Behavioral and Developmental Homeostasis in the Fire Ant
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Journal of Insect Physiology 46 (2000) 933–939 www.elsevier.com/locate/jinsphys Behavioral and developmental homeostasis in the fire ant, Solenopsis invicta Deby Lee Cassill *, Walter R. Tschinkel Department of Biological Science, Florida State University, Tallahassee, FL 32306-3050, USA Received 28 May 1999; accepted 5 October 1999 Abstract The von Bertalanffy rule (1960) predicts that low incubation temperature during larval development will result in larger adult body size. If larval development in social insects followed this rule, then low incubation temperature would induce the development of larger workers and possibly even sexuals. To test this prediction, the effect of incubation temperature on larval development, larval meal size, larval tending and worker recruitment to food in the fire ant, Solenopsis invicta was investigated. Temperatures tested where within the range at which brood remains viable. Contrary to the predictions of the von Bertalanffy rule, worker size was unaffected by incubation temperature, and sexuals were reared at the high rather than the low incubation temperature. Moreover, larval meal size, the rate of larval tending by workers and the total number of workers recruited to food were unaffected by temperature. Mechanisms regulating developmental and behavioral homeostasis were as follows: the duration of larval development and the rate of larval growth changed proportionately with temperature such that the mean and variation of pupal size was unaffected by incubation temperature. Larvae solicited at the same rate, swallowed at the same rate and swallowed for the same duration such that meal size was unaffected by incubation temperature. On the brood pile, fewer workers tended brood at higher incubation temperatures, but worker tempo increased; as a result, brood tending was not adversely affected by incubation temperature. The rate of worker recruitment to food sites outside the nest increased with temperature, but the duration of the recruitment effort decreased such that, over time, the same total number of workers was employed to retrieve food. Incubation humidity was also investigated. When brood chambers were less than 100% humid, workers recruited to food and tended larvae (retrieved, sorted and groomed them), but did not feed larvae. Eventually, larvae died of starvation and were canni- balized. 2000 Elsevier Science Ltd. All rights reserved. Keywords: Compensatory behavior; Caste determination; Nest; Feeding; Trophallaxis; Worker polyphenism; Formicidae 1. Introduction 1991; Gu and Danthanarayana, 1992; Bouletreau-Merle and Sillans, 1996; Arnett and Gotelli, 1999). Insects are ectotherms, thus their rate of growth, time The relationship between body size and incubation to maturity and final adult size may depend upon the temperature for social insects is less well studied. In the temperature of their rearing environment as well as their termite, Coptotermes formosanus, low incubation tem- diet (Slansky and Scriber, 1985; Atkinson, 1994; Atkin- perature produced smaller nymphs (Waller and La Fage, son and Sibly, 1997). The inverse relationship between 1988). In the sweat bee, Halictus ligatus, cool rainy body size and developmental temperature, the von Berta- weather during the breeding season produced smaller lanffy rule (von Bertalanffy, 1960), has been found in body size in workers and alates than warm, dry weather many species of solitary insects (Hogue and Hawkins, (Richards and Packer, 1996). In the ant genus Myrmica, the temperature at which eggs are laid determines whether larvae develop rapidly and become workers or whether they hibernate and retain the potential to * Corresponding author. Tel.: +1-520-621-1151 fax: +1-520-621- become queens (Brian, 1955, 1956, 1963). Hibernating 1150. queen-biased larvae are more likely to succeed in E-mail address: [email protected] (D.L. Cassill). becoming queens when incubated at an intermediate 0022-1910/00/$ - see front matter 2000 Elsevier Science Ltd. All rights reserved. PII: S0022-1910(99)00200-0 934 D.L. Cassill, W.R. Tschinkel / Journal of Insect Physiology 46 (2000) 933–939 temperature than at a low or high temperature (Brian, and maintained at 28°C in constant light for one month. 1973). However, the final size of worker-biased larvae Colonies were fed a diet of tenebrionid beetle larvae and reared early in the breeding season was unaffected by 20% sugar water. For the humidity experiment, year-old incubation temperature (Elmes and Wardlaw, 1983). laboratory-reared colonies were used. These colonies Thus, worker size was independent of incubation tem- were reared from newly-mated, monogyne, Solenopsis perature within the viable range, but adult caste was not. invicta queens collected in Tallahassee, Florida, U.S.A. The fire ant, Solenopsis invicta, is a perennial species during the spring of 1989. Both colony types were main- subjected, not only to diurnal temperature fluctuations, tained by methods similar to those described by Banks et but also to seasonal fluctuations. Nesting in the soil buf- al. (1981). There is no difference in the rates of feeding fers these ants from air temperature extremes. In between laboratory-reared and field-reared larvae addition, the deep and complex nests built by fire ants (Cassill and Tschinkel, 1999b). (Tschinkel, 1998) provide the queen and workers a choice of incubation conditions in which to lay eggs and 2.2. Larval development to rear larvae. Workers move brood up and down tem- perature and moisture gradients several times a day to To examine the effect of temperature on final adult optimize incubation conditions (Potts et al., 1984; Porter caste or size, larvae from eight source colonies were and Tschinkel, 1993). The incubation temperature for combined, sifted to collect small 4th instar larvae (mesh optimal brood development in this species is 32°C with #35; Cassill and Tschinkel, 1995) and divided into a survival range of 25–35°C (Porter, 1988). Because groups of 0.1 g each (ෂ100 individuals). Each larval thousands of larvae and pupae are distributed among group was placed into an experimental nest containing thousands of small brood chambers at any given time 100 workers. The size of experimental colony fragments (Cassill, 1996), many may experience less than optimal were comparable to the size of groups found in subter- incubation conditions. ranean brood chambers in the field (Cassill, 1996). The If worker body size follows the von Bertalanffy rule final number of brood successfully reared was not (1960), variation in incubation temperature induced by determined. nest complexity could explain the observed variation in Experimental brood chambers were circular with a worker body size within a cohort (Tschinkel, 1988). diameter of 2.25 cm and a depth of 0.3 cm, comparable Moreover, temperature could regulate a beneficial trade- to the mean size of subterranean chambers in the field. off between colony size and worker body size. The Two colony fragments were established from a source observed increase in colony size at higher temperatures colony. One of the pair was incubated at 25°C, the low- (Porter, 1988; Porter and Tschinkel, 1993) might be est temperature at which brood can be successfully mediated by the production of smaller workers (Porter reared. The other was incubated at 32°C, the temperature and Tschinkel, 1986). at which optimal colony growth occurs (Porter and Temperature could regulate individual growth and Tschinkel, 1993). Both were fed 20% sugar water, water development directly via a temperature switch (Brian, and thawed tenebrionid larvae ad libitum. Each week for 1956), indirectly via a nutritional switch (Wheeler, 1986) a total of three weeks, a random sample of 20 white or both (Passera, 1974). If polyphenism was regulated pupae (a surrogate for adult size, Porter and Tschinkel, directly by a temperature switch, cool incubation tempera- 1985) was harvested from each fragment (n=16×3). ture could induce a rise in JH levels, initiating major or Pupae were sampled during the time when both groups gyne development. Subsequently, the rate of larval growth were producing abundant pupae. Pupae were weighed would increase causing larvae to signal more often for individually. Pupae with a mass between 1 mg and 4.25 feedings (Cassill and Tschinkel, 1995, 1999a). Alterna- mg were counted as majors; those with a mass Ͻ1mg tively, temperature-delayed development could result in a were counted as minors. These procedures were repli- larger number of meals for a larva such that, when cated using four different source colonies. nutrition was sufficient, JH levels could rise, initiating major or gyne development. In this paper, we report the 2.3. Larval meal size effects of incubation temperature on larval development, larval meal size, worker brood tending and worker recruit- Larval appetites were quantified using video tech- ment to food. In addition, we report the effects of incu- nology (see Cassill and Tschinkel, 1995, 1999a). Food bation humidity on these same variables. for larval feeding consisted of a distilled water solution with 6% (w/v) casamino acids powder and 10% granu- 2. Materials and methods lated sugar (w/v). We tested temperatures within the range of viable brood production — 25°C, 30°C and 2.1. Source colonies 35°C. From one source colony, 8 g of workers were div- For temperature experiments, mature field colonies ided into three groups of 2 g each. Each group was were collected in Tallahassee during January of 1995 placed into an experimental nest and deprived of food D.L. Cassill, W.R. Tschinkel / Journal of Insect Physiology 46 (2000) 933–939 935 for 48 h. Larvae were collected using the same methods moist nest chambers (100% humidity). A colony was as reported above, then placed temporarily into a single starved for 48 h, then 2 g of workers (ෂ4,000) were ran- brood chamber and deprived of food for 12 h. Nests con- domly aspirated from the brood chamber and foraging taining workers were placed in the temperature-con- arena. Worker groups were halved and each half (1 g) trolled room to acclimate 12 h before the experiment was placed into either a dry brood chamber or a water- began.