Polyclonal Antibody to APC11 / ANAPC11 - Serum

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R1503 Polyclonal Antibody to APC11 / ANAPC11 - Serum

Alternate names: Anaphase-promoting complex subunit 11, Cyclosome subunit 11, HSPC214, Hepatocellular carcinoma-associated RING finger protein Quantity: 0.1 ml Concentration: 85 mg/ml (by Refractometry) Background: APC11 is also known as Anaphase promoting complex subunit 11, APC11, Cyclosome subunit 11, Hepatocellular carcinoma associated RING finger protein, and HSPC214. APC11 is a component of the anaphase promoting complex/cyclosome (APC/C), a cell cycle-regulated E3 ubiquitin ligase that controls progression through mitosis and the G1 phase of the cell cycle. APC11 may function to recruit the E2 ubiquitin-conjugating enzymes to the complex. APC11 interacts with the cullin domain of ANAPC2 and also interacts with UBE2D2. APC11 shows both a cytoplasmic and nuclear localization. APC11 is expressed at high levels in skeletal muscle and heart; in moderate levels in brain, kidney, and liver; and at low levels in colon, thymus, spleen, small intestine, placenta, lung and peripheral blood leukocyte. APC11 is a member of the RING-type zinc finger family and is auto-ubiquitinylated. Uniprot ID: Q9NYG5 NCBI: NP_001002244.1 GeneID: 51529 Host: Rabbit Immunogen: This APC11 antibody was prepared from whole rabbit serum produced by repeated immunizations with a synthetic peptide corresponding to amino acids 76-84 of Human APC11 (C-terminal) coupled to KLH. AA Sequence: Human APC11 (84 aa 9.8 kDa): 1 mkvkikcwng vatwlwvand encgicrmaf ngccpdckvp gddcplvwgq cshcfhmhci 61 lkwlhaqqvq qhcpmcrqew kfke

Format: State: Liquid (sterile filtered) containing 0.01% (w/v) Sodium Azide as preservative. Purification: Delipidation and defibrination. Applications: Western blot (1:500-1:1,000). ELISA (1:2,000-1:10,000). Immunoprecipitation: The antibody immunoprecipitates in vitro translated protein and protein from overexpressing cell lysates (using HeLa and NIH-3T3, and others). Coimmunoprecipitation of related proteins (APC2) does occur. A 9.8 kDa band corresponding to human APC11 is detected. Most cell lines or tissues expressing APC11 can be used as a positive control. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This product is monospecific antiserum processed by delipidation and defibrination followed by sterile filtration. This antibody reacts with human and mouse APC11. Cross reactivity may also occur

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20140808 1 / 3 R1503: Polyclonal Antibody to APC11 / ANAPC11 - Serum

with APC11 from other sources. Sufficient sequence differences exist to suggest that this antibody would not react with other RING box proteins such as ROC1 and ROC2. Storage: Store vial at -20°C prior to opening. Aliquot contents and freeze at -20°C or below for extended storage. Centrifuge product if not completely clear after standing at room temperature. This product is stable for one month at 2-8°C as an undiluted liquid. Dilute only prior to immediate use. Avoid cycles of freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Ohta T, Michel JJ, Xiong Y. Association with cullin partners protects ROC proteins from proteasome-dependent degradation. Oncogene. 1999 Nov 18;18(48):6758-66. PubMed PMID: 10597284. 2. Furukawa M, Ohta T, Xiong Y. Activation of UBC5 ubiquitin-conjugating enzyme by the RING finger of ROC1 and assembly of active ubiquitin ligases by all cullins. J Biol Chem. 2002 May 3;277(18):15758-65. Epub 2002 Feb 22. PubMed PMID: 11861641. 3. Jentsch S, Pyrowolakis G. Ubiquitin and its kin: how close are the family ties? Trends Cell Biol. 2000 Aug;10(8):335-42. PubMed PMID: 10884686. 4. Chan AH, Lee SM, Chim SS, Kok LD, Waye MM, Lee CY, et al. Molecular cloning and characterization of a RING-H2 finger protein, ANAPC11, the human homolog of yeast Apc11p. J Cell Biochem. 2001 Aug 1-9;83(2):249-58. PubMed PMID: 11573242. 5. Gmachl M, Gieffers C, Podtelejnikov AV, Mann M, Peters JM. The RING-H2 finger protein APC11 and the E2 enzyme UBC4 are sufficient to ubiquitinate substrates of the anaphase-promoting complex. Proc Natl Acad Sci U S A. 2000 Aug 1;97(16):8973-8. PubMed PMID: 10922056. 6. Tang Z, Li B, Bharadwaj R, Zhu H, Ozkan E, Hakala K, et al. APC2 Cullin protein and APC11 RING protein comprise the minimal ubiquitin ligase module of the anaphase- promoting complex. Mol Biol Cell. 2001 Dec;12(12):3839-51. PubMed PMID: 11739784.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20140808 2 / 3 R1503: Polyclonal Antibody to APC11 / ANAPC11 - Serum

Pictures: Figure 1. Conjugation pathways for ubiquitin and ubiquitin-like modifiers (UBLs). Most modifiers mature by proteolytic processing from inactive precursors (a; amino acid). Arrowheads point to the cleavage sites. Ubiquitin is expressed either as polyubiquitin or as a fusion with ribosomal proteins. Conjugation requires activating (E1) and conjugating (E2) enzymes that form thiolesters (S) with the mdifiers. Modification of cullins by RUB involves SCF(SKP1/cullin-1/F-box protein) /CBC(cullin-2/elongin B/elongin C)-like E3 enzymes that are also involved in ubiquitination. In contrast to ubiquitin, the UBLs do not seem to form multi-UBL chains. UCRP(ISG15) resembles two ubiquitin moieties linked headto-tail. Whether HUB1 functions as a modifier is currently unclear. APG12 and URM1 are distinct from the other modifiers because they are unrelated in sequence to ubiquitin. Data contributed by S.Jentsch, see reference 3.

For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. MS/20140808 3 / 3