Author Manuscript Published OnlineFirst on August 8, 2012; DOI: 10.1158/1078-0432.CCR-12-0543 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. Alternative cleavage and polyadenylation during colorectal cancer development Adam R. Morris1#, Arnold Bos1#, Begoña Diosdado2, Koos Rooijers1, Ran Elkon1, Anne S. Bolijn2, Beatriz Carvalho2, Gerrit A. Meijer2, and Reuven Agami1,3$ 1. Division of Gene Regulation, The Netherlands Cancer Institute, Plesmanlaan 121, 1066 CX, Amsterdam, The Netherlands 2. Department of Pathology VU medical centre, De Boelelaan 1117, 1081HV Amsterdam, The Netherlands 3. The Centre for Biomedical Genetics, The Netherlands Running Title: Alternative polyadenylation in colorectal cancer Keywords: Alternative cleavage and polyadenylation, colorectal cancer, next generation sequencing, Adenoma, carcinoma Conflicts of interest: none Word count: 4055 Tables: 2; Figures: 3 # These authors contributed equally to this work $Email:
[email protected] Tel. +31(0)20 512 2079 Fax. +31(0)20 512 1999 Downloaded from clincancerres.aacrjournals.org on September 28, 2021. © 2012 American Association for Cancer Research. Author Manuscript Published OnlineFirst on August 8, 2012; DOI: 10.1158/1078-0432.CCR-12-0543 Author manuscripts have been peer reviewed and accepted for publication but have not yet been edited. ABSTRACT Purpose: Alternative cleavage and polyadenylation (APA) of mRNAs is a phenomenon that alters 3'-untranslated region length leading to altered posttranscriptional regulation of gene expression. Changing APA patterns have been shown to result in mis-regulation of genes involved in carcinogenesis, therefore we hypothesized that altered APA contributes to progression of colorectal cancer, and that measurement of APA may lead to discovery of novel biomarkers.