PPIE Antibody Cat. No.: 29-363 PPIE Antibody Specifications HOST SPECIES: Rabbit SPECIES REACTIVITY: Dog, Drosophila, Human, Mouse, Rat, Zebrafish Antibody produced in rabbits immunized with a synthetic peptide corresponding a region IMMUNOGEN: of human PPIE. TESTED APPLICATIONS: ELISA, WB PPIE antibody can be used for detection of PPIE by ELISA at 1:312500. PPIE antibody can APPLICATIONS: be used for detection of PPIE by western blot at 2.5 μg/mL, and HRP conjugated secondary antibody should be diluted 1:50,000 - 100,000. POSITIVE CONTROL: 1) Cat. No. 1205 - Jurkat Cell Lysate PREDICTED MOLECULAR 26 kDa, 33 kDa, 33 kDa WEIGHT: Properties PURIFICATION: Antibody is purified by protein A chromatography method. CLONALITY: Polyclonal CONJUGATE: Unconjugated PHYSICAL STATE: Liquid September 29, 2021 1 https://www.prosci-inc.com/ppie-antibody-29-363.html Purified antibody supplied in 1x PBS buffer with 0.09% (w/v) sodium azide and 2% BUFFER: sucrose. CONCENTRATION: batch dependent For short periods of storage (days) store at 4˚C. For longer periods of storage, store PPIE STORAGE CONDITIONS: antibody at -20˚C. As with any antibody avoid repeat freeze-thaw cycles. Additional Info OFFICIAL SYMBOL: PPIE ALTERNATE NAMES: PPIE, CYP33, CYP-33 ACCESSION NO.: NP_982282 PROTEIN GI NO.: 45439318 GENE ID: 10450 USER NOTE: Optimal dilutions for each application to be determined by the researcher. Background and References PPIE is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activityThe protein encoded by this BACKGROUND: gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities and also exhibit RNA-binding activity. Three alternatively spliced transcript variants encoding distinct isoforms have been observed. REFERENCES: 1) Fair, K., (2001) Mol. Cell. Biol. 21 (10), 3589-3597. ANTIBODIES FOR RESEARCH USE ONLY. For additional information, visit ProSci's Terms & Conditions Page. September 29, 2021 2 https://www.prosci-inc.com/ppie-antibody-29-363.html.