Recombinant Human Cytosolic Sulfotransferase 1E1/SULT1E1 Protein Catalog Number: ATGP0284

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Recombinant human Cytosolic Sulfotransferase 1E1/SULT1E1 protein Catalog Number: ATGP0284 PRODUCT INPORMATION Expression system E.coli Domain 1-294aa UniProt No. P49888 NCBI Accession No. AAH27956 Alternative Names Sulfotransferase family 1E estrogen-preferring member 1, SuLT1E1, EST, EST-1, STE, Estrogen-preferring EST-1, Sulfotransferase family 1E, estrogen-preferring, member 1 EST1, estrone sulfotransferase, STE, Sulfotransferase estrogen preferring. PRODUCT SPECIFICATION Molecular Weight 36.1 kDa (302aa) confirmed by MALDI-TOF Concentration 1mg/ml (determined by Bradford assay) Formulation Liquid in. 20mM Tris-HCl buffer (pH 8.0) containing 20% glycerol Purity > 95% by SDS-PAGE Endotoxin level < 1 EU per 1ug of protein (determined by LAL method) Tag His-Tag Application SDS-PAGE Storage Condition Can be stored at +2C to +8C for 1 week. For long term storage, aliquot and store at -20C to -80C. Avoid repeated freezing and thawing cycles. BACKGROUND Description Sulfotransferase enzymes catalyze the sulfate conjugation of many hormones, neurotransmitters, drugs, and xenobiotic compounds. These cytosolic enzymes are different in their tissue distributions and substrate 1 Recombinant human Cytosolic Sulfotransferase 1E1/SULT1E1 protein Catalog Number: ATGP0284 specificities. Estrogen sulfotransferase may control the level of the estrogen receptor by sulfurylating free estradiol. Recombinant SuLT1E1, fused to His-tag at C-terminus, was expressed in E. coli and was purified by conventional chromatography. Amino acid Sequence MNSELDYYEK FEEVHGILMY KDFVKYWDNV EAFQARPDDL VIATYPKSGT TWVSEIVYMI YKEGDVEKCK EDVIFNRIPF LECRKENLMN GVKQLDEMNS PRIVKTHLPP ELLPASFWEK DCKIIYLCRN AKDVAVSFYY FFLMVAGHPN PGSLPEFVEK FMQGQVPYGS WYKHVKSWWE KGKSPRVLFL FYEDLKEDIR KEVIKLIHFL ERKPSEELVD RIIHHTSFQE MKNNPSTNYT TLPDEIMNQK LSPFMRKGIT GDWKNHFTVA LNEKFDKHYE QQMKESTLKF RTEILEHHHH HH General References Maiti S., et al. (2007) Biochem Pharmacol. 73(9): 1474-81. Adjei AA., et al. (2003) Br.J Phamacol. 139(8): 1373-82. DATA SDS-PAGE 3ug by SDS-PAGE under reducing condition and visualized by coomassie blue stain. 2.

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