PPIE Antibody Cat. No.: 22-668

PPIE Antibody

Immunoprecipitation analysis of 200ug extracts of HeLa cells using 1ug PPIE antibody (22-668). Western blot was performed from the immunoprecipitate using PPIE antibody (22-668) at a dilition of 1:1000.

Specifications

HOST SPECIES: Rabbit

SPECIES REACTIVITY: Human, Mouse, Rat

Recombinant fusion protein containing a sequence corresponding to amino acids 1-301 of IMMUNOGEN: human PPIE (NP_006103.1).

TESTED APPLICATIONS: IP, WB

WB: ,1:500 - 1:2000 APPLICATIONS: IP: ,1:50 - 1:200

POSITIVE CONTROL: 1) HeLa

2) THP-1

September 30, 2021 1 https://www.prosci-inc.com/ppie-antibody-22-668.html 3) Jurkat

4) HL-60

5) Mouse testis

6) Mouse thymus

PREDICTED MOLECULAR Observed: 33kDa WEIGHT:

Properties

PURIFICATION: Affinity purification

CLONALITY: Polyclonal

ISOTYPE: IgG

CONJUGATE: Unconjugated

PHYSICAL STATE: Liquid

BUFFER: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.

STORAGE CONDITIONS: Store at -20˚C. Avoid freeze / thaw cycles.

Additional Info

OFFICIAL SYMBOL: PPIE

ALTERNATE NAMES: PPIE, CYP33, CYP-33

GENE ID: 10450

USER NOTE: Optimal dilutions for each application to be determined by the researcher.

Background and References

The protein encoded by this is a member of the peptidyl-prolyl cis-trans isomerase (PPIase) family. PPIases catalyze the cis-trans isomerization of imidic peptide bonds in oligopeptides and accelerate the folding of proteins. This protein contains a BACKGROUND: highly conserved (CYP) domain as well as an RNA-binding domain. It was shown to possess PPIase and activities, and it also exhibits RNA-binding activity. Alternative splicing results in multiple transcript variants. A related pseudogene, which is also located on 1, has been identified.

ANTIBODIES FOR RESEARCH USE ONLY.

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