯽ.ال. ABL (=Abelson strain of ﮐﻨﻪ ﺗﺮﺳﯽ murine) acarophobia ﺑﻠﻮغ ﺷﺘﺎﺑﺪار/ﺗﺴﺮﯾﻊ ﺷﺪﻩ accelerated maturation ﻓﺮﺳﺎب، ﺳﺎﯾﺶ، ذوب ﯾﺦ ablation ﮔﻠﻮﺑﻮﻟﻴﻦ ﺷﺘﺎﺑﺪهﻨﺪﻩ/ﺗﺴﺮﯾﻊ accelerator globulin اﯼ.ﺑﯽ.او.
    [Show full text]
  • How Bacillus Thuringiensishas
    Review TRENDS in Genetics Vol.17 No.4 April 2001 193 How Bacillus thuringiensis has evolved specific toxins to colonize the insect world Ruud A. de Maagd, Alejandra Bravo and Neil Crickmore Bacillus thuringiensis is a bacterium of great agronomic and scientific interest. between different strains has been observed both in a Together the subspecies of this bacterium colonize and kill a large variety of soil environment and within insects2. host insects and even nematodes, but each strain does so with a high degree of Individual Cry toxins have a defined spectrum of specificity. This is mainly determined by the arsenal of crystal proteins that the insecticidal activity, usually restricted to a few bacterium produces during sporulation. Here we describe the properties of species within one particular order of insects. To date, these toxin proteins and the current knowledge of the basis for their specificity. toxins for insect species in the orders Lepidoptera Assessment of phylogenetic relationships of the three domains of the active (butterflies and moths), Diptera (flies and toxin and experimental results indicate how sequence divergence in mosquitoes), Coleoptera (beetles and weevils) and combination with domain swapping by homologous recombination might Hymenoptera (wasps and bees) have been identified. have caused this extensive range of specificities. A small minority of crystal toxins shows activity against non-insect species such as nematodes3. A few Bacillus thuringiensis (Bt) is an endospore-forming toxins have an activity spectrum that spans two or bacterium characterized by the presence of a protein three insect orders – most notably Cry1Ba, which is crystal within the cytoplasm of the sporulating cell active against larvae of moths, flies and beetles4.
    [Show full text]
  • Role of Toxin Activation on Binding and Pore Formation Activity of the Bacillus Thuringiensis Cry3 Toxins in Membranes of Leptinotarsa Decemlineata (Say)
    Biochimica et Biophysica Acta 1660 (2004) 99–105 www.bba-direct.com Role of toxin activation on binding and pore formation activity of the Bacillus thuringiensis Cry3 toxins in membranes of Leptinotarsa decemlineata (Say) C. Rausella,1, I. Garcı´a-Roblesb,1,J.Sa´ncheza, C. Mun˜oz-Garaya, A.C. Martı´nez-Ramı´rezb, M.D. Realb, A. Bravoa,* a Instituto de Biotecnologı´a, Universidad Nacional Auto´noma de Me´xico, Ap. Postal 510-3, Cuernavaca 62250, Morelos, Mexico b Facultad de Ciencias Biolo´gicas, Universidad de Valencia, Dr. Moliner 50, Burjassot 46100, Valencia, Spain Received 29 July 2003; received in revised form 5 November 2003; accepted 7 November 2003 Abstract Binding and pore formation constitute key steps in the mode of action of Bacillus thuringiensis y-endotoxins. In this work, we present a comparative analysis of toxin-binding capacities of proteolytically processed Cry3A, Cry3B and Cry3C toxins to brush border membranes (BBMV) of the Colorado potato beetle Leptinotarsa decemlineata (CPB), a major potato coleopteran-insect pest. Competition experiments showed that the three Cry3 proteolytically activated toxins share a common binding site. Also heterologous competition experiments showed that Cry3Aa and Cry3Ca toxins have an extra binding site that is not shared with Cry3Ba toxin. The pore formation activity of the three different Cry3 toxins is analysed. High pore-formation activities were observed in Cry3 toxins obtained by proteolytical activation with CPB BBMV in contrast to toxins activated with either trypsin or chymotrypsin proteases. The pore-formation activity correlated with the formation of soluble oligomeric structures. Our data support that, similarly to the Cry1A toxins, the Cry3 oligomer is formed after receptor binding and before membrane insertion, forming a pre-pore structure that is insertion-competent.
    [Show full text]
  • Bt Toxin Modification for Enhanced Efficacy
    Toxins 2014, 6, 3005-3027; doi:10.3390/toxins6103005 OPEN ACCESS toxins ISSN 2072-6651 www.mdpi.com/journal/toxins Review Bt Toxin Modification for Enhanced Efficacy Benjamin R. Deist 1, Michael A. Rausch 1, Maria Teresa Fernandez-Luna 1, Michael J. Adang 2,3 and Bryony C. Bonning 1,* 1 Department of Entomology, Iowa State University, Ames, IA 50011, USA; E-Mails: [email protected] (B.R.D.); [email protected] (M.A.R.); [email protected] (M.T.F.-L.) 2 Departments of Entomology, University of Georgia, Athens, GA 30602, USA; E-Mail: [email protected] 3 Department of Biochemistry and Molecular Biology, University of Georgia, Athens, GA 30602, USA * Author to whom correspondence should be addressed; E-Mail: [email protected]; Tel.: +1-515-294-1989; Fax: +1-515-294-2995. External Editor: Anne-Brit Kolstø Received: 2 May 2014; in revised form: 28 September 2014 / Accepted: 29 September 2014 / Published: 22 October 2014 Abstract: Insect-specific toxins derived from Bacillus thuringiensis (Bt) provide a valuable resource for pest suppression. Here we review the different strategies that have been employed to enhance toxicity against specific target species including those that have evolved resistance to Bt, or to modify the host range of Bt crystal (Cry) and cytolytic (Cyt) toxins. These strategies include toxin truncation, modification of protease cleavage sites, domain swapping, site-directed mutagenesis, peptide addition, and phage display screens for mutated toxins with enhanced activity. Toxin optimization provides a useful approach to extend the utility of these proteins for suppression of pests that exhibit low susceptibility to native Bt toxins, and to overcome field resistance.
    [Show full text]
  • Evolução Do Veneno Em Cnidários Baseada Em Dados De Genomas E Proteomas
    Adrian Jose Jaimes Becerra Evolução do veneno em cnidários baseada em dados de genomas e proteomas Venom evolution in cnidarians based on genomes and proteomes data São Paulo 2015 i Adrian Jose Jaimes Becerra Evolução do veneno em cnidários baseada em dados de genomas e proteomas Venom evolution in cnidarians based on genomes and proteomes data Dissertação apresentada ao Instituto de Biociências da Universidade de São Paulo, para a obtenção de Título de Mestre em Ciências, na Área de Zoologia. Orientador: Prof. Dr. Antonio C. Marques São Paulo 2015 ii Jaimes-Becerra, Adrian J. Evolução do veneno em cnidários baseada em dados de genomas e proteomas. 103 + VI páginas Dissertação (Mestrado) - Instituto de Biociências da Universidade de São Paulo. Departamento de Zoologia. 1. Veneno; 2. Evolução; 3. Proteoma. 4. Genoma I. Universidade de São Paulo. Instituto de Biociências. Departamento de Zoologia. Comissão Julgadora Prof(a) Dr(a) Prof(a) Dr(a) Prof. Dr. Antonio Carlos Marques iii Agradecimentos Eu gostaria de agradecer ao meu orientador Antonio C. Marques, pela confiança desde o primeiro dia e pela ajuda tanto pessoal como profissional durantes os dois anos de mestrado. Obrigado por todo. Ao CAPES, pela bolsa de mestrado concedida. Ao FAPESP pelo apoio financeiro durante minha estadia em Londres. Ao Instituto de Biociências da Universidade de São Paulo, pela estrutura oferecida durante a execução desde estudo. Ao Dr. Paul F. Long pelas conversas, por toda sua ajuda, por acreditar no meu trabalho. Aos colegas e amigos de Laboratório de Evolução Marinha (LEM), Jimena Garcia, María Mendoza, Thaís Miranda, Amanda Cunha, Karla Paresque, Marina Fernández, Fernanda Miyamura e Lucília Miranda, pela amizade, dicas e ajuda em tudo e por me fazer sentir em casa, muito obrigado mesmo! Aos meus amigos fora do laboratório, John, Soly, Chucho, Camila, Faride, Cesar, Angela, Camilo, Isa, Nathalia, Susana e Steffania, pelo apoio e por me fazer sentir em casa.
    [Show full text]
  • The First Cry2ac-Type Protein Toxic to Helicoverpa Armigera: Cloning and Overexpression of Cry2ac7 Gene from SBS-BT1 Strain of Bacillus Thuringiensis
    toxins Article The First Cry2Ac-Type Protein Toxic to Helicoverpa armigera: Cloning and Overexpression of Cry2ac7 Gene from SBS-BT1 Strain of Bacillus thuringiensis Faiza Saleem 1,2 and Abdul Rauf Shakoori 1,* 1 School of Biological Sciences, University of the Punjab, Quaid-i-Azam Campus, Lahore 54590, Pakistan; [email protected] 2 Department of Biotechnology, Lahore College for Women University, Lahore 54590, Pakistan * Correspondence: [email protected] or [email protected] Academic Editor: Anne-Brit Kolstø Received: 26 August 2017; Accepted: 27 October 2017; Published: 3 November 2017 Abstract: The Cry (crystal) proteins from Bacillus thuringiensis are known to have toxicity against a variety of insects and have been exploited to control insect pests through transgenic plants and biopesticides. B. thuringiensis SBS BT-1 carrying the cry2 genes was isolated from soil samples in Pakistan. The 2-kb full length cry2Ac gene was cloned, sequenced, and submitted to the EMBL DNA database (Accession No. AM292031). For expression analysis, Escherichia coli DH5α was transformed with the fragment sub-cloned in pET22b expression vector using NdeI and HindIII restriction sites, and later confirmed by restriction endonuclease analysis. To assess the toxicity of Cry2Ac7 protein against lepidopteran and dipteran insects, BL21 (codon plus) strain of E. coli was further transformed with the recombinant plasmid. The 65-kDa protein was expressed in the form of inclusion bodies up to 180 OD units per liter of the medium. Inclusions were washed with a buffer containing 1.5% Triton-X 100 and >90% pure Cry2Ac7 was obtained. The inclusion bodies were dissolved in 50 mM K2CO3 (pH 11.5), dialyzed, and freeze-dried.
    [Show full text]
  • Cooperation and Bacterial Pathogenicity: an Approach to Social Evolution C Alfonso Molina1,3* and Susana Vilchez2
    Molina and Vilchez Revista Chilena de Historia Natural 2014, 87:14 http://www.revchilhistnat.com/content/87/1/14 REVIEW Open Access Cooperation and bacterial pathogenicity: an approach to social evolution C Alfonso Molina1,3* and Susana Vilchez2 Abstract Kin selection could provide an explanation for social behavior in bacteria. The production of public goods such as extracellular molecules is metabolically costly for bacteria but could help them to exploit nutrients or invade a host. Some bacterial cells called social cheaters do not produce public goods; however, they take advantage of these extracellular molecules. In this review, the relationships between social behavior, cooperation, and evolution of bacterial pathogenicity are analyzed. This paper also examines the role of horizontal transfer of genes encoding for virulence factors and how the movement of mobile genetic elements would influence the pathogenicity and social relationships. Moreover, the link between ecological relationships and evolution in entomopathogenic bacteria, focusing on Bacillus thuringiensis is considered. Finally, the findings obtained with B. thuringiensis are extrapolated on Bacillus pumilus 15.1, an entomopathogenic strain whose pathogenicity is not understood yet. Keywords: Cooperation; Evolution; Pathogenicity; Public goods; Sociability Introduction (Brown 1999), adhesive polymers (Rainey and Rainey The social relationships of microorganisms are based on 2003), or siderophores (West and Buckling 2003), amongst a wide range of extracellular actions produced by indi- others. This social behavior is, from a metabolic point of vidual cells, which can affect the reproductive efficiency view, costly for the individual cells, although it is beneficial of other nearby cells. The production of molecules such for the group.
    [Show full text]
  • Membrane-Active Derivatives of the Frog Skin Peptide Esculentin-1 Against Relevant Human Pathogens
    DOTTORATO DI RICERCA IN BIOCHIMICA CICLO XXVI (A.A. 2010-2013) Membrane-active derivatives of the frog skin peptide Esculentin-1 against relevant human pathogens Docente guida Coordinatore Prof.ssa Maria Luisa MANGONI Prof. Francesco MALATESTA Prof.ssa Donatella BARRA Dottorando Vincenzo LUCA Dicembre 2013 DOTTORATO DI RICERCA IN BIOCHIMICA CICLO XXVI (A.A. 2010-2013) Membrane-active derivatives of the frog skin peptide Esculentin-1 against relevant human pathogens Dottorando Vincenzo LUCA Docente guida Coordinatore Prof.ssa Maria Luisa MANGONI Prof. Francesco MALATESTA Prof.ssa Donatella BARRA Dicembre 2013 To my brother INDEX INDEX List of papers relevant for this thesis 1 List of other papers 1 1. INTRODUCTION 3 1.1 Antimicrobial peptides and innate immunity 3 1.2 AMPs: structural features and mechanism of action 6 1.3 AMPs and resistance 13 1.4 Amphibian AMPs 16 1.5 The esculentin family 17 1.6 The 1-18 fragment of esculentin-1b, Esc(1-18) 19 1.7 The 1-21 fragment of esculentin-1a, Esc(1-21) 20 1.8 Candida albicans pathogen 21 1.9 Pseudomonas aeruginosa pathogen 23 1.10 Caenorhabditis elegans: a simple infection model 24 2. AIM OF THE STUDY 27 3. MATERIALS AND METHODS 28 3.1 Materials 28 3.2 Microorganisms and nematode strains 28 3.3 Circular dichroism (CD) analysis 29 3.4 Hemolytic activity 29 I INDEX 3.5 Determination of the minimal peptide concentration inhibiting the growth of free-living microorganisms 30 3.6 Anti-biofilm activity 31 3.7 Time-killing of free-living microorganisms (yeasts and bacteria) 33 3.8 Peptide’s effect on the viability of hyphae 34 3.9 Inhibition of hyphal development 35 3.10 Membrane permeabilization of C.
    [Show full text]
  • (12) United States Patent (10) Patent No.: US 7,846.445 B2 Schellenberger Et Al
    USOO7846445B2 (12) United States Patent (10) Patent No.: US 7,846.445 B2 Schellenberger et al. (45) Date of Patent: Dec. 7, 2010 (54) METHODS FOR PRODUCTION OF 5,176,502 A 1/1993 Sanderson et al. UNSTRUCTURED RECOMBINANT 5, 186,938 A 2/1993 Sablotsky et al. POLYMERS AND USES THEREOF 5,215,680 A 6/1993 D'arrigo 5,223,409 A 6/1993 Ladner et al. (75) Inventors: Volker Schellenberger, Palo Alto, CA 5,270,176 A * 12/1993 Dorschug et al. .......... 435/69.7 (US); Willem P. Stemmer, Los Gatos, 5,298,022 A 3, 1994 Bernardi CA (US); Chia-wei Wang, Santa Clara, 5,318.540 A 6, 1994 Athayde et al. CA (US); Michael D. Schole, Mountain 5,407,609 A 4/1995 Tice et al. View, CA (US); Mikhail Popkov, San 2. 3. E. 1 Diego, CA (US); Nathaniel C. Gordon, - - I oiszwillo et al. Campbell, CA (US); Andreas Crameri, 5,573,776 A 11, 1996 Harrison et al. Los Altos Hills, CA (US) 5,578,709 A 11/1996 WoiSZwillo s 5,599.907 A * 2/1997 Anderson et al. ........... 530,385 5,660,848 A 8, 1997 Moo-Youn (73) Assignee: suioperating, Inc., Mountain 5,756,115 A 5, 1998 NSW et al. iew, CA (US) 5,874,104 A 2/1999 Adler-moore et al. 5,916,588 A 6, 1999 P tal. (*) Notice: Subject to any disclaimer, the term of this 5,942,252 A 8, 1999 p al patent is extended or adjusted under 35 5,965,156 A 10, 1999 Profitt et al.
    [Show full text]
  • Association of Orfx-P47 Gene Clusters with Toxin Genes in Clostridial and Non-Clostridial Bacterial Species
    toxins Article Looking for the X Factor in Bacterial Pathogenesis: Association of orfX-p47 Gene Clusters with Toxin Genes in Clostridial and Non-Clostridial Bacterial Species Maria B. Nowakowska, François P. Douillard and Miia Lindström * Department of Food Hygiene and Environmental Health, Faculty of Veterinary Medicine, University of Helsinki, 00014 Helsinki, Finland; maria.nowakowska@helsinki.fi (M.B.N.); francois.douillard@helsinki.fi (F.P.D.) * Correspondence: miia.lindstrom@helsinki.fi Received: 9 December 2019; Accepted: 29 December 2019; Published: 31 December 2019 Abstract: The botulinum neurotoxin (BoNT) has been extensively researched over the years in regard to its structure, mode of action, and applications. Nevertheless, the biological roles of four proteins encoded from a number of BoNT gene clusters, i.e., OrfX1-3 and P47, are unknown. Here, we investigated the diversity of orfX-p47 gene clusters using in silico analytical tools. We show that the orfX-p47 cluster was not only present in the genomes of BoNT-producing bacteria but also in a substantially wider range of bacterial species across the bacterial phylogenetic tree. Remarkably, the orfX-p47 cluster was consistently located in proximity to genes coding for various toxins, suggesting that OrfX1-3 and P47 may have a conserved function related to toxinogenesis and/or pathogenesis, regardless of the toxin produced by the bacterium. Our work also led to the identification of a putative novel BoNT-like toxin gene cluster in a Bacillus isolate. This gene cluster shares striking similarities to the BoNT cluster, encoding a bont/ntnh-like gene and orfX-p47, but also differs from it markedly, displaying additional genes putatively encoding the components of a polymorphic ABC toxin complex.
    [Show full text]
  • Bacillus Thuringiensis - Derived Insect Control Proteins
    Unclassified ENV/JM/MONO(2007)14 Organisation de Coopération et de Développement Economiques Organisation for Economic Co-operation and Development 26-Jul-2007 ___________________________________________________________________________________________ English - Or. English ENVIRONMENT DIRECTORATE JOINT MEETING OF THE CHEMICALS COMMITTEE AND Unclassified ENV/JM/MONO(2007)14 THE WORKING PARTY ON CHEMICALS, PESTICIDES AND BIOTECHNOLOGY Cancels & replaces the same document of 19 July 2007 SERIES ON HARMONISATION OF REGULATORY OVERSIGHT IN BIOTECHNOLOGY Number 42 CONSENSUS DOCUMENT ON SAFETY INFORMATION ON TRANSGENIC PLANTS EXPRESSING BACILLUS THURINGIENSIS - DERIVED INSECT CONTROL PROTEINS This document has been cancelled and replaced to correct mistakes in formatting due to technical problems. English - Or. English JT03230592 Document complet disponible sur OLIS dans son format d'origine Complete document available on OLIS in its original format ENV/JM/MONO(2007)14 Also published in the Series on Harmonisation of Regulatory Oversight in Biotechnology: No. 1, Commercialisation of Agricultural Products Derived through Modern Biotechnology: Survey Results (1995) No. 2, Analysis of Information Elements Used in the Assessment of Certain Products of Modern Biotechnology (1995) No. 3, Report of the OECD Workshop on the Commercialisation of Agricultural Products Derived through Modern Biotechnology (1995) No. 4, Industrial Products of Modern Biotechnology Intended for Release to the Environment: The Proceedings of the Fribourg Workshop (1996) No. 5, Consensus Document on General Information concerning the Biosafety of Crop Plants Made Virus Resistant through Coat Protein Gene-Mediated Protection (1996) No. 6, Consensus Document on Information Used in the Assessment of Environmental Applications Involving Pseudomonas (1997) No. 7, Consensus Document on the Biology of Brassica napus L. (Oilseed Rape) (1997) No.
    [Show full text]