PPIE Antibody Cat. No.: 22-668
PPIE Antibody
Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug PPIE antibody (22-668). Western blot was performed from the immunoprecipitate using PPIE antibody (22-668) at a dilition of 1:1000.
Specifications
HOST SPECIES: Rabbit
SPECIES REACTIVITY: Human, Mouse, Rat
Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of IMMUNOGEN: human PPIE (NP_006103.1).
TESTED APPLICATIONS: IP, WB
WB: ,1:500 - 1:2000 APPLICATIONS: IP: ,1:50 - 1:200
POSITIVE CONTROL: 1) HeLa
2) THP-1
September 30, 2021 1 https://www.prosci-inc.com/ppie-antibody-22-668.html 3) Jurkat
4) HL-60
5) Mouse testis
6) Mouse thymus
PREDICTED MOLECULAR Observed: 33kDa WEIGHT:
Properties
PURIFICATION: Affinity purification
CLONALITY: Polyclonal
ISOTYPE: IgG
CONJUGATE: Unconjugated
PHYSICAL STATE: Liquid
BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.
Additional Info
OFFICIAL SYMBOL: PPIE
ALTERNATE NAMES: PPIE, CYP33, CYP-33
GENE ID: 10450
USER NOTE: Optimal dilutions for each application to be determined by the researcher.
Background and References
The protein encoded by this gene is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of proline imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a BACKGROUND: highly conserved cyclophilin (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and protein folding activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on chromosome 1, has been identified.
ANTIBODIES FOR RESEARCH USE ONLY.
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September 30, 2021 2 https://www.prosci-inc.com/ppie-antibody-22-668.html