Polyclonal Antibody to ZFY (Center) - Aff - Purified

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Polyclonal Antibody to ZFY (Center) - Aff - Purified

OriGene Technologies, Inc. OriGene Technologies GmbH 9620 Medical Center Drive, Ste 200 Schillerstr. 5 Rockville, MD 20850 32052 Herford UNITED STATES GERMANY Phone: +1-888-267-4436 Phone: +49-5221-34606-0 Fax: +1-301-340-8606 Fax: +49-5221-34606-11 [email protected] [email protected] AP54650PU-N Polyclonal Antibody to ZFY (Center) - Aff - Purified Alternate names: Zinc finger Y-chromosomal protein Quantity: 0.4 ml Concentration: lot specific Background: This gene encodes a zinc finger-containing protein that may function as a transcription factor. This gene was once a candidate gene for the testis-determining factor (TDF) and was erroneously referred to as TDF. Uniprot ID: P08048 NCBI: NP_001138747 GeneID: 7544 Host / Isotype: Rabbit / Ig Immunogen: KLH conjugated synthetic peptide between 322-351 amino acids from the Central region of human ZFY Format: State: Liquid Ig fraction Purification: Protein A column followed by peptide affinity purification Buffer System: PBS with 0.09% (W/V) sodium azide Applications: ELISA: 1:1;000. Western blot: 1:100~500. Other applications not tested. Optimal dilutions are dependent on conditions and should be determined by the user. Specificity: This antibody detects ZFY (Center). Species Reactivity: Tested: Human Add. Information: Molecular Weight: 90505 Da Storage: Store at 2 - 8 °C for up to six months or (in aliquots) at -20 °C for longer. Avoid repeated freezing and thawing. Shelf life: one year from despatch. General Readings: 1. Skaletsky, H., et al. Nature 423(6942):825-837(2003). 2. Merchant-Larios, H., et al. Arch. Med. Res. 32(6):553-558(2001). 3. Erlandsson, R., et al. Mol. Biol. Evol. 17(5):804-812(2000). 4. Mittwoch, U. Cytogenet. Cell Genet. 91 (1-4), 186-191 (2000). 5. Tricoli, J.V., et al. Genes Chromosomes Cancer 6(2):65-72(1993). For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 1 / 2 AP54650PU-N: Polyclonal Antibody to ZFY (Center) - Aff - Purified Pictures: ZFY Antibody (Center) (AP54650PU-N) western blot analysis in A549 cell line lysates (35 µg/lane). This demonstrates the ZFY antibody detected the ZFY protein (arrow). For research and in vitro use only. Not for diagnostic or therapeutic work. Material Safety Datasheets are available at www.acris-antibodies.com or on request. OG/20130805 2 / 2 Powered by TCPDF (www.tcpdf.org).

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Zfyl Encodes a Nuclear Sequence-Specific DNA Binding
View metadata, citation and similar papers at core.ac.uk brought to you byCORE provided by Elsevier - Publisher Connector FEBS 15213 FEBS Letters 360 (1995) 315-319 Zfyl encodes a nuclear sequence-specific DNA binding protein Pamela Taylor-Harris, Sally Swift, Alan Ashworth* CRC Centre of Cell and Molecular Biology, Chester Beatty Laboratories, The Institute of Cancer Research, 237 Fulham Road, London, SW3 6JB, UK Received 19 January 1995; revised version received 3 February 1995 sively to RNA and have functions in RNA storage or processes Abstract Zfyl is a mouse Y chromosomal gene encoding a zinc such as splicing. finger protein which is thought to have some function during In order to analyse the properties of the ZFY family of zinc spermatogenesis. Here we show that, when introduced into tissue finger proteins, we have raised specific antibodies to murine culture cells, Zfyl is targeted to the nucleus. Two independent signals are present within the protein for nuclear localization. Zfyl. These have been used to demonstrate that Zfyl protein This nuclear Zfyl protein is able to bind strongly to DNA-ceHu- localizes to the nucleus of transfected cells and to identify spe- lose and, using site-selection assays, we have identified specific cific DNA binding sites for Zfyl, suggesting a role for this Zfyl DNA binding sites. Taken together these results suggest protein as a transcription factor regulating gene expression that Zfyl is a nuclear-located sequence-specific DNA binding during spermatogenesis. protein which functions during spermatogenesis. Key words: Spermatogenesis; Zinc finger protein; 2. Materials and methods Y chromosome; Nucleus; DNA binding; Transcription factor 2.1.
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